YU4101A - Novi postupci za identifikovanje liganada i ciljnih biomolekula - Google Patents
Novi postupci za identifikovanje liganada i ciljnih biomolekulaInfo
- Publication number
- YU4101A YU4101A YU4101A YU4101A YU4101A YU 4101 A YU4101 A YU 4101A YU 4101 A YU4101 A YU 4101A YU 4101 A YU4101 A YU 4101A YU 4101 A YU4101 A YU 4101A
- Authority
- YU
- Yugoslavia
- Prior art keywords
- identification
- peptide
- methods
- rna
- identified
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 239000003446 ligand Substances 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 239000003596 drug target Substances 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 229940088598 enzyme Drugs 0.000 abstract 2
- 238000012216 screening Methods 0.000 abstract 2
- 229940122644 Chymotrypsin inhibitor Drugs 0.000 abstract 1
- 101710137926 Chymotrypsin inhibitor Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 240000005979 Hordeum vulgare Species 0.000 abstract 1
- 235000007340 Hordeum vulgare Nutrition 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 244000061456 Solanum tuberosum Species 0.000 abstract 1
- 235000002595 Solanum tuberosum Nutrition 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000003541 chymotrypsin inhibitor Substances 0.000 abstract 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 abstract 1
- 238000009509 drug development Methods 0.000 abstract 1
- 239000002532 enzyme inhibitor Substances 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 150000002611 lead compounds Chemical class 0.000 abstract 1
- 229940126601 medicinal product Drugs 0.000 abstract 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Pronalazak obezbedjuje postupke za identifikovanje bioloski aktivnih biomolekula. U jednom vidu pronalaska, jedan bioloski aktivan biomolekul kao sto je RNK ili neki peptid identifikovan je ugradnjom nasumice modifikovanih nukleotidnih sekvenci u jednu resetku koju sacinjava jedan modulator delovanja enzima, transformisanjem u sustini identicnih celija domacina napred dobijenim strukturama i pretrazivanjem tansformisanih celija da bi se identifikovale one kod kojih je promenjeno jedno unapred izabrano fenotipsko svojstvo. Potom se nasumice modifikovana DNK izdvaja iz fenotipski promenjenih celija i odredjuje se peptid i/ili RNK kodiran nasumice modifikovanom sekvencom. Sa svoje strane, partneri za medjusobno delovanje koji su moguci ciljni lekovi identifikovani su i izdvojeni koriscenjem peptida i/ili RNK kao dela afinitetnih reagensa. Jedna preporucljiva resetka je izvedena od krompirovog inhibitora I familije proteinskih inhibitora a kao primer prikazan je jecmeni inhibitor 2A (Cl-2A) himotripsina. Drugi vid pronalaska odnosi se na identifikaciju novih enzimskih inhibitora koristeci u sustini isti pristup, ali uz specificno pretrazivanje promena delovanja ciljnih enzima. Takodje su prikazani postupci izvodjenja relevantnih vektora transformacija i izrazavanja kao i postupci za identifikovanje vodecih jedinjenja i ciljnih lekova za primenu u razvoju lekova. Konacno, u opseg pronalaska takodje spada i postupak za pripremanje jednog lekovitog proizvoda.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199800956 | 1998-07-20 | ||
| US9486898P | 1998-07-29 | 1998-07-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| YU4101A true YU4101A (sh) | 2005-06-10 |
Family
ID=27675497
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| YU4101A YU4101A (sh) | 1998-07-20 | 1999-07-16 | Novi postupci za identifikovanje liganada i ciljnih biomolekula |
Country Status (2)
| Country | Link |
|---|---|
| YU (1) | YU4101A (sh) |
| ZA (1) | ZA200100195B (sh) |
-
1999
- 1999-07-16 YU YU4101A patent/YU4101A/sh unknown
-
2001
- 2001-01-08 ZA ZA200100195A patent/ZA200100195B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ZA200100195B (en) | 2002-01-08 |
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