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WO2021114086A1 - Dispositif de comptage de cellules - Google Patents

Dispositif de comptage de cellules Download PDF

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Publication number
WO2021114086A1
WO2021114086A1 PCT/CN2019/124300 CN2019124300W WO2021114086A1 WO 2021114086 A1 WO2021114086 A1 WO 2021114086A1 CN 2019124300 W CN2019124300 W CN 2019124300W WO 2021114086 A1 WO2021114086 A1 WO 2021114086A1
Authority
WO
WIPO (PCT)
Prior art keywords
cell
observation
tube
counting device
pool
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2019/124300
Other languages
English (en)
Chinese (zh)
Inventor
张豪
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Suzhou Sunscell Biotech Co Ltd
Original Assignee
Suzhou Sunscell Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Sunscell Biotech Co Ltd filed Critical Suzhou Sunscell Biotech Co Ltd
Priority to PCT/CN2019/124300 priority Critical patent/WO2021114086A1/fr
Publication of WO2021114086A1 publication Critical patent/WO2021114086A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle

Definitions

  • This application relates to the field of cell culture technology, and in particular to a cell counting device.
  • the existing cell counter When the existing cell counter performs the cell counting operation, firstly, the cell liquid is manually taken and the cell liquid is dropped on the counting plate, and then the counting plate is inserted into the counter (counter) to read the result information of the cell number.
  • Using this open method of manual liquid extraction requires many requirements for the cleanliness of the operating environment and operating steps, which increases the complex steps of sampling, and the existing counting method exposes all cultured cells to the air The cells are easily contaminated; the number of cells in the process of manual liquid extraction will also cause more losses and cause cell waste.
  • the purpose of this application is to overcome the problems that the existing cell counter requires manual sampling, which is easy to cause overall cell culture pollution and complicated operations, and to provide a real-time online cell counting device, including a cell circulation tube, a peristaltic pump, a restrictor valve, an observation block, Counter and display;
  • the cell circulation tube is provided with at least two openings for communicating with the cell culture bag;
  • the observation block is transparent, and an observation pool connected with the cell circulation tube is arranged inside the observation block; the observation pool includes an inlet end and an outlet end;
  • the peristaltic pump is connected to the cell circulation tube;
  • the flow limiting valve is connected to the cell circulation tube and is arranged at the inlet end of the observation block for limiting the thickness of the cell liquid surface entering the observation pool;
  • the counter includes an illuminating device and a microscope arranged at intervals in a longitudinal direction, and the counter is electrically connected with a display; the observation block is located between the illuminating device and the microscope.
  • the cell circulation tube includes two symmetrically arranged tube bodies, one end of the tube body communicates with the inlet end of the observation cell, and one end of the other tube body communicates with the outlet end of the observation cell.
  • the pipe body includes a plurality of straight pipes, elbows and connectors; each straight pipe and/or elbow is connected by the connector.
  • the tube body includes an inner tube and an outer tube; the inner tube is in communication with the observation tank.
  • the thickness of the cell liquid surface in the observation pool is 0-0.5 mm.
  • the thickness of the cell liquid surface in the observation pool is 0.1 mm.
  • observation pool is a U-shaped groove.
  • the beneficial effects of the present application are: through the cooperation of the sealed cell circulation tube and the observation block, the automatic closed circulation counting of the cell fluid is realized, and the cells do not need to be manually sampled and exposed to contamination in the operating environment, and at the same time, they are no longer manual counting. simple.
  • Figure 1 is a schematic diagram of the structure of the cell counting device of the present application.
  • Figure 2 is a schematic diagram of the structure of Figure 1 during use.
  • Fig. 3 is a schematic diagram of the structure of Fig. 1 with the counter and display removed.
  • Fig. 4 is a schematic diagram of the structure of the observation pool in Fig. 3.
  • Fig. 5 is a schematic diagram of the structure in Fig. 3 with the observation block removed.
  • the cell counting device shown in Figures 1 to 5 includes a cell circulation tube 1, a peristaltic pump 2, a flow limiting valve 3, an observation block 4, a counter 5 and a display 6;
  • the cell circulation tube 1 is provided with at least two openings for communicating with the cell culture bag 8;
  • the observation block 4 is transparent, preferably a non-toxic transparent plastic material, and is provided with an observation pool 7 communicating with the cell circulation tube 1; the observation pool 7 includes an inlet end and an outlet end; the observation pool 7 can be a U-shaped groove, or Can be other shapes.
  • the peristaltic pump 2 is connected to the cell circulation tube 1; the restrictor valve 3 is connected to the cell circulation tube 1 and is arranged at the inlet end of the observation block 4 to limit the thickness of the cell liquid surface entering the observation pool 7; the cell liquid in the observation pool 7
  • the thickness of the surface is 0-0.5mm, preferably 0.1mm, which can ensure that the cells in the cell liquid entering the observation pool 7 are in a single layer without superimposition, which is more conducive to counting.
  • the peristaltic pump 2 and the restrictor valve 3 can be directly selected from the relevant models purchased on the market and used in the cell culture system.
  • the counter 5 includes an illuminating device and a microscope arranged at intervals in the longitudinal direction, and the counter 5 is electrically connected to the display 6; the observation block 4 is located between the illuminating device and the microscope. Through the cooperation of the illumination device and the microscope (electron microscope), the cells in the observation cell 7 can be automatically counted and displayed on the display 6.
  • the counter 5 can also be other existing technologies, such as a method of taking cell images through a camera for counting.
  • the counter 5 of this embodiment is actually an existing product, so the specific implementation or structure will not be described again.
  • the cell circulation tube 1 can be a U-shaped tube.
  • the two ends of the tube are the openings respectively inserted into the cell culture bag 8.
  • a part of the tube is a transparent section and is set between the restrictor valve 3 and the peristaltic pump 2. It is necessary to use the counter 5 to photograph and count the transparent section.
  • the cell circulation tube 1 of this embodiment adopts another form, that is, the cell circulation tube 1 includes two symmetrically arranged tube bodies, as shown in Figures 3 and 5, one end of which is connected to the inlet end of the observation pool 7 , One end of the other tube is connected to the outlet end of the observation tank 7, and the inlet and outlet ends of the observation tank 7 depend on the direction of the peristaltic pump 2.
  • the pipe body may include a plurality of straight pipes 1.1, elbows 1.2, and connectors 1.3; each straight pipe 1.1 and/or elbow 1.2 is connected by the connector 1.3.
  • the length and arrangement of the tube body can be adjusted by the number and matching mode of the straight pipe 1.1 and the elbow 1.2; it can be applied to cell culture bags 8 of different sizes.
  • the tube body in order to reduce cell loss, includes an inner tube and an outer tube; the inner tube is in communication with the observation pool 7.
  • the outer tube protects the inner tube.
  • the straight pipe 1.1 is provided with multiple through holes.
  • the arrangement of the through holes can ensure that cells at different positions in the cell culture bag 8 can enter the observation pool 7 to ensure the accuracy of counting.
  • the cell counting device of this embodiment may further include a sealing plug matched with the cell culture bag 8 to ensure the tightness of the connection between the tube body and the cell culture bag 8.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Dispositif de comptage de cellules, comprenant un tube de circulation de cellules, une pompe péristaltique, une vanne de limitation d'écoulement, un bloc d'observation, un compteur et un dispositif d'affichage ; le tube de circulation cellulaire étant pourvu d'au moins deux ouvertures en communication avec un sac de culture cellulaire ; le bloc d'observation étant transparent, et un intérieur de celui-ci étant pourvu d'une piscine d'observation en communication avec le tube de circulation cellulaire ; la pompe péristaltique et la soupape de limitation d'écoulement étant reliées au tube de circulation de cellule ; le compteur comprenant un dispositif d'éclairage et un microscope, disposés verticalement à un intervalle l'un de l'autre, le compteur étant électriquement connecté à l'affichage ; le bloc d'observation étant situé entre le dispositif d'éclairage et le microscope. Au moyen de la combinaison du tube de circulation cellulaire et du bloc d'observation, un liquide cellulaire est mis en circulation et compté de manière automatique et étanche, les cellules ne sont pas contaminées ou perdues, et ne sont pas comptées manuellement, et le fonctionnement est simple.
PCT/CN2019/124300 2019-12-10 2019-12-10 Dispositif de comptage de cellules Ceased WO2021114086A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/124300 WO2021114086A1 (fr) 2019-12-10 2019-12-10 Dispositif de comptage de cellules

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/124300 WO2021114086A1 (fr) 2019-12-10 2019-12-10 Dispositif de comptage de cellules

Publications (1)

Publication Number Publication Date
WO2021114086A1 true WO2021114086A1 (fr) 2021-06-17

Family

ID=76328792

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2019/124300 Ceased WO2021114086A1 (fr) 2019-12-10 2019-12-10 Dispositif de comptage de cellules

Country Status (1)

Country Link
WO (1) WO2021114086A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012064878A2 (fr) * 2010-11-09 2012-05-18 The General Hospital Corporation Comptage de particules au moyen d'un compteur différentiel électrique
US20140011270A1 (en) * 2011-03-18 2014-01-09 Ge Healthcare Bio-Sciences Ab Flexible bag for cultivation of cells
CN204058472U (zh) * 2014-03-21 2014-12-31 姬广聚 一种全自动细胞计数器
CN107287119A (zh) * 2017-08-23 2017-10-24 湖南开启时代生物科技有限责任公司 一种细胞培养计数装置
FR3053051A1 (fr) * 2016-06-24 2017-12-29 Cellprothera Cassette et automate de culture cellulaire
WO2018137428A1 (fr) * 2017-06-06 2018-08-02 北京呈诺医学科技有限公司 Système de culture à haut rendement pour la production et la culture de cellules à grande échelle, et procédé d'application

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012064878A2 (fr) * 2010-11-09 2012-05-18 The General Hospital Corporation Comptage de particules au moyen d'un compteur différentiel électrique
US20140011270A1 (en) * 2011-03-18 2014-01-09 Ge Healthcare Bio-Sciences Ab Flexible bag for cultivation of cells
CN204058472U (zh) * 2014-03-21 2014-12-31 姬广聚 一种全自动细胞计数器
FR3053051A1 (fr) * 2016-06-24 2017-12-29 Cellprothera Cassette et automate de culture cellulaire
WO2018137428A1 (fr) * 2017-06-06 2018-08-02 北京呈诺医学科技有限公司 Système de culture à haut rendement pour la production et la culture de cellules à grande échelle, et procédé d'application
CN107287119A (zh) * 2017-08-23 2017-10-24 湖南开启时代生物科技有限责任公司 一种细胞培养计数装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WU, SIFANG ET AL.: "Introduction to Bioengineering Factory Design", 31 July 2007, CHINA LIGHT INDUSTRY PRESS, CN, ISBN: 978-7-5019-5920-4, article WU, SIFANG ET AL.: "Tubes and Valves", pages: 174 - 187, XP009528681 *

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