[go: up one dir, main page]

WO2019143055A1 - Composition comprenant un extrait de haricots fermentés obtenu à partir d'une fermentation par une souche d'aspergillus fumigatus, pour l'amélioration de soins cutanés - Google Patents

Composition comprenant un extrait de haricots fermentés obtenu à partir d'une fermentation par une souche d'aspergillus fumigatus, pour l'amélioration de soins cutanés Download PDF

Info

Publication number
WO2019143055A1
WO2019143055A1 PCT/KR2019/000187 KR2019000187W WO2019143055A1 WO 2019143055 A1 WO2019143055 A1 WO 2019143055A1 KR 2019000187 W KR2019000187 W KR 2019000187W WO 2019143055 A1 WO2019143055 A1 WO 2019143055A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
skin
extract
ppm
gum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2019/000187
Other languages
English (en)
Korean (ko)
Inventor
김민지
유정진
장유진
노윤화
이경은
강승현
박명삼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cosmax Inc
Original Assignee
Cosmax Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cosmax Inc filed Critical Cosmax Inc
Priority to CN201980000353.2A priority Critical patent/CN110278706B/zh
Publication of WO2019143055A1 publication Critical patent/WO2019143055A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7004—Monosaccharides having only carbon, hydrogen and oxygen atoms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A61K8/602—Glycosides, e.g. rutin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • the epidermis which is the outermost layer of the skin, is an organ that is always in contact with the external environment and serves as a protective barrier.
  • the skin barrier which exists in the stratum corneum of the epidermis, suppresses the loss of water and electrolytes, protects the human body from external physical damage and chemicals, and prevents bacteria and viruses from penetrating into the skin.
  • the stratum corneum is composed of dead keratinocyte protein and lipid existing between keratinocyte. It is known that lipid bilayer between keratinocyte plays the most important role in maintaining skin barrier.
  • Aspergillus cristatus is a microorganism dominated by Fuzhuan brick tea, a post fermented tea in China, and it is called a golden flower fungus because the Chinese form spores like the gold flower. .
  • A. cristatus has been used for a long time in the production of brick tea as a safe strain because it does not produce carcinogenic mycotoxins.
  • Yongyi Ge et al. (2016) reported that the presence and expression of mycotoxin gene was not observed in A. cristatus isolated from the blastocyst .
  • Soybean is one of cosmetic raw materials, and it helps skin to be enriched and helps to improve blood circulation, and it gives the skin vitality. In one room, it has the effect of enhancing the immunity of the human body, and it is known that the blood pressure is lowered, blood is removed, blood is removed from the blood, and prevention and improvement of tumor are prevented. Therefore, beans have been used as raw materials for cosmetics.
  • the present invention provides a composition for improving skin beauty, comprising an extract of fermented soybean fermented with a cristatus strain, gum, and glyceryl glucoside as an active ingredient.
  • the beans are produced by Aspergillus
  • the present invention provides a method for improving skin conditions comprising administering to an individual an effective amount of a composition comprising an extract of fermented soybean fermented with a cristatus strain, gum, and glyceryl glucoside.
  • compositions comprising an extract of a fermented soybean, a gum, and a glyceryl glucoside, wherein the soybean is fermented with a strain of Aspergillus cristatus , for the production of a skin condition improving agent.
  • the present invention provides a composition for improving skin beauty, comprising an extract of fermented soybean fermented with a cristatus strain, gum, and glyceryl glucoside as an active ingredient.
  • the skin cosmetic improvement or skin condition improvement may be skin moisturizing, barrier strengthening, or skin regeneration.
  • a composition comprising fermented soybean extract, gum, and glyceryl glucoside, wherein the soybean has been fermented with the A. cristatus strain, has a skin moisturizing, barrier strengthening, or skin regenerating effect . Therefore, the composition containing the fermented soybean extract, gum, and glyceryl glucoside can be used as a composition for skin moisturizing, barrier strengthening, or skin regeneration.
  • skin moisturizing may mean any action that maintains skin moisture or prevents moisture loss.
  • skin barrier enhancement may refer to any action that is located at the outermost position of the skin, thereby enhancing the function of the skin barrier to prevent moisture and nutritional loss.
  • skin regeneration may mean any action that, when a portion of the skin is lost, replenishes that part or promotes the proliferation of skin cells.
  • improvement may mean any action that at least reduces the degree of symptomatology, such as a condition associated with relief or treatment of a condition.
  • Aspergillus & cristatus is a kind of yeast fungus that forms spores like the golden flower and is also called golden flower fungus.
  • the above A. cristatus can be separated from food, soil, And may be, for example, isolated from Fuzhuan brick tea, a post fermented tea of China, for example.
  • the strain may be an Aspergillus cristatus Cosmax-GF strain (accession number: KCCM11820P).
  • the Aspergillus cristatus Cosmax-GF strain is a novel strain isolated from a zygotic body manufactured in China, specifically, diluting a zygotic body with a NaCl solution; Culturing the diluted zygotes in potato agar medium supplemented with chloramphenicol; And a step of selecting strains which form gold colonies in the medium.
  • the NaCl solution may contain from 0.50% w / w to 0.99% w / w, from 0.60% w / w to 0.95% w / w, from 0.70% w / w to 0.90% w / ), 0.80% (w / w) to 0.90 (w / w), or 0.84% (w / w) to 0.86% (w / w), for example 0.85% (w / w) NaCl solution.
  • the culture may be performed at a temperature of 20 to 40 ⁇ , 25 to 40 ⁇ , 25 to 35 ⁇ , 27 to 35 ⁇ , or 28 to 32 ⁇ .
  • the culture may be performed for 10 to 100 hours, 20 to 90 hours, 30 to 80 hours, 40 to 70 hours, 48 to 65 hours, or 48 to 60 hours.
  • soybean is a plant of dicotyledonous roots and legumes, which is also called “soybeans ".
  • the soybean can be used without limitation, and for example, various varieties such as black bean, black bean, brown bean, stain bean, and black bean can be used, but the present invention is not limited thereto.
  • the soybeans may be those obtained by a conventionally available method, for example, those sold in the market.
  • the soybean may be sterilized soybean, and the sterilization method may be carried out by a conventional method in the art.
  • the fermentation may be a solid fermentation.
  • solid fermentation may mean fermenting microorganisms in a solid state raw material having low water content.
  • the solid fermentation method may be advantageous in that it can be easily recovered without contamination of germs compared with the conventional liquid fermentation method or semi-solid fermentation method.
  • the fermented soybeans are soybean Aspergillus way may be one which loose Christa tooth (A. cristatus), specifically Aspergillus Christa tooth (A. cristatus) inoculated with the spore suspension on the solid fermentation .
  • the culture substrate can be converted to a functional material by extracellular enzyme of A. cristatus through the fermentation.
  • extracellular enzymes include, but are not limited to, alkaline phosphatase, esterase (C4), esterase lipase (C8), acid phosphatase, Beta-glucosaminidase, alpha-galactosidase, beta-galactosidase, beta-glucosidase, N-acetyl-beta-glucosaminidase ), Alpha-mannosidase, and the like, but are not limited thereto.
  • the fermentation temperature, time, and humidity may be suitably selected .
  • the fermented soybeans may be fermented at 25 to 40 ⁇ , 25 to 35 ⁇ , 28 to 35 ⁇ , 30 to 35 ⁇ , 31 to 34 ⁇ , 32 to 34 ⁇ or 33 ⁇ .
  • the temperature is higher than 35 ° C, the time required for the strain to form spores is increased, so that the enzyme activity and expression amount can be lowered.
  • the temperature is lower than 30 ° C, the enzyme reaction rate is slowed, Can be lowered.
  • the fermented soybeans may be cultured for 10 to 100 hours, 20 to 100 hours, 20 to 90 hours, 30 to 90 hours, 30 to 80 hours, 40 to 80 hours, 40 to 70 hours, 48 to 65 hours, To 65 hours, or 60 to 65 hours.
  • the fermentation time can be appropriately selected in accordance with the fermentation temperature so as to terminate until spores are formed in consideration of the growth of hyphae.
  • the fermented soybeans have a moisture content of 10 to 99.9%, a moisture content of 20 to 99.9%, a moisture content of 30 to 99.9%, a moisture content of 40 to 99.9%, a moisture content of 50 to 99.9%, a moisture content of 50 to 90%, a moisture content of 60 to 90% Humidity, 75-85% humidity, or 80-85% humidity conditions.
  • the humidity is less than 75%, the time required for the strain to form into the spore is increased, and the activity and expression amount of the enzyme can be lowered.
  • the humidity is higher than 85%, the moisture content is high, The growth of aerobic strain A. cristatus may be slowed and the incidence of contamination by other microorganisms may be increased.
  • the fermented soybeans may be fermented at 30 to 35 DEG C for 48 to 65 hours at 75 to 85% humidity.
  • the fermented soybeans may be the entire fermented soybean, a part thereof, or a material derived therefrom.
  • the fermented soybeans used for the extraction may be the whole, a part thereof, or a material derived therefrom, which may be pulverized or cut or suitably dried.
  • the fermented soybeans may be dried to have a moisture content of less than 20%, or less than 15%, for example less than 12%.
  • the extract may be extracted with a hydrophilic solvent, for example, alcohol, water, or a combination thereof.
  • the alcohol may be a compound having at least one-OH group of C1 to C10.
  • the alcohol may be a C1 to C6 alcohol, or a C3 to C6 polyhydric alcohol.
  • the alcohol may be methanol, ethanol, n-propanol, isopropanol, n-butanol, sec-butanol, isobutanol, tert-butanol, n-pentanol, n-hexanol or mixtures thereof.
  • the solvent may be, for example, a mixture of water and an alcohol, that is, an aqueous solution of an alcohol.
  • the alcohol concentration of the alcohol aqueous solution may be 1 to 100% (w / w), such as 1 to 99.5% (w / w), 10 to 100% (w / w), 20 to 100% (w / (W / w), 40 to 100% (w / w), 50 to 100% (w / w), 60 to 100% (W / w), 60 to 90% (w / w), 60 to 80% (w / w), 65 to 75% have.
  • the aqueous alcohol solution may be methanol, ethanol, or an aqueous butanol solution.
  • the extract may be an ethanol extract.
  • the extract may be extracted by a conventional method in the art such as warm extraction, pressure extraction, ultrasonic extraction, hot water extraction, reflux cooling extraction, subcritical extraction, or supercritical extraction.
  • the extraction solvent may be selected from the group consisting of 5 to 15 (volume / weight) times, such as 5 to 13 (volume / weight), 5 to 11 (volume / , 8 to 13 (volume / weight) times, 8 to 11 (volume / weight) times, or 9 to 11 (volume / weight) times.
  • 5 to 15 (volume / weight) times such as 5 to 13 (volume / weight), 5 to 11 (volume / , 8 to 13 (volume / weight) times, 8 to 11 (volume / weight) times, or 9 to 11 (volume / weight) times.
  • 5 to 15 (volume / weight) times such as 5 to 13 (volume / weight), 5 to 11 (volume / , 8 to 13 (volume / weight) times, 8 to 11 (volume / weight) times, or 9 to 11 (volume / weight) times.
  • 0.5 to 1.5 L of the extraction solvent may be added to 100 g of the whole fermented soybean, a part thereof, or a material derived therefrom.
  • the extraction may be carried out at a temperature of 4 ° C to 70 ° C, for example 4 ° C to 50 ° C, 4 ° C to 40 ° C, 4 ° C to 30 ° C, 10 ° C to 70 ° C, 15 ° C to 70 ° C, 10 C to 50 C, 10 C to 50 C, 4 to 40 C, 4 to 30 C, 10 to 40 C, 10 to 35 C, or 10 to 30 C or room temperature .
  • the extraction time can be appropriately selected according to the selected temperature and extraction method.
  • the extraction time may be from 1 hour to 3 days, from 1 hour to 2 days, from 1 hour to 1 day, from 5 hours to 3 days, from 5 hours to 2 days, from 5 hours to 1 day, from 10 hours to 3 days, from 15 Hour to 2 days, 15 hours to 36 hours, 18 hours to 30 hours, 1 day to 3 days, 1 day to 2 days, or 24 hours.
  • the fermented soybean powder can be extracted by ultrasonic extraction by immersing the fermented soybean powder in the extraction solvent for 2 to 4 hours, or by immersing in a solvent for 24 to 72 hours at room temperature.
  • the extraction may be one or more times, for example, one to five times, one to four times, one to three times, two to five times, or two to four times, each extraction being performed in the same way, It can be performed in other ways.
  • the above extraction can be performed by separating the plant residue and the extract by a known method such as filtration.
  • the extraction can also include removing the solvent from the resulting extract by known methods such as concentration under reduced pressure.
  • the extraction may also comprise preparing the dried extract by drying, such as lyophilization, of the resulting extract.
  • the extract may contain 1 ppm to 10000 ppm, 1 ppm to 1000 ppm, 1 ppm to 500 ppm, 10 ppm to 10000 ppm, 10 ppm to 1000 ppm, 10 ppm to 500 ppm, 50 ppm to 10000 ppm, 50 ppm to 1000 ppm , 50 ppm to 500 ppm, or 50 ppm to 150 ppm.
  • the extract may contain from 0.001% w / v to 80% w / v, such as 0.001% w / v to 50% w / v, 0.001% w / w / v), 0.001% (w / v) to 5% (w / v), 0.001% (w / v) to 1% (w / v) v), 0.01% (w / v) to 60% (w / v), 0.01% (w / v) to 40% (w / v), 0.01% , 0.01% (w / v) to 20% (w / v), 0.01% (w / v) to 10% (w / v), 0.01% May be included at a concentration of 0.01% (w / v) to 1% (w / v).
  • composition according to one embodiment of the present invention may contain, as an active ingredient, a fraction of the fermented soybean extract instead of the fermented soybean extract.
  • the composition according to one embodiment may further comprise a fraction of the extract of the fermented soybean.
  • fraction refers to a substance in which the extract of the fermented soybean is divided into its components, that is, a fractionated substance.
  • the fraction may be obtained by solvent fractionation.
  • the solvent fractionation may be a step of mixing the fermented soybean extract with a solvent and separating the substance present in the solvent.
  • the fraction may be a methylene chloride fraction, an ethyl acetate fraction, a butanol fraction, or a water fraction obtained by suspending the fermented soybean extract in water and sequentially fractionating the fraction with methylene chloride, ethyl acetate, butanol, and water.
  • the methylene chloride fraction is prepared by mixing the fermented soybean extract with water, mixing the mixture with methylene chloride, allowing the mixture to stand for a predetermined time, separating the methylene chloride layer, separating the separated methylene chloride layer , ≪ / RTI > Separation of the fractions may include removing methylene chloride from the methylene chloride layer.
  • the ethyl acetate fraction was obtained by mixing the remaining water after the methylene chloride fraction was separated with ethyl acetate, leaving it for a predetermined time, separating the ethyl acetate layer, and separating the fraction from the separated ethyl acetate layer Lt; / RTI > Separation of the fractions can include removal of the ethyl acetate from the ethyl acetate layer.
  • the butanol fraction may be obtained by mixing water remaining after the ethyl acetate fraction is separated again with butanol, leaving the butanol layer separated for a predetermined time, and separating the fraction from the separated butanol layer.
  • Separation of the fractions may include removing butanol from the butanol layer.
  • Conditions for such fractionation such as temperature conditions, pressure conditions, time, amount or concentration of solvent used, stirring, etc., may be as described for the extracts used to prepare the above fermented soybean extract.
  • the fractionation may be repeated one or more times, for example, 1 to 5 times.
  • Separation of the fractions may be accomplished by known methods such as filtration.
  • the fractionation may also include removing the solvent from the fraction obtained by known methods such as concentration under reduced pressure.
  • the fractionation may also include concentration and / or drying of the obtained fractions.
  • the concentration may be reduced pressure concentrated.
  • the drying may include vacuum drying, boiling drying, spray drying, room temperature drying or freeze drying.
  • the gum may be a polymer polysaccharide having elasticity, and the kind thereof is not limited. In one embodiment, it may be at least one selected from the group consisting of black locust bean gum, gum arabic, ghatti gum, karaya gum, guar gum, tragacanth gum, xanthan gum, carrageenan, mannan and glucomannan. In another embodiment, the black locust bean gum may be used.
  • Glyceryl glucoside (2-GG) may be used interchangeably with “Gluco-glycerol” and may mean a substance in which glycerol and glucose are combined.
  • the glyceryl glucoside is present in an amount ranging from 0.1 ppm to 1000 ppm, 0.1 ppm to 500 ppm, 0.1 ppm to 100 ppm, 0.1 ppm to 50 ppm, 1 ppm to 1000 ppm, 1 ppm to 500 ppm, 1 ppm to 100 ppm, ppm to 50 ppm, 5 ppm to 1000 ppm, 5 ppm to 500 ppm, 5 ppm to 100 ppm, 5 ppm to 50 ppm, or 5 ppm to 15 ppm.
  • the glyceryl glucoside may be present in an amount of from 0.0001% (w / v) to 10% (w / v), such as 0.0001% (w / v) to 5% (w / v) (w / v), 0.005% (w / v) to 10% (w / v), 0.005% (w / v) to 5% w / v), 0.001% (w / v) to 10% (w / v), 0.001% (w / v) v), 0.001% (w / v) to 0.1% (w / v), or 0.001% (w / v) to 0.01% (w / v).
  • composition according to one embodiment may be one that increases the expression of HAS3, or AQP3, and more specifically, that increases the expression of HAS3, or AQP3, to exhibit skin moisturizing effects.
  • composition according to one embodiment may be one which improves the skin barrier and enhances the skin barrier.
  • composition according to one embodiment may be one which promotes proliferation of fibroblasts, specifically, one that exhibits a skin regeneration effect by promoting proliferation of fibroblasts.
  • composition according to one embodiment of the present invention may exhibit skin moisturizing effect by maintaining skin moisture content for a long period of time.
  • composition according to one embodiment may comprise the extract as an effective amount or as an active ingredient.
  • the effective amount may be appropriately selected depending on the individual.
  • the severity of the disease or condition, the age, weight, health, sex, sensitivity of the individual to the extract, time of administration, route and rate of excretion, duration of administration, factors including other compositions combined or co- May be determined according to factors well known in the art, physiology or medical field.
  • the composition for improving skin care may further comprise a cosmetically, pharmaceutically or pharmaceutically acceptable excipient or carrier.
  • the carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof.
  • the excipient may be microcrystalline cellulose, lactose, low substituted hydroxy cellulose, or a combination thereof.
  • the disintegrant may be sodium starch glycolate, calcium monohydrogen phosphate anhydrous, or a combination thereof.
  • the binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or combinations thereof.
  • the lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.
  • the composition may be formulated into a parenteral dosage form.
  • the parenteral dosage form may be an injection or an external preparation for skin.
  • the external skin preparation may be a cream, a gel, an ointment, a skin emulsifier, a skin suspension, a transdermal patch, a drug-containing bandage, a lotion, or a combination thereof.
  • the external preparation for skin is usually used as a component used in external skin preparations such as cosmetics or medicines such as an aqueous component, an oily component, a powder component, an alcohol, a moisturizer, a thickener, an ultraviolet absorber, a whitening agent, an antiseptic, , Coloring agents, various skin nutrients, and the like can be appropriately blended as needed.
  • cosmetics or medicines such as an aqueous component, an oily component, a powder component, an alcohol, a moisturizer, a thickener, an ultraviolet absorber, a whitening agent, an antiseptic, , Coloring agents, various skin nutrients, and the like can be appropriately blended as needed.
  • the external preparation for skin may be a metal blocker such as sodium edetate, sodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate or gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridine, Vitamin C, ascorbic acid magnesium phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, fructose, fructose and other herbal medicines, various herbal medicines, tocopherol acetate, glycyrrhizic acid, Sugars such as trehalose and the like can also be appropriately compounded.
  • a metal blocker such as sodium edetate, sodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate or gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridine, Vitamin C, ascorbic acid magnesium phosphate, ascorbic acid glucoside, arbutin, kojic acid
  • the composition according to one embodiment may be a cosmetic composition.
  • the extract can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizer cream, a hand cream, a foundation, , A soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a suspension, a gel, a powder, a paste, a mask pack or a sheet or an aerosol composition.
  • Compositions of such formulations may be prepared according to methods conventional in the art.
  • the cosmetic composition may further contain preservatives, stabilizers, surfactants, solubilizers, moisturizers, emollients, ultraviolet absorbers, preservatives, bactericides, antioxidants, pH regulators, organic and inorganic pigments, fragrances, have.
  • the amount of the additional component such as the above-mentioned moisturizing agent and the like can be easily selected by those skilled in the art within a range not to impair the purpose and effect of the present invention.
  • the amount of the additional component is 0.001 to 5% by weight, % ≪ / RTI >
  • the composition according to one embodiment may be a food composition.
  • it can be formulated into a conventional health functional food formulation known in the art.
  • the food composition may be prepared by conventional formulations such as powders, granules, tablets, pills, capsules, suspensions, emulsions, syrups, And may be manufactured in the form of any health food such as confectionery, pizza, ramen, other noodles, gums, jellies, dairy products including ice cream, various soups, drinks, tea, drinks, alcoholic beverages and vitamin complexes.
  • a pharmaceutically acceptable carrier or excipient may be used for the formulation of the health food, and any carrier or additive known to be usable in the art for the preparation of the formulation to be produced may be used.
  • additives there can be used various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, Carbonating agents, and the like.
  • it may contain natural fruit juice, fruit juice beverage and flesh for the production of vegetable beverages.
  • additive components may be used independently or in combination, and the proportion of the additive may be 0.001 to 5 wt%, specifically 0.01 to 3 wt%, based on the total weight of the composition.
  • the content of the extract in the food composition may be suitably determined according to the intended use (prevention or improvement). Generally, it may contain 0.01 to 15% by weight of the total food weight, and when it is prepared as a beverage, it may contain 0.02 to 10 g, specifically 0.3 to 1 g, based on 100 mL.
  • the beverage may further contain ingredients other than the above extract, and may further contain various flavors or natural carbohydrates commonly used in beverages.
  • the natural carbohydrate include conventional sugars such as monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrin and cyclodextrin; and sugars such as xylitol, sorbitol, erythritol, Of sugar alcohols may be contained.
  • a natural flavoring agent e.g., tau Martin, stevia extract, etc.
  • a synthetic flavoring agent e.g., saccharin, aspartame, etc.
  • the ratio of the natural carbohydrate may be generally about 1 to 20 g, specifically about 5 to 12 g per 100 mL of beverage.
  • the composition according to one embodiment may be a pharmaceutical composition.
  • the pharmaceutical composition may be a pharmaceutical composition for preventing or treating a skin disease.
  • the skin disease may be a disease caused by skin barrier function impairment, skin aging, skin wounds, skin scars, or skin inflammation.
  • prevention includes inhibiting the occurrence of a disease.
  • treatment includes inhibiting, alleviating, or eliminating the development of the disease.
  • Damage to the skin barrier function may mean all the changes that appear on the skin due to the degradation or damage of the skin barrier function. For example, it may include, but is not limited to, increased wrinkles, dryness, dermatitis, atopic dermatitis, allergic dermatitis, acne and the like.
  • the pharmaceutical composition according to one embodiment of the present invention can prevent or treat damage to the skin barrier function by improving the skin barrier function by improving the skin layer of the skin.
  • the aging of the skin can mean all types and intangible changes that appear on the skin as the age goes on. For example, it may include, but is not limited to, increased skin wrinkles, drying, decreased wound healing ability, decreased skin immune function, and the like.
  • the pharmaceutical composition according to one embodiment of the present invention can prevent or treat skin aging by the effects of strengthening skin barrier, maintaining skin moisture, preventing loss of skin moisture, and regenerating skin.
  • the skin wound or skin scar can mean any disease that can be improved or remedied by maintaining the continuity of skin cells regenerated, differentiated, proliferating, interfering with the synthesis of scarring collagen and promoting collagen degradation. But are not limited to, scars such as bruises, abrasions, lacerations, scars such as hypertrophic scars, keloid scars, and the like.
  • the pharmaceutical composition according to one embodiment can prevent or treat skin wounds or skin scars due to the effects of skin moisture retention, prevention of skin moisture loss, and skin regeneration.
  • the skin inflammatory disease may mean all diseases caused by immune stimulation. But are not limited to, for example, atopic dermatitis, eczema, seborrheic dermatitis, psoriasis, acne, and the like.
  • the pharmaceutical composition according to one embodiment of the present invention can prevent or treat skin inflammatory diseases by the effects of maintaining skin moisture, preventing loss of skin moisture, strengthening skin barrier, and regenerating skin.
  • compositions according to one embodiment may be prepared according to conventional methods in the cosmetics, food, or pharmaceutical arts.
  • Another aspect provides a method of improving skin beauty of an individual comprising administering the composition to a subject.
  • the composition is the same as described above.
  • the method may be a method of efficiently maintaining skin moisture or preventing moisture loss of an individual, a method of enhancing a skin barrier function of an individual, or a method of improving or promoting skin regeneration of an individual.
  • administering and “transplanting” are used interchangeably and refer to the administration of a composition according to one embodiment to a subject by at least partial localization of the composition to a desired site, May refer to the placement of the composition according to embodiments. May be administered by any suitable route of delivery of at least a portion of the extract or extract components of the composition according to one embodiment to the desired location in the living individual.
  • Administration can be by any method known in the art. Administration may be by any means directly administered to a subject, such as by intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration, . The administration can be systemically or locally administered.
  • the subject may be a mammal, such as a person, a cow, a horse, a pig, a dog, a sheep, a goat, or a cat.
  • the subject may be an individual in need of skin aesthetic improvement, for example skin moisturizing, barrier enhancement, or skin regeneration effect.
  • the administration may comprise a combination of fermented soybean extract, gum, and glyceryl glucoside in an amount of 0.1 mg to 1,000 mg, such as 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg To 25 mg, 5 mg to 500 mg, 5 mg to 100 mg, or 1 mg to 5 mg, or 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, , 5 mg to 50 mg, 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg.
  • the dose may be variously prescribed depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, route of administration, excretion rate and responsiveness of the patient, These factors can be taken into account to appropriately adjust the dosage.
  • the number of doses may be at least once per day or within the range of clinically acceptable side effects and may be administered at one or two or more doses at the site of administration and may be administered daily or every two to five days The number of days of administration can be administered from one day to 30 days at one time of treatment. If necessary, the same treatment may be repeated after the appropriate time.
  • the dosage may be the same as that of a human per kg, or the dosage may be converted into a dose in terms of a volume ratio (for example, an average value) One dose may be administered.
  • composition according to one aspect of the present invention includes extracts of fermented soybean fermented with A. cristatus strain, gum, and glyceryl glucoside to improve skin moisturizing, barrier strengthening, and skin regeneration. It exhibits excellent effects and can be safely used because it does not irritate the skin.
  • FIG. 1 is a graph showing mRNA relative levels of the moisturizing factors HAS3 and AQP3.
  • Example 3 a group of treatments comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • FIG. 2 shows the results of immunohistochemical staining of artificial skin using AQP3 antibody to confirm the effect of increasing AQP3 expression in artificial skin.
  • Example 3 A group of treatments comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • FIG. 3 shows H & E staining results for confirming skin barrier improvement effect on artificial skin.
  • Example 3 A group treated with a composition comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • FIG. 4 is a result of observing the degree of proliferation of cells after 24 hours after scraping the cells as a result of confirming the skin cell regeneration effect.
  • Example 3 A group treated with a composition comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • Example 5 is a graph showing the amount of cells per hour (hr) as a result of confirming the skin cell regeneration effect.
  • Example 3 A group treated with a composition comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • FIG. 6 is a graph comparing skin moisture percentage (%) with time after applying Formulation Example 1 and Comparative Formulation Example 1.
  • FIG. 6 is a graph comparing skin moisture percentage (%) with time after applying Formulation Example 1 and Comparative Formulation Example 1.
  • Soybean was fermented with A. cristatus isolated in Example 1 to prepare fermented soybeans, and fermented soybean extract was prepared.
  • the beans used for the solid fermentation were prepared by washing after purchase in the market.
  • the washed soybeans were sterilized at 121 DEG C for 30 minutes.
  • A. cristatus of Example 1 which had been grown on a potato agar medium, was prepared as a spore suspension, and the suspension was added to the sterilized soybeans and incubated at 33 ° C. for 60 to 65 hours at 75 to 85% Lt; / RTI >
  • the solid fermented soybean was dried and pulverized at 60 ° C until the moisture content was less than 12%.
  • Powdered fermented soybeans (100 g) were placed in an extraction vessel, and immersed in 1 L of a 70 wt% ethanol aqueous solution as an extraction solvent and left at room temperature for 24 hours to obtain an extract.
  • the supernatant which was centrifuged at 3000 rpm for 10 minutes, was filtered through a 0.45 ⁇ m membrane to prepare a filtrate.
  • the filtrate was concentrated under reduced pressure using a rotary evaporator to obtain 9 to 11 g of concentrated extract.
  • Example 3 Preparation of composition for improving skin care
  • Example 2 (CAS No. 9000-40-2, Sigma-Aldrich), and glyceryl glucoside (CAS No. 22160-26-5, Jin Chem Co., Ltd.) prepared in Example 2,
  • the extract of Example 2 was contained at a concentration of 100 ppm, i.e., 0.01% (w / v), and contained at a concentration of locust bean black 500 ppm, i.e., 0.05% (w / v)
  • Glucoside was included at a concentration of 10 ppm, i.e. 0.001% (w / v).
  • human keratinocytes were inoculated in a 6-well cell culture dish at a density of 5 ⁇ 10 5 cells and cultured in a 5% CO 2 incubator at 37 ° C. for 24 hours. After the addition of the composition of Example 3, the cells were further cultured for 24 hours. The cells were recovered and RNA was isolated by adding 1 ml of trizol (RNA iso, DAKARA, Japan). RNA was quantified using Nanodrop 2000 (Thermo, USA), and cDNA was synthesized by reacting at 42 ° C for 55 minutes and at 70 ° C for 15 minutes (Reverse Transcriptase Mix, ELPIS biotech, Korea).
  • the synthesized cDNA was synthesized using a template of hyaluronic acid synthase-3 (HAS3), aquaporin 3 (AQP3) and a cyanine dye (SYBR Green supermix, Applied Biosystems, USA)
  • HAS3 and AQP3 genes were finally assessed by real-time PCR using a real-time PCR machine (Step One Plus, Applied Biosystems, USA) using real-time polymerase chain reaction.
  • the primer sequences used in the real-time PCR were shown in Table 2 below.
  • the real-time PCR reaction was carried out at 94 ° C for 5 minutes, after activation of the polymerase, at 40 ° C for 30 seconds at 95 ° C, 1 minute at 54 ° C, Respectively.
  • the expression level of the gene was finally analyzed by correction for the ⁇ -actin gene.
  • purified water containing hyaluronic acid at a concentration of 100 ppm was used.
  • FIG. 1 is a graph showing mRNA relative levels of the moisturizing factors HAS3 and AQP3.
  • Example 3 a group of treatments comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • the expression of HAS3 and AQP3 was greatly increased in the composition-treated group of Example 3 as compared with the group treated with 100 ppm of hyaluronic acid. Therefore, it was found that the composition containing the fermented soybean, locust bean gum, and glyceryl glucoside exhibited excellent effect of increasing the moisturizing function.
  • Example 3 UVB was applied to artificial skin to reduce AQP3.
  • the composition of Example 3 and the comparative example purified water containing 100 ppm of hyaluronic acid, were each treated for 3 days with artificial skin. Artificial skin was cut and fixed to paraffin. Immunohistochemical staining of skin tissue was performed using AQP3 antibody.
  • Example 3 The composition of Example 3 and the comparative example, purified water containing 100 ppm of hyaluronic acid, were each treated for 3 days with artificial skin. Artificial skin was cut and fixed to paraffin. H & E staining was performed to confirm the change of skin layer structure of artificial skin.
  • FIG. 2 shows the results of immunohistochemical staining of artificial skin using AQP3 antibody to confirm the effect of increasing AQP3 expression in artificial skin.
  • Example 3 A group of treatments comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • treatment of the composition of Example 3 showed an increase in the expression of AQP3 reduced by UVB, and the effect was found to be superior to that of 100 ppm of hyaluronic acid.
  • FIG. 3 shows H & E staining results for confirming skin barrier improvement effect on artificial skin.
  • Example 3 A group treated with a composition comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • the surface layer of the skin was significantly improved in the composition-treated group of Example 3 as compared with the non-treated group and the hyaluronic acid-treated group.
  • human fibroblasts were inoculated in a 96-well cell culture dish at a density of 2 ⁇ 10 4 cells and cultured in a 37, 5% CO 2 incubator for 24 hours. After the center of the cultured cells was scraped off using a cell scraper, the composition of Example 3 was added and cultured for 24 hours. The extent of cell proliferation was measured using an IncuCyte ZOOM machine and the amount of cells per time was quantitated.
  • FIG. 4 is a result of observing the degree of proliferation of cells after 24 hours after scraping the cells as a result of confirming the skin cell regeneration effect.
  • Example 3 A group treated with a composition comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • Example 5 is a graph showing the amount of cells per hour (hr) as a result of confirming the skin cell regeneration effect.
  • Example 3 A group treated with a composition comprising fermented soybeans, locust bean gum and glyceryl glucoside.
  • Example 3 As shown in Figs. 4 and 5, it was found that the composition of Example 3 was excellent in skin cell proliferation effect.
  • the cosmetic composition of Formulation Example 1 containing the skin-beauty-improving composition of Example 3 was prepared.
  • the cosmetic composition of Comparative Formulation Example 1 which did not contain the composition for skin-care improvement of Example 3 was prepared.
  • Table 3 below shows the specific components and contents of the cosmetic composition of Formulation Example 1 and Comparative Formulation Example 1.
  • FIG. 6 is a graph comparing skin moisture percentage (%) with time after applying Formulation Example 1 and Comparative Formulation Example 1.
  • FIG. 6 is a graph comparing skin moisture percentage (%) with time after applying Formulation Example 1 and Comparative Formulation Example 1.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Birds (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Molecular Biology (AREA)
  • Dermatology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention concerne une composition pour l'amélioration de soins cutanés. Une composition pour l'amélioration de soins cutanés selon un mode de réalisation présente d'excellents effets dans l'hydratation de la peau, l'amélioration de la barrière cutanée et la régénération de la peau, et ne provoque aucune irritation cutanée, grâce au fait qu'elle comprend : un extrait de haricots fermentés obtenu à partir de la fermentation par une souche d'Aspergillus fumigatus ; une gomme ; et du glucoside de glycéryle, et ainsi la composition peut être utilisée en toute sécurité.
PCT/KR2019/000187 2018-01-18 2019-01-07 Composition comprenant un extrait de haricots fermentés obtenu à partir d'une fermentation par une souche d'aspergillus fumigatus, pour l'amélioration de soins cutanés Ceased WO2019143055A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201980000353.2A CN110278706B (zh) 2018-01-18 2019-01-07 包含使用冠突曲霉菌株发酵的发酵大豆的提取物的皮肤美容改善用组合物

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2018-0006644 2018-01-18
KR1020180006644A KR102117218B1 (ko) 2018-01-18 2018-01-18 아스페르길루스 크리스타투스 균주로 발효시킨 발효 콩의 추출물을 포함하는 피부미용 개선용 조성물

Publications (1)

Publication Number Publication Date
WO2019143055A1 true WO2019143055A1 (fr) 2019-07-25

Family

ID=67302383

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2019/000187 Ceased WO2019143055A1 (fr) 2018-01-18 2019-01-07 Composition comprenant un extrait de haricots fermentés obtenu à partir d'une fermentation par une souche d'aspergillus fumigatus, pour l'amélioration de soins cutanés

Country Status (3)

Country Link
KR (1) KR102117218B1 (fr)
CN (1) CN110278706B (fr)
WO (1) WO2019143055A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110755472A (zh) * 2019-09-24 2020-02-07 萧敬皇 用于皮肤伤口愈合之大豆酦酵组合物及其制备方法
CN114292827A (zh) * 2021-12-28 2022-04-08 华熙生物科技股份有限公司 一种含甘油葡萄糖苷和酵母发酵产物的组合物及其制备方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112753952A (zh) * 2021-01-25 2021-05-07 陕西师范大学 一种具有通便功效的冠突散囊菌发酵大豆粉及其制备工艺
CN113249233B (zh) * 2021-06-29 2021-11-09 北京百奥茵诺生物科技有限公司 冠突散囊菌和菌剂及它们的应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050080085A (ko) * 2005-07-11 2005-08-11 조정일 발효콩 추출물을 이용한 건강 증진 목욕제 및 피부미용제
KR20090034241A (ko) * 2007-10-02 2009-04-07 동국대학교 산학협력단 천연 식물소재 및 한약재의 발효방법, 상기 방법에 의해서제조된 발효물 및 이를 함유하는 약학 조성물, 화장품조성물 및 식품 조성물
KR20120027654A (ko) * 2010-09-13 2012-03-22 (주)아모레퍼시픽 발효콩 추출 분획물을 유효 성분으로 포함하는 화장료 조성물
JP2013056864A (ja) * 2011-09-09 2013-03-28 Toyo Seito Kk カチオン化グリセリルグルコシドの混合物及び皮膚外用剤
KR20140018666A (ko) * 2012-08-03 2014-02-13 (주)아모레퍼시픽 발효콩 추출물을 함유하는 피부 외용제 조성물
KR20140103590A (ko) * 2013-02-18 2014-08-27 재단법인 하동녹차연구소 중국 복전차로부터 분리된 금화균인 유로티움 크리스타텀 균주
KR101533067B1 (ko) * 2013-05-14 2015-07-01 가천대학교 산학협력단 복합효모 대두 발효물을 함유하는 피부 주름 개선 및 피부 보습용 식품 및 화장료 조성물

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2893252B1 (fr) * 2005-11-17 2008-02-15 Engelhard Lyon Sa Extraits vegetaux stimulant has2
KR20130085012A (ko) * 2012-01-18 2013-07-26 주식회사 엘지생활건강 발효물을 포함하는 화장료 조성물 및 이의 제조방법
CN104171001A (zh) * 2014-07-29 2014-12-03 哈尔滨伟平科技开发有限公司 大豆保健饮料的制作方法
JP6292072B2 (ja) * 2014-08-01 2018-03-14 二村　芳弘 ヒアルロン酸合成酵素誘導作用を呈する脂肪酸誘導体及びその製造方法
KR101774342B1 (ko) * 2014-11-10 2017-09-04 코스맥스아이큐어 주식회사 하이드로겔 마스크팩 조성물, 이를 통해 제조된 하이드로겔 마스크팩 및 이의 제조방법
EP3231437B1 (fr) * 2014-12-09 2020-02-05 Nihon Sizen Hakkoh Co., Ltd. Inhibiteur de vieillissement

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050080085A (ko) * 2005-07-11 2005-08-11 조정일 발효콩 추출물을 이용한 건강 증진 목욕제 및 피부미용제
KR20090034241A (ko) * 2007-10-02 2009-04-07 동국대학교 산학협력단 천연 식물소재 및 한약재의 발효방법, 상기 방법에 의해서제조된 발효물 및 이를 함유하는 약학 조성물, 화장품조성물 및 식품 조성물
KR20120027654A (ko) * 2010-09-13 2012-03-22 (주)아모레퍼시픽 발효콩 추출 분획물을 유효 성분으로 포함하는 화장료 조성물
JP2013056864A (ja) * 2011-09-09 2013-03-28 Toyo Seito Kk カチオン化グリセリルグルコシドの混合物及び皮膚外用剤
KR20140018666A (ko) * 2012-08-03 2014-02-13 (주)아모레퍼시픽 발효콩 추출물을 함유하는 피부 외용제 조성물
KR20140103590A (ko) * 2013-02-18 2014-08-27 재단법인 하동녹차연구소 중국 복전차로부터 분리된 금화균인 유로티움 크리스타텀 균주
KR101533067B1 (ko) * 2013-05-14 2015-07-01 가천대학교 산학협력단 복합효모 대두 발효물을 함유하는 피부 주름 개선 및 피부 보습용 식품 및 화장료 조성물

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110755472A (zh) * 2019-09-24 2020-02-07 萧敬皇 用于皮肤伤口愈合之大豆酦酵组合物及其制备方法
CN114292827A (zh) * 2021-12-28 2022-04-08 华熙生物科技股份有限公司 一种含甘油葡萄糖苷和酵母发酵产物的组合物及其制备方法

Also Published As

Publication number Publication date
CN110278706A (zh) 2019-09-24
CN110278706B (zh) 2022-04-26
KR102117218B1 (ko) 2020-06-01
KR20190088286A (ko) 2019-07-26

Similar Documents

Publication Publication Date Title
KR102009633B1 (ko) 아스페르길루스 크리스타투스 균주로 고체발효시킨 발효 인삼의 추출물을 포함하는 피부미용 개선용 조성물
KR102316637B1 (ko) 병풀 유래 바실러스 서브틸리스 균주 및 그의 피부 상태 개선 용도
TWI842139B (zh) 用於改善皮膚狀態的組合物以及新型油鞘氨醇單胞菌菌株及其溶解產物、培養液、提取物
WO2019143055A1 (fr) Composition comprenant un extrait de haricots fermentés obtenu à partir d'une fermentation par une souche d'aspergillus fumigatus, pour l'amélioration de soins cutanés
KR101963572B1 (ko) 아스페르길루스 크리스타투스 균주로 발효시킨 발효 제비집의 추출물을 포함하는 피부미용 개선용 조성물
KR102046298B1 (ko) 아스페르길루스 크리스타투스 균주로 발효시킨 발효 표고버섯의 추출물을 포함하는 피부미용 개선용 조성물
KR102484422B1 (ko) 사막 선인장 추출물 및 락토바실러스 배양액을 포함하는 화장료 조성물
KR102019642B1 (ko) 아스페르길루스 크리스타투스 균주로 발효시킨 발효 콩의 추출물을 포함하는 피부미용 개선용 조성물
KR102495405B1 (ko) 마이크로코커스 루테우스 균주 및 그의 모발 또는 두피 상태 개선 용도
KR102176883B1 (ko) 복사나무 수지를 함유하는 피부 재생, 주름 개선, 또는 상처 치유용 조성물
WO2016056781A1 (fr) Composition pour prévenir la chute des cheveux ou stimuler la pousse des cheveux comprenant un extrait d'artemisia umbelliformis
KR102239415B1 (ko) 발효 삼칠근의 추출물을 포함하는 피부미용 개선용 조성물
KR102004150B1 (ko) 아스페르길루스 크리스타투스 균주로 발효시킨 발효 인삼의 추출물을 포함하는 피부미용 개선용 조성물
KR102114133B1 (ko) 알피나 골무꽃 추출물을 포함하는 탈모 방지 또는 육모 촉진용 조성물
KR101994618B1 (ko) 발효 지실의 추출물을 포함하는 피부 진정용 조성물
KR20220096031A (ko) 브레비바실러스 보스테렌시스 균주 및 그의 모발 또는 두피 상태 개선 용도
WO2016003120A1 (fr) Composition de blanchiment comprenant un extrait de scutellaria alpina
KR102810446B1 (ko) 천연 공융 용매로 추출한 추출물의 복합 유산균 발효물을 포함하는 화장료 조성물
KR102004149B1 (ko) 아스페르길루스 크리스타투스 균주로 발효시킨 발효 쌀의 추출물을 포함하는 피부미용 개선용 조성물
KR102334457B1 (ko) 마이크로박테리움 올레이보란스 균주 및 그의 피부 상태 개선 용도
KR102334456B1 (ko) 판토에아 아나나티스 균주 및 그의 피부 상태 개선 용도
WO2019139307A1 (fr) Composition pour le soin de la peau comprenant de la floculine
EP3127548A1 (fr) Composition comprenant un extrait d'essence d'absinthe alpine
WO2025264020A1 (fr) Utilisation de micro-organisme de l'espèce sphingomonas pour favoriser la pousse des cheveux
KR20260006370A (ko) 유산균 발효물, 사과껍질 추출물, 감잎 추출물, 및 밤 껍질 추출물을 포함하는 화장료 조성물

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 19740839

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 19740839

Country of ref document: EP

Kind code of ref document: A1