WO2019079912A1 - Method for controlling fungi and bacteria in bones and in the field of anatomical pathology; method for disinfecting and sanitising contaminated areas and/or surfaces; and uses - Google Patents
Method for controlling fungi and bacteria in bones and in the field of anatomical pathology; method for disinfecting and sanitising contaminated areas and/or surfaces; and usesInfo
- Publication number
- WO2019079912A1 WO2019079912A1 PCT/CL2018/050103 CL2018050103W WO2019079912A1 WO 2019079912 A1 WO2019079912 A1 WO 2019079912A1 CL 2018050103 W CL2018050103 W CL 2018050103W WO 2019079912 A1 WO2019079912 A1 WO 2019079912A1
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- Prior art keywords
- composition
- fungi
- bacteria
- copper
- bones
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/20—Copper
Definitions
- the present invention refers to compositions based on copper salts that have a fungicidal and bactericidal effect to be applied to surfaces and environments for disinfection and sanitization.
- pathological and forensic anatomy units since the risks associated with biological contaminants are known, among which bacteria and fungi stand out. The latter are very frequent, including their discovery in bones, which constitutes a risk associated both with health professionals working there, as well as with the judicial and forensic field due to the deterioration of the samples that are going to be subjected to various analyzes.
- the present invention provides a solution capable of inhibiting the growth of fungi and bacteria in units of pathological and forensic anatomy, using doses low enough to have no adverse effects on the DNA of the samples to be studied.
- the invention also relates to the use of these fungicidal and bactericidal compositions containing copper to be applied on biological material or on surfaces and environments contaminated with pathogens.
- Figure 1 Diffractogram of a composition according to the present invention.
- Figure 2 Infrared spectrum of a composition according to the present invention.
- Figure 3 Infrared spectrum of a composition according to the present invention.
- Figure 4 Graph of% growth inhibition of Penicillium sp.
- copper salt in some of the compositions of the invention may include one or more of the following monovalent or, preferably, divalent copper salts, including the salts of copper sulfate, copper sulfate monohydrate, sulfate copper dihydrate, copper sulfate trihydrate, copper sulphate pentahydrate, (Cu) i 5 (S04) 4 (OH) 22 6 (H 2 0) (rambsbeckite), copper sulfate tetrahydroxide ((Cu 2+ ) 3 S0 4 (OH) 4 ), double ammonium sulfate and copper hexahydrate ((NH 4 ) 2 (Cu (S0 4 ) x6H 2 0), copper chloride, copper nitrate, copper bicarbonate, copper oxychloride, copper borate, copper sulfamate, copper oxychloride sulfate, and other different types of copper sulfate hydroxides, such as (Cu) 4S04 (
- copper compounds which are suitable for the composition of the invention are copper hydroxide, cuprous oxide and cupric oxide
- double salt in some of the compositions of the invention includes the salts of Tutton which possess the general formula M 2 M (S0 4 ) 2 (H 2 0) 6 wherein the monovalent cation may include K, Rb, Cs, and especially ammonium (NH 4 + ).
- the divalent cation may include Mg, V, Cr, Mn, Fe, Co, Ni Zn, Cd and preferably Cu.
- Copper salts that are preferably used for solid formulations are copper salts that are largely insoluble in water, such as copper oxychloride or copper hydroxide.
- Copper salts that are preferably used for liquid or dispersed formulations are water soluble copper salts, such as copper sulfate pentahydrate.
- the present invention relates to a fungicidal and bactericidal composition
- a fungicidal and bactericidal composition comprising:
- the fungicidal and bactericidal composition further comprises copper sulfate trihydrate (CuS0 4 x3H 2 0).
- the fungicidal and bactericidal composition further comprises copper sulfate tetrahydroxide (Cu 3 S0 4 (OH) 4 ).
- the fungicidal and bactericidal composition further comprises amorphous material.
- the composition may also include lower amounts Ramsbeckite (Cu) i5 (S0 4 ) 4 (OH) 22 x6 (H 2 0).
- the double salt of ammonium sulfate and copper hexahydrate ((NH4) 2 Cu (S0 4 ) 2 6H 2 0) is in an amount between 20 and 40% by weight to the total composition.
- the copper sulfate pentahydrate salt (CuS0 4 x 5H 2 0) is present in an amount between 10 and 30% by weight with respect to the total composition.
- copper sulfate trihydrate (CuS0 4 x3H 2 0) is in an amount between 5 and 30% by weight with respect to the total composition.
- copper sulfate tetrahydroxide (Cu 3 S0 4 (OH) 4) is present in an amount between 2 and 15% by weight with respect to the total composition.
- the amorphous material in another preferred embodiment of the invention in the fungicidal and bactericidal composition is in an amount between 5 and 30% by weight with respect to the total composition.
- the fungicidal and bactericidal composition has a solubility in water, measured at 20 ° C, greater than 95% for a solution of 60 g of composition in 100 L of water.
- the fungicidal and bactericidal composition has a solubility in water, measured at 20 ° C, greater than 93% for a solution of 120 g of composition in 100 L of water.
- the present invention also relates to a method for the control of fungi and bacteria in bones and pathological anatomy, which comprises applying the composition described above, in all its modalities, on the surface to be analyzed.
- the composition in a preferred embodiment of the invention in the method for the control of fungi and bacteria in bones and pathological anatomy, is applied diluted in water. In a still more preferred embodiment of the invention in the method for the control of fungi and bacteria in bones and pathological anatomy, the diluted composition is applied by spraying on the surface to be disinfected.
- the present invention also relates to a method for disinfecting and sanitizing environments and / or surfaces contaminated with fungi and / or bacteria, which comprises applying the composition described above, in all its modalities, on the surface to be sanitized.
- the composition in the method for disinfecting and sanitizing environments and / or surfaces contaminated with fungi and / or bacteria, is applied diluted in water.
- the diluted composition is applied by spraying on the surface to be sanitized.
- the present invention is also directed to the use of the fungicidal and bactericidal composition of the composition described above, in all its modalities, to control fungi and bacteria in bones and pathological anatomy.
- the present invention is directed to the use of the fungicidal and bactericidal composition of the composition described above, in all its modalities, to disinfect and sanitize environments and / or surfaces contaminated with fungi and / or bacteria.
- X-ray diffraction is a technique in which a beam of X-rays, at a certain wavelength, is interacted with a crystalline substance. This methodology allows to identify the crystalline phase both in its qualitative and quantitative aspect, since every crystalline phase presents a fingerprint that is its diffraction diagram. Results obtained
- the samples of the composition have characteristic diffractograms of crystalline substances.
- Table 1 shows the identification of the crystalline phases found in the sample of a preferred composition according to the present invention.
- Figure 1 shows the diffractogram of a sample of the composition, in which each of the crystalline phases found in a composition according to the invention are identified.
- This method consists of crossing the sample at a certain wavelength with an infrared beam of light and determining the qualitative composition of a substance through absorbance.
- Each substance generates a unique absorbance curve for certain wavelengths.
- Figures 2 and 3 show the spectra obtained for a composition according to the invention compared with cupric sulfate and cupric sulfate pentahydrate, respectively.
- Table 2 determines the percentage of solubility of the composition according to the invention in water at different pH values.
- Example 1 Evaluation of the effectiveness of the composition according to the invention in its application for decontamination of bones in forensic units and pathological anatomy It is proposed to perform the efficacy tests in a composition of the invention based on copper salts in bones with high concentrations of fungal contamination.
- Mushroom sampling was carried out in a dependency of the Department of Legal Medicine of the Faculty of Medicine, University of Chile. Air samples of 50 liters were considered in 4 points of the room and a sample of 50 liters from the outside corridor. Samples were taken with an Air IDEAL® Biomerieux plate air impactor.
- the characteristic reference values for residential areas fluctuate between 10 to 490 CFU / m, depending on the season and conditions of the property. In open spaces the fungal load can vary between 150 and 250 CFU / m. So the area of bones is considered highly contaminated and high risk to health.
- Table 3 shows the results obtained, indicating the fungal load for each of the species identified in the sampling room and the corridor area. Due to the saturation of the instrument, these values are only referential and should not be considered as absolute values. The genus Penicillium predominated in all the samples.
- test conditions were carried out under controlled conditions that attempt to simulate the application of the compound in aerosol form on biological material contaminated with fungal agents. Test conditions:
- White biological material conidia of Penicillium sp. (Corresponds to the most represented fungal agent in the sample of the unit identified as "area of bone remains" of the Department of Legal Medicine of the Faculty of Medicine, University of Chile).
- Culture medium Sabouraud agar.
- White inoculum each plate of medium was inoculated with 195,000 conidia, in line with the high reported fungal load.
- each test was carried out in a closed chamber, with a volume of 24 liters, containing three plates of Sabouraud agar, previously inoculated with the conidia of
- Penicillium sp The tests consisted of the application in aerosol form of a certain volume of the composition according to the invention at different concentrations.
- Reading of results the effects of each treatment were evaluated after three days of incubation at room temperature. For each test, the growth area of the cultures was determined. The results are expressed as the percentage of inhibition of growth relative to the control without treating with the composition according to the invention.
- Table 4 specifies the details of the trial.
- the analyzed samples correspond to samples by contact with biological material or inert surfaces and only fungi were isolated, not showing bacterial development, at 96 hours of incubation and proceeds to isolate characteristic colonies of:
- Ciado sporium Ciado sporium.
- composition according to the invention were made at concentrations of 3.8% w / v, 9.6% w / v, 19.2% w / v and 38.4% w / v, and 2 trials were implemented:
- Mucor spp Table 7 Direct application composition according to the invention at a concentration of 38.4% w / v
- Table 8 Application to walls composition according to the invention at a concentration of 3.8% w / v
- composition according to the invention proves to be effective for the control of fungal growth.
- composition according to the invention in high concentration (15.2% w / v) is effective as a fungicide under the conditions tested.
- composition according to the invention For the concentrations of fungi studied, repeated doses of the composition according to the invention are required every 72 hours.
- Example 2 Determination of the effectiveness as a fungicide of the composition according to the present invention
- test conditions White biological material: conidia of Penicillium sp. (corresponding to the fungal agent most represented in the sample of the unit identified as "bone remains area" of the Department of Legal Medicine of the Faculty of Medicine, University of Chile). Culture medium: Sabouraud agar.
- each test was carried out in a closed chamber, with a volume of 24 liters, containing three plates of Sabouraud agar, previously inoculated with the conidia of Penicillium sp.
- the tests consisted of the application in aerosol form of a certain volume of a diluted solution of the composition at different concentrations. Table 10 details the details of each trial.
- Test 1 1.0 5.0
- composition according to the invention provides an effective solution for the control of fungi and bacteria in bones and pathological anatomy with doses high enough to effectively control fungi and bacteria, and at the same time low enough so as not to have effects on the DNA of the decontaminated material. , which is particularly due to the efficiency of the composition of the invention.
- composition developed allows its use in disinfection and industrial sanitization through fungicide and bactericidal effect in process rooms, fruit packing rooms, factories, warehouses, etc.
- composition of the present invention provides an effective solution in, for example, disinfection, decontamination and sanitization by bactericidal fungicidal effect in catastrophe management, hospitals, livestock industry, salmon industry, agricultural industry in general and for the food industry.
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Abstract
La presente invención se refiere a un método para controlar la presencia de hongos y bacterias en osamentas y anatomía patológica sin dañar las muestras para análisis de ADN, que comprende aplicar una solución de una composición que comprende sales de cobre, principalmente una sal doble de sulfato amonio y cobre hexahidratado ((NH4)2Cu(SO4)2X6H2O) y una sal de sulfato de cobre pentahidratado (CuSO4x5H2O). Asimismo, se divulga un método para la desinfección y sanitización de ambientes y/o superficies contaminados con hongos y/o bacterias por medio de la aplicación la mencionada composición.The present invention relates to a method for controlling the presence of fungi and bacteria in bones and pathological anatomy without damaging the samples for DNA analysis, which comprises applying a solution of a composition comprising copper salts, mainly a double salt of sulphate ammonium and copper hexahydrate ((NH4) 2Cu (SO4) 2X6H2O) and a copper sulfate salt pentahydrate (CuSO4x5H2O). Also, a method for the disinfection and sanitization of environments and / or surfaces contaminated with fungi and / or bacteria by means of the application of said composition is disclosed.
Description
MÉTODO PARA EL CONTROL DE HONGOS Y BACTERIAS EN OSAMENTAS Y ANATOMÍA PATOLÓGICA; MÉTODO PARA LA DESINFECCIÓN Y SANITIZACIÓN DE AMBIENTES Y/O SUPERFICIES CONTAMINADOS; Y USOS METHOD FOR THE CONTROL OF FUNGI AND BACTERIA IN OSAMENTS AND PATHOLOGICAL ANATOMY; METHOD FOR THE DISINFECTION AND SANITIZATION OF CONTAMINATED ENVIRONMENTS AND / OR SURFACES; AND USES
MEMORIA DESCRIPTIVA DESCRIPTIVE MEMORY
CAMPO DE APLICACIÓN SCOPE
La presente invención se refiere a composiciones en base a sales de cobre que poseen efecto fungicida y bactericida para ser aplicadas sobre superficies y ambientes para su desinfección y sanitización. The present invention refers to compositions based on copper salts that have a fungicidal and bactericidal effect to be applied to surfaces and environments for disinfection and sanitization.
ANTECEDENTES BACKGROUND
La eliminación de microorganismos causantes de enfermedades constituye un aspecto clave de higiene y seguridad asociado a distintos ambientes de las más distintas áreas del quehacer. The elimination of microorganisms that cause diseases is a key aspect of hygiene and safety associated with different environments of the most different areas of work.
Una de esas áreas que requiere un especial cuidado son las unidades de anatomía patológica y forense, puesto que son conocidos los riesgos asociados a contaminantes biológicos, dentro de los cuales destacan bacterias y hongos. Estos últimos son muy frecuentes, incluso su hallazgo en osamentas, lo cual constituye un riesgo asociado tanto a los profesionales del área de la salud que allí trabajan, como al campo judicial y forense debido al deterioro que sufren las muestras que se van a someter a diversos análisis. One of those areas that requires special care are the pathological and forensic anatomy units, since the risks associated with biological contaminants are known, among which bacteria and fungi stand out. The latter are very frequent, including their discovery in bones, which constitutes a risk associated both with health professionals working there, as well as with the judicial and forensic field due to the deterioration of the samples that are going to be subjected to various analyzes.
Poder contar con un producto formulado que permita controlar los riesgos asociados a la inocuidad del material en custodia es clave para las investigaciones sobre material biológico, especialmente a las asociadas con osamentas que presentan altas concentraciones de hongos y bacterias. En este contexto, la presente invención entrega una solución capaz de inhibir el crecimiento de hongos y bacterias en unidades de anatomía patológica y forense, empleando dosis lo suficientemente bajas como para no tener efectos adversos sobre el ADN de las muestras a estudiar. Being able to have a formulated product that allows controlling the risks associated with the safety of the material in custody is key to the research on biological material, especially those associated with bones that have high concentrations of fungi and bacteria. In this context, the present invention provides a solution capable of inhibiting the growth of fungi and bacteria in units of pathological and forensic anatomy, using doses low enough to have no adverse effects on the DNA of the samples to be studied.
RESUMEN DE LA INVENCIÓN SUMMARY OF THE INVENTION
La presente invención se refiere a composiciones que tienen efecto fungicida y bactericida que comprenden: The present invention relates to compositions having a fungicidal and bactericidal effect comprising:
una sal doble de sulfato de cobre y amonio; y a double salt of copper sulfate and ammonium; Y
una o más sales de cobre o compuestos de cobre. one or more copper salts or copper compounds.
La invención se refiere además al uso de estas composiciones fungicidas y bactericidas que contienen cobre para ser aplicadas sobre material biológico o sobre superficies y ambientes contaminados con agentes patógenos. The invention also relates to the use of these fungicidal and bactericidal compositions containing copper to be applied on biological material or on surfaces and environments contaminated with pathogens.
BREVE DESCRIPCIÓN DE LAS FIGURAS BRIEF DESCRIPTION OF THE FIGURES
Figura 1: Difractograma de una composición según la presente invención. Figure 1: Diffractogram of a composition according to the present invention.
Figura 2: Espectro infrarrojo de una composición según la presente invención. Figure 2: Infrared spectrum of a composition according to the present invention.
Figura 3: Espectro infrarrojo de una composición según la presente invención. Figure 3: Infrared spectrum of a composition according to the present invention.
Figura 4: Gráfico de % de inhibición del crecimiento de Penicillium sp. Figure 4: Graph of% growth inhibition of Penicillium sp.
DESCRIPCIÓN DETALLADA DE LA INVENCION DETAILED DESCRIPTION OF THE INVENTION
El término "sal de cobre" en algunas de las composiciones de la invención puede incluir uno o más de las siguientes sales de cobre monovalentes o, preferiblemente, divalentes entre las que se incluyen las sales sulfato de cobre, sulfato de cobre monohidratado, sulfato de cobre dihidratado, sulfato de cobre trihidratado, sulfato de cobre pentahidratado, (Cu)i5(S04)4(OH)22 6(H20) (rambsbeckita), tetrahidróxido de sulfato de cobre ((Cu2+)3S04(OH)4), sulfato doble de amonio y cobre hexahidratado ((NH4)2(Cu(S04)x6H20), cloruro de cobre, nitrato de cobre, bicarbonato de cobre, oxicloruro de cobre, borato de cobre, sulfamato de cobre, sulfato de oxicloruro de cobre, y otros diferentes tipos de hidróxidos de sulfates de cobre, tales como (Cu)4S04(OH)6 yThe term "copper salt" in some of the compositions of the invention may include one or more of the following monovalent or, preferably, divalent copper salts, including the salts of copper sulfate, copper sulfate monohydrate, sulfate copper dihydrate, copper sulfate trihydrate, copper sulphate pentahydrate, (Cu) i 5 (S04) 4 (OH) 22 6 (H 2 0) (rambsbeckite), copper sulfate tetrahydroxide ((Cu 2+ ) 3 S0 4 (OH) 4 ), double ammonium sulfate and copper hexahydrate ((NH 4 ) 2 (Cu (S0 4 ) x6H 2 0), copper chloride, copper nitrate, copper bicarbonate, copper oxychloride, copper borate, copper sulfamate, copper oxychloride sulfate, and other different types of copper sulfate hydroxides, such as (Cu) 4S04 (OH) 6 and
CuS04(OH)2. CuSO 4 (OH) 2 .
Otros compuestos de cobre que son adecuados para la composición de la invención son hidróxido de cobre, óxido cuproso y óxido cúprico Other copper compounds which are suitable for the composition of the invention are copper hydroxide, cuprous oxide and cupric oxide
El término sal doble en algunas de las composiciones de la invención incluye las sales de Tutton las cuales poseen la fórmula general M2M (S04)2(H20)6 en donde el catión monovalente pude incluir K, Rb, Cs, y especialmente amonio (NH4 +). El catión divalente puede incluir Mg, V, Cr, Mn, Fe, Co, Ni Zn, Cd y preferentemente Cu. The term "double salt" in some of the compositions of the invention includes the salts of Tutton which possess the general formula M 2 M (S0 4 ) 2 (H 2 0) 6 wherein the monovalent cation may include K, Rb, Cs, and especially ammonium (NH 4 + ). The divalent cation may include Mg, V, Cr, Mn, Fe, Co, Ni Zn, Cd and preferably Cu.
Las sales de cobre que se usan preferiblemente para formulaciones sólidas, es decir, formas pulverulentas o granuladas, son sales de cobre que son en gran medida insolubles en agua, tales como oxicloruro de cobre o hidróxido de cobre. Las sales de cobre que se usan preferiblemente para formulaciones líquidas o dispersadas son sales de cobre solubles en agua, tales como sulfato de cobre pentahidratado. Copper salts that are preferably used for solid formulations, i.e., powdery or granular forms, are copper salts that are largely insoluble in water, such as copper oxychloride or copper hydroxide. Copper salts that are preferably used for liquid or dispersed formulations are water soluble copper salts, such as copper sulfate pentahydrate.
DESCRIPCIÓN DE LAS MODALIDADES PREFERIDAS DESCRIPTION OF THE PREFERRED MODALITIES
La presente invención se refiere a una composición fungicida y bactericida que comprende: The present invention relates to a fungicidal and bactericidal composition comprising:
• una sal doble de sulfato amonio y cobre hexahidratado ((NH4)2Cu(S04)2x6H20); y • a double salt of ammonium sulfate and copper hexahydrate ((NH 4 ) 2 Cu (S0 4 ) 2 x6H 2 0); Y
• una sal de sulfato de cobre pentahidratado (CuS04x5H20). • a copper sulphate pentahydrate salt (CuS0 4 x5H 2 0).
En una realización preferida de la invención la composición fungicida y bactericida además comprende sulfato de cobre trihidratado (CuS04x3H20). In a preferred embodiment of the invention, the fungicidal and bactericidal composition further comprises copper sulfate trihydrate (CuS0 4 x3H 2 0).
En otra realización preferida de la invención la composición fungicida y bactericida además comprende tetrahidróxido de sulfato de cobre (Cu3S04(OH)4). In another preferred embodiment of the invention the fungicidal and bactericidal composition further comprises copper sulfate tetrahydroxide (Cu 3 S0 4 (OH) 4 ).
En otra realización preferida de acuerdo a la invención la composición fungicida y bactericida además comprende material amorfo. In another preferred embodiment according to the invention the fungicidal and bactericidal composition further comprises amorphous material.
En algunas realizaciones de la presente invención la composición puede además incluir menores cantidades Ramsbeckita (Cu)i5(S04)4(OH)22x6(H20). En una modalidad preferida de la invención en la composición fungicida y bactericida la sal doble de sulfato amonio y cobre hexahidratado ((NH4)2Cu(S04)2 6H20) se encuentra en una cantidad entre 20 y 40% en peso respecto a la composición total. In some embodiments of the present invention the composition may also include lower amounts Ramsbeckite (Cu) i5 (S0 4 ) 4 (OH) 22 x6 (H 2 0). In a preferred embodiment of the invention in the fungicidal and bactericidal composition the double salt of ammonium sulfate and copper hexahydrate ((NH4) 2 Cu (S0 4 ) 2 6H 2 0) is in an amount between 20 and 40% by weight to the total composition.
En otra modalidad preferida de la invención en la composición fungicida y bactericida la sal de sulfato de cobre pentahidratado (CuS04x5H20) se encuentra en una cantidad entre 10 y 30% en peso respecto a la composición total. In another preferred embodiment of the invention, in the fungicidal and bactericidal composition, the copper sulfate pentahydrate salt (CuS0 4 x 5H 2 0) is present in an amount between 10 and 30% by weight with respect to the total composition.
En otra modalidad preferida de la invención en la composición fungicida y bactericida el sulfato de cobre trihidratado (CuS04x3H20) se encuentra en una cantidad entre 5 y 30% en peso respecto a la composición total. In another preferred embodiment of the invention in the fungicidal and bactericidal composition copper sulfate trihydrate (CuS0 4 x3H 2 0) is in an amount between 5 and 30% by weight with respect to the total composition.
En otra modalidad preferida de la invención en la composición fungicida y bactericida el tetrahidróxido de sulfato de cobre (Cu3S04(OH)4) se encuentra en una cantidad entre 2 y 15% en peso respecto a la composición total. In another preferred embodiment of the invention, in the fungicidal and bactericidal composition, copper sulfate tetrahydroxide (Cu 3 S0 4 (OH) 4) is present in an amount between 2 and 15% by weight with respect to the total composition.
En otra modalidad preferida de la invención en la composición fungicida y bactericida el material amorfo se encuentra en una cantidad entre 5 y 30% en peso respecto a la composición total. In another preferred embodiment of the invention in the fungicidal and bactericidal composition the amorphous material is in an amount between 5 and 30% by weight with respect to the total composition.
En una modalidad preferida de la invención la composición fungicida y bactericida presenta una solubilidad en agua, medida a 20°C, mayor al 95% para una solución de 60 g de composición en 100 L de agua. In a preferred embodiment of the invention, the fungicidal and bactericidal composition has a solubility in water, measured at 20 ° C, greater than 95% for a solution of 60 g of composition in 100 L of water.
En una modalidad preferida de la invención la composición fungicida y bactericida presenta una solubilidad en agua, medida a 20°C, mayor al 93% para una solución de 120 g de composición en 100 L de agua. In a preferred embodiment of the invention, the fungicidal and bactericidal composition has a solubility in water, measured at 20 ° C, greater than 93% for a solution of 120 g of composition in 100 L of water.
La presente invención también se refiere a un método para el control de hongos y bacterias en osamentas y anatomía patológica, que comprende aplicar la composición antes descrita, en todas sus modalidades, sobre la superficie a analizar. The present invention also relates to a method for the control of fungi and bacteria in bones and pathological anatomy, which comprises applying the composition described above, in all its modalities, on the surface to be analyzed.
En una modalidad preferida de la invención en el método para el control de hongos y bacterias en osamentas y anatomía patológica, la composición se aplica diluida en agua. En una modalidad aún más preferida de la invención en el método para el control de hongos y bacterias en osamentas y anatomía patológica, la composición diluida se aplica por aspersión sobre la superficie a desinfectar. In a preferred embodiment of the invention in the method for the control of fungi and bacteria in bones and pathological anatomy, the composition is applied diluted in water. In a still more preferred embodiment of the invention in the method for the control of fungi and bacteria in bones and pathological anatomy, the diluted composition is applied by spraying on the surface to be disinfected.
La presente invención también se refiere a un método para la desinfección y sanitización de ambientes y/o superficies contaminadas con hongos y/o bacterias, que comprende aplicar la composición antes descrita, en todas sus modalidades, sobre la superficie a sanitizar. The present invention also relates to a method for disinfecting and sanitizing environments and / or surfaces contaminated with fungi and / or bacteria, which comprises applying the composition described above, in all its modalities, on the surface to be sanitized.
En una modalidad preferida de la invención en el método para la desinfección y sanitización de ambientes y/o superficies contaminadas con hongos y/o bacterias la composición se aplica diluida en agua. In a preferred embodiment of the invention, in the method for disinfecting and sanitizing environments and / or surfaces contaminated with fungi and / or bacteria, the composition is applied diluted in water.
En una modalidad aún más preferida de la invención en el método para la desinfección y sanitización de ambientes y/o superficies contaminadas con hongos y/o bacterias, la composición diluida se aplica por aspersión sobre la superficie a sanitizar. In an even more preferred embodiment of the invention in the method for disinfecting and sanitizing environments and / or surfaces contaminated with fungi and / or bacteria, the diluted composition is applied by spraying on the surface to be sanitized.
La presente invención también está dirigida al uso de la composición fungicida y bactericida la composición antes descrita, en todas sus modalidades, para controlar hongos y bacterias en osamentas y anatomía patológica. The present invention is also directed to the use of the fungicidal and bactericidal composition of the composition described above, in all its modalities, to control fungi and bacteria in bones and pathological anatomy.
Por último, la presente invención está dirigida al uso de la composición fungicida y bactericida la composición antes descrita, en todas sus modalidades, para desinfectar y sanitizar ambientes y/o superficies contaminados con hongos y/o bacterias. Finally, the present invention is directed to the use of the fungicidal and bactericidal composition of the composition described above, in all its modalities, to disinfect and sanitize environments and / or surfaces contaminated with fungi and / or bacteria.
ANÁLISIS DE LA COMPOSICIÓN DE LA INVENCIÓN ANALYSIS OF THE COMPOSITION OF THE INVENTION
Para determinar cada uno de los componentes que comprende la composición de la invención se realizaron estudios de Difracción de rayos X y Espectroscopia infrarroja. X-ray diffraction and infrared spectroscopy studies were carried out to determine each of the components comprising the composition of the invention.
DIFRACCIÓN DE RAYOS X X-RAY DIFFRACTION
La difracción de rayos X es una técnica en la que se hace interaccionar un haz de rayos X, a una determinada longitud de onda, con una sustancia cristalina. Esta metodología permite identificar la fase cristalina tanto en su aspecto cualitativo como cuantitativo, ya que toda fase cristalina presenta una huella dactilar que es su diagrama de difracción. Resultados obtenidos X-ray diffraction is a technique in which a beam of X-rays, at a certain wavelength, is interacted with a crystalline substance. This methodology allows to identify the crystalline phase both in its qualitative and quantitative aspect, since every crystalline phase presents a fingerprint that is its diffraction diagram. Results obtained
Las muestras de la composición presentan difractogramas característicos de sustancias cristalinas. The samples of the composition have characteristic diffractograms of crystalline substances.
La Tabla 1 muestra la identificación de las fases cristalinas encontradas en la muestra de una composición preferida según la presente invención. Table 1 shows the identification of the crystalline phases found in the sample of a preferred composition according to the present invention.
Tabla 1 Table 1
La Figura 1 muestra el difractograma de una muestra de la composición, en la cual se identifican cada una de las fases cristalinas encontradas en una composición según la invención. Figure 1 shows the diffractogram of a sample of the composition, in which each of the crystalline phases found in a composition according to the invention are identified.
ESPECTROSCOPIA INFRARROJA INFRARED SPECTROSCOPY
Este método consiste en atravesar con un haz de luz infrarroja la muestra a determinada longitud de onda y determinar a través de la absorbancia la composición cualitativa de una sustancia.This method consists of crossing the sample at a certain wavelength with an infrared beam of light and determining the qualitative composition of a substance through absorbance.
Cada sustancia genera una curva de absorbancia única para determinadas longitudes de onda.Each substance generates a unique absorbance curve for certain wavelengths.
Las Figuras 2 y 3 muestran los espectros obtenidos para una composición según la invención comparada con sulfato de cúprico y sulfato de cúprico pentahidratado, respectivamente. Figures 2 and 3 show the spectra obtained for a composition according to the invention compared with cupric sulfate and cupric sulfate pentahydrate, respectively.
Conclusiones de los análisis de difracción de rayos X y de espectro IR: Conclusions of X-ray diffraction and IR spectrum analysis:
• La muestra analizada está caracterizada por distintos compuestos de sulfato de cobre y material amorfo: • The sample analyzed is characterized by different copper sulphate compounds and amorphous material:
a. Ammonium Copper Sulfate Hydrate; to. Ammonium Copper Sulfate Hydrate;
b. Bonattite; b. Bonattite;
c. Chalcanthite, syn; c. Chalcanthite, syn;
d. Ramsbeckite; e. Antlerite; d. Ramsbeckite; and. Antlerite;
f. Copper Sulfate Hydrate; y F. Copper Sulfate Hydrate; Y
g. Material Amorfo. g. Amorphous material
• Los espectros infrarrojos de la composición de la invención comparados con el espectro infrarrojo del sulfato de cobre pentahidratado (material de partida) y de sulfato de cobre II, permiten establecer que son distintos, existiendo una señal característica de la composición según la invención a 1.400 cm"1, la cual corresponde a la señal del amonio.• The infrared spectra of the composition of the invention compared with the infrared spectrum of copper sulphate pentahydrate (starting material) and copper sulfate II, make it possible to establish that they are different, there being a characteristic signal of the composition according to the invention at 1400 cm "1 , which corresponds to the ammonium signal.
• Al secar la muestra, el grupo funcional característico de esta formulación, amonio, se mantiene estable como sulfato de cobre amonio hidratado. • When the sample is dried, the functional group characteristic of this formulation, ammonium, remains stable as hydrous ammonium copper sulfate.
• Por medio de los espectros IR, se puede establecer que la formación de una composición distinta obtenida a partir de sulfato de cobre pentahidratado. • Through the IR spectra, it can be established that the formation of a different composition obtained from copper sulphate pentahydrate.
DETERMINACIÓN DE LA SOLUBILIDAD DE LA COMPOSICIÓN SEGÚN LA INVENCIÓN DETERMINATION OF THE SOLUBILITY OF THE COMPOSITION ACCORDING TO THE INVENTION
El análisis de la Tabla 2 determina el porcentaje de solubilidad de la composición según la invención en agua a diferentes valores de pH. The analysis of Table 2 determines the percentage of solubility of the composition according to the invention in water at different pH values.
Tabla 2 Table 2
8 96,3 94,4 8 96.3 94.4
9 95,9 94,3 9 95.9 94.3
9 95,6 96,6 9 95.6 96.6
10 95,8 94,1 10 95.8 94.1
10 95,7 93,9 10 95.7 93.9
EJEMPLOS DE APLICACIÓN EXAMPLES OF APPLICATION
Ejemplo 1: Evaluación de la eficacia de la composición según la invención en su aplicación para descontaminación de osamentas en unidades forenses y anatomía patológica Se plantea efectuar las pruebas de eficacia en una composición de la invención en base a sales de cobre en osamentas con altas concentraciones de contaminación por hongos. Example 1: Evaluation of the effectiveness of the composition according to the invention in its application for decontamination of bones in forensic units and pathological anatomy It is proposed to perform the efficacy tests in a composition of the invention based on copper salts in bones with high concentrations of fungal contamination.
Para este estudio se utilizó la siguiente composición: For this study the following composition was used:
Metodología Methodology
Se realizó el muestreo de hongos en una dependencia del Departamento de Medicina Legal de la Facultad de Medicina, Universidad de Chile. Se consideraron muestras de aire de 50 litros en 4 puntos de la habitación y una muestra de 50 litros desde el pasillo exterior. Las muestras fueron tomadas con un impactador de aire en placa Air IDEAL® Biomerieux. Mushroom sampling was carried out in a dependency of the Department of Legal Medicine of the Faculty of Medicine, University of Chile. Air samples of 50 liters were considered in 4 points of the room and a sample of 50 liters from the outside corridor. Samples were taken with an Air IDEAL® Biomerieux plate air impactor.
A pesar del pequeño volumen de muestra por punto (50 litros), se superó la capacidad del equipo, saturando al impactador. Lo que permitió establecer que el área muestreada presenta una carga fúngica total > 32.642 UFC/m (unidades formadoras de colonias/metro cúbico) valor considerado de sobreexposición aguda. Despite the small sample volume per point (50 liters), the capacity of the equipment was exceeded, saturating the impactor. What allowed to establish that the sampled area presents a total fungal load> 32,642 CFU / m (colony forming units / cubic meter) considered a value of acute overexposure.
Los valores de referencia característicos para áreas residenciales fluctúan entre 10 a 490 UFC/m , dependiendo de la estación del año y condiciones del inmueble. En los espacios abiertos la carga fúngica puede variar entre 150 y 250 UFC/m . Por lo que la zona de osamentas se considera altamente contaminada y de alto riesgo para salud. The characteristic reference values for residential areas fluctuate between 10 to 490 CFU / m, depending on the season and conditions of the property. In open spaces the fungal load can vary between 150 and 250 CFU / m. So the area of bones is considered highly contaminated and high risk to health.
En la Tabla 3 se muestran los resultados obtenidos, se indica la carga fúngica para cada una de las especies identificadas en la habitación de muestreo y el área del pasillo. Debido a la saturación del instrumento, estos valores son sólo referenciales y no deben ser considerados como valores absolutos. El género Penicillium predominó en todas las muestras. Table 3 shows the results obtained, indicating the fungal load for each of the species identified in the sampling room and the corridor area. Due to the saturation of the instrument, these values are only referential and should not be considered as absolute values. The genus Penicillium predominated in all the samples.
Tabla 3: Carga fúngica por punto de muestreo y especie locaíizacíór» Especies fúngícas UFC/m Table 3: Fungal load per sampling point and species locaíizacíór »Fungic species CFU / m
Penicillium spp. 27.746 Penicillium spp. 27.746
Cladosporium spp. > 4.080 Cladosporium spp. > 4.080
Habitación A spergilius fiavus > 718 Room A spergilius fiavus> 718
AspergiH us niger > 65 AspergiH us niger> 65
Mucor s p. > 33 Mucor s p. > 33
Penicillium spp, 29,835 Penicillium spp, 29,835
Cladosporium spp, > 2,807 Cladosporium spp,> 2,807
Pasillo Hall
Aspergillus fiavus Aspergillus fiavus
(punto 5} (point 5}
Aspergiiius niger Aspergiiius niger
Mucor spp. Mucor spp.
Efectividad de la composición según la invención Effectiveness of the composition according to the invention
Los ensayos se realizaron bajo condiciones controladas que intentan simular la aplicación del compuesto en forma de aerosol sobre material biológico contaminado con agentes fúngicos. Condiciones de los ensayos: The tests were carried out under controlled conditions that attempt to simulate the application of the compound in aerosol form on biological material contaminated with fungal agents. Test conditions:
Material biológico blanco: conidios de Penicillium sp. (Corresponde al agente fúngico más representado en el muestreo de la dependencia identificada como "zona de restos óseos" del Departamento de Medicina Legal de la Facultad de Medicina, Universidad de Chile). White biological material: conidia of Penicillium sp. (Corresponds to the most represented fungal agent in the sample of the unit identified as "area of bone remains" of the Department of Legal Medicine of the Faculty of Medicine, University of Chile).
Medio de cultivo: agar Sabouraud. Inoculo blanco: cada placa de medio fue inoculado con 195.000 conidios, en consonancia con la alta carga fúngica reportada. Culture medium: Sabouraud agar. White inoculum: each plate of medium was inoculated with 195,000 conidia, in line with the high reported fungal load.
Tratamiento: cada ensayo se realizó en una cámara cerrada, con un volumen de 24 Litros, conteniendo tres placas de agar Sabouraud, previamente inoculadas con los conidios de Treatment: each test was carried out in a closed chamber, with a volume of 24 liters, containing three plates of Sabouraud agar, previously inoculated with the conidia of
Penicillium sp. Los ensayos consistieron en la aplicación en forma de aerosol, de un determinado volumen de la composición según la invención a diferentes concentraciones.Penicillium sp. The tests consisted of the application in aerosol form of a certain volume of the composition according to the invention at different concentrations.
Lectura de resultados: los efectos de cada tratamiento fueron evaluados después de tres días de incubación a temperatura ambiente. Para cada ensayo se determinó el área de crecimiento de los cultivos. Los resultados se expresan como el porcentaje de inhibición del crecimiento respecto al control sin tratar con la composición según la invención.Reading of results: the effects of each treatment were evaluated after three days of incubation at room temperature. For each test, the growth area of the cultures was determined. The results are expressed as the percentage of inhibition of growth relative to the control without treating with the composition according to the invention.
En la Tabla 4 se especifican los detalles del ensayo. Table 4 specifies the details of the trial.
Tabla 4: Condiciones de cada ensayo de inhibición del crecimiento con la composición de la invención Table 4: Conditions of each growth inhibition test with the composition of the invention
Los resultados mostraron que la aplicación de 5 mi (cada aplicación de 1 mi en forma de aerosol) de la composición según la invención de 15,2 % p/v redujo en un 98% el crecimiento de Penicillium sp. The results showed that the application of 5 ml (each application of 1 ml in the form of an aerosol) of the composition according to the invention of 15.2% w / v reduced the growth of Penicillium sp by 98%.
Aplicación de la composición según la invención Application of the composition according to the invention
Para evaluar las condiciones de uso y hacerlas comparables a las condiciones habituales de manejo de osamentas, se procedió a verificar mediante cultivo de láminas, Envirocheck, la presencia de distintos agentes biológicos y luego se aislaron en láminas separadas para ser expuestas a la composición según la invención a distintas concentraciones por asperjado directo y en las paredes del envase. In order to evaluate the conditions of use and make them comparable to the usual conditions of bone management, we proceeded to verify by means of leaf culture, Envirocheck, the presence of different biological agents and then isolated in separate sheets to be exposed to the composition according to the invention at different concentrations by direct spraying and on the walls of the container.
En el análisis de bacterias y hongos por Envirocheck de contacto, se estudiaron los siguientes agentes: In the analysis of bacteria and fungi by Envirocheck contact, the following agents were studied:
Tabla 5: Tipos de agentes patógenos estudiados Table 5: Types of pathogens studied
Las muestras analizadas corresponden a muéstreos por contacto con material biológico o superficies inertes y sólo se aislaron hongos, no presentando desarrollo bacteriano, a las 96 horas de incubación y se procede a aislar colonias características de: The analyzed samples correspond to samples by contact with biological material or inert surfaces and only fungi were isolated, not showing bacterial development, at 96 hours of incubation and proceeds to isolate characteristic colonies of:
- Aspergillus niger. - Aspergillus niger.
Aspergillus flavus. Aspergillus flavus.
Ciado sporium. Ciado sporium.
Mucor spp. Mucor spp.
Las aplicaciones directas de la composición según la invención se hicieron a concentraciones 3,8% p/v, 9,6% p/v, 19,2% p/v y 38,4% p/v, y se implementaron 2 ensayos: The direct applications of the composition according to the invention were made at concentrations of 3.8% w / v, 9.6% w / v, 19.2% w / v and 38.4% w / v, and 2 trials were implemented:
i. Control en que no se utiliza agente controlador ni directamente ni en las paredes al interior del envase y se procede a sembrar las 4 cepas en placas de agar rosa bengala cloranfenicol, y luego se observa su desarrollo a distintos tiempos. i. Control in which no controlling agent is used directly or on the walls inside the container and the 4 strains are planted in pink agar plates chloramphenicol bengal, and then its development is observed at different times.
ii. Ensayo en que se siembran estas 4 cepas en placas de agar rosa de bengala cloranfenicol, en condiciones ambientales estables, temperatura ambiente, y se observa a distintos tiempos a crecimiento de colonias. Cuando éstas se evidencian (96 horas) se procede a la aplicación de 2 mi de mezcla de la composición según la invención a las concentraciones antes mencionadas, en forma de aerosol, para nuevamente observar desarrollo de hongos y reaplicar en caso de crecimiento de hongos. ii. Test in which these 4 strains are planted in pink agar plates of chloramphenicol, under stable environmental conditions, room temperature, and observed at different times to growth of colonies. When these are evident (96 hours), the application of 2 Mi of the composition of the composition according to the invention at the concentrations mentioned above, in the form of an aerosol, to again observe the development of fungi and reapply in case of fungal growth.
Resultados Results
Tabla 6: Aplicación directa composición según la invención a concentración 3,8% p/vTable 6: Direct application composition according to the invention at a concentration of 3.8% w / v
Horas post Crecimiento post Post Hours Post Growth
Agente Fúngico Contaje de colonias aplicación aplicación Fungal Agent Colonies application application
Aspergillus niger Aspergillus niger
Sin incremento del Without increase of
Aspergillus flavus Aspergillus flavus
24 horas desarrollo de l( cfu/ml 24 hours development of l (cfu / ml
Ciado sporium spp. Ciado sporium spp.
colonias colonies
Mucor spp Mucor spp
Aspergillus niger Aspergillus niger
Sin incremento del Without increase of
Aspergillus flavus Aspergillus flavus
48 horas desarrollo de l( cfu/ml 48 hours development of l (cfu / ml
Ciado sporium spp. Ciado sporium spp.
colonias colonies
Mucor spp Mucor spp
Aspergillus niger Aspergillus niger
Sin incremento del Without increase of
Aspergillus flavus Aspergillus flavus
72 horas desarrollo de l( cfu/ml 72 hours development of l (cfu / ml
Ciado sporium spp. Ciado sporium spp.
colonias colonies
Mucor spp Mucor spp
Aspergillus niger Presenta Aspergillus niger Presents
Aspergillus flavus incremento del Aspergillus flavus increase of
Ciado sporium spp. desarrollo de Ciado sporium spp. development of
Mucor spp colonias con Mucor spp colonies with
144 horas crecimiento l( cfu/ml 144 hours growth l (cfu / ml
adicional, y se additional, and
reaplica la reapply
composición de la composition of
invención. invention.
Aspergillus niger Aspergillus niger
Sin incremento del Without increase of
24 horas (luego Aspergillus flavus 24 hours (after Aspergillus flavus
desarrollo de l( cfu/ml de reaplicación) Ciado sporium spp. development of l (cfu / ml reapplication) Ciado sporium spp.
colonias colonies
Mucor spp Tabla 7: Aplicación directa composición según la invención a concentración 38,4%p/vMucor spp Table 7: Direct application composition according to the invention at a concentration of 38.4% w / v
Horas post Post hours
Agente Fúngico Crecimiento Contaje de colonias aplicación Fungal Agent Growth Counting colonies application
Aspergillus niger Aspergillus niger
Sin incremento del Without increase of
Aspergillus flavus Aspergillus flavus
24 horas desarrollo de l( cfu/ml 24 hours development of l (cfu / ml
Ciado sporium spp. Ciado sporium spp.
colonias colonies
Mucor spp Mucor spp
Aspergillus niger Aspergillus niger
Sin incremento del Without increase of
Aspergillus flavus Aspergillus flavus
48 horas desarrollo de l( cfu/ml 48 hours development of l (cfu / ml
Ciado sporium spp. Ciado sporium spp.
colonias colonies
Mucor spp Mucor spp
Aspergillus niger Aspergillus niger
Sin incremento del Without increase of
Aspergillus flavus Aspergillus flavus
72 horas desarrollo de l( cfu/ml 72 hours development of l (cfu / ml
Ciado sporium spp. Ciado sporium spp.
colonias colonies
Mucor spp Mucor spp
Aspergillus niger Presenta Aspergillus niger Presents
Aspergillus flavus incremento del Aspergillus flavus increase of
Ciado sporium spp. desarrollo de Ciado sporium spp. development of
144 horas colonias con l( cfu/ml 144 hours colonies with l (cfu / ml
Mucor spp Mucor spp
crecimiento increase
adicional, y se additional, and
reaplica reapplies
Inaplicación Inapplication
Aspergillus niger Sin incremento del Aspergillus niger No increase in
24 horas (Luego de 24 hours (After
desarrollo de l( cfu/ml Reaplicación) development of l (cfu / ml Re-application)
colonias colonies
Tabla 8: Aplicación a paredes composición según la invención a concentración 3,8%p/v Table 8: Application to walls composition according to the invention at a concentration of 3.8% w / v
120 horas post aplicación a Aspergillus niger 120 hours post application to Aspergillus niger
paredes de envase y 48 horas Aspergillus flavus Sin incremento del desarrollo post aspersión directa a Ciado sporium spp de colonias container walls and 48 hours Aspergillus flavus No increase in direct post spray development to Ciado sporium spp.
lamina Mucor spp Mucor spp sheet
144 horas post aplicación a Aspergillus niger 144 hours post application to Aspergillus niger
paredes de envase y 72 horas Aspergillus flavus Sin incremento del desarrollo post aspersión directa a Ciado sporium spp de colonias container walls and 72 hours Aspergillus flavus No increase in direct post spray development to Ciado sporium spp.
lamina Mucor spp Mucor spp sheet
168 horas post aplicación a Aspergillus niger 168 hours post application to Aspergillus niger
paredes de envase y 96 horas Se observa crecimiento post aspersión directa a adicional de colonias lámina Container walls and 96 hours Post direct spraying growth to additional colony foil is observed
Tabla 9: Aplicación a paredes composición según la invención a concentración 38,4% p/v Table 9: Application to walls composition according to the invention at a concentration of 38.4% w / v
post aspersión directa a Ciado sporium spp post direct spraying to Ciado sporium spp
lamina Mucor spp Mucor spp sheet
168 horas post aplicación a Aspergillus niger 168 hours post application to Aspergillus niger
paredes de envase y 96 horas Se observa crecimiento post aspersión directa a adicional de colonias lamina Container walls and 96 hours Post-spraying direct growth to additional colony laminae is observed
Conclusiones del estudio Conclusions of the study
• La composición según la invención resulta ser eficaz para el control de crecimiento de hongos. • The composition according to the invention proves to be effective for the control of fungal growth.
• La composición según la invención en alta concentración (15,2% p/v) resulta ser efectivo como fungicida en las condiciones ensayadas. • The composition according to the invention in high concentration (15.2% w / v) is effective as a fungicide under the conditions tested.
• No se observan diferencias entre altas y bajas concentraciones de la composición según la invención, cuando ésta se aplica directamente sobre los cultivos en láminas. • No differences are observed between high and low concentrations of the composition according to the invention, when it is applied directly on the sheet cultures.
• No se observan diferencias entre altas y bajas concentraciones de la composición según la invención cuando ésta se aplica sobre las paredes internas del envase y posteriormente cuando se aplica directamente sobre los cultivos por desarrollo de éstos. • No differences are observed between high and low concentrations of the composition according to the invention when it is applied on the internal walls of the container and later when applied directly on the crops by their development.
• Para las concentraciones de hongos estudiadas se requieren dosis repetidas de la composición según la invención, cada 72 horas. • For the concentrations of fungi studied, repeated doses of the composition according to the invention are required every 72 hours.
• El uso de la composición según la invención en las paredes internas de los Envirochek retarda el desarrollo de los hongos estudiados y el refuerzo de aplicaciones de la composición se presenta en forma contante a partir de las 72 horas. • The use of the composition according to the invention in the internal walls of the Envirochek retards the development of the fungi studied and the reinforcement of applications of the composition occurs in constant form after 72 hours.
• Estas concentraciones ensayadas in vitro, son efectivas y suficientes para no afectar el ADN de las muestras (Moreno & McCord, 2017). Sin embargo, no se sugiere superar estas concentraciones. • These concentrations tested in vitro, are effective and sufficient to not affect the DNA of the samples (Moreno & McCord, 2017). However, it is not suggested to exceed these concentrations.
Ejemplo 2: Determinación de la efectividad como fungicida la composición de según la presente invención Example 2: Determination of the effectiveness as a fungicide of the composition according to the present invention
Para este estudio se empleó la siguiente composición: Compuesto (%) For this study, the following composition was used: Compound (%)
(NH4)2Cu(S04)2x6H20 36 (NH 4 ) 2 Cu (S0 4 ) 2 x6H 2 0 36
CuS04x3H20 25 CuS0 4 x3H 2 0 25
CuS04x5H20 15 CuS0 4 x5H 2 0 15
Cui5(S04)4(OH)22x6H20 < 1 Cui 5 (S0 4 ) 4 (OH) 22 x6H 2 0 <1
Cu3(S04)(OH)4 10 Cu 3 (S0 4 ) (OH) 4 10
CuS04(H20)3 < 1 CuS0 4 (H 2 0) 3 <1
Material Amorfo 12 Amorphous Material 12
Los ensayos se realizaron bajo condiciones controladas que intentan simular la aplicación del compuesto en forma de aerosol sobre material biológico contaminado con agentes fúngicos. Condiciones de los ensayos Material biológico blanco: conidios de Penicillium sp. (correspondiente al agente fúngico más representado en el muestreo de la dependencia identificada como "zona de restos óseos" del Departamento de Medicina Legal de la Facultad de Medicina, Universidad de Chile). Medio de cultivo: agar Sabouraud. The tests were carried out under controlled conditions that attempt to simulate the application of the compound in aerosol form on biological material contaminated with fungal agents. Test conditions White biological material: conidia of Penicillium sp. (corresponding to the fungal agent most represented in the sample of the unit identified as "bone remains area" of the Department of Legal Medicine of the Faculty of Medicine, University of Chile). Culture medium: Sabouraud agar.
Inoculo blanco: cada placa de medio fue inoculado con 195.000 conidios, en consonancia con la alta carga fúngica reportada. White inoculum: each plate of medium was inoculated with 195,000 conidia, in line with the high reported fungal load.
Tratamiento: cada ensayo se realizó en una cámara cerrada, con un volumen de 24 Litros, conteniendo tres placas de agar Sabouraud, previamente inoculadas con los conidios de Penicillium sp. Los ensayos consistieron en la aplicación en forma de aerosol, de un determinado volumen de una solución diluida de la composición a diferentes concentraciones. En la Tabla 10 se especifican los detalles de cada ensayo. Treatment: each test was carried out in a closed chamber, with a volume of 24 liters, containing three plates of Sabouraud agar, previously inoculated with the conidia of Penicillium sp. The tests consisted of the application in aerosol form of a certain volume of a diluted solution of the composition at different concentrations. Table 10 details the details of each trial.
Lectura de resultados: los efectos para cada tratamiento fueron evaluados después de tres días de incubación a temperatura ambiente. Para cada ensayo se determinó el área de crecimiento de los cultivos. Los resultados se expresan como el porcentaje de inhibición del crecimiento respecto al control sin tratar. Tabla 10: Condiciones de cada ensayo de inhibición del crecimiento con la composición de la invención. N° Ensayo1 Composición (%) Volumen Aplicado (mi) Reading of results: the effects for each treatment were evaluated after three days of incubation at room temperature. For each test, the growth area of the cultures was determined. The results are expressed as the percentage of inhibition of growth relative to the untreated control. Table 10: Conditions of each growth inhibition test with the composition of the invention. No. Essay 1 Composition (%) Volume Applied (mi)
Control 0 0 Control 0 0
Ensayo 1 1,0 5,0 Test 1 1.0 5.0
Ensayo 2 1,0 10,0 Test 2 1.0 10.0
Ensayo 3 2,0 5,0 Test 3 2.0 5.0
Ensayo 4 4,0 2,0 Test 4 4.0 2.0
Ensayo 5 4,0 5,0 Test 5 4.0 5.0
Los resultados mostraron que la aplicación de 5 mi (cada aplicación fue de 1 mi en forma de aerosol) de la composición de la presente invención al 1,0% p/v tuvo un efecto nulo sobre el crecimiento de Penicillium sp., mientras que la aplicación de 5 mi de la composición al 4,0%p/v redujo en un 98% el crecimiento del mismo hongo (ver Figura 4). The results showed that the application of 5 ml (each application was 1 ml in aerosol form) of the composition of the present invention at 1.0% w / v had a null effect on the growth of Penicillium sp., While the application of 5 ml of the composition at 4.0% w / v reduced the growth of the same fungus by 98% (see Figure 4).
Conclusiones del estudio Conclusions of the study
El uso de un volumen de 5 mi la composición de la invención al 2,0% podría ser suficiente para limitar el crecimiento fúngico en las condiciones que se realizaron los ensayos. The use of a volume of 5 ml the composition of the invention at 2.0% could be sufficient to limit the fungal growth under the conditions that the tests were carried out.
CONCLUSIONES GENERALES GENERAL CONCLUSIONS
La composición según la invención entrega una solución eficaz para el control de hongos y bacterias en osamentas y anatomía patológica con dosis suficientemente altas como controlar eficazmente hongos y bacterias, y al mismo tiempo lo suficientemente bajas como para no presentar efectos en el ADN del material descontaminado, lo que se debe particularmente a la eficiencia de la composición de la invención. The composition according to the invention provides an effective solution for the control of fungi and bacteria in bones and pathological anatomy with doses high enough to effectively control fungi and bacteria, and at the same time low enough so as not to have effects on the DNA of the decontaminated material. , which is particularly due to the efficiency of the composition of the invention.
Asimismo, la composición desarrollada permite su uso en la desinfección y sanitización industrial mediante efecto fungicida y bactericida en salas de proceso, salas de packing de fruta, fábricas, galpones, etc. Also, the composition developed allows its use in disinfection and industrial sanitization through fungicide and bactericidal effect in process rooms, fruit packing rooms, factories, warehouses, etc.
La aplicación de la composición de la presente invención provee una solución efectiva en, por ejemplo, desinfección, descontaminación y sanitización mediante efecto fungicida bactericida en manejo de catástrofes, hospitales, industria ganadera, industria salmonera, industria agropecuaria en general y para la industria alimentaria. The application of the composition of the present invention provides an effective solution in, for example, disinfection, decontamination and sanitization by bactericidal fungicidal effect in catastrophe management, hospitals, livestock industry, salmon industry, agricultural industry in general and for the food industry.
La especificación precedente se proporciona solo para propósitos ilustrativos y no es destinada a describir todos los aspectos posibles de la presente invención. Mientras la invención se ha mostrado en el presente documento y se han descrito detalladamente con respecto a varias modalidades ejemplares, los expertos en la técnica apreciarán que cambios menores a la descripción y varias otras modificaciones, omisiones y adiciones se hacen sin apartarse del espíritu y el alcance de los mismos. The foregoing specification is provided for illustrative purposes only and is not intended to describe all possible aspects of the present invention. While the invention has been shown in the present document and have been described in detail with respect to several exemplary embodiments, those skilled in the art will appreciate that minor changes to the description and various other modifications, omissions and additions are made without departing from the spirit and scope of them.
Claims
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762577110P | 2017-10-25 | 2017-10-25 | |
| US62/577,110 | 2017-10-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2019079912A1 true WO2019079912A1 (en) | 2019-05-02 |
Family
ID=66247088
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CL2018/050103 Ceased WO2019079912A1 (en) | 2017-10-25 | 2018-10-25 | Method for controlling fungi and bacteria in bones and in the field of anatomical pathology; method for disinfecting and sanitising contaminated areas and/or surfaces; and uses |
| PCT/CL2018/050102 Ceased WO2019079911A1 (en) | 2017-10-25 | 2018-10-25 | Fungicidal and bactericidal composition comprising copper-based salts; method for preventing and/or treating the infestation of useful plants with bacteria or fungi; and use of said composition |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CL2018/050102 Ceased WO2019079911A1 (en) | 2017-10-25 | 2018-10-25 | Fungicidal and bactericidal composition comprising copper-based salts; method for preventing and/or treating the infestation of useful plants with bacteria or fungi; and use of said composition |
Country Status (3)
| Country | Link |
|---|---|
| AR (1) | AR113795A1 (en) |
| PE (1) | PE20210476A1 (en) |
| WO (2) | WO2019079912A1 (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060178431A1 (en) * | 2004-12-21 | 2006-08-10 | Hartfeldt Will H | Chemotherapeutic agents for the control of plant and animal diseases |
| CL2013002173A1 (en) * | 2013-07-30 | 2014-02-21 | Compania Minera San Geronimo | Agrochemical formulation comprising soluble copper pentahydrate sulfate, cuso4 x 5h2o and insoluble alkaline copper sulfate; obtaining process; and its use for the treatment and prevention of diseases caused by fungi and bacteria. |
| US8951573B2 (en) * | 2010-05-21 | 2015-02-10 | Lion Corporation | Disinfectant composition for hard articles, and method for disinfecting of surface of hard article |
| MX2015009708A (en) * | 2013-02-01 | 2016-09-13 | Compañia Minera San Geronimo | Impregnatable matrix of plant, animal or synthetic origin or mixtures of same, containing a uniformly distributed antimicrobial compound, method for impregnating said matrix with a compound, and use thereof in the production of antimicrobial elements. |
| US9474282B2 (en) * | 2013-12-13 | 2016-10-25 | Tony John Hall | Acid-solubilized copper-ammonium complexes and copper-zinc-ammonium complexes, compositions, preparations, methods, and uses |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6923991B2 (en) * | 2002-02-20 | 2005-08-02 | Global Microbio Products, L.L.C. | Bactericide solution and method of making same |
| US20170055534A1 (en) * | 2015-08-25 | 2017-03-02 | Phyton Corporation | Process for treating plants to control bacterial and fungal growth |
-
2018
- 2018-10-25 WO PCT/CL2018/050103 patent/WO2019079912A1/en not_active Ceased
- 2018-10-25 PE PE2020000431A patent/PE20210476A1/en unknown
- 2018-10-25 WO PCT/CL2018/050102 patent/WO2019079911A1/en not_active Ceased
- 2018-10-25 AR ARP180103110A patent/AR113795A1/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060178431A1 (en) * | 2004-12-21 | 2006-08-10 | Hartfeldt Will H | Chemotherapeutic agents for the control of plant and animal diseases |
| US8951573B2 (en) * | 2010-05-21 | 2015-02-10 | Lion Corporation | Disinfectant composition for hard articles, and method for disinfecting of surface of hard article |
| MX2015009708A (en) * | 2013-02-01 | 2016-09-13 | Compañia Minera San Geronimo | Impregnatable matrix of plant, animal or synthetic origin or mixtures of same, containing a uniformly distributed antimicrobial compound, method for impregnating said matrix with a compound, and use thereof in the production of antimicrobial elements. |
| CL2013002173A1 (en) * | 2013-07-30 | 2014-02-21 | Compania Minera San Geronimo | Agrochemical formulation comprising soluble copper pentahydrate sulfate, cuso4 x 5h2o and insoluble alkaline copper sulfate; obtaining process; and its use for the treatment and prevention of diseases caused by fungi and bacteria. |
| US9474282B2 (en) * | 2013-12-13 | 2016-10-25 | Tony John Hall | Acid-solubilized copper-ammonium complexes and copper-zinc-ammonium complexes, compositions, preparations, methods, and uses |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019079911A1 (en) | 2019-05-02 |
| PE20210476A1 (en) | 2021-03-08 |
| AR113795A1 (en) | 2020-06-10 |
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