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WO2018129486A3 - Composition et procédés pour une expression améliorée de gène rapporteur knock-in - Google Patents

Composition et procédés pour une expression améliorée de gène rapporteur knock-in Download PDF

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Publication number
WO2018129486A3
WO2018129486A3 PCT/US2018/012849 US2018012849W WO2018129486A3 WO 2018129486 A3 WO2018129486 A3 WO 2018129486A3 US 2018012849 W US2018012849 W US 2018012849W WO 2018129486 A3 WO2018129486 A3 WO 2018129486A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
composition
gene expression
reporter gene
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2018/012849
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English (en)
Other versions
WO2018129486A2 (fr
Inventor
Tim D. Ahfeldt
Lee L. Rubin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of WO2018129486A2 publication Critical patent/WO2018129486A2/fr
Publication of WO2018129486A3 publication Critical patent/WO2018129486A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des procédés, des vecteurs, des cellules transgéniques et des compositions pour exprimer un marqueur avec un gène d'intérêt. En particulier, l'invention concerne des procédés, des vecteurs, des cellules transgéniques et des compositions pour une expression élevée d'une protéine fluorescente telle que TDTomato avec un gène d'intérêt tel que la tyrosine hydroxylase afin d'évaluer l'expression du gène d'intérêt in vivo et in vitro.
PCT/US2018/012849 2017-01-06 2018-01-08 Composition et procédés pour une expression améliorée de gène rapporteur knock-in Ceased WO2018129486A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762443543P 2017-01-06 2017-01-06
US62/443,543 2017-01-06

Publications (2)

Publication Number Publication Date
WO2018129486A2 WO2018129486A2 (fr) 2018-07-12
WO2018129486A3 true WO2018129486A3 (fr) 2018-11-15

Family

ID=62790908

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2018/012849 Ceased WO2018129486A2 (fr) 2017-01-06 2018-01-08 Composition et procédés pour une expression améliorée de gène rapporteur knock-in

Country Status (1)

Country Link
WO (1) WO2018129486A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190127713A1 (en) 2016-04-13 2019-05-02 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
WO2018129486A2 (fr) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition et procédés pour une expression améliorée de gène rapporteur knock-in
US12509492B2 (en) 2018-01-19 2025-12-30 Duke University Genome engineering with CRISPR-Cas systems in eukaryotes
EP4010485A4 (fr) * 2019-08-08 2023-08-23 Duke University Plate-forme de criblage à haut débit pour l'ingénierie de vecteurs de thérapie génique de nouvelle génération

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136597A (en) * 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
WO2015089419A2 (fr) * 2013-12-12 2015-06-18 The Broad Institute Inc. Délivrance, utilisation et applications thérapeutiques des systèmes crispr-cas et compositions permettant de cibler des troubles et maladies au moyen de constituants de délivrance sous forme de particules
US20160186213A1 (en) * 2013-06-17 2016-06-30 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
WO2018129486A2 (fr) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition et procédés pour une expression améliorée de gène rapporteur knock-in

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136597A (en) * 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
US20160186213A1 (en) * 2013-06-17 2016-06-30 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
WO2015089419A2 (fr) * 2013-12-12 2015-06-18 The Broad Institute Inc. Délivrance, utilisation et applications thérapeutiques des systèmes crispr-cas et compositions permettant de cibler des troubles et maladies au moyen de constituants de délivrance sous forme de particules
WO2018129486A2 (fr) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition et procédés pour une expression améliorée de gène rapporteur knock-in

Also Published As

Publication number Publication date
WO2018129486A2 (fr) 2018-07-12

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