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WO2018129486A3 - Composition and methods for enhanced knock-in reporter gene expression - Google Patents

Composition and methods for enhanced knock-in reporter gene expression Download PDF

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Publication number
WO2018129486A3
WO2018129486A3 PCT/US2018/012849 US2018012849W WO2018129486A3 WO 2018129486 A3 WO2018129486 A3 WO 2018129486A3 US 2018012849 W US2018012849 W US 2018012849W WO 2018129486 A3 WO2018129486 A3 WO 2018129486A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
composition
gene expression
reporter gene
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2018/012849
Other languages
French (fr)
Other versions
WO2018129486A2 (en
Inventor
Tim D. Ahfeldt
Lee L. Rubin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Publication of WO2018129486A2 publication Critical patent/WO2018129486A2/en
Publication of WO2018129486A3 publication Critical patent/WO2018129486A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Provided are methods, vectors, transgenic cells, and compositions for expressing a marker with a gene of interest. In particular, provided are methods, vectors, transgenic cells and compositions for high expression of a fluorescent protein such as TDTomato with a gene of interest such as tyrosine hydroxylase in order to assess the expression of the gene of interest in vivo and in vitro.
PCT/US2018/012849 2017-01-06 2018-01-08 Composition and methods for enhanced knock-in reporter gene expression Ceased WO2018129486A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762443543P 2017-01-06 2017-01-06
US62/443,543 2017-01-06

Publications (2)

Publication Number Publication Date
WO2018129486A2 WO2018129486A2 (en) 2018-07-12
WO2018129486A3 true WO2018129486A3 (en) 2018-11-15

Family

ID=62790908

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2018/012849 Ceased WO2018129486A2 (en) 2017-01-06 2018-01-08 Composition and methods for enhanced knock-in reporter gene expression

Country Status (1)

Country Link
WO (1) WO2018129486A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190127713A1 (en) 2016-04-13 2019-05-02 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
WO2018129486A2 (en) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition and methods for enhanced knock-in reporter gene expression
US12509492B2 (en) 2018-01-19 2025-12-30 Duke University Genome engineering with CRISPR-Cas systems in eukaryotes
EP4010485A4 (en) * 2019-08-08 2023-08-23 Duke University HIGH-THROUGHPUT SCREENING PLATFORM FOR THE MANIPULATION OF NEXT GENERATION GENE THERAPY VECTORS

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136597A (en) * 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
WO2015089419A2 (en) * 2013-12-12 2015-06-18 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
US20160186213A1 (en) * 2013-06-17 2016-06-30 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
WO2018129486A2 (en) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition and methods for enhanced knock-in reporter gene expression

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136597A (en) * 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
US20160186213A1 (en) * 2013-06-17 2016-06-30 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
WO2015089419A2 (en) * 2013-12-12 2015-06-18 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
WO2018129486A2 (en) * 2017-01-06 2018-07-12 President And Fellows Of Harvard College Composition and methods for enhanced knock-in reporter gene expression

Also Published As

Publication number Publication date
WO2018129486A2 (en) 2018-07-12

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