WO2018074640A1 - Pharmaceutical composition, health functional food, and cosmetic composition for preventing or treating hair loss - Google Patents

Abstract

The present invention relates to a pharmaceutical composition, a health functional food, and a cosmetic composition for preventing or treating hair loss and, specifically, to a pharmaceutical composition, a health functional food, and a cosmetic composition for preventing or treating hair loss, which comprise a Houttuyniae Herba extract, a Perillae Herba extract, and a green tea extract, wherein the pharmaceutical composition, health functional food, and cosmetic composition comprise 25-75 parts by weight of a Perillae Herba extract and 25-75 parts by weight of a green tea extract relative to 100 parts by weight of a Houttuyniae Herba extract, and thus can exhibit an excellent hair loss prevention, relief, or treatment effect through a synergistic effect thereof.

Description

Pharmaceutical composition, nutraceutical and cosmetic composition for preventing or treating hair loss

The present invention relates to a pharmaceutical composition and nutraceutical for preventing or treating hair loss.

Hair is one of the major parts of the body derived from the outer cell layer of the skin and is considered an appendage of the skin along with sebaceous glands, sweat glands and nails. Hair is also known as skin inducer because it derives from the skin during developmental growth. Hair is important for the overall appearance of the human body, and despite the fact that some medical treatments have been developed, many people suffer from hair loss or hair weakness.

The causes of hair loss include excessive male hormone action, excessive sebaceous secretion, poor blood circulation, poor peroxide, bacteria, deterioration of scalp function, hypoglycemic collagen production, genetic factors, aging and stress. come. However, to date, no clear cause for hair loss is known, and in recent years, the hair loss population is increasing due to changes in dietary life and social stress, and the age is also decreasing and the female hair loss population is increasing.

Conventionally, there are methods for promoting blood circulation, strengthening hair root function, and scalp moisturizing to treat or prevent hair loss, and commercially available medicines are mainly made of female hormones for suppressing male hormones or high blood pressure drugs that control blood pressure. Minoxidil, which is developed to focus on the side effects of hair growth, is sold to patients, but there are side effects such as loss of energy, and there is a disadvantage that if the use is stopped, the effect does not persist and returns to the state before use. It is true.

Therefore, it is urgent to develop a therapeutic method and a pharmaceutical composition for preventing hair loss, hair growth and hair growth by using ingredients derived from natural materials and having no side effects.

Korean Patent No. 10-0800359 discloses a composition exhibiting hair loss prevention and hair growth promoting effect.

An object of the present invention is to provide a pharmaceutical composition for preventing or treating hair loss derived from natural materials.

An object of the present invention is to provide a health functional food for preventing or improving hair loss derived from natural materials.

1. Containing Echo extract, leaf leaf extract and green tea extract,

Pharmaceutical composition for the prevention or treatment of hair loss comprising 25 to 75 parts by weight of Japonica leaf extract and 25 to 75 parts by weight of green tea extract based on 100 parts by weight of the Echochocho extract.

2. according to the above 1, the Echochocho extract, Japonica extract and green tea extract are each independently extracted and mixed, pharmaceutical composition for the prevention or treatment of hair loss.

3. according to the above 1, the Echochocho extract, leaf extract and green tea extract is not fermented, the pharmaceutical composition for the prevention or treatment of hair loss.

4. according to the above 1, black bean extract, glutinous rice extract, kelp extract, cheongung extract, nasturtium extract, root extract and licorice extract further comprises one or more extracts selected from the group, the pharmaceutical composition for the prevention or treatment of hair loss.

5. Includes Echo extract, Japonica extract and Green tea extract,

Health functional food for the prevention or improvement of hair loss comprising 25 to 75 parts by weight of the extract of Japonica leaves and 25 to 75 parts by weight of green tea extract with respect to 100 parts by weight of the fish extract.

6. In the above 5, the Echochocho extract, Japonica extract and green tea extract are each independently extracted and mixed, health functional food for the prevention or improvement of hair loss.

7. In the above 5, wherein the Echochocho extract, leaf extract and green tea extract is not fermented, health functional food for the prevention or improvement of hair loss.

8. In the above 5, the black bean extract, glutinous rice extract, kelp extract, cheongung extract, nasturtium extract, root extract and licorice extract further comprises one or more extracts selected from the group, hair loss prevention or functional food .

9. Includes Echo extract, Fructus leaf extract and Green tea extract,

Cosmetic composition for the prevention or improvement of hair loss comprising 25 to 75 parts by weight of the extract of Japonica leaf and 25 to 75 parts by weight of green tea extract with respect to 100 parts by weight of the Echochocho extract.

10. In the above 9, the Echochocho extract, cotyledon extract and green tea extract are each independently extracted and mixed, cosmetic composition for the prevention or improvement of hair loss.

11. In the above 9, wherein the Echochocho extract, leaf extract and green tea extract is not fermented, cosmetic composition for the prevention or improvement of hair loss.

12. In the above 9, black soybean extract, glutinous rice extract, kelp extract, cheongung extract, nasturtium extract, root extract and licorice extract further comprises one or more extracts selected from the group, cosmetic composition for preventing or improving hair loss.

The pharmaceutical composition, nutraceutical and cosmetic composition for preventing or treating hair loss according to the present invention exhibit a very excellent hair loss prevention, improvement or treatment effect.

1 shows the results of visual observation of the state of rat hair.

Figure 2 shows the results of H & E staining of rat skin tissue.

Figure 3 shows the expression of IGF-1 and TGF-β1 in rat skin tissue.

Figure 4 shows the amount of IGF-1 and TGF-β1 expression in rat skin tissue.

The present invention relates to a pharmaceutical composition, health functional food and cosmetic composition for the prevention or treatment of hair loss, including Echochocho extract, Japonica leaf extract and Green tea extract, 25-75% Japonica leaf extract based on 100 parts by weight of the Echochocho extract. By including 25 to 75 parts by weight of the green tea extract, and synergistic effects thereof, the pharmaceutical composition, health functional food and cosmetic composition for the prevention or treatment of hair loss, which can exhibit a very good hair loss prevention, improvement or treatment effect .

Hereinafter, the present invention will be described in detail.

The pharmaceutical composition for preventing or treating hair loss according to the present invention includes Echochocho extract, Japonica leaf extract and Green tea extract, and 25-75 parts by weight of Japonica leaf extract and 25-75 parts by weight of green tea extract based on 100 parts by weight of Echochocho extract. Include.

* Fish herb has antibacterial and anti-inflammatory effect, and has excellent antibacterial effect against hair loss bacteria such as Malassezia and Staphylococcus.

The leaflets are rich in minerals, vitamins B1, B2, B6, vitamin C, vitamin E, vitamin K and niacin, and are rich in calcium, potassium, iron, magnesium, zinc, and other alphalinolenes, which break down cholesterol in the body. It contains almost all of the coenzyme components needed by the human body. In addition, perylaldehyde and beta-carotene exhibit strong antibacterial and antioxidant activity, and when used together with fish herb, shows excellent synergistic effect on hair loss.

Green tea is an evergreen tree or shrub belonging to theaceae. Polyphenols found in green tea are known as flavonols and catechins, and are known to have various effects such as antioxidant, anticancer, anti-inflammatory, and antibacterial effects, and promote hair growth and hair root strengthening.

According to the present invention, if the mixing ratio of Echochocho extract, Japonica leaf extract and green tea extract is within the above range, the hair loss prevention or treatment effect may be excellent.

In addition, in terms of maximizing the synergistic effect of Echochocho, Japonica and Green Tea, the extract may include 55-75% by weight of Japonica Leaf Extract and 55-75% by weight of Green Tea Extract, and more preferably It may be included as 60 to 75 parts by weight of the extract of Japonica leaves and 60 to 75 parts by weight of green tea extract based on 100 parts by weight of Eochocho extract. For example, Echochocho extract, cotyledon extract and green tea extract may be included as 70 parts by weight of Japonica leaf extract and 70 parts by weight of green tea extract based on 100 parts by weight of Echocho extract.

The extracts of the medicinal herbs may be prepared according to a conventional method of producing a plant extract, for example, by adding an extracting solvent to the flowers, leaves, branches, roots, berries or shells or pulverized products of the components of each plant. Alternatively, the extract may be prepared by adding a fractional solvent to the crude extract prepared by extraction with an extraction solvent.

The extraction solvent may be at least one selected from the group consisting of water and an organic solvent. The organic solvent may be a polar solvent such as an alcohol having 1 to 5 carbon atoms, the alcohol dilution ethyl acetate or acetone and a nonpolar solvent of ether, chloroform, benzene, hexane or dichloromethane, or a mixed solvent thereof. The alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol, isopropanol, and the like, but is not limited thereto. In addition, the alcohol dilution water may be one obtained by diluting the alcohol in water at 50 to 99.9% (v / v).

Extraction solvent used to obtain the extract of the present invention is preferably at least one selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, alcohol dilution water and mixtures thereof, more preferably water, 1 carbon atoms It may be any one selected from the group consisting of alcohols of 4 to 4 and mixtures thereof, and most preferably 30 to 99% aqueous ethanol or alcohol. For example, the extraction process may be performed at 4 ° C. to 120 ° C., or 50 ° C. to 90 ° C., or 70 ° C. to 85 ° C., but is not limited thereto. In addition, the extraction time is not particularly limited, but may be 10 minutes to 12 hours, preferably 1 hour to 6 hours.

In addition, specific extraction methods include cold extraction method, hot extraction method, thermal extraction method, ultrasonic extraction method, and the like, it is possible to use a conventional extraction device, ultrasonic grinding extractor or fractionator.

In addition, the extract extracted with the solvent may be further subjected to the fractionation process with any one solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water and mixtures thereof. Two or more solvents may be used in the fractionation, and each solvent extract may be prepared using sequentially or mixed according to the polarity of the solvent. In addition, the fractionation process may be performed at 4 ° C. to 120 ° C., or 50 ° C. to 90 ° C., or 70 ° C. to 85 ° C., but is not limited thereto.

The prepared extract or the fraction obtained by performing the fractionation process can be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a reduced pressure filter, the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

According to one embodiment of the invention, Eochocho extract, cotyledon extract, and green tea extract may be each independently extracted and mixed.

The medicinal herbs are independently extracted and then mixed may exhibit a better hair loss prevention and treatment effect than simultaneously extracting Echoseong, cotyledon and green tea together.

According to one embodiment of the present invention, Eoseongcho extract, cotyledon extract and green tea extract may be unfermented. Therefore, a problem in process control such as corruption may not occur, and the process may be simple to enable manufacturing in a short time.

 According to an embodiment of the present invention, the pharmaceutical composition for preventing or treating hair loss may further include a Angelica extract, Salvia extract and Gardenia extract, but the content ratio is not limited, Angelica extract 50 compared to 100 parts by weight of Echochocho extract To 100 parts by weight, 25 to 75 parts by weight of Salvia extract and 15 to 50 parts by weight of gardenia extract.

Dongguk is a word that takes the root of Shingamchae from one shot and improves the blood circulation of the scalp.

Salvia is a medicinal herb made from the roots of Salvia miltiorrhiza Bunge (Korea and China). This helps with blood circulation, removes blood and relieves limb pain. Menstrual dysmenorrhea, menstrual pain, postpartum abdominal pain, bloody heart abdominal pain, bruises, etc. are treated. Heals mental fever, bullshit, annoyance, insomnia, skin rash, palpitations due to high fever. Pharmacological action has been reported to coronary dilatation, lower cholesterol, lower blood pressure, activate liver function, sedative anti-inflammatory, anticancer, antibacterial activity.

Gardenia is a fruit of gardenia and related plants, including gardenin, pectin, tannin, crocin, gardenoside, geniposide, scandoside (scandoside) and other ingredients are known, the effect on the ginger, blood pressure lowering action has been known.

According to another embodiment of the present invention, the pharmaceutical composition for preventing or treating hair loss further comprises one or more extracts selected from the group consisting of black bean extract, glutinous rice extract, kelp extract, celery extract, nasturtium extract, brown root extract, and licorice extract. It may include.

Each of the medicinal extracts may be extracted according to the method described above.

Each of the medicinal extracts may further increase the effect of preventing or improving hair loss.

The pharmaceutical compositions of the present invention can be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral dosage forms, external preparations, suppositories, and sterile injectable solutions according to conventional methods.

Carriers, excipients and diluents that may be included in the composition containing the extract of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber , Alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil have. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used.

Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose or lactose in the compound. Mixed with gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The amount of the pharmaceutical composition of the present invention may vary depending on the age, sex, and weight of the patient, but may be 0.1 to 100 mg / kg, preferably 1 to 10 mg / kg once or several times daily. The dosage may also be increased or decreased depending on the route of administration, the severity of the disease, sex, weight, age, and the like. Therefore, the above dosage does not limit the scope of the present invention in any aspect.

The pharmaceutical composition of the present invention can be used for female hair loss, circular hair loss, such as O-shaped hair loss, M-shaped hair loss, OM complex hair loss, lateral hair loss, hereditary hair loss, male hair loss, diffuse hair loss in which the hair at the crown area becomes thinner and thinner, and the like. It can be used for various hair loss symptoms.

Health functional foods for preventing or improving hair loss according to the present invention includes Echochocho extract, Japonica leaf extract and Green tea extract, 25-75 parts by weight of Japonica leaf extract and 25-75 parts by weight of green tea extract based on 100 parts by weight of Echochocho extract Include.

Each extract may be one extracted according to the method described above.

In accordance with the present invention, if the mixing ratio of Echochocho extract, Japonica extract and Green Tea extract is within the above range, it may be excellent in preventing hair loss or improving effect.

In addition, in terms of maximizing the synergistic effect of Echochocho, Japonica and Green Tea, the extract may include 55 to 75% by weight of Japonica Leaf Extract and 55 to 75% by weight of Green Tea Extract, and more preferably, 100 parts by weight of Echochocho extract. It may be included as 60 to 75 parts by weight of the extract of Japonica leaves and 60 to 75 parts by weight of green tea extract based on 100 parts by weight of Eochocho extract. For example, Echochocho extract, cotyledon extract and green tea extract may be included as 70 parts by weight of Japonica leaf extract and 70 parts by weight of green tea extract based on 100 parts by weight of Echocho extract.

According to one embodiment of the invention, Eochocho extract, cotyledon extract, and green tea extract may be each independently extracted and mixed.

The medicinal herbs are independently extracted and then mixed may exhibit a better hair loss prevention and improvement effect than simultaneously extracting Echoseong, cotyledon and green tea together.

According to one embodiment of the present invention, Eoseongcho extract, cotyledon extract and green tea extract may be unfermented. Therefore, a problem in process control such as corruption may not occur, and the process may be simple to enable manufacturing in a short time.

* According to one embodiment of the present invention, the health functional food for preventing or improving hair loss may further include a Angelica extract, Salvia extract and Gardenia extract, the content ratio is not limited, Angelica per 100 parts by weight extract It may be 50 to 100 parts by weight of the extract, 25 to 75 parts by weight of Salvia extract and 15 to 50 parts by weight of gardenia extract.

According to another embodiment of the present invention, it may further include one or more extracts selected from the group consisting of black soybean extract, glutinous rice extract, kelp extract, cheongung extract, nasturtium extract, brown root extract and licorice extract.

Each of the medicinal extracts may be extracted according to the method described above.

Each of the medicinal extracts may further increase the effect of preventing or improving hair loss.

Health functional food of the present invention is a beverage containing the extract (including alcoholic beverages), fruits and processed foods thereof (e.g. canned fruit, canned food, jam, marmalade, etc.), fish, meat and processed foods thereof (e.g. Hams, sausage corn, etc.), breads and noodles (e.g. udon, soba noodles, ramen noodles, spaghetti, macaroni, etc.), fruit juices, various drinks, cookies, syrups, dairy products (e.g. butter, cheese, etc.), edible vegetable oils, Margarine, vegetable protein, retort food, frozen food, various seasonings (eg, miso, soy sauce, sauce, etc.).

In addition, the health functional food of the present invention may be formulated into tablets, pills, powders, granules, powders, capsules, liquid formulations and the like. These may be formulated further comprising one or more of carriers, diluents, excipients and additives.

As additives that may be further included in the present invention, natural carbohydrates, flavors, nutrients, vitamins, minerals (electrolytes), flavors (synthetic flavors, natural flavors, etc.), colorants, fillers (cheese, chocolate, etc.), facts Acids and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, antioxidants, glycerin, alcohols, carbonating agents and flesh may be used.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used.

In addition to the above, the health functional food of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid And salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health functional food of the present invention may contain flesh for preparing natural fruit juice and vegetable beverage. These components can be used independently or in combination.

Specific examples of the carrier, excipient, diluent and additives include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, erythritol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium phosphate, calcium Silicates, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water, sugar syrup, methylcellulose, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate And at least one selected from the group consisting of mineral oils.

The content of the health functional food of the present invention as an active ingredient in the above-described formulations can be appropriately adjusted according to the use form and purpose, user condition, type of symptom and the weight, etc., 0.001 to 99.9% by weight, based on the solid content weight, preferably May be 0.01 to 50% by weight, but is not limited thereto.

In addition, the cosmetic composition for preventing or improving hair loss according to the present invention includes Echochocho extract, Japonica leaf extract and Green tea extract, 25-75 parts by weight of Japonica leaf extract and Green Tea extract 25-75 with respect to 100 parts by weight of the Echochocho extract It includes parts by weight.

Each extract may be one extracted according to the method described above.

In accordance with the present invention, if the mixing ratio of Echochocho extract, Japonica extract and Green Tea extract is within the above range, it may be excellent in preventing hair loss or improving effect.

In addition, in terms of maximizing the synergistic effect of Echochocho, Japonica and Green Tea, the extract may include 55 to 75% by weight of Japonica Leaf Extract and 55 to 75% by weight of Green Tea Extract, and more preferably, 100 parts by weight of Echochocho extract. It may be included as 60 to 75 parts by weight of the extract of Japonica leaves and 60 to 75 parts by weight of green tea extract based on 100 parts by weight of Eochocho extract. For example, Echochocho extract, cotyledon extract and green tea extract may be included as 70 parts by weight of Japonica leaf extract and 70 parts by weight of green tea extract based on 100 parts by weight of Echocho extract.

According to one embodiment of the invention, Eochocho extract, cotyledon extract, and green tea extract may be each independently extracted and mixed.

The medicinal herbs are independently extracted and then mixed may exhibit a better hair loss prevention and improvement effect than simultaneously extracting Echoseong, cotyledon and green tea together.

According to one embodiment of the present invention, Eoseongcho extract, cotyledon extract and green tea extract may be unfermented. Therefore, a problem in process control such as corruption may not occur, and the process may be simple to enable manufacturing in a short time.

According to one embodiment of the present invention, the cosmetic composition for preventing or improving hair loss may further include a Angelica extract, Salvia extract and Gardenia extract, the content ratio is not limited, Angelica extract 50 compared to 100 parts by weight Eochocho extract To 100 parts by weight, 25 to 75 parts by weight of Salvia extract and 15 to 50 parts by weight of gardenia extract.

According to another embodiment of the present invention, the cosmetic composition for preventing or improving hair loss is more than one extract selected from the group consisting of black soybean extract, glutinous rice extract, kelp extract, cheongung extract, nasturtium extract, brown root extract, licorice extract It may include.

Each of the medicinal extracts may be extracted according to the method described above.

Each of the medicinal extracts may further increase the effect of preventing or improving hair loss.

The cosmetic composition of the present invention may further include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts.

The water-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. And salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) can also be used in the present invention. Included in The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) and the like. Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl) Ethyl ether) and the like are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be incorporated into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be incorporated into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid and the like can be given. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic composition of the present invention may further include other components which are usually formulated into cosmetics as necessary in addition to the essential components.

Ingredients that can be formulated include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, and blood circulation accelerators. , A cooling agent, a restriction | limiting agent, purified water, etc. are mentioned.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearic acid Butyl acid, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octylate dodecyl, isostearic acid isetyl, diethyl sebacinate, adidi Diisopropyl phosphate, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopentane Tall, cetyl caprylate, lauric decyl laurate, hexyl laurate, myristicate decyl, myristic acid myristyl, myristicate cetyl, stearyl stearate, decyl oleate, risinooleate, Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2- Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid) propylene glycol, dicapryl Acid propylene glycol, dipentane neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitate glyceryl, triisostearic acid glyceryl neopentane dodecyl, Isostearyl octanoate, octyl isononate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Octyl stearate, polyglycerol oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, cetyl lactate, octyl lactate, triethyl citrate , Acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, diethyl ethyl succinate, diisobutyl adipicate, diisopropyl sebacate, seba Dioctyl Citrate, Cholesterol Stearate, Cholesterol Isostearate, Cholesterol Hydroxystearate, Cholesterol Oleate, Dihydrocholesteryl Oleate, Pitsteryl Isotearate, Pysterol Oleate, 12 -Steloylhydroxystearate isocetyl, 12-stealoylhydroxystearate stearyl, 12-stealoyl Ester system, such as hydroxystearic acid isostearyl, etc. are mentioned.

Hydrocarbon-based oils, such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystal wax, and petrolatum, are mentioned as hydrocarbon-based oils and fats.

As the silicone-based fats and oils, polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, Alkyl modified silicone oil, amino modified silicone oil, etc. are mentioned.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable fats and oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grapes. Seed oil, Cottonseed oil, Palm oil, Cuckoo nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba Animal or vegetable fats and oils, such as oil, candler wax, carnava wax, liquid lanolin, hardened castor oil, etc. are mentioned.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), poly Propylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

As the water-soluble polymer, carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, dextrin, etc. Can be mentioned.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl ester, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, an amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, laurin Acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphorus salts, alkyls Amide phosphate, alkyloylalkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate salt, sodium alkyl sulfo acetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. Can be.

Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium, behenyltrimethylammonium bromide, Benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type and the like. Amphoteric surfactants; and the like.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengal, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and oxide Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, coloramine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

Examples of the organic powder include metal soaps such as calcium stearate; Alkyl phosphate metal salts such as zinc sodium cetyl acid, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine Acyl basic amino acids; N-acylpolypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid, and cinnamic acid. Benzyl, paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, Urocanoic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzo Phenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy)- 1,3,5-triazine, 2- (2-hydroxy -5-methylphenyl) benzotriazole etc. are mentioned.

The fungicides include hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinphylthione, benzalkonium chloride, Photosensitizer 301, mononitrourecosodium, undecylenic acid, and the like.

Examples of the antioxidants include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

As alcohol, higher alcohols, such as cetyl alcohol, are mentioned.

In addition, the compounding component which may be added other than this is not limited to this, Although any said component can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01 to 5% weight percentage with respect to a total weight, More Preferably 0.01 to 3% by weight.

The cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Components included in the cosmetic composition of the present invention as an active ingredient may include components commonly used in cosmetic compositions in addition to the medicinal extract, for example, conventional stabilizers, solubilizers, vitamins, pigments and flavorings Adjuvants and carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nourishment cream, hair moisturizer cream, hair massage cream, hair wax, Hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drier, hair preservative, hair dye, hair wave agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer It may be prepared as a cosmetic, such as a riser, hair mousse or hair spray, but is not limited thereto.

The pharmaceutical composition of the present invention can be used for female hair loss, circular hair loss, such as O-shaped hair loss, M-shaped hair loss, OM complex hair loss, lateral hair loss, hereditary hair loss, male hair loss, diffuse hair loss in which the hair at the crown area becomes thinner and thinner, and the like. It can be used for various hair loss symptoms.

Hereinafter, the present invention will be described in detail with reference to Examples.

Example

In vitro experiment

1. Experimental Materials

1 g of edible vinegar was added to 15 ml of ethanol 30%, extracted at 86 ° C. for 3 hours, and concentrated by filtration, hot air drying or spray drying to prepare a eosung vinegar extract.

1 g of leaflets in 15 ml of ethanol was extracted and extracted at 86 ° C. for 3 hours, filtered and concentrated by hot air drying or spray drying to prepare a leaf extract.

1 g of green tea was added to 15 ml of ethanol 30%, extracted at 86 ° C. for 3 hours, and concentrated by filtration, hot air drying or spray drying to prepare green tea extract.

The extract was mixed in the content ratio shown in Table 1 (mixed after extraction).

In addition, Examples 5 to 10 of Table 2 were made to compare the effects of the effect of the fermentation and extraction method and the effect of the Example.

In Example 5, after extracting 1 g of eosungcho, 1 g of Japonica leaf, and 1 g of green tea at 86 ° C. for 3 hours in 15 ml of ethanol 30%, the mixture was dried at 3 ° C. at 15-25 ° C. and 50-60% relative humidity. It was fermented for months and prepared by mixing filtration concentrated and hot air dried or spray dried extracts.

In the case of Example 6, 2 g of vinegar, 1 g of ginseng leaf, 1 g of green tea were mixed with 60 ml of ethanol 30%, extracted at 86 ° C. for 3 hours, filtered, concentrated, hot air dried or spray dried to prepare an extract.

In the case of Example 7, 2 g of Eoseongcho, 1 g of Japonica and 1 g of green tea were mixed in 60 ml of ethanol 30%, and extracted at 86 ° C. for 3 hours, followed by temperature conditions of 15 to 25 ° C. and relative humidity of 50 to 60%. Fermented for 3 months in, filtered concentrated and hot air dried Or spray dried extract was prepared by mixing.

In Example 8, after extracting 1 g of eosungcho, 1 g of ginseng leaf, and 1 g of green tea at 86 ° C. for 3 hours in 15 ml of ethanol 30%, 3 at 15-25 ° C. and 50-60% relative humidity conditions It was fermented for months and prepared by mixing filtration concentrated and hot air dried or spray dried extracts.

In the case of Example 9, 2 g of Echoseongcho, 1 g of Japonica, and 1 g of green tea were mixed in 60 ml of ethanol 30%, extracted at 86 ° C. for 3 hours, filtered, concentrated and hot air dried or spray dried to prepare an extract.

In the case of Example 10, 2 g of Echochocho, 1 g of Japonica, and 1 g of green tea were mixed in 60 ml of ethanol 30%, and extracted at 86 ° C. for 3 hours, followed by temperature conditions of 15 to 25 ° C. and relative humidity of 50 to 60%. It was fermented for 3 months in, prepared by mixing filtration concentrated and hot air dried or spray dried extract.

2. BrdU  assay

(1) experimental principle

Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for staining nascent (early) DNA in living cells or tissues. BrdU is used instead of thymidine in the replicating DNA in the "S" phase, when DNA in the nucleus is doubled in the cell cycle, and the amount of synthesized DNA is confirmed by staining with BrdU antibody. Can be.

(2) Experiment method

1) Culture medium 1ml into the tube containing the cell pellet was suspended.

2) 10μl into the Haemacytometer (hemocytometer) and observed the number of cells under a microscope.

3) 1 ㅧ 10 ㅧ cells were seeded into 96 wells for the experiment. At this time, 100 μl of media was added per well. After seeding, wells were stored in a 37 ° C. CO ₂ incubator.

4) After storage for 24 hours in a 37 ℃ CO ₂ incubator, it was treated according to the compositions of Examples and Comparative Examples, and stored in 37 ℃ CO ₂ incubator.

5) After 24 hours storage at 37 ℃ CO ₂ incubator 10μl 10X BrdU solution was added and incubation for 1 hour and then the medium was removed.

6) Add 100µl of Fixing / Denaturing solution, leave at room temperature for 30 minutes, remove the solution, add 100µl of 1X Dectection antibody solution, and leave at room temperature for 1 hour.

7) washed three times with Washing buffer.

8) Treated with 1X HRP-conjugated secondary antibody solution at 100 μl per well and left for 30 minutes at room temperature.

9) washed three times with Washing buffer.

10) 100 μl of TMB Substrate was added to each well, stored at room temperature for 30 minutes, and then 100 μl of STOP solution was added to each well.

11) The absorbance of each well was measured at a wave length of 450 nm using an ELISA reader.

(3) experimental results

Table 3 shows the absorbance value according to the treatment concentration of the embodiment, the higher the absorbance means that the better the hair loss prevention, improvement or treatment effect.

3. MTT  assay

(1) experimental principle

As a technique to accurately measure the proliferation of cells or living cells, the MTT assay is a colorimetric assay that measures the absorption of a specific wavelength through color change. By dehydrogenase action, mitochondria reduce yellow soluble MTT tetrazolium to purple non-soluble MTT formazan (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide). A test that uses abilities. The higher the absorbance, the greater the number of living cells, and the lower the number of dead cells.

(2) Experiment method

1) Using a 1ml pipette, 1 ml of culture medium was placed in a 15-ml tube with cell pellet and tappin.

2) 10μl into a Haemacytometer (hemocytometer) and observed the number of cells under a microscope.

3) 1 ㅧ 10⁴ cells were seeded into 96well for MTT experiment. At this time, 100 μl of media was added per well.

4) Cells were seeded and stored in a 37 ° C. CO ₂ incubator.

5) After 24 hours storage in a CO ₂ incubator and treated according to the compositions of Examples and Comparative Examples, it was stored in 37 ℃ CO ₂ incubator.

6) After 24 hours of storage in a CO ₂ incubator, media was removed from the well using a media suction.

7) Put 100µl of DPBS per well and wash.

8) Prepare a solution of 10 μl of MTT solution in 90 μl of culture medium, and add 100 μl to each well.

9) Stored in a 37 ° C. CO ₂ incubator for 30 minutes up to 4 hours. (Each experiment used the same incubator.)

10) Absorbance was measured at a wavelength of 450 nm using an ELISA reader.

(3) experimental results

Table 4 shows the absorbance value according to the treatment concentration of the embodiment, the higher the absorbance means that the better the hair loss prevention, improvement or treatment effect.

In vivo  Experiment

1. Purpose of animal experiment

The causes of hair loss include heredity, stress, and aging, but since Hamilton (1942) discovered a relationship with testosterone, it is known that testosterone secretion is the cause. Dihydrotestosterone (DHT) binds to androgen receptors in hair follicles, delaying protein synthesis and shortening hair follicle growth phases, and the hair follicles quickly end and enter the resting phase, which increases the proportion of resting hair follicles. It occurs because it is thin and short. 5-α-reductase in hair follicle cells is an enzyme that converts testosterone, a testosterone, into DHT, a more powerful metabolite, and has about 10 times stronger hormone activity than normal testosterone and acts on the hair roots to inhibit hair growth.

After discovering the relationship with testosterone, a lot of research has been done on testosterone, but the cause or mechanism of hair loss is not yet clear. Currently developed and commercialized products have problems such as side effects such as abnormalities in the scalp.

Thus, this experiment was conducted to identify the in vivo effects of the present invention, including natural medicines that can exhibit the effect of preventing, treating or improving hair loss while solving the above problems.

2. Experimental method

(1) animal experiment

Six-week-old male C57BL / 6 mice were purchased and administered freely to feed for experimental animals under rearing conditions in which the contrast was automatically controlled at a temperature of 24 ° C ± 0.5 ° C, 55 ± 5% humidity, and 12-hour cycles.

C57BL / 6 mice are 6-7 weeks old and most of the hair is at rest and the skin color is pink. After adapting to the environment for about 7 days, it was used for the experiment.

Under the anesthesia through intraperitoneal injection, the hair was removed by using a hair clipper to prevent damage to the skin, and the animals were randomly divided into polycarbonate kennels and divided into groups.

The defined natural product complexes in each group were administered orally for 4 weeks as doses.

In the positive comparative example, oral finasteride was administered at 0.5 mg / day (Hui-Wen Chiu et al. 2013). Oral administration was performed by a skilled investigator because of concerns about inflammation and damage in a way that is invasive to the esophagus (or airway). All animal experiments should be conducted in accordance with the guidelines of the Orthodontic Animal Experimentation Ethics Committee (2015-0198-01). Details of each group are as follows.

1) normal rat (n = 3)

2) vehicle-negative control (n = 3)

3) finasteride-positive control (n = 3)

4) Example 3 200 mg / kg (n = 4)

5) Example 3 400 mg / kg (n = 4)

(2) visual observation

In order to visually check the condition of the hair after the start of the experiment, take a picture using a digital camera. According to the start time, 1 week, 2 weeks, and 3 weeks after the photograph and the hair condition of the back region, the grades of 0 to 4 are designated and compared.

0: when 0% to 20% of hair is regenerated

1: 20% to 40% hair regeneration

2: 40% to 60% hair regeneration

3: 60% to 80% hair regeneration

4: 80% to 100% hair regenerated

(3) histological examination

Four weeks after the start of the experiment, all animals were sacrificed and the skin was removed and immediately fixed in 10% formalin. The immobilized tissues were dehydrated with alcohol and xylene, embedded in paraffin, and 4 μm thick sections were prepared and stained with hematoxylin-eosin to observe histological changes under a microscope.

(4) Expression of IGF- 1 , known as hair growth factor, and TGF - β1 , a hair growth inhibitory and hair loss-inducing factor

In 50 mg of skin tissue such as Insulin-like growth factor (IGF-1) and TGF-β1 contained in the tissue, stored at -80 ° C, lysis buffer (50 mM Tris-HCl, 120 mM NaCl, 2 nM EDTA, 1 mM EGTA, 1% Triton X-100) 200ul was added, pulverized using a Homogenizer (Tissue tearor, Biospec, Korea), centrifuged, and the protein was quantified using Lowly method. 50 ug of protein was electrophoresed on a 15% SDS-PAGE gel, transferred to PVDF paper, blocked with 5% skim milk for 1 hour, and the primary antibody (IGF-1, TGF-β1, abcam, Cambridge, UK) The solution was diluted 1: 1000 in 5% skim milk and overnight at 4 ° C. After washing with 1X PBST, secondary antibody (Goat anti-Rabbit IgG) was diluted 1: 1000 in 5% skim milk and reacted for 1 hour, followed by washing, using ECL solution (Amersham, Arlington height, IL, USA). Analyze with an image analyzer (Image Analiyzer system, Syngene, UK).

3. Results

(1) visual observation

Prosca and test substance were orally administered 1 day after hair removal. Almost no hair grew until 5 days after administration in all experimental groups. The positive and test substance groups began to grow more hair than the negative comparisons after 15 days, and after 25 days, the hairs grew black and black in the groups except negative comparison groups. Table 5 shows the grades of each group which were observed by the naked eye.

 (2) Hematoxylin  and Eosin (H & E)

In order to investigate the effect of the test substance on the hair growth, the oral administration of the substance was followed by autopsy to observe the hair growth effect histologically.

In negative control, hair remained at rest, while positive control and test substance groups (Example 3 200mg / kg and 400mg / kg) increased the number and size of hair follicles significantly. It was. Normal group, growth phase, negative comparisons are at rest, positive comparisons induce hair follicle growth in growth phase, Example 3 200mg / kg group is shown to promote hair follicle growth in growth phase, Example 3 The 400 mg / kg group has also been shown to shorten the time for rapid hair follicle growth and hair regrowth in the growing phase.

Test substances vary in dosage and appear to promote, promote hair growth and help hair growth.

(3) IGF -One, TGF - β1  Western blot analysis

The expression of IGF-1, hair growth inhibitory, and TGF-β1, a hair loss-inducing factor, was higher in the positive comparison than in the negative comparison. Example 3 400 mg / kg Example 3 It was confirmed that the hair growth factor is overexpressed than the 200mg / kg group.

TGF-β1, a hair loss-inducing factor, was shown to be overexpressed in the negative comparison compared to the positive comparison, and Example 3 400mg / kg group was found to be lower TGF-β1 than Example 3 200mg / kg group.

4. Conclusion

In this experiment, the oral administration of natural complex using Echochocho, Japonica and Green Tea confirmed the gross and histological effects of hair growth. C57BL / 6 mice are a hair growth model and are used for the study of alopecia because their hair is black and histologically and immunologically very similar to human conditions. The experiment was started after confirming the resting period after depilation using male C57BL / 6 7 weeks old. After four weeks of oral administration of distilled water, positive control prosca, Example 3 200 mg / kg or Example 3 400 mg / kg, the positive comparison and the test substance group (Example 3 200 mg / kg and 400 mg / kg) began to grow more hair after 15 days compared to the negative comparison. After the 25th day, the hair grew black and filled the skin in the other groups except the negative comparison group. As a result of the H & E experiment, the negative comparison maintained the resting period, the positive comparison induced hair follicle growth in the growth phase, and Example 3 200mg / kg group improved the hair follicle growth in the growth phase, Example 3 400mg / kg. In the archipelago, the hair follicle growth and hair regrowth were shortened.

The expression of IGF-1 and TGF-β1 was measured 4 weeks after oral administration of test substance, and the expression level of IGF-1 was increased in 400 mg / kg of test substance Example 3 compared to the normal group, and IGF-1 in the negative comparison example. The expression was low and the expression of TGF-β1 was high.

TGF-β1, which is frequently expressed in Catagen and Teolgen periods, was the most expressed in the negative comparison cases, and in the test substance group, the expression of TGF-β1 was lowered, and the expression of IGF-1, a hair growth factor, was increased. It was confirmed that the effect of promoting hair growth by inducing factors that switch from the resting phase to the growth phase.

Example 3 200mg / kg and Example 3 400mg / kg as a test substance has a significant difference in the effect according to the dose, Example 3 in the group treated with 400mg / kg hair follicle than 200mg / kg treated group The number of cells increased and the depth of hair follicles also increased. According to the above results, the composition according to the present invention may promote and promote hair follicle growth and help hair growth.

Claims (12)

Eochocho extract, Fructus leaf extract and Green tea extract, Pharmaceutical composition for the prevention or treatment of hair loss comprising 25 to 75 parts by weight of Japonica leaf extract and 25 to 75 parts by weight of green tea extract based on 100 parts by weight of the Echochocho extract. The pharmaceutical composition for preventing or treating hair loss as set forth in claim 1, wherein the Echochocho extract, Japonica extract, and Green Tea extract are each independently extracted and mixed. The pharmaceutical composition for preventing or treating hair loss according to claim 1, wherein the extract of Echochocho, extract of Japonica leaves and Green Tea extract are not fermented. The pharmaceutical composition for preventing or treating hair loss according to claim 1, further comprising one or more extracts selected from the group consisting of black bean extract, glutinous rice extract, kelp extract, cheongung extract, nasturtium extract, root root extract and licorice extract. Eochocho extract, Fructus leaf extract and Green tea extract, Health functional food for the prevention or improvement of hair loss comprising 25 to 75 parts by weight of the extract of Japonica leaf and 25 to 75 parts by weight of green tea extract with respect to 100 parts by weight of Eochocho extract. The health functional food for preventing or improving hair loss of claim 5, wherein the Echo-chocho extract, Ja-yo-leaf extract and Green tea extract are each independently extracted and mixed. The dietary supplement for preventing or improving hair loss according to claim 5, wherein the extract of Echochocho, extract of Japonica and Green Tea extract are not fermented. The health functional food for preventing or improving hair loss of claim 5, further comprising one or more extracts selected from the group consisting of black bean extract, glutinous rice extract, kelp extract, Cheongung extract, nasturtium extract, root root extract and licorice extract. Eochocho extract, Fructus leaf extract and Green tea extract, Cosmetic composition for the prevention or improvement of hair loss comprising 25 to 75 parts by weight of the extract of Japonica leaf and 25 to 75 parts by weight of green tea extract with respect to 100 parts by weight of the Echochocho extract. The cosmetic composition for preventing or improving hair loss of claim 9, wherein the Echochocho extract, Japonica leaf extract and Green Tea extract are each independently extracted and mixed. The cosmetic composition for preventing or improving hair loss according to claim 9, wherein the Echochocho extract, Japonica extract and Green Tea extract are not fermented. The cosmetic composition for preventing or improving hair loss according to claim 9, further comprising at least one extract selected from the group consisting of black soybean extract, glutinous rice extract, kelp extract, cheongung extract, nasturtium extract, gallium extract and licorice extract.

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