WO2014185610A1 - Composition for preventing hair loss or promoting hair growth by containing bran extract as active ingredient - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or promoting hair growth by containing a bran extract as an active ingredient. The pharmaceutical composition containing the bran extract as an active ingredient, according to the present invention, has a recognized efficacy for suppressing and preventing hair loss and promoting hair growth, and can be variously used in pharmaceutical, cosmetic, food, and beauty care fields or the like.

Description

Hair loss prevention or hair growth promoting composition comprising bran extract as an active ingredient

The present invention relates to a composition for preventing hair loss or promoting hair growth comprising bran extract as an active ingredient.

The human hair has about 100,000 to 150,000 pieces and is formed in "hair follicles". There is a papilla in the hair follicle, where the small blood vessels are distributed, providing the nutrients needed for hair growth and a seborrheic gland on the side of the teat that provides the oil to lubricate the hair.

Each hair has a different cycle and grows and falls through the anagen, catagen and telogen phases. This cycle is repeated over three to six years, with an average of 50 to 100 hairs falling out daily.

In general, alopecia refers to an abnormally large number of hairs falling out due to shortening of the growth phase hair and more degenerative or resting hairs during these cycles.

The causes of hair loss are discussed, such as hyperthermia of male hormone action, excessive sebum secretion, poor blood circulation, hypoxia caused by peroxide, bacteria, genetic factors, aging, stress.

Specifically, testosteron, a type of male hormone, is activated as dihydrotestoserone (DHT) by an enzyme called 5α-reductase. Hair loss occurs by this DHT binding to specific receptors and inducing proteins that cause hair loss. In addition, this mechanism may produce excessive sebum, causing acne or seborrheic dermatitis, and causing hair loss with inflammation on the scalp.

Drugs known to prevent hair loss and promote hair growth and hair growth include minoxidil, trichosaccharide, and finasteride. Minoxidil was initially developed as a vasodilator for the treatment of hypertension, but it was developed as a hair restorer as hirsutism was reported as a side effect. The mechanism of action of minoxidil on the hair growth effect has not been clarified to date, but the effect of increased nutrient supply through vasodilation, potassium channel opening effect, and inhibition of apoptosis of dermal papilla cells The back is thought to induce hair growth. Finasteride, developed by Merck, also inhibits the activity of 5α-reductase, an enzyme that acts on male hormone metabolism. It was initially developed for the treatment of enlarged prostates, but was later developed as a hair restorer, as it was known to promote hair growth. However, in the case of such preparations, there is an urgent need for the development of a composition that ensures safety and efficacy because of side effects such as lack of obvious efficacy and human stability and skin irritation.

Korean Patent Publication No. 2010-0111640 mentions hair loss prevention and hair growth promoting effects of one or more compositions selected from the group consisting of brown rice, black rice, and rice. However, no prior literature mentions bran hair loss prevention and hair growth promoting effect.

The present inventors have found that the bran extract plays an important role in the growth of hair and continued the study to complete the present invention.

It is an object of the present invention to provide a pharmaceutical composition comprising as an active ingredient bran extract that is safe for skin, prevents hair loss, and promotes hair growth.

Another object of the present invention is to provide a cosmetic composition comprising the bran extract as an active ingredient.

Still another object of the present invention is to provide a health functional food comprising the bran extract as an active ingredient.

Another object of the present invention to provide a hair loss prevention and hair growth promoting method comprising the step of treating a pharmaceutical composition comprising a bran extract at the hair loss site.

Still another object of the present invention is to provide a use of bran extract for use in the manufacture of a pharmaceutical composition for preventing hair loss and promoting hair growth.

The present invention provides a pharmaceutical composition for preventing hair loss or promoting hair growth comprising an extract of bran as an active ingredient.

The bran extract may be included in an amount of 0.01 to 50% by weight based on the total weight of the composition, but is not necessarily limited thereto. If outside the above range may not exhibit hair loss prevention or hair growth promoting effect.

The pharmaceutical composition may be selected from the group consisting of tablets, capsules, injections, creams, gels, patches, sprays, ointments, warnings, lotions, linen, pasta and cataplasma.

In another aspect, the present invention provides a cosmetic composition for preventing hair loss or promoting hair growth comprising an extract of bran as an active ingredient.

The bran extract may be included in an amount of 0.01 to 50% by weight based on the total weight of the composition, but is not necessarily limited thereto. If outside the above range may not exhibit hair loss prevention or hair growth promoting effect.

The cosmetic composition is a hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, hair massage cream, hair wax, hair aerosol , Hair pack, nourishment pack, hair soap, hair cleansing foam, hair oil, hair drier, hair preservative, hair dye, hair wave, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, It may be a formulation selected from the group consisting of hair mousse and hair spray.

In another aspect, the present invention provides a health functional food for preventing hair loss or promoting hair growth comprising an extract of bran as an active ingredient.

"Health functional food" as defined in the present invention means a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to Act No. 6767 of the Health Functional Food Act, and "functional" means a human body It means the ingestion for the purpose of obtaining a useful effect in health use such as nutrient control or physiological action on the structure and function of.

The bran extract may be included in an amount of 0.01 to 50% by weight based on the total weight of the composition, but is not necessarily limited thereto. If outside the above range may not exhibit hair loss prevention or hair growth promoting effect.

The health functional food may be a powder, granule, tablet, capsule or beverage.

In another aspect, the present invention provides a method for preventing hair loss and promoting hair growth, comprising treating a pharmaceutical composition including a bran extract to a hair loss site.

In another aspect the present invention provides the use of bran extract for use in the manufacture of a pharmaceutical composition for preventing hair loss and promoting hair growth.

The composition comprising the bran extract according to the present invention as an active ingredient provides an effect of inhibiting and preventing hair loss and promoting hair growth. The composition containing the bran extract of the present invention can be used in various fields such as pharmacy, cosmetics, food and beauty.

Figure 1 shows the dermal papilla cell proliferation efficacy of the bran hexane fraction.

Figure 2 is a diagram confirming the expression of cell cycle proteins and Wnt / beta-catenin signaling proteins in dermal papilla cells by the bran hexane fraction.

Figure 3 shows the hair-fiber growth effect of the bran hexane fraction in Vibrissa follicles.

4 is a diagram confirming in vitro the hair growth effect by the bran hexane fraction:

A: Time-dependent hair growth effect in the control group, bran hexane fraction treated group and minoxidil treated group (MINOXYL ^™ ) in rats; And

B: Hairy skin area (black part)% of whole skin.

Hereinafter, the present invention will be described in detail.

In the present invention, "wheat (Triticum aestivum L.)" is a perennial plant belonging to the monocotyledonous rice plant family. Wheat is known to have excellent anticancer and antioxidant effects.

“Bran” is the remainder of the separation of flour and embryos from flour, and contains most of the seed's blood but a small amount of embryos and embryos.

“Extract” as defined herein is a solvent selected from water, including purified water, lower alcohols having 1 to 4 carbon atoms or mixed solvents thereof, hexane, butylene glycol, propylene glycol, glycerin, ethyl acetate, ether, chloroform, preferably Includes extracts soluble in water or methanol mixed solvents.

The term "bran extract" includes all of them without particular limitation, as long as it is obtained by extracting an active ingredient from bran. For example, the result obtained by putting bran into water or an organic solvent and eluting an effective component through means, such as standing, stirring, pressurization, or heating, is mentioned. It also includes a freeze-dried product obtained by lyophilizing the liquid extract obtained as described above.

It also includes a powder obtained by grinding such lyophilisate. In addition, any means available to those skilled in the art for extracting the active ingredient includes all the extracted extracts, including those that have been processed after extraction and freeze drying. Others include extracts obtained by conventional extraction methods such as extraction by baths or room temperature, or by conventional extraction methods described in Chinese medicine or textbooks.

In addition, it includes a fraction extract obtained through the Stass Otto extraction method, various column chromatography methods, etc., which is a special extraction method for separating specific active compounds.

The bran extract may be used without limitation, such as being collected, commercially available, and may include, for example, using it after removing the foreign matter by washing with water to remove the foreign matter.

The bran extract may be obtained by conventional extraction methods in the art, such as ultrasonic extraction, filtration and reflux extraction.

Preferably, the bran may be an extract extracted with water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, a solvent selected from hexane, butylene glycol, propylene glycol, glycerin, ethyl acetate, ether, chloroform.

"Hair loss" refers to the phenomenon of hair dropping from the scalp or the condition of the hair being thin or thin. "Hair loss prevention" means preventing and suppressing hair loss as described above. "Raising hair growth" means not only promoting the production of new hair, but also ensuring that existing hair grows healthy.

The hair cycle can be divided into three main stages, known as the growing, degenerative and resting phases. In the growth phase, hair growth occurs as hair follicles grow deep into the skin with rapid proliferation of cells. The next phenomenon is the degenerative phase, which is a transitional period in which the disruption of cell division is noticeable, during which hair follicles gradually degenerate and hair growth stops. In the next phase, the resting phase, the degenerative hair follicles contain germs with densely packed dermal papilla cells. The onset of new growth phase phenomena at rest is induced by rapid cell proliferation in the embryo, swelling of the dermal papilla and the synthesis of basement membrane elements.

In general, "hair growth" occurs in the growth phase, and is promoted by induction from the resting phase to the growth phase and the delay from the growth phase to the regression phase.

Therefore, it is necessary to prevent hair loss, that is, to prevent hair loss or to cause hair regrowth, that is, to promote hair growth, by promoting or extending the growth phase.

The composition according to an embodiment of the present invention includes one or more of the effects of preventing existing hair from falling out, improving the thickened hair, and the like, and generating new hair. The composition according to the present invention can also induce hair growth by activating dermal papilla cells.

In a specific embodiment of the present invention, using the culture of hair papilla cells derived from hair follicles and rat vibrissa follicles, the bran hexane fraction was tested for hair growth efficacy.

In one embodiment of the present invention it was investigated using rat vibrissa immortalized dermal papilla cells for the effect of the dermal papilla cells proliferation. When the bran hexane fractions were treated at concentrations of 1, 10, 50 and 100 μg / ml, the growth and proliferation of the dermal papilla cells was 115.1 ± 5.2% (P <0.01), 139.3 ± 5.4% (P <0.001), 190.5 ± 6.5 % (P <0.001) and 183.3 ± 10.2% (P <0.0001) were increased. The growth proliferation effect of these dermal papilla cells is higher than the growth proliferation effect of the minoxidil dermal papilla cells used as a positive control material.

In one embodiment of the present invention it was confirmed the expression of proteins involved in the signal transduction pathway that plays an important role in hair growth, cell proliferation regulation in dermal papilla cells. Mammalian cells are known to require activation of the cyclin E / CDK2 complex, increased cyclin D1, and increased phosphorylation of pRB for cell cycle progression, a major process of cell proliferation (Sherr, CJ (1996). Science 274: 1672. . -1677, Sherr, CJ and Roberts , JM (1999) GenesDev 13:.... 1501-1512 Prall, OW, Sarcevic, B., Musgrove, EA, Watts, CK and Sutherland, RL (1997) J.Biol Chem. 272: 10882-10894.). Minoxidil is known to promote cell cycle progression and induce proliferation of dermal papilla cells by increasing cyclin E and CDK2 levels and decreasing p27 ^kip1 levels in dermal papilla cells (Kang, JI, Kim, EJ, Kim, MK, Jeon, YJ, Kang, SM, Koh, YS, Yoo, ES and Kang, HK (2013). Mar. Drugs 11 : 1783-1799.). In addition, the Wnt / β-catenin signaling pathway plays an important role in hair growth and cell growth regulation (Kwack, MH, Kang, BM, Kim, MK, Kim, JC and Sung, YK (2011). J. Dermatol. Sci 62:.. 154-159, Wangefjord , S., Braedstedt, J., Ericson Lindquist, K., Nodin, B., Jirstroem, K. and Eberhard, J. (2013) Diagn.Pathol 8:.. 10 .). The Wnt / β-catenin signaling pathway is regulated by a variety of factors, in particular the activation of Akt induces β-catenin phosphorylation (ser552) and GSK3β phosphorylation (ser9), while PKA is β-catenin phosphorylation (ser552 and ser675), which in turn activates the Wnt / β-catenin signaling pathway. Subsequently, processes such as stabilizing β-catenin and promoting migration to the cell nucleus occur to regulate expression of the target gene. Lee et al. Reported that minoxidil did not affect β-catenin levels in human DPCs and C3H mice, whereas Kwack et al. Found that minoxidil regulates β-catenin signaling pathways via activation of PKA, Akt and GSK3β in human DPCs. It has been reported to exhibit hair growth efficacy.

In addition, in one embodiment of the present invention while culturing the rat vibrissa follicles of the growth phase rats for 3 weeks, the hair growth efficacy of the bran hexane fraction treatment group was investigated. In particular, at the concentrations of 10 and 50 μg / ml, the bran hexane fraction treatment group showed higher growth efficiency of hair-fiber than the positive control 10 μM minoxidil.

Through the above embodiment, the bran extract can be seen that the hair loss prevention and hair growth promoting effect is excellent.

In conclusion, the bran extract of the present invention is expected to act to promote the growth of hair papilla cells, which play a very important role in hair growth, to induce the growth phase of hair follicles or to maintain the growth phase.

The pharmaceutical composition containing the bran extract of the present invention as an active ingredient is a tablet for preventing hair loss and promoting hair growth, a capsule, an injection, a cream, a gel, a patch, a spray, an ointment, a warning, a lotion, a linen, a pasta or a cata It may be prepared by using a pharmaceutical composition in the form of plasma, but is not limited thereto.

Preferred dosages of the bran extract of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route and duration of administration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the bran extract of the present invention can be used in various hair cosmetics having the effect of preventing hair loss and activating hair growth. Examples of products to which the composition can be added include, for example, hair tonics, hair conditioners, hair essences, hair lotions, hair nutrition lotions, hair shampoos, hair rinses, hair treatments, hair creams, hair nutrition creams, hair moisturizers. Cream, hair massage cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservative, hair colorant, hair wave agent, hair bleach, hair gel, hair glaze, Cosmetics such as hair dressers, hair lacquers, hair moisturizers, hair mousses or hair sprays and the like.

The cosmetic of the present invention may include a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins usable in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

Any useful vitamin can be used as long as it can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) and the like. Derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl) Ethyl ether) and the like are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound that can be formulated into cosmetics, but preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle. The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxy ethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeast or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Colorginine, arginic acid, sodium arginate, purified from these seaweed extracts, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristate, myristin acid myristyl, myristin acid cetyl, stearyl stearate, decyl oleate, lysinoole phosphate Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid stestearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctane neopentyl glycol, tricaprylate glyceryl, trifundecyl glyceryl, triisopalmitate, glyceryl triisostearate, octyl neopentane Dodecyl, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearic acid , Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauric lactate, myritic lactate, cetyl lactate, octyl decyl lactate, citric acid Triethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacic acid Dioctyl sebacate, stearic acid cholesterol, isostearic acid cholesterol, hydroxystearate cholesterol, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid phyteryl, phosphate oleate , 12-stearoylhydroxystearate isocetyl, 12-stealoylhydroxystearate stearyl, 12-ste Roil-hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, alpha-olefin oligomers, isoparaffins, ceresin, paraffins, liquid isoparaffins, polybutenes, microcrystalline waxes, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer. Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters. As the water-soluble polymer, carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, dextrin, etc. Can be mentioned.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE / POP (Polyoxyethylene / Polyoxypropylene) Copolymer, POE / POP Alkyl Ether, Polyether Modified Silicone, Laurin Acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl or phthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphorus salts, Alkylamide phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. Can be mentioned.

Examples of cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyl trimethylammonium chloride, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzyl ammonium bromide, and behenyltrimethylammonium bromide. And benzalkonium chloride, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengal, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments.

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as zinc sodium cetyl acid, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine Acyl basic amino acids; N-acylpolypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethylhexaneglyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Cananoic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hydric When the like can be mentioned 5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitrohydrol, undecylenic acid, and the like.

Examples of the antioxidants include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-50% weight percentage with respect to a total weight. It can be formulated as.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional stabilizers, solubilizers, vitamins, pigments and flavorings. And auxiliaries and carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

When the formulation of the present invention is a paste, cream or gel, animal fibers, plant fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

Solvents, solvating or emulsifying agents may be used as carrier components when the formulations of the invention are solutions or emulsions, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan, and the like.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspension agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the present invention also provides a health functional food for preventing hair loss or promoting hair growth comprising an extract of bran as an active ingredient.

Foods to which the bran extract of the present invention may be added include, for example, various foods, beverages, gums, teas, vitamin complexes, health supplements, and the like, and may be used in the form of powders, granules, tablets, capsules, or beverages. Can be.

Since the bran extract itself of the present invention has almost no toxicity and side effects, it is a medicament that can be used safely even when taken for long periods of time.

The bran extract of the present invention may be added to food or beverage for the purpose of preventing and improving hair loss disease. At this time, the amount of the extract in the food or beverage is generally added to the health food composition of the present invention 0.01 to 50% by weight of the total food weight, the health beverage composition is 0.02 to 10 g based on 100 ml, preferably It can be added at a ratio of 0.3 to 1 g.

In addition to containing the extract as an essential ingredient in the indicated proportions, the health beverage composition of the present invention has no particular limitation on the liquid component, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates are conventional monosaccharides such as disaccharides such as glucose and fructose, such as maltose, sucrose and the like, and polysaccharides such as dextrin, cyclodextrin and the like. Sugars and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of such natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, examples will be described in detail to help understand the present invention. However, the following examples are merely to illustrate the content of the present invention is not limited to the scope of the present invention. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example 1 Obtaining and Extracting Bran

The wheat bran used in the present invention is supplied from a mill of wheat bran grown in Gyeongsangbuk-do. 5 kg of total bran samples were extracted with water bath in a water bath three times for 4 hours in 90% ethanol. Then, the supernatant was collected, concentrated under reduced pressure using a rotary evaporator, and 337 g of crude extract was obtained. 300 g of the solvent was fractionated with n-hexane to obtain 133 g of an n-hexane layer, and this fraction was used as a sample for the experiment.

Example 2 Proliferation Efficacy of Breast Papillary Cells

<Example 2-1> Confirmation of proliferation of dermal papilla cells

Rat vibrissa immortalized dermal papilla cells isolated from rat whiskers were treated with 100 units / ml penicillin-100 μg / ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated fetal bovine serum. DMEM (Hyclone Inc, USA) medium containing inactivated fetal bovine serum, FBS; Gibco Inc, NY, USA) was incubated at 37 ° C., 5% CO ₂ incubator, and passaged once every 3 days.

Proliferation of dermal papilla cells was measured using an MTT assay. 1.0 × 10 ⁴ cells / ml of dermal papilla cells were suspended in DMEM medium containing 1% FBS, placed in a 96 well plate and incubated for 24 hours, and then the bran hexane fractions of 0.1, 1, 10, 50 and Treatment was at a concentration of 100 μg / ml. The positive control minoxidil (minoxidil, Sigma, MO, USA) was treated at a concentration of 10 μM.

After incubation for 4 days, 50 μl of MTT (Sigma, MO, USA) was added and reacted for 4 hours. The supernatant was removed, 200 μl of DMSO was added to dissolve the precipitate, and the absorbance was measured at 540 nm using a VERSAmax microplate reader (Molecular Devices, Calif., USA). The average absorbance value for each sample addition group was obtained, and the degree of proliferation was investigated by comparing with the absorbance values of the control group.

All measurements were expressed as mean ± standard deviation, and statistical significance test was conducted by Student's t-test, and the significance was recognized when p-value was 0.05 or less. Statistical processing was performed using SPSS 12.0K (Release 12.0.1. SPSS Inc. USA) for Windows.

As a result, when the bran hexane fraction was treated at concentrations of 0.1, 1, 10, 50 and 100 μg / ml, it was 106.9 ± 11.5%, 115.1 ± 5.2% (P <0.01) and 139.3 ± 5.4% (P <0.001), 190.5 ± 6.5% (P <0.001) and 183.3 ± 10.2% (P <0.001) increased the proliferation of dermal papilla cells (Table 1, FIG. 1).

Table 1 sample Concentration (/ ml) Dermal papilla cell growth rate (%) Minoxidil 10 M 112.3 ± 2.7 ( P <0.01 ) Bran Hexane Fraction 0.1 106.9 ± 11.5 ( P <0.01 ) One 115.1 ± 5.2 ( P <0.01 ) 10 139.3 ± 5.4 ( P <0.001 ) 50 190.5 ± 6.5 ( P <0.001 ) 100 183.3 ± 10.2 ( P <0.001 )

In particular, the bran hexane fractionation treatment group at the concentration of 10, 50 and 100 μg / ml showed a proliferative effect higher than 112.3 ± 2.7% (P <0.01) of the growth effect of 10 μM minoxidil used as a positive control, the bran hexane fraction It shows that it can exhibit hair growth efficacy like minoxidil.

<Example 2-2> Confirmation of cell cycle protein expression in dermal papilla cells

In order to elucidate the molecular mechanism by which bran extract (hexane) extract increases the growth of dermal papilla cells, cell cycle protein expression was observed. Specifically, bran extract (1, 10 and 50 μg / ml) after incubating Dermal papilla cells (1.0 × 10 ⁵ cells / ml in 100 mm dish) in DMEM medium containing 1% FBS for 24 hours. ) And 10 μM minoxidil, respectively, and incubated for 24 hours. Cells were washed twice with PBS and then 200 μl of lysis buffer [50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM EDTA, 1 mM EGTA, 1 mM NaVO3, 10 mM NaF, 1 mM dithiothreitol (DTT). ), 1 mM phenylmethylsulfonylfluoride (PMSF), 25 μg / mL aprotinin, 25 μg / mL leupeptin and 1% NP-40] were added and then cell disrupted at 4 ° C. for 30 minutes. Cell lysate was centrifuged at 15,000 rpm for 15 minutes to obtain supernatant and stored at −20 ° C. until used in the experiment. Protein concentration was quantified by the Bradford method using bovine serum albumin (BSA) as a standard. 20-30 μg of the cell lysate was denatured with 8-12% mini gel SDS-PAGE (Sodium dodecyl sulfate polyacrylamide gel electrophoresis) and at 200 mA with a polyvinylidene fluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) Transferred for 2 hours. In addition, blocking of the membrane was carried out for 2 hours in a solution of Tween-20-TBS (T-TBS) (50 mM Tris, pH 7.6, 150 mM NaCl, 0.1% Tween-20) containing 5% nonfat dried milk. Cyclin D1 (1: 1000), phospho-CDK2 (1: 1000), Cyclin E (1: 2000), phospho (ser780) -pRB (1: 1000), phospho-ERK1 / 2 (1) : 1000), ERK1 / 2 (1: 1000), phospho (ser552) -β-catenin (1: 1000), phospho (ser675) -β-catenin (1: 1000), β-catenin (1: 2000), Primary antibodies to phospho (ser9) -GSK3β (1: 1000), GSK3β (1: 1000) and β-actin (1: 5000) were added and then overnight at 4 ° C. The secondary antibody (Secondary anbibody) was subjected to an antibody binding reaction at room temperature for 1 hour using an antibody diluted 1: 1: 5000 anti-rabbit IgG, HRP (Horse Radish Peroxidase) conjugated anti-rabbit IgG, anti-mouse IgG. Thereafter, the membrane was washed three times with T-TBS, and then reacted with ECL substrate (Intron, Seoul, Korea) for 1 minute and then photosensitive to X-ray film (AGFA, Mortsel, Belgium).

As a result, when the bran hexane extract was treated with 1, 10 and 50 μg / ml concentrations in the dermal papilla cells for 24 hours, the expression of cyclin D1, cyclin E, phospho-CDK2 and phospho-pRB was increased. Treatment of minoxidil also showed similar results with bran hexane extract (FIG. 2A). In addition, it was confirmed that the activation of Phospho (ser552) -β-catenin, Phospho (ser675) -β-catenin, and Phospho (ser9) -GSK3β increased the treatment of bran hexane extract. Was somewhat activated (FIG. 2B). These results indicate that bran hexane extracts proliferate the dermal papilla cells by activating Wnt / β-catenin signaling pathways through the GSK3β, PKA and Akt pathways as well as cell cycle proteins such as cyclin D1, cyclin E, phospho-CDK2 and phospho-pRB. Suggests increased potency.

Example 3 Hair Growth Efficacy Measurement: Hair-fiber Length Growth Measurement

Example 3-1 Isolation and Culture of Rat Vibrissa Follicles

Three-week-old male Wistar rats (Japan SLC, Hamamatsu, Janpan) were purchased from a central laboratory animal and anesthetized with ethyl ether, and then cervical spine was killed. Rat left and right mystacial pads were isolated and E / P buffer [Earle's balanced salts] containing 100 units / ml penicillin-100 μg / ml streptomycin (Gibco Inc, NY, USA) solution (EBSS, Sigma MO, USA) + phosphate-buffered saline (PBS, Sigma MO, USA)]. Vibrissa follicles were carefully separated while observing under a dissecting microscope. Until the hair follicles were separated, the separated hair follicles were placed in a Petri dish containing E / P buffer and incubated at 37 ° C. in a 5% CO ₂ incubator for about 1 hour. 2 mM L-glutamine (Gibco Inc, NY, USA), 10 μg / ml insulin (Sigma MO, USA), 50 nM hydrocortisone (Sigma MO, USA) and 100 units / Add 500 μl of William E medium (Gillco E Inc., NY, USA) containing ml penicillin-100 μg / ml streptomycin and place one hair follicle in one medium at 37 ° C., 5% CO ₂ thermostat. Incubated at. During the culture the medium was changed every 3 days and the bran hexane fractions were treated at concentrations of 1, 10 and 50 μg / ml. The positive control minoxidil was treated at a concentration of 10 μM (Kang et al., 2012 Biomol Ther 20 (1), 118-124).

Example 3-2 Measurement of Vibrissa follicles Growth

Vibrissa follicle morphology in culture was photographed using a microscope (Olympus, Japan). Hair follicle length was measured using an image analysis program (image analyzer, DP controller; Olympus, Japan). The average value of the hair follicle length change was calculated and compared with the average length of the control group.

All measurements were expressed as mean ± standard error. Statistical significance test was performed by Student's t-test, and the significance was recognized when p-value was 0.05 or less. Statistical processing was performed using SPSS 12.0K (Release 12.0.1. SPSS Inc. USA) for Windows.

After comparing the length difference (%) of hair follicles between the bran hexane fraction treatment group and the control group on the 21st day, hair fiber growth was 150.0 ± 21.7% (P <) when 1, 10 and 50 μg / ml were treated, respectively. 0.001), 203.6 ± 7.6% (P <0.001) and 246.4 ± 19.8% (P <0.001) (Table 2, FIG. 3).

TABLE 2 sample Concentration (/ ml) hair fiber growth growth rate (%) Minoxidil 10 M 156.3 ± 11.5 ( P <0.05 ) Bran Hexane Fraction One 150.0 ± 21.7 ( P <0.001 ) 10 203.6 ± 7.6 ( P <0.001 ) 50 246.4 ± 19.8 ( P <0.001 )

In particular, the hair-fiber growth increase effect of the bran hexane fraction at concentrations of 10 and 50 μg / ml was greater than that of 156.3 ± 11.5% (P <0.05) growth of minoxidil (10 μM) as a positive control. Higher.

Example 4 Hair growth efficacy measurement: in vivo experiment

Example 4-1 Experimental Animal

Six-week-old female C57BL / 6 mice were purchased from Orient Bio Co., Ltd. and used for experiments after adapting to the animal breeding environment for one week. Animal breeding room was maintained day and night at a temperature of 23 ± 3 ℃, 12 hours. Experimental animals were reared in mouse cages for isolation and were fed freely with experimental feed. The experimental group was set up in five groups, which were treated with MINOXYL ^TM (modern drug) and bran hexane extracts (10, 50 and 100 μg / ml), which were the control group and the positive control group. Eight mice in each experimental group were used for the experiment.

Example 4-1 Measurement of Hair Growth Efficacy

To examine the hair growth effect, we used 7-week-old rest hairs with pink dorsal skin color. The back of the mouse was completely removed with a small animal clipper. Once a day, 200 μl of the sample was dropped onto the epilating area, and then applied by tapping and 50% ethanol was applied to the control group. On the 1st, 15th, 22nd, 29th and 35th day after the start of the experiment, photographs were taken to check the hair growth condition, and dotmatrix planimetry was performed for quantitative evaluation.

As a result, the change in skin color was observed from 22 days after hair removal in the bran hexane extract application group, and the change in skin color and hair growth was confirmed in the bran hexane extract application group at 29 and 35 days after hair removal. Quantitative analysis of the hair growth induction effect of the bran hexane extract application group using dotmatrix area measurement method, the bran hexane extract application group at the concentration of 50 μg / ml at day 35 showed significantly higher growth induction effect than the control group It was confirmed that it is shown (Fig. 4). In addition, the positive control group MINOXYL ^TM (containing 5% minoxidil) coating group was able to confirm the change of skin color and the apparent hair growth effect after 15 days after depilation (Fig. 4). These results suggest that bran hexane extract can induce hair growth phase in vivo .

Example 5 Statistical Analysis

All results were expressed as mean ± standard error. Statistical significance test was performed by student's t-test, and the significance was recognized when p-value was less than 0.05. For statistical processing, SPSS 12.0K for Windows (Release 12.0.1. SPSS Inc. USA) was used.

In the present invention, the bran hexane extract induces hair follicle growth phase or growth phase through the effect of promoting cell growth proliferation by activation of cell cycle proteins, Wnt / β-catenin signaling pathway in the dermal papilla cells that play an important role in hair growth. It is maintained. These findings provide evidence that bran hexane extract has the potential to be used as a treatment and prevention of hair loss.

Hereinafter, an example of the preparation of the composition according to an embodiment of the present invention will be described, but the present invention is not intended to be limited thereto but merely to be described in detail.

Formulation Example 1 Preparation of Hair Tonic

Formulation Example 2 Preparation of Hair Conditioner

Formulation Example 3 Preparation of Hair Lotion

Formulation Example 4 Preparation of Hair Soap

Formulation Example 5 Hydrophilic Ointment

Formulation Example 6 Preparation of Tablet

According to the conventional method of preparing tablets, the above ingredients were added to the indicated contents, mixed uniformly, stirred and granulated. After drying, using a tableting machine, a desired tablet containing 33.3 wt% of the bran extract, which is an active ingredient, was prepared.

Formulation Example 7 Preparation of Capsule

According to the conventional method for preparing the capsules, the ingredients are added to a given content, mixed uniformly, and then filled into gelatin capsules of an appropriate size so that 33 wt% of the bran extract, an active ingredient, is contained per capsule. Prepared.

Formulation Example 8 Preparation of Liquid

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution is prepared by sterilization.

Formulation Example 9 Preparation of Healthy Food

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food according to a conventional method.

Formulation Example 10 Preparation of Healthy Drinks

After mixing the above components according to a conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and refrigerated and then stored in the present invention For the manufacture of healthy beverage compositions.

Although the composition ratio is a composition that is relatively suitable for the preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

Claims (11)

Hair loss prevention or hair growth promoting pharmaceutical composition comprising an extract of bran as an active ingredient. The method of claim 1, The bran extract is contained in 0.01 to 50% by weight relative to the total weight of the composition. The method according to claim 1 or 2, The composition is a tablet, capsules, injections, creams, gels, patches, sprays, ointments, warnings, lotions, linings, pasta and cataplasma formulations selected from the group consisting of preventing hair loss or promoting hair growth Pharmaceutical composition for. Hair loss prevention or hair growth promoting cosmetic composition comprising an extract of bran as an active ingredient. The method of claim 4, wherein The bran extract is a cosmetic composition that comprises from 0.01 to 50% by weight relative to the total weight of the composition. The method according to claim 4 or 5, The composition includes hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nourishment cream, hair moisturizer cream, hair massage cream, hair wax, hair aerosol, Hair Pack, Hair Nutrition Pack, Hair Soap, Hair Cleansing Foam, Hair Oil, Hair Dryer, Hair Preservative, Hair Dye, Hair Wave, Hair Bleach, Hair Gel, Hair Glaze, Hair Dresser, Hair Lacquer, Hair Moisturizer, Hair A cosmetic composition for preventing hair loss or promoting hair growth, characterized in that the formulation is selected from the group consisting of mousse and hairspray. Health functional food for preventing hair loss and promoting hair growth comprising an extract of bran as an active ingredient. The method of claim 7, wherein The bran extract is a health functional food containing 0.01 to 50% by weight based on the total weight of the composition. The method according to claim 7 or 8, The health functional food is a powder, granules, tablets, capsules or beverages for hair loss prevention and hair growth promoting health functional food. Hair loss prevention and hair growth promoting method comprising the step of treating the pharmaceutical composition comprising a bran extract at the hair loss site. Use of bran extract for use in the manufacture of a pharmaceutical composition for preventing hair loss and promoting hair growth.

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