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WO2016184062A1 - Biosensor based on photoelectrically responsive semiconductor nanomaterial - Google Patents

Biosensor based on photoelectrically responsive semiconductor nanomaterial Download PDF

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Publication number
WO2016184062A1
WO2016184062A1 PCT/CN2015/095041 CN2015095041W WO2016184062A1 WO 2016184062 A1 WO2016184062 A1 WO 2016184062A1 CN 2015095041 W CN2015095041 W CN 2015095041W WO 2016184062 A1 WO2016184062 A1 WO 2016184062A1
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Prior art keywords
responsive semiconductor
cds
photoelectrically responsive
semiconductor nanomaterial
photoelectrically
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PCT/CN2015/095041
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French (fr)
Chinese (zh)
Inventor
周明
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NANTONG MINICHIP MICRO-ELECTRONICS Co Ltd
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NANTONG MINICHIP MICRO-ELECTRONICS Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Definitions

  • the invention relates to a biosensor based on photoelectric responsive semiconductor nano material, and belongs to the technical field of materials.
  • a biosensor is an instrument that is sensitive to biological substances and converts its concentration into electrical signals for detection. It is made up of immobilized bio-sensitive materials as identification elements (including enzymes, antibodies, antigens, microorganisms, cells, tissues, nucleic acids and other biologically active substances), appropriate physical and chemical transducers (such as oxygen electrodes, photosensitive tubes, FETs, An analytical tool or system consisting of a piezoelectric crystal or the like and a signal amplifying device. Biosensors have the function of a receiver and a converter.
  • the biosensor based on the photoelectric responsive semiconductor nanomaterial of the present invention comprises a molecular recognition portion and a conversion portion, wherein the molecular recognition portion is selected from a photoelectrically responsive semiconductor nano material, and the photoelectric responsive semiconductor nano material is prepared by the following method. :
  • the surfactant is polyethylene glycol.
  • the biosensor based on the photoelectric responsive semiconductor nano material of the invention has high sensitivity and good selectivity.
  • the biosensor based on the photoelectric responsive semiconductor nanomaterial of the present invention comprises a molecular recognition portion and a conversion portion, wherein the molecular recognition portion is selected from a photoelectrically responsive semiconductor nano material, and the photoelectric responsive semiconductor nano material is prepared by the following method. :
  • the surfactant is polyethylene glycol.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
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  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Silicon Compounds (AREA)
  • Light Receiving Elements (AREA)

Abstract

A biosensor based on a photoelectrically responsive semiconductor nanomaterial. The biosensor comprises a molecule identifying portion and a transducer portion. A photoelectrically responsive semiconductor nanomaterial is selected for the molecule identifying portion, and is prepared with the following method: 1) preparing a silica crystal from colloidal silica, and obtaining nanorods on a surface of a gold piece with E-beam evaporation; 2) preparing CdS particles with a hydrothermal process, and performing a surface modification treatment on the CdS nanoparticles with a surfactant; and 3) adsorbing the CdS nanoparticles onto the surfaces of the nanorods to obtain the SiO2-CdS photoelectrically responsive semiconductor nanomaterial. The biosensor based on the photoelectrically responsive semiconductor nanomaterial has high sensitivity and good selectivity.

Description

基于光电响应性半导体纳米材料的生物传感器Biosensor based on photoelectric responsive semiconductor nanomaterial 技术领域Technical field

本发明涉及一种基于光电响应性半导体纳米材料的生物传感器，属于材料技术领域。The invention relates to a biosensor based on photoelectric responsive semiconductor nano material, and belongs to the technical field of materials.

背景技术Background technique

生物传感器(biosensor)，是一种对生物物质敏感并将其浓度转换为电信号进行检测的仪器。是由固定化的生物敏感材料作识别元件(包括酶、抗体、抗原、微生物、细胞、组织、核酸等生物活性物质)、适当的理化换能器(如氧电极、光敏管、场效应管、压电晶体等等)及信号放大装置构成的分析工具或系统。生物传感器具有接受器与转换器的功能。A biosensor is an instrument that is sensitive to biological substances and converts its concentration into electrical signals for detection. It is made up of immobilized bio-sensitive materials as identification elements (including enzymes, antibodies, antigens, microorganisms, cells, tissues, nucleic acids and other biologically active substances), appropriate physical and chemical transducers (such as oxygen electrodes, photosensitive tubes, FETs, An analytical tool or system consisting of a piezoelectric crystal or the like and a signal amplifying device. Biosensors have the function of a receiver and a converter.

纳米技术引入生物传感器领域后，提高了生物传感器的灵敏度和其它性能，并促发了新型的生物传感器。因为具有了亚微米尺寸的换能器、探针或者纳米微系统，生物传感器的各种性能大幅提高。The introduction of nanotechnology into the field of biosensors has increased the sensitivity and other properties of biosensors and has led to new biosensors. Thanks to submicron-sized transducers, probes or nano-microsystems, the performance of biosensors has increased dramatically.

目前尚未有基于光电响应性半导体纳米材料的生物传感器。There are currently no biosensors based on photoelectrically responsive semiconductor nanomaterials.

发明内容Summary of the invention

本发明的目的是克服现有技术的不足之处，提供一种基于光电响应性半导体纳米材料的生物传感器。It is an object of the present invention to overcome the deficiencies of the prior art and to provide a biosensor based on a photoelectrically responsive semiconductor nanomaterial.

本发明的基于光电响应性半导体纳米材料的生物传感器包括分子识别部分和转换部分，所述的分子识别部分选用光电响应性半导体纳米材料，所述的光电响应性半导体纳米材料通过如下方法制备而成：The biosensor based on the photoelectric responsive semiconductor nanomaterial of the present invention comprises a molecular recognition portion and a conversion portion, wherein the molecular recognition portion is selected from a photoelectrically responsive semiconductor nano material, and the photoelectric responsive semiconductor nano material is prepared by the following method. :

1)以硅溶胶为原料制备二氧化硅晶体，再采用电子束蒸发的方式在金片表面得到纳米棒；1) preparing silica crystals from silica sol, and obtaining nanorods on the surface of the gold sheet by electron beam evaporation;

2)水热法制备纳米CdS颗粒，使用表面活性剂对纳米CdS颗粒表面进行改性处理；2) preparing nano-CdS particles by hydrothermal method, and modifying the surface of the nano-CdS particles by using a surfactant;

3)在纳米棒表面吸附纳米CdS颗粒，制备Si02-CdS光电响应性半导体纳 米材料。3) Adsorption of nano-CdS particles on the surface of nanorods to prepare SiO2-CdS photoelectrically responsive semiconductor nano Rice material.

优选地，Preferably,

所述的表面活性剂为聚乙二醇。The surfactant is polyethylene glycol.

本发明的基于光电响应性半导体纳米材料的生物传感器，灵敏度高、选择性好。The biosensor based on the photoelectric responsive semiconductor nano material of the invention has high sensitivity and good selectivity.

具体实施方式detailed description

实施例1Example 1

本发明的基于光电响应性半导体纳米材料的生物传感器包括分子识别部分和转换部分，所述的分子识别部分选用光电响应性半导体纳米材料，所述的光电响应性半导体纳米材料通过如下方法制备而成：The biosensor based on the photoelectric responsive semiconductor nanomaterial of the present invention comprises a molecular recognition portion and a conversion portion, wherein the molecular recognition portion is selected from a photoelectrically responsive semiconductor nano material, and the photoelectric responsive semiconductor nano material is prepared by the following method. :

1)以硅溶胶为原料制备二氧化硅晶体，再采用电子束蒸发的方式在金片表面得到纳米棒；1) preparing silica crystals from silica sol, and obtaining nanorods on the surface of the gold sheet by electron beam evaporation;

2)水热法制备纳米CdS颗粒，使用表面活性剂对纳米CdS颗粒表面进行改性处理；2) preparing nano-CdS particles by hydrothermal method, and modifying the surface of the nano-CdS particles by using a surfactant;

3)在纳米棒表面吸附纳米CdS颗粒，制备Si02-CdS光电响应性半导体纳米材料。3) Adsorption of nano-CdS particles on the surface of the nanorods to prepare SiO2-CdS photoelectrically responsive semiconductor nanomaterials.

所述的表面活性剂为聚乙二醇。 The surfactant is polyethylene glycol.

Claims (2)

基于光电响应性半导体纳米材料的生物传感器，包括分子识别部分和转换部分，其特征在于，所述的分子识别部分选用光电响应性半导体纳米材料，所述的光电响应性半导体纳米材料通过如下方法制备而成：A biosensor based on a photoelectrically responsive semiconductor nanomaterial, comprising a molecular recognition portion and a conversion portion, wherein the molecular recognition portion selects a photoelectrically responsive semiconductor nano material, and the photoelectric responsive semiconductor nano material is prepared by the following method Made of: 1)以硅溶胶为原料制备二氧化硅晶体，再采用电子束蒸发的方式在金片表面得到纳米棒；1) preparing silica crystals from silica sol, and obtaining nanorods on the surface of the gold sheet by electron beam evaporation; 2)水热法制备纳米CdS颗粒，使用表面活性剂对纳米CdS颗粒表面进行改性处理；2) preparing nano-CdS particles by hydrothermal method, and modifying the surface of the nano-CdS particles by using a surfactant; 3)在纳米棒表面吸附纳米CdS颗粒，制备Si02-CdS光电响应性半导体纳米材料。3) Adsorption of nano-CdS particles on the surface of the nanorods to prepare SiO2-CdS photoelectrically responsive semiconductor nanomaterials. 根据权利要求1所述的基于光电响应性半导体纳米材料的生物传感器，其特征在于，所述的表面活性剂为聚乙二醇。 The photoelectric sensor-based semiconductor nanomaterial-based biosensor according to claim 1, wherein the surfactant is polyethylene glycol.
PCT/CN2015/095041 2015-05-15 2015-11-19 Biosensor based on photoelectrically responsive semiconductor nanomaterial Ceased WO2016184062A1 (en)

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