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WO2014172852A1 - Facteur de transcription myb myb1-1 du coton et son gène codant, et son utilisation - Google Patents

Facteur de transcription myb myb1-1 du coton et son gène codant, et son utilisation Download PDF

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Publication number
WO2014172852A1
WO2014172852A1 PCT/CN2013/074590 CN2013074590W WO2014172852A1 WO 2014172852 A1 WO2014172852 A1 WO 2014172852A1 CN 2013074590 W CN2013074590 W CN 2013074590W WO 2014172852 A1 WO2014172852 A1 WO 2014172852A1
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plant
gene
seq
expression vector
recombinant
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陈文华
孙超
崔洪志
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BIOCENTURY TRANSGENE (CHINA) Co Ltd
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BIOCENTURY TRANSGENE (CHINA) Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Definitions

  • the present invention relates to MYB transcription factors and their coding genes and applications, in particular to a cotton-derived MYB transcription factor MYB1-1 and its coding gene, and its application in breeding drought-tolerant transgenic plants .
  • BACKGROUND OF THE INVENTION Abiotic stresses, such as drought, salt, extreme temperature, chemical pollution and oxygen damage, can cause serious damage to plant growth and development, causing great losses to crop yield, and the impact of drought on crop yield is It takes the first place in natural adversity, and its harm is equivalent to the sum of other disasters. It is the bottleneck of agricultural development in many areas.
  • the world's dry and semi-arid areas account for 34% of the land area; China's dry and semi-arid areas account for about 52% of the country's land area, and the annual drought area is 200 to 2.7 million hectares. 30 billion cubic meters, due to lack of water, less than 350 to 40 billion kilograms of grain; especially in China's major grain-producing areas such as North China, Northeast China and Northwest China, it is the region with the most water shortage in China, and the spring drought frequently reaches 10 years.
  • genes and their expression products can be divided into three categories: (1) genes and products involved in signal cascade amplification systems and transcriptional control; (2) genes and their expression products that directly contribute to the protection of biofilms and proteins; ) Proteins associated with the uptake and transport of water and ions.
  • genes and products involved in signal cascade amplification systems and transcriptional control (2) genes and their expression products that directly contribute to the protection of biofilms and proteins; ) Proteins associated with the uptake and transport of water and ions.
  • xerophytes and halophytes have yielded significant results for stress-related genes and signal transduction.
  • the system has a further understanding (Liu Q.1998.
  • Two transcription factors, DREB 1 and DREB2 with an EREBP/AP2 DNA binding domain, separate two cellular signal transduction pathways in drought and low temperature responsive gene expression, respectively, In Arabidopsis.
  • MYB1-1 a coding gene for a MYB transcription factor (designated herein as MYB1-1) of cotton using SSH (Suppression Subtractive Hybridization) in combination with RACE (Rapid Amplification of cDNA Ends). It was also found that when introduced into recipient plants, the drought tolerance of transgenic plants was significantly improved, and these traits were stably inherited.
  • the first aspect of the present invention provides a coding gene for a MYB-like transcription factor MYB1-1 of cotton (herein named G/z B - ), the sequence of which is SEQ ID NO: 2.
  • a second aspect of the present invention provides a recombinant expression vector comprising the gene of the first aspect of the present invention, which is obtained by inserting the gene into an expression vector, preferably, the expression vector is pCAMBIA2300; And the nucleotide sequence of the gene is operably linked to the expression control sequence of the recombinant expression vector; preferably, the recombinant expression vector is the rd29A-GhMYB 1-1-2300 vector shown in Figure 2.
  • a third aspect of the invention provides a recombinant cell comprising the gene of the first aspect of the invention or the recombinant expression vector of the second aspect of the invention; preferably, the recombinant cell is a recombinant Agrobacterium cell.
  • a fourth aspect of the present invention provides a method for improving drought tolerance of a plant, comprising: introducing the gene of the first aspect of the invention or the recombinant expression vector of the second aspect of the invention into a plant or plant tissue and causing the gene Expression;
  • the plant is tobacco.
  • a fifth aspect of the present invention provides a method for producing a transgenic plant, comprising: cultivating the gene comprising the first aspect of the present invention, the recombinant expression vector of the second aspect of the present invention under conditions effective to produce a plant A plant or plant tissue; preferably, the plant is tobacco.
  • a sixth aspect of the present invention provides the gene according to the first aspect of the present invention, the recombinant expression vector of the second aspect of the present invention or the recombinant cell of the third aspect of the present invention for improving drought tolerance of a plant and for use in plant breeding Use;
  • the plant is tobacco.
  • a seventh aspect of the invention provides the amino acid sequence encoded by the gene of the first aspect of the invention, as set forth in SEQ ID NO: 1.
  • Fig. 1 is a construction flow of a plant expression vector Crd29A-GhMYBl-1-23300 of G/z ZBl-1 (Fig. la-lb).
  • Figure 2 is a plasmid map of the plant expression vector (rd29A-GhMYBl-1-2300) of GhMYBW.
  • Figure 3 shows the results of drought tolerance simulation experiments of GhMYBl-1 transgenic tobacco T Q plants (T Q C 2 ; right, T Q C 7 ) and non-transgenic tobacco plants (left, CK) as controls.
  • Figure 4 shows the results of molecular level detection of the transcription level of the GhMYBl-1 gene in T Q transgenic tobacco plants and non-transgenic control plants by reverse transcription PCR.
  • M is Marker, 1-5 non-transgenic control tobacco plants, drought 6-18 significant effect in transgenic tobacco T Q generation plants to drought 19-24 no significant effect of T Q-generation transgenic tobacco plants.
  • Test materials A method according to PCR-select TM cDNA Clontech kit Subtraction Kit's instructions shown by suppression subtractive hybridization method for constructing subtracted library.
  • the mRNA of cotton seedling leaves treated with drought during the growth period was used as a tester during the experiment, and the mRNA of untreated cotton seedling leaves was used as a driver. Specific steps are as follows: (1) Test materials:
  • the test seedlings were divided into two groups, each with 4 pots and 1 pot per pot.
  • the first group was the control group, cultured at 25 ° C, light culture, normal 1/2MS watering;
  • the second group was drought treatment group, cultured at 25 ° C, photoperiod 16 hours light / 8 hours dark, cultured, stopped After watering, the leaves of the top 1/3 of the seedlings of the two groups were cut out in 10 days, frozen rapidly with liquid nitrogen, and stored in a -70 °C refrigerator.
  • the gene is not cleavable and the obtained sequence is in the untranslated region.
  • the second PCR product of the combined positive subtractive hybridization cDNA fragment (QIAquick PCR) Purification Kit purified, purchased from Qiagen) and pGEM-T Easy (purchased from Promega kit) vector, according to the method shown in the pGEM-T Easy kit product manual, the specific steps are as follows: Add the following ingredients in sequence using a 200 ⁇ PCR tube: The second PCR product of the purified forward subtractive hybridization cDNA fragment was 3 ⁇ l, ⁇ 4 ligase buffer 5 ⁇ l, pGEM-T Easy vector 1 ⁇ l, ⁇ 4 DNA ligase 1 ⁇ , and ligated overnight at 4 °C. Then 10 reaction products were added, added to 100 competent E.
  • coli JMI09 (purchased from TAKARA), ice bath for 30 min, heat shock for 60 s, ice bath for 2 min, and 250 ⁇ L LB liquid medium (containing 1% pancreas) Peptone (Tryptone, purchased from OXOID), 0.5% Yeast Extract (purchased from OXOID) and P 1% NaCl (purchased from Sinopharm) were placed in a 37 ° C shaker and shaken at 225 r/min.
  • LB liquid medium containing 1% pancreas
  • Peptone Teryptone, purchased from OXOID
  • 0.5% Yeast Extract purchased from OXOID
  • P 1% NaCl purchased from Sinopharm
  • the picked white colonies were inoculated into LB liquid medium containing 50 g/mL ampicillin in 96-well cell culture plate (CORNING), and cultured at 37 ° C overnight, and then glycerin was added to a final concentration of glycerol of 20% by volume. , and keep it at -80 °C.
  • the colony clones were cultured by nested PCR, Primer 1 and Primer 2R (PCR-selectTM cDNA Subtraction Kit from Clontech), and 292 positive clones were obtained, and all positive clones were obtained. Sended to Yingjie Jieji (Shanghai) Trading Co., Ltd. for sequencing.
  • GhMYBl-l GSP1 SEQ ID NO: 4:
  • GhMYBl-l GSP2 SEQ ID NO: 5:
  • GhMYBl-l GSP3 SEQ ID NO: 6:
  • the experimental procedure was performed according to the kit instructions (5' RACE System for Rapid Amplification of cDNA Ends kit purchased from Invitrogen).
  • the reverse-transcribed cDNA extracted from cotton leaves of the drought-treated group (reverse transcription primer SEQ ID NO: 4) was used as a template for the first round.
  • SEQ ID NO: 6 and the 3' primer AUAP for the second round of PCR amplification the specific steps are as follows: 50 ⁇ PCR reaction system: 5 ⁇ ⁇ ⁇ Buffer, 3 ⁇ 2.5 mM dNTP, 2.0 ⁇ diluted first round of PCR The product, 1.0 ⁇ Ex Taq, 10 ⁇ primers SEQ ID NO: 6 and AUAP each 2.0 ⁇ l, and 35 ⁇ double distilled water.
  • PCR reaction conditions pre-denaturation at 94 ° C for 5 min, denaturation at 94 ° C for 30 s, annealing at 58 ° C for 30 s,
  • the second PCR product recovery fragment was approximately 600 bp (Gel Extraction Kit was purchased from OMEGA) and ligated into pGEM-T Easy vector, transformed into JM109 (specific method as above), and 10 white colonies were randomly picked to contain 50 g/mL.
  • the ampicillin was cultured in LB liquid medium, and cultured at 37 ° C overnight, and then glycerin was added to a final concentration of glycerol of 20% by volume, and stored at -80 ° C until use.
  • SEQ ID NO: 6 and the 3' primer AUAP were used for PCR amplification (reaction system and reaction conditions are the same as above), and three positive clones were obtained, which were sent to Yingji Jieji (Shanghai) Trading Co., Ltd. for sequencing, and the cDNA of the gene was obtained. The 5' end. After the obtained 5' RACE product was cloned and sequenced, it was spliced with the cloned Gh-D178 sequencing result to obtain
  • a pair of primers were designed according to the sequence of SEQ ID NO: 17 as follows:
  • GhMYBl-rp SEQ ID NO: 7:
  • GhMYBl-l SEQ ID NO: 8: TCATTTCATTTCCAAACTTCTG
  • GhMYBW The full length of GhMYBW was cloned by SEQ ID NO: 7 and SEQ ID NO: 8.
  • PCR was performed using TaKaRa's PrimeSTAR HS DNA polymerase and cDNA from the reversed-treated mRNA extracted from cotton leaves of the drought-treated group.
  • 50 ⁇ PCR reaction system 10 ⁇ 5xPS Buffer, 3 ⁇ 2.5 mM dNTP, 2.0 ⁇ cDNA, 1.0 ⁇ PrimeSTAR, 10 ⁇ primer
  • SEQ ID NO: 7 and SEQ ID NO: 8 each of 2.0 ⁇ l, and 30 ⁇ M of double distilled water.
  • PCR amplification product plus A tail PCR product plus 2.5 volumes of absolute ethanol, -20 ° C for 10 minutes, centrifuge, remove the supernatant, air dry, dissolve with 21 ⁇ double distilled water, add 2.5 ⁇ ⁇ Buffer, 0.5 ⁇ l 5 ⁇ dATP, 2.5 ⁇ ⁇ Taq. Reaction conditions: The reaction was carried out at 70 ° C for 30 minutes. A DNA fragment of about 800 bp was recovered (Omega recovery kit), ligated into pGEM T-easy vector, transformed into JM109 (method as above), and 10 white colonies were randomly picked up in LB liquid containing 50 g/mL ampicillin.
  • the medium was cultured, cultured at 37 ° C overnight, and then glycerin was added to a final concentration of glycerol of 20% by volume, and stored at -80 ° C until use.
  • SEQ ID NO: 7 and SEQ ID NO: 8 were subjected to bacterial cell PCR amplification (reaction system and reaction conditions are the same as above), and 4 positive clones were obtained, which were sent to Yingjie Jieji (Shanghai) Trading Co., Ltd. for sequencing, and the sequence was SEQ ID. NO: 2.
  • amino acid sequence of the MYB1-1 protein encoded by the gene is shown in SEQ ID NO: 1.
  • Nucleotide sequence of the GhMYB ⁇ - ⁇ coding gene SEQ ID NO: 2 1 ATGGGAAGGT CTCCTTGCTG TGAGAAAGCT CATACAAACA AAGGTGCATG GACCAAAGAA
  • the plant binary expression vector pCAMBIA2300 (purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd.) was selected as a plant expression vector, and the 35S promoter of the ⁇ gene containing the double enhancer was replaced with the Pnos promoter to reduce the expression of prion protein in plants. .
  • Tnos acts as a promoter and terminator of the GhMYBl-1 gene.
  • Primer SEQ ID NO: 9 and SEQ ID NO: 10 were used to amplify Pnos using the plant expression vector pBI121 (purchased from Beijing Huaxia Ocean Technology Co., Ltd.) as a template, and TaKaRa's PrimeSTAR HS DNA polymerase was used. 50 ⁇ PCR reaction system: 10 ⁇ 5 xPS Buffer, 3 ⁇ 2.5 mM dNTP, 1.0 ⁇ PBI121, 1.0 ⁇ PrimeSTAR, 10 ⁇ primers SEQ ID ⁇ : 9 and SEQ ID NO: 10 each 2.0 ⁇ 1, and 31 ⁇ Double distilled water.
  • PCR reaction conditions pre-denaturation at 94 °C for 5 min, denaturation at 94 °C for 30 s, annealing at 56 °C for 30 s, extension at 72 °C for 30 s, after 33 cycles, extension at 72 °C for 10 min.
  • the resulting PCR product was digested with EcoRI, Bglll, and ligated into pCAMBIA2300 according to the kit instructions (Promega, T4 ligase kit) to obtain pCAMBIA2300-1.
  • PrimeSTAR HS DNA polymerase from TaKaRa 50 ⁇ PCR reaction system: 10 ⁇ 5 xPS Buffer, 3 ⁇ 2.5 mM dNTP, 1.0 ⁇ pBI121, 1.0 ⁇ PrimeSTAR, 10 ⁇ primer SEQ ID NO: 11 and SEQ ID NO: 12 each of 2.0 ⁇ l, and 31 ⁇ of double distilled water.
  • PCR reaction conditions predenaturation at 94 °C for 5 min, denaturation at 94 °C for 30 s, annealing at 58 °C for 30 s, extension at 72 °C for 30 s, after 33 cycles, extension at 72 °C for 10 min.
  • the resulting PCR product was digested with Sacl, EcoRI and ligated into pCAMBIA2300-1 according to the kit instructions (Promega, T4 ligase kit) to obtain pCAMBIA2300-2.
  • Arabidopsis thaliana Amplification of Arabidopsis thaliana with primers SEQ ID NO: 13 and SEQ ID NO: 14 using Arabidopsis thaliana (Columbia type, purchased from the Arabidopsis Bioresources Center (www.arabidopsis.org) of the Ohio State University) as a template rd29A promoter (see Zeng I, et L. 2002, Preparation of total DNA from "recalcit rant plant taxa", Acta Bot Sin, 44 (6):.. 694-697 extraction method Arabidopsis DNA) 0 using PrimeSTAR HS DNA polymerase from TaKaRa.
  • PCR reaction system 10 ⁇ 5 ⁇ PS Buffer, 3 ⁇ 2.5 mM dNTP, 1.0 ⁇ Arabidopsis DNA, 1.0 ⁇ PrimeSTAR, 10 ⁇ primers SEQ ID NO: 13 and SEQ ID NO: 14 each 2.0 ⁇ 1, and 31 ⁇ ⁇ double distilled water.
  • PCR reaction conditions predenaturation at 94 °C for 5 min, denaturation at 94 °C for 30 s, annealing at 58 °C for 30 s, extension at 72 °C for 30 s, after 33 cycles, extension at 72 °C for 10 min.
  • pCAMBIA2300-3 was obtained by restriction enzyme digestion with HindIII and Pstl (connection method as above) pCAMBIA2300-2.
  • GhMYBW (template is G/z ZB1-l obtained in Example 2) was amplified using primers SEQ ID NO: 15 and SEQ ID NO: 16, using TaKaRa's PrimeSTAR HS DNA polymerase.
  • PCR reaction conditions predenaturation at 94 °C for 5 min, denaturation at 94 °C for 30 s, annealing at 58 °C for 30 s, extension at 72 °C for 2 min, after 33 cycles, extension at 72 °C for 10 min.
  • the plant expression vector rd29A- was obtained by cleavage of Pstl and Sacl (connection method as above) pCAMBIA2300-3. GhMYB 1-1-2300.
  • Agrobacterium LBA4404 (purchased from Biovector Science Lab, Inc) Competent preparation: Agrobacterium LBA4404 was spotted on LB solid medium containing 50 g/ml rifampicin and 50 g/ml streptomycin 1-2 days in advance Inoculation, culture at 28 ° C for 1 to 2 days. Pick a single colony and inoculate it in 5 ml of LB liquid medium containing 50 ⁇ ⁇ / ⁇ 1 rifampicin and 50 ⁇ ⁇ / ⁇ 1 streptomycin, and incubate overnight at 28 °C (about 12-16 hours) to OD600 value. At 0.4, a seed bacterial liquid was formed.
  • Transformation of Agrobacterium The competent cells were thawed on ice, and 1 ⁇ of the rd29A-GhMYB 1-1-2300 plasmid obtained in Example 3 was added to 40 ⁇ of the competent cells, and the mixture was mixed and ice bathed for about 10 minutes. Transfer the mixture of competent and DNA to a pre-cooled electric shock cup with a micropipette and tap to bring the suspension to the bottom, taking care not to have air bubbles. Place the electric shock cup (purchased from bio-rad) on the slide of the electric shock chamber and push the slide to place the electric shock cup on the base electrode of the electric shock chamber.
  • the program of MicroPulser (purchased from Bio-Rad) is set to "Agr" and the electric shock is performed once. Immediately remove the electric shock cup and add the pre-warmed LB medium at 28 °C. Quickly and gently mix the competent cells with a micropipette. The suspension was transferred to a 1.5 ml centrifuge tube and incubated at 225 rpm for 1 hour at 28 °C.
  • Example 5 Agrobacterium-mediated leaf disc transformation was used to obtain transgenic tobacco
  • the leaves of the sterile seedlings were cut into 5 mm ⁇ 5 mm leaf discs, and the leaf discs were inoculated with Agrobacterium containing the expression vector rd29A-GhMYB 1-1-2300 in the logarithmic growth phase for 10 min, and the bacterial liquid was absorbed in the dark condition.
  • the cells were cultured for 2 days (MS medium).
  • the leaves were transferred to differentiation medium (MS+1 mg/L cytokinin (BA) + 0.1 mg/L naphthaleneacetic acid (NAA) + 50 mg/L kanamycin + 500 mg/L cephalosporin).
  • the obtained transgenic tobacco leaves were extracted, and the DNA was extracted (the Arabidopsis thaliana DNA extraction method in Example 3) using SEQ ID NO: 7 and SEQ ID NO: 8 (50 ⁇ PCR reaction system: 5 ⁇ ⁇ ⁇ Buffer, 3 ⁇ 2.5 mM dNTP, 2.0 ⁇ DNA, 1.0 ⁇ Ex Taq, 10 ⁇ primer SEQ ID NO: 9 and SEQ ID NO: 10 each 2.0 ⁇ l, and 35 ⁇ double distilled water.
  • SEQ ID NO: 7 and SEQ ID NO: 8 50 ⁇ PCR reaction system: 5 ⁇ ⁇ ⁇ Buffer, 3 ⁇ 2.5 mM dNTP, 2.0 ⁇ DNA, 1.0 ⁇ Ex Taq, 10 ⁇ primer SEQ ID NO: 9 and SEQ ID NO: 10 each 2.0 ⁇ l, and 35 ⁇ double distilled water.
  • PCR reaction conditions 94 ° C pre- Denaturation for 5 min, 94 denaturation for 30 s, annealing at 55 °C for 30 s, extension at 72 °C for 2 min, after 33 cycles, extension at 72 °C for 10 min), PCR identification, preservation of positive plants for numbering Tod-ToC ⁇
  • Example 6 Drought-tolerant simulation experiment and functional identification of overexpressed GhMYBl-1 transgenic tobacco T Q The sterilized vermiculite was soaked in 1/2 MS medium.
  • Tod-ToC ⁇ and control tobacco tissue culture seedlings were transplanted to vermiculite, respectively, at 25 °C, 10 hours light culture/14 hours dark culture cycle, 1/2MS every 5 days, 15 days after strong seedling culture, drought Stress test, transgenic tobacco, control tobacco drought for 14 days (without watering), 25 °C, 10 hours light culture / 14 hours dark culture cycle.
  • the drought resistance of T0 transgenic plants showed that the control plants were wilting, while T Q C 2 , T 0 C 3 ToC 7 T 0 Cio T 0 Cii T Q C 14 of the six strains had 21 of 29 tobaccos. It can grow normally and exhibits significant drought tolerance (see Figure 3, taking ToC 2 and ToC 7 as examples, T. C 3 , T 0 Cio T. C U , 1 ). 14 results with T. C 2 , T. C 7 is similar, not shown here).
  • RNA extraction kit (Invitrogen). The absorbance values of total RNA at 260 nm and 280 nm were measured using a HITACHI UV spectrophotometer U-2001 to calculate the respective RNA concentrations. Reverse transcription was carried out according to the method shown by Invitrogen reverse transcription assay LIBOX Superscript III Reverse Transcriptase (.2 ⁇ ag total RNA as a template, reverse transcription primer SEQ ID NO: 8).
  • the relative expression of MYBl-1 protein was detected by amplifying GhMYBW by SEQ ID NO: 7 and SEQ ID NO: 8.
  • the PCR reaction was carried out using reverse-transcribed cDNA as a template using TaKaRa's PrimeSTAR HS DNA polymerase.
  • 50 ⁇ PCR reaction system 10 ⁇ 5 ⁇ PS Buffer, 3 ⁇ 2.5 mM dNTP, 2.0 ⁇ cDNA, 1.0 ⁇ PrimeSTAR, 10 ⁇ primers SEQ ID NO: 7 and SEQ ID NO: 8 each 2.0 ⁇ l, and 30 ⁇ double Steamed water.
  • M is DNA Ladder Marker (DL2000, purchased from Shenzhen Ruizhen Biotechnology Co., Ltd.), 1-5 is control tobacco, and 6-18 is drought-tolerant transgenic tobacco T Q- generation plants, 19 -24 is drought-tolerant and not significantly transgenic tobacco T Q plants.
  • the strip size shown in the figure is the same as the size of GhMYB ⁇ - ⁇ (about 800bp).

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Abstract

La présente invention concerne un facteur de transcription MYB MYB1-1 dérivé du coton et un gène codant pour ledit facteur, ainsi que son utilisation dans la culture de plantes transgéniques présentant une tolérance supérieure à la sécheresse.
PCT/CN2013/074590 2013-04-24 2013-04-24 Facteur de transcription myb myb1-1 du coton et son gène codant, et son utilisation Ceased WO2014172852A1 (fr)

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CN201380074522.XA CN105073772A (zh) 2013-04-24 2013-04-24 一个棉花myb类转录因子myb1-1及其编码基因与应用
PCT/CN2013/074590 WO2014172852A1 (fr) 2013-04-24 2013-04-24 Facteur de transcription myb myb1-1 du coton et son gène codant, et son utilisation

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CN106892973A (zh) * 2017-01-12 2017-06-27 淮阴工学院 植物抗逆性相关蛋白GhMYB4及编码基因与应用
CN109295069B (zh) * 2018-09-19 2021-08-20 昆明理工大学 珠子参转录因子基因PjMYB1的应用
CN111961123B (zh) * 2020-07-09 2022-04-08 华中农业大学 玫瑰RrMYB18转录因子及其促进植物次生壁生物合成和植株矮化中的应用
CN112280787B (zh) * 2020-11-10 2021-09-21 中国科学院华南植物园 一种甘草myb1基因、其编码的蛋白和应用
CN116217683B (zh) * 2022-09-08 2024-04-16 深圳全棉时代科技有限公司 一种与棉花纤维品质相关的基因、超表达载体和敲除载体及应用

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