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WO2014088242A1 - Dispositif de production d'eau à microbulles utilisant un vibreur à ultrasons, un milieu de culture de cellules contenant de l'eau à microbulles, un procédé de culture de cellules l'utilisant, un carburant mélangé à haute efficacité utilisant des microbulles et procédé de fabrication associé - Google Patents

Dispositif de production d'eau à microbulles utilisant un vibreur à ultrasons, un milieu de culture de cellules contenant de l'eau à microbulles, un procédé de culture de cellules l'utilisant, un carburant mélangé à haute efficacité utilisant des microbulles et procédé de fabrication associé Download PDF

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Publication number
WO2014088242A1
WO2014088242A1 PCT/KR2013/010476 KR2013010476W WO2014088242A1 WO 2014088242 A1 WO2014088242 A1 WO 2014088242A1 KR 2013010476 W KR2013010476 W KR 2013010476W WO 2014088242 A1 WO2014088242 A1 WO 2014088242A1
Authority
WO
WIPO (PCT)
Prior art keywords
kpa
gas
porous tube
microbubble
ultrasonic vibrator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2013/010476
Other languages
English (en)
Korean (ko)
Inventor
김종민
오승훈
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Industry Academic Cooperation Foundation of Chung Ang University
Original Assignee
Industry Academic Cooperation Foundation of Chung Ang University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020130038064A external-priority patent/KR101505917B1/ko
Application filed by Industry Academic Cooperation Foundation of Chung Ang University filed Critical Industry Academic Cooperation Foundation of Chung Ang University
Priority to CN201380072289.1A priority Critical patent/CN104968607A/zh
Priority to US14/649,401 priority patent/US9908089B2/en
Priority claimed from KR1020130140180A external-priority patent/KR101455115B1/ko
Priority claimed from KR1020130140181A external-priority patent/KR101588246B1/ko
Publication of WO2014088242A1 publication Critical patent/WO2014088242A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/34—Treatment of water, waste water, or sewage with mechanical oscillations
    • C02F1/36—Treatment of water, waste water, or sewage with mechanical oscillations ultrasonic vibrations
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/32—Liquid carbonaceous fuels consisting of coal-oil suspensions or aqueous emulsions or oil emulsions
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L2290/00—Fuel preparation or upgrading, processes or apparatus therefore, comprising specific process steps or apparatus units
    • C10L2290/34—Applying ultrasonic energy

Definitions

  • the present application is a microbubble water production apparatus using an ultrasonic vibrator capable of mass production of the bubble water maximized dissolved gas, microbubble release member, cell culture medium containing microbubble water satisfying both economical and safety and cell culture method using the same And a high efficiency mixed fuel using fine bubbles and a manufacturing apparatus thereof.
  • gas water production method and manufacturing apparatus used a method of increasing the dissolved gas amount while mixing the water at a high speed by using a motor, the method is difficult to obtain the desired dissolved gas amount in a short time as well as fine bubbles It is difficult to produce.
  • oxygen (gas) water by electrolysis or refrigeration, but in this case, the equipment is very expensive and the mixed oxygen (gas) is present in the water in a bubble state, and the dissolved oxygen amount rapidly with time.
  • the method of preparing oxygen (gas) water by supplying fine air bubbles has the advantage of producing a high concentration of oxygen (gas) water, but in order to supply the nanobubble to continuously supply the nanobubble under high pressure conditions It must be produced and supplied, and this also has a disadvantage that a large amount of oxygen (gas) is present in the bubble state in the water, and the dissolved oxygen amount drops quickly over time.
  • Serum is a complex product mixed with various substances and is used as an additive in a basic culture medium in a cell culture chamber, and contains growth factors, hormones, and components that stimulate cells in cell culture.
  • FBS fetal bovine serum
  • the fetal bovine serum is serum isolated from bovine blood during pregnancy, especially in animal cell culture, which is a fundamental step in biotechnology-related experiments, as well as vaccines, protein medicines and treatments that have become increasingly fast worldwide in recent years. It is a raw material used for development, such as an antibody.
  • 10-0394430 discloses a method for culturing a human cell comprising human serum in a medium used for culturing a human cell.
  • the patent can be infected with human viruses, such as AIDS, and the like, there is a limit to the supply, the effect is not great in other animal cell culture, the effect is significantly lower than the medium using fetal bovine serum, even in human cell culture, fetal bovine serum Cannot be replaced completely.
  • the use of fetal bovine serum can be reduced somewhat, but there is a problem that the economic efficiency is also greatly reduced.
  • the use of expensive fetal bovine serum was reduced by containing nanobubble water as a component in cell culture.
  • 10-1071461 relates to a microbubble generating device, and discloses a microbubble generating device for converting a mixed oil mixed with water and a fuel into an emulsion state.
  • the above-described method has a problem that the emulsion state may become unstable and the separation of water and fuel may occur when a predetermined time has elapsed in the mixed oil converted into the emulsion state.
  • An object of the present application is to receive the ultrasonic vibration propagated from the ultrasonic vibrator to vibrate the porous tube to release the gas into the micro-bubble, when generating the micro-bubble from the porous tube, it is easy to aggregate between the micro-bubbles and desorption of the micro-bubble and the porous tube.
  • An object of the present invention is to provide a microbubble water manufacturing apparatus using an ultrasonic vibrator capable of producing a large amount of bubble water in a relatively short time.
  • Another object of the present invention is to provide a microbubble emitting member, which is applied to the porous tube by ultrasonic vibration generated in the ultrasonic vibrator to facilitate the desorption of the micro bubbles generated in the porous tube.
  • Still another object of the present invention is to provide a highly efficient mixed fuel using a microbubble, and a manufacturing apparatus thereof.
  • a first aspect of the present disclosure is a liquid tank containing a liquid; A liquid circulation line unit for forcibly circulating a liquid contained in the liquid tank; A gas supply line unit supplying gas to the liquid tank; And installed inside the liquid tank in a state of being connected to the gas supply line part, and when the gas supplied from the gas supply line part is discharged into the micro bubbles, the micro bubbles are vibrated by ultrasonic waves so as not to aggregate with each other.
  • a microbubble water production apparatus using an ultrasonic vibrator comprising a plurality of microbubble emitting member to shake off the micro-bubbles.
  • the second side of the present application is provided to be connected to one side of the porous tube, the body portion is generated vibration by the ultrasonic vibrator provided therein; And a vibration transmitting member attached to one side of the porous tube to transfer the ultrasonic vibration generated from the body to the porous tube, wherein the ultrasonic vibration is applied to the porous tube through the vibration transmitting member. It provides a microbubble discharge member that is to desorb the micro-bubbles generated in the tube.
  • a cell culture medium comprising serum and antibiotic, wherein the cell culture medium contains microbubble water, and about 10 3 to about 10 18 about 1 mL of about 1 mL of the microbubble water. It provides a cell culture medium, comprising a microbubble having an average diameter of nm to about 1,000 ⁇ m.
  • a fourth aspect of the present disclosure provides a method of culturing cells comprising culturing the cells confluent in the culture medium; And replacing the culture medium of the cells with a culture medium containing microbubble water, wherein the average of about 10 3 to about 10 18 about 1 nm to about 1,000 ⁇ m per about 1 mL of the microbubble water. It provides a cell culture method comprising a microbubble having a diameter.
  • a fifth aspect of the present application fuel; And a fine bubble formed in the fuel.
  • a sixth aspect of the present disclosure includes a liquid tank into which a liquid is injected; A gas supply line unit supplying gas to the liquid tank; And it provides a high-efficiency mixed fuel manufacturing apparatus comprising a porous tube installed inside the liquid tank.
  • the apparatus for manufacturing microbubble water using the ultrasonic vibrator according to the present invention firstly vibrates by receiving ultrasonic vibration propagated from the ultrasonic vibrator through a porous tube in which gas of low pressure supplied from the outside is discharged into fine bubbles, and the microbubble is a porous tube.
  • Bubble water can be prepared.
  • very small and relatively uniform microbubbles are generated so that the microbubbles are efficiently dissolved in the liquid (water), a predetermined dissolved gas ratio of bubble water can be achieved in a relatively short time.
  • ultrasonic vibration generated in an ultrasonic vibrator may be applied to a porous tube to facilitate desorption of microbubbles generated in the porous tube.
  • the cell culture medium according to the present invention can reduce the amount of expensive fetal bovine serum by including microbubble water, and exhibit the same or more cell growth promoting effect as the culture medium containing fetal bovine serum, and the microbubble water itself is safe. Because of this, there is an advantage that can be cultured cells economically and safely.
  • the high-efficiency mixed fuel and the high-efficiency mixed fuel manufacturing apparatus by forming a microbubble in the existing fuel has the effect of combustion of fuel, fuel efficiency, further energy saving, and harmful emissions.
  • the microbubbles included in the fuel may improve the efficiency of the fuel by reducing the friction force generated between the inner surface of the pipeline and the fuel when the fuel passes through the pipeline, the amount of harmful emissions generated after combustion of the fuel Can be reduced to a significant level.
  • Figure 1 shows a microbubble water generating device according to an embodiment of the present application.
  • Figure 2 is a cross-sectional view showing a microbubble emitting member according to an embodiment of the present application.
  • Figure 3 is an exemplary view showing an operating state of the microbubble emitting member according to an embodiment of the present application.
  • 4A and 4B are bubble size and concentration measurement test tables of hydrogen microbubble water according to an embodiment of the present application.
  • 5a and 5b is a bubble size and concentration measurement test table of the oxygen microbubble water according to an embodiment of the present application.
  • FIG. 6 is an analysis of the growth of lung cancer cells A549 cultured at various times in a cell culture medium containing hydrogen microbubbles and oxygen microbubbles according to an embodiment of the present disclosure using optical image cell counting.
  • FIG. 7 is a cell image of lung cancer cell A549 according to an embodiment of the present application.
  • FIG. 8 is an analysis of growth of lung cancer cells A549D9K cultured at various times in a cell culture medium containing hydrogen microbubbles and oxygen microbubbles according to an embodiment of the present disclosure using optical image cell counting.
  • FIG. 9 is a cell image of lung cancer cell A549D9K according to an embodiment of the present application.
  • Figure 11 analyzes the cell image of osteoblast MC3T3 according to an embodiment of the present application.
  • Figure 13 analyzes the cell image of the fibroblast NIH3T3 according to an embodiment of the present application.
  • kidney cells HEK293 are cell images of kidney cells HEK293 according to an embodiment of the present application.
  • FIG. 16 is a high sensitivity charge coupled device (CCD) camera and an X20 including a nano sight LM10-HS and LM14 having a 405 nm laser after 121 days of microbubble formed gasoline according to an embodiment of the present disclosure. It is an image of microbubbles taken using a microscope objective lens.
  • CCD charge coupled device
  • Figure 17a shows the change in the number of microbubble population over time of the gasoline with a microbubble formed in accordance with an embodiment of the present application
  • Figures 17b to 17d is after the formation of each microbubble gasoline, after 76 days, And size distribution of the microbubbles after 121 days.
  • Figure 18a shows the viscosity of the existing gasoline and gasoline formed microbubble according to an embodiment of the present application
  • Figure 18b shows the surface tension of the existing gasoline and gasoline formed microbubble according to an embodiment of the present application.
  • FIG. 19 is a structural diagram showing an apparatus for manufacturing a high efficiency mixed fuel using a microbubble according to an embodiment of the present application.
  • 20 is a schematic diagram showing the generation of microbubbles on the surface of the porous material according to an embodiment of the present application.
  • 21A to 21C illustrate power characteristics of a conventional gasoline and a gasoline in which a microbubble is formed according to an embodiment of the present disclosure.
  • 22a to 22d show the generation rate of harmful exhaust emissions according to the engine load of the existing gasoline and gasoline with a microbubble formed according to an embodiment of the present application.
  • microbubbles shall include microbubbles of micrometer size and / or nanobubbles of nanometer size, and the size of the microbubbles may be those having an average diameter of about 1 nm to about 1,000 ⁇ m. However, this may not be limited. For example, the size of the microbubbles may have an average diameter of about 1 ⁇ m to about 1,000 ⁇ m, and the size of the nanobubbles may have an average diameter of about 1 nm to about 1,000 nm.
  • gases used to form microbubbles include hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and It may include a gas selected from the group consisting of combinations thereof, but may not be limited thereto.
  • the nitrogen-containing compound gas is not particularly limited as long as it is in a gaseous state as a compound containing nitrogen.
  • the nitrogen-containing compound gas may include, but may not be limited to.
  • the carbon-containing compound gas is not particularly limited as long as it is a compound containing carbon and is in a gaseous state.
  • the carbon-containing compound gas may include a hydrocarbon compound gas having 1 to 4 carbon atoms (methane, ethane, propane, butane, etc.). This may not be limited.
  • a first aspect of the present disclosure is a liquid tank containing a liquid; A liquid circulation line unit for forcibly circulating a liquid contained in the liquid tank; A gas supply line unit supplying gas to the liquid tank; And installed inside the liquid tank in a state of being connected to the gas supply line part, and when the gas supplied from the gas supply line part is discharged into the micro bubbles, the micro bubbles are vibrated by ultrasonic waves so as not to aggregate with each other.
  • a microbubble water production apparatus using an ultrasonic vibrator comprising a plurality of microbubble emitting member to shake off the micro-bubbles.
  • the liquid may include one selected from the group consisting of water, a high viscosity material, and combinations thereof, but may not be limited thereto.
  • the high viscosity material may be selected from the group consisting of polymer, fuel, and combinations thereof, but may not be limited thereto.
  • the high viscosity material may include one selected from the group consisting of polymers, fossil fuels, biofuels, and combinations thereof, for example, lubricating oil, gasoline, diesel, bunker oil, bioethanol, biomethanol , Biodiesel, and combinations thereof, but may not be limited thereto.
  • the apparatus for manufacturing microbubble water using the ultrasonic vibrator has a predetermined space formed therein such that the liquid tank 100 receives a predetermined amount of liquid.
  • a viewing window (not shown) capable of confirming the inside may be formed, but may not be limited thereto.
  • An upper surface of the liquid tank 100 may be provided with a check valve (not shown) for checking the pressure in the liquid tank 100 and adjusting the pressure in the liquid tank 100, but is not limited thereto. You may not.
  • an outlet 101 capable of discharging the liquid contained in the liquid tank 100 and an inlet 102 capable of introducing liquid into the liquid tank 100 may be formed, but may not be limited thereto.
  • the outlet 101 may be formed at a position lower than the level of the liquid contained in the liquid tank 100, but may be formed below the liquid tank 100, but is not limited thereto.
  • the inlet 102 may include an upper portion or a lower portion of the liquid tank 100, but may not be limited thereto.
  • a gas injection member through which gas is introduced may be additionally installed on the upper portion of the liquid tank 100, but may not be limited thereto.
  • the gas injection member may include injecting gas into the liquid tank 100 to maintain an atmosphere in the liquid tank 100 at a gas saturation state, and may generate pressure in the liquid tank 100. This may not be limited.
  • the liquid circulation line unit 200 circulates the liquid contained in the liquid tank 100, but may include a circulation tube 210 and a circulation motor 220.
  • the circulation pipe 210 connects the discharge port 101 and the inlet port 102 provided in the liquid tank 100 to discharge the liquid in the liquid tank 100 through the discharge port 101. After that, it may be to be re-introduced into the liquid tank 100 through the inlet 102 to circulate, but may not be limited thereto.
  • the circulation tube 210 is provided with the circulation motor 220 and is selectively driven according to a control signal applied from the outside, so that the liquid in the liquid tank 100 discharged through the discharge port 101 is discharged.
  • the liquid circulation line unit 200 the circulation pipe 210 is connected to the outlet 101 and the inlet port 102 of the liquid tank 100 in accordance with a control signal applied from the outside of the circulation pipe 210
  • the circulation motor 220 is driven to the liquid in the liquid tank 100 is discharged through the discharge port 101, circulated along the circulation pipe 210 to the back through the inlet 102 It may be to circulate to be drawn into the liquid tank 100, but may not be limited thereto.
  • the gas supply line unit 300 supplies gas to the liquid tank 100, and a gas cylinder 310, a supply pipe 320, a pressure control valve 330, and a minute It may include a pipe 340, but may not be limited thereto.
  • the gas is selected from the group consisting of hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof It may be to include, but may not be limited thereto.
  • the gas cylinder 310 of the gas supply line 300 is filled with the gas to be dissolved in the liquid tank 100, the gas may be supplied to the internal pressure in the cylinder generated in the gas filled state, but is not limited thereto. It may not be.
  • the gas cylinder 310 may also include a pressure gauge and an opening / closing valve for checking pressure, but may not be limited thereto.
  • the opening and closing valve (not shown) of the gas cylinder 310 may be connected to the supply pipe 320 so that the gas supplied from the gas cylinder 310 may be guided, but may not be limited thereto.
  • the supply pipe 320 is connected to the pressure control valve 330, the pressure of the gas guided through the supply pipe 320 is selectively reduced or pressurized by the pressure control valve 330 to a predetermined uniform
  • the pressure may be adjusted to supply gas, but may not be limited thereto.
  • the distribution pipe 340 may be provided to distribute the reduced pressure or pressurized gas through the pressure control valve 330, but may not be limited thereto.
  • the gas is injected into the liquid tank 100 through the gas injection member provided on the upper portion of the liquid tank 100 through the distribution pipe 340, or through the distribution pipe 340.
  • the gas may be supplied to the microbubble discharge member 400 installed inside the liquid tank 100, but may not be limited thereto.
  • the gas supply line unit 300 is the gas filled in the gas cylinder 310 is supplied by the internal pressure of the cylinder, guided by the supply pipe 320 is a predetermined uniformity by the pressure control valve 330 It may include being distributed by the distribution pipe 340 while maintaining a pressure, the gas of the gas cylinder 310 may be supplied to the gas injection member and the fine bubble discharge member 400, This may not be limited.
  • the microbubble discharging member 400 is installed in the lower side of the liquid tank 100 in a state connected to the gas supply line part 300, and the distribution pipe 340.
  • the gas injected through the microbubble discharge member 400 may be discharged as a fine bubble, but may not be limited thereto.
  • the fine bubble discharge member 400 is preferably installed at a position lower than the level of the liquid contained in the liquid tank (100).
  • the average diameter of the micro bubbles emitted through the microbubble emitting member 400 may be about 1 nm to about 1,000 ⁇ m, but may not be limited thereto.
  • the average diameter of the microbubbles is about 1 nm to about 1,000 ⁇ m, about 10 nm to about 1,000 ⁇ m, about 100 nm to about 1,000 ⁇ m, about 300 nm to about 1,000 ⁇ m, about 500 nm to about 1,000 ⁇ m , About 700 nm to about 1,000 ⁇ m, about 900 nm to about 1,000 ⁇ m, about 1 ⁇ m to about 1,000 ⁇ m, about 10 ⁇ m to about 1,000 ⁇ m, about 100 ⁇ m to about 1,000 ⁇ m, about 300 ⁇ m to about 1,000 ⁇ m, about 500 ⁇ m to about 1,000 ⁇ m, about 700 ⁇ m to about 1,000 ⁇ m, about 900 ⁇ m to about 1,000 ⁇ m, about 1 nm to about 900 ⁇ m, about 10 nm.
  • the microbubble discharge member is a porous tube 410
  • the porous tube body 410 is formed with the fine pores communicating with the inside so that the gas injected through the distribution pipe 340 is discharged into a fine bubble
  • the porous tube body 410 It is provided to be connected to one side of, the body portion 420 that is generated by the vibration generated by the ultrasonic vibrator 426 provided therein, and attached to one side of the porous tube 410 in the body portion 420 It may include a vibration transmission member 430 for transmitting the ultrasonic wave propagated to the porous tube 410, but may not be limited thereto.
  • the porous tube 410 in which the fine pores are formed may have a hole of about 1 nm to about 1 mm, but may not be limited thereto.
  • the porous tube has about 1 nm to about 1 mm, about 10 nm to about 1 mm, about 100 nm to about 1 mm, about 300 nm to about 1 mm, about 500 nm to about 1 mm, about 700 Nm to about 1 mm, about 900 nm to about 1 mm, about 1 ⁇ m to about 1 mm, about 10 ⁇ m to about 1 mm, about 100 ⁇ m to about 1 mm, about 300 ⁇ m to about 1 mm, about 500 ⁇ m to About 1 mm, about 700 ⁇ m to about 1 mm, about 900 ⁇ m to about 1 mm, about 1 nm to about 900 ⁇ m, about 10 nm to about 900 ⁇ m, about 100 nm to about 900 ⁇ m, about 300 nm to
  • the porous tube 410 may be further provided with a front closing cap 440 and a rear closing cap 450, but may not be limited thereto.
  • the front closure cap 440 is formed on the front end of the porous tube 410 so that the coupling nut portion 441 is formed on one side so that the coupling nut portion 441 is accommodated inside the front end side of the porous tube 410.
  • Is coupled in this case may be to be coupled in a state in which a sealing ring (not shown) is located between the porous tube 410 and the front closing cap 440 to maintain the airtightness, but may not be limited thereto.
  • the rear closing cap 450 is formed with a bolt portion 451 on one side, and enters from the rear end side of the porous tube 410 and fastened with the coupling nut portion 441 of the front closing cap 440. To be coupled to the rear end side of the porous tube 410, but may not be limited thereto. At this time, the rear closing cap 450 may also be coupled between the porous tube 410 and the rear closing cap 450 in a state in which a sealing ring (not shown) is positioned to maintain airtightness. .
  • the rear closing cap 450 has a plurality of gas through holes 452 are formed around the bolt 451 around the bolt 451, and the gas introduced through the gas through hole 452 is porous.
  • the curved groove 453 is formed along the outer circumferential surface of the rear closing cap 450, the rear closing cap 450 having the curved groove 453 may be connected to the front end of the body portion 420, This may not be limited.
  • Vibration transmission member 430 is attached to the outside of the rear closing cap 450 of the porous tube 410 may include transmitting the ultrasonic vibration generated in the body portion 420 to the porous tube 410, but However, this may not be limited.
  • the vibration transmission member 430 may be a vibration pin to be vibrated by the ultrasonic wave toward the outward ultrasonic vibrator 426, the ultrasonic vibration is transmitted to the porous tube 410 by the vibration pin.
  • this may not be limited.
  • the body portion 420 may include a main body tube 421, an elastic fixing ring 422, and a connecting tube 423, but may not be limited thereto.
  • the main body tube 421 is formed in a tubular shape having a predetermined diameter, the front end inner peripheral surface is formed with a curved coupling groove 424 when the rear end of the porous tube 410 is connected to the front end of the body portion 420 the rear
  • the curved groove 453 of the closing cap 450 and the curved coupling groove 424 of the main tube 421 may be replaced with each other, but may not be limited thereto.
  • the elastic fixing ring 422 is provided between the curved groove 453 of the rear closing cap 450 and the curved coupling groove 424 of the main body tube 421 facing each other, the body portion It may include, but is not limited to, the porous tube 410 to be fixed to the flow (420).
  • the elastic fixing ring 422 may be to maintain the airtight between the porous tube 410 and the body 420, as well as fixing the porous tube 410 and the body portion 420.
  • the rear end of the main body tube 421 is connected to the connecting pipe 423, the center of the connecting pipe 423 is provided with an ultrasonic vibrator 426, around the ultrasonic vibrator 426 around the A gas inlet hole 425 connected to the distribution pipe 340 of the gas supply line part 300 may be formed, but may not be limited thereto.
  • the ultrasonic vibrator 426 is the same as a conventional ultrasonic vibrator, a detailed description thereof may be omitted and may be selectively driven by an external control, but may not be limited thereto.
  • the frequency of the ultrasonic vibrator may be about 1 Hz to about 300 MHz, but may not be limited thereto.
  • the frequency of the ultrasonic vibrator is about 1 Hz to about 300 MHz, about 10 Hz to about 300 MHz, about 100 Hz to about 300 MHz, about 300 Hz to about 300 MHz, about 500 Hz to about 300 MHz, About 700 Hz to about 300 MHz, about 900 Hz to about 300 MHz, about 1 Hz to about 300 MHz, about 10 Hz to about 300 MHz, about 100 Hz to about 300 MHz, about 300 Hz to about 300 MHz, about 500 KHz to about 300 MHz, about 700 Hz to about 300 MHz, about 900 Hz to about 300 MHz, about 1 MHz to about 300 MHz, about 10 MHz to about 300 MHz, about 100 MHz to about 300 MHz, about 1 Hz to About 100 MHz, about 10 Hz to about 100 MHz, about 100 Hz to about 100 MHz, about 300 Hz to about 100 MHz, about 500 Hz to about 100 MHz, about 700 Hz to about 100 MHz, about 900 Hz to about 100 MHz, about
  • the liquid tank 100 may further include a heating device or a cooling device, but may not be limited thereto.
  • the heating device or the cooling device may include being installed inside or outside the liquid tank 100, but may not be limited thereto.
  • the liquid tank 100 further includes the heating device, for example, in the manufacture of the microbubbles, high viscosity materials may be difficult to produce microbubbles due to their viscosity.
  • a heating device in the liquid tank, it is possible to generate a microbubble by lowering the viscosity of the high viscosity material to an appropriate level through temperature control.
  • the heating device may include a heating coil using resistance heat outside the liquid tank, and heat the high viscosity material at an appropriate temperature by flowing a current, but may not be limited thereto.
  • the high viscosity material may be selected from the group consisting of fossil fuels, biofuels, polymers, and combinations thereof, but may not be limited thereto.
  • the high viscosity material may be selected from the group consisting of lubricating oil, polymer, gasoline, diesel, bunker oil, bio ethanol, bio methanol, bio diesel, and combinations thereof, but may not be limited thereto.
  • lubrication characteristics can be improved by generating a fine bubble in the lubricating oil.
  • a method of lowering the viscosity of the lubricating oil through temperature control of the lubricating oil was used. The above method adversely affects the oil film formation of the lubricating oil, resulting in a decrease in the lubricating characteristic.
  • the lubricating oil in which the microbubbles are generated can be smoothly moved compared to the lubricating oil which is not, thereby reducing friction.
  • the wall slips while maintaining the viscosity necessary for forming the oil film through the lubricating oil including the microbubbles.
  • the fuel may be continuously evaporated during the microbubble generation process due to the volatility of the fuel. have.
  • a cooling device may be installed in the liquid tank, or a microbubble may be first generated in the fuel and then the additive may be mixed to lower the volatility of the fuel, but the present invention may not be limited thereto.
  • the fuel may include, but is not limited to, one selected from the group consisting of fossil fuels, biofuels, and combinations thereof.
  • the fossil fuel may include, but is not limited to, one selected from the group consisting of gasoline, diesel, lubricating oil, bunker oil, and combinations thereof.
  • the biofuel may include one selected from the group consisting of bioethanol, biomethanol, biodiesel, and combinations thereof, but may not be limited thereto.
  • the cooling device When the cooling device is installed in the liquid tank, for example, the cooling device may include being installed inside or outside the liquid tank, but may not be limited thereto.
  • the cooling device may be cooled by a refrigerant circulated through a cooling channel, and the refrigerant may be, for example, air cooling using cold air, but may not be limited thereto.
  • the volatility of the fuel may be lowered by a method of mixing an additive, but may not be limited thereto. The volatility of the fuel can be determined not only by the properties of the fuel but also by the additives added to the fuel.
  • volatility when the fuel is gasoline, volatility may be determined according to the mixing of butane, which is dissolved in gasoline and contributes to controlling the vapor pressure of the gasoline and improving octane number. Therefore, after the fine bubble is mixed before butane is mixed, butane may be dissolved to prevent a problem due to volatility of the fuel, but may not be limited thereto.
  • the porous tube 410 in which the gas supplied from the outside is discharged as a fine bubble is received and vibrated by the ultrasonic vibration propagated from the ultrasonic vibrator 426, so that the micro bubbles are formed in the porous tube 410.
  • Bubble water can be produced in which the amount of dissolved bubbles of the liquid contained in the liquid tank 100 is maximized by adhering to the surface or desorbed from the porous tube 410 before being agglomerated with each other.
  • the production cost can be drastically reduced by enabling mass production with relatively simple equipment without the need.
  • Residual gas that is not mixed with the liquid is allowed to remain in the liquid tank 100 to maintain the atmosphere in the liquid tank 100 in gas saturation, thereby maximizing the amount of dissolved gas in the bubble water, and is sufficient even over time
  • the amount of dissolved gas can be maintained.
  • the micro-bubble can be efficiently dissolved in the liquid by forming a very uniform and relatively uniform microbubble, it is possible to achieve the dissolved gas ratio of the bubble water in a relatively short time.
  • the second side of the present application is provided to be connected to one side of the porous tube, the body portion is generated vibration by the ultrasonic vibrator provided therein; And a vibration transmitting member attached to one side of the porous tube to transfer the ultrasonic vibration generated from the body to the porous tube, wherein the ultrasonic vibration is applied to the porous tube through the vibration transmitting member. It provides a microbubble discharge member that is to desorb the micro-bubbles generated in the tube.
  • the porous tube may have a hole of about 1 nm to about 1 mm, but may not be limited thereto.
  • the porous tube has about 1 nm to about 1 mm, about 10 nm to about 1 mm, about 100 nm to about 1 mm, about 300 nm to about 1 mm, about 500 nm to about 1 mm, about 700 Nm to about 1 mm, about 900 nm to about 1 mm, about 1 ⁇ m to about 1 mm, about 10 ⁇ m to about 1 mm, about 100 ⁇ m to about 1 mm, about 300 ⁇ m to about 1 mm, about 500 ⁇ m to About 1 mm, about 700 ⁇ m to about 1 mm, about 900 ⁇ m to about 1 mm, about 1 nm to about 900 ⁇ m, about 10 nm to about 900 ⁇ m, about 100 nm to about 900 ⁇ m, about 300 nm to about 900 ⁇ m, about 500
  • the porous tube is a front end cap coupled to the coupling nut portion formed on one side inside the front end of the porous tube, and the bolt portion formed on one side inside the rear end of the porous tube body Entering into and coupled to the coupling nut portion of the front closing cap, a plurality of gas through-holes are formed around the bolt portion around the bolt portion, may include a rear closure cap is formed along the outer peripheral surface curved groove
  • this may not be limited.
  • the front closure cap is coupled to the front end of the porous tube body so that the coupling nut portion is formed on one side of the front end of the porous tube body to be accommodated in the front end side of the porous tube body, and in order to maintain airtightness with the porous tube body
  • the front closing cap may be coupled in a state where the sealing ring is located, but may not be limited thereto.
  • the rear closure cap is formed with a bolt portion on one side, the rear end cap of the porous tube may be coupled to the rear end side of the porous tube to be engaged with the coupling nut portion of the front closing cap inside. However, this may not be limited.
  • the rear closure cap is also coupled between the porous tube body and the rear closure cap in a state in which a sealing ring is positioned to maintain airtightness.
  • the rear finishing cap has a plurality of gas through-holes are formed around the bolts around the bolts, and the gas introduced through the gas through-holes can be discharged into the micro bubbles through the pores of the porous pipes through the inside of the porous pipes.
  • the gas consists of hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof.
  • Vibration transmission member is attached to the outside of the rear closing cap of the porous tube may include transmitting the ultrasonic vibration generated in the body portion to the porous tube, but may not be limited thereto.
  • the vibration transmission member may be a vibration pin which is vibrated by the ultrasonic wave toward the ultrasonic vibrator that is outward, so that the ultrasonic vibration is transmitted to the porous tube by the vibration pin, but may not be limited thereto.
  • the body portion is formed in a tubular shape
  • the curved coupling groove is formed along the inner circumferential surface in the front
  • the front tube formed with the curved coupling groove is fixed to the rear end of the porous tube body
  • the body It is seated so as to flow between the curved coupling groove of the tube and the curved recess of the rear end cap
  • the elastic fixing ring is fixed to the porous tube body
  • the rear end of the main body screw is screwed
  • the center is equipped with an ultrasonic vibrator It may include, but is not limited to, a connection tube having a gas inlet hole connected to the gas supply line around the ultrasonic vibrator.
  • the main body tube is formed in a tubular shape having a predetermined diameter, and a curved coupling groove is formed on the inner circumferential surface of the front end so that the curved groove of the rear closing cap and the curved coupling groove of the main tube when the rear end of the porous tube body is connected to the front end of the body portion May be substituted for each other, but may not be limited thereto.
  • an elastic fixing ring is provided between the curved recessed groove of the rear closing cap facing each other and the curved coupling groove of the main body tube, and may include fixing the porous tube body to be flowable to the body portion. However, this may not be limited.
  • the elastic fixing ring may be to fix the porous tube body and the body portion, as well as to maintain the airtight between the porous tube body and the body portion.
  • the rear end of the main body tube is screw-coupled, the center of the connecting tube may be provided with an ultrasonic vibrator, but may not be limited thereto.
  • the ultrasonic vibrator is the same as a conventional ultrasonic vibrator, and the detailed description may be omitted and may be selectively driven by external control, but may not be limited thereto.
  • the frequency of the ultrasonic vibrator may be about 1 Hz to about 300 MHz, but may not be limited thereto.
  • the frequency of the ultrasonic vibrator is about 1 Hz to about 300 MHz, about 10 Hz to about 300 MHz, about 100 Hz to about 300 MHz, about 300 Hz to about 300 MHz, about 500 Hz to about 300 MHz, About 700 Hz to about 300 MHz, about 900 Hz to about 300 MHz, about 1 Hz to about 300 MHz, about 10 Hz to about 300 MHz, about 100 Hz to about 300 MHz, about 300 Hz to about 300 MHz, about 500 KHz to about 300 MHz, about 700 Hz to about 300 MHz, about 900 Hz to about 300 MHz, about 1 MHz to about 300 MHz, about 10 MHz to about 300 MHz, about 100 MHz to about 300 MHz, about 1 Hz to About 100 MHz, about 10 Hz to about 100 MHz, about 100 Hz to about 100 MHz, about 300 Hz to about 100 MHz, about 500 Hz to about 100 MHz, about 700 Hz to about 100 MHz, about 900 Hz to about 100 MHz, about
  • the average diameter of the micro bubbles generated in the porous tube may be about 1 nm to about 1,000 ⁇ m, but may not be limited thereto.
  • the average diameter of the microbubbles is about 1 nm to about 1,000 ⁇ m, about 10 nm to about 1,000 ⁇ m, about 100 nm to about 1,000 ⁇ m, about 300 nm to about 1,000 ⁇ m, about 500 nm to about 1,000 ⁇ m , About 700 nm to about 1,000 ⁇ m, about 900 nm to about 1,000 ⁇ m, about 1 ⁇ m to about 1,000 ⁇ m, about 10 ⁇ m to about 1,000 ⁇ m, about 100 ⁇ m to about 1,000 ⁇ m, about 300 ⁇ m to about 1,000 ⁇ m, about 500 ⁇ m to about 1,000 ⁇ m, about 700 ⁇ m to about 1,000 ⁇ m, about 900 ⁇ m to about 1,000 ⁇ m, about 1 nm to about 900 ⁇ m, about 10 nm to about 900 ⁇ m, about 1 nm to about 900
  • a cell culture medium comprising serum and antibiotic, wherein the cell culture medium contains microbubble water, and about 10 3 to about 10 18 about 1 mL of about 1 mL of the microbubble water. It provides a cell culture medium, comprising a microbubble having an average diameter of nm to about 1,000 ⁇ m.
  • the microbubble water may be prepared by a method such as pressure dissolution, rotary liquid flow type, static mixer type, ezekuta type, venturi type, pore type, rotary type, ultrasonic type, steam condensation type, and electrolysis type.
  • the microbubbles may include microbubbles having an average diameter of about 1 nm to about 1,000 ⁇ m, and may include about 10 3 to about 10 18 microbubbles per 1 mL of the microbubbles, but is not limited thereto. It may not be.
  • the average diameter of the microbubbles included in the number of microbubbles about 1 nm to about 1,000 ⁇ m, about 10 nm to about 1,000 ⁇ m, about 100 nm to about 1,000 ⁇ m, about 300 nm to about 1,000 ⁇ m, About 500 nm to about 1,000 ⁇ m, about 700 nm to about 1,000 ⁇ m, about 900 nm to about 1,000 ⁇ m, about 1 ⁇ m to about 1,000 ⁇ m, about 10 ⁇ m to about 1,000 ⁇ m, about 100 ⁇ m to about 1,000 ⁇ m, about 300 ⁇ m to about 1,000 ⁇ m, about 500 ⁇ m to about 1,000 ⁇ m, about 700 ⁇ m to about 1,000 ⁇ m, about 900 ⁇ m to about 1,000 ⁇ m, about 1 nm to about 900 ⁇ m, about 10 nm to about 900 ⁇ m, about 100 nm to About 900 ⁇ m, about 300 nm to about 900 ⁇ m, about 500 nm to about 900 ⁇ m, about
  • the microbubbles are, for example, about 10 3 to about 10 18 , about 10 4 to about 10 18 , about 10 5 to about 10 18 , about 10 6 per 1 mL of the microbubbles To about 10 18 , about 10 7 to about 10 18 , about 10 8 to about 10 18 , about 10 9 to about 10 18 , about 10 10 to about 10 18 , about 10 11 To about 10 18 , about 10 12 to about 10 18 , about 10 13 to about 10 18 , about 10 14 to about 10 18 , about 10 15 to about 10 18 , about 10 16 To about 10 18 , about 10 17 to about 10 18 , about 10 3 to about 10 17 , about 10 4 to about 10 17 , about 10 5 to about 10 17 , about 10 6 To about 10 17 , about 10 7 to about 10 17 , about 10 8 to about 10 17 , about 10 9 to about 10 17 , about 10 10 to about 10 17 , about 10 11 To about 10 17 , about 10 12 to about 10 17 , about 10 13 to about 10 17
  • the microbubbles may be present in water for a long time by stabilizing the microbubbles due to the self-pressing effect of the microbubbles.
  • the buoyancy effect of the bubbles may shorten the time that the microbubbles stay in the water, thereby causing a problem that the dissolved amount in the water is inhibited.
  • the number of the microbubbles per 1 mL of the microbubble water is less than about 10 3 outside the range, due to the number of individuals disappeared by the self-pressure effect of the microbubbles, solubility in water, sterilization of microbubbles, Problems such as surface activity and uniformity of the uniform number of fine bubbles in the bubble may be caused.
  • the microbubble water may be included in about 1 part by volume to about 50 parts by volume, preferably about 1 part by volume to about 20 parts by volume, based on 100 parts by volume of the cell culture medium. This may not be limited. For example, if the microbubble number is less than about 1 part by volume, a decrease in the number of microbubbles required for cell growth may be caused, whereas when the microbubble number exceeds about 50 parts by volume, Problems such as decreased cell growth may be caused due to reduced nutrients in the cell culture medium.
  • the microbubble water may apply various gases according to its function, for example, the gas may be hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, It may include, but is not limited to, a gas selected from the group consisting of ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof.
  • the microbubble water may include the gas, but may not be limited thereto.
  • the cells to which the culture medium can be applied include cancer cells selected from the group consisting of lung cancer cells, prostate cancer cells, gastric cancer cells, breast cancer cells, pancreatic cancer cells, colon cancer cells, and combinations thereof, osteoblasts Cells, kidney cells, fibroblasts, chondrocytes, hepatocytes, nerve cells, muscle cells, stem cells, and combinations thereof may be included, but may not be limited thereto.
  • the serum may include one selected from the group consisting of fetal bovine serum (FBS), fetal bovine serum (FCS), and combinations thereof, but may not be limited thereto.
  • FBS fetal bovine serum
  • FCS fetal bovine serum
  • a fourth aspect of the present disclosure provides a method of culturing cells comprising culturing the cells confluent in the culture medium; And replacing the culture medium of the cells with a culture medium containing microbubble water, wherein the average of about 10 3 to about 10 18 about 1 nm to about 1,000 ⁇ m per about 1 mL of the microbubble water. It provides a cell culture method comprising a microbubble having a diameter.
  • the microbubble water is contained in the cell culture medium at about 1 vol. To about 50 vol.
  • the microbubble water may be used to be sterilized under UV before being added to the cell culture medium, but may not be limited thereto.
  • the cells are lung cancer cells, prostate cancer cells, gastric cancer cells, breast cancer cells, pancreatic cancer cells, colon cancer cells, cancer cells selected from the group consisting of combinations thereof, osteoblasts, kidney cells, fibroblasts, It may include, but is not limited to, one selected from the group consisting of chondrocytes, hepatocytes, neurons, muscle cells, stem cells, and combinations thereof.
  • the serum may include one selected from the group consisting of fetal bovine serum (FBS), fetal bovine serum (FCS), and combinations thereof, but may not be limited thereto.
  • FBS fetal bovine serum
  • FCS fetal bovine serum
  • each of lung cancer cells A549, A549D9K, osteoblast MC3T3, fibroblast NIH3T3, and renal cell HEK293 using microbubble-containing cell culture medium.
  • the cell growth was promoted as compared to the control cultured cells using a medium containing no microbubbles.
  • FIG. 16 is photographed using a high-sensitivity charge coupled device (CCD) camera and an X20 microscope objective lens constituting a nano sight LM10-HS having a microbubble formed in the fuel and an LM14 having a 405 nm laser.
  • CCD charge coupled device
  • the fuel may include one selected from the group consisting of fossil fuel, biofuel, and combinations thereof, but may not be limited thereto.
  • the fossil fuel may include, but is not limited to, one selected from the group consisting of gasoline, diesel, lubricating oil, bunker oil, and combinations thereof.
  • the biofuel may include one selected from the group consisting of bioethanol, biomethanol, biodiesel, and combinations thereof, but may not be limited thereto.
  • the gasoline refers to petroleum oil in a volatile liquid state, which has a high calorific value, good fluidity, fast burning speed, high self-ignition temperature, and low generation of harmful compounds after combustion.
  • the diesel may have a high cetane number and thus have excellent ignition, no impurities, and a large calorific value, but may not be limited thereto.
  • the cetane number is a quantitative value indicating the ignition of the diesel fuel. As the cetane number is higher, it may be more difficult to cause a diesel knock phenomenon, but may not be limited thereto.
  • the microbubbles are filled with a gas filled with an extremely small cavity formed in a liquid. When the microbubbles are used as fuels, the generation of gases exhibiting a greenhouse effect such as carbon monoxide, carbon dioxide, and nitrogen compounds is significantly reduced. In addition, it can be easily transported in the form of gas or liquid, and bulk storage may also be possible.
  • the gas consists of hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof. It may include a gas selected from the group, but may not be limited thereto.
  • the hydrogen in the case of using hydrogen as the gas, the hydrogen may have a number of advantages in terms of efficiency because it can be made of water as a raw material, and can be recycled back to water after use, but may not be limited thereto. .
  • the concentration and diameter of microbubbles formed in the fuel show the concentration and diameter of microbubbles formed in the fuel.
  • the concentration of the microbubbles may increase in number over time through the self fragmentation of the formed microbubbles.
  • the microbubbles may be about 10 3 to about 10 18 microbubbles per 1 mL of the fuel, but may not be limited thereto.
  • the microbubbles are about 10 3 to about 10 18 , about 10 4 to about 10 18 , about 10 5 to about 10 18 , about 10 6 to about 10 mL of the fuel 10 18 , about 10 7 to about 10 18 , about 10 8 to about 10 18 , about 10 9 to about 10 18 , about 10 10 to about 10 18 , about 10 11 to about 10 18 , about 10 12 to about 10 18 , about 10 13 to about 10 18 , about 10 14 to about 10 18 , about 10 15 to about 10 18 , about 10 16 to about 10 18 , about 10 17 to about 10 18 , about 10 3 to about 10 17 , about 10 4 to about 10 17 , about 10 5 to about 10 17 , about 10 6 to about 10 17 , about 10 7 to about 10 17 , about 10 8 to about 10 17 , about 10 9 to about 10 17 , about 10 10 to about 10 17 , about 10 11 to about 10 17 , about 10 12 to about 10 17 , about 10 13 to about 10 17 , about 10 14 To about
  • the diameter of the microbubbles may not be uniformly distributed immediately after their formation, but may include microbubbles having a uniform diameter over time, but may not be limited thereto. have.
  • the microbubbles may have a diameter of about 1 nm to about 1,000 ⁇ m, but may not be limited thereto.
  • the diameter of the microbubbles may be about 1 nm to about 1,000 ⁇ m, about 10 nm to about 1,000 ⁇ m, about 100 nm to about 1,000 ⁇ m, about 300 nm to about 1,000 ⁇ m, about 500 nm to about 1,000 ⁇ m, About 700 nm to about 1,000 ⁇ m, about 900 nm to about 1,000 ⁇ m, about 1 ⁇ m to about 1,000 ⁇ m, about 10 ⁇ m to about 1,000 ⁇ m, about 100 ⁇ m to about 1,000 ⁇ m, about 300 ⁇ m to about 1,000 ⁇ m, about 500 ⁇ m to about 1,000 ⁇ m, about 700 ⁇ m to about 1,000 ⁇ m, about 900 ⁇ m to about 1,000 ⁇ m, about 1 nm to about 900 ⁇ m, about 10 nm to about 900 ⁇ m, about 100 nm to about 900 ⁇ m, about 300 nm to About 900 ⁇ m, about 500 nm to about 900 ⁇ m, about 700 nm to about 700
  • FIG. 18a shows the viscosity of the fuel and shows the viscosity of the conventional gasoline and gasoline in which microbubbles are formed.
  • the viscosity of the gasoline in which the microbubble is formed may be about 0.4 MPa ⁇ s to about 0.7 MPa ⁇ s, but may not be limited thereto.
  • the viscosity of the microbubble formed gasoline is about 0.4 MPa ⁇ s to about 0.7 MPa ⁇ s, about 0.45 MPa ⁇ s to about 0.7 MPa ⁇ s, about 0.5 MPa ⁇ s to about 0.7 MPa ⁇ s, about 0.55 MPa ⁇ s to about 0.7 MPa ⁇ s, about 0.6 MPa ⁇ s to about 0.7 MPa ⁇ s, about 0.65 MPa ⁇ s to about 0.7 MPa ⁇ s, about 0.4 MPa ⁇ s to about 0.65 MPa ⁇ s, about 0.45 MPa S to about 0.65 MPa ⁇ s, about 0.5 MPa ⁇ s to about 0.65 MPa ⁇ s, about 0.55 MPa ⁇ s to about 0.65 MPa ⁇ s, about 0.6 MPa ⁇ s to about 0.65 MPa ⁇ s, about 0.4 MPa ⁇ s To about 0.6 MPa ⁇ s, about 0. 0.45
  • the viscosity of the fuel is an internal resistance that appears when the fuel flows. If the viscosity of the fuel is high, the injection characteristics are deteriorated and the injection pressure must be increased when the fuel is injected, and engine performance and combustion characteristics may be deteriorated, but the present invention is not limited thereto. have.
  • FIG. 18B shows the surface tension of the fuel, and shows the surface tension of the gasoline and the gasoline in which the fine bubble is formed.
  • the surface tension of the microbubble formed gasoline may be about 12 dyn / cm to about 15 dyn / cm, but may not be limited thereto.
  • the surface tension of the microbubble formed gasoline is about 12 dyn / cm to about 15 dyn / cm, about 12.5 dyn / cm to about 15 dyn / cm, about 13 dyn / cm to about 15 dyn / cm, About 13.5 dyn / cm to about 15 dyn / cm, about 14 dyn / cm to about 15 dyn / cm, about 14.5 dyn / cm to about 15 dyn / cm, about 12 dyn / cm to about 14.5 dyn / cm, about 12.5 dyn / cm to about 14.5 dyn / cm, about 13 dyn / cm to about 14.5 dyn / cm, about 13.5 dyn / cm to about 14.5 dyn / cm, about 14 dyn / cm to about 14.5 dyn / cm, about 12 dyn / cm to about
  • a sixth aspect of the present disclosure includes a liquid tank into which a liquid is injected; A gas supply line unit supplying gas to the liquid tank; And it provides a high-efficiency mixed fuel manufacturing apparatus comprising a porous tube installed inside the liquid tank.
  • a liquid tank 510 into which a liquid is injected is prepared.
  • the liquid tank 510 may be a form in which a predetermined liquid is injected and the injected liquid does not leak, like the normal liquid tank, but may not be limited thereto.
  • the liquid tank 510 may include, but is not limited to, an upper portion of the liquid tank 510 sealed by a sealing lid.
  • the airtight lid includes a gas supply line part connected to the airtight lid, a pressure gauge provided in the airtight lid to measure the pressure in the liquid tank 510, and a pressure control valve to control the pressure inside the liquid tank 510. It may be included, but may not be limited thereto.
  • the liquid may include, but is not limited to, one selected from the group consisting of water, high viscosity materials, and combinations thereof.
  • the high viscosity material may be selected from the group consisting of polymer, fuel, and combinations thereof, but may not be limited thereto.
  • the high viscosity material may include one selected from the group consisting of polymers, fossil fuels, biofuels, and combinations thereof, for example, lubricating oil, gasoline, diesel, lubricating oil, bunker oil, bio ethanol, Bio methanol, bio diesel, and combinations thereof may be selected from the group consisting of, but may not be limited thereto.
  • the gas supply line part includes an inlet valve 525 into which gas is injected, a pressure gauge measuring a pressure of the injected gas, and a supply pipe 520 through which the injected gas moves.
  • the gas consists of hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof. It may include a gas selected from the group, but may not be limited thereto.
  • the gas supply line part may further include a gas tank connected to the inlet valve 525, but may not be limited thereto.
  • the gas contained in the gas tank may be injected into the liquid tank 510 through the supply pipe 520, and injected through the supply pipe 520.
  • the gas may be supplied to the porous tube 530, but may not be limited thereto.
  • the gas stored in the gas tank when included in the liquid, the gas generates water after combustion, does not generate pollutants, and can be used efficiently because of its large combustion rate. It may not be limited.
  • the porous tube 530 may have a hole of about 1 nm to about 1 mm, but may not be limited thereto.
  • the porous tube has about 1 nm to about 1 mm, about 10 nm to about 1 mm, about 100 nm to about 1 mm, about 300 nm to about 1 mm, about 500 nm to about 1 mm, about 700 Nm to about 1 mm, about 900 nm to about 1 mm, about 1 ⁇ m to about 1 mm, about 10 ⁇ m to about 1 mm, about 100 ⁇ m to about 1 mm, about 300 ⁇ m to about 1 mm, about 500 ⁇ m to About 1 mm, about 700 ⁇ m to about 1 mm, about 900 ⁇ m to about 1 mm, about 1 nm to about 900 ⁇ m, about 10 nm to about 900 ⁇ m, about 100 nm to about 900 ⁇ m, about 300 nm to about 900 ⁇ m,
  • the porous tube may be converted into microbubbles while passing through the porous tube, but may not be limited thereto.
  • the gas when the gas is formed as a micro bubble while passing through the hole of the porous tube, the micro bubble may be converted into nanobubbles due to the self-shrink effect of the micro bubble, but may not be limited thereto. .
  • nanobubbles were first prepared.
  • Hydrogen nanobubble water was prepared by a pressure dissolving method by using a microbubble water producing apparatus using an ultrasonic vibrator according to FIG. 1 without applying ultrasonic waves, and injecting gas using a microporous filter.
  • Oxygen nanobubble water was prepared by a pressure dissolving method by using a microbubble water producing apparatus using an ultrasonic vibrator according to FIG.
  • the water quality test of the prepared oxygen nanobubble water was carried out by a nanoparticle tracking analysis (NTA, LM10-HSBFT14, UK) method. As shown in FIGS. 5A and 5B, the nanobubble included in the oxygen nanobubble water The average diameter of the oxygen bubbles was about 87 nm and the concentration was about 2.62 ⁇ 10 17 per about mL.
  • lung cancer cell A549 (purchased from ATCC) contains 10% FetalClone III (Lonza) and 1% penicillin-streptomycin (MP) in DMEM (Cellgro), and A549D9K (D9K mutated CXCR2 expressed in A549) is DMEM (Cellgro).
  • An optical measurement system including a phase contrast microscope (Nikon TiU), camera (Quantiem: 512SC) and NIS-element software (Nikon Instruments Inc.). After replacing with a medium containing nanobubble water, images (15 ⁇ ) of each cell were analyzed in each well every given time.
  • cell growth was significantly increased compared to the control group.
  • nanohydrogen gasoline was manufactured.
  • hydrogen gas having a purity of 99.995% Shinyoung Special Gas
  • gasoline having a octane number of 91 to 94 Hydrophilic Materials
  • a microbubble manufacturing apparatus having the same structure as the manufacturing apparatus according to an embodiment of the present application (Fig. 19).
  • the microbubble manufacturing apparatus includes a liquid tank 510 and a porous tube 530 into which gasoline is injected, and the hydrogen gas flowing out of the hydrogen gas tank through the hydrogen gas supply line to the porous tube 530. Injected.
  • the porous tube 530 includes a porous material and is installed below the liquid tank 510 to be immersed in the gasoline.
  • the hydrogen gas injected into the porous tube 530 formed nano hydrogen bubbles on the surface of the porous tube 530.
  • the nanohydrogen bubble formed on the surface of the porous tube 530 has a holding force for holding the nanohydrogen bubble on the solid surface and a detaching force for separating the nanohydrogen bubble from the solid surface. As shown in FIG. 20, as the nanohydrogen bubble grows, the separation force becomes larger than the holding force, and thus the nanohydrogen bubble may be separated from the surface of the porous tube 530.
  • Example 4 the production of gasoline formed with nano hydrogen bubbles at room temperature and atmospheric pressure was performed, and the amount of evaporated gas was charged due to the high volatility of gasoline during the production of the nano hydrogen bubbles. Finally, after stopping the microbubble manufacturing apparatus, a gasoline in which the hydrogen hydrogen bubbles were formed in the liquid tank 510 was obtained through an outlet valve 540, and the obtained gasoline in which the hydrogen hydrogen bubbles were formed was normal at room temperature and atmospheric pressure. Stored in a plastic bottle.
  • the experimental engine used in the experiment was a Hyundai EF Sonata using an electronically controlled engine with a displacement of about 2,000 cc in series four-cylinder.
  • Example 4 As shown in Table 1, the gasoline used in Example 4 is to form a nano-hydrogen bubble in the gasoline (modern oil bank) and the gasoline is commercially available in Korea. Through the measurement of the calorific value of the two gasoline was to determine the effect of the nano-hydrogen bubble on the calorific value of the gasoline, through the results of this experiment it was confirmed that there is no significant difference in the calorific value even if the nano-hydrogen bubble in the existing gasoline.
  • Table 2 shows the viscosity of the existing gasoline and gasoline formed nano-hydrogen bubble. As shown in Table 2, the average viscosity of conventional gasoline is about 0.58 MPa ⁇ s, and the average viscosity of gasoline that forms nanohydrogen bubbles is about 0.55 MPa ⁇ s, and the viscosity of gasoline that forms nanohydrogen bubbles is about 0.03. It was found that MPa ⁇ s was lower. An LVT viscometer (Brookfield Engineering Laboratories INC., USA) was used to measure the viscosity of the gasoline.
  • the viscometer used in the measurement includes a body with an indicator needle, a spindle, a pedestal, and a circular level, from about 1.0 MPa ⁇ s to about 2.0 at a spindle speed of about 0.3 rpm to about 60 rpm.
  • the viscosity up to ⁇ 10 6 MPa ⁇ s can be measured.
  • the result was obtained after the guide stabilized at the set spindle speed.
  • the kinematic viscosity was calculated using the conversion factor when the value indicated by the guide was determined.
  • Viscosity of the gasoline is an internal resistance that appears when the gasoline flows, when the viscosity of the gasoline is high, the injection characteristics deteriorate to increase the injection pressure during fuel injection, engine performance and combustion characteristics may be deteriorated.
  • Table 3 shows the surface tension of the conventional gasoline and gasoline formed with nano hydrogen bubbles.
  • the surface tension of the gasoline was measured using a Du Nouy tension meter (Du Nouy tension meter, No.3179, Itoh Seisakusho, Japan) using the Du Nouy ring method for measuring the surface tension of the liquid. .
  • the average surface tension of the existing gasoline is about 13.54 dyn / cm
  • the average surface tension of the gasoline forming the nano-hydrogen bubble is about 13.93 dyn / cm
  • the surface tension of the gasoline forming the nano-hydrogen bubble It was found that about 0.39 dyn / cm higher.
  • the average value of the nano-hydrogen bubbles and the number of population changes over time according to the time of the nano-hydrogen gasoline is formed.
  • a nano particle tracking analysis (NTA) device was used, and the device irradiated a laser to record an image of a nano hydrogen bubble moving by Brownian motion in a liquid.
  • the laser irradiation device is mounted under a microscope lens, and nanohydrogen bubbles in the liquid specimen that pass through the laser beam path are represented by small white dots that move or vibrate by the device.
  • NTA 2.3 analytic software tracked each nanohydrogen bubble and measured the diffusion coefficient (D t ) of the nanohydrogen bubble.
  • D t diffusion coefficient
  • K B is Boltzmann's constant
  • T is temperature
  • ⁇ is the viscosity of the liquid.
  • 21A to 21C are diagrams illustrating power characteristics of a conventional gasoline and a gasoline in which nano hydrogen bubbles according to the fourth embodiment are formed.
  • 21A to 21C illustrate changes in torque values, fuel consumption rates, and power values measured while increasing the amount of change in the accelerator while the engine rotation speed is fixed at about 3,000 rpm.
  • FIG. 21B illustrates a brake specific fuel consumption according to the engine load according to the present embodiment
  • FIG. 21C illustrates the engine according to the present embodiment. It shows the power value according to the load.
  • the torque (output) value is a representative value for evaluating output characteristics. In the case of gasoline with a hydrogen hydrogen bubble, the torque value was increased in a low load and a heavy load region compared to a conventional gasoline.
  • 22A to 22D show the harmful exhaust emission characteristics according to the engine load of the conventional gasoline and the gasoline in which the nanohydrogen bubble is formed according to the fourth embodiment, and shows the generation amount of carbon monoxide, carbon dioxide, hydrocarbons, and nitrogen compounds.
  • the harmful exhaust emissions are known as respiratory diseases and major greenhouse gases, and these harmful exhaust emissions are closely related to combustion performance.
  • carbon monoxide and carbon dioxide of FIGS. 22A and 22B it was found that combustion of gasoline in which nano hydrogen bubbles are formed is lower than that of general gasoline in the overall engine load region.
  • gasoline and nano-hydrogen gasoline formed gasoline in all areas it was found that the amount of hydrocarbon generated is significantly lower than conventional gasoline combustion.
  • the principle of hydrocarbon generation is that carbon and hydrogen, which are the main components of fuel, are not emitted during the combustion process, and as shown in FIG. 22C, in the case of gasoline formed with nanohydrogen bubbles, combustion of hydrocarbons is more complete than that of gasoline. It was also thought to be reduced.
  • nitrogen oxide as shown in FIG. 22d, it can be seen that the amount of the compound decreases in the overall engine load region.
  • the nitrogen oxides are directly related to the combustion temperature, and as the combustion pressure increases, the combustion temperature increases, thereby increasing the nitrogen oxides. That is, the amount of nitrogen oxide is also increased as the flame temperature increases.
  • high flame temperature caused a high flame temperature in the combustion chamber, which was thought to increase nitrogen oxides.
  • the engine used in this experiment is a state in which the exhaust aftertreatment is removed, and in general, since the nitrogen oxides can be easily reduced to the three-way catalytic aftertreatment purification device, the use of the aftertreatment device or the ignition timing or air-fuel ratio (fuel injection amount) It would be possible to reduce it sufficiently if the
  • gas supply line portion 310 gas cylinder 320: supply pipe
  • porous tube 420 body portion 421: body tube
  • gas inlet hole 426 ultrasonic vibrator 430: vibration transmission member
  • bolt portion 452 gas through hole 453: curved groove 510: liquid tank

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  • Oil, Petroleum & Natural Gas (AREA)
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Abstract

La présente invention concerne un dispositif pour produire de l'eau à microbulles à l'aide d'un vibreur à ultrasons pouvant produire en masse de l'eau à bulles présentant une quantité optimisée de bulles dissoutes, un élément d'évacuation de microbulles, un milieu de culture de cellules contenant l'eau à microbulles, un procédé de culture de cellules l'utilisant, un carburant mélangé à haute efficacité utilisant des microbulles et un procédé de fabrication associé.
PCT/KR2013/010476 2012-12-04 2013-11-18 Dispositif de production d'eau à microbulles utilisant un vibreur à ultrasons, un milieu de culture de cellules contenant de l'eau à microbulles, un procédé de culture de cellules l'utilisant, un carburant mélangé à haute efficacité utilisant des microbulles et procédé de fabrication associé Ceased WO2014088242A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201380072289.1A CN104968607A (zh) 2012-12-04 2013-11-18 使用超声振动器生产微气泡水的设备、包含微气泡水的细胞培养基、使用该细胞培养基的细胞培养方法、使用微气泡的高效混合燃料以及用于制造高效混合燃料的设备
US14/649,401 US9908089B2 (en) 2012-12-04 2013-11-18 Device for producing microbubble water by using ultrasonic vibrator, cell culture medium containing microbubble water, cell culturing method using same, high efficiency mixed fuel using microbubbles, and method for manufacturing same

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
KR20120139663 2012-12-04
KR10-2012-0139663 2012-12-04
KR1020130038064A KR101505917B1 (ko) 2012-06-05 2013-04-08 초음파진동자를 이용한 미세버블수 제조 장치
KR10-2013-0038064 2013-04-08
KR10-2013-0047000 2013-04-26
KR20130047000 2013-04-26
KR1020130140180A KR101455115B1 (ko) 2013-04-26 2013-11-18 미세버블을 이용한 고효율 혼합 연료 및 그의 제조장치
KR10-2013-0140181 2013-11-18
KR1020130140181A KR101588246B1 (ko) 2012-12-04 2013-11-18 미세버블수를 함유한 세포 배양 배지 및 이를 이용한 세포 배양 방법
KR10-2013-0140180 2013-11-18

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CN105032261A (zh) * 2015-07-20 2015-11-11 中国石油天然气股份有限公司 一种制备纳米分散体系的方法及装置
CN105148819A (zh) * 2015-07-20 2015-12-16 中国石油天然气股份有限公司 一种制备微米级气泡分散体系的超声波振荡方法及装置
CN105148760A (zh) * 2015-07-20 2015-12-16 中国石油天然气股份有限公司 一种制备微米级气泡分散体系的孔板喷射方法及装置
CN117401799A (zh) * 2023-10-07 2024-01-16 中规(北京)认证有限公司 富氢水制备装置及方法

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JP2008019359A (ja) * 2006-07-13 2008-01-31 Shinkawa Yoshiro エマルジョン組成物の製造方法並びにエマルジョン化装置
KR20120084134A (ko) * 2011-01-19 2012-07-27 나현호 마이크로 버블 헤드 및 이를 구비하는 마이크로 버블 생성장치

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JP2008019359A (ja) * 2006-07-13 2008-01-31 Shinkawa Yoshiro エマルジョン組成物の製造方法並びにエマルジョン化装置
KR20120084134A (ko) * 2011-01-19 2012-07-27 나현호 마이크로 버블 헤드 및 이를 구비하는 마이크로 버블 생성장치

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105032261A (zh) * 2015-07-20 2015-11-11 中国石油天然气股份有限公司 一种制备纳米分散体系的方法及装置
CN105148819A (zh) * 2015-07-20 2015-12-16 中国石油天然气股份有限公司 一种制备微米级气泡分散体系的超声波振荡方法及装置
CN105148760A (zh) * 2015-07-20 2015-12-16 中国石油天然气股份有限公司 一种制备微米级气泡分散体系的孔板喷射方法及装置
CN117401799A (zh) * 2023-10-07 2024-01-16 中规(北京)认证有限公司 富氢水制备装置及方法

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