WO2014088242A1 - Device for producing microbubble water by using ultrasonic vibrator, cell culture medium containing microbubble water, cell culturing method using same, high efficiency mixed fuel using microbubbles, and method for manufacturing same - Google Patents

Abstract

The present application provides a device for producing microbubble water using an ultrasonic vibrator capable of mass producing bubble water having the maximized amount of dissolved bubbles, a microbubble discharge member, a cell culture medium containing the microbubble water, a cell culturing method using same, a high efficiency mixed fuel using microbubbles, and a method for manufacturing same.

Description

Apparatus for producing microbubble water using an ultrasonic vibrator, cell culture medium containing microbubble water and cell culture method using the same, and high efficiency mixed fuel using microbubble and apparatus for producing same

The present application is a microbubble water production apparatus using an ultrasonic vibrator capable of mass production of the bubble water maximized dissolved gas, microbubble release member, cell culture medium containing microbubble water satisfying both economical and safety and cell culture method using the same And a high efficiency mixed fuel using fine bubbles and a manufacturing apparatus thereof.

Recently, water having a higher dissolved gas amount than general water has been produced. For example, it is used in the process of purifying wastewater from barns, etc., and oxygen (gas) water with high dissolved oxygen has been mainly used for supplying oxygen (gas) to fish in aquaculture farms. When the oxygen water is consumed by animals or humans, it is quickly absorbed in the body, the metabolism becomes active, and has many effects such as being strong against various diseases and pests. It is often applied. In addition, in the case of plants, the soil environment is improved, and oxygen is directly supplied to leaves or roots to grow more robustly, resulting in stronger pests, resulting in increased production. For this reason, various methods and devices have been provided for producing oxygen (gas, bubble) water. Conventionally provided gas water production method and manufacturing apparatus used a method of increasing the dissolved gas amount while mixing the water at a high speed by using a motor, the method is difficult to obtain the desired dissolved gas amount in a short time as well as fine bubbles It is difficult to produce. In addition, there was a method of producing oxygen (gas) water by electrolysis or refrigeration, but in this case, the equipment is very expensive and the mixed oxygen (gas) is present in the water in a bubble state, and the dissolved oxygen amount rapidly with time. Along with this disadvantage, there is a problem in that the size of the bubbles cannot be adjusted. In addition, the method of preparing oxygen (gas) water by supplying fine air bubbles has the advantage of producing a high concentration of oxygen (gas) water, but in order to supply the nanobubble to continuously supply the nanobubble under high pressure conditions It must be produced and supplied, and this also has a disadvantage that a large amount of oxygen (gas) is present in the bubble state in the water, and the dissolved oxygen amount drops quickly over time.

Serum is a complex product mixed with various substances and is used as an additive in a basic culture medium in a cell culture chamber, and contains growth factors, hormones, and components that stimulate cells in cell culture. In general, fetal bovine serum (FBS) is the most widely used. The fetal bovine serum is serum isolated from bovine blood during pregnancy, especially in animal cell culture, which is a fundamental step in biotechnology-related experiments, as well as vaccines, protein medicines and treatments that have become increasingly fast worldwide in recent years. It is a raw material used for development, such as an antibody. However, in recent years, the supply of fetal bovine serum is limited due to the mad cow disease fluctuations, the price has soared, there is a problem that can be infected with mad cow disease, there is a problem that the safety of the target product is not sufficiently secured. The domestic market of fetal bovine serum is about 20 billion won, and the global market size is about 2 trillion won. Of these, about 85% are made in the United States, and 15% are made in Australia and New Zealand. There is no domestic production and sales, and the situation that can be dealt with if the ban is imposed is very low at present. Thus, there is a great need for the development of other serum or culture medium components that can replace fetal calf serum. Korean Patent No. 10-0394430 discloses a method for culturing a human cell comprising human serum in a medium used for culturing a human cell. However, the patent can be infected with human viruses, such as AIDS, and the like, there is a limit to the supply, the effect is not great in other animal cell culture, the effect is significantly lower than the medium using fetal bovine serum, even in human cell culture, fetal bovine serum Cannot be replaced completely. However, the use of fetal bovine serum can be reduced somewhat, but there is a problem that the economic efficiency is also greatly reduced. Thus, there is a need to develop a serum that can replace fetal calf serum or other medium components that can promote cell growth while reducing the amount of fetal calf serum. Thus, the use of expensive fetal bovine serum was reduced by containing nanobubble water as a component in cell culture.

On the other hand, fossil fuel is mainly used as the underlying energy source on the earth, and since such fossil fuels have limitations depending on their reserves, they should be used efficiently. Middle East crude oil prices continue to rise year by year and are expected to reach around $ 243.8 / BL in 2035, according to the 2010 International Energy Agency (IEA) data. As of 2010, the average oil consumption per day is about 87.3 million BL. The problem of increasing oil consumption and rising oil prices is increasing, and the development of next generation fuels is a concern. Although research has been conducted to develop fuel cells or hydrogen fuels as alternative energy sources, it is necessary to develop highly efficient energy sources to suppress petroleum consumption. Korean Patent No. 10-1071461 relates to a microbubble generating device, and discloses a microbubble generating device for converting a mixed oil mixed with water and a fuel into an emulsion state. However, the above-described method has a problem that the emulsion state may become unstable and the separation of water and fuel may occur when a predetermined time has elapsed in the mixed oil converted into the emulsion state. In order to solve this problem, there is a demand for the development of a stable, high-efficiency fuel without separation.

An object of the present application is to receive the ultrasonic vibration propagated from the ultrasonic vibrator to vibrate the porous tube to release the gas into the micro-bubble, when generating the micro-bubble from the porous tube, it is easy to aggregate between the micro-bubbles and desorption of the micro-bubble and the porous tube In addition, since high-pressure gas injection is not required to generate micro bubbles, it is possible to mass-produce with a relatively simple facility, which can drastically reduce production costs, and allow the porous tube to emit relatively uniform micro bubbles. An object of the present invention is to provide a microbubble water manufacturing apparatus using an ultrasonic vibrator capable of producing a large amount of bubble water in a relatively short time.

Another object of the present invention is to provide a microbubble emitting member, which is applied to the porous tube by ultrasonic vibration generated in the ultrasonic vibrator to facilitate the desorption of the micro bubbles generated in the porous tube.

It is another object of the present invention to provide a cell culture medium containing microbubble water, which is economical and safe by reducing the use of expensive fetal bovine serum, and a cell culture method using the same.

Still another object of the present invention is to provide a highly efficient mixed fuel using a microbubble, and a manufacturing apparatus thereof.

However, the problem to be solved by the present application is not limited to the above-mentioned problem, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

A first aspect of the present disclosure is a liquid tank containing a liquid; A liquid circulation line unit for forcibly circulating a liquid contained in the liquid tank; A gas supply line unit supplying gas to the liquid tank; And installed inside the liquid tank in a state of being connected to the gas supply line part, and when the gas supplied from the gas supply line part is discharged into the micro bubbles, the micro bubbles are vibrated by ultrasonic waves so as not to aggregate with each other. Provided is a microbubble water production apparatus using an ultrasonic vibrator, comprising a plurality of microbubble emitting member to shake off the micro-bubbles.

The second side of the present application is provided to be connected to one side of the porous tube, the body portion is generated vibration by the ultrasonic vibrator provided therein; And a vibration transmitting member attached to one side of the porous tube to transfer the ultrasonic vibration generated from the body to the porous tube, wherein the ultrasonic vibration is applied to the porous tube through the vibration transmitting member. It provides a microbubble discharge member that is to desorb the micro-bubbles generated in the tube.

According to a third aspect of the present invention, there is provided a cell culture medium comprising serum and antibiotic, wherein the cell culture medium contains microbubble water, and about 10 ³ to about 10 ¹⁸ about 1 mL of about 1 mL of the microbubble water. It provides a cell culture medium, comprising a microbubble having an average diameter of nm to about 1,000 μm.

A fourth aspect of the present disclosure provides a method of culturing cells comprising culturing the cells confluent in the culture medium; And replacing the culture medium of the cells with a culture medium containing microbubble water, wherein the average of about 10 ³ to about 10 ¹⁸ about 1 nm to about 1,000 μm per about 1 mL of the microbubble water. It provides a cell culture method comprising a microbubble having a diameter.

A fifth aspect of the present application, fuel; And a fine bubble formed in the fuel.

A sixth aspect of the present disclosure includes a liquid tank into which a liquid is injected; A gas supply line unit supplying gas to the liquid tank; And it provides a high-efficiency mixed fuel manufacturing apparatus comprising a porous tube installed inside the liquid tank.

The apparatus for manufacturing microbubble water using the ultrasonic vibrator according to the present invention firstly vibrates by receiving ultrasonic vibration propagated from the ultrasonic vibrator through a porous tube in which gas of low pressure supplied from the outside is discharged into fine bubbles, and the microbubble is a porous tube. By adhering to the surface of, or desorbing from the porous tube before agglomerating with each other, it is possible to produce a bubble water maximized the amount of dissolved bubbles in the liquid contained in the liquid tank, relatively high pressure gas injection is not necessary to generate micro bubbles It is possible to mass-produce high quality bubble water with simple equipment, which can drastically reduce the production cost for producing high quality bubble water. Second, by remaining residual gas that is not mixed with the liquid in the liquid tank to maintain the atmosphere in the liquid tank gas saturation, it is possible to maximize the amount of dissolved gas of the bubble water, and to maintain a sufficient amount of dissolved gas over time Bubble water can be prepared. Third, very small and relatively uniform microbubbles are generated so that the microbubbles are efficiently dissolved in the liquid (water), a predetermined dissolved gas ratio of bubble water can be achieved in a relatively short time. Fourth, it is possible to generate a small and uniform microbubble in the high viscosity material. Fifth, it is possible to manufacture high-efficiency clean energy, including microbubbles.

In the microbubble emitting member according to the present application, ultrasonic vibration generated in an ultrasonic vibrator may be applied to a porous tube to facilitate desorption of microbubbles generated in the porous tube.

The cell culture medium according to the present invention can reduce the amount of expensive fetal bovine serum by including microbubble water, and exhibit the same or more cell growth promoting effect as the culture medium containing fetal bovine serum, and the microbubble water itself is safe. Because of this, there is an advantage that can be cultured cells economically and safely.

The high-efficiency mixed fuel and the high-efficiency mixed fuel manufacturing apparatus according to the present invention, by forming a microbubble in the existing fuel has the effect of combustion of fuel, fuel efficiency, further energy saving, and harmful emissions. Specifically, the microbubbles included in the fuel may improve the efficiency of the fuel by reducing the friction force generated between the inner surface of the pipeline and the fuel when the fuel passes through the pipeline, the amount of harmful emissions generated after combustion of the fuel Can be reduced to a significant level.

Figure 1 shows a microbubble water generating device according to an embodiment of the present application.

Figure 2 is a cross-sectional view showing a microbubble emitting member according to an embodiment of the present application.

Figure 3 is an exemplary view showing an operating state of the microbubble emitting member according to an embodiment of the present application.

4A and 4B are bubble size and concentration measurement test tables of hydrogen microbubble water according to an embodiment of the present application.

5a and 5b is a bubble size and concentration measurement test table of the oxygen microbubble water according to an embodiment of the present application.

FIG. 6 is an analysis of the growth of lung cancer cells A549 cultured at various times in a cell culture medium containing hydrogen microbubbles and oxygen microbubbles according to an embodiment of the present disclosure using optical image cell counting.

7 is a cell image of lung cancer cell A549 according to an embodiment of the present application.

FIG. 8 is an analysis of growth of lung cancer cells A549D9K cultured at various times in a cell culture medium containing hydrogen microbubbles and oxygen microbubbles according to an embodiment of the present disclosure using optical image cell counting.

9 is a cell image of lung cancer cell A549D9K according to an embodiment of the present application.

10 is analyzed using optical image cell counting the growth of osteoblast MC3T3 cultured at various times in a cell culture medium containing hydrogen microbubble number and oxygen microbubble number according to an embodiment of the present application.

Figure 11 analyzes the cell image of osteoblast MC3T3 according to an embodiment of the present application.

12 is analyzed using optical image cell counting the growth of fibroblast NIH3T3 cultured at various times in a cell culture medium containing hydrogen microbubble number and oxygen microbubble number according to an embodiment of the present application.

Figure 13 analyzes the cell image of the fibroblast NIH3T3 according to an embodiment of the present application.

14 is analyzed using optical image cell counting the growth of kidney cells HEK293 cultured at various times in a cell culture medium containing hydrogen microbubble number and oxygen microbubble number according to an embodiment of the present application.

15 is a cell image of kidney cells HEK293 according to an embodiment of the present application.

FIG. 16 is a high sensitivity charge coupled device (CCD) camera and an X20 including a nano sight LM10-HS and LM14 having a 405 nm laser after 121 days of microbubble formed gasoline according to an embodiment of the present disclosure. It is an image of microbubbles taken using a microscope objective lens.

Figure 17a shows the change in the number of microbubble population over time of the gasoline with a microbubble formed in accordance with an embodiment of the present application, Figures 17b to 17d is after the formation of each microbubble gasoline, after 76 days, And size distribution of the microbubbles after 121 days.

Figure 18a shows the viscosity of the existing gasoline and gasoline formed microbubble according to an embodiment of the present application, Figure 18b shows the surface tension of the existing gasoline and gasoline formed microbubble according to an embodiment of the present application. .

19 is a structural diagram showing an apparatus for manufacturing a high efficiency mixed fuel using a microbubble according to an embodiment of the present application.

20 is a schematic diagram showing the generation of microbubbles on the surface of the porous material according to an embodiment of the present application.

21A to 21C illustrate power characteristics of a conventional gasoline and a gasoline in which a microbubble is formed according to an embodiment of the present disclosure.

22a to 22d show the generation rate of harmful exhaust emissions according to the engine load of the existing gasoline and gasoline with a microbubble formed according to an embodiment of the present application.

DETAILED DESCRIPTION Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art may easily implement the present disclosure. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. In the drawings, parts irrelevant to the description are omitted for simplicity of explanation, and like reference numerals designate like parts throughout the specification.

Throughout this specification, when a portion is "connected" to another portion, this includes not only "directly connected" but also "electrically connected" with another element in between. do.

Throughout this specification, when a member is located “on” another member, this includes not only when one member is in contact with another member but also when another member exists between the two members.

Throughout this specification, when a part is said to "include" a certain component, it means that it can further include other components, without excluding the other components unless specifically stated otherwise. As used throughout this specification, the terms "about", "substantially" and the like are used at, or in the sense of, numerical values when a manufacturing and material tolerance inherent in the stated meanings is indicated, Accurate or absolute figures are used to assist in the prevention of unfair use by unscrupulous infringers. As used throughout this specification, the term "step to" or "step of" does not mean "step for."

Throughout this specification, the term “combination of these” included in the expression of the form of Markus means one or more mixtures or combinations selected from the group consisting of the elements described in the expression of the form of Markus, wherein the components It means to include one or more selected from the group consisting of.

Throughout this specification, the description of “A and / or B” means “A or B, or A and B”.

Throughout this specification, “microbubbles” shall include microbubbles of micrometer size and / or nanobubbles of nanometer size, and the size of the microbubbles may be those having an average diameter of about 1 nm to about 1,000 μm. However, this may not be limited. For example, the size of the microbubbles may have an average diameter of about 1 μm to about 1,000 μm, and the size of the nanobubbles may have an average diameter of about 1 nm to about 1,000 nm.

Throughout this specification, gases used to form microbubbles include hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and It may include a gas selected from the group consisting of combinations thereof, but may not be limited thereto. The nitrogen-containing compound gas is not particularly limited as long as it is in a gaseous state as a compound containing nitrogen. For example, the nitrogen-containing compound gas may include, but may not be limited to. The carbon-containing compound gas is not particularly limited as long as it is a compound containing carbon and is in a gaseous state. For example, the carbon-containing compound gas may include a hydrocarbon compound gas having 1 to 4 carbon atoms (methane, ethane, propane, butane, etc.). This may not be limited.

Hereinafter, this application is demonstrated in detail.

A first aspect of the present disclosure is a liquid tank containing a liquid; A liquid circulation line unit for forcibly circulating a liquid contained in the liquid tank; A gas supply line unit supplying gas to the liquid tank; And installed inside the liquid tank in a state of being connected to the gas supply line part, and when the gas supplied from the gas supply line part is discharged into the micro bubbles, the micro bubbles are vibrated by ultrasonic waves so as not to aggregate with each other. Provided is a microbubble water production apparatus using an ultrasonic vibrator, comprising a plurality of microbubble emitting member to shake off the micro-bubbles.

According to one embodiment of the present application, the liquid may include one selected from the group consisting of water, a high viscosity material, and combinations thereof, but may not be limited thereto. For example, the high viscosity material may be selected from the group consisting of polymer, fuel, and combinations thereof, but may not be limited thereto. For example, the high viscosity material may include one selected from the group consisting of polymers, fossil fuels, biofuels, and combinations thereof, for example, lubricating oil, gasoline, diesel, bunker oil, bioethanol, biomethanol , Biodiesel, and combinations thereof, but may not be limited thereto.

According to the exemplary embodiment of the present application, as shown in FIG. 1, the apparatus for manufacturing microbubble water using the ultrasonic vibrator has a predetermined space formed therein such that the liquid tank 100 receives a predetermined amount of liquid. In the upper part, a viewing window (not shown) capable of confirming the inside may be formed, but may not be limited thereto. An upper surface of the liquid tank 100 may be provided with a check valve (not shown) for checking the pressure in the liquid tank 100 and adjusting the pressure in the liquid tank 100, but is not limited thereto. You may not. In addition, an outlet 101 capable of discharging the liquid contained in the liquid tank 100 and an inlet 102 capable of introducing liquid into the liquid tank 100 may be formed, but may not be limited thereto. For example, the outlet 101 may be formed at a position lower than the level of the liquid contained in the liquid tank 100, but may be formed below the liquid tank 100, but is not limited thereto. Can be. For example, the inlet 102 may include an upper portion or a lower portion of the liquid tank 100, but may not be limited thereto. In addition, a gas injection member through which gas is introduced may be additionally installed on the upper portion of the liquid tank 100, but may not be limited thereto. The gas injection member may include injecting gas into the liquid tank 100 to maintain an atmosphere in the liquid tank 100 at a gas saturation state, and may generate pressure in the liquid tank 100. This may not be limited.

According to the exemplary embodiment of the present application, the liquid circulation line unit 200 circulates the liquid contained in the liquid tank 100, but may include a circulation tube 210 and a circulation motor 220. This may not be limited. The circulation pipe 210 connects the discharge port 101 and the inlet port 102 provided in the liquid tank 100 to discharge the liquid in the liquid tank 100 through the discharge port 101. After that, it may be to be re-introduced into the liquid tank 100 through the inlet 102 to circulate, but may not be limited thereto. In addition, the circulation tube 210 is provided with the circulation motor 220 and is selectively driven according to a control signal applied from the outside, so that the liquid in the liquid tank 100 discharged through the discharge port 101 is discharged. It may be to circulate along the circulation pipe 210 to be forced to be drawn back into the liquid tank 100 through the inlet 102, but may not be limited thereto. Therefore, the liquid circulation line unit 200, the circulation pipe 210 is connected to the outlet 101 and the inlet port 102 of the liquid tank 100 in accordance with a control signal applied from the outside of the circulation pipe 210 The circulation motor 220 is driven to the liquid in the liquid tank 100 is discharged through the discharge port 101, circulated along the circulation pipe 210 to the back through the inlet 102 It may be to circulate to be drawn into the liquid tank 100, but may not be limited thereto.

According to the exemplary embodiment of the present application, the gas supply line unit 300 supplies gas to the liquid tank 100, and a gas cylinder 310, a supply pipe 320, a pressure control valve 330, and a minute It may include a pipe 340, but may not be limited thereto. The gas is selected from the group consisting of hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof It may be to include, but may not be limited thereto. The gas cylinder 310 of the gas supply line 300 is filled with the gas to be dissolved in the liquid tank 100, the gas may be supplied to the internal pressure in the cylinder generated in the gas filled state, but is not limited thereto. It may not be. The gas cylinder 310 may also include a pressure gauge and an opening / closing valve for checking pressure, but may not be limited thereto. The opening and closing valve (not shown) of the gas cylinder 310 may be connected to the supply pipe 320 so that the gas supplied from the gas cylinder 310 may be guided, but may not be limited thereto. The supply pipe 320 is connected to the pressure control valve 330, the pressure of the gas guided through the supply pipe 320 is selectively reduced or pressurized by the pressure control valve 330 to a predetermined uniform The pressure may be adjusted to supply gas, but may not be limited thereto. In addition, the distribution pipe 340 may be provided to distribute the reduced pressure or pressurized gas through the pressure control valve 330, but may not be limited thereto. The gas is injected into the liquid tank 100 through the gas injection member provided on the upper portion of the liquid tank 100 through the distribution pipe 340, or through the distribution pipe 340. The gas may be supplied to the microbubble discharge member 400 installed inside the liquid tank 100, but may not be limited thereto. Therefore, the gas supply line unit 300 is the gas filled in the gas cylinder 310 is supplied by the internal pressure of the cylinder, guided by the supply pipe 320 is a predetermined uniformity by the pressure control valve 330 It may include being distributed by the distribution pipe 340 while maintaining a pressure, the gas of the gas cylinder 310 may be supplied to the gas injection member and the fine bubble discharge member 400, This may not be limited.

2 and 3, the microbubble discharging member 400 is installed in the lower side of the liquid tank 100 in a state connected to the gas supply line part 300, and the distribution pipe 340. The gas injected through the microbubble discharge member 400 may be discharged as a fine bubble, but may not be limited thereto. At this time, the fine bubble discharge member 400 is preferably installed at a position lower than the level of the liquid contained in the liquid tank (100).

According to the exemplary embodiment of the present application, the average diameter of the micro bubbles emitted through the microbubble emitting member 400 may be about 1 nm to about 1,000 μm, but may not be limited thereto. For example, the average diameter of the microbubbles is about 1 nm to about 1,000 μm, about 10 nm to about 1,000 μm, about 100 nm to about 1,000 μm, about 300 nm to about 1,000 μm, about 500 nm to about 1,000 μm , About 700 nm to about 1,000 μm, about 900 nm to about 1,000 μm, about 1 μm to about 1,000 μm, about 10 μm to about 1,000 μm, about 100 μm to about 1,000 μm, about 300 μm to about 1,000 μm, about 500 μm to about 1,000 μm, about 700 μm to about 1,000 μm, about 900 μm to about 1,000 μm, about 1 nm to about 900 μm, about 10 nm to about 900 μm, about 100 nm to about 900 μm, about 300 nm To about 900 μm, about 500 nm to about 900 μm, about 700 nm to about 900 μm, about 900 nm to about 900 μm, about 1 μm to about 900 μm, about 10 μm to about 900 μm, about 100 μm to about 900 μm, about 300 μm to about 900 μm, about 500 μm to about 900 μm, about 700 μm to about 900 μm, about 1 nm to about 700 μm, about 10 nm to about 700 μm, about 100 nm to about 700 μm, about 300 nm to about 700 μm, about 500 nm to about 700 μm, about 700 nm to about 700 μm, about 900 nm to about 700 μm, about 1 μm to about 700 μm, about 10 μm To about 700 μm, about 100 μm to about 700 μm, about 300 μm to about 700 μm, about 500 μm to about 700 μm, about 1 nm to about 500 μm, about 10 nm to about 500 μm, about 100 nm to about 500 μm, about 300 nm to about 500 μm, about 500 nm to about 500 μm, about 700 nm to about 500 μm, about 900 nm to about 500 μm, about 1 μm to about 500 μm, about 10 μm to about 500 μm About 100 μm to about 500 μm, about 300 μm to about 500 μm, about 1 nm to about 300 μm, about 10 nm to about 300 μm, about 100 nm to about 300 μm, about 300 nm to about 300 μm, about 500 nm to about 300 μm, about 700 nm to about 300 μm, about 900 nm to about 300 μm, about 1 μm to about 300 μm, about 10 μm to about 300 μm, about 100 μm to about 300 μm, about 1 nm To about 100 , About 10 nm to about 100 μm, about 100 nm to about 100 μm, about 300 nm to about 100 μm, about 500 nm to about 100 μm, about 700 nm to about 100 μm, about 900 nm to about 100 μm, about 1 μm to about 100 μm, about 10 μm to about 100 μm, about 1 nm to about 10 μm, about 10 nm to about 10 μm, about 100 nm to about 10 μm, about 300 nm to about 10 μm, about 500 nm To about 10 μm, about 700 nm to about 10 μm, about 900 nm to about 10 μm, about 1 μm to about 10 μm, about 1 nm to about 1 μm, about 10 nm to about 1 μm, about 100 nm to about 1 μm, about 300 nm to about 1 μm, about 500 nm to about 1 μm, about 700 nm to about 1 μm, about 900 nm to about 1 μm, about 1 nm to about 900 nm, about 10 nm to about 900 nm , About 100 nm to about 900 nm, about 300 nm to about 900 nm, about 500 nm to about 900 nm, about 700 nm to about 900 nm, about 1 nm to about 700 nm, about 10 nm to about 700 nm, about 100 nm to about 700 nm, about 300 nm About 700 nm, about 500 nm to about 700 nm, about 1 nm to about 500 nm, about 10 nm to about 500 nm, about 100 nm to about 500 nm, about 300 nm to about 500 nm, about 1 nm to about 300 nm, about 10 nm to about 300 nm, about 100 nm to about 300 nm, about 1 nm to about 100 nm, about 10 nm to about 100 nm, or about 1 nm to about 10 nm, but is not limited thereto. You may not.

According to one embodiment of the present application, the microbubble discharge member is a porous tube 410, the porous tube body 410 is formed with the fine pores communicating with the inside so that the gas injected through the distribution pipe 340 is discharged into a fine bubble, the porous tube body 410 It is provided to be connected to one side of, the body portion 420 that is generated by the vibration generated by the ultrasonic vibrator 426 provided therein, and attached to one side of the porous tube 410 in the body portion 420 It may include a vibration transmission member 430 for transmitting the ultrasonic wave propagated to the porous tube 410, but may not be limited thereto.

According to one embodiment of the present application, the porous tube 410 in which the fine pores are formed may have a hole of about 1 nm to about 1 mm, but may not be limited thereto. For example, the porous tube has about 1 nm to about 1 mm, about 10 nm to about 1 mm, about 100 nm to about 1 mm, about 300 nm to about 1 mm, about 500 nm to about 1 mm, about 700 Nm to about 1 mm, about 900 nm to about 1 mm, about 1 μm to about 1 mm, about 10 μm to about 1 mm, about 100 μm to about 1 mm, about 300 μm to about 1 mm, about 500 μm to About 1 mm, about 700 μm to about 1 mm, about 900 μm to about 1 mm, about 1 nm to about 900 μm, about 10 nm to about 900 μm, about 100 nm to about 900 μm, about 300 nm to about 900 Μm, about 500 nm to about 900 μm, about 700 nm to about 900 μm, about 900 nm to about 900 μm, about 1 μm to about 900 μm, about 10 μm to about 900 μm, about 100 μm to about 900 μm, About 300 μm to about 900 μm, about 500 μm to about 900 μm, about 700 μm to about 900 μm, about 1 nm to about 700 μm, about 10 nm to about 700 μm, about 100 nm to about 700 μm, about 300 Nm to about 700 μm, about 500 nm About 700 μm, about 700 nm to about 700 μm, about 900 nm to about 700 μm, about 1 μm to about 700 μm, about 10 μm to about 700 μm, about 100 μm to about 700 μm, about 300 μm to about 700 μm, about 500 μm to about 700 μm, about 1 nm to about 500 μm, about 10 nm to about 500 μm, about 100 nm to about 500 μm, about 300 nm to about 500 μm, about 500 nm to about 500 μm , About 700 nm to about 500 μm, about 900 nm to about 500 μm, about 1 μm to about 500 μm, about 10 μm to about 500 μm, about 100 μm to about 500 μm, about 300 μm to about 500 μm, about 1 nm to about 300 μm, about 10 nm to about 300 μm, about 100 nm to about 300 μm, about 300 nm to about 300 μm, about 500 nm to about 300 μm, about 700 nm to about 300 μm, about 900 nm To about 300 μm, about 1 μm to about 300 μm, about 10 μm to about 300 μm, about 100 μm to about 300 μm, about 1 nm to about 100 μm, about 10 nm to about 100 μm, about 100 nm to about 100 μm, about 300 Nm to about 100 μm, about 500 nm to about 100 μm, about 700 nm to about 100 μm, about 900 nm to about 100 μm, about 1 μm to about 100 μm, about 10 μm to about 100 μm, about 1 nm to About 10 μm, about 10 nm to about 10 μm, about 100 nm to about 10 μm, about 300 nm to about 10 μm, about 500 nm to about 10 μm, about 700 nm to about 10 μm, about 900 nm to about 10 Μm, about 1 μm to about 10 μm, about 1 nm to about 1 μm, about 10 nm to about 1 μm, about 100 nm to about 1 μm, about 300 nm to about 1 μm, about 500 nm to about 1 μm, About 700 nm to about 1 μm, about 900 nm to about 1 μm, about 1 nm to about 900 nm, about 10 nm to about 900 nm, about 100 nm to about 900 nm, about 300 nm to about 900 nm, about 500 Nm to about 900 nm, about 700 nm to about 900 nm, about 1 nm to about 700 nm, about 10 nm to about 700 nm, about 100 nm to about 700 nm, about 300 nm to about 700 nm, about 500 nm to About 700 nm, about 1 nm to about 5 00 nm, about 10 nm to about 500 nm, about 100 nm to about 500 nm, about 300 nm to about 500 nm, about 1 nm to about 300 nm, about 10 nm to about 300 nm, about 100 nm to about 300 nm , About 1 nm to about 100 nm, about 10 nm to about 100 nm, or about 1 nm to about 10 nm in size, but may not be limited thereto.

According to one embodiment of the present application, the porous tube 410 may be further provided with a front closing cap 440 and a rear closing cap 450, but may not be limited thereto. The front closure cap 440 is formed on the front end of the porous tube 410 so that the coupling nut portion 441 is formed on one side so that the coupling nut portion 441 is accommodated inside the front end side of the porous tube 410. Is coupled, in this case may be to be coupled in a state in which a sealing ring (not shown) is located between the porous tube 410 and the front closing cap 440 to maintain the airtightness, but may not be limited thereto. The rear closing cap 450 is formed with a bolt portion 451 on one side, and enters from the rear end side of the porous tube 410 and fastened with the coupling nut portion 441 of the front closing cap 440. To be coupled to the rear end side of the porous tube 410, but may not be limited thereto. At this time, the rear closing cap 450 may also be coupled between the porous tube 410 and the rear closing cap 450 in a state in which a sealing ring (not shown) is positioned to maintain airtightness. . The rear closing cap 450 has a plurality of gas through holes 452 are formed around the bolt 451 around the bolt 451, and the gas introduced through the gas through hole 452 is porous. It may be discharged as microbubbles through the pores of the porous tube body 410 through the inside of the tube body 410, but may not be limited thereto. In addition, the curved groove 453 is formed along the outer circumferential surface of the rear closing cap 450, the rear closing cap 450 having the curved groove 453 may be connected to the front end of the body portion 420, This may not be limited. Vibration transmission member 430 is attached to the outside of the rear closing cap 450 of the porous tube 410 may include transmitting the ultrasonic vibration generated in the body portion 420 to the porous tube 410, but However, this may not be limited. For example, the vibration transmission member 430 may be a vibration pin to be vibrated by the ultrasonic wave toward the outward ultrasonic vibrator 426, the ultrasonic vibration is transmitted to the porous tube 410 by the vibration pin. However, this may not be limited.

 According to the exemplary embodiment of the present application, the body portion 420 may include a main body tube 421, an elastic fixing ring 422, and a connecting tube 423, but may not be limited thereto. The main body tube 421 is formed in a tubular shape having a predetermined diameter, the front end inner peripheral surface is formed with a curved coupling groove 424 when the rear end of the porous tube 410 is connected to the front end of the body portion 420 the rear The curved groove 453 of the closing cap 450 and the curved coupling groove 424 of the main tube 421 may be replaced with each other, but may not be limited thereto. At this time, the elastic fixing ring 422 is provided between the curved groove 453 of the rear closing cap 450 and the curved coupling groove 424 of the main body tube 421 facing each other, the body portion It may include, but is not limited to, the porous tube 410 to be fixed to the flow (420). The elastic fixing ring 422 may be to maintain the airtight between the porous tube 410 and the body 420, as well as fixing the porous tube 410 and the body portion 420. In addition, the rear end of the main body tube 421 is connected to the connecting pipe 423, the center of the connecting pipe 423 is provided with an ultrasonic vibrator 426, around the ultrasonic vibrator 426 around the A gas inlet hole 425 connected to the distribution pipe 340 of the gas supply line part 300 may be formed, but may not be limited thereto.

 The ultrasonic vibrator 426 is the same as a conventional ultrasonic vibrator, a detailed description thereof may be omitted and may be selectively driven by an external control, but may not be limited thereto. For example, the frequency of the ultrasonic vibrator may be about 1 Hz to about 300 MHz, but may not be limited thereto. For example, the frequency of the ultrasonic vibrator is about 1 Hz to about 300 MHz, about 10 Hz to about 300 MHz, about 100 Hz to about 300 MHz, about 300 Hz to about 300 MHz, about 500 Hz to about 300 MHz, About 700 Hz to about 300 MHz, about 900 Hz to about 300 MHz, about 1 Hz to about 300 MHz, about 10 Hz to about 300 MHz, about 100 Hz to about 300 MHz, about 300 Hz to about 300 MHz, about 500 KHz to about 300 MHz, about 700 Hz to about 300 MHz, about 900 Hz to about 300 MHz, about 1 MHz to about 300 MHz, about 10 MHz to about 300 MHz, about 100 MHz to about 300 MHz, about 1 Hz to About 100 MHz, about 10 Hz to about 100 MHz, about 100 Hz to about 100 MHz, about 300 Hz to about 100 MHz, about 500 Hz to about 100 MHz, about 700 Hz to about 100 MHz, about 900 Hz to about 100 MHz, about 1 Hz to about 100 MHz, about 10 Hz to about 100 MHz, about 100 Hz to about 100 MHz, about 300 Hz to about 100 MHz, about 500 Hz to about 100 MHz, about 700 Hz to about 100 MHz , About 900 Hz to about 100 MHz, about 1 MHz to about 100 MHz, about 10 MHz to about 100 MHz, about 1 Hz to about 10 MHz, about 10 Hz to about 10 MHz, about 100 Hz to about 10 MHz, about 300 Hz to about 10 MHz, about 500 Hz to about 10 MHz, about 700 Hz to about 10 MHz, about 900 Hz to about 10 MHz, about 1 Hz to about 10 MHz, about 10 Hz to about 10 MHz, about 100 Hz To about 10 MHz, about 300 Hz to about 10 MHz, about 500 Hz to about 10 MHz, about 700 Hz to about 10 MHz, about 900 Hz to about 10 MHz, about 1 MHz to about 10 MHz, about 1 Hz to about 1 MHz, about 10 Hz to about 1 MHz, about 100 Hz to about 1 MHz, about 300 Hz to about 1 MHz, about 500 Hz to about 1 MHz, about 700 Hz to about 1 MHz, about 900 Hz to about 1 MHz , About 1 Hz to about 1 MHz, about 10 Hz to about 1 MHz, about 100 Hz to about 1 MHz, about 300 Hz to about 1 MHz, about 500 Hz to about 1 MHz, about 700 Hz to about 1 MHz, about 900 Hz to about 1 MHz, about 1 Hz to about 900 Hz, about 10 kPa to about 900 kPa, about 100 kPa to about 900 kPa, about 300 kPa to about 900 kPa, about 500 kPa to about 900 kPa, about 700 kPa to about 900 kPa, about 900 kPa to about 900 kPa, about 1 kPa To about 900 kPa, about 10 kPa to about 900 kPa, about 100 kPa to about 900 kPa, about 300 kPa to about 900 kPa, about 500 kPa to about 900 kPa, about 700 kPa to about 900 kPa, about 1 kPa to about 900 kPa 700 kPa, about 10 kPa to about 700 kPa, about 100 kPa to about 700 kPa, about 300 kPa to about 700 kPa, about 500 kPa to about 700 kPa, about 700 kPa to about 700 kPa, about 900 kPa to about 700 kPa , About 1 kPa to about 700 kPa, about 10 kPa to about 700 kPa, about 100 kPa to about 700 kPa, about 300 kPa to about 700 kPa, about 500 kPa to about 700 kPa, about 1 kPa to about 500 kPa, about 10 kPa to about 500 kPa, about 100 kPa to about 500 kPa, about 300 kPa to about 500 kPa, about 500 kPa to about 500 kPa, about 700 kPa to about 500 kPa, about 900 kPa to about 500 kPa, about 1 kPa To about 500 ㎑, about 10 ㎑ to about 500 ㎑, about 100 ㎑ to about 500 ㎑, about 300 ㎑ to about 500 ㎑, about 1 ㎐ to about 300 ㎑, about 10 ㎐ to about 300 ㎑, about 100 ㎐ to about 300 ㎑, About 300 kPa to about 300 kPa, about 500 kPa to about 300 kPa, about 700 kPa to about 300 kPa, about 900 kPa to about 300 kPa, about 1 kPa to about 300 kPa, about 10 kPa to about 300 kPa, about 100 kPa From about 300 kPa to about 300 kPa, from about 1 kPa to about 100 kPa, from about 10 kPa to about 100 kPa, from about 100 kPa to about 100 kPa, from about 300 kPa to about 100 kPa, from about 500 kPa to about 100 kPa About 100 kPa, about 900 kPa to about 100 kPa, about 1 kPa to about 100 kPa, about 10 kPa to about 100 kPa, about 1 kPa to about 10 kPa, about 10 kPa to about 10 kPa, about 100 kPa to about 10 kPa ㎑, about 300 ㎐ to about 10 500, about 500 ㎐ to about 10 ㎑, about 700 ㎐ to about 10 ㎑, about 900 ㎐ to about 10 ㎑, about 1 ㎑ to about 10 ㎑, about 1 ㎐ to about 1 ㎑, About 10 kPa to about 1 kPa, about 100 kPa to about 1 kPa, about 300 kPa to about 1 kPa, about 500 kPa to about 1 kPa, about 700 kPa to about 1 kPa, about 900 kPa to about 1 kPa, about 1 kPa to about 900 kPa, about 10 kPa To about 900 kPa, about 100 kPa to about 900 kPa, about 300 kPa to about 900 kPa, about 500 kPa to about 900 kPa, about 700 kPa to about 900 kPa, about 1 kPa to about 700 kPa, about 10 kPa to about 700 kPa, about 100 kPa to about 700 kPa, about 300 kPa to about 700 kPa, about 500 kPa to about 700 kPa, about 1 kPa to about 500 kPa, about 10 kPa to about 500 kPa, about 100 kPa to about 500 kPa , From about 300 kPa to about 500 kPa, about 1 kPa to about 300 kPa, about 10 kPa to about 300 kPa, about 100 kPa to about 300 kPa, about 1 kPa to about 100 kPa, about 10 kPa to about 100 kPa, or It may be about 1 kPa to about 10 kPa, but may not be limited thereto.

According to the exemplary embodiment of the present application, the liquid tank 100 may further include a heating device or a cooling device, but may not be limited thereto. The heating device or the cooling device may include being installed inside or outside the liquid tank 100, but may not be limited thereto.

When the liquid tank 100 further includes the heating device, for example, in the manufacture of the microbubbles, high viscosity materials may be difficult to produce microbubbles due to their viscosity. At this time, by further including a heating device in the liquid tank, it is possible to generate a microbubble by lowering the viscosity of the high viscosity material to an appropriate level through temperature control. For example, the heating device may include a heating coil using resistance heat outside the liquid tank, and heat the high viscosity material at an appropriate temperature by flowing a current, but may not be limited thereto. The high viscosity material may be selected from the group consisting of fossil fuels, biofuels, polymers, and combinations thereof, but may not be limited thereto. For example, the high viscosity material may be selected from the group consisting of lubricating oil, polymer, gasoline, diesel, bunker oil, bio ethanol, bio methanol, bio diesel, and combinations thereof, but may not be limited thereto. For example, in the case of the lubricating oil, lubrication characteristics can be improved by generating a fine bubble in the lubricating oil. Conventionally, in order to improve the lubrication characteristics, a method of lowering the viscosity of the lubricating oil through temperature control of the lubricating oil was used. The above method adversely affects the oil film formation of the lubricating oil, resulting in a decrease in the lubricating characteristic. When the microbubble is generated inside the lubricating oil, the solid surface in contact with the polar solvent is negatively charged by the electric double layer effect, so that the liquid around the solid surface has a cation. Since the surface of the microbubble is negatively charged, the microbubble sticks to the solid wall by electrical attraction. This results in the formation of a gas phase around the solid wall surface, which causes a wall slip phenomena between the solid wall and the liquid surface, and the microbubble formed liquid along the gas phase. Move. Therefore, the lubricating oil in which the microbubbles are generated can be smoothly moved compared to the lubricating oil which is not, thereby reducing friction. These friction reducing properties reduce surface wear and heat generation, resulting in relatively better lubrication. In addition, as is commonly known, since the viscosity of the liquid in which the microbubbles are formed has the same viscosity as the liquid in which the microbubbles are not produced, the wall slips while maintaining the viscosity necessary for forming the oil film through the lubricating oil including the microbubbles. By using the phenomenon, it is possible to obtain a lubricant having improved electrical resistance, mechanical strength, heat insulation effect, and lubricating properties as compared with conventional lubricants.

When the liquid tank 100 further includes the cooling device, for example, when the microbubble is formed to improve fuel efficiency, the fuel may be continuously evaporated during the microbubble generation process due to the volatility of the fuel. have. In order to lower the volatility of the fuel, a cooling device may be installed in the liquid tank, or a microbubble may be first generated in the fuel and then the additive may be mixed to lower the volatility of the fuel, but the present invention may not be limited thereto. The fuel may include, but is not limited to, one selected from the group consisting of fossil fuels, biofuels, and combinations thereof. For example, the fossil fuel may include, but is not limited to, one selected from the group consisting of gasoline, diesel, lubricating oil, bunker oil, and combinations thereof. For example, the biofuel may include one selected from the group consisting of bioethanol, biomethanol, biodiesel, and combinations thereof, but may not be limited thereto. When the cooling device is installed in the liquid tank, for example, the cooling device may include being installed inside or outside the liquid tank, but may not be limited thereto. The cooling device may be cooled by a refrigerant circulated through a cooling channel, and the refrigerant may be, for example, air cooling using cold air, but may not be limited thereto. In addition, after the microbubble is first generated in the fuel, the volatility of the fuel may be lowered by a method of mixing an additive, but may not be limited thereto. The volatility of the fuel can be determined not only by the properties of the fuel but also by the additives added to the fuel. For example, when the fuel is gasoline, volatility may be determined according to the mixing of butane, which is dissolved in gasoline and contributes to controlling the vapor pressure of the gasoline and improving octane number. Therefore, after the fine bubble is mixed before butane is mixed, butane may be dissolved to prevent a problem due to volatility of the fuel, but may not be limited thereto.

As described above, the porous tube 410 in which the gas supplied from the outside is discharged as a fine bubble is received and vibrated by the ultrasonic vibration propagated from the ultrasonic vibrator 426, so that the micro bubbles are formed in the porous tube 410. Bubble water can be produced in which the amount of dissolved bubbles of the liquid contained in the liquid tank 100 is maximized by adhering to the surface or desorbed from the porous tube 410 before being agglomerated with each other. The production cost can be drastically reduced by enabling mass production with relatively simple equipment without the need. Residual gas that is not mixed with the liquid is allowed to remain in the liquid tank 100 to maintain the atmosphere in the liquid tank 100 in gas saturation, thereby maximizing the amount of dissolved gas in the bubble water, and is sufficient even over time The amount of dissolved gas can be maintained. In addition, the micro-bubble can be efficiently dissolved in the liquid by forming a very uniform and relatively uniform microbubble, it is possible to achieve the dissolved gas ratio of the bubble water in a relatively short time. On the other hand, by adding a cooling device or a heating device in the microbubble manufacturing apparatus to generate a microbubble inside the high viscosity material and fuel to enhance the properties of high-viscosity materials such as electrical resistance, mechanical strength, insulation effect, lubrication characteristics and energy efficiency of the fuel The improvement effect can be achieved.

The second side of the present application is provided to be connected to one side of the porous tube, the body portion is generated vibration by the ultrasonic vibrator provided therein; And a vibration transmitting member attached to one side of the porous tube to transfer the ultrasonic vibration generated from the body to the porous tube, wherein the ultrasonic vibration is applied to the porous tube through the vibration transmitting member. It provides a microbubble discharge member that is to desorb the micro-bubbles generated in the tube.

According to one embodiment of the present application, the porous tube may have a hole of about 1 nm to about 1 mm, but may not be limited thereto. For example, the porous tube has about 1 nm to about 1 mm, about 10 nm to about 1 mm, about 100 nm to about 1 mm, about 300 nm to about 1 mm, about 500 nm to about 1 mm, about 700 Nm to about 1 mm, about 900 nm to about 1 mm, about 1 μm to about 1 mm, about 10 μm to about 1 mm, about 100 μm to about 1 mm, about 300 μm to about 1 mm, about 500 μm to About 1 mm, about 700 μm to about 1 mm, about 900 μm to about 1 mm, about 1 nm to about 900 μm, about 10 nm to about 900 μm, about 100 nm to about 900 μm, about 300 nm to about 900 Μm, about 500 nm to about 900 μm, about 700 nm to about 900 μm, about 900 nm to about 900 μm, about 1 μm to about 900 μm, about 10 μm to about 900 μm, about 100 μm to about 900 μm, About 300 μm to about 900 μm, about 500 μm to about 900 μm, about 700 μm to about 900 μm, about 1 nm to about 700 μm, about 10 nm to about 700 μm, about 100 nm to about 700 μm, about 300 Nm to about 700 μm, about 500 nm About 700 μm, about 700 nm to about 700 μm, about 900 nm to about 700 μm, about 1 μm to about 700 μm, about 10 μm to about 700 μm, about 100 μm to about 700 μm, about 300 μm to about 700 μm, about 500 μm to about 700 μm, about 1 nm to about 500 μm, about 10 nm to about 500 μm, about 100 nm to about 500 μm, about 300 nm to about 500 μm, about 500 nm to about 500 μm , About 700 nm to about 500 μm, about 900 nm to about 500 μm, about 1 μm to about 500 μm, about 10 μm to about 500 μm, about 100 μm to about 500 μm, about 300 μm to about 500 μm, about 1 nm to about 300 μm, about 10 nm to about 300 μm, about 100 nm to about 300 μm, about 300 nm to about 300 μm, about 500 nm to about 300 μm, about 700 nm to about 300 μm, about 900 nm To about 300 μm, about 1 μm to about 300 μm, about 10 μm to about 300 μm, about 100 μm to about 300 μm, about 1 nm to about 100 μm, about 10 nm to about 100 μm, about 100 nm to about 100 μm, about 300 Nm to about 100 μm, about 500 nm to about 100 μm, about 700 nm to about 100 μm, about 900 nm to about 100 μm, about 1 μm to about 100 μm, about 10 μm to about 100 μm, about 1 nm to About 10 μm, about 10 nm to about 10 μm, about 100 nm to about 10 μm, about 300 nm to about 10 μm, about 500 nm to about 10 μm, about 700 nm to about 10 μm, about 900 nm to about 10 Μm, about 1 μm to about 10 μm, about 1 nm to about 1 μm, about 10 nm to about 1 μm, about 100 nm to about 1 μm, about 300 nm to about 1 μm, about 500 nm to about 1 μm, About 700 nm to about 1 μm, about 900 nm to about 1 μm, about 1 nm to about 900 nm, about 10 nm to about 900 nm, about 100 nm to about 900 nm, about 300 nm to about 900 nm, about 500 Nm to about 900 nm, about 700 nm to about 900 nm, about 1 nm to about 700 nm, about 10 nm to about 700 nm, about 100 nm to about 700 nm, about 300 nm to about 700 nm, about 500 nm to About 700 nm, about 1 nm to about 5 00 nm, about 10 nm to about 500 nm, about 100 nm to about 500 nm, about 300 nm to about 500 nm, about 1 nm to about 300 nm, about 10 nm to about 300 nm, about 100 nm to about 300 nm , About 1 nm to about 100 nm, about 10 nm to about 100 nm, or about 1 nm to about 10 nm in size, but may not be limited thereto.

According to one embodiment of the present application, the porous tube is a front end cap coupled to the coupling nut portion formed on one side inside the front end of the porous tube, and the bolt portion formed on one side inside the rear end of the porous tube body Entering into and coupled to the coupling nut portion of the front closing cap, a plurality of gas through-holes are formed around the bolt portion around the bolt portion, may include a rear closure cap is formed along the outer peripheral surface curved groove However, this may not be limited. For example, the front closure cap is coupled to the front end of the porous tube body so that the coupling nut portion is formed on one side of the front end of the porous tube body to be accommodated in the front end side of the porous tube body, and in order to maintain airtightness with the porous tube body The front closing cap may be coupled in a state where the sealing ring is located, but may not be limited thereto. For example, the rear closure cap is formed with a bolt portion on one side, the rear end cap of the porous tube may be coupled to the rear end side of the porous tube to be engaged with the coupling nut portion of the front closing cap inside. However, this may not be limited. At this time, it may be preferable that the rear closure cap is also coupled between the porous tube body and the rear closure cap in a state in which a sealing ring is positioned to maintain airtightness. The rear finishing cap has a plurality of gas through-holes are formed around the bolts around the bolts, and the gas introduced through the gas through-holes can be discharged into the micro bubbles through the pores of the porous pipes through the inside of the porous pipes. However, this may not be limited. For example, the gas consists of hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof. It may include a gas selected from the group, but may not be limited thereto. In addition, the curved groove is formed along the outer circumferential surface of the rear closing cap, the rear closing cap formed with the curved groove may be connected to the front end of the body portion, but may not be limited thereto. Vibration transmission member is attached to the outside of the rear closing cap of the porous tube may include transmitting the ultrasonic vibration generated in the body portion to the porous tube, but may not be limited thereto. For example, the vibration transmission member may be a vibration pin which is vibrated by the ultrasonic wave toward the ultrasonic vibrator that is outward, so that the ultrasonic vibration is transmitted to the porous tube by the vibration pin, but may not be limited thereto.

According to the exemplary embodiment of the present application, the body portion is formed in a tubular shape, the curved coupling groove is formed along the inner circumferential surface in the front, the front tube formed with the curved coupling groove is fixed to the rear end of the porous tube body, the body It is seated so as to flow between the curved coupling groove of the tube and the curved recess of the rear end cap, the elastic fixing ring is fixed to the porous tube body, and the rear end of the main body screw is screwed, the center is equipped with an ultrasonic vibrator It may include, but is not limited to, a connection tube having a gas inlet hole connected to the gas supply line around the ultrasonic vibrator. The main body tube is formed in a tubular shape having a predetermined diameter, and a curved coupling groove is formed on the inner circumferential surface of the front end so that the curved groove of the rear closing cap and the curved coupling groove of the main tube when the rear end of the porous tube body is connected to the front end of the body portion May be substituted for each other, but may not be limited thereto. At this time, an elastic fixing ring is provided between the curved recessed groove of the rear closing cap facing each other and the curved coupling groove of the main body tube, and may include fixing the porous tube body to be flowable to the body portion. However, this may not be limited. The elastic fixing ring may be to fix the porous tube body and the body portion, as well as to maintain the airtight between the porous tube body and the body portion. In addition, the rear end of the main body tube is screw-coupled, the center of the connecting tube may be provided with an ultrasonic vibrator, but may not be limited thereto.

According to the exemplary embodiment of the present application, the ultrasonic vibrator is the same as a conventional ultrasonic vibrator, and the detailed description may be omitted and may be selectively driven by external control, but may not be limited thereto. For example, the frequency of the ultrasonic vibrator may be about 1 Hz to about 300 MHz, but may not be limited thereto. For example, the frequency of the ultrasonic vibrator is about 1 Hz to about 300 MHz, about 10 Hz to about 300 MHz, about 100 Hz to about 300 MHz, about 300 Hz to about 300 MHz, about 500 Hz to about 300 MHz, About 700 Hz to about 300 MHz, about 900 Hz to about 300 MHz, about 1 Hz to about 300 MHz, about 10 Hz to about 300 MHz, about 100 Hz to about 300 MHz, about 300 Hz to about 300 MHz, about 500 KHz to about 300 MHz, about 700 Hz to about 300 MHz, about 900 Hz to about 300 MHz, about 1 MHz to about 300 MHz, about 10 MHz to about 300 MHz, about 100 MHz to about 300 MHz, about 1 Hz to About 100 MHz, about 10 Hz to about 100 MHz, about 100 Hz to about 100 MHz, about 300 Hz to about 100 MHz, about 500 Hz to about 100 MHz, about 700 Hz to about 100 MHz, about 900 Hz to about 100 MHz, about 1 Hz to about 100 MHz, about 10 Hz to about 100 MHz, about 100 Hz to about 100 MHz, about 300 Hz to about 100 MHz, about 500 Hz to about 100 MHz, about 700 Hz to about 100 MHz , About 900 Hz to about 100 MHz, about 1 MHz to about 100 MHz, about 10 MHz to about 100 MHz, about 1 Hz to about 10 MHz, about 10 Hz to about 10 MHz, about 100 Hz to about 10 MHz, about 300 Hz to about 10 MHz, about 500 Hz to about 10 MHz, about 700 Hz to about 10 MHz, about 900 Hz to about 10 MHz, about 1 Hz to about 10 MHz, about 10 Hz to about 10 MHz, about 100 Hz To about 10 MHz, about 300 Hz to about 10 MHz, about 500 Hz to about 10 MHz, about 700 Hz to about 10 MHz, about 900 Hz to about 10 MHz, about 1 MHz to about 10 MHz, about 1 Hz to about 1 MHz, about 10 Hz to about 1 MHz, about 100 Hz to about 1 MHz, about 300 Hz to about 1 MHz, about 500 Hz to about 1 MHz, about 700 Hz to about 1 MHz, about 900 Hz to about 1 MHz , About 1 Hz to about 1 MHz, about 10 Hz to about 1 MHz, about 100 Hz to about 1 MHz, about 300 Hz to about 1 MHz, about 500 Hz to about 1 MHz, about 700 Hz to about 1 MHz, about 900 Hz to about 1 MHz, about 1 Hz to about 900 Hz, about 10 kPa to about 900 kPa, about 100 kPa to about 900 kPa, about 300 kPa to about 900 kPa, about 500 kPa to about 900 kPa, about 700 kPa to about 900 kPa, about 900 kPa to about 900 kPa, about 1 kPa To about 900 kPa, about 10 kPa to about 900 kPa, about 100 kPa to about 900 kPa, about 300 kPa to about 900 kPa, about 500 kPa to about 900 kPa, about 700 kPa to about 900 kPa, about 1 kPa to about 900 kPa 700 kPa, about 10 kPa to about 700 kPa, about 100 kPa to about 700 kPa, about 300 kPa to about 700 kPa, about 500 kPa to about 700 kPa, about 700 kPa to about 700 kPa, about 900 kPa to about 700 kPa , About 1 kPa to about 700 kPa, about 10 kPa to about 700 kPa, about 100 kPa to about 700 kPa, about 300 kPa to about 700 kPa, about 500 kPa to about 700 kPa, about 1 kPa to about 500 kPa, about 10 kPa to about 500 kPa, about 100 kPa to about 500 kPa, about 300 kPa to about 500 kPa, about 500 kPa to about 500 kPa, about 700 kPa to about 500 kPa, about 900 kPa to about 500 kPa, about 1 kPa To about 500 ㎑, about 10 ㎑ to about 500 ㎑, about 100 ㎑ to about 500 ㎑, about 300 ㎑ to about 500 ㎑, about 1 ㎐ to about 300 ㎑, about 10 ㎐ to about 300 ㎑, about 100 ㎐ to about 300 ㎑, About 300 kPa to about 300 kPa, about 500 kPa to about 300 kPa, about 700 kPa to about 300 kPa, about 900 kPa to about 300 kPa, about 1 kPa to about 300 kPa, about 10 kPa to about 300 kPa, about 100 kPa From about 300 kPa to about 300 kPa, from about 1 kPa to about 100 kPa, from about 10 kPa to about 100 kPa, from about 100 kPa to about 100 kPa, from about 300 kPa to about 100 kPa, from about 500 kPa to about 100 kPa About 100 kPa, about 900 kPa to about 100 kPa, about 1 kPa to about 100 kPa, about 10 kPa to about 100 kPa, about 1 kPa to about 10 kPa, about 10 kPa to about 10 kPa, about 100 kPa to about 10 kPa ㎑, about 300 ㎐ to about 10 500, about 500 ㎐ to about 10 ㎑, about 700 ㎐ to about 10 ㎑, about 900 ㎐ to about 10 ㎑, about 1 ㎑ to about 10 ㎑, about 1 ㎐ to about 1 ㎑, About 10 kPa to about 1 kPa, about 100 kPa to about 1 kPa, about 300 kPa to about 1 kPa, about 500 kPa to about 1 kPa, about 700 kPa to about 1 kPa, about 900 kPa to about 1 kPa, about 1 kPa to about 900 kPa, about 10 kPa To about 900 kPa, about 100 kPa to about 900 kPa, about 300 kPa to about 900 kPa, about 500 kPa to about 900 kPa, about 700 kPa to about 900 kPa, about 1 kPa to about 700 kPa, about 10 kPa to about 700 kPa, about 100 kPa to about 700 kPa, about 300 kPa to about 700 kPa, about 500 kPa to about 700 kPa, about 1 kPa to about 500 kPa, about 10 kPa to about 500 kPa, about 100 kPa to about 500 kPa , From about 300 kPa to about 500 kPa, about 1 kPa to about 300 kPa, about 10 kPa to about 300 kPa, about 100 kPa to about 300 kPa, about 1 kPa to about 100 kPa, about 10 kPa to about 100 kPa, or It may be about 1 kPa to about 10 kPa, but may not be limited thereto.

According to the exemplary embodiment of the present application, the average diameter of the micro bubbles generated in the porous tube may be about 1 nm to about 1,000 μm, but may not be limited thereto. For example, the average diameter of the microbubbles is about 1 nm to about 1,000 μm, about 10 nm to about 1,000 μm, about 100 nm to about 1,000 μm, about 300 nm to about 1,000 μm, about 500 nm to about 1,000 μm , About 700 nm to about 1,000 μm, about 900 nm to about 1,000 μm, about 1 μm to about 1,000 μm, about 10 μm to about 1,000 μm, about 100 μm to about 1,000 μm, about 300 μm to about 1,000 μm, about 500 μm to about 1,000 μm, about 700 μm to about 1,000 μm, about 900 μm to about 1,000 μm, about 1 nm to about 900 μm, about 10 nm to about 900 μm, about 100 nm to about 900 μm, about 300 nm To about 900 μm, about 500 nm to about 900 μm, about 700 nm to about 900 μm, about 900 nm to about 900 μm, about 1 μm to about 900 μm, about 10 μm to about 900 μm, about 100 μm to about 900 μm, about 300 μm to about 900 μm, about 500 μm to about 900 μm, about 700 μm to about 900 μm, about 1 nm to about 700 μm, about 10 nm to about 700 μm, about 100 nm to about 700 μm, about 300 nm to about 700 μm, about 500 nm to about 700 μm, about 700 nm to about 700 μm, about 900 nm to about 700 μm, about 1 μm to about 700 μm, about 10 μm To about 700 μm, about 100 μm to about 700 μm, about 300 μm to about 700 μm, about 500 μm to about 700 μm, about 1 nm to about 500 μm, about 10 nm to about 500 μm, about 100 nm to about 500 μm, about 300 nm to about 500 μm, about 500 nm to about 500 μm, about 700 nm to about 500 μm, about 900 nm to about 500 μm, about 1 μm to about 500 μm, about 10 μm to about 500 μm About 100 μm to about 500 μm, about 300 μm to about 500 μm, about 1 nm to about 300 μm, about 10 nm to about 300 μm, about 100 nm to about 300 μm, about 300 nm to about 300 μm, about 500 nm to about 300 μm, about 700 nm to about 300 μm, about 900 nm to about 300 μm, about 1 μm to about 300 μm, about 10 μm to about 300 μm, about 100 μm to about 300 μm, about 1 nm To about 100 , About 10 nm to about 100 μm, about 100 nm to about 100 μm, about 300 nm to about 100 μm, about 500 nm to about 100 μm, about 700 nm to about 100 μm, about 900 nm to about 100 μm, about 1 μm to about 100 μm, about 10 μm to about 100 μm, about 1 nm to about 10 μm, about 10 nm to about 10 μm, about 100 nm to about 10 μm, about 300 nm to about 10 μm, about 500 nm To about 10 μm, about 700 nm to about 10 μm, about 900 nm to about 10 μm, about 1 μm to about 10 μm, about 1 nm to about 1 μm, about 10 nm to about 1 μm, about 100 nm to about 1 μm, about 300 nm to about 1 μm, about 500 nm to about 1 μm, about 700 nm to about 1 μm, about 900 nm to about 1 μm, about 1 nm to about 900 nm, about 10 nm to about 900 nm , About 100 nm to about 900 nm, about 300 nm to about 900 nm, about 500 nm to about 900 nm, about 700 nm to about 900 nm, about 1 nm to about 700 nm, about 10 nm to about 700 nm, about 100 nm to about 700 nm, about 300 nm About 700 nm, about 500 nm to about 700 nm, about 1 nm to about 500 nm, about 10 nm to about 500 nm, about 100 nm to about 500 nm, about 300 nm to about 500 nm, about 1 nm to about 300 nm, about 10 nm to about 300 nm, about 100 nm to about 300 nm, about 1 nm to about 100 nm, about 10 nm to about 100 nm, or about 1 nm to about 10 nm, but is not limited thereto. You may not.

According to a third aspect of the present invention, there is provided a cell culture medium comprising serum and antibiotic, wherein the cell culture medium contains microbubble water, and about 10 ³ to about 10 ¹⁸ about 1 mL of about 1 mL of the microbubble water. It provides a cell culture medium, comprising a microbubble having an average diameter of nm to about 1,000 μm.

First, the microbubble water may be prepared by a method such as pressure dissolution, rotary liquid flow type, static mixer type, ezekuta type, venturi type, pore type, rotary type, ultrasonic type, steam condensation type, and electrolysis type.

The microbubbles may include microbubbles having an average diameter of about 1 nm to about 1,000 μm, and may include about 10 ³ to about 10 ¹⁸ microbubbles per 1 mL of the microbubbles, but is not limited thereto. It may not be. For example, the average diameter of the microbubbles included in the number of microbubbles, about 1 nm to about 1,000 μm, about 10 nm to about 1,000 μm, about 100 nm to about 1,000 μm, about 300 nm to about 1,000 μm, About 500 nm to about 1,000 μm, about 700 nm to about 1,000 μm, about 900 nm to about 1,000 μm, about 1 μm to about 1,000 μm, about 10 μm to about 1,000 μm, about 100 μm to about 1,000 μm, about 300 Μm to about 1,000 μm, about 500 μm to about 1,000 μm, about 700 μm to about 1,000 μm, about 900 μm to about 1,000 μm, about 1 nm to about 900 μm, about 10 nm to about 900 μm, about 100 nm to About 900 μm, about 300 nm to about 900 μm, about 500 nm to about 900 μm, about 700 nm to about 900 μm, about 900 nm to about 900 μm, about 1 μm to about 900 μm, about 10 μm to about 900 Μm, about 100 μm to about 900 μm, about 300 μm to about 900 μm, about 500 μm to about 900 μm, about 700 μm to about 900 μm, about 1 nm to about 700 μm, about 1 0 nm to about 700 μm, about 100 nm to about 700 μm, about 300 nm to about 700 μm, about 500 nm to about 700 μm, about 700 nm to about 700 μm, about 900 nm to about 700 μm, about 1 μm To about 700 μm, about 10 μm to about 700 μm, about 100 μm to about 700 μm, about 300 μm to about 700 μm, about 500 μm to about 700 μm, about 1 nm to about 500 μm, about 10 nm to about 500 μm, about 100 nm to about 500 μm, about 300 nm to about 500 μm, about 500 nm to about 500 μm, about 700 nm to about 500 μm, about 900 nm to about 500 μm, about 1 μm to about 500 μm About 10 μm to about 500 μm, about 100 μm to about 500 μm, about 300 μm to about 500 μm, about 1 nm to about 300 μm, about 10 nm to about 300 μm, about 100 nm to about 300 μm, about 300 nm to about 300 μm, about 500 nm to about 300 μm, about 700 nm to about 300 μm, about 900 nm to about 300 μm, about 1 μm to about 300 μm, about 10 μm to about 300 μm, about 100 μm To about 300 , About 1 nm to about 100 μm, about 10 nm to about 100 μm, about 100 nm to about 100 μm, about 300 nm to about 100 μm, about 500 nm to about 100 μm, about 700 nm to about 100 μm, about 900 nm to about 100 μm, about 1 μm to about 100 μm, about 10 μm to about 100 μm, about 1 nm to about 10 μm, about 10 nm to about 10 μm, about 100 nm to about 10 μm, about 300 nm To about 10 μm, about 500 nm to about 10 μm, about 700 nm to about 10 μm, about 900 nm to about 10 μm, about 1 μm to about 10 μm, about 1 nm to about 1 μm, about 10 nm to about 1 μm, about 100 nm to about 1 μm, about 300 nm to about 1 μm, about 500 nm to about 1 μm, about 700 nm to about 1 μm, about 900 nm to about 1 μm, about 1 nm to about 900 nm , About 10 nm to about 900 nm, about 100 nm to about 900 nm, about 300 nm to about 900 nm, about 500 nm to about 900 nm, about 700 nm to about 900 nm, about 1 nm to about 700 nm, about 10 nm to about 700 nm, within about 100 nm About 700 nm, about 300 nm to about 700 nm, about 500 nm to about 700 nm, about 1 nm to about 500 nm, about 10 nm to about 500 nm, about 100 nm to about 500 nm, about 300 nm to about 500 nm, about 1 nm to about 300 nm, about 10 nm to about 300 nm, about 100 nm to about 300 nm, about 1 nm to about 100 nm, about 10 nm to about 100 nm, or about 1 nm to about 10 Nm, but may not be limited thereto. The microbubbles are, for example, about 10 ³ to about 10 ¹⁸ , about 10 ⁴ to about 10 ¹⁸ , about 10 ⁵ to about 10 ¹⁸ , about 10 ⁶ per 1 mL of the microbubbles To about 10 ¹⁸ , about 10 ⁷ to about 10 ¹⁸ , about 10 ⁸ to about 10 ¹⁸ , about 10 ⁹ to about 10 ¹⁸ , about 10 ¹⁰ to about 10 ¹⁸ , about 10 ¹¹ To about 10 ¹⁸ , about 10 ¹² to about 10 ¹⁸ , about 10 ¹³ to about 10 ¹⁸ , about 10 ¹⁴ to about 10 ¹⁸ , about 10 ¹⁵ to about 10 ¹⁸ , about 10 ¹⁶ To about 10 ¹⁸ , about 10 ¹⁷ to about 10 ¹⁸ , about 10 ³ to about 10 ¹⁷ , about 10 ⁴ to about 10 ¹⁷ , about 10 ⁵ to about 10 ¹⁷ , about 10 ⁶ To about 10 ¹⁷ , about 10 ⁷ to about 10 ¹⁷ , about 10 ⁸ to about 10 ¹⁷ , about 10 ⁹ to about 10 ¹⁷ , about 10 ¹⁰ to about 10 ¹⁷ , about 10 ¹¹ To about 10 ¹⁷ , about 10 ¹² to about 10 ¹⁷ , about 10 ¹³ to about 10 ¹⁷ , about 10 ¹⁴ to about 10 ¹⁷ , about 10 ¹⁵ to about 10 ¹⁷ , about 10 ¹⁶ to about 10 ¹⁷ , about 10 ³ to about 10 ¹⁶ , about 10 ⁴ to about 10 ¹⁶ , about 10 ⁵ to about 10 ¹⁶ , about 10 ⁶ to about 10 ¹⁶ , about 10 ⁷ to about 10 ¹⁶ , about 10 ⁸ to about 10 ¹⁶ , about 10 ⁹ to about 10 ¹⁶ , about 10 ¹⁰ to about 10 ¹⁶ , about 10 ¹¹ to about 10 ¹⁶ , about 10 ¹² to about 10 ¹⁶ , about 10 ¹³ to about 10 ¹⁶ , about 10 ¹⁴ to about 10 ¹⁶ , about 10 ¹⁵ to about 10 ¹⁶ , about 10 ³ to about 10 ¹⁵ , about 10 ⁴ to about 10 ¹⁵ , about 10 ⁵ to about 10 ¹⁵ , about 10 ⁶ to about 10 ¹⁵ , about 10 ⁷ to about 10 ¹⁵ , about 10 ⁸ to about 10 ¹⁵ , about 10 ⁹ to about 10 ¹⁵ , about 10 ¹⁰ to about 10 ¹⁵ , about 10 ¹¹ to about 10 ¹⁵ , about 10 ¹² to about 10 ¹⁵ , about 10 ¹³ to about 10 ¹⁵ , about 10 ¹⁴ to about 10 ¹⁵ , about 10 ³ To about 10 ¹⁴ , about 10 ⁴ to about 10 ¹⁴ , about 10 ⁵ to about 10 ¹⁴ , about 10 ⁶ to about 10 ¹⁴ , about 10 ⁷ to about 10 ¹⁴ , about 10 ⁸ To about 10 ¹⁴ , about 10 ⁹ to about 10 ¹⁴ , about 10 ¹⁰ to about 10 ¹⁴ , about 10 ¹¹ to about 10 ¹⁴ , about 10 ¹² to about 10 ¹⁴ , about 10 ¹³ To about 10 ¹⁴ , about 10 ³ to about 10 ¹³ , about 10 ⁴ to about 10 ¹³ , about 10 ⁵ to about 10 ¹³ , about 10 ⁶ to about 10 ¹³ , about 10 ⁷ To about 10 ¹³ , about 10 ⁸ to about 10 ¹³ , about 10 ⁹ to about 10 ¹³ , about 10 ¹⁰ to about 10 ¹³ , about 10 ¹¹ to about 10 ¹³ , about 10 ¹² To about 10 ¹³ , about 10 ³ to about 10 ¹² , about 10 ⁴ to about 10 ¹² , about 10 ⁵ to about 10 ¹² , about 10 ⁶ to about 10 ¹² , about 10 ⁷ To about 10 ¹² , about 10 ⁸ to about 10 ¹² , about 10 ⁹ to about 10 ¹² , about 10 ¹⁰ to about 1 0 ¹² , about 10 ¹¹ to about 10 ¹² , about 10 ³ to about 10 ¹¹ , about 10 ⁴ to about 10 ¹¹ , about 10 ⁵ to about 10 ¹¹ , about 10 ⁶ to about 10 ¹¹ , about 10 ⁷ to about 10 ¹¹ , about 10 ⁸ to about 10 ¹¹ , about 10 ⁹ to about 10 ¹¹ , about 10 ¹⁰ to about 10 ¹¹ , about 10 ³ to about 10 ¹⁰ , about 10 ⁴ to about 10 ¹⁰ , about 10 ⁵ to about 10 ¹⁰ , about 10 ⁶ to about 10 ¹⁰ , about 10 ⁷ to about 10 ¹⁰ , about 10 ⁸ to about 10 ¹⁰ , about 10 ⁹ to about 10 ¹⁰ , about 10 ³ to about 10 ⁹ , about 10 ⁴ to about 10 ⁹ , about 10 ⁵ to about 10 ⁹ , about 10 ⁶ to about 10 ⁹ , about 10 ⁷ to about 10 ⁹ , about 10 ⁸ to about 10 ⁹ , about 10 ³ to about 10 ⁸ , about 10 ⁴ to about 10 ⁸ , about 10 ⁵ to about 10 ⁸ , about 10 ⁶ to about 10 ⁸ , about 10 ⁷ to about 10 ⁸ , about 10 ³ to about 10 ⁷ , about 10 ⁴ to About 10 ⁷ , about 10 ⁵ to about 10 ⁷ , about 10 ⁶ to about 10 ⁷ , about 10 ³ to about 10 ⁶ , about 10 ⁴ to about 10 ⁶ , about 10 ⁵ to About 10 ⁶ , about 10 ³ to about 10 ⁵ , about 10 ⁴ to about 10 ⁵ , or about 10 ³ to about 10 ⁴ may be included, but may not be limited thereto. The microbubbles may be present in water for a long time by stabilizing the microbubbles due to the self-pressing effect of the microbubbles. However, when the average diameter of the microbubbles is larger than the range, the buoyancy effect of the bubbles may shorten the time that the microbubbles stay in the water, thereby causing a problem that the dissolved amount in the water is inhibited. In addition, when the number of the microbubbles per 1 mL of the microbubble water is less than about 10 ³ outside the range, due to the number of individuals disappeared by the self-pressure effect of the microbubbles, solubility in water, sterilization of microbubbles, Problems such as surface activity and uniformity of the uniform number of fine bubbles in the bubble may be caused.

According to one embodiment of the present application, the microbubble water may be included in about 1 part by volume to about 50 parts by volume, preferably about 1 part by volume to about 20 parts by volume, based on 100 parts by volume of the cell culture medium. This may not be limited. For example, if the microbubble number is less than about 1 part by volume, a decrease in the number of microbubbles required for cell growth may be caused, whereas when the microbubble number exceeds about 50 parts by volume, Problems such as decreased cell growth may be caused due to reduced nutrients in the cell culture medium.

According to one embodiment of the present application, the microbubble water may apply various gases according to its function, for example, the gas may be hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, It may include, but is not limited to, a gas selected from the group consisting of ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof. The microbubble water may include the gas, but may not be limited thereto.

According to one embodiment of the present application, the cells to which the culture medium can be applied include cancer cells selected from the group consisting of lung cancer cells, prostate cancer cells, gastric cancer cells, breast cancer cells, pancreatic cancer cells, colon cancer cells, and combinations thereof, osteoblasts Cells, kidney cells, fibroblasts, chondrocytes, hepatocytes, nerve cells, muscle cells, stem cells, and combinations thereof may be included, but may not be limited thereto.

According to one embodiment of the present application, the serum may include one selected from the group consisting of fetal bovine serum (FBS), fetal bovine serum (FCS), and combinations thereof, but may not be limited thereto.

A fourth aspect of the present disclosure provides a method of culturing cells comprising culturing the cells confluent in the culture medium; And replacing the culture medium of the cells with a culture medium containing microbubble water, wherein the average of about 10 ³ to about 10 ¹⁸ about 1 nm to about 1,000 μm per about 1 mL of the microbubble water. It provides a cell culture method comprising a microbubble having a diameter.

According to one embodiment of the present invention, the microbubble water is contained in the cell culture medium at about 1 vol. To about 50 vol. The microbubble water may be used to be sterilized under UV before being added to the cell culture medium, but may not be limited thereto.

According to one embodiment of the present invention, the cells are lung cancer cells, prostate cancer cells, gastric cancer cells, breast cancer cells, pancreatic cancer cells, colon cancer cells, cancer cells selected from the group consisting of combinations thereof, osteoblasts, kidney cells, fibroblasts, It may include, but is not limited to, one selected from the group consisting of chondrocytes, hepatocytes, neurons, muscle cells, stem cells, and combinations thereof.

According to one embodiment of the present application, the serum may include one selected from the group consisting of fetal bovine serum (FBS), fetal bovine serum (FCS), and combinations thereof, but may not be limited thereto.

As an example of the present application, as shown in FIGS. 6 to 15, each of lung cancer cells A549, A549D9K, osteoblast MC3T3, fibroblast NIH3T3, and renal cell HEK293 using microbubble-containing cell culture medium. As a result of culturing the cells, it was confirmed that the cell growth was promoted as compared to the control cultured cells using a medium containing no microbubbles.

A fifth aspect of the present application, fuel; And a fine bubble formed in the fuel. In this regard, FIG. 16 is photographed using a high-sensitivity charge coupled device (CCD) camera and an X20 microscope objective lens constituting a nano sight LM10-HS having a microbubble formed in the fuel and an LM14 having a 405 nm laser. As an image of a microbubble, specifically, a photograph of a high-efficiency mixed fuel manufactured according to an embodiment of the present application. According to one embodiment of the present application, the fuel may include one selected from the group consisting of fossil fuel, biofuel, and combinations thereof, but may not be limited thereto. For example, the fossil fuel may include, but is not limited to, one selected from the group consisting of gasoline, diesel, lubricating oil, bunker oil, and combinations thereof. For example, the biofuel may include one selected from the group consisting of bioethanol, biomethanol, biodiesel, and combinations thereof, but may not be limited thereto. For example, the gasoline refers to petroleum oil in a volatile liquid state, which has a high calorific value, good fluidity, fast burning speed, high self-ignition temperature, and low generation of harmful compounds after combustion. However, this may not be limited. For example, the diesel may have a high cetane number and thus have excellent ignition, no impurities, and a large calorific value, but may not be limited thereto. The cetane number is a quantitative value indicating the ignition of the diesel fuel. As the cetane number is higher, it may be more difficult to cause a diesel knock phenomenon, but may not be limited thereto. The microbubbles are filled with a gas filled with an extremely small cavity formed in a liquid. When the microbubbles are used as fuels, the generation of gases exhibiting a greenhouse effect such as carbon monoxide, carbon dioxide, and nitrogen compounds is significantly reduced. In addition, it can be easily transported in the form of gas or liquid, and bulk storage may also be possible. For example, the gas consists of hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof. It may include a gas selected from the group, but may not be limited thereto. For example, in the case of using hydrogen as the gas, the hydrogen may have a number of advantages in terms of efficiency because it can be made of water as a raw material, and can be recycled back to water after use, but may not be limited thereto. .

17A-17D show the concentration and diameter of microbubbles formed in the fuel. For example, as shown in FIG. 17A, the concentration of the microbubbles may increase in number over time through the self fragmentation of the formed microbubbles. For example, the microbubbles may be about 10 ³ to about 10 ¹⁸ microbubbles per 1 mL of the fuel, but may not be limited thereto. For example, the microbubbles are about 10 ³ to about 10 ¹⁸ , about 10 ⁴ to about 10 ¹⁸ , about 10 ⁵ to about 10 ¹⁸ , about 10 ⁶ to about 10 mL of the fuel 10 ¹⁸ , about 10 ⁷ to about 10 ¹⁸ , about 10 ⁸ to about 10 ¹⁸ , about 10 ⁹ to about 10 ¹⁸ , about 10 ¹⁰ to about 10 ¹⁸ , about 10 ¹¹ to about 10 ¹⁸ , about 10 ¹² to about 10 ¹⁸ , about 10 ¹³ to about 10 ¹⁸ , about 10 ¹⁴ to about 10 ¹⁸ , about 10 ¹⁵ to about 10 ¹⁸ , about 10 ¹⁶ to about 10 ¹⁸ , about 10 ¹⁷ to about 10 ¹⁸ , about 10 ³ to about 10 ¹⁷ , about 10 ⁴ to about 10 ¹⁷ , about 10 ⁵ to about 10 ¹⁷ , about 10 ⁶ to about 10 ¹⁷ , about 10 ⁷ to about 10 ¹⁷ , about 10 ⁸ to about 10 ¹⁷ , about 10 ⁹ to about 10 ¹⁷ , about 10 ¹⁰ to about 10 ¹⁷ , about 10 ¹¹ to about 10 ¹⁷ , about 10 ¹² to about 10 ¹⁷ , about 10 ¹³ to about 10 ¹⁷ , about 10 ¹⁴ To about 10 ¹⁷ , about 10 ¹⁵ to about 10 ¹⁷ , about 10 ¹⁶ to about 10 ¹⁷ , about 10 ³ to about 10 ¹⁶ , about 10 ⁴ to about 10 ¹⁶ , about 10 ⁵ To about 10 ¹⁶ , about 10 ⁶ to about 10 ¹⁶ , about 10 ⁷ to about 10 ¹⁶ , about 10 ⁸ to about 10 ¹⁶ , about 10 ⁹ to about 10 ¹⁶ , about 10 ¹⁰ To about 10 ¹⁶ , about 10 ¹¹ to about 10 ¹⁶ , about 10 ¹² to about 10 ¹⁶ , about 10 ¹³ to about 10 ¹⁶ , about 10 ¹⁴ to about 10 ¹⁶ , about 10 ¹⁵ To about 10 ¹⁶ , about 10 ³ to about 10 ¹⁵ , about 10 ⁴ to about 10 ¹⁵ , about 10 ⁵ to about 10 ¹⁵ , about 10 ⁶ to about 10 ¹⁵ , about 10 ⁷ To about 10 ¹⁵ , about 10 ⁸ to about 10 ¹⁵ , about 10 ⁹ to about 10 ¹⁵ , about 10 ¹⁰ to about 10 ¹⁵ , about 10 ¹¹ to about 10 ¹⁵ , about 10 ¹² To about 10 ¹⁵ , about 10 ¹³ to about 10 ¹⁵ , about 10 ¹⁴ to about 10 ¹⁵ , about 10 ³ to About 10 ¹⁴ , about 10 ⁴ to about 10 ¹⁴ , about 10 ⁵ to about 10 ¹⁴ , about 10 ⁶ to about 10 ¹⁴ , about 10 ⁷ to about 10 ¹⁴ , about 10 ⁸ to About 10 ¹⁴ , about 10 ⁹ to about 10 ¹⁴ , about 10 ¹⁰ to about 10 ¹⁴ , about 10 ¹¹ to about 10 ¹⁴ , about 10 ¹² to about 10 ¹⁴ , about 10 ¹³ to About 10 ¹⁴ , about 10 ³ to about 10 ¹³ , about 10 ⁴ to about 10 ¹³ , about 10 ⁵ to about 10 ¹³ , about 10 ⁶ to about 10 ¹³ , about 10 ⁷ to About 10 ¹³ , about 10 ⁸ to about 10 ¹³ , about 10 ⁹ to about 10 ¹³ , about 10 ¹⁰ to about 10 ¹³ , about 10 ¹¹ to about 10 ¹³ , about 10 ¹² About 10 ¹³ , about 10 ³ to about 10 ¹² , about 10 ⁴ to about 10 ¹² , about 10 ⁵ to about 10 ¹² , about 10 ⁶ to about 10 ¹² , about 10 ⁷ to about 10 to ^12, from about 10 ⁸ to about 10 ^12, about 10 ⁹ to about 10 ^12, about 10 ¹⁰ to about 10 ¹² , From about 10 ¹¹ to about 10 ^12, about 10 ³ to about 10 ^11, about 10 ⁴ to about 10 ^11, about 10 ⁵ to about 10 ^11, about 10 ⁶ to about 10 ¹¹ , About 10 ⁷ to about 10 ¹¹ , about 10 ⁸ to about 10 ¹¹ , about 10 ⁹ to about 10 ¹¹ , about 10 ¹⁰ to about 10 ¹¹ , about 10 ³ to about 10 ¹⁰ , About 10 ⁴ to about 10 ¹⁰ , about 10 ⁵ to about 10 ¹⁰ , about 10 ⁶ to about 10 ¹⁰ , about 10 ⁷ to about 10 ¹⁰ , about 10 ⁸ to about 10 ¹⁰ , About 10 ⁹ to about 10 ¹⁰ , about 10 ³ to about 10 ⁹ , about 10 ⁴ to about 10 ⁹ , about 10 ⁵ to about 10 ⁹ , about 10 ⁶ to about 10 ⁹ , About 10 ⁷ to about 10 ⁹ , about 10 ⁸ to about 10 ⁹ , about 10 ³ to about 10 ⁸ , about 10 ⁴ to about 10 ⁸ , about 10 ⁵ to about 10 ⁸ , About 10 ⁶ to about 10 ⁸ , about 10 ⁷ to about 10 ⁸ , about 10 ³ to about 10 ⁷ , about 10 ⁴ to about 10 ⁷ , about 10 ⁵ to about 10 ⁷ , about 10 ⁶ to about 10 ⁷ , about 10 ³ to about 10 ⁶ , about 10 ⁴ to about 10 ⁶ , about 10 ⁵ to about 10 ⁶ , about 10 ³ to about 10 ⁵ , about 10 ⁴ to about 10 ⁵ , or about 10 ³ to about 10 ⁴ may be included, but may not be limited thereto.

Subsequently, referring to FIGS. 17B to 17D, the diameter of the microbubbles may not be uniformly distributed immediately after their formation, but may include microbubbles having a uniform diameter over time, but may not be limited thereto. have. For example, the microbubbles may have a diameter of about 1 nm to about 1,000 μm, but may not be limited thereto. For example, the diameter of the microbubbles may be about 1 nm to about 1,000 μm, about 10 nm to about 1,000 μm, about 100 nm to about 1,000 μm, about 300 nm to about 1,000 μm, about 500 nm to about 1,000 μm, About 700 nm to about 1,000 μm, about 900 nm to about 1,000 μm, about 1 μm to about 1,000 μm, about 10 μm to about 1,000 μm, about 100 μm to about 1,000 μm, about 300 μm to about 1,000 μm, about 500 Μm to about 1,000 μm, about 700 μm to about 1,000 μm, about 900 μm to about 1,000 μm, about 1 nm to about 900 μm, about 10 nm to about 900 μm, about 100 nm to about 900 μm, about 300 nm to About 900 μm, about 500 nm to about 900 μm, about 700 nm to about 900 μm, about 900 nm to about 900 μm, about 1 μm to about 900 μm, about 10 μm to about 900 μm, about 100 μm to about 900 Μm, about 300 μm to about 900 μm, about 500 μm to about 900 μm, about 700 μm to about 900 μm, about 1 nm to about 700 μm, about 10 nm to about 700 μm, about 100 nm To about 700 μm, about 300 nm to about 700 μm, about 500 nm to about 700 μm, about 700 nm to about 700 μm, about 900 nm to about 700 μm, about 1 μm to about 700 μm, about 10 μm to about 700 μm, about 100 μm to about 700 μm, about 300 μm to about 700 μm, about 500 μm to about 700 μm, about 1 nm to about 500 μm, about 10 nm to about 500 μm, about 100 nm to about 500 μm , About 300 nm to about 500 μm, about 500 nm to about 500 μm, about 700 nm to about 500 μm, about 900 nm to about 500 μm, about 1 μm to about 500 μm, about 10 μm to about 500 μm, about 100 μm to about 500 μm, about 300 μm to about 500 μm, about 1 nm to about 300 μm, about 10 nm to about 300 μm, about 100 nm to about 300 μm, about 300 nm to about 300 μm, about 500 nm To about 300 μm, about 700 nm to about 300 μm, about 900 nm to about 300 μm, about 1 μm to about 300 μm, about 10 μm to about 300 μm, about 100 μm to about 300 μm, about 1 nm to about 100 μm, approx. 10 nm to about 100 μm, about 100 nm to about 100 μm, about 300 nm to about 100 μm, about 500 nm to about 100 μm, about 700 nm to about 100 μm, about 900 nm to about 100 μm, about 1 μm To about 100 μm, about 10 μm to about 100 μm, about 1 nm to about 10 μm, about 10 nm to about 10 μm, about 100 nm to about 10 μm, about 300 nm to about 10 μm, about 500 nm to about 10 μm, about 700 nm to about 10 μm, about 900 nm to about 10 μm, about 1 μm to about 10 μm, about 1 nm to about 1 μm, about 10 nm to about 1 μm, about 100 nm to about 1 μm , About 300 nm to about 1 μm, about 500 nm to about 1 μm, about 700 nm to about 1 μm, about 900 nm to about 1 μm, about 1 nm to about 900 nm, about 10 nm to about 900 nm, about 100 nm to about 900 nm, about 300 nm to about 900 nm, about 500 nm to about 900 nm, about 700 nm to about 900 nm, about 1 nm to about 700 nm, about 10 nm to about 700 nm, about 100 nm To about 700 nm, about 300 nm 700 nm, about 500 nm to about 700 nm, about 1 nm to about 500 nm, about 10 nm to about 500 nm, about 100 nm to about 500 nm, about 300 nm to about 500 nm, about 1 nm to about 300 nm , About 10 nm to about 300 nm, about 100 nm to about 300 nm, about 1 nm to about 100 nm, about 10 nm to about 100 nm, or about 1 nm to about 10 nm, but may not be limited thereto. have.

 FIG. 18a shows the viscosity of the fuel and shows the viscosity of the conventional gasoline and gasoline in which microbubbles are formed. The viscosity of the gasoline in which the microbubble is formed may be about 0.4 MPa · s to about 0.7 MPa · s, but may not be limited thereto. For example, the viscosity of the microbubble formed gasoline is about 0.4 MPa · s to about 0.7 MPa · s, about 0.45 MPa · s to about 0.7 MPa · s, about 0.5 MPa · s to about 0.7 MPa · s, about 0.55 MPa · s to about 0.7 MPa · s, about 0.6 MPa · s to about 0.7 MPa · s, about 0.65 MPa · s to about 0.7 MPa · s, about 0.4 MPa · s to about 0.65 MPa · s, about 0.45 MPa S to about 0.65 MPa · s, about 0.5 MPa · s to about 0.65 MPa · s, about 0.55 MPa · s to about 0.65 MPa · s, about 0.6 MPa · s to about 0.65 MPa · s, about 0.4 MPa · s To about 0.6 MPa · s, about 0.45 MPa · s to about 0.6 MPa · s, about 0.5 MPa · s to about 0.6 MPa · s, about 0.55 MPa · s to about 0.6 MPa · s, about 0.4 MPa · s 0.55 MPa · s, about 0.45 MPa · s to about 0.55 MPa · s, about 0.5 MPa · s to about 0.55 MPa · s, about 0.4 MPa · s to about 0.5 MPa · s, about 0.45 MPa · s to about 0.5 MPa S, or about 0.4 MPa · s to about 0.45 MPa · s, but may not be limited thereto. The viscosity of the fuel is an internal resistance that appears when the fuel flows. If the viscosity of the fuel is high, the injection characteristics are deteriorated and the injection pressure must be increased when the fuel is injected, and engine performance and combustion characteristics may be deteriorated, but the present invention is not limited thereto. have.

Meanwhile, FIG. 18B shows the surface tension of the fuel, and shows the surface tension of the gasoline and the gasoline in which the fine bubble is formed. The surface tension of the microbubble formed gasoline may be about 12 dyn / cm to about 15 dyn / cm, but may not be limited thereto. For example, the surface tension of the microbubble formed gasoline is about 12 dyn / cm to about 15 dyn / cm, about 12.5 dyn / cm to about 15 dyn / cm, about 13 dyn / cm to about 15 dyn / cm, About 13.5 dyn / cm to about 15 dyn / cm, about 14 dyn / cm to about 15 dyn / cm, about 14.5 dyn / cm to about 15 dyn / cm, about 12 dyn / cm to about 14.5 dyn / cm, about 12.5 dyn / cm to about 14.5 dyn / cm, about 13 dyn / cm to about 14.5 dyn / cm, about 13.5 dyn / cm to about 14.5 dyn / cm, about 14 dyn / cm to about 14.5 dyn / cm, about 12 dyn / cm to about 14 dyn / cm, about 12.5 dyn / cm to about 14 dyn / cm, about 13 dyn / cm to about 14 dyn / cm, about 13.5 dyn / cm to about 14 dyn / cm, about 12 dyn / cm to About 13.5 dyn / cm, about 12.5 dyn / cm to about 13.5 dyn / cm, about 13 dyn / cm to about 13.5 dyn / cm, about 12 dyn / cm to about 13 dyn / cm, about 12.5 dyn / cm to about 13 dyn / cm, or about 12 dyn / cm to about 12.5 dyn / cm, but may not be limited thereto. The surface tension of the fuel is a force acting on surrounding molecules to reduce the surface occupied by the fuel to the maximum, and may help smooth flow of the fuel according to the adjustment of the surface tension, but may not be limited thereto. have.

A sixth aspect of the present disclosure includes a liquid tank into which a liquid is injected; A gas supply line unit supplying gas to the liquid tank; And it provides a high-efficiency mixed fuel manufacturing apparatus comprising a porous tube installed inside the liquid tank.

19 is a high efficiency mixed fuel manufacturing apparatus using a microbubble according to an embodiment of the present application. First, a liquid tank 510 into which a liquid is injected is prepared. The liquid tank 510 may be a form in which a predetermined liquid is injected and the injected liquid does not leak, like the normal liquid tank, but may not be limited thereto. The liquid tank 510 may include, but is not limited to, an upper portion of the liquid tank 510 sealed by a sealing lid. The airtight lid includes a gas supply line part connected to the airtight lid, a pressure gauge provided in the airtight lid to measure the pressure in the liquid tank 510, and a pressure control valve to control the pressure inside the liquid tank 510. It may be included, but may not be limited thereto. For example, the liquid may include, but is not limited to, one selected from the group consisting of water, high viscosity materials, and combinations thereof. For example, the high viscosity material may be selected from the group consisting of polymer, fuel, and combinations thereof, but may not be limited thereto. For example, the high viscosity material may include one selected from the group consisting of polymers, fossil fuels, biofuels, and combinations thereof, for example, lubricating oil, gasoline, diesel, lubricating oil, bunker oil, bio ethanol, Bio methanol, bio diesel, and combinations thereof may be selected from the group consisting of, but may not be limited thereto.

According to the exemplary embodiment of the present application, the gas supply line part includes an inlet valve 525 into which gas is injected, a pressure gauge measuring a pressure of the injected gas, and a supply pipe 520 through which the injected gas moves. It may be, but may not be limited thereto. For example, the gas consists of hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof. It may include a gas selected from the group, but may not be limited thereto. The gas supply line part may further include a gas tank connected to the inlet valve 525, but may not be limited thereto. For example, by opening the inlet valve 525, the gas contained in the gas tank may be injected into the liquid tank 510 through the supply pipe 520, and injected through the supply pipe 520. The gas may be supplied to the porous tube 530, but may not be limited thereto.

For example, when the gas stored in the gas tank is included in the liquid, the gas generates water after combustion, does not generate pollutants, and can be used efficiently because of its large combustion rate. It may not be limited.

According to one embodiment of the present application, the porous tube 530 may have a hole of about 1 nm to about 1 mm, but may not be limited thereto. For example, the porous tube has about 1 nm to about 1 mm, about 10 nm to about 1 mm, about 100 nm to about 1 mm, about 300 nm to about 1 mm, about 500 nm to about 1 mm, about 700 Nm to about 1 mm, about 900 nm to about 1 mm, about 1 μm to about 1 mm, about 10 μm to about 1 mm, about 100 μm to about 1 mm, about 300 μm to about 1 mm, about 500 μm to About 1 mm, about 700 μm to about 1 mm, about 900 μm to about 1 mm, about 1 nm to about 900 μm, about 10 nm to about 900 μm, about 100 nm to about 900 μm, about 300 nm to about 900 Μm, about 500 nm to about 900 μm, about 700 nm to about 900 μm, about 900 nm to about 900 μm, about 1 μm to about 900 μm, about 10 μm to about 900 μm, about 100 μm to about 900 μm, About 300 μm to about 900 μm, about 500 μm to about 900 μm, about 700 μm to about 900 μm, about 1 nm to about 700 μm, about 10 nm to about 700 μm, about 100 nm to about 700 μm, about 300 Nm to about 700 μm, about 500 nm About 700 μm, about 700 nm to about 700 μm, about 900 nm to about 700 μm, about 1 μm to about 700 μm, about 10 μm to about 700 μm, about 100 μm to about 700 μm, about 300 μm to about 700 μm, about 500 μm to about 700 μm, about 1 nm to about 500 μm, about 10 nm to about 500 μm, about 100 nm to about 500 μm, about 300 nm to about 500 μm, about 500 nm to about 500 μm , About 700 nm to about 500 μm, about 900 nm to about 500 μm, about 1 μm to about 500 μm, about 10 μm to about 500 μm, about 100 μm to about 500 μm, about 300 μm to about 500 μm, about 1 nm to about 300 μm, about 10 nm to about 300 μm, about 100 nm to about 300 μm, about 300 nm to about 300 μm, about 500 nm to about 300 μm, about 700 nm to about 300 μm, about 900 nm To about 300 μm, about 1 μm to about 300 μm, about 10 μm to about 300 μm, about 100 μm to about 300 μm, about 1 nm to about 100 μm, about 10 nm to about 100 μm, about 100 nm to about 100 μm, about 300 Nm to about 100 μm, about 500 nm to about 100 μm, about 700 nm to about 100 μm, about 900 nm to about 100 μm, about 1 μm to about 100 μm, about 10 μm to about 100 μm, about 1 nm to About 10 μm, about 10 nm to about 10 μm, about 100 nm to about 10 μm, about 300 nm to about 10 μm, about 500 nm to about 10 μm, about 700 nm to about 10 μm, about 900 nm to about 10 Μm, about 1 μm to about 10 μm, about 1 nm to about 1 μm, about 10 nm to about 1 μm, about 100 nm to about 1 μm, about 300 nm to about 1 μm, about 500 nm to about 1 μm, About 700 nm to about 1 μm, about 900 nm to about 1 μm, about 1 nm to about 900 nm, about 10 nm to about 900 nm, about 100 nm to about 900 nm, about 300 nm to about 900 nm, about 500 Nm to about 900 nm, about 700 nm to about 900 nm, about 1 nm to about 700 nm, about 10 nm to about 700 nm, about 100 nm to about 700 nm, about 300 nm to about 700 nm, about 500 nm to About 700 nm, about 1 nm to about 5 00 nm, about 10 nm to about 500 nm, about 100 nm to about 500 nm, about 300 nm to about 500 nm, about 1 nm to about 300 nm, about 10 nm to about 300 nm, about 100 nm to about 300 nm , About 1 nm to about 100 nm, about 10 nm to about 100 nm, or about 1 nm to about 10 nm in size, but may not be limited thereto. For example, as shown in FIG. 20, the porous tube may be converted into microbubbles while passing through the porous tube, but may not be limited thereto. For example, when the gas is formed as a micro bubble while passing through the hole of the porous tube, the micro bubble may be converted into nanobubbles due to the self-shrink effect of the micro bubble, but may not be limited thereto. .

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the present application may not be limited by these examples.

Example 1 Preparation of Nanobubble Water

In order to generate the cell culture medium according to the present Example, nanobubbles were first prepared.

1) Hydrogen nano bubble water production

Hydrogen nanobubble water was prepared by a pressure dissolving method by using a microbubble water producing apparatus using an ultrasonic vibrator according to FIG. 1 without applying ultrasonic waves, and injecting gas using a microporous filter.

As a result of performing the prepared hydrogen nanobubble number by nano particle tracking analysis (NTA, LM10-HSBFT14, UK) method, as shown in FIGS. 4A and 4B, The average diameter was about 87 nm and the concentration was about 2.12 × 10 ¹⁷ per about mL.

2) Oxygen Nano Bubble Water Manufacture

Oxygen nanobubble water was prepared by a pressure dissolving method by using a microbubble water producing apparatus using an ultrasonic vibrator according to FIG.

The water quality test of the prepared oxygen nanobubble water was carried out by a nanoparticle tracking analysis (NTA, LM10-HSBFT14, UK) method. As shown in FIGS. 5A and 5B, the nanobubble included in the oxygen nanobubble water The average diameter of the oxygen bubbles was about 87 nm and the concentration was about 2.62 × 10 ¹⁷ per about mL.

Example 2 Cell Culture

In the cell culture method according to the present embodiment, first, four cell lines were first dispensed into each medium, and confluently cultured in a 5% CO ₂ incubator at 37 ° C. In this case, lung cancer cell A549 (purchased from ATCC) contains 10% FetalClone III (Lonza) and 1% penicillin-streptomycin (MP) in DMEM (Cellgro), and A549D9K (D9K mutated CXCR2 expressed in A549) is DMEM (Cellgro). ) Contains 10% FetalClone III (Lonza), 1% penicillin-streptomycin (MP) and 600 μg / mL G418 (Cellgro), osteoblasts MC3T3 (purchased from ATCC) 10% in MEM Alpha Modification (HyClone) Contain fetal calf serum (FBS, Lonza) and 1% penicillin-streptomycin (MP), fibroblast NIH3T3 (purchased from ATCC) and renal cell HEK293 (purchased from ATCC) in DMEM (Cellgro) with 10% fetal calf serum (FBS) , Lonza) and 1% penicillin-streptomycin (MP).

Example 3 Cell Growth and Survival Assay

1. Cell Growth Assay

Cells were separated and passaged using trypsin-EDTA (1 ×, GibcoBRL). To investigate cell viability, optical image cell counting was used by dispensing cells in 24-well culture dishes and 96 well culture dishes at a cell density of about ⁴ × 10 ⁴ cells / mL. At this time, Image-J (Wayne Rasband) was used for cell counting. After 1 day of cell division, the cell medium was incubated at various times by replacing with medium containing 5% by volume, 10% by volume, and 20% by volume of each nanobubble water. At this time, the nanobubble water was used after UV sterilization before mixing in the medium. On the other hand, cells containing only the medium was used as a negative control.

As a result, as shown in Figures 6, 8, 10, 12, and 14, it was confirmed that cell growth increased in lung cancer cells A549, A549D9K, osteoblast MC3T3, fibroblast NIH3T3, and renal cell HEK293, respectively. Could.

2. Optical Growth Rate Analysis

An optical measurement system was used, including a phase contrast microscope (Nikon TiU), camera (Quantiem: 512SC) and NIS-element software (Nikon Instruments Inc.). After replacing with a medium containing nanobubble water, images (15 ×) of each cell were analyzed in each well every given time.

Figures 7, 9, 11, 13, and 15 of the lung cancer cells A549, A549D9K, osteoblasts MC3T3, fibroblasts NIH3T3, and kidney cells HEK293, respectively, showing the cell images, 72 in a medium using nanobubble number 72 In the case of time treatment, cell growth was significantly increased compared to the control group.

Example 4 Preparation of Nanohydrogen Bubble Gasoline

In order to prepare a high-efficiency mixed fuel according to this embodiment, first, nanohydrogen gasoline was manufactured. In order to manufacture the nanohydrogen gasoline, hydrogen gas having a purity of 99.995% (Shinyoung Special Gas) and gasoline having a octane number of 91 to 94 (Hyundai Oil Bank) were used, and no further purification process was performed. To produce nano-hydrogen bubbles in the gasoline was used a microbubble manufacturing apparatus having the same structure as the manufacturing apparatus according to an embodiment of the present application (Fig. 19). The microbubble manufacturing apparatus includes a liquid tank 510 and a porous tube 530 into which gasoline is injected, and the hydrogen gas flowing out of the hydrogen gas tank through the hydrogen gas supply line to the porous tube 530. Injected. The porous tube 530 includes a porous material and is installed below the liquid tank 510 to be immersed in the gasoline. The hydrogen gas injected into the porous tube 530 formed nano hydrogen bubbles on the surface of the porous tube 530. The nanohydrogen bubble formed on the surface of the porous tube 530 has a holding force for holding the nanohydrogen bubble on the solid surface and a detaching force for separating the nanohydrogen bubble from the solid surface. As shown in FIG. 20, as the nanohydrogen bubble grows, the separation force becomes larger than the holding force, and thus the nanohydrogen bubble may be separated from the surface of the porous tube 530. In Example 4, the production of gasoline formed with nano hydrogen bubbles at room temperature and atmospheric pressure was performed, and the amount of evaporated gas was charged due to the high volatility of gasoline during the production of the nano hydrogen bubbles. Finally, after stopping the microbubble manufacturing apparatus, a gasoline in which the hydrogen hydrogen bubbles were formed in the liquid tank 510 was obtained through an outlet valve 540, and the obtained gasoline in which the hydrogen hydrogen bubbles were formed was normal at room temperature and atmospheric pressure. Stored in a plastic bottle.

The experimental engine used in the experiment was a Hyundai EF Sonata using an electronically controlled engine with a displacement of about 2,000 cc in series four-cylinder.

Table 1

As shown in Table 1, the gasoline used in Example 4 is to form a nano-hydrogen bubble in the gasoline (modern oil bank) and the gasoline is commercially available in Korea. Through the measurement of the calorific value of the two gasoline was to determine the effect of the nano-hydrogen bubble on the calorific value of the gasoline, through the results of this experiment it was confirmed that there is no significant difference in the calorific value even if the nano-hydrogen bubble in the existing gasoline.

TABLE 2

Table 2 shows the viscosity of the existing gasoline and gasoline formed nano-hydrogen bubble. As shown in Table 2, the average viscosity of conventional gasoline is about 0.58 MPa · s, and the average viscosity of gasoline that forms nanohydrogen bubbles is about 0.55 MPa · s, and the viscosity of gasoline that forms nanohydrogen bubbles is about 0.03. It was found that MPa · s was lower. An LVT viscometer (Brookfield Engineering Laboratories INC., USA) was used to measure the viscosity of the gasoline. The viscometer used in the measurement includes a body with an indicator needle, a spindle, a pedestal, and a circular level, from about 1.0 MPa · s to about 2.0 at a spindle speed of about 0.3 rpm to about 60 rpm. The viscosity up to × 10 ⁶ MPa · s can be measured. The result was obtained after the guide stabilized at the set spindle speed. Finally, the kinematic viscosity was calculated using the conversion factor when the value indicated by the guide was determined. Viscosity of the gasoline is an internal resistance that appears when the gasoline flows, when the viscosity of the gasoline is high, the injection characteristics deteriorate to increase the injection pressure during fuel injection, engine performance and combustion characteristics may be deteriorated.

TABLE 3

Table 3 shows the surface tension of the conventional gasoline and gasoline formed with nano hydrogen bubbles. The surface tension of the gasoline was measured using a Du Nouy tension meter (Du Nouy tension meter, No.3179, Itoh Seisakusho, Japan) using the Du Nouy ring method for measuring the surface tension of the liquid. . As shown in Table 3, the average surface tension of the existing gasoline is about 13.54 dyn / cm, the average surface tension of the gasoline forming the nano-hydrogen bubble is about 13.93 dyn / cm, the surface tension of the gasoline forming the nano-hydrogen bubble It was found that about 0.39 dyn / cm higher.

Table 4

Referring to Table 4, the average value of the nano-hydrogen bubbles and the number of population changes over time according to the time of the nano-hydrogen gasoline is formed. In order to measure the population, a nano particle tracking analysis (NTA) device was used, and the device irradiated a laser to record an image of a nano hydrogen bubble moving by Brownian motion in a liquid. As a device, the laser irradiation device is mounted under a microscope lens, and nanohydrogen bubbles in the liquid specimen that pass through the laser beam path are represented by small white dots that move or vibrate by the device. Once the image was recorded, NTA 2.3 analytic software tracked each nanohydrogen bubble and measured the diffusion coefficient (D _t ) of the nanohydrogen bubble. As a result, the population of the nanohydrogen bubble was determined by the diffusion coefficient whose radius (r) of the nanohydrogen bubble was measured and the Stokes-Einstine formula represented by the following formula.

D _t = (K _B * T) / (6 * π * η * r)

K _B is Boltzmann's constant, T is temperature, and η is the viscosity of the liquid.

The concentration of gasoline in which the nanohydrogen bubbles formed by the above-described method did not change significantly over time, indicating the stability of the nanohydrogen bubbles in gasoline.

21A to 21C are diagrams illustrating power characteristics of a conventional gasoline and a gasoline in which nano hydrogen bubbles according to the fourth embodiment are formed. 21A to 21C illustrate changes in torque values, fuel consumption rates, and power values measured while increasing the amount of change in the accelerator while the engine rotation speed is fixed at about 3,000 rpm. FIG. 21B illustrates a brake specific fuel consumption according to the engine load according to the present embodiment, and FIG. 21C illustrates the engine according to the present embodiment. It shows the power value according to the load. First, as shown in FIG. 21A, the torque (output) value is a representative value for evaluating output characteristics. In the case of gasoline with a hydrogen hydrogen bubble, the torque value was increased in a low load and a heavy load region compared to a conventional gasoline. In particular, as shown in the fuel consumption rate characteristics as shown in Figure 21b, in the case of the gasoline formed with hydrogen nano-bubble, while showing a smaller amount of fuel consumption improved output value at the same time. This is thought to be the result of higher combustion pressure due to the high explosive pressure characteristics of the hydrogen content in the hydrogen gasoline formed nano hydrogen bubble. As shown in FIG. 21C, the gasoline in which the nano-hydrogen bubble is formed according to the present embodiment shows improved power while consuming a small amount of fuel as compared to the conventional gasoline.

22A to 22D show the harmful exhaust emission characteristics according to the engine load of the conventional gasoline and the gasoline in which the nanohydrogen bubble is formed according to the fourth embodiment, and shows the generation amount of carbon monoxide, carbon dioxide, hydrocarbons, and nitrogen compounds. The harmful exhaust emissions are known as respiratory diseases and major greenhouse gases, and these harmful exhaust emissions are closely related to combustion performance. First, in the case of carbon monoxide and carbon dioxide of FIGS. 22A and 22B, it was found that combustion of gasoline in which nano hydrogen bubbles are formed is lower than that of general gasoline in the overall engine load region. In addition, in the case of the hydrocarbon of Figure 22c, gasoline and nano-hydrogen gasoline formed gasoline in all areas it was found that the amount of hydrocarbon generated is significantly lower than conventional gasoline combustion. The principle of hydrocarbon generation is that carbon and hydrogen, which are the main components of fuel, are not emitted during the combustion process, and as shown in FIG. 22C, in the case of gasoline formed with nanohydrogen bubbles, combustion of hydrocarbons is more complete than that of gasoline. It was also thought to be reduced. Finally, in the case of nitrogen oxide as shown in FIG. 22d, it can be seen that the amount of the compound decreases in the overall engine load region. The nitrogen oxides are directly related to the combustion temperature, and as the combustion pressure increases, the combustion temperature increases, thereby increasing the nitrogen oxides. That is, the amount of nitrogen oxide is also increased as the flame temperature increases. As can be seen from the above power characteristics, high flame temperature caused a high flame temperature in the combustion chamber, which was thought to increase nitrogen oxides. However, the engine used in this experiment is a state in which the exhaust aftertreatment is removed, and in general, since the nitrogen oxides can be easily reduced to the three-way catalytic aftertreatment purification device, the use of the aftertreatment device or the ignition timing or air-fuel ratio (fuel injection amount) It would be possible to reduce it sufficiently if the

In other words, by finding the optimum air-fuel ratio of gasoline and nano-hydrogen mixed fuels and precisely adjusting the ignition timing and fuel injection value, it is possible to simultaneously achieve high output, fuel consumption, and low emissions. .

The above description of the present application is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present application. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present application is indicated by the following claims rather than the above description, and it should be construed that all changes or modifications derived from the meaning and scope of the claims and their equivalents are included in the scope of the present application.

100: liquid tank 101: outlet 102: inlet

200: liquid circulation line portion 210: circulation tube 220: circulation motor

300: gas supply line portion 310: gas cylinder 320: supply pipe

330: pressure control valve 340: distribution pipe 400: fine bubble discharge member

410: porous tube 420: body portion 421: body tube

422: elastic fixing ring 423: connector 424: curved coupling groove

425: gas inlet hole 426: ultrasonic vibrator 430: vibration transmission member

440: front cap 441: coupling nut 450: rear cap

451: bolt portion 452: gas through hole 453: curved groove 510: liquid tank

520: supply pipe 525: inlet valve 530: porous tube 540: outlet valve

Claims (31)

A liquid tank in which a liquid is accommodated; A liquid circulation line unit for forcibly circulating a liquid contained in the liquid tank; A gas supply line unit supplying gas to the liquid tank; And, Installed inside the liquid tank in a state of being connected with the gas supply line part, when the gas supplied from the gas supply line part is discharged as microbubbles, the microbubbles are vibrated by ultrasonic waves so that the microbubbles do not aggregate with each other. A plurality of fine bubble release member to shake off A device comprising a microbubble water using an ultrasonic vibrator. The method of claim 1, The liquid circulation line unit may include a circulation pipe configured to connect an outlet through which the liquid contained in the liquid tank is discharged, and an inlet through which the liquid is introduced into the liquid tank to circulate the liquid discharged through the outlet into the inlet; And, A circulation motor installed in the circulation pipe to force circulation of the liquid A device comprising a microbubble water using an ultrasonic vibrator. The method of claim 1, The gas supply line part Gas cylinder for supplying the gas filled therein at an internal pressure; A supply pipe connected to the gas cylinder to guide the gas supplied from the gas cylinder; A pressure regulating valve connected to the supply pipe to selectively reduce or reduce pressure of the gas supplied through the supply pipe; And, A gas injection member connected to the pressure regulating valve and installed at the upper portion of the liquid tank to inject gas into the tank, the gas being pressurized or pressurized through the pressure regulating valve; Distribution pipes to be supplied to each of the resulting fine bubble discharge member A device comprising a microbubble water using an ultrasonic vibrator. The method of claim 1, The fine bubble discharge member A porous tube body having fine pores communicating therewith such that gas injected through the distribution pipe is discharged into fine bubbles; A body part provided to be connected to one side of the porous tube body and generating vibration by an ultrasonic vibrator provided therein; And, Vibration transmission member attached to one side of the porous tube to transfer the ultrasonic vibration generated in the body portion to the porous tube A microbubble water production apparatus comprising a. The method of claim 4, wherein The porous tube is A front closing cap coupled to the coupling nut formed on one side to be received inside the front end of the porous tube; And, The bolt portion formed on one side enters into the interior from the rear end of the porous tube and is coupled to be engaged with the coupling nut portion of the front closing cap, and a plurality of gas through holes are formed around the bolt portion around the bolt portion, and the outer circumferential surface Closing cap with curved grooves A device comprising a microbubble water using an ultrasonic vibrator. The method of claim 4, wherein The body portion It is formed in a tubular shape, the curved coupling groove is formed along the inner circumferential surface in the front, the front tube formed with the curved coupling groove is inserted into the rear end of the porous tube body is fixed; An elastic fixing ring seated to flow between the curved coupling groove of the main body tube and the curved recess of the rear end cap, so that the porous tube body is fixed to the main body tube; And, A screw connected to the rear end of the main body pipe, the center is provided with an ultrasonic vibrator, the connection pipe body formed with a gas inlet hole connected to the gas supply line around the ultrasonic vibrator around the center A device comprising a microbubble water using an ultrasonic vibrator. The method of claim 1, Wherein the liquid tank further comprises a heating device or a cooling device, fine bubble water production apparatus using an ultrasonic vibrator. The method of claim 7, wherein The heating device or the cooling device is to include installed inside or outside the liquid tank, fine bubble water production apparatus using an ultrasonic vibrator. A body part provided to be connected to one side of the porous tube and generating vibration by an ultrasonic vibrator provided therein; And, Vibration transmission member attached to one side of the porous tube to transfer the ultrasonic vibration generated in the body portion to the porous tube Includes, wherein the ultrasonic vibration is applied to the porous tube through the vibration transmission member to desorb the microbubbles generated in the porous tube, microbubble emitting member. The method of claim 9, The porous tube is A front closing cap coupled to the coupling nut formed on one side to be received inside the front end of the porous tube; And, The bolt portion formed on one side enters into the interior from the rear end of the porous tube and is coupled to be engaged with the coupling nut portion of the front closing cap, and a plurality of gas through holes are formed around the bolt portion around the bolt portion, and the outer circumferential surface Closing cap with curved grooves Containing, microbubble emitting member using an ultrasonic vibrator. The method of claim 9, The body portion It is formed in a tubular shape, the curved coupling groove is formed along the inner circumferential surface in the front, the front tube formed with the curved coupling groove is inserted into the rear end of the porous tube body is fixed; An elastic fixing ring seated to flow between the curved coupling groove of the main body tube and the curved recess of the rear end cap, so that the porous tube body is fixed to the main body tube; And, A screw connected to the rear end of the main body pipe, the center is provided with an ultrasonic vibrator, the connection pipe body formed with a gas inlet hole connected to the gas supply line around the ultrasonic vibrator around the center A microbubble emitting member using an ultrasonic vibrator comprising a. In a cell culture medium containing serum and antibiotics, Wherein the cell culture medium contains microbubbles and comprises microbubbles having an average diameter of 10 ³ to 10 ¹⁸ 1 nm to 1,000 μm per mL of the microbubbles, Cell culture medium. The method of claim 12, The microbubble number is 1 to 50 parts by volume based on 100 parts by weight of the cell culture medium, cell culture medium. The method of claim 12, The fine bubble water is hydrogen, oxygen, carbon dioxide, carbon monoxide, nitrogen, xenon, argon, neon, air, ozone, krypton, helium, nitrogen-containing compound gas, carbon-containing compound gas, and combinations thereof Cell culture medium comprising the selected gas. The method of claim 12, The cells are cancer cells, osteoblasts, kidney cells, fibroblasts, chondrocytes, hepatocytes, neurons, selected from the group consisting of lung cancer cells, prostate cancer cells, gastric cancer cells, breast cancer cells, pancreatic cancer cells, colon cancer cells, and combinations thereof A cell culture medium comprising cells selected from the group consisting of muscle cells, stem cells, and combinations thereof. The method of claim 12, Wherein the serum comprises one selected from the group consisting of fetal bovine serum (FBS), fetal bovine serum (FCS), and combinations thereof. Culturing the cells confluent in the culture medium; And, Replacing the culture medium of the cells with a culture medium containing microbubble water, Cell culture method comprising a microbubble having an average diameter of 10 ³ to 10 ¹⁸ 1 nm to 1,000 ㎛ per 1 mL of the microbubble number. The method of claim 17, The microbubble water is 1 to 50 parts by volume based on 100 parts by weight of the cell culture medium, cell culture method. The method of claim 17, The cells are cancer cells, osteoblasts, kidney cells, fibroblasts, chondrocytes, hepatocytes, neurons, selected from the group consisting of lung cancer cells, prostate cancer cells, gastric cancer cells, breast cancer cells, pancreatic cancer cells, colon cancer cells, and combinations thereof Cell culture method comprising a cell selected from the group consisting of muscle cells, stem cells, and combinations thereof. fuel; And, Fine bubbles formed in the fuel Containing, high efficiency mixed fuel. The method of claim 20, Wherein said fuel comprises one selected from the group consisting of fossil fuels, biofuels, and combinations thereof. The method of claim 20, The fine bubble is 10 ³ to 10 ^{18 is} formed per 1 mL of the fuel, high efficiency mixed fuel. The method of claim 20, The fine bubble will have a diameter of 1 nm to 1,000 ㎛, high efficiency mixed fuel. The method of claim 20, The high efficiency mixed fuel is a high efficiency mixed fuel having a viscosity of 0.4 MPa · s to 0.7 MPa · s. The method of claim 20, The high efficiency mixed fuel has a surface tension of 12 dyn / cm to 15 dyn / cm, high efficiency mixed fuel. A liquid tank into which liquid is injected; A gas supply line unit supplying gas to the liquid tank; And, Porous tube body installed inside the liquid tank A high efficiency mixed fuel manufacturing apparatus comprising a. The method of claim 26, Wherein the gas supply line unit is a high efficiency mixed fuel manufacturing apparatus comprising an inlet valve for injecting gas, a pressure gauge for measuring the pressure of the injected gas, and a supply pipe to which the injected gas is moved. The method of claim 27 The gas supply line unit further comprises a gas tank connected to the inlet valve, high efficiency mixed fuel manufacturing apparatus. The method of claim 27, The gas is supplied to the porous tube through the supply pipe, high efficiency mixed fuel manufacturing apparatus. The method of claim 26, The porous tube is a high efficiency mixed fuel manufacturing apparatus having a hole (hole) of 1 nm to 1 mm. The method of claim 29, The gas is converted into a fine bubble while passing through the porous tube, high efficiency mixed fuel manufacturing apparatus.

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