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WO2013004644A8 - Dispositif microfluidique doté de capteurs intégrés pour la culture de cellules - Google Patents

Dispositif microfluidique doté de capteurs intégrés pour la culture de cellules Download PDF

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Publication number
WO2013004644A8
WO2013004644A8 PCT/EP2012/062789 EP2012062789W WO2013004644A8 WO 2013004644 A8 WO2013004644 A8 WO 2013004644A8 EP 2012062789 W EP2012062789 W EP 2012062789W WO 2013004644 A8 WO2013004644 A8 WO 2013004644A8
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic device
chamber
cultivation
cells
integrated sensors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2012/062789
Other languages
English (en)
Other versions
WO2013004644A1 (fr
Inventor
Severine Le Gac
Van Den Albert Berg
Telma Cristina ESTEVES
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Twente Universiteit
Original Assignee
Twente Universiteit
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Twente Universiteit filed Critical Twente Universiteit
Publication of WO2013004644A1 publication Critical patent/WO2013004644A1/fr
Publication of WO2013004644A8 publication Critical patent/WO2013004644A8/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention porte sur un dispositif microfluidique (10) comprenant une chambre (12) destinée à contenir au moins un embryon (14), ladite chambre (12) délimitant un volume et comprenant une entrée (13) et une sortie (13'), dimensionnées pour permettre audit embryon (14) d'entrer et de sortir de ladite chambre (12) et ladite chambre (12) étant conçue pour contenir une solution de culture lorsque le ledit dispositif microfluidique (10) est en fonctionnement, ladite chambre (12) comprenant en outre au moins un capteur (33, 33') intégré dans ladite chambre (12) pour la mesure in situ d'au moins un paramètre choisi parmi les paramètres chimiques, physiques et biochimiques de ladite solution de culture lorsque ledit dispositif microfluidique (10) est en fonctionnement.
PCT/EP2012/062789 2011-07-01 2012-07-01 Dispositif microfluidique doté de capteurs intégrés pour la culture de cellules Ceased WO2013004644A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161503746P 2011-07-01 2011-07-01
US61/503,746 2011-07-01

Publications (2)

Publication Number Publication Date
WO2013004644A1 WO2013004644A1 (fr) 2013-01-10
WO2013004644A8 true WO2013004644A8 (fr) 2013-08-01

Family

ID=46456586

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/062789 Ceased WO2013004644A1 (fr) 2011-07-01 2012-07-01 Dispositif microfluidique doté de capteurs intégrés pour la culture de cellules

Country Status (1)

Country Link
WO (1) WO2013004644A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3017036B1 (fr) * 2013-07-05 2017-03-22 At Medical Ltd. Dispositif de culture d'un matériel biologique
EP3259342A4 (fr) * 2015-02-17 2018-10-17 Genea IP Holdings Pty Limited Procédé et appareil pour la culture dynamique d'un échantillon biologique
WO2020084324A1 (fr) * 2018-10-25 2020-04-30 Montanari, Bernard Capteur d'environnement pour incubateur ivf de mammifère
EP4144829A4 (fr) * 2020-04-30 2025-04-09 Xingyue Peng Procédé de construction d'une niche cellulaire artificielle microcyclique lente et dispositif correspondant
CN117660183B (zh) * 2024-01-31 2024-04-16 山东大学 胚胎微流控动态培养皿及其培养方法
WO2025179341A1 (fr) * 2024-02-28 2025-09-04 Cell Tech Holding Pty Ltd Dispositifs et procédés de micromanipulation

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
GB0505379D0 (en) * 2005-03-16 2005-04-20 Robio Systems Ltd Cellular entity maturation and transportation systems
CN101835886A (zh) * 2007-08-24 2010-09-15 智能生物系统有限公司 用于灌注的中等尺寸生物反应器平台
WO2009039433A1 (fr) * 2007-09-20 2009-03-26 Incept Biosystems Inc. Système de culture analytique microfluidique
US9201060B2 (en) * 2008-02-11 2015-12-01 Massachusetts Institute Of Technology Particle capture devices and methods of use thereof

Also Published As

Publication number Publication date
WO2013004644A1 (fr) 2013-01-10

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