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WO2013004280A1 - Séquences nucléotidiques mutées isolées qui codent pour une protéine modifiée oléate désaturase du tournesol, protéine modifiée, procédés et utilisations - Google Patents

Séquences nucléotidiques mutées isolées qui codent pour une protéine modifiée oléate désaturase du tournesol, protéine modifiée, procédés et utilisations Download PDF

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Publication number
WO2013004280A1
WO2013004280A1 PCT/EP2011/061164 EP2011061164W WO2013004280A1 WO 2013004280 A1 WO2013004280 A1 WO 2013004280A1 EP 2011061164 W EP2011061164 W EP 2011061164W WO 2013004280 A1 WO2013004280 A1 WO 2013004280A1
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ncimb
seq
sequence
protein
sunflower
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Inventor
Alberto Javier Leon
Andrés Daniel ZAMBELLI
Roberto Juan REID
Monica Mariel Morata
Marcos KASPAR
Enrique Martinez-Force
Rafael Garcés
Joaquin J. SALAS
Monica VENEGAS-CALERON
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Consejo Superior de Investigaciones Cientificas CSIC
Advanta International BV
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Consejo Superior de Investigaciones Cientificas CSIC
Advanta International BV
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Priority to PCT/EP2011/061164 priority Critical patent/WO2013004280A1/fr
Priority to ARP120102375A priority patent/AR087014A1/es
Publication of WO2013004280A1 publication Critical patent/WO2013004280A1/fr
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Priority to ARP240100392A priority patent/AR131904A2/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1464—Helianthus annuus [sunflower]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19006—DELTA12-fatty-acid desaturase (1.14.19.6), i.e. oleoyl-CoA DELTA12 desaturase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • ISOLATED MUTATED NUCLEOTIDE SEQUENCES THAT ENCODE A MODIFIED OLEATE DESTAURASE SUNFLOWER PROTEIN, MODIFIED PROTEIN,
  • the present invention relates to isolated mutated nucleotide sequences that encode a modified oleate
  • OLD desaturase sunflower protein
  • Oleate desaturase enzyme is involved in the enzymatic conversion of oleic acid to linoleic acid.
  • the microsomal OLD has been cloned and characterized by using the marker technology by T-DNA ("T-DNA tagging") (Okuley, et al., (1994) Plant Cell 6:147-158). Higher plants nucleotide sequences that encode microsomal OLD have been described in the document PCT W094/11516 of Lightner et al .
  • Sunflower is generally cultivated to obtain oils containing saturated fatty acids (palmitic and stearic) and unsaturated fatty acids (oleic and linoleic) .
  • Stearic acid content is always lower than 10% (Guston, FD et al . "The lipid handbook", Chapman and Hall 1986), usually between 3 and 7%.
  • unsaturated fatty acids there are two types of sunflower seeds: the normal sunflower which has a linoleic acid content between 50% and 70%
  • oilseeds focused on changing the composition of their fatty acids, for example by means of conventional breeding programs, mutagenesis or transgenesis .
  • Mutations are typically induced with extremely high doses of radiation and/or chemical mutagens (Gaul, H. Radiation Botany (1964) 4:155-232). High doses exceed the lethal dose of 50% (LD50), and generally the 90% lethal dose (LD90), which maximizes the percentage of possible
  • genotypes with low content of 18:1 into genotypes with a high content of 18:1 in their seed.
  • the accumulation of 18:1 fatty acids in seeds depends on two enzymatic reactions: the desaturation of 18:0 to 18:1 and the subsequent desaturation of 18:1 to 18:2.
  • the oleate desaturase enzyme (OLD) catalyzes the desaturation of oleic acid (18:1) to linoleic acid (18:2) (Ohlrogge and Browse (1995) The Plant Cell, 7:957-970, Somerville and Browse (1996) Trends Cell Biol 6:148-153; Schwartzbeck
  • Sunflower oil is naturally rich in linoleic acid (55-70%) and consequently low in oleic acid (20-25%) .
  • Traditional sunflower oil high in linoleic acid is considered a healthful vegetable oil that has a proper taste, and has been considered first quality in the world market due to the high percentage of polyunsaturated fatty acids. It is used as a dressing for salads, cooking oils, or for the production of margarine.
  • This oil should at least contain a concentration of oleic acid from 55 to 65% in relation to total fatty acids. The benefit of this oil is its high oxidative stability after the extraction process and the stability of the flavor in fried products.
  • Sunflower oil with a high oleic acid concentration does not require to be hydrogenated to improve its thermal stability, therefore does not include trans fatty acids.
  • Monounsaturated fatty acids display a good combination of low melting points, stability and viscosity (Noureddini et al . (1992) J Am Oil Chem Soc 12:1189-1191) .
  • the evolution of the oxidation of high oleic versus standard sunflower oils shows that a higher content of oleic acid gives a higher termooxidative resistance, with low peroxide value and polymer formation (Marmesat et al . (2009) Grasas y Aceites 60:155-160).
  • oils with high proportions of oleic acid would be much more resistant to oxidation than oils containing similar proportions of linoleic acid.
  • the invention thus relates to isolated nucleotide sequences encoding a modified oleate desaturase sunflower protein, wherein the nucleotide sequences comprise a point mutation.
  • nucleotide sequence encodes a modified oleate desaturase sunflower protein and comprises a point mutation.
  • the modified oleate desaturase sunflower protein comprises at least one of the following amino acid substitutions in the wild type
  • nucleotide sequence comprises one of the following amino acid sequences: SEQ ID No: 1; SEQ ID No: 2; SEQ ID No: 3; SEQ ID No: 4; SEQ ID No: 5; SEQ ID No: 6; SEQ ID No: 7; SEQ ID No: 8 or SEQ ID No: 9.
  • the nucleotide sequence is one of the following sequences: SEQ ID No: 10, SEQ ID No: 11; SEQ ID No: 12; SEQ ID No: 13; SEQ ID No: 14; SEQ ID No: 15; SEQ ID No: 16; SEQ ID No: 17 or SEQ ID No: 18; or sequences that are at least 90% homologous, wherein said sequences at least 90% homologous maintain the point
  • the percentage homology refers in particular to the percentage of identical residues in the sequence.
  • the invention further relates to a protein, in particular a sunflower protein, that has a reduced oleate desaturase activity, wherein said protein comprises at least one of the following amino acid substitutions with respect to the wild type sequence: a) Ser by Leu at position 131, b) Gly by Asp at position 103; c) Ser by Asn at position 135; d) Gly by Glu at position 144; e) Gly by Glu at position 226; f) Pro by Ser at position 253; g) Leu by Phe at
  • the protein has one of the following sequences: a) the sequence shown in SEQ ID No: 1; b) the sequence shown in SEQ ID No: 2; c) the sequence shown in SEQ ID No: 3; d) the sequence shown in SEQ ID No: 4; e) the sequence shown in SEQ ID No: 5; f) the sequence shown in SEQ ID No: 6; g) the sequence shown in SEQ ID No: 7; h) the sequence shown in SEQ ID No: 8; or SEQ ID No: 9.
  • the invention further relates to sunflower plants comprising a gene encoding an oleate desaturase protein having a point mutation modifying the amino acid sequence, and thereby affecting the activity of the oleate desaturase protein.
  • the nucleotide sequence of the gene comprises a point mutation with respect to the wild type sequence, as for example one or more of the following: a) G by A at position 308; b) C by T at position 392; c) G by A at position 404; d) G by A at position 431; e) G by A at position 677; f) C by T at position 757; g) C by T at position 799; h) C by T at position 823; o i) G by A at position 243.
  • the plant can produce seeds with an oleic content of between 80% and 95% with respect to the total percentage of the fatty acids of said seed.
  • the invention further relates to sunflower plants that produce an oleate desaturase protein that has a lower enzymatic activity as compared to the wildtype oleate desaturase protein without point mutations.
  • Such plants are obtainable by introgression of a mutation as found in a plant grown from seeds of which a representative sample was deposited under one of the accession numbers NCIMB 41735, NCIMB 41736, NCIMB 41737, NCIMB 41738, NCIMB 41739, NCIMB 41740, NCIMB 41741, NCIMB 41742 and NCIMB 41743 into a wildtype plant not carrying the mutation.
  • Introgression can for example take place by crossing a plant grown from seeds of which a representative sample was deposited under one of the accession numbers NCIMB 41735, NCIMB 41736, NCIMB 41737, NCIMB 41738, NCIMB 41739, NCIMB 41740, NCIMB 41741, NCIMB 41742 and NCIMB 41743 with another plant and selecting in the F2 for plants that produce a sunflower oil that has a high oleic acid content between 80 and 95% as compared to the total fatty acid content in the oil.
  • the invention further relates to plants producing an oleate desaturase protein having a combination of two of more of the following amino acid substitutions as compared to the wild type amino acid sequence: a) Gly by Asp at position 103, b) Ser by Leu at position 131; c) Ser by Asn at position 135; d) Gly by Glu at position 144; e) Gly by Glu at position 226; f) Pro by Ser at position 253; g) Leu by Phe at position 267; h) Pro by Ser at position 275, which plants are obtainable by introgression of the mutation as found in two or more of the plant grown from seeds of which a representative sample was deposited under one of the accession numbers NCIMB 41735, NCIMB 41736, NCIMB 41737, NCIMB 41738, NCIMB 41739, NCIMB 41740, NCIMB 41741, NCIMB 41742 and NCIMB 41743 into a wildtype sunflower or into a sunflower that already
  • Sunflower seeds comprising an oleate desaturase gene having a point mutation and that encodes a modified oleate desaturase protein are also provided.
  • the nucleotide sequence comprises at least one point mutation selected from: a) G by A at position 308; b) C by T at position 392; c) G by A at position 404; d) G by A at position 431; e) G by A at position 677; f) C by T at position 757; g) C by T at position 799; h) C by T at position 823; and i) G by A at position 243.
  • the seeds are seeds of which a representative sample was deposited at NCIMB and under one of the following accession numbers: NCIMB 41735, NCIMB 41736, NCIMB 41737, NCIMB 41738, NCIMB 41739, NCIMB 41740, NCIMB 41741, NCIMB 41742, NCIMB 41743.
  • the seed may have an oleic acid content of between 80% and 95% with respect to the total percentage of fatty acids of said seed.
  • At least one of the one or more point mutations present in homozygous state.
  • the genome of the plant or seed comprises more than one of the above listed point mutations, they are all present in homozygous state.
  • an oil is provided that is high in oleic acid, containing between 80% to 95% with respect to the total fatty acid percentage of the oil.
  • the oil can be obtained from seeds of which a representative sample was deposited under one of the accession numbers listed above.
  • the invention further relates to use of the sunflower seeds to obtain oil with high oleic acid content. Oil can suitably be obtained by extracting the seeds.
  • the invention also relates to progeny of the seeds and plants as described herein, wherein the progeny
  • a point mutation in the gene that encodes an oleate desaturase protein wherein said mutation leads to the synthesis of a modified oleate desaturase protein, in particular a oleate desaturase protein that has a reduced enzymatic activity as compared to a wildtype protein not carrying the point mutation.
  • step b) identifying and selecting at least one plant obtained in step b) comprising a nucleotide sequence having at least one point mutation and wherein said sequence encodes a protein that has modified oleate desaturase activity.
  • the point mutation comprises at least one
  • nucleotide substitution with respect to the wild type sequence such as : a) G by A at position 308; b) C by T at position 392; c) G by A at position 404; d) G by A at position 431; e) G by A at position 677; f) C by T at position 757; g) C by T at position 799; h) C by T at position 823; or i) G by A at position 243, wherein said sequence encodes a protein that has modified oleate
  • a method to identify sunflower plants or seeds with a high content of oleic acid which comprises:
  • the part of a plant is a seed.
  • the point mutation comprises at least one change in the nucleotide sequence encoding oleate desaturase, such as: a) G by A at position 308; b) C by T at position 392; c) G by A at position 404; d) G by A at position 431; e) G by A at position 677; f) C by T at position 757; g) C by T at position 799; h) C by T at position 823; or i) G by A at position 243, wherein said sequence encodes a protein that has modified oleate
  • the method to detect the presence of a point mutation in the nucleotide sequences of the invention may be any known technique, such as ASA (Soleimani et al . (2003) Plant Mol Biol Rep 21: 281-288), PAMSA (Gaudet et al . (2007) Plant Mol Biol Rep 25:1-9), SSCP (Germano and Klein (1999) Theor Appl Genet 99:37-49) or TaqMan® (Jones et al . (2008) Pest Management Science 64:12-15).
  • ASA Soleimani et al . (2003) Plant Mol Biol Rep 21: 281-288
  • PAMSA Gadet et al . (2007) Plant Mol Biol Rep 25:1-9
  • SSCP Germano and Klein (1999) Theor Appl Genet 99:37-49
  • TaqMan® Japanese Management Science 64:12-15.
  • the invention further relates to the use of the nucleotides sequence of the invention to prepare a probe to detect, in a sunflower plant, a molecular marker associated with the high oleic phenotype.
  • the nucleotide sequence can furthermore be used in a cisgenic or transgenic approach to make plants and seeds which produce a modified oleate desaturase resulting in a high oleic acid in the seed oil of the plant.
  • a procedure to obtain high oleic sunflower oil comprises extracting the oil from seeds of which a representative sample was deposited at NCIBM under accession number NCIMB 41735, NCIMB 41736, NCIMB 41737, NCIMB 41738, NCIMB 41739, NCIMB 41740, NCIMB 41741, NCIMB 41742 or NCIMB 41743.
  • Figure 1 shows the secondary structure of the native oleate desaturase protein (A) and point mutations (B) , a-h indicates the location of the different amino acid substitutions produced by the point mutations.
  • the oleate desaturase enzyme (OLD, EC 1.3.1.35) is also known as: oleic acid desaturase; linoleate synthase; oleoyl-CoA desaturase; oleoylphosphatidylcholine desaturase, oleoyl-PC desaturase, delta-12 oleate desaturase, FAD2.
  • the present invention is thus related to mutated nucleotides sequences that encode sunflower proteins having modified oleate desaturase activity, wherein the modified oleate desaturase encoded by said mutated polynucleotides has a lower enzymatic activity that leads the plant or parts of the plant, such as the seed, to contain an elevated percentage of oleic acid.
  • the changes or modifications in the amino acid sequence of the oleate desaturase protein may be one or more of the following substitutions :
  • the amino acid sequence of the modified oleate desaturase protein may be one of the sequences shown in SEQ ID Nos. 1 to 9. Any amino acid sequence of an oleate
  • desaturase protein comprising amino acid substitutions shown above are within the scope of the present invention.
  • the amino acid sequences shown as SEQ ID Nos. 1 to 9 can be modified also in other places than the sites of the substitutions shown here, being all of them within the scope of the present invention.
  • such reference is made by comparing the modified amino acid sequences with the sequence of the oleate desaturase from sunflower variety HA89, RHA266 and line 29002 (SEQ ID No:
  • oleate desaturase lines HA89, RHA266 and 29002 are the same and they all correspond to SEQ ID No: 19.
  • the point mutation can also generate a premature stop codon and then the nucleotide sequence will encode a truncated protein.
  • the truncated oleate desaturase protein is shown in SEQ ID No. 9.
  • the mutated nucleotide sequences have a point mutation that leads to the change of one amino acid for another in the encoded polypeptide. For example, the
  • nucleotide sequence can have any of the following changes: a) G by A at position 308;
  • the invention thus relates to any nucleotide substitution that leads to one or more of the following amino acid substitutions:
  • sequence of the mutated nucleotides of the invention is one of the
  • SEQ ID No: 10 SEQ ID No: 11, SEQ ID No: 12, SEQ ID No: 13, SEQ ID No: 14, SEQ ID No: 15, SEQ ID No: 16, SEQ ID No: 17 or SEQ ID No: 18.
  • the position of each of the point mutations indicated in the nucleotides sequence was made based on the nucleotides sequence that encodes the oleate desaturase of wild type of the sunflower variety HA89 (SEQ ID No: 20) .
  • mutated such as lines HA89, 29002 and RHA266 to obtain plants that produce seeds with high oleic acid content.
  • Selected mutations in the coding sequence of the sunflower oleate desaturase were point mutations that generated the substitution of an amino acid for another one in the amino acid sequence of the oleate desaturase.
  • the mutated seeds were deposited under the
  • Mutant plants and seeds of the invention can be obtained using different mutagenesis schemes.
  • a mutagenic agent is injected, for example EMS at a concentration between 5 and 15% to the heads of the plants.
  • the fatty acid profile of mutated M2 seeds and seed parentals were analyzed and only those were selected that showed a high content of oleic acid, for example over 80% with respect to the fatty acid content of the seed,
  • mutated plants and seeds were obtained by immersing the parental seeds in a mutagenic agent, such as EMS, at a concentration between 0.3 and 0.7%. Subsequently, the fatty acid profile of the M2 seeds was compared with the fatty acid profile of the
  • parental seeds and those that showed a high content of oleic acid for example over 80% with respect to the fatty acid content of seed, preferably about 90% or higher compared to the total fatty acid content of the seed were selected.
  • Table 1 shows the mutagenesis method used for each mutated line of the invention, the mutation obtained in the nucleotides sequence and the amino acid substitution in the sequence of the oleate desaturase enzyme.
  • P palmitic acid
  • S stearic acid
  • O oleic acid
  • L
  • Sunflower line 29074 presented a point mutation that exchanges the nucleotide C by T at position 392 of the coding region of the oleate desaturase (SEQ ID No: 11) . This change leads to the synthesis of a modified oleate
  • Sunflower line 29075 presented a point mutation that exchanges the nucleotide G by A at position 243 of the coding region of the oleate desaturase (SEQ ID No: 18)
  • the nucleotide exchange leads to the development of a stop codon that replaces tryptophan, generating a truncated modified oleate desaturase, and its amino acid sequence is shown in SEQ ID No: 9.
  • Sunflower line 29076 presented a point mutation exchanging nucleotide C by T at position 757 of the coding region of the oleate desaturase (SEQ ID NO: 15) . This change leads to the synthesis of an oleate desaturase modified wherein the amino acid proline is replaced by serine at position 253 (SEQ ID No. 6) corresponding to domain VIII, more particularly to the transmembrane helix (TM4) (f in
  • Sunflower line 29077 presented a point mutation exchanging nucleotide C by T at position 823 of the coding region of the oleate desaturase (SEQ ID No: 17) . This change leads to the synthesis of a modified oleate desaturase wherein the amino acid proline is replaced by serine at position 275 (SEQ ID No: 8) corresponding to domain IX formed by the C-terminal region (h in Figure 1) .
  • Sunflower line 29078 presented a point mutation exchanging the nucleotide G by A at position 404 of the coding region of the oleate desaturase (SEQ ID No: 12) . This change leads to the synthesis of a modified oleate
  • Sunflower line 29079 presented a point mutation exchanging the nucleotide G by A at position 431 of the coding region of the oleate desaturase (SEQ ID No: 13) . This change leads to the synthesis of a modified oleate
  • amino acid glycine is replaced by the amino acid glutamic acid at position 144 (SEQ ID No: 4) which corresponds to a site near the histidine domain II (d in Figure 1) .
  • Sunflower line 29082 presented a point mutation exchanging the nucleotide G by A at position 308 of the coding region of the oleate desaturase (SEQ ID No: 10) . This change leads to the synthesis of a modified oleate
  • amino acid glycine is replaced by the amino acid aspartic acid at position 103 (SEQ ID No. 1) which corresponds to a site near the histidine domain I (a in Figure 1) .
  • Sunflower line 29081 presented a point mutation exchanging nucleotide C by T at position 799 of the coding region of the oleate desaturase (SEQ ID No: 16) . This change leads to the synthesis of a modified oleate desaturase wherein the amino acid leucine is substituted by the amino acid phenylalanine at position 267 (SEQ ID No. 7) located in domain IX (g in Figure 1) .
  • Sunflower line 39096 presented a point mutation exchanging the nucleotide G by A at position 677 of the coding region of the oleate desaturase (SEQ ID No. 14) . This change leads to the synthesis of a modified oleate
  • the point mutations of the invention can be introduced into an agronomically desirable background. This can be done analogous to prior art describing how different point mutations associated to agronomical traits of interest were introgressed into elite material. For instance, herbicide resistant trait was introgressed into elite inbred lines of sunflower by
  • RHA 447 is an F7-derived F8 restorer sunflower line selected from the cross RHA 377/RHA 348.
  • RHA 377 PI 560145
  • RHA 348 PI 509058
  • a high-oleic oilseed germplasm are restorer lines released by USDA-ARS and the North Dakota Agricultural Experiment Station in 1990 and 1986,
  • the pedigree breeding method was used to develop RHA 447. Analyses for oleic acid concentration were conducted on seed harvested from F3 to F7 plants by gas chromatography (Miller et al . (2006) Crop Sci 46:484-485).
  • the oil obtained from any of the sunflower seeds of the invention has an oleic acid content higher than 80% with respect to the total fatty acid content of seed, preferably greater than 85% and more preferably greater than 90%.
  • sequences of the disclosed nucleic acids can be used as molecular markers or probes provided that part of the sequence
  • the exchange of C by T at nucleotide position 392 (corresponding to codon 131 that encodes for Leucine) shown as SEQ ID No: 11 can be used as a molecular marker to detect, or select sunflower plants with high oleic acid content, using any known techniques such as ASA (Soleimani et al . (2003) Plant Mol Biol Rep 21: 281-288), PAMSA (Gaudet et al . (2007) Plant Mol Biol Rep 25:1-9) SSCP (Germano and Klein (1999) Theor Appl Genet 99:37-49) or TaqMan® (Jones et al . (2008) Pest Management Science 64:12-15).
  • ASA Soleimani et al . (2003) Plant Mol Biol Rep 21: 281-288
  • PAMSA Gaudet et al . (2007) Plant Mol Biol Rep 25:1-9
  • SSCP Germano and Klein (1999) Theor Appl Genet 99:37-49
  • TaqMan® Japanese Management Science
  • a portion of the sequence shown as SEQ ID No. 12 which comprises the exchange of G by A at nucleotide position 404 can be used as a molecular marker to detect, or select sunflower plants with high oleic acid content, using any known techniques described above.
  • a portion of the sequence shown as SEQ ID No. 13 which includes the exchange of G by A at nucleotide position 431 can be used as a molecular marker to detect, or select sunflower plants with high oleic acid content, using any known techniques described above.
  • a portion of the sequence shown as SEQ ID NO: 14 comprising the exchange of G by A at nucleotide position 677 can be used as a molecular marker to detect, or select sunflower plants with high oleic acid content, using any known techniques described above.
  • a portion of the sequence shown as SEQ ID NO: 15 comprising the exchange of T by C at nucleotide position 757 can be used as a molecular marker to detect, or select sunflower plants with high oleic acid content, using any known techniques described above.
  • a portion of the sequence shown as SEQ ID NO: 16 comprising the exchange of T by C at nucleotide position 799 can be used as a molecular marker to detect, or select sunflower plants with high oleic acid content, using any known techniques described above.
  • a portion of the sequence shown as SEQ ID No. 17 which includes the exchange of C by T at nucleotide position 823 (corresponding to codon 275 that encodes serine) can be used as a molecular marker to detect, or select sunflower plants with high oleic acid content, using any known techniques described above.
  • a portion of the sequence shown as SEQ ID No. 18 which includes the exchange of G by A at nucleotide position 243 (corresponding to codon 81 that encodes Stop) can be used as a molecular marker to detect, or select sunflower plants with high oleic acid content, using any know techniques described above. It is apparent to those skilled in the art that shown mutations can be useful to identify sunflower plants with high oleic acid content in any scheme of plant breeding and using any known technique.
  • the mutated nucleotide sequences can also be used to be introduced through an appropriate vector to a plant, where the genetically modified plant obtained is a plant with a high oleic acid content phenotype.
  • batch CA04-3 and 40 rows identified as batch CA04-3 and 40 rows as a batch CA04-1501. Each row was 6 meters long.
  • the EMS is a mutagenic agent that induces transitions G/C to A/T (Jander et al . (2003. Plant Physiol. 131:139-146).
  • Ml self-pollinated seeds each M0 plant was bagged before flowering. The flower heads of the plants of each EMS treatment were harvested, threshed and stored.
  • Ml seeds HA89-10% EMS (seeds mutagenized with 10% EMS) were sown in batches ca05, ca05-6077 and CA05- 6079. In this way, a plot of 1 ha with origin CA04-3 was obtained and a plot of 0.6 ha of origin CA04-1501 was obtained. An additional lot of 0.6 ha was planted with HA89 mutated with 15% of EMS (originally CA04-1501) known as Lot ca05-6080.
  • a total of 12,000 Ml plants from each batch was bagged for self-pollination and the flower heads of the rows were harvested and threshed individually.
  • M2 seeds were taken from each plant and analyzed by infrared spectroscopy (NIR) according to the protocol described in Fassio and Cozzolino, Industrial Crops and Products (2004) 20:321-329.
  • NIR infrared spectroscopy
  • composition of the selected seeds by NIR was analyzed by gas chromatography (GC) , evaluating 30 individual seeds of each M2 flower head, according to the protocol described in
  • the mutant plant CA06- 497-827 showed a phenotype of high-oleic acid content. It was identified and referred to as 29081.
  • RHA266 line was sown in Balcarce (wholesome Aires, Argentina) in the season 2004/5 and 103 rows were identified under the lot number CA04-2. Each row was six meters long.
  • WO2008/071715 Each M0 plant was bagged before flowering in order to produce the Ml self-pollinated seeds. The flower heads of the plants of each EMS treatment were harvested, threshed and stored. In the next field planting season, Ml seeds RHA266-15% EMS were planted in one hectare on December 1, 2005 under the lot number CA05-6073. A total of 12,000 Ml plants were bagged and the 10,400 flower heads resulting that reached maturity were harvested and threshed
  • Sunflower seeds are cut by the sagittal axis and placed in a 2ml glass vial containing 0.25 ml of methylation solution consisting of methanol, toluene, dimethoxypropane and sulfuric acid in the ratio 66:28:4:2.
  • the seeds were covered and incubated for one hour at 80 °C. They were allowed to cool at room temperature and then 1 ml of heptane was added (Garces and Mancha (2003) Anal Biochem 317:247- 254) .
  • the methyl esters of present fatty acids in the superior phase (heptane) were separated on a gas
  • tissue samples were taken from each high-oleic mutant. Genomic DNA was isolated and diluted to a stock concentration of 100 ng/ ⁇ . The coding sequence of the oleate desaturase of the high oleic mutants and the coding sequence of the oleate desaturase of line HA89 (wild type) were amplified in two overlapping segments. The specific primers used for each amplificon were: ler amplicon (705 bp)
  • OLD1-F2 GAAAAGTCTGGTCAAACAGTCAACAT SEQ ID No: 21
  • OLD1-R2 CCGATGTCGGACATGACTATC SEQ ID No: 22
  • PCR polymerase chain reaction
  • IX buffer Invitrogen
  • 0.2 mm dNTPs GE Healthcare
  • 2.5 mm MgC ⁇ 2 Invitrogen
  • 0.2 ⁇ of each primer 0.5 ⁇ 1 of Platinum Taq DNA polymerase (5 ⁇ / ⁇ 1) (Invitrogen) and lOOng of genomic DNA.
  • the PCR reaction was carried out on a GeneAmp PCR
  • Terminator kit v3.1 Cycle Sequencing (Applied Biosystems) following the manufacturer's instructions.
  • the files of the sequencing of the oleate desaturase obtained for each amplicon were assembled using the program Vector NTI Suite-Contig Express, version 7.0 (Informax) .
  • the resulting DNA sequences of the oleate desaturase were aligned with the sequences of line HA89 (GenBank Accession Number AY802989) .

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Abstract

L'invention concerne des séquences nucléotidiques isolées, comprenant une mutation ponctuelle et les séquences codant pour une protéine modifiée oléate désaturase du tournesol. La protéine modifiée oléate désaturase du tournesol comprend au moins une des substitutions d'acides aminés suivantes : a) Gly par Asp à la position 103, b) Ser par Leu à la position 131, c) Ser par Asn à la position 135, d) Gly par Glu à la position 144, e) Gly par Glu à la position 226, f) Pro par Ser à la position 253, g) Leu par Phe à la position 267, h) Pro par Ser à la position 275 ou est une oléate désaturase tronquée.
PCT/EP2011/061164 2011-07-01 2011-07-01 Séquences nucléotidiques mutées isolées qui codent pour une protéine modifiée oléate désaturase du tournesol, protéine modifiée, procédés et utilisations Ceased WO2013004280A1 (fr)

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PCT/EP2011/061164 WO2013004280A1 (fr) 2011-07-01 2011-07-01 Séquences nucléotidiques mutées isolées qui codent pour une protéine modifiée oléate désaturase du tournesol, protéine modifiée, procédés et utilisations
ARP120102375A AR087014A1 (es) 2011-07-01 2012-06-29 Secuencias de nucleotidos mutadas aisladas que codifican una proteina oleato desaturasa modificada de girasol, proteina modificada, metodos y usos
ARP240100392A AR131904A2 (es) 2011-07-01 2024-02-21 Secuencias de nucleótidos mutadas aisladas que codifican una proteína oleato desaturasa modificada de girasol, proteína modificada, métodos y usos

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021014010A1 (fr) 2019-07-24 2021-01-28 Soltis Tournesol à teneur élevée en acide oléique et procédé d'obtention
WO2024094771A1 (fr) 2022-11-02 2024-05-10 KWS SAAT SE & Co. KGaA Mutants du tournesol, à teneur en acide oléique, et procédés de détection

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994011516A1 (fr) 1992-11-17 1994-05-26 E.I. Du Pont De Nemours And Company Genes pour des desaturases d'acides gras en position delta-12 microsomales et enzymes apparentees provenant de plantes
US6342658B1 (en) * 1995-12-14 2002-01-29 Cargill, Incorporated Fatty acid desaturases and mutant sequences thereof
US20040083502A1 (en) 2001-02-16 2004-04-29 Enrique Martinez-Force Plant, seeds and oil with increased saturated triacylglycerols content and oil having a high steric acid content
WO2005106022A2 (fr) 2004-04-29 2005-11-10 Institut National De La Recherche Agronomique Procede destine a selectionner des genotypes de tournesol a teneur elevee en acide oleique dans l'huile de graine
WO2006024351A1 (fr) 2004-07-30 2006-03-09 Basf Agrochemical Products B.V. Plants de tournesol resistant aux herbicides, polynucleotides codant pour des proteines a large sous-unite d'acetohydroxy acide synthase resistant aux herbicides, et methodes d'utilisation
WO2007038738A2 (fr) * 2005-09-28 2007-04-05 Dow Agrosciences Llc Tournesol a forte teneur en acide oleique resistant a l'imidazolinone
WO2008071715A1 (fr) 2006-12-12 2008-06-19 Basf Agrochemical Products B.V. Plants de tournesol résistants aux herbicides et leurs procédés d'utilisation
US20090158452A1 (en) * 2001-12-04 2009-06-18 Johnson Richard G Transgenic plants with enhanced agronomic traits

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994011516A1 (fr) 1992-11-17 1994-05-26 E.I. Du Pont De Nemours And Company Genes pour des desaturases d'acides gras en position delta-12 microsomales et enzymes apparentees provenant de plantes
US6342658B1 (en) * 1995-12-14 2002-01-29 Cargill, Incorporated Fatty acid desaturases and mutant sequences thereof
US20040083502A1 (en) 2001-02-16 2004-04-29 Enrique Martinez-Force Plant, seeds and oil with increased saturated triacylglycerols content and oil having a high steric acid content
US20090158452A1 (en) * 2001-12-04 2009-06-18 Johnson Richard G Transgenic plants with enhanced agronomic traits
WO2005106022A2 (fr) 2004-04-29 2005-11-10 Institut National De La Recherche Agronomique Procede destine a selectionner des genotypes de tournesol a teneur elevee en acide oleique dans l'huile de graine
WO2006024351A1 (fr) 2004-07-30 2006-03-09 Basf Agrochemical Products B.V. Plants de tournesol resistant aux herbicides, polynucleotides codant pour des proteines a large sous-unite d'acetohydroxy acide synthase resistant aux herbicides, et methodes d'utilisation
WO2007038738A2 (fr) * 2005-09-28 2007-04-05 Dow Agrosciences Llc Tournesol a forte teneur en acide oleique resistant a l'imidazolinone
WO2008071715A1 (fr) 2006-12-12 2008-06-19 Basf Agrochemical Products B.V. Plants de tournesol résistants aux herbicides et leurs procédés d'utilisation

Non-Patent Citations (50)

* Cited by examiner, † Cited by third party
Title
AL-KHATIB, MILLER, CROP SCI, vol. 40, 2000, pages 869 - 870
DATABASE EMBL [Online] 12 August 1997 (1997-08-12), HNGTRAKUL V ET AL: "Helianthus annuus delta-12 oleate desaturase mRNA, complete cds", XP002297878, retrieved from EBI Database accession no. U91341 *
DATABASE NPGS [Online] ARS-GRIN; 1 January 2006 (2006-01-01), XP002661803, Database accession no. PI 639168 *
DATABASE NPGS [Online] ARS-GRIN; 1 January 2006 (2006-01-01), XP002662795, Database accession no. PI 639169 *
DATABASE NPGS [Online] ARS-GRIN; 1 January 2006 (2006-01-01), XP002662796, Database accession no. PI 639167 *
DATABASE NPGS [Online] ARS-GRIN; 1 January 2006 (2006-01-01), XP002662797, Database accession no. PI 639170 *
DATABASE NPGS [Online] ARS-GRIN; 1 January 2006 (2006-01-01), XP002662804, Database accession no. PI 639165 *
DATABASE NPGS [Online] ARS-GRIN; 1 May 2004 (2004-05-01), XP002662798, Database accession no. PI 633746 *
DATABASE NPGS [Online] ARS-GRIN; 1 May 2004 (2004-05-01), XP002662799, Database accession no. PI 633747 *
DATABASE NPGS [Online] ARS-GRIN; 1 May 2004 (2004-05-01), XP002662800, Database accession no. PI 633748 *
DATABASE NPGS [Online] ARS-GRIN; 1 May 2004 (2004-05-01), XP002662801, Database accession no. PI 633744 *
DATABASE NPGS [Online] ARS-GRIN; 1 November 1987 (1987-11-01), XP002662807, Database accession no. PI 509065 *
DATABASE NPGS [Online] ARS-GRIN; 1 November 1987 (1987-11-01), XP002662808, Database accession no. PI 509064 *
DATABASE NPGS [Online] ARS-GRIN; 1 November 2006 (2006-11-01), XP002662802, Database accession no. PI 642775 *
DATABASE NPGS [Online] ARS-GRIN; 1 November 2006 (2006-11-01), XP002662803, Database accession no. PI 642777 *
DATABASE NPGS [Online] ARS-GRIN; 1 November 2006 (2006-11-01), XP002662805, Database accession no. PI 642774 *
DATABASE NPGS [Online] ARS-GRIN; 1 November 2006 (2006-11-01), XP002662806, Database accession no. PI 642772 *
FASSIO, COZZOLINO, INDUSTRIAL CROPS AND PRODUCTS, vol. 20, 2004, pages 321 - 329
FERNANDEZ-MARTINEZ J. M. ETAL: "Sunflower mutant containing high levels of palmitic acid in high oleicbackground", vol. 97, 1 January 1997 (1997-01-01), pages 113 - 116, XP055019185, Retrieved from the Internet <URL:http://www.springerlink.com/content/gq23k6w6q217p365/fulltext.pdf> [retrieved on 20120213] *
FICK, MILLER: "Sunflower Technology and Production", 1997, AMERICAN SOCIETY OF AGRONOMY, MADISON, article "Sunflower breeding", pages: 395 - 439
GARCES R, GARCIA J, MANCHA M: "Lipid characterization in seeds of a high oleic acid sunflower mutant", PHYTOCHEMISTRY, vol. 28, 1989, pages 2597 - 2600, XP026633565, DOI: doi:10.1016/S0031-9422(00)98047-6
GARCES R, MANCHA M: "In vitro oleate desaturase in sunflower seeds developping", PHYTOCHEMISTRY, vol. 30, 1991, pages 2127 - 2130, XP026631299, DOI: doi:10.1016/0031-9422(91)83599-G
GARCES, MANCHA, ANAL BIOCHEM, vol. 317, 2003, pages 247 - 254
GARCÉS, MANCHA, ANAL BIOCHEM, vol. 317, 2003, pages 247 - 254
GAUDET ET AL., PLANT MOL BIOL REP, vol. 25, 2007, pages 1 - 9
GAUL, H., RADIATION BOTANY, vol. 4, 1964, pages 155 - 232
GERMANO, KLEIN, THEOR APPL GENET, vol. 99, 1999, pages 37 - 49
GUNSTONE: "The Chemistry of Fats and Oils", 2004, BLACKWELL PUBLISHING, pages: 150 - 168
GUSTON, FD ET AL.: "The lipid handbook", 1986, CHAPMAN AND HALL
HONGTRAKUL ET AL., CROP SCI, vol. 38, 1998, pages 1245 - 1249
JANDER ET AL., PLANT PHYSIOL., vol. 131, 2003, pages 139 - 146
JONES ET AL., PEST MANAGEMENT SCIENCE, vol. 64, 2008, pages 12 - 15
KABBAJ A ET AL: "EXPRESSION D'UNE STEARATE ET D'UNE OLEATE DESATURASES CHEZ LE TOURNESOL NORMAL ET A HAUTE TENEUR EN ACIDE OLEIQUE, CLONAGE DE FRAGMENTS GENOMIQUES ET VARIABILITE CHEZ QUELQUES HELIANTHUS//EXPRESSION OF A STEARATE AND OLEATE DESATURASE IN NORMAL AND IN HI", O.C.L. OLEAGINEUX CORPS GRAS LIPIDES, EDITIONS JOHN LIBBEY EUROTEXT, MONTROUGE, FR, vol. 3, no. 6, 1 November 1996 (1996-11-01), pages 452 - 458, XP008035426, ISSN: 1258-8210 *
KNOWLES, PF: "Recent advances in oil crops breeding", AOCS PROCEEDINGS, 1988
LACOMBE ET AL., LIFE SCI, vol. 324, 2001, pages 839 - 845
MARMESAT ET AL., GRASAS Y ACEITES, vol. 60, 2009, pages 155 - 160
MILLER ET AL., CROP SCI, vol. 46, 2006, pages 484 - 485
MILLER, AL-KHATIB, CROP SCI, vol. 42, 2002, pages 988 - 989
MILLER, AL-KHATIB, CROP SCI, vol. 44, 2004, pages 1037 - 1038
NOUREDDINI ET AL., J AM OIL CHEM SOC, vol. 12, 1992, pages 1189 - 1191
OHLROGGE, BROWSE, THE PLANT CELL, vol. 7, 1995, pages 957 - 970
OKULEY ET AL., PLANT CELL, vol. 6, 1994, pages 147 - 158
SCHNEITER, MILLER, CROP SCI, vol. 21, 1981, pages 901 - 903
SCHWARTZBECK, PHYTOCHEMISTRY, vol. 57, 2001, pages 643 - 652
SKORIC, D. ET AL: "DOSTIGNU]A I DALJI PRAVCI U OPLEMENJIVANJU SUNCOKRETAI IZBOR HIBRIDA ZA SETVU U 2002. GODINI", no. 36, 1 January 2002 (2002-01-01), XP055019086, Retrieved from the Internet <URL:http://scindeks-clanci.nb.rs/data/pdf/0351-4781/2002/0351-47810236147S.pdf> [retrieved on 20120210] *
SKORIC, SUNFLOWER BREEDING, vol. 25, 1988, pages 3 - 90
SOLDATOV, KI: "Chemical mutagenesis in sunflower breeding", PROC. 7TH INTERNATIONAL SUNFLOWER CONFERENCE, 1976, pages 352 - 357
SOLEIMANI ET AL., PLANT MOL BIOL REP, vol. 21, 2003, pages 281 - 288
SOMERVILLE, BROWSE, TRENDS CELL BIOL, vol. 6, 1996, pages 148 - 153
VARÈS D. ET AL: "INHERITANCE OF OLEIC ACID CONTENT OF F1 SEEDIN A COMPLETE DIALLEL CROSS BETWEEN SEVENSUNFLOWER LINES", vol. 25, no. 36, 1 July 2002 (2002-07-01), pages 105 - 112, XP055019511, Retrieved from the Internet <URL:http://www.doiserbia.nb.rs/img/doi/1018-1806/2002/1018-18060236105V.pdf> [retrieved on 20120215] *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021014010A1 (fr) 2019-07-24 2021-01-28 Soltis Tournesol à teneur élevée en acide oléique et procédé d'obtention
FR3099178A1 (fr) 2019-07-24 2021-01-29 Soltis Tournesol à teneur élevée en acide oléique et procédé d’obtention
WO2024094771A1 (fr) 2022-11-02 2024-05-10 KWS SAAT SE & Co. KGaA Mutants du tournesol, à teneur en acide oléique, et procédés de détection

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