WO2012139751A1 - Antimicrobial active substance preparation - Google Patents

Abstract

The present invention relates to an antimicrobial active substance preparation which comprises the following components: a) a hydrogen peroxide source selected from the group consisting of H₂O₂, sodium perborate and sodium percarbonate, b) an acyloxybenzoic acid (OBA) with the general chemical formula: in which R is a linear or branched, saturated alkyl group having 6 to 22 carbon atoms, a linear or branched, mono- or polyunsaturated alkenyl group having 6 to 22 carbon atoms or an aryl group having 6 to 22 carbon atoms, and c) optionally tetraacetylethylenediamine (TAED). The invention is particularly suitable for eliminating microorganisms, in sterilants, disinfectants, impregnating agents or preservatives, detergents or cleaning agents at low temperatures, or in coolants or cooling lubricants, and in the field of water purification and in the pharmaceutical, food, brewing, medical-technical, colour, timber, textile, cosmetics, leather, tobacco, fur, rope, paper, cellulose, plastics, fuel, oil, rubber or machine industry.

Description

 Antimicrobial agent preparation

In the private household, the washing habits in terms of

Temperature changed significantly in the past few years. Starting with the cooking linen, which used to be washed predominantly at a water temperature of 95 ° C, meanwhile, a water temperature of only 60 ° C has prevailed. The trend is currently even 40 ° C and possibly even lower temperatures. Although this has the advantage that energy can be saved in significant amounts, but by lowering the temperature there are serious disadvantages in terms of reducing the washing performance and hygiene. Bacteria, fungi and viruses can easily survive the washing process and remain on the laundry fiber or even transfer to other laundry items. Next it is possible that remaining

 Increase germs / fungal spores in the washing machine and thus can lead to putrid and unpleasant musty odor.

The lack of effect of the temperature can usually be compensated in part by adding bleaching systems. Depending on the bleaching system but there

Differences in the mode of action on different germs, such as bacteria, yeasts,

Mold fungi, bacteriophages (see Betz et al., Surfactants Surf., Det. 38 (2001)

4). Common bleaching systems such as PAP (phthalimidoperoxocaproic acid) or NOBS

(Nonanoyloxobenzoic acid sulfonate sodium salt) or TAED

(Tetraacetylethylenediamine) have a microbicidal potential, which is derived from

 Bleach activator itself, from the concentrations, the pH value, and the

Temperature is significantly influenced.

The hygiene effect of Betz et al. examined activators show clear differences. The Percarbonate plus TAED system, which is used predominantly in heavy-duty detergents on the European market, is only significantly reducing the bacterial count of Gram-positive bacteria (Enterococcus feacium) a period of 40 minutes washing time at 30 ° C. The same weak result is obtained in other bacteria (Staphylococcus aureus).

The effect of the NOBS system on Gram-positive and Gram-negative bacteria is described in more detail in US Pat. No. 6,551,975.

In Household and Personal Care Today, n2 / 2010, Hall et al.

described that copper-based homogeneous catalysts in detergents can be used successfully for germ reduction. However, these are expensive to produce, and the copper has the additional disadvantage that ecological and toxicological considerations against its use in

Detergents can speak.

The press release of the Hohenstein Institute in Bönnigheim dated 16 March 2010 describes that there is no solution for the

Problem is that at washing temperatures below 60 ° C athlete's foot spores

(Trychophyton rubrum), which are among the dermatophytes and are known to kill with relatively high reliability, a specific fungal infection of the skin, dermatophytosis, are killed by detergent additives and rendered harmless. If contaminated clothing is washed at 30 ° C, there is an acute risk that these germs will remain in the washing machine or be transferred to other items of laundry and thus infect other people. Specifically, the spores of the fungi are very stable and can remain infective over the period of months. Transmission takes place through contact infection.

Object of the present invention, therefore, was an antimicrobial

Provide active agent preparation available that can be used, for example, when cleaning laundry at low temperatures in the range of 20 to 50 ° C and with which it is possible despite the low temperature, specifically yeasts and fungi, especially athlete's foot and athlete's foot

(Trychophyton rubrum) harmless and thus the risk of transmission this pathogen on other laundry items or the formation of odor on the

To prevent laundry fibers or inside the washing machine.

This object is achieved by an antimicrobial

Solvent preparation which is effective in particular against mycelium and spores at pH> 7, comprising: a hydrogen peroxide source selected from the group consisting of H2O2, sodium perborate and sodium percarbonate, an acyloxybenzoic acid (OBA) having the general chemical formula

in der R eine lineare oder verzweigte, gesättigte Alkylgruppe mit 6 bis 22 C-Atomen, eine lineare oder verzweigte, ein- oder mehrfach ungesättigte Alkenylgruppe mit 6 bis 22 C-Atomen oder eine Arylgruppe mit 6 bis 22 C-Atomen bedeutet, und c) und gegebenenfalls Tetraacetylethylendiamin (TAED), gegebenenfalls in Kombination mit einem Bindemittel.

in which R is a linear or branched, saturated alkyl group having 6 to 22 C atoms, a linear or branched, mono- or polyunsaturated alkenyl group having 6 to 22 C atoms or an aryl group having 6 to 22 C atoms, and c and optionally tetraacetylethylenediamine (TAED), optionally in combination with a binder.

Preferred radicals R in the acyloxybenzoic acid (OBA) are linear or branched saturated alkyl groups having 7 to 18 C atoms or linear or branched unsaturated alkylene groups having 7 to 18 C atoms or a

Aryl group with 6 to 10 carbon atoms.

Very particularly preferred radicals R in the acyloxybenzoic acid (OBA) are linear saturated alkyl groups having 8 to 12 C atoms or linear unsaturated alkylene groups having 8 to 12 C atoms or an aryl group having 6 C atoms. Most preferably, the radical R in the acyloxybenzoic acid (OBA) is a linear saturated alkyl group having 10 C atoms, this being

Compound is then decanoyloxybenzoic acid (DOBA). The ratio of OBA to hydrogen peroxide source is in the

active agent preparation according to the invention in the range of 1: 1 to 1: 15, measured in molar proportions, preferably from 1: 2 to 1: 5, particularly preferably 1: 3. The mixing ratio of OBA to TAED in the inventive

 Active agent preparation can vary and is preferably in the range from 10: 1 to 1:10 parts by weight, preferably in the range from 2: 1 to 1: 2.

The active content, which is the sum of OBA plus TAED, expressed in wt .-%, this mixture is from 50 to 99 wt .-%. The remainder, in the case of a co-granule, may be a binder of the following group: waxes, silicones, fatty acids, fatty alcohols, soaps, anionic surfactants, nonionic surfactants, cationic surfactants, anionic and cationic polymers and polyalkylene glycols. Particularly preferred examples are: C8-C31 fatty acids such as lauric, myristic stearic, Ce-C ₃ i fatty alcohols, polyethylene glycols having a molecular weight from 1000 to 50,000 g / mol, fatty alcohol with 1-00 moles of ethylene glycol units in the Molecule, alkanesulfonates,

Alkylbenzenesulfonates, alpha-olefinsulfonates, alkyl sulfates, alkyl ether sulfates with C 8 -C 31 hydrocarbon radicals, polymers such as polyvinyl alcohols, waxes such as montan waxes, paraffin waxes, ester waxes, polyolefin waxes, silicones.

OBA and TAED can be used according to the invention in powder form or in granulated form. In a preferred embodiment of the invention, OBA and TAED are presented as unitary granules.

The antimicrobial agent preparation according to the invention is biodegradable and non-sensitizing. Surprisingly, it has been found that the antimicrobial active agent preparation according to the invention is able to significantly reduce the spore and germ count of microorganisms, yeasts and fungi, including, particularly surprisingly, that of the particularly unpleasant dermatophytes. The microbicidal effect of the active agent preparation according to the invention was surprisingly found at low temperatures of <60 ° C, particularly surprisingly even at room temperature.

The invention relates to the use of the antimicrobial active agent preparation in a powder detergent or in liquid detergents or in detergents of pasty form. The use leads to the release of peracids having at least 6 and at most 22 C atoms, preferably at least 8 and a maximum of 12 C atoms, more preferably of 10 C atoms.

These peracids significantly reduce the activity of microorganisms, yeasts and fungi, including especially dermatophytes, preferably Trichophyton and most preferably Trichophyton rubrum. When used in combination with TAED, a synergistic effect is surprisingly observed in this regard.

Chemical reaction of OBA with H ₂ O ₂ splits off para-hydroxybenzoic acid, which is known to have preservative properties. This has a particularly beneficial effect on the hygiene effect of

Agent preparation from. When using activators based on phenolsulfonates, this is not the case.

In a further embodiment, therefore, the inventive

Use for the removal of microorganisms, in sterilization,

Disinfecting, impregnating or preserving agents, washing or

Detergents, or in cooling or cooling lubricants (technical

Application solutions) as well as in the field of water purification and

Water treatment and the pharmaceutical, food, brewery,

Medical, paint, wood, textile, cosmetics, leather, tobacco, fur, rope, Paper, pulp, plastic, fuel, oil, rubber or

Machinery industry. In a further preferred embodiment, the use according to the invention for controlling the number of microorganisms thus takes place in medical devices, instruments and apparatus, in particular in catheters and endoscopes, in which the adhesion of germs can, as is known, have particularly unpleasant consequences.

microorganisms

 Microorganisms are fungi in particular. This includes in particular spores, which serve as propagation structures in mushrooms. In a preferred embodiment, microorganisms are keratinophilic fungi, such as dermatophytes of the genus Microsporum,

Trichophyton and Epidermophyton. Next are under a preferred one

Embodiment fungi to understand the strain of Ascomycota

(Ascomycetes) belonging to the class Eurotiomycetes, the order Onygenales and the family Arthrodermataceae. This represents a particularly preferred embodiment of the genus Trichophyton and from the species Trichophyton rubrum. The use of the active agent preparation according to the invention reduces the accumulation of bacteria, in particular the accumulation of Gram-negative and Gram-positive bacteria, in particular the adhesion of pathogenic bacteria selected from Propionibacterium acnes, Stapylococcus aureus, Streptococcus group A (beta-hemolytic S.), S. pyogenes, Corynebacterium spp. (especially C. tenuis, C. diphtheriae, C. minutissimum), Micrococcus spp. (especially M. sedentarius), Bacillus anthracis, Neisseria meningitidis, N. gonorrhoeae, Pseudomonas aeruginosa, P. pseudomallei, Borrelia burgdorferi, Treponema pallidum, Mycobacterium tuberculosis,

Mycobacterium spp., Escherichia coli and Streptococcus spec. (especially S. gordonii, S. mutans), Actinomyces spec. (especially A. naeslundii),

Salmonella spec, Actinobacteria (especially Brachybacterium spec), alpha-Proteobacteria (especially Agrobacterium spec), beta-Proteobacteria (especially Nitrosomonas spec), Aquabacterium spec, Hydrogenophaga, gamma-proteobacteria, Stenotrophomonas sp., Xanthomonas spec.

(campestris), Neisseria spec., Haemophilus spec. and all microorganisms described by Paster et al. (J. Bac. 183 (2001) 12, 3770-3783). The use of the antimicrobial agent preparation also reduces the accumulation of human pathogenic fungi. These are for

Examples include the human pathogenic species of the fungi from the classes Ascomycota, Basidomycota, Deuteromycota and Zygomycota, in particular all species of the genera Aspergillus, Penicillium, Cladosporium and Mucor and Stachybotrys, Phoma, Alternaria, Aureobasidium, Ulocladium, Epicoccum,

 Stemphyllium, Paecilomyces, Trichoderma, Scopulariopsis, Wallemia, Botrytis, Verticillium and Chaetonium as well as the human pathogenic forms of Candida.

Enrichment of fungi of the species Rhodotorula spp., Cryptococcus spp., Exophilia spp. , Hormoconis spp. is reduced as well as the

 Enrichment of the medically relevant forms of Candida, for example C. albicans, C. boidinii, C. catenulata, C. ciferii, C. dubliniensis, C. glabrata, C. guilliermondii, C. haemulonii, C. kefyr, C. krusei, C. lipolytica, C. lusitaniae, C. norvegensis, C. parapsilosis, C. pulcherrima, C. rugosa, C. tropicalis, C. utilis, C. viswanathii. Particularly preferred are C. albicans, C. stellatoidea,

 C. tropicalis, C. glabrata and C. parapsilosis. The mycelial form of Candida is considered as a human pathogenic form of the fungus. A reduction in the

Enrichment of Candida, for example, on textiles or plastics reduces the risk of reinfection without increasing the formation of resistance.

The antimicrobial agent preparation according to the invention is suitable for reducing the accumulation of all species of the genus Aspergillus on surfaces selected from Aspergillus aculeatus, Aspergillus albus, Aspergillus alliaceus, Aspergillus asperescens, Aspergillus awamori, Aspergillus candidus, Aspergillus carbonarius, Aspergillus carneus, Aspergillus chevalieri, Aspergillus chevalieri var intermedius, Aspergillus clavatus, Aspergillus ficuum, Aspergillus flavipes,

Aspergillus flavus, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus Aspergillus parasiticus, Aspergillus parasiticus var. globosus, Aspergillus penicillioides, Aspergillus phoenicis, Aspergillus rugulosus, Aspergillus sclerotiorum, Aspergillus sojae var.

gymnosardae, Aspergillus sydowi, Aspergillus tamarii, Aspergillus terreus, Aspergillus terricola, Aspergillus toxicarius, Aspergillus unguis, Aspergillus ustus, Aspergillus versicolor, Aspergillus vitricolae and Aspergillus wentii. Particularly preferably, the accumulation of Aspergillus flavus and Aspergillus nidulans is reduced or essentially completely prevented.

The preparation of the active agent according to the invention also reduces the accumulation of human, animal and / or plant pathogenic viruses and of bacteriophages.

Other microorganisms which are particularly relevant and whose accumulation is reduced are, for example, Aeromonads, in particular Aeromonas hydrophila or Aeromonas salmonicida, Agrobacterium, in particular

Agrobacterium tumefaciens, Aquabacterium, Bradyrhizobium japonicum,

Burkholderia cepacia, Chromobacterium violaceum, Dermacoccen, in particular Dermacoccus nishinomiyaensis, Enterobacter agglomerans, Erwinia carotovora, Erwinia chrysanthemi, Escherichia coli, Nitrosomona europaea, Obesumbacterium proteus, Pantoea stewartii, Pseudomonads, in particular Pseudomonas aeruginosa, Pseudomonas aureofaciens, Pseudomonas fluorescens or Pseudomonas syringae, Ralstonia solanacearum , Rhizobium, in particular Rhizobium etli or Rhizobium leguminosarum, Rhodobacter sphaeroides, Salmonella enterica, Serratia, in particular Serratia liquefaciens, Vibrio anguillarum, Vibrio fischeri, Xanthomonas, in particular Xanthomonas campestris, Xenorhabdus nematophilus, Yersinia, in particular Yersinia enterolytica, Yersinia pestis, Yersinia pseudotuberculosis or Yersinia ruckeri.

The antimicrobial active agent preparation according to the invention acts in a detergent or cleaning agent preparation against those described Reliable microorganisms. However, according to Hohenstein, there is currently no solution to the difficult problem that dermatophyte mycelium and spores or Trichophyton mycelium and spores contribute to the washing or cleaning process

Temperatures below 60 ° C survive.

It was therefore particularly surprising that the inventive

Preparing agent enrichment of keratinophilic ascomycetes selected from the family Arthrodermataceae is reduced and that the accumulation of keratinophilic fungi selected from Trichophyton

mentagrophytes, T. rubrum, T. asteroids, T. concentrium, T. equinum, T. meginii, T. gallinae, T. tonsurans, T. schoenleinii, T. terrestre, T. verrucosum, T. violaceum, Microsporum canis, Microsporum audounii, M. gypseum, Epidermophyton flossocum, Malassezia furfur, M. sympodialis, M. globosa, and M. pachydermatis. In particular, the accumulation of

human pathogenic fungi (dermatophytes) prevented.

The active agent preparation according to the invention is preferably used in detergents and / or cleaning agents. The invention therefore also detergents and / or cleaning agents as such, which contain the antimicrobial active agent preparation according to the invention. The washing and / or cleaning agents are explained in more detail below.

As detergents and / or cleaning agents according to the application in the broadest sense surfactant-containing preparations in solid form (particles, powders, etc.), semi-solid form (pastes, etc.) and liquid form (solutions, emulsions, suspensions, gels, etc.) are considered in the With regard to a beneficial effect in the application may contain any type of surfactants and hydrogen peroxide sources, usually in addition to other components that are customary for the particular application. Examples of such surfactant-containing preparations are surfactant-containing detergent formulations, surfactant-containing

Cleaning agents for hard surfaces, or surfactant-containing

Akiviermittelzubereitungen, each of which may be solid or liquid, but also may be in a form which includes solid and liquid components or

Includes subsets of the components side by side.

The detergents and cleaning agents may additionally contain conventional ingredients, such as anionic, nonionic, cationic and amphoteric surfactants, inorganic and organic builders, special polymers

(For example, those with Cobuildereigenschaften), foam inhibitors, dyes and possibly additional perfumes (perfumes), pH adjusters, thickeners,

Polyethylene glycols, bleaching agents and hydrogen peroxide sources (such as

for example, peroxy bleach and chlorine bleach), bleach activators, bleach stabilizers, bleach catalysts, enzymes, in particular proteases, amylases or cellulases, enzyme stabilizers, dye transfer inhibitors and grayness inhibitors, without the ingredients being restricted to these substance groups. Frequently, important ingredients of these preparations are also washing aids, for example, non-limiting optical brighteners, UV-protective substances and Soil Repellents, especially polymers that counteract the re-soiling of fibers are understood. In the event that the preparations are at least partly present as shaped bodies, binding and disintegration aids may also be present.

The antimicrobial active agent preparation according to the invention is in particular in washing and / or cleaning agents in an amount of 0.5 to 30 wt .-%, preferably in an amount of 1 to 20 wt .-%, in particular in an amount of 2 to 15 wt. %, used, based in each case on the total weight of the washing and / or cleaning agent.

Detergents and / or cleaning agents according to the invention may have an acidic, neutral or basic pH. In a preferred

Embodiment have the inventive washing and / or

Detergents have a pH of 0 to 14, more preferably solid detergents have a pH above 8 and liquid detergents below 8, wherein this is due to the dilution of water or by addition of other alkaline substances during the wash cycle increased to more than 8 (see also EP 1967579).

embodiments

The bleaching systems according to the invention were each individually in a

Detergent preparation introduced and tested on various germs. The tests were analogous to the study by Betz et al. (Surfactants Surf., Det. 37 (2000), 230-234), d. H. the germ-reducing effect of the bleaching systems according to the invention in the quantitative suspension test was determined under laboratory conditions.

sample name

A 1 g IEC-A basic detergent (available from WfK GmbH);

B 1 g IEC-A plus 75 mg sodium percarbonate (Evonik);

C 1 g IEC-A plus 75 mg sodium percarbonate plus 50 mg TAED (company

 Clariant);

 D 1 g IEC-A plus 75 mg sodium percarbonate plus 50 mg DOBA (company

 Clariant);

 E 1 g IEC-A plus 75 mg sodium percarbonate plus 25 mg TAED plus 25 mg DOBA (Cogranulate, Clariant).

The samples A, B and C are each comparative examples, the samples D and E are each according to the invention preparations.

example 1

250 ml of water were heated to a temperature of 20 ° C. From the corresponding microorganism, 1 ml of the respective microbial suspension with a desired germ count of 10 ⁹ CFU / ml was added. The aspired

Germ number in the test solution is 5-10 ⁵ to 5 0 ⁶ CFU / ml (KbE: colony forming unit). The test was started by adding the sample. The test solution was heated in 1 l beakers at 20 ° C and stirred at 500 rpm (magnetic core 4 cm). Aliquots were taken at 0, 5, 10, and 15 minutes after addition of the sample and after dilution with 0.9% NaCl solution plus 1% polysorbate 80 (Tween 80, Merck) on CASO agar or Sabouraud 4% dextrose -Agar plated. In the case of the bacteria Pseudomonas aeruginosa and Staphylococcus aureus, the CASO agar was used and the agar plates were incubated at 30 ° C for a period of five days each. In the case of the yeast Candida albicans, the Sabouraud agar was used and the plates were incubated at 25 ° C for a period of seven days.

As test germs were used

Pseudomonas aeruginosa ATCC 9027, NCIMB 8626

Staphylococcus aureus ATCC 6538, NCTC 10788

Candida albicans ATCC 10231, NCPF 3179

Result

 In the following tables the decrease of the microbial load in KbE / ml test solution is dependent on the test germ and the used

Detergent preparations shown (see sample designation).

KbE / ml test solution of Pseudomonas aeruginosa depending on the detergent and the exposure time in minutes

Sample A and B reduce the germ load only very slightly. Sample C and D noticeably reduce the germ load already after five minutes and after 15 minutes all germs are eliminated up to the detection limit. Sample E reduces the microbial load after only 10 minutes to the limit of detection, indicating a synergistic effect of the two antimicrobial species

Peracetic acid and perdecanoic acid indicates. Table 2: KbE / ml test solution of Staphylococcus aureus depending on the detergent and the exposure time in minutes

Sample A and B show no germ-reducing effect on this test germ. Also, the bleach system in Sample C shows only a minor change in germ load. Surprisingly, it could be shown that samples D and E were able to reduce the complete germ load after only five minutes to the detection limit.

Table 3: Cfu / ml test solution of Candida albicans depending on the detergent and the exposure time in minutes

Sample A and B show virtually no germ-reducing effect on this test germ. Also, the bleach system in sample C shows only a small amount

Change in the germ burden. Surprisingly, it could be shown that samples D and E were able to reduce the complete microbial count to the limit of detection after just five minutes. This result is extraordinary, as Candida albicans is a problematic germ that is not easy to kill in the washing machine with conventional detergents.

Example 2

250 ml of water were heated to a temperature of 20 ° C. From the corresponding microorganism, 2.5 ml of the respective microbial suspension having a desired germ count of 10 ⁷ CFU / ml was added. The aspired Germ number in the test solution is 5 × 10 ⁴ to 5 × 10 ⁵ cfu / ml (KbE = colony-forming unit).

The test was started by adding the sample. The test solution was heated in 1 l beakers at 20 ° C and stirred at 500 rpm (magnetic core 4 cm).

Aliquots were taken at 0, 5, 10 and 15 minutes after addition of the sample and, after dilution with 0.9% NaCl solution plus 1% polysorbate 80 (Tween 80, Merck) on Sabouraud 4% dextrose agar by means of boiling 'schem Plattengussverfahren ausplatiert. The agar plates were incubated at 25 ° C for seven days each.

As a test germ was used

Trichophyton rubrum DSM 4167

Result

 The following table shows the decrease in the microbial load in CFU / ml of test solution in the detergent preparations used (see sample designation).

Table 4: KbE / ml test solution of Trichophyton rubrum depending on the detergent and the exposure time in minutes

A B C D E

0 min 2.50 · 10 ⁵ 2.70 · 10 ⁵ 2.70 · 10 ⁵ 2.20 · 10 ⁵ 2.50 · 10 ⁵

5 min 2.40 x 10 ⁵ 2.20 x 10 ⁵ 2.10 x 10 ⁵ 9.00 x 10 ⁴ 6.50 x 10 ³

10 min 2.00 × 10 ⁵ 3.10 × 10 ⁵ 2.20 × 10 ⁵ 5.00 × 10 ⁴ 5.50 × 10 ²

15 min 1.90 · 10 ⁵ 3.20 · 10 ⁵ 1.80 · 10 ⁵ 1, 80 · 10 ³ 8.00 · 10 ¹ ABCDE

0 min

5 min 4,000% 18,519% 22,222% 59,091% 97,400%

None

 10 minutes 20,000% 18,519% 77.273% 99.780%

 reduction

None

 15 min 24,000% 33,333% 99,182% 99,968%

 reduction

Sample A and B show no germ-reducing effect on this test germ. Also, the bleach system in Sample C shows only a minor change in germ load. Surprisingly, it could be shown that sample D could significantly reduce the germ load after fifteen minutes. The sample E, which contains TAED in combination with DOBA, shows a significant reduction of the germ load after only 5 minutes, after 15 minutes it is

Germ burden already reduced by three log units. Accordingly, a synergistic effect of the two antimicrobial species peracetic acid and perdecanoic acid is also shown on this germ.

Example 3

 Analogous to the previously described examples, new samples F to K were prepared as follows:

sample name

 F 1 g IEC-A basic detergent (available from WfK GmbH);

G 1 g IEC-A plus 150 mg sodium percarbonate (Evonik);

H 1 g IEC-A plus 150 mg sodium percarbonate plus 100 mg TAED

 (Clariant company);

 I 1 g IEC-A plus 150 mg sodium percarbonate plus 100 mg DOBA

(Clariant company); K 1 g IEC-A plus 150 mg sodium percarbonate plus 50 mg TAED plus 50 mg DOBA (Cogranulate, Clariant).

The samples F, G and H each represent comparative examples, the samples I and K are preparations according to the invention.

250 ml of water were heated to a temperature of 20 ° C. From the microorganism 2.5 ml of the respective microbial suspension with a target bacterial count of 10 ⁷ cfu / ml was added. The desired number of bacteria in the test solution is 5 × 10 ⁴ to 5 × 10 ⁵ cfu / ml (KbE: colony-forming unit).

The test was started by adding the sample. The test solution was heated in 1 l beakers at 20 ° C and stirred at 500 rpm (magnetic core 4 cm).

Aliquots were taken at 0, 5, 10, 15, and 20 minutes after addition of the sample, and after dilution with 0.9% NaCl solution plus 1% polysorbate 80 (Tween 80, Merck) on Subouraud 4% dextrose agar Plated by Koch'schem Plattengussverfahren. The agar plates were incubated at 25 ° C for seven days each.

As a test germ was used

Trichophyton rubrum DSM 4167 result

 The following table shows the decrease of the bacterial load in CFU / ml

Test solution in the detergent preparations used (see sample name). Table 5: Cfu / ml test solution of Trichophyton rubrum depending on the detergent and the exposure time in minutes

In this example, twice the amount of percarbonate and

Bleach activator used on the fungus Trichophyton rubrum. Samples F and G show no germ-reducing effect on this test germ. This means that even with double the amount of detergent base, here ICE-A, or

Detergent base plus percarbonate no removal of fungal mycelia and spores is expected. Microwave detergent preparations thus require a

Bleaching system, by the formation of peroxyacid an effective elimination dermatophytes guaranteed. It is important to find the right ones

Use peroxyacids.

Even in the market known system of a peroxyacetic acid, which is formed from TAED and percarbonate under alkaline conditions in the sample H, only a minor effect on the test germ. Surprisingly, it could be shown that when Peroxydecansäure is used according to sample I, the germ count is significantly reduced after 15 min and after 20 min, the germs are killed to the detection limit. The sample K, which contains TAED in combination with DOBA, shows a significant reduction of germ counts already after 10 min, after 20 min all germs are also eliminated up to the detection limit.

The results from Examples 2 and 3 impressively demonstrate that a highly effective solution has been found for the technical task of the invention.

Claims

claims: 1. Antimicrobial active ingredient preparation which is effective in particular against mycelium and spores at pH> 7, comprising: a) a hydrogen peroxide source selected from the group consisting of H ₂ O ₂ , sodium perborate and sodium percarbonate, b) an acyloxybenzoic acid (OBA) having the general chemical formula :

in the R, a linear or branched, saturated alkyl group having 6 to 22 carbon atoms, a linear or branched, mono- or polyunsaturated alkenyl group having 6 to 22 carbon atoms or an aryl group having 6 to 22 carbon atoms, and c) and optionally tetraacetylethylenediamine (TAED). 2. Antimicrobial active ingredient preparation according to claim 1, characterized in that preferred radicals R in acyloxybenzoic acid (OBA) are linear or branched saturated alkyl groups having 7 to 18 C atoms or linear or branched unsaturated alkylene groups having 7 to 18 C atoms or an aryl group having 6 to 10 C atoms. 3. Antimicrobial active ingredient preparation according to claim 1, characterized in that particularly preferred radicals R in the acyloxybenzoic acid (OBA) are linear saturated alkyl groups having 8 to 12 C atoms or linear unsaturated alkylene groups having 8 to 12 C atoms or an aryl group having 6 C atoms. 4. Antimicrobial active agent preparation according to one of claims 1 to 3, characterized in that the mixing ratio of component b), OBA, to component c), TAED, in the active agent preparation according to the invention in the range of 10: 1 to 1: 10 parts by weight, preferably in the range of 2: 1 to 1: 2. 5. antimicrobial active agent preparation according to one of claims 1 to 4, characterized in that it contains the components b) and c) in the form of a co-granule which is stabilized with a binder selected from the group consisting of waxes, silicones, fatty acids, Fatty alcohols, soaps, anionic surfactants, nonionic surfactants, cationic surfactants, anionic and cationic polymers and polyalkylene glycols, preferably C8-C31 fatty acids, which include lauric, myristic stearin fatty acid, C8-C31 fatty alcohols, Polyethylene glycols having a molecular weight of 1000 to 50 000 g / mol, Fatty alcohol polyalkoxylates having 1 to 100 moles of ethylene glycol units in the Molecule, alkanesulfonates, alkylbenzenesulfonates, alpha-olefinsulfonates, alkyl sulfates, alkyl ether sulfates with C8-C31 hydrocarbon radicals, polymers such as For example, polyvinyl alcohols, waxes such as montan waxes, paraffin waxes, ester waxes, polyolefin waxes and silicones. 6. antimicrobial active agent preparation according to one of claims 1 to 5, characterized in that the ratio of acyloxybenzoic acid (OBA) to hydrogen peroxide source in the range of 1: 1 to 1: 15, measured in molar proportions, preferably from 1: 2 to 1: 5 , more preferably 1: 3. 7. Antimicrobial active agent preparation according to one of claims 1 to 6, characterized in that the radical R in the acyloxybenzoic acid (OBA) is a linear saturated alkyl group having 10 C-atoms. 8. antimicrobial active agent preparation according to one of claims 1 to 7, characterized in that the radical R in the acyloxybenzoic acid (OBA) is a linear saturated alkyl group having 10 C-atoms and that it additionally contains component c), TAED. 9. Antimicrobial active agent preparation according to one of claims 1 to 8, characterized in that an antimicrobial effect on yeasts and fungi at a temperature in the range of 0 to 80 ° C, preferably from 10 to 60 ° C, particularly preferably from 20 to 40 ° C, occurs, in particular on keratinophilic fungi such as dermatophytes of the genus Microsporum, Trichophyton or Epidermophyton. 10. Antimicrobial active agent preparation according to claim 9, characterized in that the antimicrobial effect occurs on fungi belonging to the strain Ascomycota, the class of Eurotiomycetes, the order Onygenales and the family Arthrodermataceae, preferably of the genus Trichophyton. 11. detergents or cleaners containing an antimicrobial Active ingredient preparation according to one of claims 1 to 10, characterized in that it contains the antimicrobial active agent preparation in an amount of 0.5 to 30% by weight, preferably in an amount of 1 to 20% by weight, in particular in an amount of 2 to 15% by weight in each case on the total weight of the washing and / or cleaning agent. 12. Washing and cleaning agent according to claim 11, characterized in that it is present as a powder detergent, as a semi-liquid detergent in the form of a paste or as a liquid detergent. 13. Washing and cleaning agent according to claim 11 or 12, characterized in that at a pH of> 7 and at a temperature in the range of 10 to 60 ° C, preferably from 20 to 40 ° C, the release of peracids with at least 6 and at most 22 C atoms, preferably of at least 8 and at most 12 C atoms, is carried out, wherein the peracids block the activity of microorganisms, yeasts and fungi, including especially Dermatophytes, preferably of Trichophyton, and more preferably of Trichophyton rubrum. 14. Use of a washing and cleaning agent according to one of Claims 11 to 13 for the removal of microorganisms, in sterilization, disinfection, impregnation or preservatives, washing or Detergents, or in cooling or cooling lubricants and in the field of water purification / water treatment and the pharmaceutical, food, brewery, medical technology, paint, wood, textile, cosmetic, leather, tobacco, fur , Rope, paper, pulp, plastics, fuel, oil, rubber or machinery industries. 15. Use of a washing and cleaning agent according to one of Claims 11 to 13 for controlling the number of microorganisms in medical devices, instruments and apparatus, in particular in catheters and Endoscopes.