[go: up one dir, main page]

WO2012065075A3 - Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep) - Google Patents

Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep) Download PDF

Info

Publication number
WO2012065075A3
WO2012065075A3 PCT/US2011/060391 US2011060391W WO2012065075A3 WO 2012065075 A3 WO2012065075 A3 WO 2012065075A3 US 2011060391 W US2011060391 W US 2011060391W WO 2012065075 A3 WO2012065075 A3 WO 2012065075A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
dep
devices
dielectrophoresis
high voltage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/060391
Other languages
French (fr)
Other versions
WO2012065075A2 (en
Inventor
Michael Heller
Avery Sonnenberg
Rajaram Krishnan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2012065075A2 publication Critical patent/WO2012065075A2/en
Publication of WO2012065075A3 publication Critical patent/WO2012065075A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/024—Non-uniform field separators using high-gradient differential dielectric separation, i.e. using a dielectric matrix polarised by an external field
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Devices and techniques are disclosed that provide single and multi "pore-based" AC/DC high voltage electrokinetic devices and improved features and methods for carrying out dielectrophoresis (DEP) under high conductance conditions. The electrokinetic devices and methods allow rapid isolation, separation, detection, and analysis of cells, bacteria, virus, cell free circulating (cfc) DNA, cfc-RNA, and other cellular nanoparticulates (exosomes, etc.), and also allow drug delivery and separation of other nanoparticles to be carried out under high conductance conditions directly from blood, plasma, serum, urine, and other clinical, biological, and environmental samples in an unaltered condition.
PCT/US2011/060391 2010-11-12 2011-11-11 Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep) Ceased WO2012065075A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41330610P 2010-11-12 2010-11-12
US61/413,306 2010-11-12

Publications (2)

Publication Number Publication Date
WO2012065075A2 WO2012065075A2 (en) 2012-05-18
WO2012065075A3 true WO2012065075A3 (en) 2012-10-04

Family

ID=46051589

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/060391 Ceased WO2012065075A2 (en) 2010-11-12 2011-11-11 Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep)

Country Status (1)

Country Link
WO (1) WO2012065075A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014004563A1 (en) * 2012-06-25 2014-01-03 The Arizona Board Of Regents On Behalf Of The University Of Arizona Electrokinetic polymerase chain reaction (pcr) devices and methods
CN105241940B (en) * 2015-08-05 2018-04-27 深圳大学 A kind of Composition analyzed method and its system based on the dielectrophoresis field of force

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280590B1 (en) * 1996-09-06 2001-08-28 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US20060201811A1 (en) * 2005-03-04 2006-09-14 Hamers Robert J Apparatus for transport and analysis of particles using dielectrophoresis
WO2009146143A2 (en) * 2008-04-03 2009-12-03 The Regents Of The University Of California Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
US7658829B2 (en) * 2005-04-08 2010-02-09 Uti Limited Partnership Integrated microfluidic transport and sorting system
US7666289B2 (en) * 2005-03-11 2010-02-23 Sandia Corporation Methods and devices for high-throughput dielectrophoretic concentration

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280590B1 (en) * 1996-09-06 2001-08-28 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US20060201811A1 (en) * 2005-03-04 2006-09-14 Hamers Robert J Apparatus for transport and analysis of particles using dielectrophoresis
US7666289B2 (en) * 2005-03-11 2010-02-23 Sandia Corporation Methods and devices for high-throughput dielectrophoretic concentration
US7658829B2 (en) * 2005-04-08 2010-02-09 Uti Limited Partnership Integrated microfluidic transport and sorting system
WO2009146143A2 (en) * 2008-04-03 2009-12-03 The Regents Of The University Of California Ex-vivo multi-dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers

Also Published As

Publication number Publication date
WO2012065075A2 (en) 2012-05-18

Similar Documents

Publication Publication Date Title
Veziroglu et al. Characterizing extracellular vesicles and their diverse RNA contents
Lötvall et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles
GB2468226A (en) Ex-vivo multi dimensional system for the separation and isolation of cells, vesicles, nanoparticles and biomarkers
Lässer Identification and analysis of circulating exosomal microRNA in human body fluids
US20110124098A1 (en) Modular Microfluidic System for Biological Sample Preparation
WO2013126774A3 (en) Microfluidic devices for capture of target species
WO2010104856A3 (en) Devices and methods for contactless dielectrophoresis for cell or particle manipulation
WO2014152048A3 (en) Assemblies and methods for reducing optical crosstalk in particle processing systems
Mohd Maidin et al. Dielectrophoresis applications in biomedical field and future perspectives in biomedical technology
WO2012122564A3 (en) Diagnostic and sample preparation devices and methods
Moon et al. Dielectrophoretic separation of airborne microbes and dust particles using a microfluidic channel for real-time bioaerosol monitoring
EP2781906B1 (en) Method for identifying cells
WO2010028382A3 (en) Collecting and processing complex macromolecular mixtures
WO2007070592A3 (en) Three dimensional dielectrophorectic separator and methods of use
WO2011088014A3 (en) Systems and methods for counting cells and biomolecules
CN111778341B (en) A biomarker of active pulmonary tuberculosis and its application
MX368673B (en) Devices, systems and methods for sample preparation.
WO2011126833A3 (en) Anti-inflammatory factors
Ehrenfest et al. Selecting a relevant in vitro cell model for testing and comparing the effects of a Choukroun's platelet-rich fibrin (PRF) membrane and a platelet-rich plasma (PRP) gel: tricks and traps
WO2012166706A3 (en) Supramolecular nanofibers and hydrogels based on oligopeptides functionalized with nucleobases
del Carmen Jaramillo et al. Increasing PCR sensitivity by removal of polymerase inhibitors in environmental samples by using dielectrophoresis
WO2012065075A3 (en) Electrokinetic devices and methods for high conductance and high voltage dielectrophoresis (dep)
Xing et al. Simultaneous Isolation and Preparation of Extracellular Vesicles by Circular Multicavity Electrophoresis
WO2014184684A3 (en) Methods and biomarkers for detection of hematological cancers
WO2009034514A3 (en) Dielectrophoretic device and method for cell membrane studies

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11839306

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11839306

Country of ref document: EP

Kind code of ref document: A2