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WO2009034514A3 - Dielectrophoretic device and method for cell membrane studies - Google Patents

Dielectrophoretic device and method for cell membrane studies Download PDF

Info

Publication number
WO2009034514A3
WO2009034514A3 PCT/IB2008/053622 IB2008053622W WO2009034514A3 WO 2009034514 A3 WO2009034514 A3 WO 2009034514A3 IB 2008053622 W IB2008053622 W IB 2008053622W WO 2009034514 A3 WO2009034514 A3 WO 2009034514A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell membrane
cage
particle
particles
detection system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2008/053622
Other languages
French (fr)
Other versions
WO2009034514A2 (en
Inventor
Thomas J. De Hoog
Murray F. Gillies
Stefano Cattaneo
Dennis Merkle
Dirkjan B. Van Dam
Jérémie SIBILLE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke Philips NV
Original Assignee
Koninklijke Philips Electronics NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips Electronics NV filed Critical Koninklijke Philips Electronics NV
Publication of WO2009034514A2 publication Critical patent/WO2009034514A2/en
Publication of WO2009034514A3 publication Critical patent/WO2009034514A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A dielectrophoretic device and method is described for manipulating one or more particles, e.g. living cells. The device comprises a plurality of electrodes in the form of a DEP cage, and a particle movement detection system for detecting movement of the one or more particles within the cage. Particle movement is indicative of passing through the cross-over frequency. The particle movement detection system includes a particle presence sensor and this can be located eccentrically with respect to a trapping point of the DEP cage. The device and method may be used for classification, identification, of quantification of diseased versus healthy cells, for diagnosis, for medical research and development and in therapy. They are particularly useful for investigating living cells whose cell membrane alters in conductivity or permeability.
PCT/IB2008/053622 2007-09-10 2008-09-08 Dielectrophoretic device and method for cell membrane studies Ceased WO2009034514A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07116010.5 2007-09-10
EP07116010A EP2042239A1 (en) 2007-09-10 2007-09-10 Dielectrophoretic device and method for cell membrane studies

Publications (2)

Publication Number Publication Date
WO2009034514A2 WO2009034514A2 (en) 2009-03-19
WO2009034514A3 true WO2009034514A3 (en) 2009-10-22

Family

ID=39107761

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/053622 Ceased WO2009034514A2 (en) 2007-09-10 2008-09-08 Dielectrophoretic device and method for cell membrane studies

Country Status (2)

Country Link
EP (1) EP2042239A1 (en)
WO (1) WO2009034514A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102166506A (en) * 2011-03-02 2011-08-31 厦门大学 Method for confining alternating current electric field of charged particles in solution
EP2781906B1 (en) 2013-03-19 2021-08-18 IMEC vzw Method for identifying cells
WO2015157072A1 (en) * 2014-04-09 2015-10-15 Apocell, Inc. System and method for determining dielectrophoresis crossover frequencies
CN103985428B (en) * 2014-05-15 2017-02-15 清华大学 Molecule three-dimensional rotation operation device based on dielectrophoresis and control method of molecule three-dimensional rotation operation device based on dielectrophoresis
JP6441838B2 (en) * 2016-01-29 2018-12-19 株式会社Ａｆｉテクノロジー Analysis device and separation device
US11686704B2 (en) * 2021-02-10 2023-06-27 Taiwan Semiconductor Manufacturing Company, Ltd. Biosensor

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998004355A1 (en) * 1996-07-26 1998-02-05 Btg International Limited Apparatus and method for testing particles using dielectrophoresis
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1145766A2 (en) * 2000-04-13 2001-10-17 Wako Pure Chemical Industries Ltd Electrode construction for dielectrophoretic apparatus and separation by dielectrophoresis
US20020036142A1 (en) * 2000-06-14 2002-03-28 Peter Gascoyne Systems and methods for cell subpopulation analysis
US20040178068A1 (en) * 2002-12-20 2004-09-16 The University Of Texas System Methods and apparatus for electrosmear analysis
US6801311B1 (en) * 1999-01-26 2004-10-05 Evotec Technologies Gmbh Process and device for the detection of microscopically small objects
WO2005031300A2 (en) * 2003-06-27 2005-04-07 Purdue Research Foundation Device for detecting biological and chemical particles
US20060219939A1 (en) * 2004-12-03 2006-10-05 Nano Science Diagnostic, Inc. Method and apparatus for low quantity detection of bioparticles in small sample volumes
WO2007010367A2 (en) * 2005-07-19 2007-01-25 Silicon Biosystems S.P.A. Method and apparatus for the manipulation and/or the detection of particles

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998004355A1 (en) * 1996-07-26 1998-02-05 Btg International Limited Apparatus and method for testing particles using dielectrophoresis
US6801311B1 (en) * 1999-01-26 2004-10-05 Evotec Technologies Gmbh Process and device for the detection of microscopically small objects
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
EP1145766A2 (en) * 2000-04-13 2001-10-17 Wako Pure Chemical Industries Ltd Electrode construction for dielectrophoretic apparatus and separation by dielectrophoresis
US20020036142A1 (en) * 2000-06-14 2002-03-28 Peter Gascoyne Systems and methods for cell subpopulation analysis
US20040178068A1 (en) * 2002-12-20 2004-09-16 The University Of Texas System Methods and apparatus for electrosmear analysis
WO2005031300A2 (en) * 2003-06-27 2005-04-07 Purdue Research Foundation Device for detecting biological and chemical particles
US20060219939A1 (en) * 2004-12-03 2006-10-05 Nano Science Diagnostic, Inc. Method and apparatus for low quantity detection of bioparticles in small sample volumes
WO2007010367A2 (en) * 2005-07-19 2007-01-25 Silicon Biosystems S.P.A. Method and apparatus for the manipulation and/or the detection of particles

Also Published As

Publication number Publication date
WO2009034514A2 (en) 2009-03-19
EP2042239A1 (en) 2009-04-01

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