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WO2011086189A3 - Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries - Google Patents

Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries Download PDF

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Publication number
WO2011086189A3
WO2011086189A3 PCT/EP2011/050555 EP2011050555W WO2011086189A3 WO 2011086189 A3 WO2011086189 A3 WO 2011086189A3 EP 2011050555 W EP2011050555 W EP 2011050555W WO 2011086189 A3 WO2011086189 A3 WO 2011086189A3
Authority
WO
WIPO (PCT)
Prior art keywords
cyanobacteria
fatty alcohols
synthesis
constructs
vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2011/050555
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English (en)
Other versions
WO2011086189A2 (fr
Inventor
Xuefeng Lu
Xiaoming Tan
Fengxia Qi
Quan LUO
Lun Yao
Qianqian Gao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shell Internationale Research Maatschappij BV
Original Assignee
Shell Internationale Research Maatschappij BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201010034404.4A external-priority patent/CN102127563B/zh
Priority claimed from CN201010213758.5A external-priority patent/CN102311966B/zh
Application filed by Shell Internationale Research Maatschappij BV filed Critical Shell Internationale Research Maatschappij BV
Priority to EP11700428A priority Critical patent/EP2524035A2/fr
Priority to BR112012017456A priority patent/BR112012017456A2/pt
Priority to CA2786244A priority patent/CA2786244A1/fr
Publication of WO2011086189A2 publication Critical patent/WO2011086189A2/fr
Publication of WO2011086189A3 publication Critical patent/WO2011086189A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Liquid Carbonaceous Fuels (AREA)

Abstract

La présente invention concerne des produits de recombinaison, des vecteurs et des cyanobactéries pour la synthèse d'alcools gras, et des procédés de production d'alcools gras dans des cyanobactéries. Spécifiquement, la présente invention concerne un produit de recombinaison pour la synthèse d'alcools gras dans des cyanobactéries, un vecteur comprenant le produit de recombinaison, une cyanobactérie comprenant le produit de recombinaison ou transformée par le vecteur, et un procédé de production d'alcools gras dans des cyanobactéries.
PCT/EP2011/050555 2010-01-15 2011-01-17 Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries Ceased WO2011086189A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP11700428A EP2524035A2 (fr) 2010-01-15 2011-01-17 Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries
BR112012017456A BR112012017456A2 (pt) 2010-01-15 2011-01-17 "construto, vetor, cianobact´peria, método para produzir alcoóis graxos em uma cianobactéria, biocombustível, e, método de expressão de genes exógenos"
CA2786244A CA2786244A1 (fr) 2010-01-15 2011-01-17 Produits de recombinaison, vecteurs et cyanobacteries pour la synthese d'alcools gras, et procedes de production d'alcools gras dans des cyanobacteries

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201010034404.4 2010-01-15
CN201010034404.4A CN102127563B (zh) 2010-01-15 2010-01-15 一种集胞藻pcc6803表达外源基因的方法
CN201010213758.5A CN102311966B (zh) 2010-06-30 2010-06-30 用于合成脂肪醇的构建体，载体，蓝细菌，以及在蓝细菌中生产脂肪醇的方法
CN201010213758.5 2010-06-30

Publications (2)

Publication Number Publication Date
WO2011086189A2 WO2011086189A2 (fr) 2011-07-21
WO2011086189A3 true WO2011086189A3 (fr) 2011-10-13

Family

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Family Applications (1)

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PCT/EP2011/050555 Ceased WO2011086189A2 (fr) 2010-01-15 2011-01-17 Produits de recombinaison, vecteurs et cyanobactéries pour la synthèse d'alcools gras, et procédés de production d'alcools gras dans des cyanobactéries

Country Status (4)

Country Link
EP (1) EP2524035A2 (fr)
BR (1) BR112012017456A2 (fr)
CA (1) CA2786244A1 (fr)
WO (1) WO2011086189A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235172B (zh) * 2020-02-24 2021-10-19 扬州大学 基于lacZ基因和pUC复制子的原核启动子报告系统及其构建方法和应用

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013029027A1 (fr) * 2011-08-24 2013-02-28 Algenol Biofuels Inc. Séparation de la biomasse productive de la biomasse non productive dans des bioréacteurs et procédés associés
CN102952818B (zh) 2011-08-26 2016-03-02 中国科学院青岛生物能源与过程研究所 用于在蓝细菌中提高脂肪醇产量的构建体和方法
CN109097378B (zh) * 2018-08-13 2021-08-03 中国科学院青岛生物能源与过程研究所 一种异戊二烯合酶和其编码基因、表达载体、工程菌以及生产异戊二烯的方法及应用
CN111041038B (zh) * 2019-12-02 2022-11-08 天津大学 高效生物合成虾青素的集胞藻6803基因工程菌及构建方法及应用
CN111549047A (zh) * 2020-04-20 2020-08-18 山东大学 驱动外源基因在蓝藻中高效表达的启动子的筛选方法
CN111560340B (zh) * 2020-04-21 2022-08-23 天津大学 一种生物合成肌醇的集胞藻基因工程菌及构建方法及应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143538A (en) * 1997-02-20 2000-11-07 The United States Of America As Represented By The United States Department Of Energy Fatty acyl-CoA reductase
WO2007136762A2 (fr) * 2006-05-19 2007-11-29 Ls9, Inc. Production d'acides gras et de leurs dérivés
WO2008119082A2 (fr) * 2007-03-28 2008-10-02 Ls9, Inc. Production améliorée de dérivés d'acides gras
WO2011019858A1 (fr) * 2009-08-11 2011-02-17 Synthetic Genomics, Inc. Production microbienne d'alcools gras

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143538A (en) * 1997-02-20 2000-11-07 The United States Of America As Represented By The United States Department Of Energy Fatty acyl-CoA reductase
WO2007136762A2 (fr) * 2006-05-19 2007-11-29 Ls9, Inc. Production d'acides gras et de leurs dérivés
WO2008119082A2 (fr) * 2007-03-28 2008-10-02 Ls9, Inc. Production améliorée de dérivés d'acides gras
WO2011019858A1 (fr) * 2009-08-11 2011-02-17 Synthetic Genomics, Inc. Production microbienne d'alcools gras

Non-Patent Citations (11)

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Title
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CHEN Z ET AL: "Expression of mammalian metallothionein-I gene in cyanobacteria to enhance heavy metal resistance", MARINE POLLUTION BULLETIN, vol. 39, no. 1-12, January 1999 (1999-01-01), pages 155 - 158, XP002634176, ISSN: 0025-326X *
DOAN T T P ET AL: "Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli", JOURNAL OF PLANT PHYSIOLOGY, vol. 166, no. 8, 15 May 2009 (2009-05-15), pages 787 - 796, XP026056154, ISSN: 0176-1617, [retrieved on 20081204], DOI: DOI:10.1016/J.JPLPH.2008.10.003 *
KOKSHAROVA ET AL: "Genetic tools for cyanobacteria.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 58, no. 2, 1 February 2002 (2002-02-01), pages 123 - 137, XP055004743, ISSN: 0175-7598 *
LEE DAE-WEON: "Mosquito larvicidal activity of Synechocystis PCC6803 transformed with the cry11Aa gene to Culex tritaeniorhynchus and Anopheles sinensis", KOREAN JOURNAL OF APPLIED ENTOMOLOGY, vol. 43, no. 1, 1 March 2004 (2004-03-01), pages 35 - 41, XP008135831, ISSN: 1225-0171 *
LU ET AL: "A perspective: Photosynthetic production of fatty acid-based biofuels in genetically engineered cyanobacteria", BIOTECHNOLOGY ADVANCES, ELSEVIER PUBLISHING, BARKING, GB, vol. 28, no. 6, 1 November 2010 (2010-11-01), pages 742 - 746, XP027331813, ISSN: 0734-9750, [retrieved on 20100601] *
MERMET-BOUVIER P ET AL: "A CONDITIONAL EXPRESSION VECTOR FOR THE CYANOBACTERIA SYNECHOCYSTISSP. STRAINS PCC6803 AND PCC6714 OR SYNECHOCOCCUS SP. STRAINS PCC7942 AND PCC6301", CURRENT MICROBIOLOGY, vol. 28, no. 3, 1 January 1994 (1994-01-01), pages 145 - 148, XP002067945, ISSN: 0343-8651, DOI: DOI:10.1007/BF01571055 *
PENGCHENG FU: "Genome-scale modeling of Synechocystis sp. PCC 6803 and prediction of pathway insertion", JOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY,, vol. 84, 1 January 2008 (2008-01-01), pages 473 - 483, XP002579652, DOI: DOI:10.1002/JCTB.2065 *
SERGEYENKO TATIANA V ET AL: "Identification of secreted proteins of the cyanobacterium Synechocystis sp. strain PCC 6803", FEMS MICROBIOLOGY LETTERS, vol. 193, no. 2, 15 December 2000 (2000-12-15), pages 213 - 216, XP002634175, ISSN: 0378-1097 *
TAKESHIMA Y ET AL: "A NOVEL EXPRESSION VECTOR FOR THE CYANOBACTERIUM, SYNECHOCOCCUS PCC 6301", DNA RESEARCH, vol. 1, no. 4, 1 January 1994 (1994-01-01), pages 181 - 189, XP008039793, ISSN: 1340-2838, DOI: DOI:10.1093/DNARES/1.4.181 *
TAN XIAOMING ET AL: "Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria.", METABOLIC ENGINEERING, vol. 13, no. 2, March 2011 (2011-03-01), pages 169 - 176, XP002634177, ISSN: 1096-7184 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235172B (zh) * 2020-02-24 2021-10-19 扬州大学 基于lacZ基因和pUC复制子的原核启动子报告系统及其构建方法和应用

Also Published As

Publication number Publication date
BR112012017456A2 (pt) 2015-09-15
CA2786244A1 (fr) 2011-07-21
EP2524035A2 (fr) 2012-11-21
WO2011086189A2 (fr) 2011-07-21

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