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WO2011086189A3 - Constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria - Google Patents

Constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria Download PDF

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Publication number
WO2011086189A3
WO2011086189A3 PCT/EP2011/050555 EP2011050555W WO2011086189A3 WO 2011086189 A3 WO2011086189 A3 WO 2011086189A3 EP 2011050555 W EP2011050555 W EP 2011050555W WO 2011086189 A3 WO2011086189 A3 WO 2011086189A3
Authority
WO
WIPO (PCT)
Prior art keywords
cyanobacteria
fatty alcohols
synthesis
constructs
vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2011/050555
Other languages
French (fr)
Other versions
WO2011086189A2 (en
Inventor
Xuefeng Lu
Xiaoming Tan
Fengxia Qi
Quan LUO
Lun Yao
Qianqian Gao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shell Internationale Research Maatschappij BV
Original Assignee
Shell Internationale Research Maatschappij BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201010034404.4A external-priority patent/CN102127563B/en
Priority claimed from CN201010213758.5A external-priority patent/CN102311966B/en
Application filed by Shell Internationale Research Maatschappij BV filed Critical Shell Internationale Research Maatschappij BV
Priority to EP11700428A priority Critical patent/EP2524035A2/en
Priority to BR112012017456A priority patent/BR112012017456A2/en
Priority to CA2786244A priority patent/CA2786244A1/en
Publication of WO2011086189A2 publication Critical patent/WO2011086189A2/en
Publication of WO2011086189A3 publication Critical patent/WO2011086189A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Liquid Carbonaceous Fuels (AREA)

Abstract

The present invention relates to constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria. Specifically, the present disclosure relates to a construct for the synthesis of fatty alcohols in cyanobacteria, a vector comprising the construct, a cyanobacterium comprising the construct or transformed by the vector, and a method for producing fatty alcohols in cyanobacteria.
PCT/EP2011/050555 2010-01-15 2011-01-17 Constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria Ceased WO2011086189A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP11700428A EP2524035A2 (en) 2010-01-15 2011-01-17 Constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria
BR112012017456A BR112012017456A2 (en) 2010-01-15 2011-01-17 "construct, vector, cyanobacteria, method for producing fatty alcohols in a cyanobacterium, biofuel, and exogenous gene expression method"
CA2786244A CA2786244A1 (en) 2010-01-15 2011-01-17 Constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201010034404.4 2010-01-15
CN201010034404.4A CN102127563B (en) 2010-01-15 2010-01-15 A kind of method of expressing exogenous gene by using synechocystis pevalekii PCC 6803
CN201010213758.5A CN102311966B (en) 2010-06-30 2010-06-30 For the synthesis of the construct of fatty alcohol, carrier, cyanobacteria, and the method for producing fatty alcohol in cyanobacteria
CN201010213758.5 2010-06-30

Publications (2)

Publication Number Publication Date
WO2011086189A2 WO2011086189A2 (en) 2011-07-21
WO2011086189A3 true WO2011086189A3 (en) 2011-10-13

Family

ID=43859690

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/050555 Ceased WO2011086189A2 (en) 2010-01-15 2011-01-17 Constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria

Country Status (4)

Country Link
EP (1) EP2524035A2 (en)
BR (1) BR112012017456A2 (en)
CA (1) CA2786244A1 (en)
WO (1) WO2011086189A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235172B (en) * 2020-02-24 2021-10-19 扬州大学 Prokaryotic promoter reporter system based on lacZ gene and pUC replicon and its construction method and application

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013029027A1 (en) * 2011-08-24 2013-02-28 Algenol Biofuels Inc. Separation of productive biomass from non-productive biomass in bioreactors and methods thereof
CN102952818B (en) 2011-08-26 2016-03-02 中国科学院青岛生物能源与过程研究所 For improving construct and the method for fatty alcohol yield in cyanobacteria
CN109097378B (en) * 2018-08-13 2021-08-03 中国科学院青岛生物能源与过程研究所 A kind of isoprene synthase and its encoding gene, expression vector, engineering bacteria and method and application of producing isoprene
CN111041038B (en) * 2019-12-02 2022-11-08 天津大学 Synechocystis 6803 genetically engineered bacteria for efficient biosynthesis of astaxanthin and its construction method and application
CN111549047A (en) * 2020-04-20 2020-08-18 山东大学 Screening method of promoter for driving efficient expression of exogenous gene in blue algae
CN111560340B (en) * 2020-04-21 2022-08-23 天津大学 Synechocystis genetically engineered bacterium for biosynthesis of inositol, and construction method and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143538A (en) * 1997-02-20 2000-11-07 The United States Of America As Represented By The United States Department Of Energy Fatty acyl-CoA reductase
WO2007136762A2 (en) * 2006-05-19 2007-11-29 Ls9, Inc. Production of fatty acids and derivatives thereof
WO2008119082A2 (en) * 2007-03-28 2008-10-02 Ls9, Inc. Enhanced production of fatty acid derivatives
WO2011019858A1 (en) * 2009-08-11 2011-02-17 Synthetic Genomics, Inc. Microbial production of fatty alcohols

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143538A (en) * 1997-02-20 2000-11-07 The United States Of America As Represented By The United States Department Of Energy Fatty acyl-CoA reductase
WO2007136762A2 (en) * 2006-05-19 2007-11-29 Ls9, Inc. Production of fatty acids and derivatives thereof
WO2008119082A2 (en) * 2007-03-28 2008-10-02 Ls9, Inc. Enhanced production of fatty acid derivatives
WO2011019858A1 (en) * 2009-08-11 2011-02-17 Synthetic Genomics, Inc. Microbial production of fatty alcohols

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ANGERMAYR S A ET AL: "Energy biotechnology with cyanobacteria", CURRENT OPINION IN BIOTECHNOLOGY, vol. 20, no. 3, 1 June 2009 (2009-06-01), pages 257 - 263, XP026283526, ISSN: 0958-1669, [retrieved on 20090617], DOI: DOI:10.1016/J.COPBIO.2009.05.011 *
CHEN Z ET AL: "Expression of mammalian metallothionein-I gene in cyanobacteria to enhance heavy metal resistance", MARINE POLLUTION BULLETIN, vol. 39, no. 1-12, January 1999 (1999-01-01), pages 155 - 158, XP002634176, ISSN: 0025-326X *
DOAN T T P ET AL: "Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli", JOURNAL OF PLANT PHYSIOLOGY, vol. 166, no. 8, 15 May 2009 (2009-05-15), pages 787 - 796, XP026056154, ISSN: 0176-1617, [retrieved on 20081204], DOI: DOI:10.1016/J.JPLPH.2008.10.003 *
KOKSHAROVA ET AL: "Genetic tools for cyanobacteria.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 58, no. 2, 1 February 2002 (2002-02-01), pages 123 - 137, XP055004743, ISSN: 0175-7598 *
LEE DAE-WEON: "Mosquito larvicidal activity of Synechocystis PCC6803 transformed with the cry11Aa gene to Culex tritaeniorhynchus and Anopheles sinensis", KOREAN JOURNAL OF APPLIED ENTOMOLOGY, vol. 43, no. 1, 1 March 2004 (2004-03-01), pages 35 - 41, XP008135831, ISSN: 1225-0171 *
LU ET AL: "A perspective: Photosynthetic production of fatty acid-based biofuels in genetically engineered cyanobacteria", BIOTECHNOLOGY ADVANCES, ELSEVIER PUBLISHING, BARKING, GB, vol. 28, no. 6, 1 November 2010 (2010-11-01), pages 742 - 746, XP027331813, ISSN: 0734-9750, [retrieved on 20100601] *
MERMET-BOUVIER P ET AL: "A CONDITIONAL EXPRESSION VECTOR FOR THE CYANOBACTERIA SYNECHOCYSTISSP. STRAINS PCC6803 AND PCC6714 OR SYNECHOCOCCUS SP. STRAINS PCC7942 AND PCC6301", CURRENT MICROBIOLOGY, vol. 28, no. 3, 1 January 1994 (1994-01-01), pages 145 - 148, XP002067945, ISSN: 0343-8651, DOI: DOI:10.1007/BF01571055 *
PENGCHENG FU: "Genome-scale modeling of Synechocystis sp. PCC 6803 and prediction of pathway insertion", JOURNAL OF CHEMICAL TECHNOLOGY & BIOTECHNOLOGY,, vol. 84, 1 January 2008 (2008-01-01), pages 473 - 483, XP002579652, DOI: DOI:10.1002/JCTB.2065 *
SERGEYENKO TATIANA V ET AL: "Identification of secreted proteins of the cyanobacterium Synechocystis sp. strain PCC 6803", FEMS MICROBIOLOGY LETTERS, vol. 193, no. 2, 15 December 2000 (2000-12-15), pages 213 - 216, XP002634175, ISSN: 0378-1097 *
TAKESHIMA Y ET AL: "A NOVEL EXPRESSION VECTOR FOR THE CYANOBACTERIUM, SYNECHOCOCCUS PCC 6301", DNA RESEARCH, vol. 1, no. 4, 1 January 1994 (1994-01-01), pages 181 - 189, XP008039793, ISSN: 1340-2838, DOI: DOI:10.1093/DNARES/1.4.181 *
TAN XIAOMING ET AL: "Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria.", METABOLIC ENGINEERING, vol. 13, no. 2, March 2011 (2011-03-01), pages 169 - 176, XP002634177, ISSN: 1096-7184 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111235172B (en) * 2020-02-24 2021-10-19 扬州大学 Prokaryotic promoter reporter system based on lacZ gene and pUC replicon and its construction method and application

Also Published As

Publication number Publication date
BR112012017456A2 (en) 2015-09-15
CA2786244A1 (en) 2011-07-21
EP2524035A2 (en) 2012-11-21
WO2011086189A2 (en) 2011-07-21

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