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WO2009115660A3 - En masse selection of cells expressing extracellular enzymes in a compartmented selective environment - Google Patents

En masse selection of cells expressing extracellular enzymes in a compartmented selective environment Download PDF

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Publication number
WO2009115660A3
WO2009115660A3 PCT/FR2009/000112 FR2009000112W WO2009115660A3 WO 2009115660 A3 WO2009115660 A3 WO 2009115660A3 FR 2009000112 W FR2009000112 W FR 2009000112W WO 2009115660 A3 WO2009115660 A3 WO 2009115660A3
Authority
WO
WIPO (PCT)
Prior art keywords
selection
compartmented
masse
cells expressing
extracellular enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2009/000112
Other languages
French (fr)
Other versions
WO2009115660A2 (en
Inventor
Julien Sylvestre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2009115660A2 publication Critical patent/WO2009115660A2/en
Publication of WO2009115660A3 publication Critical patent/WO2009115660A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Pathology (AREA)
  • Botany (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method for the in vivo selection of live cells of microorganisms on the basis of the enzyme activity expressed by said cells outside the cytoplasm of their cytoplasm. The invention uses a partition system for the selection, and can particularly be used for cellulases.
PCT/FR2009/000112 2008-01-31 2009-02-02 In vivo selection Ceased WO2009115660A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0800516 2008-01-31
FR0800516A FR2927090A1 (en) 2008-01-31 2008-01-31 MASS SELECTION OF CELLS EXTRATING EXTRACELLULAR ENZYMES IN A COMPARTMENTALLY SELECTIVE MEDIUM

Publications (2)

Publication Number Publication Date
WO2009115660A2 WO2009115660A2 (en) 2009-09-24
WO2009115660A3 true WO2009115660A3 (en) 2009-12-17

Family

ID=40352020

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2009/000112 Ceased WO2009115660A2 (en) 2008-01-31 2009-02-02 In vivo selection

Country Status (2)

Country Link
FR (1) FR2927090A1 (en)
WO (1) WO2009115660A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2474609A1 (en) 2011-01-05 2012-07-11 Stichting voor de Technische Wetenschappen A method for the adaptive evolution of a cell and selection for a desired phenotype by serial propagation in an emulsion based system and uses thereof
FR3003269A1 (en) * 2013-03-12 2014-09-19 Julien Sylvestre METHOD OF CULTURE AND SELECTION OF MICROORGANISMS AND IMPLEMENTATION PLANT
WO2018037049A1 (en) 2016-08-26 2018-03-01 Nip B.V. A method of producing mixed microbial cultures

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997037036A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Compartmentalization method for screening microorganisms

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997037036A1 (en) * 1996-03-29 1997-10-09 Genencor International, Inc. Compartmentalization method for screening microorganisms

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BERNATH K; HAI M; MASTROBATTISTA E; GRIFFITHS A D; MAGDASSI S; TAWFIK D S: "In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC, NEW YORK, vol. 325, no. 1, 1 February 2004 (2004-02-01), pages 151 - 157, XP004483545, ISSN: 0003-2697 *
FAN Z ET AL: "Theoretical analysis of selection-based strain improvement for microorganisms with growth dependent upon extracytoplasmic enzymes", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 92, no. 1, 5 October 2005 (2005-10-05), pages 35 - 44, XP002353624, ISSN: 0006-3592 *
LELIEVELD H L M: "The use of continuous cultures for selection and isolation of microorganisms producing extracellular enzymes adapted to extreme environments", BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 24, no. 6, 1 January 1982 (1982-01-01), pages 1419 - 1426, XP002353623, ISSN: 0006-3592 *
NAKI D ET AL: "SELECTION OF A SUBTILISIN-HYPERPRODUCING BACILLUS IN A HIGHLY STRUCTURED ENVIRONMENT", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 49, no. 3, 1 March 1998 (1998-03-01), pages 290 - 294, XP000972404, ISSN: 0175-7598 *
SCHALLMEY MARCUS ET AL: "Developments in the use of Bacillus species for industrial production", CANADIAN JOURNAL OF MICROBIOLOGY, OTTAWA, vol. 50, no. 1, 1 January 2004 (2004-01-01), pages 1 - 17, XP009075662, ISSN: 0008-4166 *

Also Published As

Publication number Publication date
WO2009115660A2 (en) 2009-09-24
FR2927090A1 (en) 2009-08-07

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