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WO2008003053A3 - Procédés et systèmes de minimisation d'effets de diaphonie de pigments - Google Patents

Procédés et systèmes de minimisation d'effets de diaphonie de pigments Download PDF

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Publication number
WO2008003053A3
WO2008003053A3 PCT/US2007/072383 US2007072383W WO2008003053A3 WO 2008003053 A3 WO2008003053 A3 WO 2008003053A3 US 2007072383 W US2007072383 W US 2007072383W WO 2008003053 A3 WO2008003053 A3 WO 2008003053A3
Authority
WO
WIPO (PCT)
Prior art keywords
dye
signals
residual error
crosstalk
minimize
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/072383
Other languages
English (en)
Other versions
WO2008003053A2 (fr
Inventor
Stephen J Gunstream
David C Woo
John P Bodeau
Coy Mark A Mc
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Priority to EP07812433.6A priority Critical patent/EP2044432B1/fr
Publication of WO2008003053A2 publication Critical patent/WO2008003053A2/fr
Publication of WO2008003053A3 publication Critical patent/WO2008003053A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La présente invention concerne des procédés et des systèmes destinés à traiter des signaux pour minimiser les effets d'une diaphonie de pigments. Une déconvolution de signaux de pigment multiplexés comprend des corrections d'erreur résiduelle déterminées à partir de mesures expérimentales. Les corrections d'erreur résiduelle prennent en compte des facteurs propres à une réaction ou un essai et modifient le filtrage ultérieur de signaux. Des valeurs de correction sont déterminées pour des conjugués de pigment-sonde spécifiques afin de minimiser la diaphonie des pigments et combinées à une correction d'erreur résiduelle pour minimiser davantage des erreurs de déconvolution spectrale. Un appareil, des systèmes et des supports informatiques permettent de traiter des signaux et de modifier des filtres selon des valeurs obtenues au moyen des procédés.
PCT/US2007/072383 2006-06-28 2007-06-28 Procédés et systèmes de minimisation d'effets de diaphonie de pigments Ceased WO2008003053A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07812433.6A EP2044432B1 (fr) 2006-06-28 2007-06-28 Procedes et systemes de minimisation d'effets de diaphonie de pigments

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80606106P 2006-06-28 2006-06-28
US60/806,061 2006-06-28

Publications (2)

Publication Number Publication Date
WO2008003053A2 WO2008003053A2 (fr) 2008-01-03
WO2008003053A3 true WO2008003053A3 (fr) 2008-10-09

Family

ID=38846555

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/072383 Ceased WO2008003053A2 (fr) 2006-06-28 2007-06-28 Procédés et systèmes de minimisation d'effets de diaphonie de pigments

Country Status (3)

Country Link
US (1) US7839507B2 (fr)
EP (1) EP2044432B1 (fr)
WO (1) WO2008003053A2 (fr)

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US8195328B2 (en) * 2003-09-19 2012-06-05 Vesta Medical, Llc Combination disposal and dispensing apparatus and method
CN101688841B (zh) * 2007-06-29 2011-12-07 霍夫曼-拉罗奇有限公司 用于确定pcr和其他数据集中的串扰系数的系统和方法
US9121055B2 (en) * 2008-04-24 2015-09-01 3M Innovative Properties Company Analysis of nucleic acid amplification curves using wavelet transformation
US9938569B2 (en) 2009-09-10 2018-04-10 Diasorin S.P.A. Compensation for spectral crosstalk in multiplex nucleic acid amplification
US9506112B2 (en) 2010-02-05 2016-11-29 Siemens Healthcare Diagnostics Inc. Increasing multiplex level by externalization of passive reference in polymerase chain reactions
JP5985140B2 (ja) 2010-04-28 2016-09-06 ソニー株式会社 蛍光強度補正方法、蛍光強度算出方法及び蛍光強度算出装置
CN103403533B (zh) 2011-02-24 2017-02-15 简.探针公司 用于分辨光信号检测器中不同调制频率的光信号的系统和方法
KR101969846B1 (ko) 2012-06-28 2019-04-17 삼성전자주식회사 Ｐｃｒ 데이터 및 다른 데이터에서 크로스토크의 영향을 보상하여 핵산을 분석하는 방법 및 장치
HUE058723T2 (hu) 2012-11-19 2022-09-28 Apton Biosystems Inc Molekuláris analitok digitális elemzése egyetlen molekula kimutatásával
US10829816B2 (en) 2012-11-19 2020-11-10 Apton Biosystems, Inc. Methods of analyte detection
EP4306650A3 (fr) 2013-08-22 2024-03-27 Pacific Biosciences of California, Inc. Analyse numérique d'analytes moléculaires à l'aide de procédés électriques
US9170198B2 (en) * 2013-12-19 2015-10-27 Luminex Corporation Crosstalk reduction
BE1022048B1 (nl) 2014-04-11 2016-02-10 Ugentec Bvba Methoden voor fluorescentie data correctie
JP6496416B2 (ja) 2015-02-06 2019-04-03 ライフ テクノロジーズ コーポレーション 純粋な染料計器を正常化する方法およびシステム
RU2017131051A (ru) * 2015-02-06 2019-03-06 Лайф Текнолоджиз Корпорейшн Система и способы для калибровки связывающих красителей
CA3056765C (fr) 2017-03-17 2024-04-02 Apton Biosystems, Inc. Sequencage et imagerie haute resolution
EP3803339B1 (fr) * 2018-06-08 2025-02-19 Revvity Health Sciences, Inc. Étalonnage de systèmes d'analyse multispectrale
JP7022670B2 (ja) * 2018-09-10 2022-02-18 株式会社日立ハイテク スペクトル校正装置及びスペクトル校正方法
JP2022501026A (ja) 2018-09-19 2022-01-06 アプトン バイオシステムズ インコーポレイテッド 高密度に詰め込まれた分析物の層および検出方法
GB2619179B (en) * 2019-05-22 2024-03-27 Hitachi High Tech Corp Analysis device and analysis method
DE102020207587A1 (de) * 2020-06-18 2021-12-23 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Steuergerät zum Auswerten eines Lumineszenzsignals in einem Analysegerät zum Analysieren einer Probe biologischen Materials und Analysegerät zum Analysieren einer Probe biologischen Materials
EP4466529A1 (fr) * 2022-01-21 2024-11-27 IntegenX Inc. Système d'étalonnage spectral adaptatif
WO2025006242A1 (fr) * 2023-06-30 2025-01-02 Life Technologies Corporation Ajustement automatique de matrice de colorant spectral pour réduire la diaphonie spectrale dans des dosages qpcr multiplexés
CN117434044B (zh) * 2023-12-19 2024-03-08 鲲鹏基因（北京）科技有限责任公司 一种pcr仪荧光串扰系数标定方法及装置与应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6333501B1 (en) * 2000-01-27 2001-12-25 Perkin-Elmer Corporation Methods, apparatus, and articles of manufacture for performing spectral calibration
US6863791B1 (en) * 2000-09-11 2005-03-08 Spectrumedix Llc Method for in-situ calibration of electrophoretic analysis systems
US7035739B2 (en) * 2002-02-01 2006-04-25 Rosetta Inpharmatics Llc Computer systems and methods for identifying genes and determining pathways associated with traits

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US7209836B1 (en) * 1999-07-16 2007-04-24 Perkinelmer Las, Inc. Method and system for automatically creating crosstalk-corrected data of a microarray
US6418383B1 (en) * 2000-02-11 2002-07-09 Perkinelmer Instruments Llc Method and apparatus for iterative spectral compensation
US6598013B1 (en) * 2001-07-31 2003-07-22 University Of Maine Method for reducing cross-talk within DNA data
US7435602B2 (en) * 2002-12-20 2008-10-14 Applied Biosystems Inc. Reducing effects of spectral nonuniformity
EP1636730A2 (fr) * 2003-06-18 2006-03-22 Applera Corporation Procedes et systemes d'analyse de donnees de sequences biologiques
US7630849B2 (en) * 2005-09-01 2009-12-08 Applied Biosystems, Llc Method of automated calibration and diagnosis of laboratory instruments

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6333501B1 (en) * 2000-01-27 2001-12-25 Perkin-Elmer Corporation Methods, apparatus, and articles of manufacture for performing spectral calibration
US6863791B1 (en) * 2000-09-11 2005-03-08 Spectrumedix Llc Method for in-situ calibration of electrophoretic analysis systems
US7035739B2 (en) * 2002-02-01 2006-04-25 Rosetta Inpharmatics Llc Computer systems and methods for identifying genes and determining pathways associated with traits

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEWIN ET AL.: "Quantitative DNA methylation analysis based on four-dye trace data from direct sequencing of PCR amplificates", BIOINFORMATICS, vol. 20, 2004, pages 3005 - 3012, XP002467526 *

Also Published As

Publication number Publication date
WO2008003053A2 (fr) 2008-01-03
EP2044432A4 (fr) 2014-12-31
EP2044432A2 (fr) 2009-04-08
US20080018898A1 (en) 2008-01-24
EP2044432B1 (fr) 2023-03-29
US7839507B2 (en) 2010-11-23

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