[go: up one dir, main page]

WO2008099898A1 - ジスルフィド結合導入トランスグルタミナーゼ - Google Patents

ジスルフィド結合導入トランスグルタミナーゼ Download PDF

Info

Publication number
WO2008099898A1
WO2008099898A1 PCT/JP2008/052470 JP2008052470W WO2008099898A1 WO 2008099898 A1 WO2008099898 A1 WO 2008099898A1 JP 2008052470 W JP2008052470 W JP 2008052470W WO 2008099898 A1 WO2008099898 A1 WO 2008099898A1
Authority
WO
WIPO (PCT)
Prior art keywords
transglutaminase
disulfide bond
bond introduced
bond
mutation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/052470
Other languages
English (en)
French (fr)
Inventor
Keiichi Yokoyama
Mototaka Suzuki
Tatsuki Kashiwagi
Eiichiro Suzuki
Masayo Date
Seiichi Taguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Priority to JP2008558137A priority Critical patent/JPWO2008099898A1/ja
Priority to NZ579599A priority patent/NZ579599A/en
Priority to KR1020097019138A priority patent/KR101162049B1/ko
Priority to CN2008800052009A priority patent/CN101631860B/zh
Priority to EP20080711306 priority patent/EP2123756B1/en
Priority to AU2008215422A priority patent/AU2008215422B2/en
Publication of WO2008099898A1 publication Critical patent/WO2008099898A1/ja
Priority to US12/541,220 priority patent/US9062291B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　ＷＴトランスグルタミナーゼに、ジスルフィド結合（SS結合）を形成し得るシステイン残基への変異を導入することによって得られる、耐熱性及び／又はｐＨ安定性が向上したトランスグルタミナーゼ変異体タンパク質。
PCT/JP2008/052470 2007-02-15 2008-02-14 ジスルフィド結合導入トランスグルタミナーゼ Ceased WO2008099898A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2008558137A JPWO2008099898A1 (ja) 2007-02-15 2008-02-14 ジスルフィド結合導入トランスグルタミナーゼ
NZ579599A NZ579599A (en) 2007-02-15 2008-02-14 Transglutaminase having disulfide bond introduced therein
KR1020097019138A KR101162049B1 (ko) 2007-02-15 2008-02-14 디설파이드 결합 도입 트랜스글루타미나제
CN2008800052009A CN101631860B (zh) 2007-02-15 2008-02-14 引入二硫键的转谷氨酰胺酶
EP20080711306 EP2123756B1 (en) 2007-02-15 2008-02-14 Transglutaminase having disulfide bond introduced therein
AU2008215422A AU2008215422B2 (en) 2007-02-15 2008-02-14 Transglutaminase having disulfide bond introduced therein
US12/541,220 US9062291B2 (en) 2007-02-15 2009-08-14 Transglutaminase having disulfide bond introduced therein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-034916 2007-02-15
JP2007034916 2007-02-15

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/541,220 Continuation US9062291B2 (en) 2007-02-15 2009-08-14 Transglutaminase having disulfide bond introduced therein

Publications (1)

Publication Number Publication Date
WO2008099898A1 true WO2008099898A1 (ja) 2008-08-21

Family

ID=39690125

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/052470 Ceased WO2008099898A1 (ja) 2007-02-15 2008-02-14 ジスルフィド結合導入トランスグルタミナーゼ

Country Status (8)

Country Link
US (1) US9062291B2 (ja)
EP (1) EP2123756B1 (ja)
JP (1) JPWO2008099898A1 (ja)
KR (1) KR101162049B1 (ja)
CN (1) CN101631860B (ja)
AU (1) AU2008215422B2 (ja)
NZ (1) NZ579599A (ja)
WO (1) WO2008099898A1 (ja)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066785A1 (ja) 2007-11-19 2009-05-28 Ajinomoto Co., Inc. 繊維加工物及びその製造法
WO2010101256A1 (ja) 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
JP2017519045A (ja) * 2014-06-12 2017-07-13 シー・エス・ピー・シー ドフェン コーポレーションＣｓｐｃ Ｄｏｐｈｅｎ Ｃｏｒｐｏｒａｔｉｏｎ 酵素的方法による均一抗体薬物コンジュゲート
CN112980759A (zh) * 2021-03-08 2021-06-18 上海交通大学 增强Subtilisin基因转录水平以提高TG酶发酵水平的方法
WO2021177392A1 (ja) 2020-03-04 2021-09-10 味の素株式会社 変異型トランスグルタミナーゼ
WO2022071061A1 (ja) 2020-09-29 2022-04-07 味の素株式会社 変異型トランスグルタミナーゼ
CN115298306A (zh) * 2020-03-13 2022-11-04 居里公司 转谷氨酰胺酶变体

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5532928B2 (ja) * 2008-01-18 2014-06-25 味の素株式会社 セリン誘導体の製造方法及びこれに用いるタンパク質
WO2010005099A1 (ja) * 2008-07-09 2010-01-14 味の素株式会社 アミノヒドロキシ安息香酸類の製造方法
DE102010062600A1 (de) 2010-12-08 2012-06-14 Evonik Goldschmidt Gmbh Hydrophobisiertes Proteinhydrolysat
CN107365388B (zh) 2011-06-23 2021-05-14 味之素株式会社 多聚体蛋白的制备方法
CN104379742A (zh) 2012-05-29 2015-02-25 味之素株式会社 生产3-乙酰氨基-4-羟基苯甲酸的方法
AU2018337671B2 (en) 2017-09-19 2021-03-04 Paul Scherrer Institut Transglutaminase conjugation method and linker
US20200370026A1 (en) * 2017-11-30 2020-11-26 Amano Enzyme Inc. Modified transglutaminase
EP3941526A1 (en) 2019-03-19 2022-01-26 Paul Scherrer Institut Transglutaminase conjugation method with a glycine based linker
KR20230069211A (ko) 2020-09-18 2023-05-18 아라리스 바이오테크 아게 아미노산-기반 링커를 사용한 트란스글루타미나제 접합 방법
PE20231512A1 (es) 2020-10-25 2023-09-26 Araris Biotech Ag Medios y metodos para producir conjugados de ligador-anticuerpo
CN118159298A (zh) 2021-10-25 2024-06-07 阿拉里斯生物技术股份公司 生产抗体-接头偶联物的方法
CN118742328A (zh) 2022-02-22 2024-10-01 阿拉里斯生物技术股份公司 包含两个或更多个有效载荷的肽接头
WO2025082990A1 (en) 2023-10-15 2025-04-24 Araris Biotech Ag Antibody-drug conjugates using two different types of topoisomerase i inhibitors
US20260014265A1 (en) 2024-07-10 2026-01-15 Araris Biotech Ag TRIPLE-PAYLOAD ANTIBODY-DRUG CONJUGATES (ADCs)

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427471A (en) 1987-03-04 1989-01-30 Ajinomoto Kk Novel enzyme and production of protein gelatinized product using said enzyme
JPH03213574A (ja) 1990-01-10 1991-09-18 Ajinomoto Co Inc 獣毛繊維の改質方法
EP0481504A1 (en) 1990-10-19 1992-04-22 Amano Pharmaceutical Co., Ltd. Recombinant transglutaminase
JPH05199883A (ja) 1990-10-19 1993-08-10 Amano Pharmaceut Co Ltd リコンビナントトランスグルタミナーゼ
JPH0630771A (ja) 1992-07-14 1994-02-08 Ajinomoto Co Inc バクテリアルトランスグルタミナーゼの製造法及び関連物
JPH10504721A (ja) 1994-08-26 1998-05-12 ノボ ノルディスク アクティーゼルスカブ 微生物のトランスグルタミナーゼ、それらの産生及び使用
WO2001023591A1 (en) 1999-09-30 2001-04-05 Ajinomoto Co., Inc. Process for producing transglutaminase
JP2001321192A (ja) 2000-05-15 2001-11-20 Ajinomoto Co Inc 構造解析用タンパク質試料の調製方法
JP2002253272A (ja) * 2000-08-17 2002-09-10 Ajinomoto Co Inc 微生物由来トランスグルタミナーゼの改変方法
WO2002081694A1 (fr) 2001-03-30 2002-10-17 Ajinomoto Co.,Inc. Procede de secretion et de production de proteine
US6849095B2 (en) 2000-09-01 2005-02-01 Trumpler Gmbh & Co. Chemische Fabrik Agent and method for enzymatically tanning skins
WO2005103278A1 (ja) 2004-04-20 2005-11-03 Ajinomoto Co., Inc. タンパク質の製造法
JP2007034916A (ja) 2005-07-29 2007-02-08 Toshiba Tec Corp 商品販売登録データ処理装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
EP1310560A4 (en) * 2000-08-17 2005-07-13 Ajinomoto Kk METHOD FOR MODIFICATION OF TRANSGLUTAMINASE OF MICROORGANISMS (MTG)
KR20050106502A (ko) 2003-03-07 2005-11-09 아지노모토 가부시키가이샤 미생물 트랜스글루타미나제의 제조방법
JP4923573B2 (ja) 2003-07-16 2012-04-25 味の素株式会社 変異型セリンアセチルトランスフェラーゼ及びｌ−システインの製造法

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6427471A (en) 1987-03-04 1989-01-30 Ajinomoto Kk Novel enzyme and production of protein gelatinized product using said enzyme
JPH03213574A (ja) 1990-01-10 1991-09-18 Ajinomoto Co Inc 獣毛繊維の改質方法
EP0481504A1 (en) 1990-10-19 1992-04-22 Amano Pharmaceutical Co., Ltd. Recombinant transglutaminase
JPH05199883A (ja) 1990-10-19 1993-08-10 Amano Pharmaceut Co Ltd リコンビナントトランスグルタミナーゼ
JPH0630771A (ja) 1992-07-14 1994-02-08 Ajinomoto Co Inc バクテリアルトランスグルタミナーゼの製造法及び関連物
JPH10504721A (ja) 1994-08-26 1998-05-12 ノボ ノルディスク アクティーゼルスカブ 微生物のトランスグルタミナーゼ、それらの産生及び使用
WO2001023591A1 (en) 1999-09-30 2001-04-05 Ajinomoto Co., Inc. Process for producing transglutaminase
JP2001321192A (ja) 2000-05-15 2001-11-20 Ajinomoto Co Inc 構造解析用タンパク質試料の調製方法
JP2002253272A (ja) * 2000-08-17 2002-09-10 Ajinomoto Co Inc 微生物由来トランスグルタミナーゼの改変方法
US6849095B2 (en) 2000-09-01 2005-02-01 Trumpler Gmbh & Co. Chemische Fabrik Agent and method for enzymatically tanning skins
WO2002081694A1 (fr) 2001-03-30 2002-10-17 Ajinomoto Co.,Inc. Procede de secretion et de production de proteine
WO2005103278A1 (ja) 2004-04-20 2005-11-03 Ajinomoto Co., Inc. タンパク質の製造法
JP2007034916A (ja) 2005-07-29 2007-02-08 Toshiba Tec Corp 商品販売登録データ処理装置

Non-Patent Citations (21)

* Cited by examiner, † Cited by third party
Title
A. ICHINOSE ET AL., BIOCHEMISTRY, vol. 25, 1986, pages 6900
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
APP. ENV. MICRO., vol. 69, 2003, pages 3011 - 3014
CLARKE J. ET AL.: "Engineered disulfide bonds as probes of the bonding pathway of barnase: Increasing the stability of proteins against the rate of denaturation", BIOCHEMISTRY, vol. 32, 1993, pages 4322 - 4329, XP008116664 *
FEMS MICROBIOL. LETT., vol. 53, 1989, pages 299 - 303
J. BIOL. CHEM. 25, vol. 273, no. 52, 1998, pages 34868 - 74
J. BIOL. CHEM., vol. 268, no. 16, 1993, pages 11565 - 11572
J.BIOL.CHEM., vol. 241, 1966, pages 5518 - 5525
K. IKURA ET AL., BIOCHEMISTRY, vol. 27, 1988, pages 2898
KARLIN ET AL., PROC. NATL. ACAD. SCI., vol. 90, 1993, pages 5873 - 5877
KASHIWAGI T. ET AL.: "Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense", J. BIOL. CHEM., vol. 277, no. 46, 2002, pages 44252 - 44260, XP002405972 *
LI Y. ET AL.: "Effect on thermostability and catalytic activity of introducing disulfide bonds nto Aspergillus awamori glucoamylase", PROTEIN ENG., vol. 11, no. 8, 1998, pages 661 - 667, XP008116663 *
M.A. PHILLIPS ET AL., PROC. NATL. ACAD. SCI., vol. 87, 1990, pages 9333
MATSUMURA M. ET AL.: "Substantial increase of protein stability by multiple disulphide bonds", NATURE, vol. 342, no. 6247, 1989, pages 291 - 293, XP002973649 *
MYERS; MILLER, CABIOS, vol. 4, 1988, pages 11 - 17
NEEDLEMAN ET AL., J. MOL. BIOL., vol. 48, 1970, pages 444 - 453
PEARSON ET AL., PROC. NATL. ACAD. SCI., vol. 85, 1988, pages 2444 - 2448
PERRY L.J. ET AL.: "Disulfide bond engineered into T4 lysozyme: Stabilization of the protein toward thermal inactivation", SCIENCE, vol. 226, no. 4674, 1984, pages 555 - 557, XP008116665 *
PROT. EXP. PURI., vol. 26, 2002, pages 329 - 335
See also references of EP2123756A4 *
T. KANAJI ET AL., JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, 1993, pages 11565

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066785A1 (ja) 2007-11-19 2009-05-28 Ajinomoto Co., Inc. 繊維加工物及びその製造法
WO2010101256A1 (ja) 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
CN102341497A (zh) * 2009-03-06 2012-02-01 味之素株式会社 放线菌来源的耐热性转谷氨酰胺酶
CN102341497B (zh) * 2009-03-06 2013-11-06 味之素株式会社 放线菌来源的耐热性转谷氨酰胺酶
US8580537B2 (en) 2009-03-06 2013-11-12 Ajinomoto Co., Inc. Thermotolerant transglutaminase originating in actinomyces
JP2017519045A (ja) * 2014-06-12 2017-07-13 シー・エス・ピー・シー ドフェン コーポレーションＣｓｐｃ Ｄｏｐｈｅｎ Ｃｏｒｐｏｒａｔｉｏｎ 酵素的方法による均一抗体薬物コンジュゲート
WO2021177392A1 (ja) 2020-03-04 2021-09-10 味の素株式会社 変異型トランスグルタミナーゼ
CN115298306A (zh) * 2020-03-13 2022-11-04 居里公司 转谷氨酰胺酶变体
WO2022071061A1 (ja) 2020-09-29 2022-04-07 味の素株式会社 変異型トランスグルタミナーゼ
JPWO2022071061A1 (ja) * 2020-09-29 2022-04-07
CN112980759A (zh) * 2021-03-08 2021-06-18 上海交通大学 增强Subtilisin基因转录水平以提高TG酶发酵水平的方法

Also Published As

Publication number Publication date
CN101631860A (zh) 2010-01-20
EP2123756A1 (en) 2009-11-25
EP2123756A4 (en) 2010-06-30
CN101631860B (zh) 2012-07-18
EP2123756B1 (en) 2014-06-04
NZ579599A (en) 2012-03-30
AU2008215422A1 (en) 2008-08-21
KR101162049B1 (ko) 2012-07-04
KR20090120485A (ko) 2009-11-24
US20100143970A1 (en) 2010-06-10
AU2008215422B2 (en) 2011-07-14
JPWO2008099898A1 (ja) 2010-05-27
US9062291B2 (en) 2015-06-23

Similar Documents

Publication Publication Date Title
WO2008099898A1 (ja) ジスルフィド結合導入トランスグルタミナーゼ
AU2008301614A8 (en) Mutant pyrrolysyl-tRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
WO2009009086A3 (en) Methods and compositions for inactivating alpha 1,6 fucosyltransferase (fut 8) gene expression
WO2006081583A3 (en) Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
WO2008152140A3 (en) Recombinant transferrin mutants
PT1969007E (pt) Composições e métodos para a produção de uma composição
GB0524066D0 (en) 741 ii
WO2006034410A3 (en) Adding photoregulated amino acids to the genetic code
NZ595500A (en) Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
WO2010053518A3 (en) Methods and compositions for inactivating glutamine synthetase gene expression
WO2008070082A3 (en) Stem-progenitor cell specific micro-ribonucleic acids and uses thereof
WO2007130453A3 (en) Non-natural amino acid substituted polypeptides
DK2102337T3 (da) Microorganisme af slægten Corynebacterium med forhøjet L-lysinproduktionsevne samt fremgangsmåde til fremstilling af L-lysin under anvendelse af denne
WO2008036688A3 (en) Optimized antibodies that target hm1.24
WO2008054821A3 (en) Mutant hydrolase proteins with enhanced kinetics and functional expression
BR0316927A (pt) Proteìna, métodos para produzir um derivado de ácido glutâmico oticamente ativo e para produzir uma proteìna tendo uma atividade de d-aminotransferase, dna, célula
EP2599869A3 (en) Plants having enhanced yield-related traits and a method for making the same
MX2010001856A (es) Proteina.
WO2007066232A3 (en) Salts of fatty acids and methods of making and using thereof
WO2008146745A1 (ja) 新規なリゾホスファチジン酸アシル基転移酵素遺伝子
NO20080580L (no) Serumfritt kulturmedium for produksjon av redkombinante gonadotropiner
WO2008046810A3 (de) Allele des rel-gens aus coryneformen bakterien
PT2135949E (pt) Ácidos nucleicos que se ligam à mcp-1
WO2008085900A3 (en) Methods for generating novel stabilized proteins
WO2009010251A3 (en) Mutant dna polymerases and related methods

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200880005200.9

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08711306

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2008558137

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008711306

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2008215422

Country of ref document: AU

Ref document number: 579599

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 1020097019138

Country of ref document: KR

ENP Entry into the national phase

Ref document number: 2008215422

Country of ref document: AU

Date of ref document: 20080214

Kind code of ref document: A