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WO2007048379A1 - Element de couplage optique pour cellule a ecoulement - Google Patents

Element de couplage optique pour cellule a ecoulement Download PDF

Info

Publication number
WO2007048379A1
WO2007048379A1 PCT/DE2006/001806 DE2006001806W WO2007048379A1 WO 2007048379 A1 WO2007048379 A1 WO 2007048379A1 DE 2006001806 W DE2006001806 W DE 2006001806W WO 2007048379 A1 WO2007048379 A1 WO 2007048379A1
Authority
WO
WIPO (PCT)
Prior art keywords
coupling element
optical coupling
cell
flow
lens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2006/001806
Other languages
German (de)
English (en)
Inventor
Thuro Arnold
Kay Grossmann
Evelyn KRAWCZYK-BÄRSCH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum Dresden Rossendorf eV
Original Assignee
Helmholtz Zentrum Dresden Rossendorf eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helmholtz Zentrum Dresden Rossendorf eV filed Critical Helmholtz Zentrum Dresden Rossendorf eV
Publication of WO2007048379A1 publication Critical patent/WO2007048379A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/0006—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 with means to keep optical surfaces clean, e.g. by preventing or removing dirt, stains, contamination, condensation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B13/00—Optical objectives specially designed for the purposes specified below
    • G02B13/24—Optical objectives specially designed for the purposes specified below for reproducing or copying at short object distances

Definitions

  • the invention relates to an optical coupling element which serves as a connecting piece between a flow cell and a microscopic evaluation device.
  • An essential field of application of this optical coupling element is light and fluorescence microscopy for the examination of samples.
  • these microorganisms or microbial aggregates are characterized microscopically in situ (Strathmann.M., Wingender.J., Flemming, H.-C, Application of fluorescently labeled lectins for the visualization and biochemical characterization of polysaccharides in Biofilms of Pseudomonas aeruginosa, Journal of Microbiological Methods 50, 237-248, 2002).
  • the object of the invention is to develop an optical coupling element for the microscopic characterization of samples in flow cells in order to avoid a direct contact of the lens with the flow solution and thereby to keep the losses in the numerical aperture of the lens as low as possible.
  • the invention replaceable optical coupling element leads to a protection of the lens. Due to the optical coupling element, an additional image plane is laid at the location of the original object plane so as not to have to accept any aperture deterioration. Another possible use of this coupling element is given in the coupling of cryogenic cells in the microscopic beam path.
  • the sample carrier 2 is provided with a micrometer screw 10, via which the table can be moved freely in the Z direction.
  • the optical coupling element is not designed to be interchangeable.
  • an additional opening 12 is provided in the cell 1, via which the desired gas atmosphere is introduced into the cell 1.
  • the gas is pressed through a filter 13 into the cell 1.
  • An advantageous embodiment of the invention is to separate the second converging lens 8 by a transmitter liquid 14 from the nutrient liquid or the markers 15 in order to avoid contamination of the surface of the second converging lens 8.
  • This transmitter liquid 14 is introduced if necessary via a channel 9 in the lid 5.
  • a transmitter fluid 14 are suitable polar liquids that do not dissolve in water and form a meniscus 16 with water-like solutions due to the resulting surface tension.
  • Fig. 2 shows the optical path between the objective 11 and the sample 3.
  • a total of 3 (or 4 when using a transmitter liquid 14, [s Fig. 3]) additional optical active surfaces are inserted to the object (the sample 3) in to image the image plane 18 of the microscope. These are the two lenses and the spaces between them. Between the lens 7 and the lens 11, an additional image plane 19 is generated by the optical coupling element 7,8.
  • Other optically active surfaces can be introduced if color correction is necessary.
  • FIG. 3 shows the formation of a meniscus 16 between the polar transmitter liquid 14 and the bipolar nutrient medium 15.

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'objectif de l'invention est de mettre au point un élément de couplage optique pour la caractérisation microscopique d'échantillons dans des cellules à écoulement, cet élément permettant d'éviter un contact direct de l'objectif avec la solution d'écoulement et de réduire ainsi au maximum les pertes au niveau de l'ouverture numérique de l'objectif. L'élément de couplage optique selon l'invention comprend une cellule (1), un ou plusieurs porte-échantillons (2) situés à l'intérieur de cette cellule (1), des amenées et des évacuations (4) pour garantir un flux continu de différentes solutions à travers la cellule (1), ainsi que des ouvertures (12) pour introduire une atmosphère de gaz inerte. L'invention se caractérise en ce qu'un évidement est ménagé dans la partie supérieure (5) pour l'examen microscopique des échantillons (3) in situ, en ce qu'un élément de couplage optique additionnel (7, 8) est placé dans l'évidement de la partie supérieure (5) et en ce que le système optique est constitué de deux milieux optiquement actifs qui sont disposés à l'opposé optiquement l'un vis-à-vis de l'autre.
PCT/DE2006/001806 2005-10-27 2006-10-19 Element de couplage optique pour cellule a ecoulement Ceased WO2007048379A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005051406A DE102005051406A1 (de) 2005-10-27 2005-10-27 Optisches Koppelelement für eine Durchflusszelle
DE102005051406.5 2005-10-27

Publications (1)

Publication Number Publication Date
WO2007048379A1 true WO2007048379A1 (fr) 2007-05-03

Family

ID=37733712

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2006/001806 Ceased WO2007048379A1 (fr) 2005-10-27 2006-10-19 Element de couplage optique pour cellule a ecoulement

Country Status (2)

Country Link
DE (1) DE102005051406A1 (fr)
WO (1) WO2007048379A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734372A (en) * 1983-02-04 1988-03-29 Brown University Research Foundation Cell culturing methods and apparatus
DE9101082U1 (de) * 1990-02-05 1991-04-18 Kempe, Eberhard, Dipl.-Ing., 1000 Berlin Einrichtung zur Überprüfung des morphologischen Zustandes von Biomassen in Fermentern
DE4032002A1 (de) * 1989-10-11 1991-06-06 Abb Patent Gmbh In situ mikroskopsonde und messverfahren
DE4443902C1 (de) * 1994-12-01 1996-04-18 Will Prof Dr Minuth Kammer zur Kultivierung von Zellen, insbesondere Mikroskopkammer
WO1999019752A1 (fr) * 1997-10-09 1999-04-22 Imagyn Medical Technologies, Inc. Systeme d'objectif au saphir

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD51714A1 (de) * 1965-10-16 1966-12-05 Annelies Heymer Vorrichtung zur mikroskopischen Untersuchung unter hohem Druck bei tiefen Temperaturen
GB9314926D0 (en) * 1993-07-19 1993-09-01 Optiglass Ltd Spectrophotometer cell
AT407012B (de) * 1999-04-29 2000-11-27 Werner Dipl Ing Dr Tech Juette Verfahren zur behandlung von oberflächen sowie vorrichtung zur durchführung dieses verfahrens

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734372A (en) * 1983-02-04 1988-03-29 Brown University Research Foundation Cell culturing methods and apparatus
DE4032002A1 (de) * 1989-10-11 1991-06-06 Abb Patent Gmbh In situ mikroskopsonde und messverfahren
DE9101082U1 (de) * 1990-02-05 1991-04-18 Kempe, Eberhard, Dipl.-Ing., 1000 Berlin Einrichtung zur Überprüfung des morphologischen Zustandes von Biomassen in Fermentern
DE4443902C1 (de) * 1994-12-01 1996-04-18 Will Prof Dr Minuth Kammer zur Kultivierung von Zellen, insbesondere Mikroskopkammer
WO1999019752A1 (fr) * 1997-10-09 1999-04-22 Imagyn Medical Technologies, Inc. Systeme d'objectif au saphir

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HASHIZUME K ET AL: "Individual mesoscopic structures studied with sub-micrometer optical detection techniques: CdSe nanocrystals capped with TOPO and ZnS-overcoated system", JOURNAL OF LUMINESCENCE, AMSTERDAM, NL, vol. 98, no. 1-4, July 2002 (2002-07-01), pages 49 - 56, XP004368922, ISSN: 0022-2313 *

Also Published As

Publication number Publication date
DE102005051406A1 (de) 2007-05-03

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Legal Events

Date Code Title Description
DPE2 Request for preliminary examination filed before expiration of 19th month from priority date (pct application filed from 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase

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