WO2004085420A1 - Bicyclic compounds useful as angiotensin ii agonists - Google Patents

Abstract

There is provided compounds of formula I, wherein R1a, R1b, X, Y1, Y2, Y3, Y4, Z 1, Z2, R2 and R3 have meanings given in the description, and pharmaceutically-acceptable salts thereof, which compounds are useful as selective agonists of the AT2 receptor, and thus, in particular, in the treatment of inter alia gastrointestinal conditions, such as dyspepsia, IBS and MOF, and cardiovascular disorders.

Description

BICYCLIC COMPOUNDS USEFUL AS ANGIOTENSIN II AGONISTS

Field of the Invention

This invention relates to novel pharmaceutically -useful compounds, in particular compounds that are angiotensin II (Angll) agonists, more particularly agonists of the Angll type 2 receptor (hereinafter the AT2 receptor), and especially agonists that bind selectively to that receptor. The invention further relates to the use of such compounds as medicaments, to pharmaceutical compositions containing them, and to synthetic routes to their production.

Background and Prior Art

The endogenous hormone Angll is a linear octapeptide (Asp -Arg -Val -

Tyr -lie -His -Pro -Phe ), and is the active component of the renm- angiotensin system (RAS). It is produced by the sequential processing of the pro-hormone angiotensinogen by renin and angiotensin converting enzyme (ACE).

The renin-angiotensin system (RAS) plays an important role in the regulation of blood pressure, body fluid and electrolyte homeostasis. Ang II exerts these physiological actions in many organs including the kidneys, the adrenal glands, the heart, blood vessels, the brain, the gastrointestinal tract and the reproductive organs (de Gasparo et al, Pharmacol. Rev. (2000) 52, 415-472).

Two main classes of Angll receptors have been identified, and designated as the type 1 receptor (hereinafter the ATI receptor) and the AT2 receptor. The ATI receptor is expressed in most organs, and is believed to be responsible for the majority of the biological effects of Angll. The AT2 receptor is more prevalent than the ATI receptor in fetal tissues, the adult ovaries, the adrenal medulla and the pancreas. An equal distribution is reported in the brain and uterus (Ardaillou, J. Am. Soc. NephroL, 10, S30-39 (1999)).

Several studies in adult individuals appear to demonstrate that, in the modulation of the response following Angll stimulation, activation of the AT2 receptor has opposing effects to those mediated by the ATI receptor.

The AT2 receptor has also been shown to be involved in apoptosis and inhibition of cell proliferation (see de Gasparo et al, supra). Further, it seems to play a role in blood pressure control. For example, it has been shown in transgenic mice lacking AT2 receptors that their blood pressure was elevated. Furthermore, it has been concluded that the AT2 receptor is involved in exploratory behaviour, pain sensitivity and thermoregulation.

The expression of AT2 receptors has also been shown to increase during pathological circumstances, such as vascular injury, wound healing and heart failure (see de Gasparo et al, supra).

The expected pharmacological effects of agonism of the AT2 receptor are described generally in de Gasparo et al, supra.

More recently, AT2 receptor agonists have been shown to be of potential utility in the treatment and/or prophylaxis of disorders of the alimentary tract, such as dyspepsia and irritable bowel syndrome, as well as multiple organ failure (see international patent application WO 99/43339). International patent application WO 00/68226 and US patent number 6,235,766 disclose compounds comprising substituted imidazolyl groups, which groups are attached, via a methylene bridge, to a phenylthiophene moiety, as agonists of angiotensin-(l-7) receptors. International patent application WO 02/072569 discloses similar compounds as agonists of the same receptors. International patent application WO 01/44239 discloses biphenylsulfonamide compounds as combined angiotensin and endothelin receptor antagonists. The use of the compounds as Ang II receptor agonists is neither mentioned nor suggested in any of these documents.

Peptide and non-peptide AT2 receptor agonists, unrelated structurally to those described herein, and potential uses thereof, have been disclosed in, for example, international patent applications WO 00/38676, WO 00/56345, WO 00/09144, WO 99/58140, WO 99/52540, WO 99/46285, WO 99/45945, WO 99/42122, WO 99/40107, WO 99/40106, WO 99/39743, WO 99/26644, WO 98/33813, WO 00/02905 and WO 99/46285; US patent number 5,834,432; and Japanese patent application JP 143695.

Angll antagonists (which bind to the ATI and/or AT2 receptors) have been disclosed in inter alia European patent application EP 512 675; international patent applications WO 94/27597, WO 94/02142, WO 95/23792 and WO 94/03435; and US patent numbers 5,091,390, 5,177,074, 5,412,097, 5,250,521, 5,260,285, 5,376,666, 5,252,574, 5,312,820, 5,330,987, 5,166,206, 5,932,575, 5,444,068, 5,635,525, 5,541,229, 5,864,043 and 5,240,928. Angll agonists, and particularly AT2 receptor agonists, are not contemplated in any of these documents.

US 5,312,820 discloses N-carbamoyl and N-oxycarbonyl derivatives of biphenylmethylamine-based Angll antagonists. The use of these compounds as Angll agonists, and particularly AT2 receptor agonists, is not contemplated.

In US 5,240,928 and US 5,330,987, N-cyanophenyl- and N- cyanoheteroaryl-N-carbonyl derivatives of biphenylmethylamines are disclosed generically as potential intermediates useful in the synthesis of inter alia quinazolinone- and pyrimidinone-containing Ang II antagonists. The use of these intermediates as pharmaceuticals is neither mentioned nor suggested.

US patent number 5,444,067 discloses compounds comprising a 5,7- dimethyl-2-ethylpyridinoimidazolyl group attached, via a methylene bridge, to a phenylthiophene moiety, as Angll agonists. Further, international patent application WO 02/96883 discloses compounds comprising certain monocyclic heterocyclic groups attached, via a methylene bridge, to substituted phenylthiophene and biphenyl moieties. The compounds disclosed therein are indicated as Angll agonists and in particular as selective AT2 receptor agonists.

However, there remains a need for effective and/or selective AT2 receptor agonists, which are expected to find utility in inter alia the above- mentioned conditions.

Disclosure of the Invention

According to the invention there is provided compounds of formula I,

wherein

X represents -0-, -C(O)- or -S(0)₂-;

R , 1a and R , 1b independently represent H, C_1-6 alkyl, C_1-6 alkoxy-C_1-6 alkyl, Ar¹, Het¹, Cι_₃ alkyl-Ar², C_1-3 alkyl-Het², C_1-3 alkoxy-Ar³ or C_1-3 alkoxy- Het³; or, in the case where X represents -C(O)- , R^la may also represent C_1- alkoxy or -O-Ar⁴;

Ar , Ar², Ar³ and Ar each independently represent a C_6-10 aryl group, which group is optionally substituted by one or more substituents selected from =0, -OH, cyano, halo, nitro, C_1-6 alkyl (optionally terminated by -N(H)C(0)OR^lla), Cι_-6 alkoxy, phenyl, -N(R^12a)R^12b, -C(0)R^12c, -C(0)OR^12d, -C(0)N(R^12e)R^12f, -N(R^12g)C(0)R^12h, -N(R¹²ⁱ)C(0)N(R^12j)R^12k, -N(R^12m)S(0)₂R^{l lb},-S(0)_nR^llc, -OS(0)₂R^{l ld} and -S(0)₂N(R¹²ⁿ)R^12p; Het , Het and Het each independently represent a four- to twelve- membered heterocyclic group containing one or more heteroatoms selected from oxygen, nitrogen and/or sulfur, which heterocyclic group is optionally substituted by one or more substituents selected from =0, -OH, cyano, halo, nitro, C_1-6 alkyl (optionally terminated by -N(H)C(0)OR^{l la}), Ci_₆ alkoxy, phenyl, -N(R^12a)R^12b, -C(0)R^12c, -C(0)OR^12d, -C(0)N(R^12e)R^12f, -N(R¹² )C(0)R^12h, -N(R¹²ⁱ)C(0)N(R^12j)R¹² , -N(R^12m)S(0)₂R^llb, -S(0)_nR^llc, -OS(0)₂R^lld and -S(0)₂N(R¹²ⁿ)R^12p; R^lla to R^lld independently represent C_1-6 alkyl; R^12a to R^12p independently represent H or Cι_-6 alkyl; n represents 0, 1 or 2;

Yi, Y₂, Y₃ and Y independently represent -CH- or -CF-; Zi represents -CH-, -0-, -S-, -N- or -CH=CH-; Z₂ represents -CH-, -0-, -S- or -N-; provided that:

(a) ∑ι and Z₂ are not the same;

(b) when Zi represents -CH=CH-, then Z₂ may only represent -CH- or -N-; and

(c) other than in the specific case in which Zi represents — CH=CH-, and Z₂ represents -CH-, when one of Zi and Z₂ represents -CH-, then the other represents -O- or -S-;

R² represents -S(0)₂N(H)C(0)R⁴, -S(0)₂N(H)S(0)₂R⁴, -C(0)N(H)S(0)₂R⁴, or, when Z_\ represents -CH=CH-, R may represent

-N(H)S(0)₂N(H)C(0)R⁵ or -N(H)C(0)N(H)S(0)₂R⁵; R represents Cι_₆ alkyl, C_1-6 alkoxy or C_1-6 alkoxy-Cι_-6-alkyl;

R⁴ represents C_1-6 alkyl, Cι.₆ alkoxy, Cι_₆ alkoxy-Cι_₆-alkyl, Cι_ alkylamino or di-C_1- alkylamino; and

R⁵ represents C_1-6 alkyl, or a pharmaceutically-acceptable salt thereof, which compounds and salts are referred to together hereinafter as "the compounds of the invention".

Pharmaceutically-acceptable salts include acid addition salts and base addition salts. Such salts may be formed by conventional means, for example by reaction of a free acid or a free base form of a compound of the invention with one or more equivalents of an appropriate acid or base, optionally in a solvent, or in a medium in which the salt is insoluble, followed by removal of said solvent, or said medium, using standard techniques (e.g. in vacuo or by freeze-drying). Salts may also be prepared by exchanging a counter-ion of a compound of the invention in the form of a salt with another counter-ion, for example using a suitable ion exchange resin.

Unless otherwise specified, alkyl groups, and the alkyl parts of alkoxy, alkoxyalkyl, alkylamino, alkyl-aryl, alkyl-heterocyclic groups, alkoxy-aryl and alkoxy-heterocyclic groups, as defined herein may be straight-chain or, when there is a sufficient number (i.e. a minimum of two or three, as appropriate) of carbon atoms, be branched-chain, and/or cyclic. Further, when there is a sufficient number (i.e. a minimum of four) of carbon atoms, such groups may also be part cyclic/acyclic. Such alkyl groups, and alkyl parts of alkoxy, alkoxyalkyl, alkylamino, alkyl-aryl, alkyl-heterocyclic, alkoxy-aryl and alkoxy-heterocyclic groups may also be saturated or, when there is a sufficient number (i.e. a minimum of two) of carbon atoms, be unsaturated. Unless otherwise specified, such groups may also be substituted by one or more halo, and especially fluoro, atoms.

For the avoidance of doubt, alkoxy and aryloxy (e.g. -O-Ar ) groups are attached to the rest of the molecule via the oxygen atom in that group, alkylamino groups are attached to the rest of the molecule via the nitrogen atom of the amino part of that group, alkoxyalkyl, alkyl-aryl and alkyl- heterocyclic groups are attached to the rest of the molecule via the alkyl part of that group, and alkoxy-aryl and alkoxy-heterocyclic groups are attached to the rest of the molecule via the alkyl part of the alkoxy part of that group.

The term "halo", when used herein, includes fluoro, chloro, bromo and iodo.

For the avoidance of doubt, in cases in which the identity of two or more substituents in a compound of the invention (for example R^la and R^lb) may be the same, the actual identities of the respective substituents are not in any way interdependent. For example, in the situation in which R^la and R¹ both represent Cι_-6 alkyl groups, the two alkyl groups in question may be the same of different. Similarly, when aryl and heterocyclic groups are substituted by more than one substituent as defined herein, the identities of the individual substituents are not to be regarded as being interdependent.

Cβ-io ^aryl groups include phenyl, naphthyl and the like (preferably phenyl). Preferred optional substituents on aromatic groups include C_1-3 alkyl groups (such as methyl) or C_1-3 alkoxy groups.

Het (Het to Het ) groups that may be mentioned include those containing 1 to 4 heteroatoms (selected from the group oxygen, nitrogen and/or sulfur) and in which the total number of atoms in the ring system are between five and twelve. Het (Het to Het ) groups may be fully saturated, wholly aromatic, partly aromatic and/or bicyclic in character. Heterocyclic groups that may be mentioned include benzodioxanyl, benzodioxepanyl, benzodioxolyl, benzofuranyl, benzofurazanyl, benzimidazolyl, benzomorpholinyl, benzothiophenyl, chromanyl, cinnolinyl, dioxanyl, furanyl, hydantoinyl, imidazolyl, imidazo[l,2-_.]pyridinyl, indolyl, isoquinolinyl, isoxazolyl, maleimido, morpholinyl, oxazolyl, phthalazinyl, piperazinyl, piperidinyl, purinyl, pyranyl, pyrazinyl, pyrazolyl, pyridinyl, pyrimindinyl, pyrrolidinonyl, pyrrolidinyl, pyrrolinyl, pyrrolyl, quinazolinyl, quinolinyl, 3-sulfolenyl, tetrahydropyranyl, tetrahydrofuranyl, thiazolyl, thiophenyl, thiochromanyl, triazolyl, tetrazolyl and the like. Values of Het¹ that may be mentioned include thiophenyl, furanyl, pyridinyl and thiazolyl. Values of Het² that may be mentioned include pyridinyl, furanyl, thiophenyl and thiazolyl. Values of Het³ that may be mentioned include pyridinyl.

Substituents on Het (Het¹ to Het³) groups may, where appropriate, be located on any atom in the ring system including a heteroatom. The point of attachment of Het (Het to Het ) groups may be via any atom in the ring system including (where appropriate) a heteroatom, or an atom on any fused carbocyclic ring that may be present as part of the ring system. Het (Het¹ to Het³) groups may also be in the N- or _?-oxidised fonn.

Preferred ring systems comprising the substituents Y_ls Y₂, Y₃ and Y₄ include phenyl groups. For the avoidance of doubt, the ring systems in compounds of formula I that comprise the groups Zi and Z₂, are aromatic in nature. In some instances, for example in cases where one or more of Z_\ and Z₂ represent -CH- or -N- the skilled person will appreciate that an additional H atom may necessarily be bonded to that CH group or N atom, in order to ensure that the rules of valency are adhered to. Preferred ring systems comprising Zi and Z₂ include oxazole groups, thiazole groups, phenyl groups, pyridinyl groups, thiophenyl groups and furanyl groups.

In this respect, compounds of the invention may exhibit tautomerism. All tautomeric forms and mixtures thereof are included within the scope of the invention.

Compounds of the invention also contain one or more asymmetric carbon atoms and may therefore exhibit optical and/or diastereoisomerism. Diastereoisomers may be separated using conventional techniques, e.g. chromatography or fractional crystallisation. The various stereoisomers may be isolated by separation of a racemic or other mixture of the compounds using conventional, e.g. fractional crystallisation or HPLC, techniques. Alternatively the desired optical isomers may be made by reaction of the appropriate optically active starting materials under conditions which will not cause racemisation or epimerisation, or by derivatisation, for example with a homochiral acid followed by separation of the diastereomeric derivatives by conventional means (e.g. HPLC, chromatography over silica). All stereoisomers are included within the scope of the invention.

Preferred compounds of the invention include those in which R^la and R^lb independently represent H, C_1-6 alkyl, C_1-6 alkoxy-C_1-6 alkyl, Ar¹, Het¹, C_1-3 alkyl- Ar², C_1-3 alkyl-Het², Cι_-3 alkoxy-Ar³ or C_1-3 alkoxy-Het³.

It is further preferred that, when X represents -C(O)-, R^la does not represent

-O-Ar⁴

More preferred compounds of the invention include those in which:

R^la represents H, C_1-5 alkoxy, or, more preferably, Cι_₅ alkyl, Ar¹ or Het¹;

Het¹ represents thiophenyl;

R represents H or, more preferably, C_1- alkyl, phenyl (optionally substituted by one or more .₂ alkyl groups), C_1-2 alkylphenyl, Cι_₂ alkyl-

Het² represents pyridinyl.

More preferred compounds of the invention include those in which: X represents -O- or, more preferably, -C(O)-;

R ^a represents H, C_1-3 alkoxy (e.g. ethoxy), or, more preferably, C_1-4 alkyl (e.g. methyl or n-butyl), phenyl or thiophenyl;

R represents H or, more preferably, Cj_-3 alkyl (e.g. methyl or ethyl), phenyl (optionally substituted by one or more methyl groups), benzyl or methylpyridinyl;

Yi, Y₂, Y₃ and Y₄ all represent -CH-;

Zi represents -CH=CH- or, more preferably, -S-;

Z₂ represents -CH-;

R² represents -S(0)₂N(H)C(0)R⁴; R represents n-butyl or, particularly, iso-butyl; R represents n-butyl, n-butoxymethyl, .sobutoxy and especially, w-butoxy.

When X represents -0-, preferred values of R^la include Cι_₃ alkyl, such as methyl. When X represents -C(O)-, preferred values of R^la include H, C_1-3 alkoxy (e.g. ethoxy), Cι_₄ alkyl (e.g. methyl or /.-butyl), phenyl or thiophenyl. When X represents -S(0)₂-, preferced values of R^la include C_1-3 alkyl, such as methyl.

When R² represents -S(0)₂N(H)C(0)R⁴, preferred values of R⁴ include n- butoxymethyl, wo-butoxy and especially, n-butoxy.

It is preferred that, when R represents Ar or Het , then those groups are not substituted with a cyano group, e.g. at the 2-position relative to the point

11. 1 1 of attachment. Further, when R represents Ar or Het , and Zi represents -CH=CH-, it is preferred that Z₂ does not represent -CH-.

More preferred compounds of the invention include the compounds of the examples described hereinafter.

Compounds of formula I may be made in accordance with techniques well known to those skilled in the art, for example as described hereinafter.

According to a further aspect of the invention there is provided a process for the preparation of a compound of formula I, which process comprises:

(i) for compounds of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ or -S(0)₂N(H)S(0) R⁴, and R⁴ is as hereinbefore defined, reaction of a compound of formula II,

wherein R^la, R^lb, X, Y_ls Y₂, Y₃, Y₄, Z_l5 Z₂ and R³ are as hereinbefore defined with a compound of formula III,

R⁴GL III

wherein G represents C(O) or S(0)₂ (as appropriate), L¹ represents a suitable leaving group, such as halo (e.g. chloro or bromo) and R⁴ is as hereinbefore defined, for example at around room temperature or above (e.g. up to 60-70°C) in the presence of a suitable base (e.g. pyrollidinopyridine, pyridine, triethylamine, tributylamine, trimethylamine, dimethylaminopyridine, di-zso-propylamine, 1 ,8-diazabicyclo[5.4.0]undec- 7-ene, sodium hydroxide, or mixtures thereof) and an appropriate solvent (e.g. pyridine, dichloromethane, chloroform, tetrahydrofuran, dimethylformamide, trifluoromethylbenzene or triethylamine). Preferred base/solvent systems for compounds of formula III in which G is C(O) include pyrollidinopyridine/pyridine, pyrollidinopyridine/triethylamine, dimethylaminopyridine/pyridme or dimethylaminopyridine/triethylamine. Preferred base/solvent systems for compounds of formula III in which G is S(0)₂ include NaOH/THF; (ii) for compounds of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ and R⁴ represents _₆ alkoxy-C_1-6-alkyl, coupling of a compound of formula II as hereinbefore defined with a compound of formula IV,

R^4aC0₂H IV

wherein R^4a represents Cι_₆ alkoxy-Cι_₆-alkyl, for example under similar conditions to those described under process step (i) above, in the presence of a suitable coupling reagent (e.g. l, -carbonyl-diimidazole, N,N'- dicyclohexylcarbodiimide, N,N'-disuccinimidyl carbonate, benzotriazole-1- yloxytris(dimethylamino)phosρhoniumhexafluorophosphate, 2-( 1 H- benzotriazole- 1 -yl)- 1,1,3 ,3 -tetramethyluronium hexafluorophosphate, benzotriazole- 1 -yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate, bromo-tris-pynOlidinophosponium hexafluorophosphate or 2-(lH- benzotriazole-l-yl)-l,l,3,3-tetramethyluronium tetrafluorocarbonate), a suitable base (as mentioned in process step (i) above) and an appropriate solvent (as mentioned in process step (i) above);

(iii) for compounds of formula I in which R² represents -C(0)N(H)S(0)₂R⁴ and R⁴ is as hereinbefore defined, coupling of a compound of formula V,

wherein R^la, R^lb, X, Y_l5 Y₂, Y₃, Y₄, Z Z₂ and R³ are as hereinbefore defined with a compound of formula VI,

wherein R⁴ is as hereinbefore defined, for example in the presence of a suitable coupling reagent (such as those described in process step (ii) hereinbefore), and under similar reaction conditions to those described hereinbefore for preparation of compounds of formula I in which R⁴ represents C_1-6 alkoxy-Cι_₆-alkyl;

(iv) for compounds of formula I in which R² represents -C(0)N(H)S(0)₂R⁴ and R⁴ is as hereinbefore defined, coupling of a compound of formula VII,

wherein R ^a, R , X, Y_l5 Y₂, Y₃, Y , Z_ls Z₂ and R are as hereinbefore defined with a compound of formula VIII,

R⁴S(0)₂C1 VIII

wherein R⁴ is as hereinbefore defined, for example at around 50°C in the presence of a suitable base (e.g. sodium hydride) and an appropriate organic solvent (e.g. THF); (v) for compounds of formula I in which R² represents -N(H)S(0)₂N(H)C(0)R⁵ and R⁵ is as hereinbefore defined, reaction of a compound of fomiula IX,

wherein R^la, R^lb, X, Y_b Y₂, Y₃, Y₄, Z_l5 Z₂ and R³ are as hereinbefore defined with a compound of formula X,

R⁵C(0)N(H)S(0)₂C1 X

wherein R⁵ is as hereinbefore defined, for example at or around room temperature in the presence of a suitable base (e.g. sodium hydroxide or triethylamine) and a suitable organic solvent (e.g. benzene or dichloromethane) ;

(vi) for compounds of formula I in which R² represents -N(H)C(0)N(H)S(0)₂R⁵ and R⁵ is as hereinbefore defined, reaction of a compound of formula IX as hereinbefore defined with a compound of formula XI,

R⁵S(0)₂N(H)C(0)OR^x XI wherein R^x represents Cι_₂ alkyl and R is as hereinbefore defined, for example at or around room temperature in the presence of a suitable organic solvent (e.g. dichloromethane);

(vii) for compounds of formula I in which R² represents -N(H)C(0)N(H)S(0)₂R⁵ and R⁵ is as hereinbefore defined, reaction of a compound of fonnula IX as hereinbefore defined with an isocyanate compound of formula XII,

R⁵S(0)₂NCO XII

wherein R⁵ is as hereinbefore defined, for example at or around room temperature in the presence of a suitable organic solvent (e.g. dichloromethane) ;

(viii) for compounds of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ and R represents C_1-6 alkylamino, reaction of a compound of formula II as hereinbefore defined with an isocyanate compound of formula XIII,

R^4bNCO XIII

wherein R is C_1-6 alkyl, for example at or around room temperature in the presence of a suitable base (e.g. sodium hydroxide or potassium hydroxide and an appropriate organic solvent (e.g. acetone or acetonitrile);

(ix) for compounds of formula I in which R² represents -S(0)₂N(H)C(0)R and R represents di-C_1-6 alkylamino, reaction of a corresponding compound of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ and R⁴ represents C_1-6 alkoxy with an amine of formula Xllla,

R^4cN(H)R^4d Xllla

wherein R^4c and R^4d independently represent C_1-6 alkyl, for example at above room temperature (e.g. at between 70°C and 100°C) in the presence of an appropriate organic solvent (e.g. toluene); or

(x) for compounds of fonnula I in which X represents O, reductive amination of a compound of formula XIV,

9 wherein Y_ls Y₂, Y₃, Y₄, Zi, Z₂, R and R are as hereinbefore defined, in the presence of a compound of formula XV,

R^laONHR^lb XV

wherein R ^a and R¹ are as hereinbefore defined under standard conditions (e.g. in the presence of a suitable organic solvent (e.g. methanol, ethanol, dichloromethane, dichloroethane, tetrahydrofuran or dioxane), and, subsequently, an appropriate reducing agent (e.g. sodium borohydride, sodium cyanoborohydride or NaBH(OAc)₃)). Compounds of formula V may be prepared by oxidation of a compound of formula XVI,

wherein R^la, R^l , X, Y Y₂, Y₃, Y₄, Z_l5 Z₂ and R³ are as hereinbefore defined, for example under standard oxidation conditions in the presence of a suitable oxidising agent, such as potassium permanganate or chromium (VI) oxide.

Compounds of formulae II, VII, IX and XVI in which X represents -C(O)- or -S(0)₂- may be prepared by reaction of a compound of formula XVII,

wherein R^y represents -S0₂NH₂ (in the case of a compound of formula II), -CONH₂ (in the case of a compound of formula VII), -NH₂ (in the case of a compound of formula IX), or -CHO (in the case of a compound of formula XVI), and R^lb, Y Y₂, Y₃, Y₄, Z,, Z₂ and R³ are as hereinbefore defined, with a compound of formula XVIII,

R^X'L¹ XVIII

wherein X^a represents -C(O)- or -S(0)₂- and R^la and L are as hereinbefore defined, for example at or around room temperature in the presence of a suitable base (e.g. triethylamine, 4-dimethylaminopyridine, pyrollidinopyridine, diisopropylethylamine or mixtures thereof) and an appropriate organic solvent (e.g. dichloromethane, chloroform, tetrahydrofuran, dioxane or dimethylformamide). Alternatively, compounds of formulae II, VII, IX and XVI in which R^la represents H and X represents -C(O)- may be prepared in this way by reaction of a compound of formula XVII with ammonium formate, for example at above room temperature (e.g. between 80 to 120°C) in the presence of an appropriate organic solvent (e.g. acetonitrile, dioxane, dimethylformamide, ethylene glycol dimethyl ether, l-methyl-2-pyrrolidinone or dimethylsulphoxide). Preferably compounds of formula XVII are protected at the R^y position prior to carrying out the reaction with the compound of formula XVIII or ammonium formate. Suitable protecting groups for different values of R^y are described hereinafter. If a protected version of a compound of formula XVII is employed, this reaction may be followed by deprotection of the R^y group under standard conditions, for example as described hereinafter.

Compounds of formulae II, VII, IX or XVI in which X represents -C(O)- or — S(0)₂- may alternatively be prepared by reaction of a compound of formula XIX,

wherein L¹, Y_1} Y₂, Y₃, Y , Z_l5 Z₂, R³ and R^y are as hereinbefore defined (L¹ may, in particular, represent bromo), with a compound of formula XX,

wherein R^la, X^a and R¹ are as hereinbefore defined, for example at around or below room temperature in the presence of a suitable base (e.g. potassium hydroxide, potassium tert-butoxide, triethylamine or άi-iso- propylethylamine) and an appropriate organic solvent (e.g. DMSO, DMF, THF or CH₂C1₂). As with compounds of formula XVII, compounds of formula XIX are preferably protected at the R^y position prior to carrying out the reaction with the compound of formula XX. If a protected version of a compound of formula XIX is employed, this reaction may be followed by deprotection of the R^y group under standard conditions, for example as described hereinafter.

Compounds of formulae II, VII, IX and XVI may alternatively be prepared by reaction of a compound of formula XXI,

wherein L² represents a suitable leaving group, such as trimethylsulphonate, or halo, such as iodo or bromo, and R ^a, R , X, Yj, Y₂, Y₃ and Y₄ are as hereinbefore defined, with a compound of formula XXII,

wherein R^y, R³, Z¹ and Z² are as hereinbefore defined, or a protected derivative thereof, for example in the presence of an appropriate coupling catalyst system (e.g. a palladium catalyst, such as Pd(PPh₃)₄ or

Pd(OAc)₂/ligand (wherein the ligand may be, for example, PPh₃, P(o-Tol)₃ or l,l'-bis(diphenylphosphino) ferrocene)) and a suitable base (e.g. sodium hydroxide, sodium carbonate, potassium carbonate, cesium carbonate, cesium fluoride, triethylamine or di-ωo-propylamine)), as well as a suitable solvent system (e.g. toluene, ethanol, dimethoxymethane, dimethylformamide, ethylene glycol dimethyl ether, water, dioxane or mixtures thereof). This reaction may be carried out at above room temperature (e.g. at the reflux temperature of the solvent system that is employed).

Compounds of formulae II, VII, IX and XVI in which X represents O may alternatively be prepared by reaction of a compound of formula XXIII, XXIII

wherein Y_l5 Y₂, Y₃, Y₄, Z_\, Z₂, R³ and R^y are as hereinbefore defined, or an appropriate protected derivative thereof, with a compound of formula XV as hereinbefore defined, for example under conditions such as those described hereinbefore for preparation of compounds of formula I.

Compounds of formula XIV may be prepared by reaction of a compound of formula XXIV,

wherein L , Y_1? Y₂, Y₃ and Y₄ are as hereinbefore defined with a compound of formula XXV,

9 wherein R , R , Zi and Z₂ are as hereinbefore defined, for example under similar conditions to those decribed hereinbefore for preparation of compounds of formulae II, VII, IX and XVI (third process).

Compounds of formula XV are readily available. For example compounds of formula XV may be prepared by reaction of a compound of formula

XXVI,

R^l NH₂ XXVI

wherein R¹ is as hereinbefore defined, with an appropriate oxidising agent

(for example hydrogen peroxide or e/α-chloroperbenzoic acid), for example in the presence of a suitable solvent (such as ethanol of methanol),

1 . followed by reaction of the intermediate hydroxylamine (R N(H)OH) with a compound of formula XXVII,

R^laL^! XXVII

wherein L¹ and R^la are as hereinbefore defined, for example in the presence of a suitable base (e.g. sodium hydride, sodium bicarbonate, sodium hydroxide or triethylamine) and an appropriate organic solvent (e.g. dioxane, dichloromethane, dimethylformamide and/or acetone). Compounds of formula XV may alternatively be prepared by reaction of an alcohol of formula XXVIII,

R^laOH XXVIII

wherein R^la is as hereinbefore defined, with chloramine (NH₂C1), for example in the presence of an appropriate base (e.g. sodium hydride, sodium hydroxide or triethylamine) and a suitable solvent (such as diethyl ether, dioxane, dimethylformamide or dichloromethane), followed by reaction of the intermediate oxylamine (R^laONH₂) with a compound of formula XXIX,

R^lhV XXIX

1 II. wherein L and R are as hereinbefore defined, for example in the presence of a suitable base (e.g. sodium hydride, sodium bicarbonate, sodium hydroxide or triethylamine) and an appropriate organic solvent (e.g. dioxane, dichloromethane, dimethylformamide and/or acetone).

Compounds of formula XVII may be prepared by reductive amination of a compound of formula XXIII as hereinbefore defined, or an appropriate protected derivative thereof, in the presence of an amine of formula XXVI as hereinbefore defined, for example under standard conditions, such as those described hereinbefore for preparation of compounds of fonnula I.

Compounds of formula XIX may be prepared by conversion of the -OH group in a compound of formula XXX,

wherein Y_ls Y₂, Y_3s Y₄, Z_l3 Z₂, R³ and R^y are as hereinbefore defined, or an appropriate protected derivative thereof, to an appropriate leaving group, L¹ (e.g., in the case where L¹ is bromo, conversion may be carried out by reaction with CBr₄, for example at or around room temperature in the presence of a base (e.g. triphenylphosphine) and a suitable organic solvent (e.g. DMF)).

Compounds of formula XXI may be prepared from compounds of formula

XXXI,

wherein W¹ represents -CHO, -CH₂OH or -CH₂NH₂ and L², Y Y₂, Y₃ and Y₄ are as hereinbefore defined by way of standard techniques, for example by way of known techniques for the conversion of a -CHO, a -CH₂OH or a -CH₂NH₂ group into a

group (and in the case of -CHO and -CH₂OH groups analogously to methods described hereinbefore).

Compounds of formula XXII and protected derivatives thereof may be prepared by reaction of a corresponding compound of formula XXXII,

Z r ZH^RV XXXII

R³ wherein R^y, R³, Zi and Z₂ are as hereinbefore defined, or an appropriate protected derivative thereof, with a reagent system that will enable the introduction of -B(OH)₂ into the appropriate ring system. Suitable reagent systems include trialkylborates (e.g. tri-t_?o-propylborate). Such reactions may be carried out, for example, at low temperature (e.g. between -100°C and 0°C, e.g. between -80°C (such as -78°C) and -10°C (such as -20°C)) in the presence of a suitable base (e.g. n-butyl lithium) and an appropriate organic solvent (e.g. THF), followed by acid hydrolysis (e.g. in the presence of dilute HCl).

Compounds of formula XXV may be prepared from corresponding compounds of formula XXII as hereinbefore defined, for example using analogous methods to those described hereinbefore for conversion of the various R^y groups to the relevant R² groups (see, for example, processes for the preparation of compounds of formula I) .

Compounds of formulae XXIII and XXX may be prepared by reaction of a compound of formula XXXI as hereinbefore defined (in which former case, W¹ represents -CHO and in which latter case, W¹ represents -CH₂OH), with a compound of formula XXII as hereinbefore defined, or an appropriate protected derivative thereof, for example under similar conditions to those decribed hereinbefore for preparation of compounds of formulae II, VII, IX and XVI (third process).

Compounds of formula XXXII are available using known techniques. For example:

(a) Compounds of formula XXXII in which R^y represents -S(0)₂NH₂, -C(0)NH₂ or -CHO, and protected derivatives thereof, may be prepared by reaction of a compound of formula XXXIII, XXXIII

wherein R^ya represents -S(0)₂NH₂, -C(0)NH₂ or -CHO and Zi and Z₂ are as hereinbefore defined, or a protected derivative thereof, with a compound of formula XXXIV,

R³L³ XXXIV

wherein L represents a suitable leaving group (such as toluenesulphonate, benzenesulphonate, methanesulphonate or halo, such as bromo or iodo) and R is as hereinbefore defined, for example at below room temperature (e.g. between around -35°C and around -85°C), in the presence of a suitable base (e.g. n-butyl lithium) and an appropriate solvent (e.g. THF).

(b) Compounds of formula XXXII in which R^y is -S(0)₂NH₂ and N- protected derivatives thereof, may be prepared by reaction of an appropriate compound of formula XXXV,

wherein R , ∑ι and Z₂ are as hereinbefore defined with an appropriate reagent for introduction of a -S(0)₂NH₂ group into the appropriate ring system (for example chlorosulphonic acid., or thionyl chloride in the presence of a suitable strong base (e.g. butyl lithium)), followed by reaction of the resultant intermediate with ammonia, or a protected derivative thereof (e.g. tert-butylamine), under conditions that are well known to those skilled in the art.

(c) Certain protected derivatives (e.g. alkyl, such as C_1-6 alkyl, for example tert-butyl, protected derivatives) of compounds of formula XXXII in which R^y represents -C(0)NH₂ may be prepared by reaction of a compound of formula XXXV as hereinbefore defined, with a compound of formula XXXVI,

R^zN=C=0 XXXVI

wherein R^z represents an appropriate protecting group, such as an alkyl group, including C_1-6 alkyl, e.g. tert-butyl for example at around 0°C, in the presence of a suitable base (e.g. n-butyl lithium) and an appropriate solvent (e.g. THF).

(d) Certain protected derivatives (e.g. alkyl, such as C_1-6 alkyl, for example tert-butyl, protected derivatives) of compounds of formula XXXII in which R^y represents -C(0)NH₂ may also be prepared by reaction of a compound of formula XXXVII,

wherein R³, Zi and Z₂ are as hereinbefore defined with a protected (e.g. an (e.g. C_1-6) alkyl, such as tert-butyl-protected) derivative of ammonia (e.g. tert-butylamine) under standard coupling conditions

(see, for example, those described hereinbefore for preparation of compounds of formula I (process step (iii))). Compounds of formula XXXVII .are known in the art or may be prepared by way of standard techniques, for example oxidation of a corresponding compound of formula XXXII in which R^y is -CHO e.g. under those conditions described hereinbefore for preparation of compounds of formula V.

(e) Compounds of formula XXXII in which R^y is -CHO, Zj represents -CH=CH- and Z₂ represents -CH-, and protected derivatives thereof, may be prepared by reaction of a compound of formula XXXV in which Zj represents -CH=CH- and Z₂ represents -CH- with an appropriate reagent system for the introduction of an aldehyde group into the benzene ring (e.g. TiCl₄/CHCl₃, SnCl₄/CH₂Cl₂ or 1,3,5,7- azaadamantane/TFA) under standard reaction conditions, followed by (if appropriate) protection of the resultant benzaldehyde under standard conditions.

(f) Compounds of formula XXXII in which R^y is -NH₂, Zi represents -CH=CH~ and Z₂ represents -CH-, and N-protected derivatives thereof, may be prepared by nitration of a compound of formula XXXV in which Z_\ represents -CH=CH- and Z₂ represents -CH-, followed by reduction of the resultant nitrobenzene and (if appropriate) protection of the resultant aminobenzene, all of which steps may be carried out under standard conditions.

Compounds of formulae III, IV, VI, VIII, X, XI, XII, XIII, Xllla, XVIII,

XX, XXIV, XXVI, XXVII, XXVIII, XXIX, XXXI, XXXIII, XXXIV, XXXV and XXXVI are either commercially available, are known in the literature, or may be obtained either by analogy with the processes described herein, or by conventional synthetic procedures, in accordance with standard techniques, from readily available starting materials using appropriate reagents and reaction conditions.

Compounds of the invention may be isolated from their reaction mixtures using conventional techniques.

It will be appreciated by those skilled in the art that, in the processes described above and hereinafter, the functional groups of intermediate compounds may need to be protected by protecting groups.

Functional groups that it is desirable to protect include sulphonamido, amido, amino and aldehyde. Suitable protecting groups for sulphonamido, amido and amino include tert-butyloxycarbonyl, benzyloxycarbonyl, 2- trimethylsilylethoxycarbonyl (Teoc) or tert-butyl. Suitable protecting groups for aldehyde include alcohols, such as methanol or ethanol, and diols, such as 1,3-propanediol or, preferably, 1,2-ethanediol (so forming a cyclic acetal).

The protection and deprotection of functional groups may take place before or after a reaction in the above-mentioned schemes.

Protecting groups may be removed in accordance with techniques that are well known to those skilled in the art and as described hereinafter. For example, protected compounds/intermediates described herein may be converted chemically to unprotected compounds using standard deprotection techniques (e.g. using trifluoroacetic acid, sulfuric acid, toluenesulfonic acid or boron trichloride).

Persons skilled in the art will appreciate that, in order to obtain compounds of the invention in an alternative, and, on some occasions, more convenient, manner, the individual process steps mentioned hereinbefore may be performed in a different order, and/or the individual reactions may be performed at a different stage in the overall route (i.e. substituents may be added to and/or chemical transformations performed upon, different intermediates to those mentioned hereinbefore in conjunction with a particular reaction). This may negate, or render necessary, the need for protecting groups.

The type of chemistry involved will dictate the need, and type, of protecting groups as well as the sequence for accomplishing the synthesis.

The use of protecting groups is fully described in "Protective Groups in Organic Chemistry", edited by J W F McOmie, Plenum Press (1973), and "Protective Groups in Organic Synthesis", 3^rd edition, T.W. Greene & P.G.M. Wutz, Wiley-Interscience ( 1999) .

Medical and Pharmaceutical Uses

Compounds of the invention are useful because they possess pharmacological activity. The compounds of the invention are therefore indicated as pharmaceuticals.

According to a further aspect of the invention there is thus provided the compounds of the invention for use as pharmaceuticals.

In particular, compounds of the invention are agonists of Angll, more particularly, are agonists of the AT2 receptor, and, especially, are selective agonists of that sub-receptor, for example as may be demonstrated in the tests described below. The compounds of the invention are thus expected to be useful in those conditions in which endogenous production of Angll is deficient and/or where an increase in the effect of Angll is desired or required.

The compounds of the invention are further expected to be useful in those conditions where AT2 receptors are expressed and their stimulation is desired or required.

The compounds of the invention are further indicated in the treatment of conditions characterised by vasoconstriction, increased cell growth and/or differentiation, increased cardiac contractility, increased cardiovascular hypertrophy, and/or increased fluid and electrolyte retention.

The compounds of the invention are further indicated in the treatment of stress-related disorders, and/or in the improvement of microcirculation and or mucosa-protective mechanisms.

Thus, compounds of the invention are expected to be useful in the treatment of disorders, which may be characterised as indicated above, and which are of, for example, the gastrointestinal tract, the cardiovascular system, the respiratory tract, the kidneys, the eyes, the female reproductive (ovulation) system and the central nervous system (CNS).

Disorders of the gastrointestinal tract that may be mentioned include oesophagitis, Barrett's oesophagus, gastric ulcers, duodenal ulcers, dyspepsia (including non-ulcer dyspepsia), gastro-oesophageal reflux, irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), pancreatitis, hepatic disorders (such as hepatitis), gall bladder disease, multiple organ failure (MOF) and sepsis. Other gastrointestinal disorders that may be mentioned include xerostomia, gastritis, gastroparesis, hyperacidity, disorders of the bilary tract, coelicia, Crohn's disease, ulcerative colitis, diarrhoea, constipation, colic, dysphagia, vomiting, nausea, indigestion and Sjδgren's syndrome.

Disorders of the respiratory tract that may be mentioned include inflammatory disorders, such as asthma, obstructive lung diseases (such as chronic obstructive lung disease), pneumonitis, pulmonary hypertension and adult respiratory distress syndrome.

Disorders of the kidneys that may be mentioned include renal failure, nephritis and renal hypertension.

Disorders of the eyes that may be mentioned include diabetic retinopathy, premature retinopathy and retinal microvascularisation.

Disorders of the female reproductive system that may be mentioned include ovulatory dysfunction.

Cardiovascular disorders that may be mentioned include hypertension, cardiac hypertrophy, cardiac failure, artherosclerosis, arterial thrombosis, venous thrombosis, endothelial dysfunction, endothelial lesions, post- balloon dilatation stenosis, angiogenesis, diabetic complications, microvascular dysfunction, angina, cardiac arrhythmias, claudicatio intermittens, preeclampsia, myocardial infarction, reinfarction, ischaemic lesions, erectile dysfunction and neointima proliferation.

Disorders of the CNS that may be mentioned include cognitive dysfunctions, dysfunctions of food intake (hunger/satiety) and thirst, stroke, cerebral bleeding, cerebral embolus and cerebral infarction. Compounds of the invention may also be useful in the modulation of growth metabolism and proliferation, for example in the treatment of hypertrophic disorders, prostate hyperplasia, autoimmune disorders, psoriasis, obesity, neuronal regeneration, the healing of ulcers, inhibition of adipose tissue hypeφlasia, stem cell differentiation and proliferation, cancer (e.g. in the gastrointestinal tract, lung cancer, etc), apoptosis, tumours (generally) and hypertrophy, diabetes, neuronal lesions and organ rejection.

The compounds of the invention are indicated both in the therapeutic and/or prophylactic treatment of the above conditions.

According to a further aspect of the present invention, there is provided a method of treatment of a condition in which endogenous production of Angll is deficient, and/or a condition where an increase in the effect of Angll is desired or required, and/or a condition where AT2 receptors are expressed and their stimulation is desired or required, which method comprises administration of a therapeutically effective amount of a compound of the invention to a person suffering from, or susceptible to, such a condition.

The compounds of the invention will normally be administered orally, intravenously, subcutaneously, buccally, rectally, dermally, nasally, tracheally, bronchially, by any other parenteral route or via inhalation, in a pharmaceutically acceptable dosage form.

When the condition to be treated is multiple organ failure, preferred routes of administration are parenteral (e.g. by injection). Otherwise, the preferred route of administration for compounds of the invention is oral. The compounds of the invention may be administered alone, but are preferably administered by way of known pharmaceutical formulations, including tablets, capsules or elixirs for oral administration, suppositories for rectal administration, sterile solutions or suspensions for parenteral or intramuscular administration, and the like.

Such formulations may be prepared in accordance with standard and/or accepted pharmaceutical practice.

According to a further aspect of the invention there is thus provided a pharmaceutical formulation including a compound of the invention, in admixture with a pharmaceutically acceptable adjuvant, diluent or carrier.

Compounds of the invention may also be administered in combination with other AT2 agonists that are known in the art, as well as in combination with ATI receptor antagonists that are known in the art, such as losartan, or in combination with an inhibitor of angiotensin converting enzyme (ACE).

According to a further aspect of the invention, there is provided a combination product comprising:

(A) a compound of the invention; and

(B) an ATI receptor antagonist, or an ACE inhibitor, wherein each of components (A) and (B) is formulated in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier.

Such combination products provide for the administration of compound of the invention in conjunction with an ATI receptor antagonist, or an ACE inhibitor, and may thus be presented either as separate formulations, wherein at least one of those formulations comprises compound of the invention, and at least one comprises ATI receptor antagonist, or ACE inhibitor, or may be presented (i.e. formulated) as a combined preparation (i.e. presented as a single formulation including compound of the invention and ATI receptor antagonist or ACE inhibitor).

Thus, there is further provided:

(1) a pharmaceutical formulation including a compound of the invention and an ATI receptor antagonist, or an ACE inhibitor, in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier; and

(2) a kit of parts comprising components:

(a) a pharmaceutical formulation including a compound of the invention, in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier; and (b) a pharmaceutical formulation including an ATI receptor antagonist, or an ACE inhibitor, in admixture with a pharmaceutically- acceptable adjuvant, diluent or carrier, which components (a) and (b) are each provided in a form that is suitable for administration in conjunction with the other.

Depending upon the disorder and patient to be treated and the route of administration, the compounds of the invention may be administered at varying doses.

Although doses will vary from patient to patient, suitable daily doses are in the range of about 1 to 1000 mg per patient, administered in single or multiple doses. More preferred daily doses are in the range 2.5 to 250 mg per patient. Individual doses of compounds of the invention may be in the range 1 to 100 mg.

In any event, the physician, or the skilled person, will be able to determine the actual dosage which will be most suitable for an individual patient, which is likely to vary with the condition that is to be treated, as well as the age, weight, sex and response of the particular patient to be treated. The above-mentioned dosages are exemplary of the average case; there can, of course, be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention.

Compounds of the invention have the advantage that they bind selectively to, and exhibit agonist activity at, the AT2 receptor. By compounds which "bind selectively" to the AT2 receptor, we include that the affinity ratio for the relevant compound (AT2:AT1) is at least 5:1, preferably at least 10:1 and more preferably at least 20: 1.

The compounds of the invention may also have the advantage that they may be more efficacious than, be less toxic than, be longer acting than, be more potent than, produce fewer side effects than, be more easily absorbed than, and/or have a better pharmacokinetic profile (e.g. higher oral bioavailability and/or lower clearance) than, and/or have other useful pharmacological, physical, or chemical properties over, compounds known in the prior art.

Biological Tests

The following test procedures may be employed. Test A

Receptor Binding Assay using Rat Liver Membrane AT^ Receptor Rat liver membranes were prepared according to the method of Dudley et al (Mol. Pharmacol. (1990) 38, 370). Binding of [¹²⁵I]Ang II to membranes was conducted in a final volume of 0.5 mL containing 50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 10 mM MgCl₂, 1 mM EDTA, 0.025% bacitracin, 0.2% BSA (bovine serum albumin), liver homogenate corresponding to 5 mg of the original tissue weight, [¹²⁵I]Ang II (70 000 cpm, 0.03 nM) and variable concentrations of test substance. Samples were incubated at 25°C for 1 h, and binding was terminated by filtration through Whatman GF/B glass-fiber filter sheets using a Brandel cell harvester. The filters were washed with 4 x 2 mL of Tris-HCl (pH 7.4) and transferred to tubes. The radioactivity was measured in a gamma counter. The characteristics of the Ang II binding ATi receptor were determined by using six different concentrations (0.03-5

19^ nmol/L) of the labeled [ I] Angll. Non-specific binding was determined in the presence of 1 μM Ang II. The specific binding was determined by subtracting the non-specific binding from the total bound [ IJAngIL The dissociation constant (ΛT_ = 1-7 ± 0.1 nM, [L] = 0.057 nM) was determined by Scatchard analysis of data obtained with Ang II by using GraFit (Erithacus Software, UK). The binding data were best fitted with a one-site fit. All experiments were performed at least in triplicate.

Test B

Receptor Binding Assay using Porcine Mvometrial Membrane AT Receptor

Myometrial membranes were prepared from porcine uteri according to the method by Nielsen et al (Clin. Exp. Pharm. Phys. (1997) 24, 309). Any possible interference that may be exhibited by binding of compound to ATi receptors was blocked by addition of 1 μM of a selective ATI inhibitor. Binding of [ I]Ang II to membranes was conducted in a final volume of 0.5 mL containing 50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 10 mM MgCl₂, 1 mM EDTA, 0.025% bacitracin, 0.2% BSA, homogenate

1 9*. corresponding to 10 mg of the original tissue weight, [ I] Ang II (70 000 cpm, 0.03 nM) and variable concentrations of test substance. Samples were incubated at 25°C for 1 h, and binding was terminated by filtration through Whatman GF/B glass-fiber filter sheets using a Brandel cell harvester. The filters were washed with 3 x 3 mL of Tris-HCl (pH 7.4) and transferred to tubes. The radioactivity was measured using a gamma counter. The characteristics of the Ang II binding AT₂ receptor was determined by using six different concentrations (0.03-5 nmol/L) of the labeled [ I]Ang II. Non-specific binding was determined in the presence of 1 μM Ang II. The specific binding was determined by subtracting the non-specific binding from the total bound [ I]Ang II. The dissociation constant (K_d = 0.7 ± 0.1 nM, [L] = 0.057 nM) was determined by Scatchard analysis of data obtained with Ang II by using GraFit (Erithacus Software, UK). The binding data were best fitted with a one-site fit. All experiments were performed at least in triplicate .

Test C Duodenal Mucosal Alkaline Secretion Assay

Compounds were exposed to the duodenal mucosa in barbiturate- anaesthetised rats prepared for in situ titration of duodenal mucosal alkaline secretion, according to the methodology described by Flemstrδm et al in Am. J. Physiol. (1982) 243, G348.

The invention is illustrated by way of the following examples. Preparation A 3-(4-Formylphenyl)-5-Mo-butyl-N-tert-butylthiophene-2-sulfonamide

(a) N-tert-Butylthiophene-2-sulfonamide Thiophene-2-sulfonyl chloride (15 g, 0.082 mol) was dissolved in CHC1₃ (200 mL) under N₂ atmosphere and then cooled to 0°C. te/'t-Butylamine (25.9 mL, 0.246 mol) dissolved in CHC1₃ (50 mL) was then added dropwise to the reaction mixture. The reaction mixture was stirred for 1 h at room temperature and then at reflux for 10 min. Toluene (700 mL) was added and the organic phase was washed with water (3 x 50 mL), dried, and concentrated in vacuo. The sub-title product was used without further purification in the next step.

1H NMR (CDC1₃) δ 7.60(1H, dd, J=1.3, 3.8 Hz), 7.53(1H, dd, J=1.3, 5.0 Hz), 7.02(1H, dd, J=5.0, 3.8 Hz), 5.13(1H, m), 1.24 (9H, m) ¹³C NMR (CDCl₃) δ 145.0, 131.7, 131.2, 127.0, 55.1, 29.9

(b) 5-*so-Butyl-N-tert-butylthiophene-2-sulfonamide

N-tert-Butylthiophene-2-sulfonamide (10 g, 0.046 mol, see step (a) above) was dissolved in THF (85 mL) under Ν₂ and then cooled to -78°C. n-BuLi (1.6 M, 76.9 mL, 0.12 mol) was added via a syringe. The reaction mixture was stirred at -78°C for 30 min. and then at -40°C for 2 h. Iodo-2- methylpropane (10.5 mL, 0.09 mol) was added dropwise to the reaction mixture. The reaction mixture was stirred overnight at room temperature. The reaction was quenched with NH₄C1 (aq.) and extracted with EtOAc. The combined organic phase was washed with brine and dried and concentrated in vacuo. The crude product was purified on column chromatography (hexanes:EtOAc (10:1)) to give the sub-title compound in 55% yield (7.0 g, 0.025 mol). 1H NMR (CDCI₃) δ 7.43(1H, d, J= 3.6 Hz), 6.67(1H, d, J=3.8 Hz), 4.83(1H, m), 2.67(2H, d, J=7 Hz), 1.88 (IH, m), 1.26(9H, m), 0.93(6H, J=6.6 Hz). ¹³C NMR (CDCI₃) δ 145.0, 131.7, 131.2, 127.0, 55.1, 29.9

(c) 5-t^'_o-Butyl-2-(N-tert-butylaminosulfonyl thiophene-3-boronic acid

5-wo-Butyl-N-tert-butylthiophene-2-sulfonamide (10.6 g, 0.039 mol, see step (b) above) was dissolved in THF (165 mL) under N₂ and then cooled to -78°C. n-BuLi (1.6 M, 60.19 mL, 0.096 mol) was added via a syringe. The reaction mixture was stirred at -20°C for 4 h. The tri-wo-propylborate (13.3 mL, 0.058 mol) was then added via a syringe and the reaction mixture was stirred overnight at room temperature. The reaction was quenched with 2 M HCl (20 mL). The organic phase was separated and the water phase was extracted with EtOAc (3 x 100 mL). The combined organic phase was washed with brine, dried and concentrated in vacuo. The product may be used without further purification. MS(ESI⁺) m z: 236.8

(d 3-(4-Formylphenyl)-5-t_?<. -butyl-N-tert-butylthiophene-2-sulfonamide Palladium acetate (69.6 mg, 0.31 mmol) and triphenylphosphine (0.33 g, 1.24 mmol) in THF (5 mL) were stirred for 30 min under N₂(g). The solvent was removed in vacuo and the residue was dissolved in DME (5 mL). The catalyst was then transferred into a nitrogen- flushed mixture of 5- .5_)-butyl-2-(N-tert-butylaminosulfonyl)-thiophene-3-boronic acid (1.11 g, 3.13 mmol, based on 90% purity, see step (c) above), 4-bromobenzaldehyde (1.45 g, 7.84 mmol) and potassium carbonate (1.73 g, 12.5 mmol) in a solvent mixture of DME (10 mL), ethanol (3 mL), and water (2 mL). After stirring for 20 h at reflux under a N₂ atmosphere, the reaction mixture was diluted with 1M NaOH solution (20 mL) followed by ethyl acetate (70 mL). The organic layer was washed with water, and brine, dried over anhydrous MgS0₄, concentrated in vacuo, and the residue subjected to flash chromatography (20% ethyl acetate in petroleum ether, 230-400 mesh) to afford the title compound as colourless solid (0.76 g, 64%). IR (neat, cm^"1) v 3284, 2963, 1702, 1606

1H NMR (CDC1₃) δ 0.97 (d, J = 6.6 Hz, 6H), 1.01 (s, 9H), 1.94 (m, IH), 2.68 (d, J= 6.6 Hz, 2H), 4.24 (s, IH), 6.78 (s, IH), 7.78 (d, J= 8.3 Hz, 2H), 7.95 (d, J= 6.6 Hz, 2H), 10.04 (s, IH)

¹³C NMR (CDCI₃) δ 22.1, 29.6, 30.5, 39.1, 54.7, 128.7, 129.6, 129.8, 135.7, 137.5, 141.0, 141.8, 149.0, 191.8 MS (ESI⁺) m/z: 379.9 (M⁺ +1) Anal. Calcd for C₁₉H₂₅N0₃S₂: C, 60.13; H, 6.64, N, 3.69; O, 12.65; S, 16.9; Found C, 59.9; H, 6.6; N, 3.6; O, 12.4; S, 16.8

Examples 1 to 12 General Procedure

Step I: The appropriate amine (1.1 eqv., 0.09 mmol, see below) was added to a solution of 3-(4-formylphenyl)-5- ,sO-butyl-N-tert-butylthiophene-2- sulfonamide (30 mg, 0.08 mmol, see Preparation A above) in methanol (1.5 mL) in a sample vial (5 mL size). After being stirred for 2 h, sodium borohydride (6.1 mg, 0.16 mmol) was added and the stirring continued for 2 h. The mixture was acidified with dilute HCl (5 N, 0.1 mL), stirred for 10 min, neutralised with saturated NaHC0₃ solution (~0.5 mL) and diluted with ethyl acetate (10 mL). The contents were poured into diatomaceous earth (liquid-liquid extraction cartridge) in a polypropylene column (packed for 1.5 cm, 24 mL size) and eluted with ethyl acetate (30 mL). Concentration under vacuum afforded the crude product.

Step 2: The product from step 1 was dissolved in dry DCM (1.5 mL) in a sample vial (5 mL size). Triethylamine (0.033 mL, 0.24 mmol), DMAP (1 mg, 0.008 mmol) and the appropriate acid chloride or alkyl chloroformate (2 eqv., 0.16 mmol, see below) were then added sequentially. The sample vial was tightly closed. The mixture was stirred overnight, quenched with aqueous saturated NaHC0₃ solution (0.5 mL), stirred for 30 min, and filtered through diatomaceous earth (packed for 1.5 cm in the column of 24 mL capacity) on elution with DCM (30 mL). Concentration in vacuo afforded the crude product.

Step 3: The mixture of the product from step 2 and anisole (~2 drops) in trifluoroacetic acid (1.5 mL) in a sample vial (5 mL size) was stirred at 30°C overnight. After the removal of the solvent in vacuo, the residue was dissolved in acetonitrile (2 mL) and evaporated (2 x).

Step 4: To a mixture of the product from step 3 in dry DCM (1.5 mL), pyrrollidinopyridine (17.8 mg, 0.12 mmol) and triethylamine (0.5 mL, 0.36 mmol), n-butyl chloroformate (0.04 mL, 0.3 mmol) were sequentially added. The solution was stirred for 12 h, concentrated in vacuo and the crude product purified by LCMS (30% acetonitrile to pure acetonitrile, reverse phase) to afford the title products indicated below:

Example 1

N-Butyloxycarbonyl-3-[4-(N-acetyl-N-benzylaminomethyl)phenyl]-5-/i'o- butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using benzylamine and acetyl chloride. The crude product from the final step was purified by LCMS (25% aqueous acetonitrile to pure acetonitrile, reverse phase) to afford a colourless solid (30 mg, 68%).

IR(neat, cm^"1) v 2955, 1747, 1629, 1451

1H ΝMR (CDC1₃) δ 0.86 (t, _/ = 7.3 Hz, 3H)₃0.98 (d, J = 7.4 Hz, 6H), 1.25

(m, 2H), 1.49 (m, 2H), 1.94 (m, IH), 2.2 (d, 3H, J = 3.63 Hz), 2.66- 2.75 (m, 2H), 3.98- 4.08 (m, 2H), 4.46- 4.60 (m, 4H), 6.75 (d, IH, J= 8.58 Hz), 7.14- 7.51 (m, 9H), 8.25 (brd, IH)

¹³C NMR (CDC1₃) δ 13.6, 18.7, 21.5, 22.2, 29.6, 30.4, 30.5, 39.3, 48.0, 50.5, 51.1, 66.7, 66.8, 126.3, 127.4, 127.7, 128.0, 128.3, 128.6, 129.0, 129.1, 129.4, 129.5, 130.7, 133.2, 133.4, 136.0, 136.9, 137.0, 137.6, 145.9, 146.1, 150.3, 151.3, 151.5, 171.4, 171.6 MS (ESI⁺) m/z: 557.3 (M⁺ +1)

Anal. Calcd for C₂₉H₃₆N₂0₅S₂: C, 62.56; H, 6.52; N, 5.03; Found C, 62.8; H, 6.7; N, 5.0

Example 2

N-Butyloxycarbonyl-3-[4-(N-benzylpentylamidomethyl)phenyl]-5-/ ^,o- butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using benzylamine and valeroyl chloride. The crude product from the final step was purified by LCMS (30% aqueous acetonitrile to pure acetonitrile, reverse phase) to afford a colourless solid (26 mg, 55%).

IR (neat, cm^'1) v 2959, 2871, 1748, 1626, 1453

1H NMR (CDCI₃) δ 0.83-1.03 (m, 12H), 1.17-1.44 (m, 6H), 1.68 (m, 2H), 1.95 (m, IH), 2.44 (dt, J= 1.25, 8.3 Hz, 2H), 2.66-2.74 (m, 2H), 4.05 (q, J=

5.3 Hz, 2H), 4.43-4.68 (m, 4H), 6.76 (d, J= 7.9 Hz, IH), 7.1-7.50 (m, 9H),

7.60-7.95 (brs, IH)

¹³C NMR (CDCI₃) δ 13.6, 13.9, 18.7, 22.2, 22.5, 27.5, 30.4, 30.5, 33.0,

39.3, 48.1, 49.7, 50.4, 66.8, 66.9, 126.4, 127.4, 127.7, 128.0, 128.3, 128.6, 128.9, 129.1, 129.5, 130.6, 133.0, 133.3, 136.3, 137.2, 137.3, 138.0, 146.1,

146.2, 150.2, 151.4, 151.6, 173.9, 174.1

MS (ESI⁺) m/z: 598.8 (M⁺ +1)

Anal. Calcd for C₃₂H₄₂N₂0₅S₂: C, 64.18; H, 7.07; N, 4.68; Found C, 63.8;

H, 7.0; N, 4.70 Example 3

N-Butyloxycarbonyl-3-[4-(N-j?-tolylbenzylamidomethyl phenyl]-5-.^'^o- butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using j_?-tolylamine and benzoyl chloride. The crude product from the final step was purified by LCMS (45% aqueous acetonitrile to pure acetonitrile, reverse phase) to afford a colourless solid (49 mg, 92%).

IR (neat, cm^"1) v 2960, 1748, 1628, 1511, 1447

1H NMR (CDC1₃) δ 0.85 (t, J= 7.6 Hz, 3H), 0.98 (d, J = 6.6 Hz, 6H), 1.24 (m, 2H), 1.48 (m, 2H), 1.93 (m, IH), 2.23 (s, 3H), 2.69 (d, J= 6.9 Hz, 2H),

3.99 (t, J= 6.6 Hz, 2H), 5.12 (s, 2H), 6.76 (s, IH), 6.82 (d, J= 8.6 Hz, 2H),

6.95 (d, J= 7.9 Hz, 2H), 7.1-7.24 (m, 3H), 7.31-7.44 (m, 6H)

¹³C NMR (CDC1₃) δ 13.6, 18.7, 20.9, 22.3, 30.4, 39.5, 53.8, 66.8, 127.4,

127.8, 128.2, 128.8, 129.0, 129.3, 129.7, 133.0, 135.7, 136.7, 138.2, 140.8, 146.1, 146.9, 151.4, 170.6

MS (ES ) m/z: 618.8 (M⁺ +1)

Anal. Calcd for C₃₄H₃₈N₂0₅S₂: C, 65.99; H, 6.19; N, 4.53 Found C, 65.8; H,

6.4; N, 4.3

Example 4

N-Butyloxycarbonyl-3-[4-(N-acetyl-N-p-tolylaminomethyl phenyl]-5-t O- butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using -tolylamine and acetyl chloride. The crude product from the final step was purified by LCMS (40% aqueous acetonitrile to pure acetonitrile, reverse phase) to afford a colourless solid (44 mg, 68%).

IR (neat, cm^"1) v 2960, 1747, 1636, 1513, 1465

1H NMR (CDCI₃) δ 0.86 (t, J= 7.3 Hz, 3H), 0.98 (d, J= 6.6 Hz, 6H), 1.24

(m, 2H), 1.48 (m, 2H), 1.82-12.1 (m, 4H), 2.33 (s, 3H), 2.69 (d, J= 7.26 Hz, 2H), 4.02 (t, J= 6.6 Hz, 2H), 4.83 (s, 2H), 6.76 (s, IH), 6.88 (d, J= 8.3 Hz, 2H), 7.13 (d, J= 8.3 Hz, 2H), 7.23 (d, J= 7.9 Hz, 2H), 7.39 (d, J= 8.3 Hz, 2H), 8.36 (s, IH)

¹³C NMR (CDC1₃) δ 13.6, 18.8, 21.1, 22.2, 22.5, 29.7, 30.5, 39.3, 52.6, 66.7, 127.8, 128.7, 128.9, 129.3, 130.2₅ 130.9, 133.1, 138.0, 140.0, 146.0, 150.3, 151.2, 171.1

MS (ESI⁺) m z: 556.8 (M⁺ +1)

Anal. Calcd for C₂₉H₃₆N₂0₅S₂.l/2 H₂0: C, 61.6; H, 6.6; N, 4.9 Found: C,

61.7; H, 6.5; N, 4.8

Example 5

N-Butyloxycarbonyl-3-{4-[N-(pyridin-3-ylmethyl)benzylamidomethyl]- phenyl}-5-/ ^ιQ-butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using 3-picolylamine and benzoyl chloride. The crude product from the final step was purified by LCMS (45% aqueous acetonitrile to pure acetonitrile, reverse phase) to afford a colourless solid (45 mg, 92%).

IR (neat, cm^"1) v 2959, 1740, 1635, 1457, 1410

1H NMR (CDCI₃) δ 0.89 (t, _7 = 7.3 Hz, 3H), 0.97 (d, J= 6.6 Hz, 6H), 1.30

(m, 2H), 1.55 (m, 2H), 1.92 (m, IH), 2.68 (d, J = 6.9 Hz, 2H), 4.08 (t, J = 6.6 Hz, 2H), 4.58- 4.82 (m, 4H), 6.6 (br s, IH), 6.9- 7.54 (m, 11H), 8.23-

8.43 (m, 2H)

¹³C NMR (CDCI₃) δ 13.7, 18.9, 22.2, 30.5, 30.7, 39.2, 48.1, 50.9, 53.5,

55.9, 65.9, 123.5, 123.9, 126.1, 126.5, 128.3, 128.8, 129.3, 129.7, 133.0,

134.1, 134.8, 135.9, 136.7, 139.9, 144.4, 146.5, 147.5, 147.3, 148.3, 148.4, 150.3, 152.0, 172.1

MS (ESI⁺) m/z: 620.1 (M⁺ +1)

Anal. Calcd for C₃₃H₃₇N₃O₅S₂: C, 63.95; H, 6.02; N, 6.78 Found C, 63.6; H,

6.2; N, 6.4 Example 6

N-Butyloxycarbonyl-3 - [4-(N-acetyl-N-ρyridin-3 -ylme thylaminome thy!)- phenyl]-5-/^'_?o-butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using 3-ρicolylamine and acetyl chloride. The crude product from the final step was purified by LCMS (45% aqueous acetonitrile to 90% acetonitrile, reverse phase) to afford a colourless syrup (38 mg, 86%).

IR (neat, cm^"1) v 2960, 1742, 1650, 1429

1H ΝMR (CDC1₃) δ 0.89 (t, J= 7.6 Hz, 3H), 0.97 (d, J = 6.6 Hz, 6H), 1.26 (m, 2H), 1.53 (m, 2H), 1.93 (m, IH), 2.27 (m, 3H), 2.68 (d, J= 6.9 Hz, 2H),

4.07 (m, 2H), 4.49 (s, 2H), 4.65 (t, J- 6.9 Hz, 2H), 6.68 (m, IH), 6.93- 7.36

(m, 6H), 7.61- 8.45 (m, 2H)

¹³C ΝMR (CDCI₃) δ 13.6, 18.8, 22.2, 30.4, 30.6, 39.2, 48.2, 50.8, 52.2,

54.9, 65.8, 123.6, 127.2, 128.0, 128.5, 129.3, 129.7, 132.0, 132.5, 133.1, 133.6, 133.9, 134.7, 136.1, 136.4, 138.7, 139.9, 144.3, 145.0, 146.2, 147.4,

147.9, 148.2, 150.3, 151.6, 152.0, 170.6, 170.8

MS (ESI⁺) m/z: 558.2 (M⁺ +1)

Anal. Calcd for CasfeΝaOsSa- V2 H₂0: C, 59.30; H, 6.4; Ν, 7.4 Found C,

59.5; H, 6.1; Ν, 7.4

Example 7

Ν-Butyloxycarbonyl-3-[4-(Ν-methylpentylamidomethyl)phenyl1-5-iso- butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using methylamine and valeroyl chloride. The crude product from the final step was purified by LCMS (30% aqueous acetonitrile to 85% acetonitrile, reverse phase) to afford a colourless syrup (26 mg, 63%).

IR (neat, cm^"1) v 2959, 1747, 1628, 1466

1H NMR (CDCI₃) δ 0.82-1.02 (m, 12H), 1.17- 1.56 (m, 6H), 1.66 (m, 2H), 1.93 (m, IH), 2.38 (m, 2H), 2.65- 2.74 (d, J = 6.9 Hz, 2H), 2.95 (m, 3H), 4.02 (t, J = 6.6 Hz, 2H), 4.57 (s, 2H), 6.75 (m, IH), 7.22 (m, 2H), 7.44 (m, 2H)

¹³C NMR (CDC1₃) δ 13.6, 13.9, 18.7, 22.2, 22.6, 27.2, 27.5, 30.4, 30.5, 32.9, 33.2, 33.9, 35.2, 39.3, 50.6, 53.1, 66.7, 66.8, 126.2, 127.7, 129.1, 129.4, 130.7, 133.0, 133.3, 137.3, 138.0, 146.1, 150.3, 151.3, 151.5, 173.6, 173.7

MS (ESI⁺) m z: 522.9 (M⁺ +l)

Anal. Calcd for C₂₆H₃₈N₂0₅S₂: C, 59.74; H, 7.33; N, 5.36 Found C, 59.8; H, 7.5; N, 5.5

Example 8

N-Butyloxycarbonyl-3-[4-(N-acetyl-N-methylaminomethyl)phenyl]-5-.^'.s^,o- butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using methylamine and acetyl chloride. The crude product from the final step was purified by LCMS (35% aqueous acetonitrile to 85% acetonitrile, reverse phase) to afford a colourless syrup (19 mg, 50%).

IR (neat, cm^"1) v 2960, 1746, 1628, 1466

1H ΝMR (CDCI₃) δ 0.81 (t, J= 7.3 Hz, 3H), 0.92 (d, J= 6.6 Hz, 6H), 1.18 (m, 2H), 1.44 (m, 2H), 1.87 (m, IH), 2.09 (m, 3H), 2.65 (m, 2H), 2.90 (m,

3H), 3.97 (m, 2H), 4.52 (m, 2H), 6.69 (m, IH), 7.17 (m, 2H), 7.39 (m, 2H)

¹³C ΝMR (CDCI₃) δ 13.6, 18.8, 21.4, 21.7, 22.2, 30.4, 30.5, 33.8, 35.8,

39.3, 50.5, 54.0, 66.7, 66.8, 126.2, 127.8, 129.2, 129.4, 129.5, 130.9, 133.2,

133.5, 137.0, 137.7, 146.0, 150.5, 151.2, 151.5, 171.2 MS (ESI⁺) m/z: 480.8 (M⁺ +1)

Anal. Calcd for C₂₃H₃₂Ν₃θ₅S₂: C, 57.47; H, 6.71; N, 5.83 Found: C, 57.0;

H, 6.7; N, 5.8 Example 9

N-Butyloχycarbonyl-3-r4-(N-ethyl-thiophenecarbonylaminomethyl)- ρhenyl]-5-t^'so-butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using ethylamine and thiophenecarboxyl chloride. The crude product from the final step was purified by LCMS (45% aqueous acetonitrile to 90% acetonitrile, reverse phase) to afford a colourless syrup

(20 mg, 45%).

IR (neat, cm^"1) v 2960, 1748, 1604, 1436 1H NMR (CDC1₃) δ 0.87 (t, J= 7.3 Hz, 3H), 0.99 (d, J= 6.6 Hz, 6H,), 1.24

(m, 5H), 1.50 (m, 2H), 1.95 (m, IH), 2.71 (d, J= 7.3 Hz, 2H), 3.58 (q, J =

6.9 Hz, 2H), 4.03 (t, J= 6.6 Hz, 2H), 6.78 (s, IH), 7.02 (t, J= 4.1 Hz, IH),

7.29- 7.50 (m, 6H)

¹³C NMR (CDCI₃) δ 13.6, 18.7, 22.2, 30.5, 39.3, 66.9, 126.9, 127.1, 128.6, 129.1, 129.3, 130.6, 133.2, 137.8, 146.1, 150.0, 151.6, 164.6

MS (ESI⁺) m/z: 563.2 (M⁺ +1)

Anal. Calcd for C₂₇H₃₄N₂0₅S₃: C, 57.62; H, 6.09; N, 4.98; Found: C, 58.1;

H, 6.5; N, 4.6

Example 10

N-Butyloxycarbonyl-3-[4- N-acetyl-N-ethylaminomethyl)phenyl]-5-/.s^,o- butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using ethylamine and acetyl chloride. The crude product in the final step was purified by LCMS (25% aqueous acetonitrile to 90% acetonitrile, reverse phase) to afford a colourless solid (18 mg, 46%).

IR (neat, cm^"1) v 2960, 1747, 1627, 1463

1H ΝMR (CDCI₃) δ 0.87 (t, J= 7.3 Hz, 3H), 0.98 (d, 6.6 Hz, 6H), 1.08- 1.34

(m, 5H), 1.50 (m, 2H), 1.94 (m, IH), 2.14 (m, 3H), 2.69 (m, 2H), 3.38 (m, 2H), 4.04 (m, 2H), 4.58 (m, 2H), 6.75 (m, IH), 7.14- 7.60 (m, 4H) ¹³C NMR (CDCI₃) δ 12.7, 13.6, 18.8, 21.2, 21.8, 22.2, 30.5, 39.3, 40.9, 42.9, 47.7, 51.3, 66.7, 66.8, 126.2, 127.7, 127.9, 129.2, 129.4, 130.9, 133.1, 133.4, 137.6, 138.4, 146.1, 150.4, 151.3, 151.5, 170.7 MS (ESI⁺) m/z: 495.1 (M⁺ +1) Anal. Calcd for C₂₄H₃₄N₂O₅S₂: C, 58.27; H, 6.93; N, 5.66; Found: C, 58.7; H, 7.1; N, 5.8

Example 11 N-Butyloxycarbonyl-3- 4- N-methanesulfonyl-N-methylaminomethyl)- phenyl]-5-t^'-o-butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using methylamine and methanesulfonyl chloride. The crude product from the final step was purified by LCMS (35% aqueous acetonitrile to 70% acetonitrile, reverse phase) to afford a colourless syrup (40 mg, 59%).

IR (neat, cm^"1) v 3208, 2960, 1749, 1458

1H ΝMR (CDCI₃) δ 0.87 (t, J= 7.3 Hz, 3H), 0.99 (d, J= 6.6 Hz, 6H), 1.25 (m, 2H), 1.50 (m, 2H), 1.94 (m, IH), 2.71 (d, J= 7.3 Hz, 2H), 2.80 (s, 3H), 2.88 (s, 3H), 4.04 (t, J = 6.6 Hz, 2H), 4.34 (s, 2H), 6.77 (s, IH), 7.35 (s, IH), 7.40 (d, J- 8.5 Hz, 2H), 7.47 (d, J= 8.5 Hz, 2H)

¹³C ΝMR (CDCI₃) δ 13.6, 18.7, 22.2, 30.4, 30.5, 34.5, 36.0, 39.3, 53.5, 66.9, 128.2, 129.3, 129.4, 130.6, 133.8, 136.3, 146.0, 150.0, 151.7 MS (ESI⁺) m/z: 517.2 (M⁺ +1)

Example 12

N-Butyloxycarbonyl-3-{4-rN-(ethyloxycarbonyl -N-methylaminomethyl]- phenyl}-5-t O-butylthiophene-2-sulfonamide

The title compound was synthesised as stated in the above general procedure using methylamine and ethyl chloroformate. The crude product from the final step was purified by LCMS (45% to 75% aqueous acetonitrile, reverse phase) to afford a colourless oil (40 mg, 74%).

1H NMR (270 MHz, CDC1₃): δ 0.87 (t, 3H, J = 7.26 Hz), 0.98 (d, 6H, J = 6.60 Hz), 1.17- 1.30 (m, 5H), 1.49 (m, 2H), 1.94 (m, IH), 2.70 (d, 2H, J = 7.26 Hz), 2.87 (s, 3H), 4.02 (t, 2H, J= 6.60 Hz), 4.18 (q, 2H, J= 7.26 Hz), 4.49 (s, 2H), 6.76 (s, IH), 7.26 (m, 2H), 7.44 (d, 2H, J= 8.25 Hz) ¹³C NMR (67.5 MHz, CDC1₃): δ 13.55, 14.70, 18.71, 22.21, 30.37, 30.47, 33.74, 34.27, 39.28, 52.11, 61.59, 66.79, 127.23, 127.59, 129.08, 129.44, 130.50, 133.03, 138.24, 146.18, 150.05, 151.45, 156.53, 157.0 IR (neat, cm^"1): v 2960, 1750, 1678, 1465, 1348, 1222, 1158 MS (ESI⁺): m/z at 511.3 (M⁺ +1)

Anal. Calcd for C₂₄H₃₄N₂0₆S₂: C, 56.5; H, 6.7; N, 5.5; Found: C, 56.9; H, 7.1; N, 5.4

Example 13

N-Butyloxycarbonyl-3-[4-(N-methoxy-N-methylaminomethyl)phenyl]-5- t^'iO-butylthiophene-2-sulfonamide

(a) 3-[4-(N-Methoxy-N-methylaminomethyl)phenyl]-5-.^{' ι}o-butyl-N-tert- butylthiophene-2-sulfonamide

3-(4-Formylphenyl)-5-/-?o-butyl-N-tert-butylthiophene-2-thiophene-2-sulfo- namide (40 mg, 0.11 mmol, see Preparation A above), JV- methoxymethylamine hydrochloride (23.4 mg, 0.22 mmol) and triethylamine (60 μL, 0.44 mmol) were dissolved in dichloroethane charged with molecular sieves (4A, 200 mg) under a Ν₂ atmosphere. NaBH(OAc)₃ (46 mg, 0.22 mmol) was added and the reaction mixture was stirred overnight at ambient temperature. A second portion of the three reagents (1 eq.) were then added and the reaction mixture was stirred overnight. The reaction was quenched with NaHC0₃ (1 mL, satd.) and diluted with ethyl acetate (10 mL). The contents were poured into diatomaceous earth (liquid- liquid extraction cartridge) in a polypropylene column (packed for 1.5 cm, 24 mL size) and eluted with ethyl acetate (30 mL). Concentration under vacuum afforded the crude product, which was then purified using circular chromatography (40% ethyl acetate in petroleum ether).

(b) N-Butyloxycarbonyl-3-[4-(N-methoxy-N-methylaminomethyl phenyl1-5-

.^'_?o-butylthiophene-2-sulfonamide

The product from step (a) was treated in accordance with Steps 3 and 4 of the General Procedure for Examples 1 to 12. The crude product was purified by LCMS (45% to 85% aqueous acetonitrile) to afford pure title product as a colourless solid (36 mg, 79%). mp 101-102°C

1H NMR (270 MHz, CDC1₃): δ 0.87 (t, 3H, J = 7.26 Hz), 0.98 (d, 6H, J =

6.60 Hz), 1.25 (m, 2H), 1.49 (m, 2H), 1.95 (m, IH), 2.64 (s, 3H), 2.71 (d, 2H, J = 6.93 Hz), 3.40 (s, 3H), 3.81 (s, 2H), 4.02 (t, 2H, J = 6.60 Hz), 6.78

(s, IH), 7.43 (m, 4H)

¹³C NMR (67.5 MHz, CDC1₃): δ 13.57, 18.73, 22.23, 30.37, 30.52, 39.30,

44.82, 59.93, 64.22, 66.87, 128.71, 129.45, 130.47, 133.04, 138.14. 146.32.

149.91, 151.47 IR (neat, cm^"1): v 2958, 1751, 1436, 1347, 1159, 1047

MS (ESI⁺): m/z at 469 (M⁺+l)

Anal. Calcd for C₂₂H₃₂N₂0₅S₂: C, 56.4; H, 6.9; N, 6.0; Found: C, 56.8; H,

7.1; N, 5.7 Example 14

N-Butyloxycarbonyl-3-[4-(N-formyl-N-methylaminomethyl^N)phenyll-5-^o- butylthiophene-2-sulfonamide

(a) 3-[4-(N-Formyl-N-methylaminomethyl)phenyll-5-/_ⁱo-butyl-N-tert- butylthiophene-2-sulfonamide

A mixture of 3-[4-(N-methylaminomethyl)phenyl]-5-/^'_O-butyl-N-tert- butylthiophene-2-sulfonamide (25 mg, 0.0063 mmol, prepared according to Step 1 of the General Procedure for Examples 1 to 12, using methylamine as the amine) and ammonium formate (120 mg, 1.9 mmol) in CH₃CΝ (1.5 mL) was refluxed for 12 hours, cooled and evaporated. The residue was directly loaded into a circular chromatography apparatus and eluted with 50% ethyl acetate in petroluem ether to afford the pure sub-title compound as a colourless solid (25 mg, 94%).

(b) N-Butyloxycarbonyl-3-[4-(N-formyl-N-methylaminomethyl)ρhenyl]-5- t^'_.o-butylthiophene-2-sulfonamide

40 mg of 3-[4-(N-formyl-N-methylaminomethyl)phenyl]-5-z5O-butyl-N-tert- butylthiophene-2-sulfonamide (40 mg, 0.12 mmol, see step (a) above) was then treated in accordance with Steps 3 and 4 of the General Procedure for Examples 1 to 12. The crude product was purified by LCMS (35%> to 75% aqueous acetonitrile) to afford pure title product as a colourless syrup (34 mg, 77%). 1H NMR (270 MHz, CDC1₃): δ 0.87 (t, 3H, J = 7.26 Hz), 0.98 (d, 6H, J - 6.60 Hz), 1.24 (m, 2H), 1.50 (m, 2H), 1.94 (m, IH), 2.69 (d, 2H, J = 6.93 Hz), 2.78 & 2.86 (s, 3H), 4.04 (td, 2H, J = 2.31 Hz, J - 6.60 Hz), 4.42 (s, IH), 4.53 (s, IH), 6.76 (d, IH, J = 2.97 Hz), 7.26 (t, 2H, J = 7.92 Hz), 7.47 (t, 2H, J = 7.92 Hz), 8.11 & 8.23 (s, IH), 8.6- 8.9 (br m, IH) ¹³C NMR (67.5 MHz, CDC1₃): δ 13.58, 18.71, 22.20, 29.60, 30.41, 30.47, 34.31, 39.25, 47.55, 53.27, 66.69, 126.79, 127.26, 128.03, 129.29, 129.45, 130.96, 133.53, 133.90, 136.10, 136.26, 145.64, 145.74, 150.42, 151.30, 151.46, 163.06

IR (neat, cm^"1): v 2960, 1746, 1662, 1466, 1346, 1158 MS (ES ): m/z at 467 (MOT) Anal. Calcd for C₂₂H₃oN₂0₅S₂: C, 56.63; H, 6.48; N, 6.0; Found: C, 56.7; H, 6.5; N, 6.0

Example 15

N-Butyloxycarbonyl-3-[4-(N-acetylaminomethyl)phenyl]-5-^o-butylthio- phene-2-sulfonamide

(a) 3- 4-(N-Acetylaminomethyl)phenyll-5- .yo-butyl-N-tert-butylthiophene-

2-sulfonamide

To a nitrogen- flushed solution of Pd(OAc)₂ (17.5 mg, 0.078 mmol) in DME (2 mL), was added triphenylphosphine (82 mg, 0.312 mmol). The solution was flushed with nitrogen again and stirred under a Ν₂ atmosphere for 30 minutes. The brownish suspension was transferred to a nitrogen-flushed mixture of 5-wσ-butyl-2-(N-ter/-butylaminosulfonyl)thiophene-3-boronic acid (0.50 g, 1.56 mmol, see Preparation A, step (c)), N-(4- bromobenzyl)acetamide (0.71 g, 3.12 mmol, prepared form 4- bromobenzylamine and acetyl chloride), and potassium carbonate (0.86 g, 6.24 mmol) in DME:H₂0:EtOH (3.5: 1.5: 1 mL). The mixture was refluxed overnight under a N₂ atmosphere, washed with aqueous NaOH solution (1M), water and brine, dried over anhydrous MgS0₄ and concentrated to afford the residue which was purified by circular chromatography (50% acetone in pet. ether) to afford pure sub-title product as a colourless solid (0.43 g, 65%). mp 171-172°C 1H NMR (270 MHz, CDC1₃): δ 0.97 (d, 6H, J= 6.60 Hz), 1.0 (s, 9H), 1.91 (m, IH), 2.07 (s, 3H), 2.67 (d, 2H, J = 6.93 Hz), 4.47 (d, 2H, J = 5.61 Hz), 6.20 (br s, IH), 6.73 (s, IH), 7.37 (d, 2H, J = 7.92 Hz), 7.53 (d, 2H, J = 7.92 Hz)

¹³C NMR (67.5 MHz, CDC1₃): δ 22.13, 23.21, 29.48, 30.50, 39.16, 43.28, 54.51, 127.76, 128.94, 129.25, 133.88, 136.11, 138.61, 143.14, 148.53, 170.18

IR (neat, cm^"1): v 3314, 2963, 1650, 1535, 1432, 1308, 1136, 1049, 1009, 844

MS (ESI⁺): m/z at 423.1 (M⁺+l)

Anal. Calcd for C₂₁H₃₀N₂O₃S₂: C, 58.44; H, 7.24; N, 6.49; Found: C, 58.1; H, 7.0; N, 6.5

(b) N-Butyloxycarbonyl-3-[4-(N-acetylaminomethyl)phenyll-5-t^'_?o-butyl- thiophene-2-sulfonamide

3-[4-(N-Acetylaminomethyl)phenyl]-5-t^'_y_)-butyl-N-tert-butylthiophene-2- sulfonamide (40 mg, 0.90 mmol, see step (a) above) was treated in accordance with Steps 3 and 4 of the General Procedure of Examples 1 to 12 above. The crude product was purified by LCMS (40% aqueous acetonitrile) to afford the pure title product as a colourless solid (31 mg, 70%). mp 171-173°C

^!H ΝMR (270 MHz, CD₃COCD₃+D₂0): δ 0.84 (t, 3H, J= 7.26 Hz), 0.95 (d, 6H, = 6.60 Hz), 1.24 (m, 2H), 1.46 (m, 2H), 1.87- 2.02 (m, 4H), 2.75 (d, 2H, J = 7.26 Hz), 3.56 (br S, 2H), 3.97 (t, 2H, J = 6.27 Hz), 4.38 (s, 2H), 6.92 (s, IH), 7.32 (d, 2H, J = 8.25 Hz), 7.47 (d, 2H, J - 8.25 Hz) ¹³C ΝMR (67.5 MHz, CD₃COCD₃+D₂0): δ 12.53, 18.04, 21.03, 21.40, 29.86, 38.04, 41.79, 65.21, 126.19, 126.67, 128.52, 129.15, 131.33, 132.22, 139.23, 145.03, 149.78, 150.37, 169.69, 205.98

IR (neat, cm^"1): v 2954, 2496, 1748, 1612, 1458, 1352, 1297, 1150, 1039, 816, 578 MS (ESI⁺): m/z at 467.0 (M⁺+l) Anal. Calcd for C₂₂H₃₀N₂O₅S₂.l/3 H₂0: C, 55.91; H, 6.54; N, 5.93; Found: C, 55.9; H, 6.6; N, 5.9

Example 16 Title compounds of the Examples were tested in Tests A and B above and were found to exhibit an affinity for AT2 receptors of less than Ki = 50 nM and an affinity for ATI receptors of Ki = 1 μM or greater.

Example 17 Title compounds of the Examples are tested in Test C above and are found to stimulate markedly mucosal alkalisation. This effect is blocked by co- administration of the selective AT2 receptor antagonist PD 123319 (Sigma Chemical Company).

Claims

Claims

A compound of formula I,

wherein

X represents -0-, -C(O)- or -S(0)₂-;

R^la and R^lb independently represent H, C_1-6 alkyl, C_1- alkoxy-C_1-6 alkyl, Ar¹, Het¹, C_1-3 alkyl- Ar², C_1-3 alkyl-Het², C_1-3 alkoxy- Ar³ or C_1-3 alkoxy-

Het³; or, in the case where X represents -C(O)- , R^lamay also represent C_1-6 alkoxy or -O-Ar⁴;

Ar¹, Ar², Ar³ and Ar⁴ each independently represent a C₆_ι₀ aryl group, which group is optionally substituted by one or more substituents selected from =0, -OH, cyano, halo, nitro, Cι_-6 alkyl (optionally terminated by

-N(H)C(0)OR^{l la}), .₆ alkoxy, phenyl, -N(R^12a)R^12b, -C(0)R^12c,

-C(0)OR^12d, -C(0)N(R^12e)R^12f, -N(R^12g)C(0)R^12h,

-N(R^12m)S(0)₂R^{l lb},-S(0)_nR^{l lc}, -OS(0)₂R^ll and -S(0)₂N(R¹²ⁿ)R^12p;

1

Het , Het and Het each independently represent a four- to twelve- membered heterocyclic group containing one or more heteroatoms selected from oxygen, nitrogen and/or sulfur, which heterocyclic group is optionally substituted by one or more substituents selected from =0, -OH, cyano, halo, nitro, Cι_₆ alkyl (optionally terminated by -N(H)C(0)OR 11a )., _-6 alkoxy, phenyl, -N(R^12a)R^12b, -C(0)R^12c, -C(0)OR^12d, -C(0)N(R^12e)R^12f, -N(R^12g)C(0)R^12h, -N(R¹²ⁱ)C(0)N(R^12j)R^12k, -N(R^12m)S(0)₂R^{l lb}, -S(0)_nR^{l lc}, -OS(0)₂R^{l ld} and -S(0)₂N(R¹²ⁿ)R^12p; R^lla to R¹¹ independently represent C_1-6 alkyl; R^12a to R^12p independently represent H or C_1-6 alkyl; n represents 0, 1 or 2;

Yi , Y₂, Y₃ and Y₄ independently represent -CH- or -CF-; Zi represents -CH-, -O-, -S-, -N- or -CH=CH-; Z₂ represents -CH-, -0-, -S- or -N-; provided that: (a) Zj and Z₂ are not the same;

(b) when Zi represents -CH=CH-, then Z₂ may only represent -CH- or -N-; and

(c) other than in the specific case in which Zi represents -CH=CH-, and Z₂ represents -CH-, when one of Zi and Z₂ represents -CH-, then the other represents -O- or -S-;

R² represents -S(0)₂N(H)C(0)R⁴, -S(0)₂N(H)S(0)₂R⁴, -C(0)N(H)S(0)₂R⁴, or, when Zi represents -CH=CH-, R² may represent

-N(H)S(0)₂N(H)C(0)R⁵ or -N(H)C(0)N(H)S(0)₂R⁵;

R represents Cι_-6 alkyl, C_1-6 alkoxy or C_1-6 alkoxy-C_1-6-alkyl; R⁴ represents C_1-6 alkyl, C_1-6 alkoxy, C_1-6 alkoxy-C_1-6-alkyl, Cι_-6 alkylamino or di-Cι_₆ alkylamino; and

R⁵ represents C_1-6 alkyl, or a pharmaceutically-acceptable salt thereof.

2. A compound as claimed in Claim 1 wherein R^la and R^lb independently represent H, Cι_₆ alkyl, _-6 alkoxy-C_1-6 alkyl, Ar¹, Het¹, C_1-3 alkyl-Ar², C_1-3 alkyl-Het², Cι_₃ alkoxy-Ar³ or C_1-3 alkoxy-Het³.

3. A compound as claimed in Claim 1 wherein, when X represents -C(O)-, then R^la does not represent -O-Ar⁴.

4. A compound as claimed in Claim 1 or Claim 2 wherein R la

represents H, Cι_-5 alkoxy, Cι_₅ alkyl, Ar¹ or Het¹.

A compound as claimed in Claim 4, wherein R^la represents H, Cι_₃ alkoxy, C_1-4 alkyl, phenyl or thiophenyl.

6. A compound as claimed in any one of the preceding claims, wherein R^l represents H, .₄ alkyl, phenyl (optionally substituted by one or more C_J-2 alkyl groups), C_1-2 alkylphenyl, C_1-2 alkyl-Het or C_1-2 alkoxy-C_1-2- alkyl.

1 K

7. A compound as claimed in Claim 6, wherein R represents H, C_1- alkyl, phenyl (optionally substituted by one or more methyl groups), benzyl or methylpyridinyl.

8. A compound as claimed in any one of the preceding claims, wherein X represents -O- or -C(O)-.

9. A compound as claimed in any one of the preceding claims, wherein Yi, Y₂, Y₃ and Y₄ all represent -CH-.

10. A compound as claimed in any one of the preceding claims, wherein Zi represents -S- or -CH=CH-.

11. A compound as claimed in Claim 10, wherein Zi represents -S-.

12. A compound as claimed in any one of the preceding claims, wherein Z₂ represents -CH-.

13. A compound as claimed in any one of the preceding claims, wherein R³ represents n-butyl or _s o-butyl.

14. A compound as claimed in any one of the preceding claims, wherein, when R² represents S(0)₂N(H)C(0)R⁴, -S(0)₂N(H)S(0)₂R⁴ or

-C(O)N(H)S(0)₂R⁴, R⁴ represents n-butyl, n-butoxymethyl, wo-butoxy or n- butoxy.

15. A compound as claimed in any one of the preceding claims, wherein R² represents -S(0)₂N(H)C(0)R⁴.

16. A compound as claimed in Claim 15 wherein R represents n- butoxymethyl, wo-butoxy or n-butoxy.

17. A compound as claimed in Claim 1, which is:

N-butyloxycarbonyl-3-[4-(N-acetyl-N-benzylaminomethyl)phenyl]-5-t.s^,o- butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-[4-(N-benzylpentylamidomethyl)phenyl]-5-wo- butylthiophene-2-sulfonamide; N-butyloxycarbonyl-3-[4-(N-/?-tolylbenzylamidomethyl)phenyl]-5-wo- butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-[4-(N-acetyl-N-p-tolylaminomethyl)phenyl]-5-wo- butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-{4-[N-(pyridin-3-ylmethyl)benzylamidomethyl]- phenyl} -5-iso -butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-[4-(N-acetyl-N-pyridin-3-ylmethylaminomethyl)- phenyl]-5-.^'_rø-butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-[4-(Ν-methylpentylamidomethyl)phenyl]-5-iso- butylthiophene-2-sulfonamide; N-butyloxycarbonyl-3-[4-(N-acetyl-N-methylaminomethyl)ρhenyl]-5-wo- butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-[4-(N-ethyl-thioρhenecarbonylaminomethyl)- phenyl]-5-z^'_?o-butylthiophene-2-sulfonamide; N-butyloxycarbonyl-3-[4-(N-acetyl-N-ethylaminomethyl)phenyl]-5-f_?_)- butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-[4-(N-methanesulfonyl-N-methylaminomethyl)- phenyl]-5-t5O-butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3 - {4- [N-(ethyloxycarbonyl)-N-me thylaminome thyl] - phenyl} -5-.^'sø-butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-[4-(N-methoxy-N-methylaminomethyl)phenyl]-5-t^'_sO- butylthiophene-2-sulfonamide;

N-butyloxycarbonyl-3-[4-(N-formyl-N-methylaminomethyl)phenyl]-5-wo- butylthiophene-2-sulfonamide; or N-butyloxycarbonyl-3-[4-(N-acetylaminomethyl)phenyl]-5-wo-butylthio- phene-2-sulfonamide.

18. A pharmaceutical formulation including a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, in admixture with a pharmaceutically acceptable adjuvant, diluent or carrier.

19. A compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for use as a pharmaceutical.

20. A compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for use in the treatment of a condition in which selective agonism of the AT2 receptor is desired and/or required.

21. A compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for use in the treatment of a condition in which endogenous production of Angll is deficient.

22. A compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for use in the treatment of a condition in which an increase in the effect of Angll is desired or required.

23. A compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for use in the treatment of a condition where AT2 receptors are expressed and their stimulation is desired or required.

24. The use of a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for the treatment of a condition in which selective agonism of the AT2 receptor is desired and/or required.

25. The use of a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for the treatment of a condition in which endogenous production of Angll is deficient.

26. The use of a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for the treatment of a condition in which an increase in the effect of Angll is desired or required.

27. The use of a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for the treatment of a condition where AT2 receptors are expressed and their stimulation is desired or required.

28. The use as claimed in any one of Claims 24 to 27, wherein the condition is of the gastrointestinal tract, the cardiovascular system, the respiratory tract, the kidneys, the eyes, the female reproductive (ovulation) system, or the central nervous system.

29. The use as claimed in Claim 28, wherein the condition is oesophagitis, Barrett's oesophagus, a gastric ulcer, a duodenal ulcer, dyspepsia (including non-ulcer dyspepsia), gastro-oesophageal reflux, irritable bowel syndrome, inflammatory bowel disease, pancreatitis, hepatic disorders (including hepatitis), gall bladder disease, multiple organ failure, sepsis, xerostomia, gastritis, gastroparesis, hyperacidity, a disorder of the bilary tract, coelicia, Crohn's disease, ulcerative colitis, diarrhoea, constipation, colic, dysphagia, vomiting, nausea, indigestion, Sjδgren's syndrome, inflammatory disorders, asthma, an obstructive lung disease (including chronic obstructive lung disease), pneumonitis, pulmonary hypertension, adult respiratory distress syndrome, renal failure, nephritis, renal hypertension, diabetic retinopathy, premature retinopathy, retinal microvascularisation, ovulatory dysfunction, hypertension, cardiac hypertrophy, cardiac failure, artherosclerosis, arterial thrombosis, venous thrombosis, endothelial dysfunction, endothelial lesions, post baloon dilatation stenosis, angiogenesis, diabetic complications, microvascular dysfunction, angina, cardiac arrhythmias, claudicatio intermittens, preeclampsia, myocardial infarction, reinfarction, ischaemic lesions, erectile dysfunction, neointima proliferation, cognitive dysfunctions, dysfunctions of food intake (hunger/satiety), thirst, stroke, cerebral bleeding, cerebral embolus, cerebral infarction, hypertrophic disorders, prostate hypeφlasia, autoimmune disorders, psoriasis, obesity, neuronal regeneration, an ulcer, adipose tissue hypeφlasia, stem cell differentiation and proliferation, cancer, apoptosis, tumours, hypertrophy diabetes, neuronal lesions or organ rejection.

30. The use as claimed in Claim 29, wherein the condition is non-ulcer dyspepsia, irritable bowel syndrome, multiple organ failure, hypertension or cardiac failure.

31. A method of treatment of a condition in which selective agonism of the AT2 receptor is desired and/or required, which method comprises administration of a therapeutically effective amount of a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, to a person suffering from, or susceptible to, such a condition.

32. A pharmaceutical formulation including a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, and an

ATI receptor antagonist, in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier.

33. A kit of parts comprising components: (a) a pharmaceutical formulation including a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier; and (b) a pharmaceutical formulation including an ATI receptor antagonist, in admixture with a pharmaceutically- acceptable adjuvant, diluent or carrier, which components (a) and (b) are each provided in a form that is suitable for administration in conjunction with the other.

34. A pharmaceutical formulation including a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, and an angiotensin converting enzyme inhibitor, in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier.

35. A kit of parts comprising components :

(a) a pharmaceutical formulation including a compound as defined in any one of Claims 1 to 17, or a pharmaceutically acceptable salt thereof, in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier; and

(b) a pharmaceutical formulation including an angiotensin converting enzyme inhibitor, in admixture with a pharmaceutically-acceptable adjuvant, diluent or carrier, which components (a) and (b) are each provided in a form that is suitable for administration in conjunction with the other.

36. A process for the preparation of a compound as defined in Claim 1, which comprises:

(i) for compounds of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ or -S(0)₂N(H)S(0)₂R⁴, and R⁴ is as defined in Claim 1, reaction of a compound of formula II,

wherein R^la, R^lb, X, Y_1} Y₂, Y₃, Y₄, Z Z₂ and R³ are as defined in Claim 1 with a compound of formula III, R⁴GL ^l III wherein G represents C(O) or S(0)₂ (as appropriate), L¹ represents a suitable leaving group and R is as defined in Claim 1 ; (ii) for compounds of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ and R⁴ represents C_1-6 alkoxy-C_1-6-alkyl, coupling of a compound of formula II as defined above with a compound of formula IV,

R^4aC0₂H IV wherein R^4a represents C_1-6 alkoxy-Cι_-6-alkyl;

9 Δ

(iii) for compounds of formula I in which R represents -C(0)N(H)S(0)₂R and R⁴ is as defined in Claim 1, coupling of a compound of formula V,

wherein R^la, R^lb, X, Y_b Y₂, Y₃, Y₄, Z,, Z₂ and R³ are as defined in Claim 1 with a compound of formula VI,

R⁴S(0)₂NH₂ VI wherein R⁴ is as defined in Claim 1;

(iv) for compounds of formula I in which R² represents -C(0)N(H)S(0)₂R⁴ and R⁴ is as defined in Claim 1, coupling of a compound of formula VII,

wherein R^la, R^lb, X, Y Y₂, Y₃, Y₄, Z Z₂ and R³ are as defined in Claim 1 with a compound of formula VIII,

R⁴S(0)₂C1 VIII wherein R⁴ is as defined in Claim 1;

(v) for compounds of formula I in which R² represents -N(H)S(0)₂N(H)C(0)R⁵ and R⁵ is as defined in Claim 1, reaction of a compound of formula IX,

wherein R^la, R^lb, X, Y_u Y₂, Y₃, Y₄, Z Z₂ and R³ are as defined in Claim 1 with a compound of formula X

R⁵C(0)N(H)S(0)₂C1 X wherein R⁵ is as defined in Claim 1;

(vi) for compounds of formula I in which R² represents

-N(H)C(0)N(H)S(0)₂R⁵ and R⁵ is as defined in Claim 1, reaction of a compound of formula IX as defined above with a compound of formula XI,

R⁵S(0)₂N(H)C(0)OR^x XI wherein R^x represents Cι_₂ alkyl and R⁵ is as defined in Claim 1;

(vii) for compounds of formula I in which R² represents

-N(H)C(0)N(H)S(0)₂R⁵ and R⁵ is as defined in Claim 1, reaction of a compound of formula IX as defined above with a compound of formula

XII,

R⁵S(0)₂NCO XII wherein R⁵ is as defined in Claim 1; (viii) for compounds of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ and R⁴ represents Cι„₆ alkylamino, reaction of a compound of formula II as defined above with a compound of formula XIII,

R^4bNCO XIII wherein R^4b is Ci_₆ alkyl;

(ix) for compounds of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ and R represents di-Ci_-6 alkylamino, reaction of a corresponding compound of formula I in which R² represents -S(0)₂N(H)C(0)R⁴ and R⁴ represents C_1- alkoxy with a compound of formula Xllla, R^4cN(H)R^4d Xllla wherein R^4c and R^4d independently represent Cι_-6 alkyl; or (x) for compounds of formula I in which X represents O, reductive amination of a compound of formula XIV,

wherein Y_l3 Y₂, Y₃, Y₄, Z_1? Z₂, R and R^ are as defined in Claim 1, in the presence of a compound of formula XV,

R^laONHR^lb XV wherein R^la and R^lb are as defined in Claim 1.

37. A compound of formula II as defined in Claim 36 or a protected derivative thereof, provided that when Zi represents -CH=CH-, then Z₂ does not represent -CH-.

38. A compound of formula II as defined in Claim 36, or a protected derivative thereof, wherein Zi represents -S- and Z₂ represents -CH-.

39. A compound of formula V as defined in Claim 36 or a protected derivative thereof.

40. A compound of formula VII as defined in Claim 36 or a protected derivative thereof.

41. A compound of formula IX as defined in Claim 36 or a protected derivative thereof.