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WO2004081514A2 - Dispositif et procede de transfert cellulaire in vitro - Google Patents

Dispositif et procede de transfert cellulaire in vitro Download PDF

Info

Publication number
WO2004081514A2
WO2004081514A2 PCT/DE2004/000492 DE2004000492W WO2004081514A2 WO 2004081514 A2 WO2004081514 A2 WO 2004081514A2 DE 2004000492 W DE2004000492 W DE 2004000492W WO 2004081514 A2 WO2004081514 A2 WO 2004081514A2
Authority
WO
WIPO (PCT)
Prior art keywords
biological material
carrier means
removal
carrier
removal part
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2004/000492
Other languages
German (de)
English (en)
Other versions
WO2004081514A3 (fr
Inventor
Karl Skriner
Veit Krenn
Reinhold SCHÄFER
Manfred Dietel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Charite Universitaetsmedizin Berlin
Original Assignee
Charite Universitaetsmedizin Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Charite Universitaetsmedizin Berlin filed Critical Charite Universitaetsmedizin Berlin
Publication of WO2004081514A2 publication Critical patent/WO2004081514A2/fr
Publication of WO2004081514A3 publication Critical patent/WO2004081514A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
    • B01L3/5055—Hinged, e.g. opposable surfaces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/0096—Casings for storing test samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Instruments for taking body samples for diagnostic purposes; Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N2001/028—Sampling from a surface, swabbing, vaporising

Definitions

  • the invention relates to a device for in vivo cell transfer according to the preamble of claim 1 and a method according to the preamble of claim 16.
  • a smear is the removal of test material from skin and mucosal surfaces or wounds for cytological, microbiological and / or molecular biological examination.
  • Conventional smears are obtained by removing biological material, in particular cells from skin and mucous membrane surfaces or wounds, by mechanical abrasion. This is usually done using sterile cotton swabs.
  • Cotton swabs are removed for diagnostic access.
  • the cells are placed, for example, in a suitable nutrient medium or a reaction solution so that they can only be examined in the following step.
  • Biopsy and curettage represent further possibilities for obtaining cells.
  • a biopsy here means the taking of a tissue sample by puncturing with a hollow needle.
  • a curettage is an excavation. The disadvantage of both methods is their invasive nature.
  • Storage is understood to mean both the cultivation or cultivation of living material and the fixation and staining of organic material using the known methods.
  • the therapy also encompasses the therapy of biological material on the carrier.
  • the invention relates to a device for the application, removal and / or storage of biological material.
  • a carrier material is provided for the cell transfer that enables the reversible immobilization of biological material for a predetermined duration, in particular for the duration of a diagnosis, therapy and / or until the application of the biological material.
  • a device which enables biological material to be obtained from a biological body with minimal invasiveness. At the same time, the integrity of the woven association is preserved; thus there is a situation close to in vivo.
  • the biological material after a Predeterminable duration can be removed from the carrier in a targeted manner.
  • a predetermined duration is understood to mean the period of time for which the biological material should expediently remain on the device according to the invention until the diagnosis and / or the therapy and / or the breeding has been carried out and the biological material has possibly been fed (applied) to a biological body ) becomes.
  • the period of obilization can be a few days.
  • the device according to the invention can be easily sterilized and, after being produced as a single-use article, can be discarded after use.
  • Carrier material at least partially a coating with at least one biologically active substances, in particular with a nucleic acid, a peptide, a protein, a lipid, a hormone, a growth factor, a vitamin, a low molecular weight compound, a co-factor and / or a drug.
  • the biological material can be selectively bound to the device according to the invention, for example via the interaction of certain receptors with their ligands.
  • Therapeutically active substances can also be added to the biological material immediately upon removal.
  • Biologically active substances are understood to mean all substances which interact with biological substances.
  • the device has at least one nutrient medium for growing biological material.
  • the nutrient medium can be associated with the biological material and thus allows the
  • the removed biological material can be kept alive after being removed from the biological body and possibly increased or propagated.
  • the device has at least one chip for the biochemical examination of the biological material.
  • the device preferably also has at least one hinge means. By folding over along the axis predetermined by the hinge means, the removed biological material z. B. be brought into contact with a chip or a nutrient medium. This allows z. B. to carry out an immediate diagnosis of the material with the help of the chip or to grow the material immediately after the removal under suitable environmental conditions.
  • a chip is to be understood as a diagnostic aid on which so-called “probes” are applied in a defined manner, which interact with the material to be examined. These interactions generate signals that can be measured and evaluated with suitable equipment "Probes” can be z.
  • Probes can be z.
  • the carrier comprises nitrocellulose, nylon, plastic and / or coated glass, in particular glass coated with nitrocellulose, or consists of nitrocellulose, nylon, plastic and / or coated glass.
  • the carrier can consist of a number of different materials, the only requirement being that the cells can adhere to it in a suitable manner.
  • Other common carrier materials are polystyrene, polycarbonate, polytetrafluoroethylene (PTFE), cellophane, polyacrylamides, stainless steel, palladium and titanium. It is also possible to coat various surfaces with materials, such as Polylysine, fibronectin, collagen or gelatin. Coating of the immobilization agent with agar is also possible.
  • the device has at least one removal and / or at least one handle part, the at least one removal part preferably being designed as a slide and the at least one removal part being particularly preferably detachable from the handle part along a predetermined breaking point.
  • the biological material obtained can be examined quickly and conveniently immediately after removal.
  • microscopic examination is particularly suitable.
  • the at least one removal part is essentially rod-shaped.
  • the at least one removal part advantageously has a rounded and / or an essentially punctiform end.
  • the at least one grip part and / or the at least one removal part is arched.
  • the various forms of the device according to the invention allow the (veterinary) doctor to remove biological material from various parts of the body under various conditions.
  • Body part is understood here to mean any part of a living or dead biological organism.
  • edges and corners of the device according to the invention are advantageously rounded. This reduces the risk of injuring the patient while extracting material.
  • the biological material on the carrier is predominantly in the form of a single layer (monolayer). A monolayer is advantageous for many diagnostic procedures, such as microscopy.
  • the carrier can be inserted into an apparatus with which the polymerase chain reaction (PCR) can be carried out (PCR machine).
  • PCR polymerase chain reaction
  • the biological material is removed from at least one part of the body, in particular skin and / or mucous membrane surfaces.
  • the biological material adhering to the carrier is incubated with at least one biologically active substance, in particular a nucleic acid, a peptide, a protein, a lipid, a hormone, a growth factor, a vitamin, a low molecular weight compound, a co Factor and / or a drug.
  • at least one biologically active substance in particular a nucleic acid, a peptide, a protein, a lipid, a hormone, a growth factor, a vitamin, a low molecular weight compound, a co Factor and / or a drug.
  • the biological material is applied to at least one part of the body, in particular skin and / or mucous membrane surfaces.
  • the biological material is grown directly on the device. This eliminates the need for those obtained from the biological body First having to transfer cells into a medium suitable for the subsequent application.
  • At least one suitable solid or gel-like nutrient medium is brought into contact with the biological material.
  • the device according to the invention can then be used with suitable environmental parameters, such as temperature, atmospheric humidity and CO 2 partial pressure for propagation or storage, of biological material.
  • the biological material adhering to the at least one carrier is advantageously used in the method in at least one diagnostic method.
  • the cells adhering to the at least one carrier are examined histopathologically, immunohistochemically, genetically and / or molecularly.
  • the cells are advantageously incubated with at least one biologically active substance, in particular a nucleic acid, a peptide, a protein, a lipid, a hormone, a growth factor, a vitamin, a low molecular weight compound, a co-factor and / or a medicament.
  • at least one biologically active substance in particular a nucleic acid, a peptide, a protein, a lipid, a hormone, a growth factor, a vitamin, a low molecular weight compound, a co-factor and / or a medicament.
  • the biological material adhering to the at least one carrier means (14a; 14b; 14c; 214a; 214b; 214c) is used in at least one therapeutic method.
  • ⁇ such as incubation with drugs, gene therapy transfection with nucleic acids, for example by lipofection or bombardment with a “gene gun ,” .
  • the first spatula 10a shows a first spatula 10a on a scale of 1: 1 as an embodiment of the device according to the invention.
  • the first spatula 10a consists of a first handle part 11a and a first removal part 12a.
  • the first removal part 12a has a first carrier means 14a for the reversible immobilization of biological material, which enables biological material, in particular cells, to adhere to the spatula 10a.
  • this first carrier 14a is nitrocellulose, which is arranged on the first spatula 10a.
  • the carrier material 14a can also contain nitrocellulose in addition to other components (e.g. in a matrix).
  • the first removal part 12a is simultaneously designed as a specimen slide 12a.
  • first handle part 11a there is a first predetermined breaking point 13a, which enables the first handle part 11a to be separated after the biological material has been obtained.
  • the first handle part 11a can be separated and the material obtained can be immediately examined microscopically without the material having to be transferred from the device to a separate slide.
  • the separation of the first handle part 11a improves the manageability when carrying out further (diagnostic) methods, such as, for example, enzyme histochemical, immunohistochemical and molecular-biological methods (for example in situ hybridization).
  • diagnostic methods such as, for example, enzyme histochemical, immunohistochemical and molecular-biological methods (for example in situ hybridization).
  • the edges 15a of the first spatula 10a are rounded in order to minimize the risk of injury to the patient.
  • the first spatula 10a is made of a plastic that is easy to sterilize and can be discarded after use as a disposable item.
  • a device that is easy to handle and minimally invasive in everyday clinical practice is available for the application, removal and / or storage of biological material.
  • FIG. 1b shows a second spatula 10b, which is derived from the first embodiment, so that reference can be made to the above description.
  • the second spatula 10b additionally has a nutrient medium 16b.
  • the nutrient medium 16b is brought into contact with the biological medium on a second carrier means 14b by folding over a first hinge means 17b
  • the biological material can thus be cultivated over a longer period of time, in order finally to be available for diagnosis and / or therapy and / or application in a biological body.
  • FIG. 1c shows a third embodiment of the device 10c according to the invention.
  • This has a chip 18c.
  • the chip 18c is brought into contact with the biological material located on a third carrier means 14c by folding over a second hinge means 17c.
  • the chip 18c thus enables an immediate biochemical examination of the biological material obtained on the device 10c according to the invention.
  • Fig. 2a shows a spatula, in which the fourth handle part 211a is elongated to z. B. to enable an operator to obtain material during an operation in poorly accessible regions.
  • the fourth handle part 211a is elongated to z. B. to enable an operator to obtain material during an operation in poorly accessible regions.
  • Carrier means 214a has a cylindrical shape with rounded edges 215a and can be separated from fourth handle part 211a along fourth predetermined breaking point 213a. For reliable sampling, this has to be done Carrier means have a minimum area of several square millimeters.
  • a fifth carrier means 214b is “point-shaped”.
  • FIG. 2c shows a further embodiment of the device according to the invention, in which both a fifth handle 211c and a sixth support means 214c are designed in an arc shape. This embodiment is also intended to enable a surgeon to obtain material in regions that are difficult to access, for example during an operation in a body cavity.
  • the sixth carrier means 214c can be separated from the fifth handle 211c along the fifth predetermined breaking point 213c.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Medical Informatics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)

Abstract

La présente invention concerne un dispositif pour appliquer, prélever et/ou conserver de la matière biologique, comprenant au moins un système de support (14a; 14b; 14c; 214a; 214b; 214c) permettant l'immobilisation réversible de la matière biologique pendant une durée déterminée, notamment pendant un temps de diagnostic, de thérapie et/ou jusqu'à application de la matière biologique. L'invention permet d'obtenir un dispositif et un procédé qui, lors du prélèvement de cellules pour diagnostic ultérieur et thérapie, s'avèrent au minimum invasifs et garantissent la conservation de l'intégrité des tissus, la matière biologique étant accessible d'un point de vue diagnostic et/ou thérapeutique directement grâce au dispositif de l'invention.
PCT/DE2004/000492 2003-03-12 2004-03-08 Dispositif et procede de transfert cellulaire in vitro Ceased WO2004081514A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10311981.7 2003-03-12
DE2003111981 DE10311981A1 (de) 2003-03-12 2003-03-12 Vorrichtung und Verfahren für den vivo-Zelltransfer

Publications (2)

Publication Number Publication Date
WO2004081514A2 true WO2004081514A2 (fr) 2004-09-23
WO2004081514A3 WO2004081514A3 (fr) 2004-11-18

Family

ID=32892301

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2004/000492 Ceased WO2004081514A2 (fr) 2003-03-12 2004-03-08 Dispositif et procede de transfert cellulaire in vitro

Country Status (2)

Country Link
DE (1) DE10311981A1 (fr)
WO (1) WO2004081514A2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2347719A1 (fr) * 2010-01-22 2011-07-27 Labor Diagnostika Nord GmbH & Co. KG Dispositif et kit pour recueillir les fluides corporels
US8304237B2 (en) 2007-06-20 2012-11-06 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. Method and device for forming biologic cell aggregates
US8632733B2 (en) 2010-01-22 2014-01-21 Labor Diagnostika Nord Gmbh & Co. Kg Device and kit for collecting body fluids
JP2020529606A (ja) * 2017-08-03 2020-10-08 フィブロテックス エー スキンケアに適用するための側方流動分析及び装置

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
DE102007036853B4 (de) * 2007-08-06 2014-02-13 Ralf Günther Vorrichtung zum Auflegen und zur Manipulation von Geweben
GB202301203D0 (en) * 2023-01-27 2023-03-15 Morrama Ltd A component for use in a diagnostic testing device

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8304237B2 (en) 2007-06-20 2012-11-06 Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. Method and device for forming biologic cell aggregates
EP2347719A1 (fr) * 2010-01-22 2011-07-27 Labor Diagnostika Nord GmbH & Co. KG Dispositif et kit pour recueillir les fluides corporels
US8632733B2 (en) 2010-01-22 2014-01-21 Labor Diagnostika Nord Gmbh & Co. Kg Device and kit for collecting body fluids
JP2020529606A (ja) * 2017-08-03 2020-10-08 フィブロテックス エー スキンケアに適用するための側方流動分析及び装置

Also Published As

Publication number Publication date
WO2004081514A3 (fr) 2004-11-18
DE10311981A1 (de) 2004-09-23

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