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WO2002037077A3 - Techniques de criblage reposant sur un systeme d'analyse de regulation transcriptionnelle au moyen de lignees cellulaires transformees - Google Patents

Techniques de criblage reposant sur un systeme d'analyse de regulation transcriptionnelle au moyen de lignees cellulaires transformees Download PDF

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Publication number
WO2002037077A3
WO2002037077A3 PCT/KR2001/001881 KR0101881W WO0237077A3 WO 2002037077 A3 WO2002037077 A3 WO 2002037077A3 KR 0101881 W KR0101881 W KR 0101881W WO 0237077 A3 WO0237077 A3 WO 0237077A3
Authority
WO
WIPO (PCT)
Prior art keywords
screening methods
cell lines
transformed cell
analysis system
methods based
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2001/001881
Other languages
English (en)
Other versions
WO2002037077A2 (fr
Inventor
Seok-Geun Lee
Mun-Seog Chang
Ho-Jin Shin
Hyune-Mo Rho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MEDEXBIO CO
Original Assignee
MEDEXBIO CO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MEDEXBIO CO filed Critical MEDEXBIO CO
Priority to AU2002215239A priority Critical patent/AU2002215239A1/en
Publication of WO2002037077A2 publication Critical patent/WO2002037077A2/fr
Publication of WO2002037077A3 publication Critical patent/WO2002037077A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne des techniques de criblage permettant d'évaluer l'activité de facteurs ou de promoteurs de transcription au moyen de lignées cellulaires transformées. Plus précisément, cette invention concerne (a) des procédés de criblage propres à détecter des substances ayant une incidence sur l'activité de transcription au moyen de lignées cellulaires transformées et qui permettent d'obtenir des données sur les cellules avec peu d'effets artificiels, (b) des lignées cellulaires utilisées pour les techniques de criblage et (c) des techniques de criblage débouchant sur la mise au point de nouveaux médicaments à partir de composés.
PCT/KR2001/001881 2000-11-06 2001-11-06 Techniques de criblage reposant sur un systeme d'analyse de regulation transcriptionnelle au moyen de lignees cellulaires transformees Ceased WO2002037077A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002215239A AU2002215239A1 (en) 2000-11-06 2001-11-06 Screening methods based on transcriptional regulation analysis system using transformed cell lines

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR2000/65656 2000-11-06
KR1020000065656A KR20020035393A (ko) 2000-11-06 2000-11-06 전사조절 인자 또는 프로모터의 활성도 측정용 리포터벡터및 그에 의해 형질전환된 세포주

Publications (2)

Publication Number Publication Date
WO2002037077A2 WO2002037077A2 (fr) 2002-05-10
WO2002037077A3 true WO2002037077A3 (fr) 2002-11-07

Family

ID=19697487

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2001/001881 Ceased WO2002037077A2 (fr) 2000-11-06 2001-11-06 Techniques de criblage reposant sur un systeme d'analyse de regulation transcriptionnelle au moyen de lignees cellulaires transformees

Country Status (3)

Country Link
KR (2) KR20020035393A (fr)
AU (1) AU2002215239A1 (fr)
WO (1) WO2002037077A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10987362B2 (en) 2004-03-12 2021-04-27 Intercept Pharmaceuticals, Inc. Treatment of fibrosis using FXR ligands
KR100711939B1 (ko) * 2005-12-29 2007-05-02 한국화학연구원 형질전환 세포주 및 재조합 바이러스를 이용한 스크리닝방법
KR100783491B1 (ko) * 2006-07-27 2007-12-07 고려대학교 산학협력단 프로모터 활성 측정용 벡터, 이를 이용한 프로모터 활성측정방법 및 상기 방법에 의해 선별된 프로모터
KR101144885B1 (ko) * 2007-11-09 2012-06-13 포항공과대학교 산학협력단 에스트로겐, 다이옥신, 및 이들의 유사물질 검출용 이중바이오센서 및 이의 용도
KR101428566B1 (ko) * 2012-04-09 2014-08-11 한국생명공학연구원 프로모터 강도 또는 목적 단백질의 발현 양을 검출하기 위한 재조합 형광 번역 리포터 벡터 및 이의 용도
CN103305550B (zh) * 2013-04-10 2015-06-17 重庆医科大学附属儿童医院 一种检测AP2α转录因子活性的报告基因载体
CN110885845A (zh) * 2019-06-19 2020-03-17 中国农业科学院农业基因组研究所 一种基于随机序列利用载体筛选活性增强子的方法
CN110257430A (zh) * 2019-06-19 2019-09-20 中国农业科学院农业基因组研究所 一种活性增强子筛选载体及其构建方法
CN116286991B (zh) * 2023-02-10 2023-10-13 中国农业科学院农业基因组研究所 全基因组增强子筛选系统、筛选方法及应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997010360A1 (fr) * 1995-09-13 1997-03-20 Chiron Corporation Procede et produit d'assemblage pour la recherche d'inhibiteurs d'activation de la transcription
WO1998003676A1 (fr) * 1996-07-17 1998-01-29 Hagenmaier Hans Paul Procede pour detecter des effecteurs d'interactions proteine-proteine intracellulaires et/ou intercellulaires
US5976793A (en) * 1989-07-18 1999-11-02 Oncogene Science, Inc. Methods of transcriptionally modulating gene expression and of discovering chemicals capable as gene expression modulators
WO2000040720A1 (fr) * 1999-01-08 2000-07-13 Sumitomo Chemical Company, Limited Cellules permettant de mesurer la capacite a promouvoir la transcription

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976793A (en) * 1989-07-18 1999-11-02 Oncogene Science, Inc. Methods of transcriptionally modulating gene expression and of discovering chemicals capable as gene expression modulators
WO1997010360A1 (fr) * 1995-09-13 1997-03-20 Chiron Corporation Procede et produit d'assemblage pour la recherche d'inhibiteurs d'activation de la transcription
WO1998003676A1 (fr) * 1996-07-17 1998-01-29 Hagenmaier Hans Paul Procede pour detecter des effecteurs d'interactions proteine-proteine intracellulaires et/ou intercellulaires
WO2000040720A1 (fr) * 1999-01-08 2000-07-13 Sumitomo Chemical Company, Limited Cellules permettant de mesurer la capacite a promouvoir la transcription

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 200041, Derwent World Patents Index; AN 2000-475830/41 *

Also Published As

Publication number Publication date
WO2002037077A2 (fr) 2002-05-10
KR20030065506A (ko) 2003-08-06
KR20020035393A (ko) 2002-05-11
AU2002215239A1 (en) 2002-05-15

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