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WO2002018642A1 - Procede de detection de gene - Google Patents

Procede de detection de gene Download PDF

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Publication number
WO2002018642A1
WO2002018642A1 PCT/JP2001/007020 JP0107020W WO0218642A1 WO 2002018642 A1 WO2002018642 A1 WO 2002018642A1 JP 0107020 W JP0107020 W JP 0107020W WO 0218642 A1 WO0218642 A1 WO 0218642A1
Authority
WO
WIPO (PCT)
Prior art keywords
gene
probe
polymer
detected
point
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2001/007020
Other languages
English (en)
Japanese (ja)
Inventor
Mitsugu Usui
Mari Mitsuka
Chikako Hakii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanko Junyaku Co Ltd
Original Assignee
Sanko Junyaku Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanko Junyaku Co Ltd filed Critical Sanko Junyaku Co Ltd
Priority to KR1020027002719A priority Critical patent/KR20020065471A/ko
Priority to CA002389310A priority patent/CA2389310A1/fr
Priority to AU78730/01A priority patent/AU7873001A/en
Priority to EP01956887A priority patent/EP1229131A1/fr
Publication of WO2002018642A1 publication Critical patent/WO2002018642A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de détection de gène, au moyen duquel un gène peut être détecté par formation d'un polymère grâce au procédé PALSAR sans capture des oligonucléotides ligaturés ni lavage des oligonucléotides en excès. En utilisant plusieurs paires de sondes constituées de n (n≥3) zones de séquence de base mutuellement complémentaires, un point d'un côté du domaine complémentaire, un point de chaque côté de celui-ci ou deux ou plusieurs points d'un gène cible de la paire de sondes précitée sont préalablement clivés, puis une réaction d'hybridation, une réaction de ligature et une réaction de dissociation sont effectuées à température contrôlée. Ainsi, les sondes clivées sont ligaturées afin de former une sonde entière. En utilisant un procédé de construction d'un polymère de sonde, un polymère bicaténaire est formé et le gène précité est détecté.
PCT/JP2001/007020 2000-08-30 2001-08-14 Procede de detection de gene Ceased WO2002018642A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1020027002719A KR20020065471A (ko) 2000-08-30 2001-08-14 유전자의 검출방법
CA002389310A CA2389310A1 (fr) 2000-08-30 2001-08-14 Procede de detection de gene
AU78730/01A AU7873001A (en) 2000-08-30 2001-08-14 Method of detecting gene
EP01956887A EP1229131A1 (fr) 2000-08-30 2001-08-14 Procede de detection de gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000-261687 2000-08-30
JP2000261687 2000-08-30

Publications (1)

Publication Number Publication Date
WO2002018642A1 true WO2002018642A1 (fr) 2002-03-07

Family

ID=18749488

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/007020 Ceased WO2002018642A1 (fr) 2000-08-30 2001-08-14 Procede de detection de gene

Country Status (7)

Country Link
US (1) US20030175689A1 (fr)
EP (1) EP1229131A1 (fr)
KR (1) KR20020065471A (fr)
CN (1) CN1388831A (fr)
AU (1) AU7873001A (fr)
CA (1) CA2389310A1 (fr)
WO (1) WO2002018642A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004072302A1 (fr) * 2003-02-14 2004-08-26 Eisai Co., Ltd. Procede d'amplification de signaux destine a la detection du gene exprime
WO2007037282A1 (fr) * 2005-09-27 2007-04-05 Eisai R & D Management Co., Ltd. Procédé de formation d'un autoagrégat sur une microparticule et procédé de détection d'un analyte cible
WO2007108378A1 (fr) 2006-03-15 2007-09-27 Eisai R & D Management Co., Ltd. Procédé de formation d'un polymère de sondes de signal
US7632639B2 (en) 2003-02-21 2009-12-15 Eisai R&D Management Co., Ltd. Signal amplification method for detecting mutated gene
EP2202319A4 (fr) * 2007-10-24 2010-10-20 Eisai R&D Man Co Ltd Sonde à acide nucléique et procédé de production d'un polymère pour sonde

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2447332A1 (fr) * 2001-09-28 2003-04-10 Sanko Junyaku Co., Ltd. Procede d'amplification des signaux de puces adn
CA2564838A1 (fr) * 2004-04-28 2005-11-10 Eisai R&D Management Co., Ltd. Procede d'hybridisation
ES2650066T3 (es) * 2008-05-28 2018-01-16 Wako Pure Chemical Industries, Ltd. Cebador y sonda para la detección de Mycobacterium intracellulare, y método para la detección de Mycobacterium intracellulare empleando el cebador o la sonda
US9637781B2 (en) * 2012-02-14 2017-05-02 The Johns Hopkins University MiRNA analysis methods
CN102703584B (zh) * 2012-04-20 2013-08-21 中国人民解放军第四军医大学 自组装聚合分支dna探针的制备方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021271A1 (fr) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Analysetm d'elements genetiques induite par la ligase/polymerase de polymorphismes de mononucleotides et son utilisation dans des analyses genetiques
WO1999022030A1 (fr) * 1997-10-28 1999-05-06 The Regents Of The University Of California Identification du polymorphisme adn par la cytometrie de flux
EP1002877A2 (fr) * 1998-11-09 2000-05-24 Sanko Junyaku Co., Ltd. Procédé pour l'amplification génique
WO2001075157A1 (fr) * 2000-03-31 2001-10-11 Sanko Junyaku Co., Ltd. Sonde servant a fabriquer un polymere sonde, procede de fabrication d'un polymere sonde et son utilisation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU218095B (hu) * 1990-05-01 2000-05-28 Amgen Inc. Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban
AU6088094A (en) * 1993-01-15 1994-08-15 General Hospital Corporation, The Rna assays using rna binary probes and ribozyme ligase
US6277578B1 (en) * 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
JP3912595B2 (ja) * 2000-10-11 2007-05-09 三光純薬株式会社 プローブ自己集合体の作製方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021271A1 (fr) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Analysetm d'elements genetiques induite par la ligase/polymerase de polymorphismes de mononucleotides et son utilisation dans des analyses genetiques
WO1999022030A1 (fr) * 1997-10-28 1999-05-06 The Regents Of The University Of California Identification du polymorphisme adn par la cytometrie de flux
EP1002877A2 (fr) * 1998-11-09 2000-05-24 Sanko Junyaku Co., Ltd. Procédé pour l'amplification génique
WO2001075157A1 (fr) * 2000-03-31 2001-10-11 Sanko Junyaku Co., Ltd. Sonde servant a fabriquer un polymere sonde, procede de fabrication d'un polymere sonde et son utilisation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MORRIS T. ET AL.: "Rapid reserve transcription-PCR detection of hepatitis C virus RNA in serum by using the TaqMan fluorogenic detection system", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 34, no. 12, 1996, pages 2933 - 2936, XP001052728 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004072302A1 (fr) * 2003-02-14 2004-08-26 Eisai Co., Ltd. Procede d'amplification de signaux destine a la detection du gene exprime
US7632639B2 (en) 2003-02-21 2009-12-15 Eisai R&D Management Co., Ltd. Signal amplification method for detecting mutated gene
WO2007037282A1 (fr) * 2005-09-27 2007-04-05 Eisai R & D Management Co., Ltd. Procédé de formation d'un autoagrégat sur une microparticule et procédé de détection d'un analyte cible
JPWO2007037282A1 (ja) * 2005-09-27 2009-04-09 エーザイ・アール・アンド・ディー・マネジメント株式会社 微小粒子に自己集合体を形成させる方法及び標的分析物の検出方法
JP4847961B2 (ja) * 2005-09-27 2011-12-28 エーザイ・アール・アンド・ディー・マネジメント株式会社 微小粒子に自己集合体を形成させる方法及び標的分析物の検出方法
WO2007108378A1 (fr) 2006-03-15 2007-09-27 Eisai R & D Management Co., Ltd. Procédé de formation d'un polymère de sondes de signal
US7927804B2 (en) 2006-03-15 2011-04-19 Eisai & Managment Co., Ltd. Method of forming signal probe-polymer
EP2202319A4 (fr) * 2007-10-24 2010-10-20 Eisai R&D Man Co Ltd Sonde à acide nucléique et procédé de production d'un polymère pour sonde
JPWO2009054320A1 (ja) * 2007-10-24 2011-03-03 エーザイ・アール・アンド・ディー・マネジメント株式会社 核酸プローブ及びプローブポリマーの形成方法

Also Published As

Publication number Publication date
CN1388831A (zh) 2003-01-01
KR20020065471A (ko) 2002-08-13
AU7873001A (en) 2002-03-13
CA2389310A1 (fr) 2002-03-07
EP1229131A1 (fr) 2002-08-07
US20030175689A1 (en) 2003-09-18

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