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MX9704842A - Deecction de secuencias del acido nucleico, que emplean sondas de amplificacion. - Google Patents

Deecction de secuencias del acido nucleico, que emplean sondas de amplificacion.

Info

Publication number
MX9704842A
MX9704842A MX9704842A MX9704842A MX9704842A MX 9704842 A MX9704842 A MX 9704842A MX 9704842 A MX9704842 A MX 9704842A MX 9704842 A MX9704842 A MX 9704842A MX 9704842 A MX9704842 A MX 9704842A
Authority
MX
Mexico
Prior art keywords
nucleic acid
cross
probes
linking
side chain
Prior art date
Application number
MX9704842A
Other languages
English (en)
Inventor
David Albagli
Reuel Vanatta
Michael Wood
Original Assignee
Naxcor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/364,339 external-priority patent/US5616464A/en
Application filed by Naxcor filed Critical Naxcor
Publication of MX9704842A publication Critical patent/MX9704842A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1883—Means for temperature control using thermal insulation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyrrole Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Other In-Based Heterocyclic Compounds (AREA)
  • Hydrogenated Pyridines (AREA)
  • Plural Heterocyclic Compounds (AREA)

Abstract

Se dan a conocer métodos y composiciones para detectar secuencias de ácidos nucleicos. En particular, se emplean parejas de sondas, donde estas parejas definen una secuencia contigua substancialmente en el ácido nucleico objetivo. Cada una de las parejas tiene una cadena lateral, la cual forma un tronco de las dos cadenas laterales que se unen no covalentemente y son capaces de formar un entrelazamiento durante la activacion, donde las sondas y el ácido nucleico de muestra forman parejas de bases. El entrelazamiento de los troncos, cuando no se unen a los ADN complementarios, es inhibido. Cada uno de los ácidos nucleicos está presente inicialmente como un ácido nucleico de un solo cordon, para permitir el emparejamiento de bases, de modo que las sondas se unan al ácido nucleico objetivo homologo. La mezcla de ensayo se activa para suministrar el entrelazamiento, el ácido nucleico de doble cordon se funde, y el proceso del emparejamiento de bases, activacion y fusion se repite por un numero de ciclos suficiente para suministrar una cantidad detectable de sondas entrelazadas. Para inhibir el entrelazamiento de fondo, las cadenas laterales pueden suministrar una formacion doble, donde una porcion de la cadena lateral se une a una porcion diferente de la cadena lateral o la porcion de la sonda homologa al objetivo. También se suministran equipos que comprenden reactivos, al igual que dispositivos automáticos, para llevar a cabo el presente método.
MX9704842A 1994-12-27 1997-06-26 Deecction de secuencias del acido nucleico, que emplean sondas de amplificacion. MX9704842A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/364,339 US5616464A (en) 1994-12-27 1994-12-27 Nucleic acid sequence detection employing amplification probes
US08/487,034 US5767259A (en) 1994-12-27 1995-06-07 Oligonucleotides containing base-free linking groups with photoactivatable side chains

Publications (1)

Publication Number Publication Date
MX9704842A true MX9704842A (es) 1998-07-31

Family

ID=27002432

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9704842A MX9704842A (es) 1994-12-27 1997-06-26 Deecction de secuencias del acido nucleico, que emplean sondas de amplificacion.

Country Status (7)

Country Link
US (4) US5767259A (es)
EP (1) EP0796346A4 (es)
JP (1) JPH10512746A (es)
AU (1) AU718159B2 (es)
CA (1) CA2208794A1 (es)
MX (1) MX9704842A (es)
WO (1) WO1996020289A1 (es)

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US6004513A (en) 1999-12-21
US5767259A (en) 1998-06-16
US6177243B1 (en) 2001-01-23
US20030039961A1 (en) 2003-02-27
WO1996020289A1 (en) 1996-07-04
CA2208794A1 (en) 1996-07-04
US6590091B2 (en) 2003-07-08
JPH10512746A (ja) 1998-12-08
AU718159B2 (en) 2000-04-06
EP0796346A4 (en) 2004-06-23
AU4609396A (en) 1996-07-19

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