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WO2001035069A1 - Procede et dispositif permettant la preparation d'echantillons biologiques pour une analyse d'adn - Google Patents

Procede et dispositif permettant la preparation d'echantillons biologiques pour une analyse d'adn Download PDF

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Publication number
WO2001035069A1
WO2001035069A1 PCT/DE2000/003772 DE0003772W WO0135069A1 WO 2001035069 A1 WO2001035069 A1 WO 2001035069A1 DE 0003772 W DE0003772 W DE 0003772W WO 0135069 A1 WO0135069 A1 WO 0135069A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
measuring station
controller
quality
samples
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2000/003772
Other languages
German (de)
English (en)
Inventor
Arno Heuermann
Ivo Glynne Gut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biopsytec GmbH
Original Assignee
Biopsytec GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biopsytec GmbH filed Critical Biopsytec GmbH
Priority to AU19951/01A priority Critical patent/AU1995101A/en
Publication of WO2001035069A1 publication Critical patent/WO2001035069A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples

Definitions

  • the invention relates to a method and a device for processing biological samples for DNA analysis.
  • WO-A 93/259 12 discloses an analysis device which uses DNA to purify and isolate DNA by magnetic means Allows particles. A standardization and quality control of samples is not described here.
  • the printed documents EP-A 0 644 426, DE-A 37 33 098, DE-A 34 02 304, DE-C 33 46 532 and DE-A 37 36 632 describe devices for analyzing microbiological samples, all devices having in common that the samples are not subjected to quality control and are not standardized.
  • WO-A 97/22 882 discloses a production line which can be used for the treatment of biological and chemical samples and which is suitable for the mass analysis of DNA. The lack of quality control and standardization of the samples used makes it clear that this invention is not a complete production line. Can not yet be tegriert in ⁇ benvorkunq the lack of product m this GERAT today.
  • the prior art does not describe an automatic method for sample preparation in which the biological sample is subjected to quality control and, controlled by quantitative analysis methods, is brought to a standardized concentration by adding suitable solvents.
  • the object of the present invention is therefore to provide a method which overcomes the disadvantages of the prior art.
  • the object is achieved by an automatic method in which the biological sample is introduced into a device in a sample container, where it is transported, controlled by a bidirectional conveyor belt, to a quality measuring station by a controller.
  • the sample is UV-adsorbed or the like. examined and then sent to a quantification station.
  • the quantitative measurement it follows ⁇ means fluorine essence measurements, for example using mterkalierenden dyes.
  • the method according to the invention can be regarded as a supplementary module for mass DNA analysis.
  • the sample By connecting the controller of the device to an interface, the sample is fed to further analysis devices and / or laboratory robots. After setting the desired, standardized concentration and quality, the sample is taken from the device and processed further.
  • Another object of the present invention is an apparatus for performing the method according to the invention.
  • a device for processing biological samples for DNA analysis comprising a bidirectional conveying device 11 for sample holder 6, a quality measuring station 9, a quantity measuring station 10, at least one reagent container 3 with a downstream metering device 2 and a controller 4.
  • the bidirectional conveying device 11 is annular. It is particularly advantageous that sample inlet 7 and sample outlet 8 are arranged in the same position.
  • the quality measurement station 9 and / or the quantity measurement station 10 m further comprises a photometric, fluorometric, potentiometric, electrochemical, densitometric and / or refractometric measuring device.
  • the metering device 2 is an electrically or pneumatically controlled valve, a hose pump, a piston pump or a gear pump.
  • the controller 4 is a computer. It is particularly preferred according to the invention that the controller 4 is connected to an interface 5, the interface 5 being connected to further analysis devices and / or laboratory robots and receiving or forwarding measurement and control values from them.
  • the device according to the invention now combines the individual steps mentioned, which previously had to be carried out in succession as independent, independent devices and / or methods, to form a logistical unit, so that on the one hand the workload and thus the costs for these absolutely necessary steps be drastically reduced and, on the other hand, possible errors due to human error are excluded.
  • the device according to the invention for the preparation of biological samples for the subsequent DNA analysis is characterized in that due to the logical combination, realized by a controller or an external computer or the like.
  • biological samples in the laboratory sample containers, individual reaction vessels or microtiter plates or the like are examined and evaluated with regard to their quality by means of the UV absorption method or the like and their quantity by means of fluorescence measurement using tcaling dyes or the like and then by targeted and controlled addition of suitable solvents to one that can be selected by the operator or to one predefined concentration are diluted in another way in order to achieve a standardized sample format necessary for further processing.
  • the device according to the invention is furthermore characterized by a control of the internal or external control of the device via suitable interfaces by the machines which are used for the preceding and subsequent sample processing.
  • the device according to the invention is further characterized by the integration of further functions for processing samples against the background of DNA analysis.
  • characteristic data of the process steps carried out are additionally recorded in an internal or external database.
  • Figure 1 is a schematic arrangement of an inventive device in a first embodiment.
  • the sample containers 6, which already contain the samples to be examined, are fed into the device via the sample inlet 7.
  • the entire device is advantageously combined in a housing 1.
  • the sample container 6 is now introduced into the quality measuring station 9 and into the quantity measuring station 10 and is measured there in each case.
  • the sample can be inside of the device according to the invention can be moved to any location, for example to be measured there, or to be provided with chemicals and / or reagents and / or solvents, buffers and the like on the dosing devices 2, which are fed by reagent containers 3.
  • the number of reagent containers 3 is not restricted and is selected in such a way that the required additions of the media are ensured. If measurements have to follow the addition of the media, the samples are moved again to the measuring stations 9 and 10 by means of the bidirectional conveying device 11. If the sample has been conditioned in the respective sample container 6 in accordance with the specification, the corresponding sample container 6 is removed from the device at the sample outlet 8.
  • the parameters required for the subsequent analysis of the samples are determined externally and the controller 4 of the device is entered via the 5 m interface.
  • the interface 5 also forms the connection to the further automatic analyzers and / or laboratory robots (not shown here) for the manual handling of sample containers and the like.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé et un dispositif permettant la préparation d'échantillons biologiques pour une analyse d'ADN. Ce procédé consiste : a) à introduire l'échantillon dans un dispositif automatique, b) à mesurer la qualité de l'échantillon, c) à déterminer la quantité d'échantillon, d) à comparer la valeur de mesure à une valeur théorique, e) à modifier la concentration de l'échantillon, d'autant que la valeur de mesure s'écarte de la valeur théorique, f) à répéter les étapes c) à e) jusqu'à ce que ce que la valeur de mesure soit identique à la valeur théorique, puis g) à conduire l'échantillon normalisé à un traitement ultérieur. Le dispositif selon cette invention présente un degré d'automatisation élevé pour la préparation et/ou le conditionnement d'échantillons d'ADN.
PCT/DE2000/003772 1999-11-09 2000-10-18 Procede et dispositif permettant la preparation d'echantillons biologiques pour une analyse d'adn Ceased WO2001035069A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU19951/01A AU1995101A (en) 1999-11-09 2000-10-18 Method and device for preparing biological samples for dna analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19954840.4 1999-11-09
DE1999154840 DE19954840A1 (de) 1999-11-09 1999-11-09 Vorrichtung zur Aufbereitung biologischer Proben für die DNA-Analyse

Publications (1)

Publication Number Publication Date
WO2001035069A1 true WO2001035069A1 (fr) 2001-05-17

Family

ID=7929066

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/003772 Ceased WO2001035069A1 (fr) 1999-11-09 2000-10-18 Procede et dispositif permettant la preparation d'echantillons biologiques pour une analyse d'adn

Country Status (3)

Country Link
AU (1) AU1995101A (fr)
DE (1) DE19954840A1 (fr)
WO (1) WO2001035069A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005054923B3 (de) * 2005-11-17 2007-04-12 Siemens Ag Vorrichtung und Verfahren zur Aufbereitung einer Probe

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102010004467B4 (de) * 2010-01-13 2011-12-15 Polysius Ag Laborsystem und Verfahren zum Betreiben eines Laborsystems

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522493A (en) * 1981-11-19 1985-06-11 Olympus Optical Co., Ltd. Partial injection apparatus
JPS61110060A (ja) * 1984-11-02 1986-05-28 Hitachi Ltd 自動分析装置用自動再測定処理システム
JPS63271164A (ja) * 1987-04-28 1988-11-09 Shimadzu Corp 生化学自動分析システム
JPH04324363A (ja) * 1991-04-24 1992-11-13 Mitsui Toatsu Chem Inc 自動滴定装置
EP0703456A2 (fr) * 1994-09-21 1996-03-27 TOA MEDICAL ELECTRONICS CO., Ltd. Méthode et dispositif pour l'analyse automatique d'un échantillon
US5612473A (en) * 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
WO2000070087A1 (fr) * 1999-05-14 2000-11-23 Copernicus Therapeutics, Inc. Dispositif automatise de compactage d'acide nucleique

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3346532A1 (de) * 1982-12-22 1984-07-12 Olympus Optical Co., Ltd., Tokio/Tokyo Verfahren zum steuern eines analysiergeraets fuer chemische analysen
DE3402304C3 (de) * 1983-01-24 1995-11-09 Olympus Optical Co Verfahren für die automatische immunologische Analyse
EP0122772B1 (fr) * 1983-04-15 1988-08-17 Science and Technology Agency, Minister's Secretariat, Director of Finance Division Manipulateur chimique
JPH07119769B2 (ja) * 1986-10-01 1995-12-20 株式会社日立製作所 自動分析装置
DE3805808A1 (de) * 1988-02-24 1989-09-07 Europ Lab Molekularbiolog Automatische arbeitsstation fuer mikrobiologische arbeiten
US5595707A (en) * 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US5935522A (en) * 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
GB9212164D0 (en) * 1992-06-09 1992-07-22 Medical Res Council Preparation of nucleic acids
EP0644426B1 (fr) * 1993-09-17 2004-05-06 F. Hoffmann-La Roche Ag Analyseur comprenant un dispositif pour la suspension de particules, et méthode de suspension
GB9525794D0 (en) * 1995-12-18 1996-02-21 Hale Alan Biotechnological process

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4522493A (en) * 1981-11-19 1985-06-11 Olympus Optical Co., Ltd. Partial injection apparatus
JPS61110060A (ja) * 1984-11-02 1986-05-28 Hitachi Ltd 自動分析装置用自動再測定処理システム
JPS63271164A (ja) * 1987-04-28 1988-11-09 Shimadzu Corp 生化学自動分析システム
JPH04324363A (ja) * 1991-04-24 1992-11-13 Mitsui Toatsu Chem Inc 自動滴定装置
EP0703456A2 (fr) * 1994-09-21 1996-03-27 TOA MEDICAL ELECTRONICS CO., Ltd. Méthode et dispositif pour l'analyse automatique d'un échantillon
US5612473A (en) * 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
WO2000070087A1 (fr) * 1999-05-14 2000-11-23 Copernicus Therapeutics, Inc. Dispositif automatise de compactage d'acide nucleique

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 010, no. 290 (P - 503) 2 October 1986 (1986-10-02) *
PATENT ABSTRACTS OF JAPAN vol. 013, no. 087 (P - 835) 28 February 1989 (1989-02-28) *
PATENT ABSTRACTS OF JAPAN vol. 017, no. 161 (P - 1512) 29 March 1993 (1993-03-29) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005054923B3 (de) * 2005-11-17 2007-04-12 Siemens Ag Vorrichtung und Verfahren zur Aufbereitung einer Probe
US8551424B2 (en) 2005-11-17 2013-10-08 Siemens Aktiengesellschaft Apparatus for processing a sample comprising a biochip and reagents embedded in a biodegradable material, and processes thereof

Also Published As

Publication number Publication date
AU1995101A (en) 2001-06-06
DE19954840A1 (de) 2001-05-17

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