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WO2001088173A3 - Procedes d'assemblage enzymatique de polynucleotides et identification de polynucleotides possedant des caracteristiques souhaitees - Google Patents

Procedes d'assemblage enzymatique de polynucleotides et identification de polynucleotides possedant des caracteristiques souhaitees Download PDF

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Publication number
WO2001088173A3
WO2001088173A3 PCT/US2001/014966 US0114966W WO0188173A3 WO 2001088173 A3 WO2001088173 A3 WO 2001088173A3 US 0114966 W US0114966 W US 0114966W WO 0188173 A3 WO0188173 A3 WO 0188173A3
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Prior art keywords
polynucleotides
methods
identification
desired characteristics
present
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Ceased
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PCT/US2001/014966
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English (en)
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WO2001088173A2 (fr
Inventor
Simon Delagrave
Barry Marrs
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Hercules LLC
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Hercules LLC
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Publication date
Application filed by Hercules LLC filed Critical Hercules LLC
Priority to EP01933230A priority Critical patent/EP1282698A2/fr
Priority to AU2001259672A priority patent/AU2001259672A1/en
Publication of WO2001088173A2 publication Critical patent/WO2001088173A2/fr
Publication of WO2001088173A3 publication Critical patent/WO2001088173A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés servant à préparer deux grands polynucléotides de séquences arbitraires, de façon se prêtant sans difficulté à une automatisation. Elle concerne des procédés de préparation d'un polynucléotide possédant au moins 200 nucléotides soit dans un sens 5' à 3', soit 3' à 5', par ligation d'une pluralité d'oligonucléotides, dont l'assemblage représente la séquence de nucléotides du polynucléotide souhaité. Elle concerne également des banques de polynucléotides et le criblage de ces banques afin de rechercher des polynucléotides possédant des propriétés souhaitées.
PCT/US2001/014966 2000-05-16 2001-05-10 Procedes d'assemblage enzymatique de polynucleotides et identification de polynucleotides possedant des caracteristiques souhaitees Ceased WO2001088173A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01933230A EP1282698A2 (fr) 2000-05-16 2001-05-10 Procedes d'assemblage enzymatique de polynucleotides et identification de polynucleotides possedant des caracteristiques souhaitees
AU2001259672A AU2001259672A1 (en) 2000-05-16 2001-05-10 Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/571,774 2000-05-16
US09/571,774 US6479262B1 (en) 2000-05-16 2000-05-16 Solid phase enzymatic assembly of polynucleotides

Publications (2)

Publication Number Publication Date
WO2001088173A2 WO2001088173A2 (fr) 2001-11-22
WO2001088173A3 true WO2001088173A3 (fr) 2002-06-06

Family

ID=24284992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/014966 Ceased WO2001088173A2 (fr) 2000-05-16 2001-05-10 Procedes d'assemblage enzymatique de polynucleotides et identification de polynucleotides possedant des caracteristiques souhaitees

Country Status (5)

Country Link
US (2) US6479262B1 (fr)
EP (1) EP1282698A2 (fr)
AR (1) AR028454A1 (fr)
AU (1) AU2001259672A1 (fr)
WO (1) WO2001088173A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030049619A1 (en) * 2001-03-21 2003-03-13 Simon Delagrave Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides
EP1538980B1 (fr) * 2002-06-25 2017-01-18 Ultrashape Ltd. Dispositif pour l'esthetique corporelle
NL1022537C2 (nl) * 2003-01-31 2004-08-03 Univ Delft Tech Werkwijze voor de bereiding van chimere polynucleotiden.
WO2008027558A2 (fr) 2006-08-31 2008-03-06 Codon Devices, Inc. Assemblage itératif d'acides nucléiques utilisant l'activation de caractères codés par vecteurs
EP2285974A2 (fr) 2008-05-14 2011-02-23 British Columbia Cancer Agency Branch Synthèse de gènes par assemblage convergent de sous-ensembles d'oligonucléotides
WO2010025310A2 (fr) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Méthodes et dispositifs de synthèse de polynucléotides haute fidélité
US8999679B2 (en) 2008-12-18 2015-04-07 Iti Scotland Limited Method for assembly of polynucleic acid sequences
AP3396A (en) 2009-02-13 2015-08-31 Chem Inc X Methods of creating and screening DNA-encoded libraries
WO2011056872A2 (fr) 2009-11-03 2011-05-12 Gen9, Inc. Procédés et dispositifs microfluidiques pour la manipulation de gouttelettes dans un ensemble polynucléotidique haute fidélité
EP3597771A1 (fr) 2009-11-25 2020-01-22 Gen9, Inc. Procédés et appareils permettant la réduction des erreurs de l'adn basée sur une puce
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
EP2637780B1 (fr) * 2010-11-12 2022-02-09 Gen9, Inc. Puces à protéines et leurs procédés d'utilisation et de fabrication
EP2665833B1 (fr) 2011-01-17 2017-04-19 Life Technologies Corporation Workflow pour la détection de ligands à l'aide d'acides nucléiques
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
CN102876658A (zh) 2011-07-12 2013-01-16 北京唯尚立德生物科技有限公司 一种大规模合成长链核酸分子的方法
EP3954770A1 (fr) 2011-08-26 2022-02-16 Gen9, Inc. Compositions et procédés pour ensemble haute fidélité d'acides nucléiques
BR112014005205A2 (pt) * 2011-09-07 2017-03-21 X-Chem Inc métodos para etiquetar bibliotecas codificadas com dna
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013163263A2 (fr) 2012-04-24 2013-10-31 Gen9, Inc. Procédés de tri d'acides nucléiques et de clonage in vitro multiplex préparatoire
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
SG10201907347YA (en) 2012-07-13 2019-09-27 X Chem Inc Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
DK3164489T3 (da) * 2014-07-03 2020-08-10 Rhodx Inc Mærkning og vurdering af en målsekvens
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
WO2019140353A1 (fr) * 2018-01-12 2019-07-18 Camena Bioscience Limited Compositions et procédés de synthèse d'acides nucléiques enzymatique géométrique sans matrice
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
CN115109813A (zh) * 2022-02-09 2022-09-27 康龙化成(宁波)科技发展有限公司 一种一锅法实现5`-磷酸化以及寡聚核苷酸偶联的合成方法及应用

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983002626A1 (fr) * 1982-02-01 1983-08-04 Innovax Lab Ltd Procede de production de polyribonucleotides predetermines
GB2169605A (en) * 1984-12-26 1986-07-16 Nippon Shinyaku Co Ltd DNA synthesis
WO1990000626A1 (fr) * 1988-07-14 1990-01-25 Baylor College Of Medicine Assemblage en phase solide et reconstruction de biopolymeres
WO1993019202A2 (fr) * 1992-03-10 1993-09-30 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Reaction a matrice echangeable
WO1994014972A1 (fr) * 1992-12-23 1994-07-07 Edward David Hyman Procede et appareil de synthese enzymatique d'oligonucleotides
DE4343591A1 (de) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
DE19633427A1 (de) * 1996-08-20 1998-03-19 Hubert S Dr Bernauer Verfahren zur Synthese von Nukleinsäuremolekülen mit zumindest teilweise vorbestimmter Nukleotidsequenz
WO1998027230A1 (fr) * 1996-12-18 1998-06-25 Maxygen, Inc. Procedes et compositions pour l'ingenierie des polypeptides
WO1999014318A1 (fr) * 1997-09-16 1999-03-25 Board Of Regents, The University Of Texas System Synthese chimique complete et synthese de genes et de genomes
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
DE19812103A1 (de) * 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
DE19928591A1 (de) * 1998-06-22 1999-12-23 Roxana Havlina Herstellung von Polymeren aus nukleotidischen und/oder nicht-nukleotidischen Bausteinen auf enzymatischem Weg

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US6492107B1 (en) 1986-11-20 2002-12-10 Stuart Kauffman Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
DE3587814T2 (de) 1985-03-30 1994-11-10 Marc Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
US5262530A (en) 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
EP0406937A3 (en) 1989-06-30 1992-01-22 Eastman Kodak Company In vitro gene synthesis
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US5914245A (en) 1998-04-20 1999-06-22 Kairos Scientific Inc. Solid phase enzyme kinetics screening in microcolonies
AU4039400A (en) 1999-03-26 2000-10-16 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US20010053849A1 (en) 1999-06-16 2001-12-20 Mary Jeanne Kreek Plural biological sample arrays, and preparation and uses thereof

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1983002626A1 (fr) * 1982-02-01 1983-08-04 Innovax Lab Ltd Procede de production de polyribonucleotides predetermines
GB2169605A (en) * 1984-12-26 1986-07-16 Nippon Shinyaku Co Ltd DNA synthesis
WO1990000626A1 (fr) * 1988-07-14 1990-01-25 Baylor College Of Medicine Assemblage en phase solide et reconstruction de biopolymeres
WO1993019202A2 (fr) * 1992-03-10 1993-09-30 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Reaction a matrice echangeable
WO1994014972A1 (fr) * 1992-12-23 1994-07-07 Edward David Hyman Procede et appareil de synthese enzymatique d'oligonucleotides
DE4343591A1 (de) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
DE19633427A1 (de) * 1996-08-20 1998-03-19 Hubert S Dr Bernauer Verfahren zur Synthese von Nukleinsäuremolekülen mit zumindest teilweise vorbestimmter Nukleotidsequenz
WO1998027230A1 (fr) * 1996-12-18 1998-06-25 Maxygen, Inc. Procedes et compositions pour l'ingenierie des polypeptides
EP1149905A1 (fr) * 1996-12-18 2001-10-31 Maxygen, Inc. Procédés et compositions pour l'ingenierie des polypeptides
WO1999014318A1 (fr) * 1997-09-16 1999-03-25 Board Of Regents, The University Of Texas System Synthese chimique complete et synthese de genes et de genomes
DE19812103A1 (de) * 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
DE19928591A1 (de) * 1998-06-22 1999-12-23 Roxana Havlina Herstellung von Polymeren aus nukleotidischen und/oder nicht-nukleotidischen Bausteinen auf enzymatischem Weg
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BEATTIE K L ET AL: "SOLID-PHASE GENE ASSEMBLY", NATURE (LONDON), vol. 352, no. 6335, 1991, pages 548 - 549, XP002190391, ISSN: 0028-0836 *
KIKUCHI M ET AL: "An effective family shuffling method using single-stranded DNA", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 243, no. 1-2, February 2000 (2000-02-01), pages 133 - 137, XP004187682, ISSN: 0378-1119 *
NORD KARIN ET AL: "A combinatorial library of an alpha-helical bacterial receptor domain.", PROTEIN ENGINEERING, vol. 8, no. 6, 1995, pages 601 - 608, XP002190390, ISSN: 0269-2139 *
RAYNER S ET AL: "MerMade: An oligodeoxyribonucleotide synthesizer for high throughput oligonucleotide production in dual 96-well plates", PCR METHODS AND APPLICATIONS, COLD SPRING HARBOR, NY, US, vol. 8, no. 7, 1 July 1998 (1998-07-01), pages 741 - 747, XP002089330, ISSN: 1054-9803 *
See also references of EP1282698A2 *
VRATSKIKH ET AL: "Solid-phase synthesis of oligoribonucleotides using T4 RNA ligase and T4 polynucleotide kinase", BIOCHIMIE, PARIS, FR, vol. 77, no. 4, 1995, pages 227 - 232, XP002120689, ISSN: 0300-9084 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Also Published As

Publication number Publication date
AR028454A1 (es) 2003-05-07
WO2001088173A2 (fr) 2001-11-22
US6479262B1 (en) 2002-11-12
US6635453B2 (en) 2003-10-21
EP1282698A2 (fr) 2003-02-12
US20030082536A1 (en) 2003-05-01
AU2001259672A1 (en) 2001-11-26

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