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WO2001081619A3 - Pcr avec mesure de conductivite - Google Patents

Pcr avec mesure de conductivite Download PDF

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Publication number
WO2001081619A3
WO2001081619A3 PCT/DE2001/001025 DE0101025W WO0181619A3 WO 2001081619 A3 WO2001081619 A3 WO 2001081619A3 DE 0101025 W DE0101025 W DE 0101025W WO 0181619 A3 WO0181619 A3 WO 0181619A3
Authority
WO
WIPO (PCT)
Prior art keywords
pcr
conductivity
dna
during
quantification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2001/001025
Other languages
German (de)
English (en)
Other versions
WO2001081619A8 (fr
WO2001081619A2 (fr
Inventor
Borros Arneth
Alfons Arneth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE20007376U external-priority patent/DE20007376U1/de
Priority claimed from DE20007378U external-priority patent/DE20007378U1/de
Priority claimed from DE20013567U external-priority patent/DE20013567U1/de
Priority claimed from DE20018005U external-priority patent/DE20018005U1/de
Application filed by Individual filed Critical Individual
Priority to AU56115/01A priority Critical patent/AU5611501A/en
Publication of WO2001081619A2 publication Critical patent/WO2001081619A2/fr
Publication of WO2001081619A3 publication Critical patent/WO2001081619A3/fr
Publication of WO2001081619A8 publication Critical patent/WO2001081619A8/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
    • G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

L'activité de gènes individuels constitue une question importante en biologie moléculaire. Pour déterminer l'activité de gènes individuels, on effectue fréquemment une PCR (réaction en chaîne de la polymérase) ou une RT-PCR (PCR inverse). La complexité réside dans le fait que la PCR est difficilement quantifiable. Le principe de l'invention consiste à utiliser la variation de la conductivité à mesurer pendant le déroulement de la PCR, en tant que marqueur pour déterminer la progression de la PCR et quantifier la quantité d'ADN initiale. L'appareil selon l'invention détermine les variations de conductivité « en ligne » pendant le déroulement de la PCR, au moyen de petites micro-électrodes plongées dans la solution de PCR. Sur la base de ce paramètre, cet appareil est en mesure de calculer la quantité d'ADN ou d'ARN avant le déroulement de la réaction.
PCT/DE2001/001025 2000-04-22 2001-03-17 Pcr avec mesure de conductivite Ceased WO2001081619A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU56115/01A AU5611501A (en) 2000-04-22 2001-03-17 Conductivity pcr

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
DE20007376.1 2000-04-22
DE20007376U DE20007376U1 (de) 2000-04-22 2000-04-22 PCR-Leitfähigkeits-Mikroarrays
DE20007378U DE20007378U1 (de) 2000-04-22 2000-04-22 Phosphat-Leitfähigkeits-PCR
DE20007378.8 2000-04-22
US20419200P 2000-05-08 2000-05-08
US60/204,192 2000-05-08
US21942200P 2000-07-20 2000-07-20
US21942100P 2000-07-20 2000-07-20
US60/219422 2000-07-20
US60/219,421 2000-07-20
DE20013567.8 2000-08-08
DE20013567U DE20013567U1 (de) 2000-08-08 2000-08-08 Leitfähigkeits-PCR
DE20018005.3 2000-10-22
DE20018005U DE20018005U1 (de) 2000-10-22 2000-10-22 Sandwich-PCR-DNA-Microarray
DE10060256 2000-12-04
DE10060256.8 2000-12-04

Publications (3)

Publication Number Publication Date
WO2001081619A2 WO2001081619A2 (fr) 2001-11-01
WO2001081619A3 true WO2001081619A3 (fr) 2002-05-16
WO2001081619A8 WO2001081619A8 (fr) 2002-06-13

Family

ID=27570759

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/001025 Ceased WO2001081619A2 (fr) 2000-04-22 2001-03-17 Pcr avec mesure de conductivite

Country Status (1)

Country Link
WO (1) WO2001081619A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100858080B1 (ko) * 2002-11-12 2008-09-10 삼성전자주식회사 전기적 신호를 측정하는 ｐｃｒ 증폭 산물을 검출하는 방법
FR2941876B1 (fr) * 2009-02-06 2012-12-07 Bio Rad Pasteur Appareil de validation thermique, ensemble d'un dispositif de traitement d'echantillons biologiques et d'un tel appareil, et procede de fabrication d'un tel appareil.
WO2013150454A2 (fr) * 2012-04-04 2013-10-10 Shama Bhat Système et procédé de détection et d'analyse d'acide désoxyribonucléique amplifié
DE102013010961A1 (de) 2013-07-01 2015-01-08 Borros Arneth DNA Sequenzierung mittels Leitfähigkeitsmessung der DNA Polymerase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0488769A2 (fr) * 1990-11-29 1992-06-03 The Perkin-Elmer Corporation Dispositif à cycles thermiques pour l'exécution automatique de réactions en chaine de polymérase à régulation précise de température
WO1999010530A1 (fr) * 1997-08-22 1999-03-04 Molecular Sensors Limited Evaluation d'un acide nucleique
US5891639A (en) * 1993-11-12 1999-04-06 Geron Corporation Telomerase activity assays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0488769A2 (fr) * 1990-11-29 1992-06-03 The Perkin-Elmer Corporation Dispositif à cycles thermiques pour l'exécution automatique de réactions en chaine de polymérase à régulation précise de température
US5891639A (en) * 1993-11-12 1999-04-06 Geron Corporation Telomerase activity assays
WO1999010530A1 (fr) * 1997-08-22 1999-03-04 Molecular Sensors Limited Evaluation d'un acide nucleique

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J. P. SCHONFIELD: "a rapid semi-automated microtiter plate method for analysis and sequencing by PCR from bacterial stocks", NUCLEIC ACIDS RESEARCH, vol. 17, no. 22, 1989, pages 9498, XP001053732 *

Also Published As

Publication number Publication date
WO2001081619A8 (fr) 2002-06-13
WO2001081619A2 (fr) 2001-11-01

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