DE60123481D1 - Verfahren zur durchführung einer subtraktiven hybridisierung - Google Patents

Verfahren zur durchführung einer subtraktiven hybridisierung

Info

Publication number: DE60123481D1
Authority: DE; Germany
Prior art keywords: carrying; subtractive hybridization; out subtractive; relates; oligonucleotides
Prior art date: 2000-08-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

DE60123481T

Other languages

English (en)

Other versions

DE60123481T2 (de

Inventor

G Birkenmeyer

P Leary

Scott Muerhoff

M Desai

K Mushahwar

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Abbott Laboratories

Original Assignee

Abbott Laboratories

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-08-02

Filing date

2001-08-02

Publication date

2006-11-09

2001-08-02 Application filed by Abbott Laboratories filed Critical Abbott Laboratories

2006-11-09 Publication of DE60123481D1 publication Critical patent/DE60123481D1/de

2007-06-21 Application granted granted Critical

2007-06-21 Publication of DE60123481T2 publication Critical patent/DE60123481T2/de

2021-08-03 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

238000000034 method Methods 0.000 title abstract 3
238000009396 hybridization Methods 0.000 title abstract 2
108091034117 Oligonucleotide Proteins 0.000 abstract 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
239000003153 chemical reaction reagent Substances 0.000 abstract 1
238000002955 isolation Methods 0.000 abstract 1
238000012986 modification Methods 0.000 abstract 1
230000004048 modification Effects 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Biotechnology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Led Device Packages (AREA)
Saccharide Compounds (AREA)

DE60123481T 2000-08-02 2001-08-02 Verfahren zur durchführung einer subtraktiven hybridisierung Expired - Lifetime DE60123481T2 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US09/631,349 US6455255B1 (en)	2000-08-02	2000-08-02	Method of performing subtractive hybridization using RDA
US631349		2000-08-02
PCT/US2001/024480 WO2002010458A2 (en)	2000-08-02	2001-08-02	Method of performing subtractive hybridization

Publications (2)

Publication Number	Publication Date
DE60123481D1 true DE60123481D1 (de)	2006-11-09
DE60123481T2 DE60123481T2 (de)	2007-06-21

Family

ID=24530821

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE60123481T Expired - Lifetime DE60123481T2 (de)	2000-08-02	2001-08-02	Verfahren zur durchführung einer subtraktiven hybridisierung

Country Status (8)

Country	Link
US (1)	US6455255B1 (de)
EP (1)	EP1364048B1 (de)
JP (1)	JP4856842B2 (de)
AT (1)	ATE340873T1 (de)
CA (1)	CA2417501A1 (de)
DE (1)	DE60123481T2 (de)
ES (1)	ES2272516T3 (de)
WO (1)	WO2002010458A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN1264744A (zh) *	1999-02-26	2000-08-30	上海生元基因开发有限公司	一种通过循环差减进行大规模cDNA克隆和测序的方法
KR100870222B1 (ko) *	2000-12-19	2008-11-24	산또리 가부시키가이샤	유전자 농축 방법
US6632611B2 (en) *	2001-07-20	2003-10-14	Affymetrix, Inc.	Method of target enrichment and amplification
GB2412170A (en) *	2004-03-16	2005-09-21	Plant Bioscience Ltd	A method for enriching for target nucleic acids in a sample
WO2006093082A1 (ja) *	2005-03-01	2006-09-08	Wako Pure Chemical Industries, Ltd.	サブトラクション・ポリヌクレオチドの取得方法
WO2007100384A2 (en) *	2005-10-24	2007-09-07	Invitrogen Corporation	Three frame cdna expression libraries for functional screening of proteins
US9388457B2 (en)	2007-09-14	2016-07-12	Affymetrix, Inc.	Locus specific amplification using array probes
WO2011030154A1 (en) *	2009-09-12	2011-03-17	Zoragen Biotechnologies Llp	Detection of quantitative genetic differences

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4683195A (en)	1986-01-30	1987-07-28	Cetus Corporation	Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5436142A (en) *	1992-11-12	1995-07-25	Cold Spring Harbor Laboratory	Methods for producing probes capable of distingushing variant genomic sequences
US5958738A (en)	1997-03-24	1999-09-28	Roche Diagnostics Corporation	Procedure for subtractive hybridization and difference analysis
CA2307674C (en) *	1997-10-30	2013-02-05	Cold Spring Harbor Laboratory	Probe arrays and methods of using probe arrays for distinguishing dna
DE69932418D1 (de) *	1998-03-18	2006-08-31	Quark Biotech Inc	Selektion/subtraktionsansatz zur genidentifizierung
EP1127163A2 (de) *	1998-11-02	2001-08-29	The Brigham & Women's Hospital, Inc.	Eine methode zur genomischen subtraktiven hybridisierung
JP2000139461A (ja) *	1998-11-06	2000-05-23	Hitachi Koki Co Ltd	核酸サブトラクション法及びその装置
CN1264744A (zh) *	1999-02-26	2000-08-30	上海生元基因开发有限公司	一种通过循环差减进行大规模cDNA克隆和测序的方法
JP2000308489A (ja) *	1999-04-28	2000-11-07	Dai Ichi Seiyaku Co Ltd	微小変異ｄｎａ検出方法

2000
- 2000-08-02 US US09/631,349 patent/US6455255B1/en not_active Expired - Fee Related
2001
- 2001-08-02 JP JP2002516374A patent/JP4856842B2/ja not_active Expired - Fee Related
- 2001-08-02 ES ES01957439T patent/ES2272516T3/es not_active Expired - Lifetime
- 2001-08-02 CA CA002417501A patent/CA2417501A1/en not_active Abandoned
- 2001-08-02 WO PCT/US2001/024480 patent/WO2002010458A2/en not_active Ceased
- 2001-08-02 DE DE60123481T patent/DE60123481T2/de not_active Expired - Lifetime
- 2001-08-02 AT AT01957439T patent/ATE340873T1/de not_active IP Right Cessation
- 2001-08-02 EP EP01957439A patent/EP1364048B1/de not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DE60123481T2 (de)	2007-06-21
CA2417501A1 (en)	2002-02-07
ATE340873T1 (de)	2006-10-15
ES2272516T3 (es)	2007-05-01
WO2002010458A8 (en)	2007-07-05
WO2002010458A2 (en)	2002-02-07
US6455255B1 (en)	2002-09-24
JP2004516014A (ja)	2004-06-03
EP1364048A2 (de)	2003-11-26
EP1364048B1 (de)	2006-09-27
JP4856842B2 (ja)	2012-01-18
WO2002010458A3 (en)	2003-09-04

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Legal Events

Date	Code	Title	Description
2007-10-18	8364	No opposition during term of opposition