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WO2001073114A3 - Reagents and methods for detecting a reduced cofactor - Google Patents

Reagents and methods for detecting a reduced cofactor Download PDF

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Publication number
WO2001073114A3
WO2001073114A3 PCT/US2001/007619 US0107619W WO0173114A3 WO 2001073114 A3 WO2001073114 A3 WO 2001073114A3 US 0107619 W US0107619 W US 0107619W WO 0173114 A3 WO0173114 A3 WO 0173114A3
Authority
WO
WIPO (PCT)
Prior art keywords
systems
analyte
sample
kits
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/007619
Other languages
French (fr)
Other versions
WO2001073114A2 (en
Inventor
Tianmei Ouyang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LifeScan Inc
Original Assignee
LifeScan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LifeScan Inc filed Critical LifeScan Inc
Priority to IL15126201A priority Critical patent/IL151262A0/en
Priority to EP01916527A priority patent/EP1268849A2/en
Priority to PL01357180A priority patent/PL357180A1/en
Priority to HK03102161.6A priority patent/HK1050030A1/en
Priority to KR1020027012698A priority patent/KR20020089412A/en
Priority to AU2001243542A priority patent/AU2001243542A1/en
Priority to JP2001570828A priority patent/JP2003528623A/en
Priority to CA002404421A priority patent/CA2404421A1/en
Priority to MXPA02009469A priority patent/MXPA02009469A/en
Publication of WO2001073114A2 publication Critical patent/WO2001073114A2/en
Publication of WO2001073114A3 publication Critical patent/WO2001073114A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90212—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Signal producing systems, reagent compositions, test strips and kits of the same, as well as methods for their use in the detection of an analyte in a sample, are provided. The subject signal producing systems are characterized by having at least a first and second electron transfer agent and a redox indicator, where in many preferred embodiments the systems include a proteinaceous and non-proteinaceous electron transfer agent, e.g. a phenazine compound and a diaphorase. In many preferred embodiments, the subject systems and kits further include at least one of and often both of an enzyme cofactor and an enzyme having an analyte oxidizing activity, e.g. an analyte dehydrogenase. The subject systems, reagent compositions, test strips and kits find use in the detection of a wide variety of analytes in a sample, such as a physiological sample, e.g. blood or a fraction thereof.
PCT/US2001/007619 2000-03-28 2001-03-08 Reagents and methods for detecting a reduced cofactor Ceased WO2001073114A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
IL15126201A IL151262A0 (en) 2000-03-28 2001-03-08 Reagents and methods for detecting a reduced cofactor
EP01916527A EP1268849A2 (en) 2000-03-28 2001-03-08 Reagents and methods for detecting a reduced cofactor
PL01357180A PL357180A1 (en) 2000-03-28 2001-03-08 Reagent systems for detecting the presence of a reduced cofactor in a sample and methods for using the same
HK03102161.6A HK1050030A1 (en) 2000-03-28 2001-03-08 Reagents and methods for detecting a reduced cofactor
KR1020027012698A KR20020089412A (en) 2000-03-28 2001-03-08 Reagents and methods for detecting a reduced cofactor
AU2001243542A AU2001243542A1 (en) 2000-03-28 2001-03-08 Reagent systems for detecting the presence of a reduced cofactor in a sample and methods for using the same
JP2001570828A JP2003528623A (en) 2000-03-28 2001-03-08 Reagent system for detecting the presence of reduced cofactor in a sample and methods of use thereof
CA002404421A CA2404421A1 (en) 2000-03-28 2001-03-08 Reagent systems for detecting the presence of a reduced cofactor in a sample and methods for using the same
MXPA02009469A MXPA02009469A (en) 2000-03-28 2001-03-08 Reagent systems for detecting the presence of a reduced cofactor in a sample and methods for using the same.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53720300A 2000-03-28 2000-03-28
US09/537,203 2000-03-28

Publications (2)

Publication Number Publication Date
WO2001073114A2 WO2001073114A2 (en) 2001-10-04
WO2001073114A3 true WO2001073114A3 (en) 2002-03-28

Family

ID=24141653

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/007619 Ceased WO2001073114A2 (en) 2000-03-28 2001-03-08 Reagents and methods for detecting a reduced cofactor

Country Status (13)

Country Link
EP (1) EP1268849A2 (en)
JP (1) JP2003528623A (en)
KR (1) KR20020089412A (en)
CN (1) CN1419604A (en)
AR (1) AR027726A1 (en)
AU (1) AU2001243542A1 (en)
CA (1) CA2404421A1 (en)
HK (1) HK1050030A1 (en)
IL (1) IL151262A0 (en)
MX (1) MXPA02009469A (en)
PL (1) PL357180A1 (en)
RU (1) RU2266543C2 (en)
WO (1) WO2001073114A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7452457B2 (en) 2003-06-20 2008-11-18 Roche Diagnostics Operations, Inc. System and method for analyte measurement using dose sufficiency electrodes
US7556723B2 (en) 2004-06-18 2009-07-07 Roche Diagnostics Operations, Inc. Electrode design for biosensor
US7569126B2 (en) 2004-06-18 2009-08-04 Roche Diagnostics Operations, Inc. System and method for quality assurance of a biosensor test strip
US7597793B2 (en) 2003-06-20 2009-10-06 Roche Operations Ltd. System and method for analyte measurement employing maximum dosing time delay
US7604721B2 (en) 2003-06-20 2009-10-20 Roche Diagnostics Operations, Inc. System and method for coding information on a biosensor test strip

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050103624A1 (en) 1999-10-04 2005-05-19 Bhullar Raghbir S. Biosensor and method of making
GB0204232D0 (en) * 2002-02-22 2002-04-10 Isis Innovation Assay
US7749437B2 (en) 2003-06-20 2010-07-06 Roche Diagnostics Operations, Inc. Method and reagent for producing narrow, homogenous reagent stripes
US8148164B2 (en) 2003-06-20 2012-04-03 Roche Diagnostics Operations, Inc. System and method for determining the concentration of an analyte in a sample fluid
US8071030B2 (en) 2003-06-20 2011-12-06 Roche Diagnostics Operations, Inc. Test strip with flared sample receiving chamber
ITMI20050881A1 (en) * 2005-05-16 2006-11-17 Anna Favre DIAGNOSTIC KIT FOR HIRSCHSPRUNG MEGALON CONGENITAL DISEASE
DE102006002165A1 (en) * 2006-01-17 2007-07-19 Merck Patent Gmbh Determining acetate content in a sample, comprises contacting a sample with test strip comprising e.g. tetrazolium salt, incubating the test strip, contacting test strip with phenazine methosulfate reagent and observing color change
CN102884429B (en) * 2010-03-01 2014-11-19 系统生物股份有限公司 Test strip for the detection of equol
CN102520198A (en) * 2011-11-21 2012-06-27 宁波美康生物科技股份有限公司 Ethanol concentration detection kit and manufacture method thereof
CN102520150A (en) * 2011-12-08 2012-06-27 上海高丰医疗电器有限公司 Diagnostic test paper for determination of gamma-hydroxybutanoic acid and preparation method thereof
US8921061B2 (en) 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Reagent materials and associated test elements
US8920628B2 (en) 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Systems and methods for multiple analyte analysis
CN108359710B (en) * 2018-02-11 2019-04-30 山东大学齐鲁医院 Enzyme chromogenic quantitative assay kit and assay method for glucose phosphate isomerase
CN110117791B (en) * 2019-05-23 2023-11-10 电子科技大学 Binding force promoter for brown oxide liquid of high-density interconnection printed circuit board

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4629697A (en) * 1982-12-24 1986-12-16 Merck Patent Gesellschaft Mit Beschrankter Haftung Test system and procedure for the determination of NAD (P) H
EP0279988A1 (en) * 1986-12-16 1988-08-31 Enzymatics, Inc. Digital threshold colour control system
US4824779A (en) * 1984-02-29 1989-04-25 Kyowa Medex Co., Ltd. Method for the determination of the reduced form of nicotinamide adenine dinucleotide
US5278047A (en) * 1989-04-25 1994-01-11 Lilja Jan E Method of analysis, reagent composition and use thereof for glucose determination
US5902731A (en) * 1998-09-28 1999-05-11 Lifescan, Inc. Diagnostics based on tetrazolium compounds

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4311464A1 (en) * 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Method for the colorimetric determination of an analyte with a PQQ-dependent dehydrogenase

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4629697A (en) * 1982-12-24 1986-12-16 Merck Patent Gesellschaft Mit Beschrankter Haftung Test system and procedure for the determination of NAD (P) H
US4824779A (en) * 1984-02-29 1989-04-25 Kyowa Medex Co., Ltd. Method for the determination of the reduced form of nicotinamide adenine dinucleotide
EP0279988A1 (en) * 1986-12-16 1988-08-31 Enzymatics, Inc. Digital threshold colour control system
US5278047A (en) * 1989-04-25 1994-01-11 Lilja Jan E Method of analysis, reagent composition and use thereof for glucose determination
US5902731A (en) * 1998-09-28 1999-05-11 Lifescan, Inc. Diagnostics based on tetrazolium compounds

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RAAP A K ET AL: "THE PHENAZINE METHOSULFATE TETRAZOLIUM CAPTURE REACTION IN NADP DEPENDENT DEHYDROGENASE CYTOCHEMISTRY 2. A NOVEL HYPOTHESIS FOR THE MODE OF ACTION OF PHENAZINE METHOSULFATE AND A STUDY OF THE PROPERTIES OF REDUCED PHENAZINE METHOSULFATE", HISTOCHEMICAL JOURNAL, vol. 15, no. 9, 1983, pages 881 - 894, XP001050450, ISSN: 0018-2214 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7452457B2 (en) 2003-06-20 2008-11-18 Roche Diagnostics Operations, Inc. System and method for analyte measurement using dose sufficiency electrodes
US7597793B2 (en) 2003-06-20 2009-10-06 Roche Operations Ltd. System and method for analyte measurement employing maximum dosing time delay
US7604721B2 (en) 2003-06-20 2009-10-20 Roche Diagnostics Operations, Inc. System and method for coding information on a biosensor test strip
US7556723B2 (en) 2004-06-18 2009-07-07 Roche Diagnostics Operations, Inc. Electrode design for biosensor
US7569126B2 (en) 2004-06-18 2009-08-04 Roche Diagnostics Operations, Inc. System and method for quality assurance of a biosensor test strip

Also Published As

Publication number Publication date
AU2001243542A1 (en) 2001-10-08
WO2001073114A2 (en) 2001-10-04
CN1419604A (en) 2003-05-21
RU2266543C2 (en) 2005-12-20
MXPA02009469A (en) 2003-02-24
KR20020089412A (en) 2002-11-29
CA2404421A1 (en) 2001-10-04
RU2002123839A (en) 2004-03-10
AR027726A1 (en) 2003-04-09
PL357180A1 (en) 2004-07-26
IL151262A0 (en) 2003-04-10
EP1268849A2 (en) 2003-01-02
HK1050030A1 (en) 2003-06-06
JP2003528623A (en) 2003-09-30

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