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WO1998031840B1 - Procedes destines a l'isolement de matieres biologiques cibles au moyen de particules magnetiques de silice - Google Patents

Procedes destines a l'isolement de matieres biologiques cibles au moyen de particules magnetiques de silice

Info

Publication number
WO1998031840B1
WO1998031840B1 PCT/US1998/001149 US9801149W WO9831840B1 WO 1998031840 B1 WO1998031840 B1 WO 1998031840B1 US 9801149 W US9801149 W US 9801149W WO 9831840 B1 WO9831840 B1 WO 9831840B1
Authority
WO
WIPO (PCT)
Prior art keywords
biological target
target material
isolating
silica magnetic
complex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/001149
Other languages
English (en)
Other versions
WO1998031840A1 (fr
Filing date
Publication date
Priority claimed from US08/785,097 external-priority patent/US6027945A/en
Application filed filed Critical
Priority to AU66475/98A priority Critical patent/AU732756B2/en
Priority to DE0895546T priority patent/DE895546T1/de
Priority to EP98908435A priority patent/EP0895546B1/fr
Priority to JP53467498A priority patent/JP3253638B2/ja
Priority to DE69817484T priority patent/DE69817484T2/de
Publication of WO1998031840A1 publication Critical patent/WO1998031840A1/fr
Publication of WO1998031840B1 publication Critical patent/WO1998031840B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

La présente invention concerne des procédés destinés à l'isolement de matières biologiques cibles, en particulier d'acides nucléiques tels que de l'ADN, de l'ARN ou des molécules hybrides d'ADN et d'ARN, d'autres substances présentes dans un milieu à l'aide de particules magnétiques de silice. Les procédés de la présente invention comportent les étapes consistant à former un complexe constitué de particules magnétiques de silice et de matière biologique cible par préparation d'un mélange contenant le milieu et des particules; séparer le complexe du mélange au moyen d'une force magnétique extérieure, et éluer la matière biologique cible du complexe. Dans les modes de réalisation préférés, les particules magnétiques de silice utilisées dans les procédés et équipements de la présente invention sont susceptibles de former un complexe avec au moins 2 νg de matière biologique cible par milligramme de particules, et de dégager au moins 60 % de la matière contenue dans le complexe au cours de l'étape d'élution du procédé. Les procédés de la présente invention permettent de produire de la matière biologique cible isolée qui est sensiblement exempte de contaminants, tels des métaux ou des substances macromoléculaires, susceptibles de provoquer par leur présence des interférences au cours de traitements ou d'analyses ultérieures.
PCT/US1998/001149 1997-01-21 1998-01-21 Procedes destines a l'isolement de matieres biologiques cibles au moyen de particules magnetiques de silice Ceased WO1998031840A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU66475/98A AU732756B2 (en) 1997-01-21 1998-01-21 Methods of isolating biological target materials using silica magnetic particles
DE0895546T DE895546T1 (de) 1997-01-21 1998-01-21 Verfahren zur isolierung bestimmter biologischer stoffe mittels magnetischer silika-partikel
EP98908435A EP0895546B1 (fr) 1997-01-21 1998-01-21 Procedes destines a l'isolement de matieres biologiques cibles au moyen de particules magnetiques de silice
JP53467498A JP3253638B2 (ja) 1997-01-21 1998-01-21 シリカ磁気粒子を使用する生物学的目標物質の分離法
DE69817484T DE69817484T2 (de) 1997-01-21 1998-01-21 Verfahren zur isolierung bestimmter biologischer stoffe mittels magnetischer silika-partikel

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/785,097 1997-01-21
US08/785,097 US6027945A (en) 1997-01-21 1997-01-21 Methods of isolating biological target materials using silica magnetic particles

Publications (2)

Publication Number Publication Date
WO1998031840A1 WO1998031840A1 (fr) 1998-07-23
WO1998031840B1 true WO1998031840B1 (fr) 1998-09-17

Family

ID=25134438

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/001149 Ceased WO1998031840A1 (fr) 1997-01-21 1998-01-21 Procedes destines a l'isolement de matieres biologiques cibles au moyen de particules magnetiques de silice

Country Status (9)

Country Link
US (5) US6027945A (fr)
EP (2) EP0895546B1 (fr)
JP (1) JP3253638B2 (fr)
AU (1) AU732756B2 (fr)
BR (1) BR9805897A (fr)
CA (1) CA2249393A1 (fr)
DE (3) DE895546T1 (fr)
DK (1) DK1367137T3 (fr)
WO (1) WO1998031840A1 (fr)

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US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor

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