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WO1998031840B1 - Methods of isolating biological target materials using silica magnetic particles - Google Patents

Methods of isolating biological target materials using silica magnetic particles

Info

Publication number
WO1998031840B1
WO1998031840B1 PCT/US1998/001149 US9801149W WO9831840B1 WO 1998031840 B1 WO1998031840 B1 WO 1998031840B1 US 9801149 W US9801149 W US 9801149W WO 9831840 B1 WO9831840 B1 WO 9831840B1
Authority
WO
WIPO (PCT)
Prior art keywords
biological target
target material
isolating
silica magnetic
complex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/001149
Other languages
French (fr)
Other versions
WO1998031840A1 (en
Filing date
Publication date
Priority claimed from US08/785,097 external-priority patent/US6027945A/en
Application filed filed Critical
Priority to DE0895546T priority Critical patent/DE895546T1/en
Priority to AU66475/98A priority patent/AU732756B2/en
Priority to JP53467498A priority patent/JP3253638B2/en
Priority to EP98908435A priority patent/EP0895546B1/en
Priority to DE69817484T priority patent/DE69817484T2/en
Publication of WO1998031840A1 publication Critical patent/WO1998031840A1/en
Publication of WO1998031840B1 publication Critical patent/WO1998031840B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

The present invention provides methods for isolating biological target materials, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from other substances in a medium using silica magnetic particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the biological target material in a mixture of the medium and particles, separating the complex from the mixture using external magnetic force, and eluting the biological target material from the complex. The preferred embodiments of magnetic silica particles used in the methods and kits of the present invention are capable of forming a complex with at least 2 νg of biological target material per milligram of particle, and of releasing at least 60 % of the material from the complex in the elution step of the method. The methods of the present invention produce isolated biological target material which is substantially free of contaminants, such as metals or macromolecular substances, which can interfere with further processing or analysis, if present.

Claims

AMENDED CLAIMS[received by the International Bureau on 21 July 1998 (21.07.98); original claims 1 , 5 and 6 amended; remaining claims unchanged (2 pages)]
1. A method for isolating a biological target material from other material in a medium by: a) providing a medium including the biological target material; b) providing silica magnetic particles capable of reversibly binding the biological target material; c) forming a complex of the silica magnetic particles and the biological target material by combining the silica magnetic particles and the medium; d) removing the complex from the medium by application of an external magnetic field; and e) separating the biological target material from the complex by eluting the biological target material, whereby the isolated biological target material is obtained.
2. A method of isolating a biological target material according to claim 1, wherein the biological material isolated according to the method consists of a nucleic acid.
3. A method of isolating a biological target material according to claim 1, wherein the silica magnetic particles provided in step (b) are capable of reversibly binding at least 2 micrograms of biological target material per milligram of particle.
4. A method of isolating a biological target material according to claim 3, wherein the silica magnetic particles provided in step (b) of the method are siliceous- oxide coated magnetic particles.
5. A method of isolating a biological target material according to claim 1, wherein at least 60% of the biological target material in the complex is eluted from the particles in step (e).
6. A method of isolating a biological target material according to claim 1, wherein the biological target material eluted from the complex in step (e) contains no more than 50 parts per million of transition metal contaminants.
7. A method of isolating a biological target material from other materials in a medium comprising the steps of: a) providing a medium containing the biological target material; b) providing a silica magnetic particle with the capacity to reversibly bind at least 2 micrograms of biological target material per milligram of particle; c) forming a mixture comprising the medium and the silica magnetic particle; d) adhering the biological target material to the silica magnetic particle in the mixture; e) removing the silica magnetic particle with the biological target material adhered thereto from the mixture by application of an external magnetic field; and f) eluting at least 60% of the biological target material from the silica magnetic particle by exposing the particle to an elution solution.
8. A method of isolating a biological target material according to claim 7, wherein the biological material isolated according to the method consists of a nucleic acid material.
9. A method of isolating a biological target material according to claim 8, wherein the nucleic acid biological target material isolated according to the method consists of a plasmid DNA material.
10. A method of isolating a biological target material according to claim 8, wherein the nucleic acid biological target material isolated consists of DNA fragment material.
39
STATEMENT UNDER ARTICLE 19
Applicant respectfully submits that none of the amendments to any of the claims presented herein adds any new matter to the subject international application. The amendments are introduced herein to clarify the subject matter of each claim, as filed, and to facilitate examination of the application by the International Search Authority.
Applicant hereby requests communication of the enclosed PCT Article amendments to the Designated Offices, in accordance with PCT Article 20(2).
40
PCT/US1998/001149 1997-01-21 1998-01-21 Methods of isolating biological target materials using silica magnetic particles Ceased WO1998031840A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE0895546T DE895546T1 (en) 1997-01-21 1998-01-21 METHOD FOR INSULATING SPECIFIC BIOLOGICAL SUBSTANCES BY MEANS OF MAGNETIC SILICA PARTICLES
AU66475/98A AU732756B2 (en) 1997-01-21 1998-01-21 Methods of isolating biological target materials using silica magnetic particles
JP53467498A JP3253638B2 (en) 1997-01-21 1998-01-21 Separation method of biological target substance using silica magnetic particles
EP98908435A EP0895546B1 (en) 1997-01-21 1998-01-21 Methods of isolating biological target materials using silica magnetic particles
DE69817484T DE69817484T2 (en) 1997-01-21 1998-01-21 METHOD FOR INSULATING SPECIFIC BIOLOGICAL SUBSTANCES BY MEANS OF MAGNETIC SILICA PARTICLES

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/785,097 US6027945A (en) 1997-01-21 1997-01-21 Methods of isolating biological target materials using silica magnetic particles
US08/785,097 1997-01-21

Publications (2)

Publication Number Publication Date
WO1998031840A1 WO1998031840A1 (en) 1998-07-23
WO1998031840B1 true WO1998031840B1 (en) 1998-09-17

Family

ID=25134438

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/001149 Ceased WO1998031840A1 (en) 1997-01-21 1998-01-21 Methods of isolating biological target materials using silica magnetic particles

Country Status (9)

Country Link
US (5) US6027945A (en)
EP (2) EP1367137B1 (en)
JP (1) JP3253638B2 (en)
AU (1) AU732756B2 (en)
BR (1) BR9805897A (en)
CA (1) CA2249393A1 (en)
DE (3) DE69841555D1 (en)
DK (1) DK1367137T3 (en)
WO (1) WO1998031840A1 (en)

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