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WO1998005760A3 - Phosphate starvation-inducible proteins - Google Patents

Phosphate starvation-inducible proteins Download PDF

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Publication number
WO1998005760A3
WO1998005760A3 PCT/CA1997/000532 CA9700532W WO9805760A3 WO 1998005760 A3 WO1998005760 A3 WO 1998005760A3 CA 9700532 W CA9700532 W CA 9700532W WO 9805760 A3 WO9805760 A3 WO 9805760A3
Authority
WO
WIPO (PCT)
Prior art keywords
phosphate
proteins
phosphate starvation
nucleic acids
inducible proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA1997/000532
Other languages
French (fr)
Other versions
WO1998005760A2 (en
Inventor
Daniel D Lefebvre
Mohammad A Malboobi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Queens University at Kingston
Original Assignee
Queens University at Kingston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/688,988 external-priority patent/US6096545A/en
Priority claimed from CA002182421A external-priority patent/CA2182421A1/en
Application filed by Queens University at Kingston filed Critical Queens University at Kingston
Priority to BR9710909A priority Critical patent/BR9710909A/en
Priority to EP97932682A priority patent/EP0917564A2/en
Priority to AU36167/97A priority patent/AU730471B2/en
Publication of WO1998005760A2 publication Critical patent/WO1998005760A2/en
Publication of WO1998005760A3 publication Critical patent/WO1998005760A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

This invention provides proteins, especially protein kinases, glucosidases, and phosphate transporters which are expressed under conditions of phosphate deprivation. Further provided are nucleic acids and nucleic acid constructs encoding these proteins, cells containing the nucleic acids described and transgenic photosynthetic organisms with altered phosphate-inducible enzyme activity.
PCT/CA1997/000532 1996-07-31 1997-07-30 Phosphate starvation-inducible proteins Ceased WO1998005760A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BR9710909A BR9710909A (en) 1996-07-31 1997-07-30 Deoxyribonucleic acid isolated polypeptide isolated nucleic acid isolated recombinant expression vector cell plant seed tissue culture photosynthetic organism prokaryotic cell descending method to produce a plant method antibody to detect the expression of a protein kinase method to detect the expression of a b-glucosity nucleic acid sequence and method to change the tolerance to cold or freezing in a plant
EP97932682A EP0917564A2 (en) 1996-07-31 1997-07-30 Phosphate starvation-inducible proteins
AU36167/97A AU730471B2 (en) 1996-07-31 1997-07-30 Phosphate starvation-inducible proteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/688,988 US6096545A (en) 1996-07-31 1996-07-31 Phosphate starvation-inducible proteins
CA2,182,421 1996-07-31
CA002182421A CA2182421A1 (en) 1996-07-31 1996-07-31 Phosphate starvation-inducible proteins
US08/688,988 1996-07-31

Publications (2)

Publication Number Publication Date
WO1998005760A2 WO1998005760A2 (en) 1998-02-12
WO1998005760A3 true WO1998005760A3 (en) 1998-10-08

Family

ID=25678591

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1997/000532 Ceased WO1998005760A2 (en) 1996-07-31 1997-07-30 Phosphate starvation-inducible proteins

Country Status (5)

Country Link
EP (1) EP0917564A2 (en)
CN (1) CN1226925A (en)
AU (1) AU730471B2 (en)
BR (1) BR9710909A (en)
WO (1) WO1998005760A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922564A (en) 1997-02-24 1999-07-13 Performance Plants, Inc. Phosphate-deficiency inducible promoter
US6433249B1 (en) * 1998-11-10 2002-08-13 Pioneer Hi-Bred International, Inc. Use of β-glucosidase to enhance disease resistance and resistance to insects in crop plants
KR100401007B1 (en) * 2000-08-11 2003-10-08 윤성중 Phosphate transporter cDNA from tobacco (Nicotiana tabacum L)
US20070011772A1 (en) * 2002-03-06 2007-01-11 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Polynucleotides encoding a beta-glucosidase and uses thereof
US20090271895A1 (en) * 2004-07-16 2009-10-29 Cropdesign N.V. Plants having improved growth characteristics and method for making the same
EP2543732A1 (en) * 2006-08-02 2013-01-09 CropDesign N.V. Plants transformed with a nucleic acid encoding chloroplastic fructose-1,6-bisphosphatase having increased yield, and a method for making the same
US9055752B2 (en) 2008-11-06 2015-06-16 Intercontinental Great Brands Llc Shelf-stable concentrated dairy liquids and methods of forming thereof
UA112972C2 (en) 2010-09-08 2016-11-25 Інтерконтінентал Грейт Брендс ЛЛС LIQUID DAIRY CONCENTRATE WITH A HIGH CONTENT OF DRY SUBSTANCES
US9709551B2 (en) 2013-01-29 2017-07-18 Creative Scientist, Inc. Method for determining the sensitivity of an individual to low dose ionizing radiation
CN105219852B (en) * 2015-09-25 2019-07-02 南京大学 A kind of primer and application and method for detecting gene expression level of phosphorus metabolism-related enzymes in Anabaena sp.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5413920A (en) * 1992-04-08 1995-05-09 Purdue Research Foundation Method for enhanced production and recovery of phosphate starvation inducible gene products
WO1997035984A1 (en) * 1996-03-25 1997-10-02 Mitsui Plant Biotechnology Research Institute Plant phosphate transporter gene and method of regulating plant growth with said gene
WO1998004701A1 (en) * 1996-07-29 1998-02-05 Purdue Research Foundation Methods and compositions for improving a plant's ability to take in phosphate from soil

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5413920A (en) * 1992-04-08 1995-05-09 Purdue Research Foundation Method for enhanced production and recovery of phosphate starvation inducible gene products
WO1997035984A1 (en) * 1996-03-25 1997-10-02 Mitsui Plant Biotechnology Research Institute Plant phosphate transporter gene and method of regulating plant growth with said gene
WO1998004701A1 (en) * 1996-07-29 1998-02-05 Purdue Research Foundation Methods and compositions for improving a plant's ability to take in phosphate from soil

Non-Patent Citations (18)

* Cited by examiner, † Cited by third party
Title
B.L. EPEL ET AL.,: "A 41kDa protein isolated from maize mesocotyl cell walls immunolocalizes to plamodesmata", PROTOPLASMA, vol. 191, 1996, pages 70 - 78, XP002071949 *
C. FIFE AND D. LEFEBVRE: "Protein profiles of Brassica-nigra suspension cultures in response to phosphate stress", PLANT PHYSIOLOGY, vol. 89, no. 4 suppl., 1989, BETHESDA, MD, US, pages 178, XP002060425 *
C.A. FIFE ET AL.,: "The effect of phosphate deprivation on protein synthesis and fixed carbon storage reserves in Brassica nigra suspension cells", CANADIAN JOURNAL OF BOTANICS, vol. 68, 1990, OTTAWA, ONTARIO, CA, pages 1840 - 1847, XP002060427 *
DATABASE EMBL NUCLEOTIDE SEQUENCE, 1 November 1996 (1996-11-01), HINXTON, GB, XP002070419 *
DATABASE EMBL NUCLEOTIDE SEQUENCE, HINXTON, GB, XP002071153 *
DATABASE WPI Week 9745, 2 January 1997 Derwent World Patents Index; AN 97-489647, XP002070415 *
G. LEGGEWIE ET AL.,: "Two cDNA's from potato are able to complement a phosphate uptake deficient yeast mutant: Identification of phosphate transporters from higher plants", PLANT CELL., vol. 9, 1997, MD US, pages 381 - 392, XP002070420 *
M.A. MALBOOBI AND D.D. LEFEBVRE: "A phosphate-starvation inducible beta-glucosidase gene (psr3.2) isolated from Arabidopsis thaliana is a member of a distinct subfamily of the BGA family", PLANT MOLECULAR BIOLOGY, vol. 34, no. 1, 1997, DORDRECHT, NL, pages 57 - 68, XP002061130 *
M.A. MALBOOBI AND D.D. LEFEBVRE: "Differential gene expression at the transcriptional level in response to phosphate starvation in Brassica-nigra suspension cells", PLANT PHYSIOLOGY, vol. 102, no. 1 suppl., - 1993, ROCKVILLE, US, pages 9, XP002060426 *
M.A. MALBOOBI AND D.D. LEFEBVRE: "Isolation of cDNA clones of genes with altered expression levels in phosphate-starved Brassica nigra suspension cells", PLANT MOLECULAR BIOLOGY, vol. 28, no. 5, 1995, BE, pages 859 - 870, XP002061861 *
M.A. MALBOOBI ET AL.,: "Identification and nucleotide sequences of cDNA clones of phosphate inducible beta-glucosidase genes of Brassicaceae (Accession Nos. U72153 and U72154) (PGR96-114)", PLANT PHYSIOLOGY, vol. 112, 26 August 1996 (1996-08-26), pages 1399 - 1399, XP002061129 *
M.J. HAWKESFORD AND A.R. BELCHER: "Differential protein synthesis in response to sulphate and phosphate deprivation: Identification of possible components of plasma-membrane transport systems in cultured tomato roots", PLANTA, vol. 185, no. 3, 1991, BERLIN, DE, pages 323 - 329, XP002070416 *
N. MITSUKAWA ET AL.,: "Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 94, 1997, WASHINGTON, DC, US, pages 7098 - 7102, XP002070505 *
P. BUDICKY ET AL.,: "Identification of a putative phosphate starvation inducible protein kinase from Brassica nigra", PLANT PHYSIOLOGY, vol. 102, no. 1 suppl., - 1993, ROCKVILLE, US, pages 68, XP002060422 *
T. NEWMAN ET AL.,: "Genes galore: a summary of methods for accessing results from large-scale partial sequencing of anonymous Arabidopsis cDNA clones", PLANT PHYSIOLOGY, vol. 106, 1994, pages 1241 - 1255, XP002004690 *
U.S. MUCHHAL ET AL.,: "Phosphate transporters from the higher plant Arabidopsis thaliana", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 93, no. 19, 1996, WASHINGTON, DC, US, pages 10519 - 10523, XP002070417 *
Y.S. PARK ET AL.,: "Frequent in-frame lenght variations are found in the diverged simple repeat sequences of the protein-coding regions of two putative protein kinase genes of Brassica napus", PLANT MOLECULAR BIOLOGY, vol. 27, 1995, DORDRECHT, NL, pages 829 - 833, XP002060428 *
Y.S. PARK ET AL.,: "Two putative protein kinases from Arabidopsis thaliana contain highly acidic domains", PLANT MOLECULAR BIOLOGY, vol. 22, 1993, DORDRECHT, NL, pages 615 - 624, XP002060429 *

Also Published As

Publication number Publication date
WO1998005760A2 (en) 1998-02-12
AU730471B2 (en) 2001-03-08
CN1226925A (en) 1999-08-25
BR9710909A (en) 1999-08-17
EP0917564A2 (en) 1999-05-26
AU3616797A (en) 1998-02-25

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