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WO1997039143A3 - Procede pour identifier de nouveaux agents antifongiques - Google Patents

Procede pour identifier de nouveaux agents antifongiques Download PDF

Info

Publication number
WO1997039143A3
WO1997039143A3 PCT/US1997/006304 US9706304W WO9739143A3 WO 1997039143 A3 WO1997039143 A3 WO 1997039143A3 US 9706304 W US9706304 W US 9706304W WO 9739143 A3 WO9739143 A3 WO 9739143A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
identification
phosphorylation
antifungal agents
kinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/006304
Other languages
English (en)
Other versions
WO1997039143A2 (fr
Inventor
Jennifer Marion Fostel
Mark Edward Winey
Estelle Marthe Steiner
Francis Conrad Luca
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
University of Technology Corp
Original Assignee
Abbott Laboratories
University of Technology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories, University of Technology Corp filed Critical Abbott Laboratories
Publication of WO1997039143A2 publication Critical patent/WO1997039143A2/fr
Publication of WO1997039143A3 publication Critical patent/WO1997039143A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes de criblage permettant d'identifier des agents antifongiques. Dans lesdites méthodes (a) on combine une protéine kinase de point de contrôle appropriée à un composé test; (b) on catalyse la phosphorylation d'un substrat à base de kinase, la kinase et le composé test étant dans des conditions appropriées pour ladite phosphorylation; et (c) on détermine le degré de phosphorylation du substrat. L'invention concerne également des polypeptides et des protéines de fusion utiles pour lesdites méthodes, ainsi que des ADN plasmidiques et des cellules hôtes transformées utiles pour la préparation desdits polypeptides et protéines de fusion.
PCT/US1997/006304 1996-04-17 1997-04-17 Procede pour identifier de nouveaux agents antifongiques Ceased WO1997039143A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63361796A 1996-04-17 1996-04-17
US08/633,617 1996-04-17

Publications (2)

Publication Number Publication Date
WO1997039143A2 WO1997039143A2 (fr) 1997-10-23
WO1997039143A3 true WO1997039143A3 (fr) 1998-05-14

Family

ID=24540386

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/006304 Ceased WO1997039143A2 (fr) 1996-04-17 1997-04-17 Procede pour identifier de nouveaux agents antifongiques

Country Status (1)

Country Link
WO (1) WO1997039143A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6770398A (en) * 1997-03-28 1998-10-22 Mycotox, Inc. Histidine kinase assay
US6670167B1 (en) 1999-11-01 2003-12-30 Agouron Pharmaceuticals, Inc. Catalytic domain of the human effector cell cycle checkpoint protein kinase materials and methods for identification of inhibitors thereof
DE10356218A1 (de) * 2003-12-03 2005-06-30 Bayer Cropscience Ag Verfahren zum Identifizieren von fungizid wirksamen Verbindungen basierend auf Pyruvatkinasen aus Pilzen

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0446798A1 (fr) * 1990-03-13 1991-09-18 Hoechst-Roussel Pharmaceuticals Incorporated Alkyl-1, alcényl-1 et alkynylaryl-1 amino-2 propanediols-1,3 et composés apparentés, procédé et produits intermédiaires pour leur préparation et leur application comme médicaments
EP0626454A1 (fr) * 1993-05-25 1994-11-30 American Cyanamid Company Screening de corps à pôle-fuseau par les fongicides
WO1995023612A1 (fr) * 1994-03-04 1995-09-08 Promega Corporation Quantification de l'activite d'une proteine-kinase individuelle

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0446798A1 (fr) * 1990-03-13 1991-09-18 Hoechst-Roussel Pharmaceuticals Incorporated Alkyl-1, alcényl-1 et alkynylaryl-1 amino-2 propanediols-1,3 et composés apparentés, procédé et produits intermédiaires pour leur préparation et leur application comme médicaments
EP0626454A1 (fr) * 1993-05-25 1994-11-30 American Cyanamid Company Screening de corps à pôle-fuseau par les fongicides
WO1995023612A1 (fr) * 1994-03-04 1995-09-08 Promega Corporation Quantification de l'activite d'une proteine-kinase individuelle

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FRIEDMAN ET AL: "The 110-kD spindle pole body component of Saccharomyces cerevisiae is a phosphoprotein that is modified in a cell cycle-dependent manner.", THE JOURNAL OF CELL BIOLOGY, vol. 132, no. 5, March 1996 (1996-03-01), pages 903 - 914, XP002057395 *
LAUZE AL: "Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase.", EMBO JOURNAL, vol. 14, no. 8, 18 April 1995 (1995-04-18), pages 1655 - 1663, XP002057393 *
POCH ET AL.: "RPK1, an essential yeast protein kinase involved in the regulation of the onset of mitosis, shows homology to mammalian dual-specificty kinases", MOLECULAR & GENERAL GENETICS, vol. 243, 1994, BERLIN, pages 641 - 653, XP002057394 *

Also Published As

Publication number Publication date
WO1997039143A2 (fr) 1997-10-23

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