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WO1997032992B1 - Lignees cellulaires hematopoietiques immortalisees, systeme cellulaire de ces lignees avec des cellules du stroma, applications in vitro, ex vivo et in vivo, et generation in vivo de cellules dendritiques et de macrophages - Google Patents

Lignees cellulaires hematopoietiques immortalisees, systeme cellulaire de ces lignees avec des cellules du stroma, applications in vitro, ex vivo et in vivo, et generation in vivo de cellules dendritiques et de macrophages

Info

Publication number
WO1997032992B1
WO1997032992B1 PCT/US1997/003186 US9703186W WO9732992B1 WO 1997032992 B1 WO1997032992 B1 WO 1997032992B1 US 9703186 W US9703186 W US 9703186W WO 9732992 B1 WO9732992 B1 WO 9732992B1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
stem cells
hematopoietic stem
cell
hematopoietic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/003186
Other languages
English (en)
Other versions
WO1997032992A1 (fr
Filing date
Publication date
Priority claimed from US08/612,302 external-priority patent/US5811297A/en
Application filed filed Critical
Priority to EP97915851A priority Critical patent/EP0954594A2/fr
Priority to JP9531851A priority patent/JP2000508885A/ja
Publication of WO1997032992A1 publication Critical patent/WO1997032992A1/fr
Publication of WO1997032992B1 publication Critical patent/WO1997032992B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

Cette invention concerne des lignées cellulaires hématopoïétiques ayant une durée de vie plus longue, notamment des lignées cellulaires du stroma utiles dans la conservation in vitro de cellules souches hématopoïétiques pluripotentielles non différentiées. Les cellules souches immortalisées différentiées et non différentiées peuvent être utilisées dans la transplantation de moelle osseuse, dans des applications de thérapie génique et de thérapie cellulaire, ainsi que dans des systèmes de modèles in vitro afin de découvrir des médicaments ou d'effectuer des analyses toxicologiques.
PCT/US1997/003186 1996-03-07 1997-03-07 Lignees cellulaires hematopoietiques immortalisees, systeme cellulaire de ces lignees avec des cellules du stroma, applications in vitro, ex vivo et in vivo, et generation in vivo de cellules dendritiques et de macrophages Ceased WO1997032992A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP97915851A EP0954594A2 (fr) 1996-03-07 1997-03-07 Lignees cellulaires hematopoietiques immortalisees, systeme cellulaire de ces lignees avec des cellules du stroma, applications in vitro, ex vivo et in vivo, et generation in vivo de cellules dendritiques et de macrophages
JP9531851A JP2000508885A (ja) 1996-03-07 1997-03-07 不死化造血細胞系、該細胞系とストロマ細胞との細胞システム、ｉｎ ｖｉｔｒｏ、ｅｘ ｖｉｖｏ および ｉｎ ｖｉｖｏ における使用、ならびに樹状細胞およびマクロファージの ｉｎ ｖｉｔｒｏ 産生

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/612,302 1996-03-07
US08/612,302 US5811297A (en) 1996-03-07 1996-03-07 Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages

Publications (2)

Publication Number Publication Date
WO1997032992A1 WO1997032992A1 (fr) 1997-09-12
WO1997032992B1 true WO1997032992B1 (fr) 1997-11-06

Family

ID=24452603

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/003186 Ceased WO1997032992A1 (fr) 1996-03-07 1997-03-07 Lignees cellulaires hematopoietiques immortalisees, systeme cellulaire de ces lignees avec des cellules du stroma, applications in vitro, ex vivo et in vivo, et generation in vivo de cellules dendritiques et de macrophages

Country Status (5)

Country Link
US (1) US5811297A (fr)
EP (1) EP0954594A2 (fr)
JP (1) JP2000508885A (fr)
CA (1) CA2248555A1 (fr)
WO (1) WO1997032992A1 (fr)

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AU778844B2 (en) * 1998-12-09 2004-12-23 Vistagen, Inc. Toxicity typing using embryoid bodies
CA2367692A1 (fr) * 1999-03-15 2000-09-21 Introgen Therapeutics, Inc. Cellules dendritiques transduites avec un gene du soi de type sauvage suscitant des reponses immunitaires antitumorales puissantes
ATE463578T1 (de) 1999-04-14 2010-04-15 Novartis Vaccines & Diagnostic Zusammensetzungen und verfahren zur auslösung einer immunantwort auf basis alphavirus-vektoren- systeme
AU5178800A (en) * 1999-06-01 2000-12-18 Cornell Research Foundation Inc. Activation of dendritic cells to enhance immunity
DE60140120D1 (de) 2000-06-14 2009-11-19 Vistagen Inc Toxizitätstypisierung unter verwendung von leberstammzellen
KR20030009531A (ko) * 2000-06-14 2003-01-29 비스타겐 인코포레이티드 중간엽 줄기 세포를 사용하는 독성 타이핑
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US20030027334A1 (en) * 2000-12-13 2003-02-06 Fitzpatrick David R. Method for generating immortal dendritic cell lines
DE10139428A1 (de) 2001-08-17 2003-03-27 Nemod Immuntherapie Ag Herstellung und Verwendung von humanen CD124 und CD116 positiven Tumorzelllinien zur Herstellung von allogenen oder semi-allogenen Immuntherapeutika
JPWO2003038076A1 (ja) * 2001-10-31 2005-02-24 株式会社レノメディクス研究所 不死化間葉系細胞及びその利用
EP1451300A4 (fr) * 2001-11-09 2007-01-10 Artecel Sciences Inc Methodes et compositions dans lesquelles on utilise des cellules stromales pour supporter des cellules souches embryonnaires et adultes
JP2005514071A (ja) * 2001-12-21 2005-05-19 ザ ユナイテッド ステイツ オブ アメリカ レプレゼンティッド バイ ザ セクレタリー オブ ザ ユー．エス． デパートメント オブ ザ ネイビー 内皮細胞との前培養による遺伝子導入効率の上昇
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US10894944B2 (en) * 2009-04-10 2021-01-19 Monash University Cell culture media
EP3633025B1 (fr) 2009-11-12 2022-09-14 Technion Research & Development Foundation Ltd. Milieu de culture, cultures de cellules et procédés de culture de cellules souches pluripotentes dans un état indifférencié
WO2012061146A1 (fr) 2010-10-25 2012-05-10 The Children's Hospital Of Philadelphia Compositions et procédés de génération de plaquettes et leurs procédés d'utilisation
JP2016516758A (ja) * 2013-04-09 2016-06-09 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 骨髄血球減少症及び関連する合併症を予防又は治療するためのｍ−ｃｓｆの使用
US10987670B2 (en) 2015-04-14 2021-04-27 President And Fellows Of Harvard College Electrode array for vortex-assisted electroporation
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EP0563485A1 (fr) * 1992-03-30 1993-10-06 Schering-Plough Génération in vitro de cellules dendritiques humaines et leurs usages
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US5670347A (en) * 1994-05-11 1997-09-23 Amba Biosciences Llc Peptide-mediated gene transfer
US6103522A (en) * 1994-07-20 2000-08-15 Fred Hutchinson Cancer Research Center Human marrow stromal cell lines which sustain hematopoiesis

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