WO1997012975B1 - An epithelial protein and dna thereof for use in early cancer detection - Google Patents
An epithelial protein and dna thereof for use in early cancer detectionInfo
- Publication number
- WO1997012975B1 WO1997012975B1 PCT/US1996/015825 US9615825W WO9712975B1 WO 1997012975 B1 WO1997012975 B1 WO 1997012975B1 US 9615825 W US9615825 W US 9615825W WO 9712975 B1 WO9712975 B1 WO 9712975B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- probe
- epithelial
- labeled
- nucleic acid
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Abstract
The present invention is a purified and isolated epithelial protein, peptide and variants thereof whose increased presence in an epithelial cell is indicative of precancer. One epithelial protein which is an early detection marked for lung cancer was purified from two human lung cancer cell lines, NCI-H720 and NCI-H157. Using a six-step procedure, the epithelial protein was purified using a Western blot detection system under both non-reducing and reducing conditions. Purification steps included anion exchange chromatography, preparative isoelectric focusing, polymer-based C18HPLC and analytic C4HPLC. After an approximately 25,000 fold purification the immunostaining protein was >90 % pure as judged by coomassie blue staining after reducing SDS-PAGE. The primary epithelial protein share some sequence homology with the heterogeneous nuclear ribonucleoprotein (hnRNP) A2. A minor co-purifying epithelial protein shares some sequence homology with the splice variant hnRNP-B1. Molecular analysis of primary normal bronchial epithelial cell cultures demonstrated a low level the epithelial protein expression, consistent with immunohistochemical staining of clinical samples, and an increased level of expression in most lung cancer cells. The epithelial protein is a marker of epithelial transformation in lung, breast, bone, ovary, prostate, kidney, melanoma and myeloma and may be casual in the process of carcinogenesis. Methods are provided for monitoring the expression of the epithelial protein, peptides and variants using molecular and immunological techniques as a screen for precancer and cancer in mammals. A method of computerized diagnoses of cancer and precancer is provided which detects levels of hnRNP messenger RNA.
Claims
AMENDED CLAIMS
[received by the International Bureau on 21 April 1997 (21.04.97); new claims 59-67 added; remaining claims unchanged (2 pages)]
54. The method according to claim 53 wherein the optical image o is a spatial electronic array.
55. The method according to claim 53 wherein the image is acquired at two different wavelengths.
5
56. The method according to claim 53 wherein the parameter unique to atypical cells is selected from the group consisting of nuclear texture, nuclear ellipse area, optical density, hnRNP mRNA and combinations thereof.
0
57. The method according to claim 53 wherein the cell is treated with a labeled probe, said probe specifically hybridized with hnRNP mRNA.
58. The method according to claim 53 wherein the known 5 negative and known atypical cells are from an archived bank of cells taken from normal humans and humans with cancer or precancer.
59. A labeled nucleic acid sequence probe suitable for use in the o method according to claim 12.
60. A labeled nucleotide probe according to claim 59 comprising a sequence capable of specifically hybridizing with hnRNP or RNA.
5
61. The probes of claim 59 or claim 60, wherein said probe is labeled with digoxigenin.
62. The labeled probe of claim 59 or 60 complexed with a nucleic acid sequence complimentary to the labeled probe.
63. The labeled probe of claim 61 employed with a nucleic acid sequence complimentary to the labeled probe.
-148-
o 64. The labeled probe of claim 62 complexed with a nucleic acid sequence complimentary to the labeled probe, and further comprising an antibody complexed to the label on said probe.
65. The labeled probe of claim 64 wherein said antibody is an 5 anti digoxigenin antibody.
66. The nucleic acid sequence probe of claim 12 or claim 43 complexed with a nucleic acid sequence complimentary to said probe.
67. The nucleic acid probes of claims 59, 60 and 66 having the sequence provided in SEQ ID NO: 11 or SEQ ID NO: 18.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96936141A EP0861323A1 (en) | 1995-10-02 | 1996-10-02 | An epithelial protein and dna thereof for use in early cancer detection |
| JP9514401A JP2000500322A (en) | 1995-10-02 | 1996-10-02 | Epithelial proteins and their DNA for use in early cancer detection |
| AU73864/96A AU7386496A (en) | 1995-10-02 | 1996-10-02 | An epithelial protein and dna thereof for use in early cancer detection |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/538,711 US5994062A (en) | 1995-10-02 | 1995-10-02 | Epithelial protein and DNA thereof for use in early cancer detection |
| US08/725,027 | 1996-10-02 | ||
| US08/725,027 US6251586B1 (en) | 1995-10-02 | 1996-10-02 | Epithelial protein and DNA thereof for use in early cancer detection |
| US08/538,711 | 1996-10-02 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1997012975A1 WO1997012975A1 (en) | 1997-04-10 |
| WO1997012975B1 true WO1997012975B1 (en) | 1997-05-22 |
Family
ID=27065902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1996/015825 Ceased WO1997012975A1 (en) | 1995-10-02 | 1996-10-02 | An epithelial protein and dna thereof for use in early cancer detection |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6251586B1 (en) |
| EP (2) | EP0861323A1 (en) |
| JP (1) | JP2000500322A (en) |
| AU (1) | AU7386496A (en) |
| WO (1) | WO1997012975A1 (en) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5914238A (en) | 1996-06-05 | 1999-06-22 | Matritech, Inc. | Materials and methods for detection of breast cancer |
| WO1998014469A2 (en) * | 1996-10-02 | 1998-04-09 | The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services | An epithelial protein and dna thereof for use in early cancer detection |
| US6025470A (en) * | 1997-06-23 | 2000-02-15 | Ludwig Institute For Cancer Research | Isolated nona- and decapeptides which bind to HLA molecules, and the use thereof |
| EP1310556A3 (en) * | 1998-03-31 | 2003-07-23 | Genzyme Corporation | Composition and methods for the identification of lung tumor cells |
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| GB2354241A (en) | 1999-09-17 | 2001-03-21 | Rupert Donald Holms | Regulatory/unfolding peptides of ezrin |
| WO2001021649A2 (en) * | 1999-09-22 | 2001-03-29 | Cedars-Sinai Medical Center | Nucleic acids encoding vitamin d response element binding proteins, products related thereto, and methods of using same |
| US7970718B2 (en) * | 2001-05-18 | 2011-06-28 | Health Discovery Corporation | Method for feature selection and for evaluating features identified as significant for classifying data |
| EP1280901B1 (en) * | 2000-03-29 | 2005-01-26 | Max-Planck-Gesellschaft | 3d structure of polypeptides containing a tpr-structure motif with chaperone-binding function, crystals thereof and compounds for inhibition of said peptides |
| US6519355B2 (en) * | 2001-03-28 | 2003-02-11 | Alan C. Nelson | Optical projection imaging system and method for automatically detecting cells having nuclear and cytoplasmic densitometric features associated with disease |
| US6522775B2 (en) | 2001-03-28 | 2003-02-18 | Alan C. Nelson | Apparatus and method for imaging small objects in a flow stream using optical tomography |
| US20060023219A1 (en) * | 2001-03-28 | 2006-02-02 | Meyer Michael G | Optical tomography of small objects using parallel ray illumination and post-specimen optical magnification |
| US7907765B2 (en) | 2001-03-28 | 2011-03-15 | University Of Washington | Focal plane tracking for optical microtomography |
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| US6944322B2 (en) | 2001-03-28 | 2005-09-13 | Visiongate, Inc. | Optical tomography of small objects using parallel ray illumination and post-specimen optical magnification |
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| US6741730B2 (en) | 2001-08-10 | 2004-05-25 | Visiongate, Inc. | Method and apparatus for three-dimensional imaging in the fourier domain |
| US6636623B2 (en) * | 2001-08-10 | 2003-10-21 | Visiongate, Inc. | Optical projection imaging system and method for automatically detecting cells with molecular marker compartmentalization associated with malignancy and disease |
| US6697509B2 (en) | 2001-10-04 | 2004-02-24 | Chromavision Medical Systems, Inc. | Method and apparatus for scoring the uptake of markers in cells |
| US7260253B2 (en) * | 2002-04-19 | 2007-08-21 | Visiongate, Inc. | Method for correction of relative object-detector motion between successive views |
| US7738945B2 (en) * | 2002-04-19 | 2010-06-15 | University Of Washington | Method and apparatus for pseudo-projection formation for optical tomography |
| US20050085708A1 (en) * | 2002-04-19 | 2005-04-21 | University Of Washington | System and method for preparation of cells for 3D image acquisition |
| US7811825B2 (en) * | 2002-04-19 | 2010-10-12 | University Of Washington | System and method for processing specimens and images for optical tomography |
| US7197355B2 (en) | 2002-04-19 | 2007-03-27 | Visiongate, Inc. | Variable-motion optical tomography of small objects |
| US6697508B2 (en) | 2002-05-10 | 2004-02-24 | Visiongate, Inc. | Tomographic reconstruction of small objects using a priori knowledge |
| US6770893B2 (en) * | 2002-05-13 | 2004-08-03 | Visiongate, Inc. | Method and apparatus for emission computed tomography using temporal signatures |
| US7687167B2 (en) * | 2003-07-18 | 2010-03-30 | Panasonic Corporation | Power supply unit |
| US7229778B2 (en) | 2004-02-26 | 2007-06-12 | The Procter & Gamble Company | Methods for determining the relative benefits and/or evaluating quantitative changes of products on epithelial tissue |
| JPWO2006011667A1 (en) * | 2004-07-30 | 2008-05-01 | 東ソー株式会社 | Method for measuring heteronuclear ribonucleotide protein B1 (hnRNPB1) mRNA |
| US6991738B1 (en) | 2004-10-13 | 2006-01-31 | University Of Washington | Flow-through drum centrifuge |
| US20060096358A1 (en) * | 2004-10-28 | 2006-05-11 | University Of Washington | Optical projection tomography microscope |
| US7494809B2 (en) * | 2004-11-09 | 2009-02-24 | Visiongate, Inc. | Automated cell sample enrichment preparation method |
| US20070091109A1 (en) * | 2005-09-13 | 2007-04-26 | Roscoe Atkinson | Image quality |
| JP4915071B2 (en) * | 2005-09-22 | 2012-04-11 | 株式会社ニコン | Microscope and virtual slide creation system |
| US20070196935A1 (en) * | 2006-02-17 | 2007-08-23 | Taiwan Semiconductor Manufacturing Company, Ltd. | Prediction of ESL/ILD remaining thickness |
| US7835561B2 (en) | 2007-05-18 | 2010-11-16 | Visiongate, Inc. | Method for image processing and reconstruction of images for optical tomography |
| US7787112B2 (en) | 2007-10-22 | 2010-08-31 | Visiongate, Inc. | Depth of field extension for optical tomography |
| US8143600B2 (en) * | 2008-02-18 | 2012-03-27 | Visiongate, Inc. | 3D imaging of live cells with ultraviolet radiation |
| US8090183B2 (en) | 2009-03-12 | 2012-01-03 | Visiongate, Inc. | Pattern noise correction for pseudo projections |
| US8254023B2 (en) * | 2009-02-23 | 2012-08-28 | Visiongate, Inc. | Optical tomography system with high-speed scanner |
| IN2012DN02336A (en) | 2009-10-19 | 2015-08-21 | Ventana Med Syst Inc | |
| US8492325B2 (en) | 2010-03-01 | 2013-07-23 | The Procter & Gamble Company | Dual-usage liquid laundry detergents comprising a silicone anti-foam |
| EP2582842A2 (en) | 2010-06-16 | 2013-04-24 | Yissum Research Development Company of The Hebrew University of Jerusalem | Method of diagnosing and treating cancer |
| US8113412B1 (en) | 2011-05-13 | 2012-02-14 | Taiwan Semiconductor Manufacturing Company, Ltd | Methods for detecting defect connections between metal bumps |
| CA2955499A1 (en) | 2014-08-07 | 2016-02-11 | The Procter & Gamble Company | Soluble unit dose comprising a laundry detergent composition |
| US11069054B2 (en) | 2015-12-30 | 2021-07-20 | Visiongate, Inc. | System and method for automated detection and monitoring of dysplasia and administration of immunotherapy and chemotherapy |
| US11214613B2 (en) | 2017-05-30 | 2022-01-04 | The University Of British Columbia | Epitopes in the RNA recognition motif 1 (RRM1) of TDP-43 and misfolding-selective antibodies thereto |
| US20200306408A1 (en) | 2019-03-28 | 2020-10-01 | The Procter & Gamble Company | Cyclodextrin containing freshening composition |
| US12404478B2 (en) | 2019-10-15 | 2025-09-02 | The Procter & Gamble Company | Detergent compositions |
| CN113936738B (en) * | 2021-12-14 | 2022-04-22 | 鲁东大学 | RNA-protein binding site prediction method based on convolutional neural network |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4569788A (en) | 1983-05-18 | 1986-02-11 | The United States Of America As Represented By The Department Of Health And Human Services | Monoclonal antibodies against non small cell lung cancer |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| AU632469B2 (en) | 1988-04-04 | 1993-01-07 | Johns Hopkins University, The | A method for early detection of lung cancer |
| US5194599A (en) | 1988-09-23 | 1993-03-16 | Gilead Sciences, Inc. | Hydrogen phosphonodithioate compositions |
| US5256775A (en) | 1989-06-05 | 1993-10-26 | Gilead Sciences, Inc. | Exonuclease-resistant oligonucleotides |
| US5264562A (en) | 1989-10-24 | 1993-11-23 | Gilead Sciences, Inc. | Oligonucleotide analogs with novel linkages |
-
1996
- 1996-10-02 WO PCT/US1996/015825 patent/WO1997012975A1/en not_active Ceased
- 1996-10-02 EP EP96936141A patent/EP0861323A1/en not_active Withdrawn
- 1996-10-02 EP EP03078938A patent/EP1426381A1/en not_active Withdrawn
- 1996-10-02 AU AU73864/96A patent/AU7386496A/en not_active Abandoned
- 1996-10-02 US US08/725,027 patent/US6251586B1/en not_active Expired - Fee Related
- 1996-10-02 JP JP9514401A patent/JP2000500322A/en active Pending
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