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WO1997010328A3 - Compositions and method for modulation of gene expression in plants - Google Patents

Compositions and method for modulation of gene expression in plants Download PDF

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Publication number
WO1997010328A3
WO1997010328A3 PCT/US1996/011689 US9611689W WO9710328A3 WO 1997010328 A3 WO1997010328 A3 WO 1997010328A3 US 9611689 W US9611689 W US 9611689W WO 9710328 A3 WO9710328 A3 WO 9710328A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
modulation
compositions
gene expression
acid molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/011689
Other languages
French (fr)
Other versions
WO1997010328A2 (en
Inventor
Michael G Zwick
Brent E Edington
James A Mcswiggen
Patricia Ann Owens Merlo
Lining Guo
Thomas A Skokut
Scott A Young
Otto Folkerts
Donald J Merlo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sirna Therapeutics Inc
Corteva Agriscience LLC
Original Assignee
DowElanco LLC
Ribozyme Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DowElanco LLC, Ribozyme Pharmaceuticals Inc filed Critical DowElanco LLC
Priority to MX9800454A priority Critical patent/MX9800454A/en
Priority to BR9610402-3A priority patent/BR9610402A/en
Priority to AU67617/96A priority patent/AU6761796A/en
Priority to EP96927999A priority patent/EP0842286A2/en
Priority to JP9506989A priority patent/JPH11509733A/en
Publication of WO1997010328A2 publication Critical patent/WO1997010328A2/en
Publication of WO1997010328A3 publication Critical patent/WO1997010328A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

An enzymatic nucleic acid molecule with RNA cleaving activity, wherein said nucleic acid molecule modulates the expression of a gene in a plant. A transgenic plant comprising nucleic acids encoding for an enzymatic nucleic acid molecule with RNA cleaving activity, wherein said nucleic acid molecule modulates the expression of a gene in said plant.
PCT/US1996/011689 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants Ceased WO1997010328A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
MX9800454A MX9800454A (en) 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants.
BR9610402-3A BR9610402A (en) 1995-07-13 1996-07-12 Compositions and method for modulating gene expression in plants.
AU67617/96A AU6761796A (en) 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants
EP96927999A EP0842286A2 (en) 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants
JP9506989A JPH11509733A (en) 1995-07-13 1996-07-12 Compositions and methods for regulating gene expression in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US113595P 1995-07-13 1995-07-13
US60/001,135 1995-07-13

Publications (2)

Publication Number Publication Date
WO1997010328A2 WO1997010328A2 (en) 1997-03-20
WO1997010328A3 true WO1997010328A3 (en) 1997-05-15

Family

ID=21694555

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/011689 Ceased WO1997010328A2 (en) 1995-07-13 1996-07-12 Compositions and method for modulation of gene expression in plants

Country Status (8)

Country Link
EP (1) EP0842286A2 (en)
JP (1) JPH11509733A (en)
CN (1) CN1196091A (en)
AU (1) AU6761796A (en)
BR (1) BR9610402A (en)
CA (1) CA2226728A1 (en)
MX (1) MX9800454A (en)
WO (1) WO1997010328A2 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19601365A1 (en) 1996-01-16 1997-07-17 Planttec Biotechnologie Gmbh Nucleic acid molecules from plants encoding enzymes involved in starch synthesis
AU5918398A (en) * 1997-01-28 1998-08-18 Ribozyme Pharmaceuticals, Inc. Compositions and method for modulation of alkaloid biosynthesis and flower formation in plants
WO1998043993A2 (en) * 1997-03-31 1998-10-08 Yale University Nucleic acid catalysts
US6656731B1 (en) 1997-09-22 2003-12-02 Max Planck Gesellschaft Zur Forderung Der Wissenschaften E.V. Nucleic acid catalysts with endonuclease activity
WO1999016871A2 (en) * 1997-09-22 1999-04-08 Max-Planck-Gesellschaft Zur Forderung Der Wissensc Nucleic acid catalysts with endonuclease activity
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
US6426185B1 (en) 1998-01-16 2002-07-30 Large Scale Biology Corporation Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation
EP3214177A3 (en) * 1998-04-08 2017-11-22 Commonwealth Scientific and Industrial Research Organisation Methods and means for obtaining modified phenotypes
US7008664B1 (en) * 1998-06-11 2006-03-07 E. I. Du Pont De Nemours And Company Method for improving the carcass quality of an animal
CA2334161A1 (en) * 1998-07-20 2000-01-27 Ribozyme Pharmaceuticals, Inc. Use of nucleic acid molecules as antiviral agents
AU775017B2 (en) * 1998-07-21 2004-07-15 Dow Agrosciences Llc Antibody-mediated down-regulation of plant proteins
BR9912745A (en) * 1998-08-04 2001-11-06 Cargill Inc Promoters of plant fatty acid denaturase
GB9914209D0 (en) 1999-06-17 1999-08-18 Danisco Process
EP1908843A1 (en) 1999-08-26 2008-04-09 Calgene LLC Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7157619B1 (en) 1999-08-30 2007-01-02 Monsanto Technology, L.L.C. Plant sterol acyltransferases
US6974898B2 (en) 2000-04-18 2005-12-13 Commonwealth Scientific And Industrial Research Organisation Method of modifying the content of cottonseed oil
US6706950B2 (en) 2000-07-25 2004-03-16 Calgene Llc Nucleic acid sequences encoding β-ketoacyl-ACP synthase and uses thereof
BR0113512A (en) 2000-08-25 2005-05-10 Basf Plant Science Gmbh Plant polynucleotides encoding new prenyl proteases
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
US6897357B2 (en) 2000-09-26 2005-05-24 Regents Of The University Of California Characterization of phenylalanine ammonia-lyase (PAL) gene in wounded lettuce tissue
WO2005003312A2 (en) * 2003-06-27 2005-01-13 Monsanto Technology, Llc Elevation of oil levels in plants
US7820883B2 (en) 2006-03-15 2010-10-26 Dow Agrosciences Llc Resistance to auxinic herbicides
EP2090658A3 (en) 2006-06-13 2009-11-11 Agrinomics, LLC Generation of plants with improved pathogen resistance
WO2008000511A2 (en) * 2006-06-30 2008-01-03 Freie Universität Berlin Pektin methyltransferases and their applications
BR102012008162B1 (en) * 2012-04-09 2018-08-14 Empresa Brasileira De Pesquisa Agropecuária - Embrapa compositions and methods for modifying the expression of genes of interest

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0321201A2 (en) * 1987-12-15 1989-06-21 Gene Shears Pty. Limited Ribozymes
EP0360257A2 (en) * 1988-09-20 1990-03-28 The Board Of Regents For Northern Illinois University RNA catalyst for cleaving specific RNA sequences
EP0416572A1 (en) * 1989-09-07 1991-03-13 Hoechst Schering AgrEvo GmbH RNAs with endoribonucleasic activity against mRNA from ripening genes, their preparation and their use in plants
WO1991013972A1 (en) * 1990-03-16 1991-09-19 Calgene, Inc. Plant desaturases - compositions and uses
WO1991018985A1 (en) * 1990-05-25 1991-12-12 E.I. Du Pont De Nemours And Company Nucleotide sequence of soybean stearoyl-acp desaturase gene
WO1992013090A1 (en) * 1991-01-17 1992-08-06 The General Hospital Corporation Trans-splicing ribozymes
WO1993002197A1 (en) * 1991-07-23 1993-02-04 Nickerson Biocem Limited CALLASE-RELATED DNAs AND THEIR USE IN ARTIFICIAL MALE STERILITY
WO1994000012A1 (en) * 1992-06-29 1994-01-06 Gene Shears Pty. Ltd. Nucleic acids and methods of use thereof for controlling viral pathogens
WO1995006128A2 (en) * 1993-08-25 1995-03-02 Dekalb Genetics Corporation Fertile, transgenic maize plants and methods for their production

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0321201A2 (en) * 1987-12-15 1989-06-21 Gene Shears Pty. Limited Ribozymes
EP0360257A2 (en) * 1988-09-20 1990-03-28 The Board Of Regents For Northern Illinois University RNA catalyst for cleaving specific RNA sequences
EP0416572A1 (en) * 1989-09-07 1991-03-13 Hoechst Schering AgrEvo GmbH RNAs with endoribonucleasic activity against mRNA from ripening genes, their preparation and their use in plants
WO1991013972A1 (en) * 1990-03-16 1991-09-19 Calgene, Inc. Plant desaturases - compositions and uses
WO1991018985A1 (en) * 1990-05-25 1991-12-12 E.I. Du Pont De Nemours And Company Nucleotide sequence of soybean stearoyl-acp desaturase gene
WO1992013090A1 (en) * 1991-01-17 1992-08-06 The General Hospital Corporation Trans-splicing ribozymes
WO1993002197A1 (en) * 1991-07-23 1993-02-04 Nickerson Biocem Limited CALLASE-RELATED DNAs AND THEIR USE IN ARTIFICIAL MALE STERILITY
WO1994000012A1 (en) * 1992-06-29 1994-01-06 Gene Shears Pty. Ltd. Nucleic acids and methods of use thereof for controlling viral pathogens
WO1995006128A2 (en) * 1993-08-25 1995-03-02 Dekalb Genetics Corporation Fertile, transgenic maize plants and methods for their production

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KNUTZON, D.S., ET AL.: "Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 89, 1992, WASHINGTON US, pages 2624 - 2628, XP002022969 *
KUIPERS, A.G.J., ET AL.: "Field evaluation of transgenic potato plants expressing an antisense granule-bound starch synthase gene: increase of the antisense effect during tuber growth", PLANT MOLECULAR BIOLOGY, vol. 26, 1994, pages 1758 - 1773, XP002021172 *
WEGENER, D., ET AL.: "Expression of a reporter gene is reduced by a ribozyme in transgenic plants", MOLECULAR AND GENERAL GENETICS, vol. 245, 1994, pages 465 - 470, XP002022959 *

Also Published As

Publication number Publication date
WO1997010328A2 (en) 1997-03-20
MX9800454A (en) 1998-04-30
EP0842286A2 (en) 1998-05-20
BR9610402A (en) 2000-01-11
CN1196091A (en) 1998-10-14
AU6761796A (en) 1997-04-01
CA2226728A1 (en) 1997-03-20
JPH11509733A (en) 1999-08-31

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