[go: up one dir, main page]

WO1997001647A3 - Sequences d'adn pour identifier des lignees hautement transmissibles de pseudomonas (burkholderia) cepacia - Google Patents

Sequences d'adn pour identifier des lignees hautement transmissibles de pseudomonas (burkholderia) cepacia Download PDF

Info

Publication number
WO1997001647A3
WO1997001647A3 PCT/US1996/011132 US9611132W WO9701647A3 WO 1997001647 A3 WO1997001647 A3 WO 1997001647A3 US 9611132 W US9611132 W US 9611132W WO 9701647 A3 WO9701647 A3 WO 9701647A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
lineages
pseudomonas cepacia
highly transmissible
cepacia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/011132
Other languages
English (en)
Other versions
WO1997001647A2 (fr
Inventor
Richard Goldstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Trustees Of Health & Hospitals Of City Of Bost
Trustees Of Health & Hospitals
Original Assignee
Trustees Of Health & Hospitals Of City Of Bost
Trustees Of Health & Hospitals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trustees Of Health & Hospitals Of City Of Bost, Trustees Of Health & Hospitals filed Critical Trustees Of Health & Hospitals Of City Of Bost
Priority to AU64041/96A priority Critical patent/AU6404196A/en
Publication of WO1997001647A2 publication Critical patent/WO1997001647A2/fr
Publication of WO1997001647A3 publication Critical patent/WO1997001647A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des empreintes et des séquences d'ADN permettant d'identifier des lignées hautement transmissibles de Pseudomonas cepacia. Plus particulièrement, l'invention concerne des motifs de bandes génétiques ou des empreintes d'ADN permettant d'identifier des lignées hautement transmissibles de Pseudomonas cepacia. Ces motifs sont obtenus (a) par analyse des ribotypes, c'est-à-dire la détermination du polymorphisme de fragments de restriction (RFLP) associés à l'opéron d'ARN en copies multiples et (b) par électrophorèse sur gel à champs pulsés pour la résolution de motifs macro-RFLP chromosomiques. L'invention concerne également des séquences spéciales d'amorces oligonucléotidiques et de sondes d'ADN dérivées des variants d'un gène, codant une sous-unité majeure de 17 KDa de la protéine piline (cb1A) de Pseudomonas cepacia associé à la mucoviscidose, pour une utilisation dans des procédés PCR pour la détection de souches hautement transmissibles de Pseudomonas cepacia. L'invention concerne également des procédés et des trousses permettant de diagnostiquer des lignées hautement transmissibles de Pseudomonas cepacia, qui utilisent les empreintes d'ADN, les amorces oligonucléotidiques et les sondes d'ADN en question. Un autre objet de cette invention est de fournir de molécules d'ADN isolées codant une sous-unité principale de 17 KDa de la protéine piline de certaines souches de Pseudomonas cepacia associées à la mucoviscidose, des molécules d'ADN de recombinaison, des hôtes transformés et des procédés de production de cette protéine. L'invention concerne également des anticorps contre la sous-unité majeure de 17 Kda de la protéine piline.
PCT/US1996/011132 1995-06-28 1996-06-28 Sequences d'adn pour identifier des lignees hautement transmissibles de pseudomonas (burkholderia) cepacia Ceased WO1997001647A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU64041/96A AU6404196A (en) 1995-06-28 1996-06-28 Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82895P 1995-06-28 1995-06-28
US60/000,828 1995-06-28

Publications (2)

Publication Number Publication Date
WO1997001647A2 WO1997001647A2 (fr) 1997-01-16
WO1997001647A3 true WO1997001647A3 (fr) 1997-05-01

Family

ID=21693176

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/011132 Ceased WO1997001647A2 (fr) 1995-06-28 1996-06-28 Sequences d'adn pour identifier des lignees hautement transmissibles de pseudomonas (burkholderia) cepacia

Country Status (2)

Country Link
AU (1) AU6404196A (fr)
WO (1) WO1997001647A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6630302B1 (en) * 1997-07-25 2003-10-07 The Trustees Of Boston University Methods and compositions for determining species of bacteria and fungi
AU5612099A (en) * 1998-09-03 2000-03-27 University Of British Columbia, The Method for the identification and speciation of bacteria of the (burkholderia cepacia) complex
RU2458140C1 (ru) * 2011-02-17 2012-08-10 Федеральное государственное учреждение здравоохранения "Волгоградский научно-исследовательский противочумный институт Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека Способ выявления экспрессирующихся генов патогенных буркхольдерий методом дифференциального дисплея

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BINGEN E H ET AL: "USE OF RIBOTYPING IN EPIDEMIOLOGICAL SURVEILLANCE OF NOSOCOMIAL OUTBREAKS", CLINICAL MICROBIOLOGY REVIEWS, vol. 7, no. 3, 1 July 1994 (1994-07-01), pages 311 - 327, XP000604155 *
DASEN S E ET AL: "CHARACTERIZATION OF PCR-RIBOTYPING FOR BURKHOLDERIA (PSEUDOMONAS) CEPACIA", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 32, no. 10, October 1994 (1994-10-01), pages 2422 - 2424, XP000604176 *
GOLDSTEIN R ET AL: "STRUCTURALLY VARIANT CLASSES OF PILUS APPENDAGE FIBERS COEXPRESSED FROM BURKHOLDERIA (PSEUDOMONAS) CEPACIA", JOURNAL OF BACTERIOLOGY, vol. 177, no. 4, 1 February 1995 (1995-02-01), pages 1039 - 1052, XP000604151 *
O'CALLAGHAN E M ET AL: "DEVELOPMENT OF A PCR PROBE TEST FOR IDENTIFYING PSEUDOMONAS AERUGINOSA AND PSEUDOMONAS (BURKHOLDERIA) CEPACIA", JOURNAL OF CLINICAL PATHOLOGY, vol. 47, no. 3, March 1994 (1994-03-01), pages 222 - 226, XP000603979 *
RYLEY H C ET AL: "TYPING OF PSEUDOMONAS CEPACIA ISOLATES FROM WELSH CF PATIENTS BY PCR OF RNA RIBOSOMAL GENES", INTERNATIONAL CONGRESS SERIES, 21 May 1993 (1993-05-21), pages 95 - 99, XP000604136 *
SAJJAN U S ET AL: "CABLE (CBL) TYPE II PILI OF CYSTIC FIBROSIS-ASSOCIATED BURKHOLDERIA (PSEUDOMONAS) CEPACIA: NUCLEOTIDE SEQUENCE OF THE CBLA MAJOR SUBUNIT PILIN GENE AND NOVEL MORPHOLOGY OF THE ASSEMBLED APPENDAGE FIBERS", JOURNAL OF BACTERIOLOGY, vol. 177, no. 4, February 1995 (1995-02-01), pages 1030 - 1038, XP000603985 *
SUN L ET AL: "THE EMERGENCE OF A HIGHLY TRANSMISSIBLE LINEAGE OF CBL PSEUDOMONAS (BURKHOLDERIA) CEPACIA CAUSING CF CENTRE EPIDEMICS IN NORTH AMERICA AND BRITAIN", NATURE MEDICINE, vol. 1, no. 7, July 1995 (1995-07-01), pages 661 - 666, XP000605148 *

Also Published As

Publication number Publication date
WO1997001647A2 (fr) 1997-01-16
AU6404196A (en) 1997-01-30

Similar Documents

Publication Publication Date Title
Kohlstaedt et al. Reverse transcriptase of human immunodeficiency virus can use either human tRNA (3Lys) or Escherichia coli tRNA (2Gln) as a primer in an in vitro primer-utilization assay.
Pansegrau et al. Concerted action of three distinct domains in the DNA cleaving-joining reaction catalyzed by relaxase (TraI) of conjugative plasmid RP4.
Vincent et al. Epigenetic regulation of the human mucin gene MUC4 in epithelial cancer cell lines involves both DNA methylation and histone modifications mediated by DNA methyltransferases and histone deacetylases
EP0788307A4 (fr) Modulation par les oligonucleotides de la proteine associee a la resistance multiples aux anticancereux
WO2000031235A3 (fr) Sequences nucleotidiques et proteiques de gene nogo et procedes reposant sur ces sequences
Travers RNA polymerase and T4 development
EP1837406A3 (fr) Séquençage d'acide nucléique par surveillance Raman par incorporation de précurseurs au cours de la réplication moléculaire
FR2685346B1 (fr) Procede de preparation d'arn double-brin, et ses applications.
WO1997018334A3 (fr) Sondes d'acides nucleiques complementaires des acides nucleiques du virus du papillome humain, methodes et preparations associees
WO2003048378A3 (fr) Methodes de clivage selectif d'acides nucleiques duplex a adn dans un melange d'acides nucleiques complexes, et compositions de nucleases utilisees dans lesdites methodes
BR9809433A (pt) Moléculas de dna enzimáticas
Pope et al. Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G: A substrates
Holmes et al. Structural requirements for tRNA methylation. Action of Escherichia coli tRNA (guanosine-1) methyltransferase on tRNA (1Leu) structural variants.
ATE333516T1 (de) Transkriptionsbasierende vervielfältigung von dopplesträngigen dns-zielmolekülen
Krey et al. Interactions of the antibiotic, distamycin A, with native DNA and with synthetic duplex polydeoxyribonucleotides
AU5741399A (en) Two-colour differential display, used as a method for detecting regulated genes
WO1997001647A3 (fr) Sequences d'adn pour identifier des lignees hautement transmissibles de pseudomonas (burkholderia) cepacia
Raskine et al. Novel plasmid-encoded class C β-lactamase (MOX-2) in Klebsiella pneumoniae from Greece
Barthelemy et al. In vivo transcription of bacteriophage phi 29 DNA: transcription termination
Thompson et al. Interactions of calf thymus DNA polymerase α with primer/templates
WO2004097003A3 (fr) Amplification de sequences d'acide nucleique par plusieurs amorces
PT80909A (fr) Sonde et procede pour la detection de micro-organismes determi-nes notamment des legionella dans les milieux les contenant
Vernel-Pauillac et al. Genotyping as a tool for antibiotic resistance surveillance of Neisseria gonorrhoeae in New Caledonia: evidence of a novel genotype associated with reduced penicillin susceptibility
Ali et al. Action of human endonucleases III and VIII upon DNA-containing tandem dihydrouracil
WO2002083081A3 (fr) Compositions se rapportant au cancer de la prostate, procedes et trousses fondes sur des plates-formes proteomiques de jeu ordonne de macrodepots d'adn

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IL IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG UZ VN AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IL IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TR TT UA UG UZ VN AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA