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WO1997001647A3 - Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia - Google Patents

Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia Download PDF

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Publication number
WO1997001647A3
WO1997001647A3 PCT/US1996/011132 US9611132W WO9701647A3 WO 1997001647 A3 WO1997001647 A3 WO 1997001647A3 US 9611132 W US9611132 W US 9611132W WO 9701647 A3 WO9701647 A3 WO 9701647A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
lineages
pseudomonas cepacia
highly transmissible
cepacia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/011132
Other languages
French (fr)
Other versions
WO1997001647A2 (en
Inventor
Richard Goldstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Trustees Of Health & Hospitals Of City Of Bost
Trustees Of Health & Hospitals
Original Assignee
Trustees Of Health & Hospitals Of City Of Bost
Trustees Of Health & Hospitals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trustees Of Health & Hospitals Of City Of Bost, Trustees Of Health & Hospitals filed Critical Trustees Of Health & Hospitals Of City Of Bost
Priority to AU64041/96A priority Critical patent/AU6404196A/en
Publication of WO1997001647A2 publication Critical patent/WO1997001647A2/en
Publication of WO1997001647A3 publication Critical patent/WO1997001647A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides DNA based fingerprints and DNA sequences for indentifying highly transmissible lineages of Pseudomonas cepacia. More specifically, the present invention provides genetic band patterns or DNA based fingerprints for identifying highly transmissible lineages of Pseudomonas cepacia produced by: (a) ribotyping analyses, i.e., the determination of restriction fragment length polymorphisms (RFLPs) associated with the multicopy RNA operon (rrn); and (b) pulsed field gel electrophoresis (PFGE)-based resolution of chromosomal macro-RFLP patterns. Also provided are unique primer oligonucleotide sequences and DNA probes derived from variants of a gene, encoding a 17-KDa major subunit pilin protein (cb1A) of a cystic fibrosis-associated Pseudomonas cepacia. The invention also discloses methods and diagnostic kits for identifying highly transmissible lineages of Pseudomonas cepacia that utilize the above DNA based fingerprints, oligonucleotide primers and DNA probes. Another object of this invention is to provide isolated DNA molecules encoding a 17-KDa major subunit pilin protein of certain cystic fibrosis-associated Pseudomonas cepacia strains, as well as recombinant DNA molecules, transformed hosts and methods for the production of that protein. Also contemplated are antibodies to the 17-KDa major subunit pilin protein.
PCT/US1996/011132 1995-06-28 1996-06-28 Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia Ceased WO1997001647A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU64041/96A AU6404196A (en) 1995-06-28 1996-06-28 Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82895P 1995-06-28 1995-06-28
US60/000,828 1995-06-28

Publications (2)

Publication Number Publication Date
WO1997001647A2 WO1997001647A2 (en) 1997-01-16
WO1997001647A3 true WO1997001647A3 (en) 1997-05-01

Family

ID=21693176

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/011132 Ceased WO1997001647A2 (en) 1995-06-28 1996-06-28 Dna sequences for identifying highly transmissible lineages of pseudomonas (burkholderia) cepacia

Country Status (2)

Country Link
AU (1) AU6404196A (en)
WO (1) WO1997001647A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6630302B1 (en) * 1997-07-25 2003-10-07 The Trustees Of Boston University Methods and compositions for determining species of bacteria and fungi
AU5612099A (en) * 1998-09-03 2000-03-27 University Of British Columbia, The Method for the identification and speciation of bacteria of the (burkholderia cepacia) complex
RU2458140C1 (en) * 2011-02-17 2012-08-10 Федеральное государственное учреждение здравоохранения "Волгоградский научно-исследовательский противочумный институт Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека Method for detecting expressing genes of pathogenic burcholderia by differential display

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BINGEN E H ET AL: "USE OF RIBOTYPING IN EPIDEMIOLOGICAL SURVEILLANCE OF NOSOCOMIAL OUTBREAKS", CLINICAL MICROBIOLOGY REVIEWS, vol. 7, no. 3, 1 July 1994 (1994-07-01), pages 311 - 327, XP000604155 *
DASEN S E ET AL: "CHARACTERIZATION OF PCR-RIBOTYPING FOR BURKHOLDERIA (PSEUDOMONAS) CEPACIA", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 32, no. 10, October 1994 (1994-10-01), pages 2422 - 2424, XP000604176 *
GOLDSTEIN R ET AL: "STRUCTURALLY VARIANT CLASSES OF PILUS APPENDAGE FIBERS COEXPRESSED FROM BURKHOLDERIA (PSEUDOMONAS) CEPACIA", JOURNAL OF BACTERIOLOGY, vol. 177, no. 4, 1 February 1995 (1995-02-01), pages 1039 - 1052, XP000604151 *
O'CALLAGHAN E M ET AL: "DEVELOPMENT OF A PCR PROBE TEST FOR IDENTIFYING PSEUDOMONAS AERUGINOSA AND PSEUDOMONAS (BURKHOLDERIA) CEPACIA", JOURNAL OF CLINICAL PATHOLOGY, vol. 47, no. 3, March 1994 (1994-03-01), pages 222 - 226, XP000603979 *
RYLEY H C ET AL: "TYPING OF PSEUDOMONAS CEPACIA ISOLATES FROM WELSH CF PATIENTS BY PCR OF RNA RIBOSOMAL GENES", INTERNATIONAL CONGRESS SERIES, 21 May 1993 (1993-05-21), pages 95 - 99, XP000604136 *
SAJJAN U S ET AL: "CABLE (CBL) TYPE II PILI OF CYSTIC FIBROSIS-ASSOCIATED BURKHOLDERIA (PSEUDOMONAS) CEPACIA: NUCLEOTIDE SEQUENCE OF THE CBLA MAJOR SUBUNIT PILIN GENE AND NOVEL MORPHOLOGY OF THE ASSEMBLED APPENDAGE FIBERS", JOURNAL OF BACTERIOLOGY, vol. 177, no. 4, February 1995 (1995-02-01), pages 1030 - 1038, XP000603985 *
SUN L ET AL: "THE EMERGENCE OF A HIGHLY TRANSMISSIBLE LINEAGE OF CBL PSEUDOMONAS (BURKHOLDERIA) CEPACIA CAUSING CF CENTRE EPIDEMICS IN NORTH AMERICA AND BRITAIN", NATURE MEDICINE, vol. 1, no. 7, July 1995 (1995-07-01), pages 661 - 666, XP000605148 *

Also Published As

Publication number Publication date
WO1997001647A2 (en) 1997-01-16
AU6404196A (en) 1997-01-30

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