WO1997048491B1 - Method for achieving chemical, in particular biochemical reactions and pipette tip with reaction vessel and with optional additional receiving component for said pipette tip - Google Patents

Abstract

The invention proposes a method for achieving chemical, in particular biochemical reactions in a reaction liquid (168) using a pipette tip (114) of a pipetting device, by means of which the reaction liquid (168) is picked up by a suction opening (126) in the pipette tip (114) and transferred to a reaction chamber (149) in a reaction liquid receiving unit (112). At least one part or part of the component of the reaction liquid (168) is transferred to the reaction chamber (149) using the pipette tip (114). To seal the reaction chamber (149), an opening in the reaction chamber is sealed by means of the pipette tip (114), whereby the suction opening (126) of the pipette tip lies inside the reaction chamber (149). The reaction takes place inside the reaction chamber (149).

Claims

 33 CHANGED CLAIMS [received at the International Bureau on 17 December 1997 (17.12.97); original claim 1 changed; all other claims unchanged (1 page)] Method for carrying out chemical, in particular biochemical, reactions in a reaction liquid (168; 268) using a pipetting tip (114; 214) of a pipetting device by means of the reaction liquid (168; 268) is receivable through a suction opening (126; 226) of the pipetting tip (114; 214) for subsequent delivery into a reaction space (149; 249) of a reaction liquid receiving unit (112; 212; 212a) a portion of the reaction liquid (168; 268) with the pipetting tip (114; 214) into the reaction space (149; 249), that at the end of the reaction space (149; 249) a reaction space opening (156; 280) by means of the pipette tip (114, 142, 214) of the pipetting device, wherein the suction opening (126; 226) of the pipette tip lies within the reaction space (149; 249) and that the reaction is allowed to take place in the reaction space (149; 249), characterized in that at the end of the reaction space (149; 249), the reaction space opening (156; 280) is completely hermetically sealed by means of the pipetting tip (114, 142; 214) having a pipetting piston (134). 34 Annex Declaration according to Art. 19.1 new claim 1 For the upcoming international preliminary 3-fold examination is handed over a new claim 1, which replaces the original claim 1. The new claim 1 is based on the original claim 1, as well as on the description page 5, first paragraph, line 1, page 13, first paragraph, lines 4 and 5, page 1 6, third paragraph, lines 18 - 20 and page 17, second paragraph and is compared with the nearest coming WO 83/01 91 2 A delineated. WO 83/0191 2 A discloses a test tube, which is provided in the region of its reaction space opening with an elastic rubber stopper which serves to hermetically seal the test tube when the pneumatically actuated pipette tip is pulled out of the rubber stopper. In this case, an additional cover may be provided (Fig. 4 to 6) to prevent that when inserting the pipette tip in the rubber stopper on the outside of the rubber stopper stripped sample liquid can be discharged in the further handling to the environment. If the pipette tip is inserted in the rubber plug, air exchange with the environment is possible in principle. By contrast, in the subject matter of the claim 1 submitted hereby, a completely airtight closure of the reaction space is achieved by the pipetting tip inserted into the reaction space opening and provided with a pipetting piston. The pipette tip therefore does not have to be pulled out of the reaction space opening for complete sealing of the reaction space and, after the reaction has ended, if a withdrawal of the reaction liquid is desired, it is reinserted into the rubber stopper. (In case of increased risk of contamination would have to use a fresh pipette for this reaction liquid withdrawal.) The invention thus facilitates handling at a significantly reduced risk of contamination. JP 07 080331 A discloses a device in which a pipetting tip which is open on both sides is provided on its outside with a cover part. The pneumatically actuated pipette tip, together with the cover part, serves to close a container, with the possibility of fluid intake into the pipetting tip.