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WO1996039101B1 - Compositions and methods for naturally secreted extracellular matrix - Google Patents

Compositions and methods for naturally secreted extracellular matrix

Info

Publication number
WO1996039101B1
WO1996039101B1 PCT/US1996/009482 US9609482W WO9639101B1 WO 1996039101 B1 WO1996039101 B1 WO 1996039101B1 US 9609482 W US9609482 W US 9609482W WO 9639101 B1 WO9639101 B1 WO 9639101B1
Authority
WO
WIPO (PCT)
Prior art keywords
extracellular matrix
cells
naturally secreted
framework
composition according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/009482
Other languages
French (fr)
Other versions
WO1996039101A1 (en
Filing date
Publication date
Priority claimed from US08/470,101 external-priority patent/US5830708A/en
Application filed filed Critical
Priority to JP50179097A priority Critical patent/JP3709487B2/en
Priority to NZ311313A priority patent/NZ311313A/en
Priority to EP96921369A priority patent/EP0967934B1/en
Priority to DE69634454T priority patent/DE69634454T2/en
Priority to AU62609/96A priority patent/AU708090B2/en
Priority to AT96921369T priority patent/ATE290350T1/en
Publication of WO1996039101A1 publication Critical patent/WO1996039101A1/en
Publication of WO1996039101B1 publication Critical patent/WO1996039101B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

The present invention discloses compositions containing naturally secreted human extracellular matricies, the methods of making those matricies, and the method of using those matricies. The extracellular matrix is obtained by growing cells into a three-dimensional support structure so that the cells secrete an extracellular matrix. Next, the cells are killed and removed from the support structure leaving only the extracellular matrix on the support structure. The extracellular matrix can then be removed from the support, combined with a suitable pharmacologically acceptable carrier and injected into a tissue defect area in the skin of a patient.

Claims

AMENDED CLAIMS [received by the International Bureau on 27 November 1996 (27.11.96); original claims 1-29 replaced by amended claims 1-15 (3 pages)]
1. A method for the production of a naturally secreted extracellular matrix coated three-dimensional framework comprising the steps of:
(a) culturing cells on a three-dimensional framework under conditions favorable for cellular growth for a pre-determined time period such that an extracellular matrix is secreted onto the inoculated framework creating a coated framework; (b) killing the cells and
(c) removing the cells and cellular debris.
2. A method according to claim 1 wherein the three-dimensional framework is a material selected from the group consisting of polyamide, polyester, polystyrene, polypropylene, polyacrylates, polyvinyl compounds,
polycarbonate, polytetrafluorethylene, thermanox,
nitrocellulose, cotton, polyglycolie acid, catgut suture material, cellulose, gelatin, chitosan, hyaluronic acid and dextran,
3. A method according to claim 1 or 2, wherein the three-dimensional framework has pore spaces of about 150μm to about 220μm.
4. The method according to any one of claims 1 to 3, wherein the extracellular matrix is secreted by tissue specific cells selected from the group consisting of
fibroblasts, osteoblasts, odontoblasts, chondrocytes, epithelial cells, smoothst-muscle cells, retinal cells, endothelial cells, stromal cells or combinations thereof.
5. A method for the production of a naturally secreted extracellular matrix comprising carrying out steps (a), (b) and (c) according to any one of claims 1 to 4 and then (d) collecting the extracellular matrix deposited on the coated framework.
6. A composition comprising a three-dimensional framework coated with a naturally secreted extracellular matrix composed of human proteins.
7. A composition according to claim 6, wherein the framework is made from a material selected from the group consisting of polyamide, polyester, polystyrene,
polypropylene, polyacrylates, polyvinyl compounds,
polycarbonate, polytetrafluorethylene, thermanox,
nitrocellulose, cotton, polyglycolic acid, catgut sutures, cellulose, gelatin, chitosan, hyaluronic acid and dextran.
8. A composition according to claim 6 or 7, wherein the three-dimensional framework has pore spaces of about 150μm to about 220μm.
9. A composition according to any one of claims 6 to 8, wherein the naturally secreted extracellular matrix is secreted by tissue specific ceils selected from the group consisting of fibroblasts, oεteoblasts, odontoblasts, chondrocytes, epithelial cells, smooth muscle cells, retinal cells, endothelial cells, stromal cells or combinations thereof.
10. An injectable naturally secreted extracellular matrix composition for the treatment of tissue defects comprising a human naturally secreted extracellular matrix and a pharmaceutically acceptable carrier.
11. A composition according to claim 10, wherein the naturally secreted extracellular matrix is produced by a method according to claim 5.
12. A composition according to claim 11 wherein naturally secreted extracellular matrices secreted by
different tissue or cell types are mixed between said step (d) and step (e), consisting in processing the collected extracellular matrix with a pharmaceutically acceptable carrier, such that ratios of collagen types I-V, respective to each other, are adjusted.
13. A method for the repair of tissue defects comprising injecting at a site of a tissue defect a
composition according to any one of claims 10-12.
14. A method according to claim 13 wherein the naturally secreted extracellular matrix is produced by a method according to claim 5.
15. The use of a composition according to any one of claims 10-12 for the repair of tissue defects.
PCT/US1996/009482 1995-06-06 1996-06-06 Compositions and methods for naturally secreted extracellular matrix Ceased WO1996039101A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP50179097A JP3709487B2 (en) 1995-06-06 1996-06-06 Compositions and methods for naturally secreted extracellular matrix
NZ311313A NZ311313A (en) 1995-06-06 1996-06-06 Composition and method for making compositions for treatment of wrinkles and scars using human extracellular matrix
EP96921369A EP0967934B1 (en) 1995-06-06 1996-06-06 Compositions and methods for naturally secreted extracellular matrix
DE69634454T DE69634454T2 (en) 1995-06-06 1996-06-06 Compositions and methods relating to a naturally secreted matrix
AU62609/96A AU708090B2 (en) 1995-06-06 1996-06-06 Compositions and methods for naturally secreted extracellular matrix
AT96921369T ATE290350T1 (en) 1995-06-06 1996-06-06 COMPOSITIONS AND METHODS RELATING TO A NATURALLY SECRETED MATRIX

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/470,101 US5830708A (en) 1995-06-06 1995-06-06 Methods for production of a naturally secreted extracellular matrix
US470,101 1995-06-06

Publications (2)

Publication Number Publication Date
WO1996039101A1 WO1996039101A1 (en) 1996-12-12
WO1996039101B1 true WO1996039101B1 (en) 1997-01-09

Family

ID=23866272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/009482 Ceased WO1996039101A1 (en) 1995-06-06 1996-06-06 Compositions and methods for naturally secreted extracellular matrix

Country Status (11)

Country Link
US (1) US5830708A (en)
EP (1) EP0967934B1 (en)
JP (1) JP3709487B2 (en)
KR (1) KR19990022216A (en)
AT (1) ATE290350T1 (en)
AU (1) AU708090B2 (en)
CA (1) CA2223892A1 (en)
DE (1) DE69634454T2 (en)
ES (1) ES2238076T3 (en)
NZ (1) NZ311313A (en)
WO (1) WO1996039101A1 (en)

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