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WO1996031781B1 - System for releasing and isolating nucleic acids - Google Patents

System for releasing and isolating nucleic acids

Info

Publication number
WO1996031781B1
WO1996031781B1 PCT/EP1996/001368 EP9601368W WO9631781B1 WO 1996031781 B1 WO1996031781 B1 WO 1996031781B1 EP 9601368 W EP9601368 W EP 9601368W WO 9631781 B1 WO9631781 B1 WO 9631781B1
Authority
WO
WIPO (PCT)
Prior art keywords
sample processing
unit
nucleic acids
receiving unit
releasing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1996/001368
Other languages
German (de)
French (fr)
Other versions
WO1996031781A1 (en
Filing date
Publication date
Priority claimed from DE19512368A external-priority patent/DE19512368A1/en
Application filed filed Critical
Priority to EP96909143A priority Critical patent/EP0819255B1/en
Priority to JP52995196A priority patent/JP3773264B2/en
Priority to DE59610764T priority patent/DE59610764D1/en
Priority to US08/930,247 priority patent/US6117398A/en
Priority to AT96909143T priority patent/ATE251754T1/en
Publication of WO1996031781A1 publication Critical patent/WO1996031781A1/en
Publication of WO1996031781B1 publication Critical patent/WO1996031781B1/en
Anticipated expiration legal-status Critical
Priority to US09/558,038 priority patent/US6919175B1/en
Ceased legal-status Critical Current

Links

Abstract

A process for releasing and isolating nucleic acids from biological compartments of a sample makes use of a device which is suitable for accepting one or more sample processing containers, thermostatically controlling and agitating the sample processing containers and magnetically separating magnetic particles. The isolation of nucleic acids is substantially simplified thereby.

Claims

36 36 GEÄNDERTE ANSPRÜCHECHANGED CLAIMS [beim Internationalen Büro am 30. September 1996 (30.09.96) eingegangen; ursprünglicher Anspruch 5 geändert; alle weiteren Ansprüche unverändert (1 Seite)][received at the International Bureau on 30 September 1996 (30.09.96); original claim 5 changed; all other claims unchanged (1 page)] System zur Freisetzung und Isolierung mit Magnetpartikeln enthaltend die KomponentenSystem for release and isolation with magnetic particles containing the components - eine Aufnahmeeinheit (10, 100) zur Aufnahme eines oder mehrerer Probenbearbei¬ tungsgefäße (A),a receiving unit (10, 100) for receiving one or more sample processing vessels (A), - eine Thermostatoreinheit (20) zur Thermostatisierung der Probenbearbeitungsge- faße (A) und der darin enthaltenen Flüssigkeiten,a thermostatic unit (20) for thermostating the sample processing tray (A) and the liquids contained therein, - einen Schuttler(30) zum Schuttein der Probenbearbeitungsgefaße (A),a shedder (30) for debris from the sample processing vessels (A), - einen Abscheider (40) zur magnetischen Abscheidung der Magnetpartikel an einer Wand jedes Probenbearbeitungsgefaßes (A),a separator (40) for magnetically depositing the magnetic particles on a wall of each sample processing vessel (A), in aufeinander abgestimmter Kopplungin coordinated coupling 6. System gemäß Anspruch 5, dadurch gekennzeichnet, daß es zusatzlich eine Pumpen¬ einheit (50) zur Entfernung von Flüssigkeit aus dem Probenbearbeitungsgefaß (A) enthalt6. System according to claim 5, characterized in that it additionally contains a Pumpen¬ unit (50) for the removal of liquid from the Probenbearbeitungsgefaß (A) 7 System gemäß Anspruch 5 oder 6, dadurch gekennzeichnet, daß der Abscheider (40) und die Aufnahmeeinheit (10) relativ zueinander beweglich gelagert sind7 System according to claim 5 or 6, characterized in that the separator (40) and the receiving unit (10) are mounted movable relative to each other 8. System gemäß Anspruch 5, dadurch gekennzeichnet, daß die Probenbearbeitungsge¬ faß (A) eine untere Auslaßoffnung (Al l ) aufweisen, die mit einer Saugvorrichtung verbunden ist oder verbunden werden kann8. System according to claim 5, characterized in that the Probenbearbeitungsge¬ barrel (A) having a lower Auslaßoffnung (Al l), which is connected to a suction device or can be connected 9 System gemäß Anspruch 5, dadurch gekennzeichnet, daß es eine Vielzahl von Probenbearbeitungsgefaßen (A) enthaltA system according to claim 5, characterized in that it includes a plurality of sample processing vessels (A) 10 System gemäß Anspruch 5, dadurch gekennzeichnet, daß die Aufnahmeeinheit (10, 100) durch einen Exzenterantrieb innerhalb einer Ebene bewegt wird, die im wesent¬ lichen senkrecht zur Achse des bzw der Probenbearbeitungsgefaße ist10. System according to claim 5, characterized in that the receiving unit (10, 100) is moved by an eccentric drive within a plane which is substantially perpendicular to the axis of the sample processing vessel (s) 1 1 System gemäß Anspruch 5, dadurch gekennzeichnet, daß die Thermostatoreinheit eine Widerstandsheizung sowie ein Peltierelement beinhaltet 1 1 system according to claim 5, characterized in that the thermostatic unit includes a resistance heater and a Peltier element
PCT/EP1996/001368 1995-04-01 1996-03-28 System for releasing and isolating nucleic acids Ceased WO1996031781A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP96909143A EP0819255B1 (en) 1995-04-01 1996-03-28 System for releasing and isolating nucleic acids
JP52995196A JP3773264B2 (en) 1995-04-01 1996-03-28 Systems used for nucleic acid release and isolation
DE59610764T DE59610764D1 (en) 1995-04-01 1996-03-28 SYSTEM FOR THE RELEASE AND ISOLATION OF NUCLEIC ACIDS
US08/930,247 US6117398A (en) 1995-04-01 1996-03-28 System for releasing and isolating nucleic acids
AT96909143T ATE251754T1 (en) 1995-04-01 1996-03-28 NUCLEIC ACID RELEASE AND ISOLATION SYSTEM
US09/558,038 US6919175B1 (en) 1995-04-01 2000-04-26 System for releasing and isolating nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19512368A DE19512368A1 (en) 1995-04-01 1995-04-01 Nucleic acid release and isolation system
DE19512368.9 1995-04-01

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US08930247 A-371-Of-International 1996-03-28
US09/558,038 Division US6919175B1 (en) 1995-04-01 2000-04-26 System for releasing and isolating nucleic acids

Publications (2)

Publication Number Publication Date
WO1996031781A1 WO1996031781A1 (en) 1996-10-10
WO1996031781B1 true WO1996031781B1 (en) 1996-11-28

Family

ID=7758614

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1996/001368 Ceased WO1996031781A1 (en) 1995-04-01 1996-03-28 System for releasing and isolating nucleic acids

Country Status (7)

Country Link
US (1) US6117398A (en)
EP (1) EP0819255B1 (en)
JP (1) JP3773264B2 (en)
AT (1) ATE251754T1 (en)
DE (2) DE19512368A1 (en)
ES (1) ES2206565T3 (en)
WO (1) WO1996031781A1 (en)

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