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WO1991018982A1 - Recepteurs de l'interleukine-1 de type ii - Google Patents

Recepteurs de l'interleukine-1 de type ii Download PDF

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WO1991018982A1
WO1991018982A1 PCT/US1991/003498 US9103498W WO9118982A1 WO 1991018982 A1 WO1991018982 A1 WO 1991018982A1 US 9103498 W US9103498 W US 9103498W WO 9118982 A1 WO9118982 A1 WO 9118982A1
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type
protein
leu
ser
thr
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John E. Sims
David J. Cosman
Stephen D. Lupton
Bruce A. Mosley
Steven K. Dower
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Immunex Corp
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Immunex Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • the present invention relates generally to cytokine receptors, and more specifically, to Interleukin- 1 receptors.
  • Interleukin- l ⁇ (IL-l ⁇ ) and Interleukin- l ⁇ and (IL-l ⁇ ) are distantly related polypeptide hormones which play a central role in the regulation of immune and inflammatory responses. These two proteins act on a variety of cell types and have multiple biological activities.
  • the diversity of biological activity ascribed to IL-l ⁇ and IL- l ⁇ is mediated by specific plasma membrane receptors which bind both IL-l ⁇ and IL-l ⁇ . Due to the wide range of biological activities mediated by IL-l ⁇ and IL-l ⁇ it was originally believed that the IL-1 receptors should be highly conserved in a variety of species and expressed on a large variety of cells.
  • the IL-1 receptor on murine and human T cells has been identified by cDNA expression cloning and N-terminal sequence analysis as an integral membrane glycoprotein that binds IL-l ⁇ and IL-l ⁇ and has a molecular weight of 80,000 kDa (Sims et al., Science 247:585, 1988; Sims et al., Proc. Natl. Acad. Sci. USA 86:8946, 1989).
  • compositions are needed. Such compositions are theoretically available via purification of receptors expressed by cultured cells, or by cloning and expression of genes encoding the receptors. Prior to the present invention, however, several obstacles prevented these goals from being achieved.
  • I ' attempts may be required to define a probe having the requisite specificity to identify a hybridizing sequence in a cDNA library.
  • direct expression cloning techniques avoid the need for repetitive screening using different probes of unknown specificity and have been useful in cloning other receptors (e.g., type I IL-IR), they are not sufficiently sensitive to be suitable for using in identifying type II IL-IR clones from cDNA libraries derived from cells expressing low numbers of type II IL-IR.
  • the present invention provides purified and homogeneous type II IL-IR proteins and isolated DNA sequences encoding type II IL-IR proteins, in particular, human type II IL-IR, or analogs thereof.
  • DNA sequences are selected from the group consisting of (a) cDNA clones having a nucleotide sequence derived from the coding region of a native type II IL-IR gene, such as clone 75; (b) DNA sequences capable of hybridization to the cDNA clones of (a) under moderately stringent conditions and which encode biologically active IL-IR molecules; and (c) DNA sequences which are degenerate as a result of the genetic code to the DNA sequences defined in (a) and (b) and which encode biologically active IL-IR molecules.
  • the present invention also provides recombinant expression vectors comprising the DNA sequences defined above, recombinant type II IL-IR molecules produced using the recombinant expression vectors, and processes for producing the recombinant type II IL-IR molecules utilizing the expression vectors.
  • the present invention also provides substantially purified and homogeneous proteins and protein compositions comprising type II BL-1R.
  • the present invention also provides compositions for use in therapy, diagnosis, assay of type II IL-IR, or in raising antibodies to type II IL-IR, comprising effective quantities of soluble native or recombinant receptor proteins prepared according to the foregoing processes.
  • FIG. 1 is a schematic diagram of the expression plasmid pDC406. cDNA molecules inserted at the Sal site are transcribed and translated using regulatory elements
  • pDC406 contains origins of replication derived from
  • FIG. 2 is a schematic diagram of the human and murine type II IL-1 receptors and the various human and murine clones used to determine the sequences. Thin lines represent untranslated regions, while the coding region is depicted by a box. The sections encoding the signal peptide are filled in; the transmembrane regions are cross-hatched; and the cytoplasmic portions are stippled. Potential N-linked glycosylation sites are marked by inverted triangles. The predicted immunoglobulin-like disulfide bonds are also indicated by dashes connecting two sulfide molecules (S S).
  • FIG. 3 compares the amino acid sequences of the human and murine type II IL-1 receptors (as deduced from the cDNA clones) with the amino acid sequences of the human and murine type I IL-1 receptors (Sims et al., Proc. Natl. Acad. Sci. USA 86:8946, 1989; Sims et al., Science 242:585, 1988) and the amino acid sequences of the ST2 cellular gene (Tominaga, FEBS Lett.258:301, 1989) and the B15R open reading frame of vaccinia virus (Smith and Chan, J. Gen. Virology 72:511, 1991). Numbering begins with the initiating methionine.
  • the predicted position of the signal peptide cleavage in each sequences was determined according to the method described by von Heijne, Nucl. Acids. Res. 24:4683, - 1986, and is indicated by a gap between the putative signal peptide and the main body of the protein.
  • the predicated transmembrane and cytoplasmic regions for the type ⁇ IL-1 receptors are shown on the bottom line, and are separated from one another by a gap. Residues conserved in all four IL-1 receptor sequences are presented in white on a black background.
  • Residues conserved in type II receptors that are also found in one of the other sequences are shaded; residues conserved in type I IL-1 receptors that are found in one of the other sequences are boxed. Cysteine residues involved in forming the disulfide bonds characteristic of the immunoglobulin fold are marked with solid dots, while the extra two pairs of cysteines found in the type I IL-1 receptor and in some of the other sequences are indicated by stars. The approximate boundaries of domains 1, 2 and 3 are indicated above the lines.
  • the predicted signal peptide cleavage in the type II IL-1 receptors follow Alal3, resulting in an unusually short signal peptide and an N-terminal extension of 12 (human) or 23 (mouse) amino acids beyond the point corresponding to the mature N-terminus of the human or mouse type I IL-1 receptor.
  • Other less favored but still acceptable sites of cleavage in the murine type II BL-1 receptor are after Thrl5 or Prol7.
  • This sequence alignment was made by hand and does not represent an objectively optimized alignment of the sequences.
  • the nucleotide and amino acid sequences of the full length and soluble human and murine type II IL-1 receptor cDNAs are also set forth in the Sequence Listing herein.
  • FIG. 4 shows an autoradiograph of an SDS/PAGE gel with crosslinked IL-1 receptors.
  • Cells expressing ⁇ L-1 receptors were cross-linked to 125 I-IL-1 in the absence or presence of the cognate unlabeled IL-1 competitor, extracted, electrophoresed and autoradiographed as described in Example 6.
  • Recombinant receptors were expressed transiently in CV1/EBNA cells.
  • the cell lines used for cross-linking to natural receptors were KB (ATCC CCL 1717) (for human type I D -1R) , CB23 (for human type II IL-IR), EL4 (ATCC TIB 39) (for murine type I IL-IR), and 70Z/3 (ATCC TIB 158) (for murine type II IL-IR).
  • Type II Interleukin- 1 receptor and “type II IL-IR” refer to proteins which are capable of binding Interleukin-1 (IL-1) molecules and, in their native configuration as mammalian plasma membrane proteins, play a role in transducing the signal provided by IL-1 to a cell.
  • the mature full-length human type II IL-IR is a glycoprotein having an apparent molecular weight of approximately 60-68 kDa.
  • Specific examples of type II IL- IR proteins are shown in SEQ ID NO:l and SEQ ID NO: 12. As used herein, the above terms include analogs or subunits of native type II IL-IR proteins with IL-1 -binding or signal transducing activity.
  • type II IL-IR refers generically to mammalian type II IL-IR, which includes, but is not limited to, human, murine, and bovine type II IL-IR.
  • type II IL-IR means all forms of type II IL-IR, including mutants and analogs .which possess type II IL-IR biological activity.
  • Interleukin- 1 Receptor or "IL-IR” refers collectively to type I IL-1 receptor and type II IL-1 receptor.
  • Soluble type II IL-IR refers to proteins, or substantially equivalent analogs, which are substantially similar to all or part of the extracellular region of a native type II IL-IR, and are secreted by the cell but retain the ability to bind IL-1 or inhibit IL-1 signal transduction activity via cell surface bound IL-IR proteins. Soluble type II IL-IR proteins may also include part of the transmembrane region, provided that the soluble type II IL-IR protein is capable of being secreted from the cell. Specific examples of soluble type II IL-IR proteins include proteins having the sequence of amino acids 1-330 or amino acids 1-333 of SEQ ID NO:l and amino acids 1- 342 and amino acids 1-345 of SEQ ID NO: 12.
  • Inhibition of IL-1 signal transduction activity can be determined using primary cells or cells lines which express an endogenous IL-IR and which are biologically responsive to IL-1 or which, when transfected with recombinant IL-IR DNAs, are biologically responsive to IL-1.
  • the cells are then contacted with IL-1 and the resulting metabolic effects examined. If an effect results which is attributable to the action of the ligand, then the recombinant receptor has signal transduction activity.
  • Exemplary procedures for determining whether a polypeptide has signal transduction activity are disclosed by Idzerda et al., J. Exp. Med. 272:861 (1990); Curtis et al., Proc. Natl. Acad. Sci.
  • the term "isolated” or “purified”, as used in the context of this specification to define the purity of type II IL-IR protein or protein compositions, means that the protein or protein composition is substantially free of other proteins of natural or endogenous origin and contains less than about 1% by mass of protein contaminants residual of production processes. Such compositions, however, can contain other proteins added as stabilizers, carriers, excipients or co-therapeutics.
  • Type II IL-IR is "isolated” if it is detectable as a single protein band in a polyacrylamide gel by silver staining.
  • substantially similar when used to define either amino acid or nucleic acid sequences, means that a particular subject sequence, for example, a mutant sequence, varies from a reference sequence by one or more substitutions, deletions, or additions, the net effect of which is to retain biological activity of the type II IL-IR protein as may be determined, for example, in a type II IL-IR binding assays, such as is described in Example 5 below.
  • nucleic acid subunits and analogs are "substantially similar" to the specific DNA sequences disclosed herein if: (a) the DNA sequence is derived from the coding region of SEQ-ID NO: 1 or SEQ ID NO: 12; (b) the DNA sequence is capable of hybridization to DNA sequences of (a) under moderately stringent conditions (25% formamide, 42° C, 2xSSC) or alternatively under more stringent conditions (50% formamide, 50°C, 2xSSC or 50% formamide, 42° C, 2xSSC) and which encode biologically active IL-IR molecules; or DNA sequences which are degenerate as a result of the genetic code to the DNA sequences defined in (a) or (b) and which encode biologically active IL-IR molecules.
  • moderately stringent conditions (25% formamide, 42° C, 2xSSC) or alternatively under more stringent conditions (50% formamide, 50°C, 2xSSC or 50% formamide, 42° C, 2xSSC) and which encode biologically active IL-
  • Recombinant means that a protein is derived from recombinant (e.g., microbial or mammalian) expression systems.
  • Microbial refers to recombinant proteins made in bacterial or fungal (e.g., yeast) expression systems.
  • recombinant microbial defines a protein essentially free of native endogenous substances and unaccompanied by associated native glycosylation. Protein expressed in most bacterial cultures, e.g., E. coli, will be free of glycan; protein expressed in yeast may have a glycosylation pattern different from that expressed in mammalian cells.
  • Bioly active as used throughout the specification as a characteristic of type II IL-IR, means either that a particular molecule shares sufficient amino acid sequence similarity with SEQ ID NO:2 or SEQ ID NO: 13 to be capable of binding detectable quantities of IL-1, preferably at least 0.01 nmoles IL-1 per nanomole type II IL-IR, or, in the alternative, shares sufficient amino acid sequence similarity to be capable of transmitting an IL-1 stimulus to a cell, for example, as a component of a hybrid receptor construct.
  • biologically active type II IL-IR within the scope of the present invention is capable of binding greater than 0.1 nanomoles IL-1 per nanomole receptor, and most preferably, greater than 0.5 nanomoles IL-1 per nanomole receptor.
  • DNA sequence refers to a DNA polymer, in the form of a separate fragment or as a component of a larger DNA construct, which has been derived from DNA isolated at least once in substantially pure form, i.e., free of contaminating endogenous materials and in a quantity or concentration enabling identification, manipulation, and recovery of the sequence and its component nucleotide sequences by standard biochemical methods, for example, using a cloning vector.
  • sequences are preferably provided in the form of an open reading frame uninterrupted by internal nontranslated sequences, or introns, which are typically present in eukaryotic genes.
  • genomic DNA containing the relevant sequences could also be used.
  • Sequences of non-translated DNA may be present 5' or 3' from the open reading frame, where the same do not interfere with manipulation or expression of the coding regions.
  • Nucleotide sequence refers to a heteropolymer of deoxyribonucleotides. DNA sequences encoding the proteins provided by this invention are assembled from cDNA fragments and short oligonucleotide linkers, or from a series of oligonucleotides, to provide a synthetic gene which is capable of being expressed in a recombinant transcriptional unit.
  • Recombinant expression vector refers to a plasmid comprising a transcriptional unit comprising an assembly of (1) a genetic element or elements having a regulatory role in gene expression, for example, promoters or enhancers, (2) a structural or coding sequence which is transcribed into mRNA and translated into protein, and (3) appropriate transcription and translation initiation and termination sequences.
  • Structural elements intended for use in yeast expression systems preferably include a leader sequence enabling extracellular secretion of translated protein by a host cell.
  • recombinant protein may include an N-terminal methionine residue. This residue may optionally be subsequently cleaved from the expressed recombinant protein to provide a final product.
  • Recombinant microbial expression system means a substantially homogeneous monoculture of suitable host microorganisms, for example, bacteria such as E. coli or yeast such as S. cerevisiae, which have stably integrated a recombinant transcriptional unit into chromosomal DNA or carry the recombinant transcriptional unit as a component of a resident plasmid. Generally, cells constituting the system are the progeny of a single ancestral transformant. Recombinant expression systems as defined herein will express heterologous protein upon induction of the regulatory elements linked to the DNA sequence or synthetic gene to be expressed. Type II IL-IR Proteins and Analogs
  • the present invention provides isolated recombinant mammalian type II IL-IR polypeptides.
  • the type II IL-IR proteins of the present invention include, by way of example, primate, human, murine, canine, feline, bovine, ovine, equine, caprine and porcine type ⁇ IL-IR.
  • Type ⁇ IL-IR can be obtained by cross species hybridization, using a single stranded cDNA derived from the human or murine type II IL-IR DNA sequence, for example, human clone 75, as a hybridization probe to isolate type II IL-IR cDNAs from ma ⁇ -malian cDNA libraries.
  • mammalian type II IL- IR is presumably encoded by multi-exon genes.
  • Alternative mRNA constructs which can be attributed to different mRNA splicing events following transcription, and which share large regions of identity or similarity with the cDNAs claimed herein, are considered to be within the scope of the present invention.
  • DNA sequences which encode IL-IR-II can be isolated from the following cells and tissues: B lymphoblastoid lines (such as CB23, CB33, Raji, RPMI1788, ARH77), resting and especially activated peripheral blood T cells, monocytes, the monocytic cell line THP1, neutrophils, bone marrow, placenta, endothelial cells, keratinocytes (especially activated), and HepG2 cells.
  • B lymphoblastoid lines such as CB23, CB33, Raji, RPMI1788, ARH77
  • Type II IL-IR within the scope of the invention also include various structural forms of the primary protein which retain biological activity. Due to the presence of ionizable amino and carboxyl groups, for example, a type II IL-IR protein may be in the form of acidic or basic salts, or may be in neutral form. Individual amino acid residues may also be modified by oxidation or reduction.
  • the primary amino acid structure may be modified by forming covalent or aggregative conjugates with other chemical moieties, such as glycosyl groups, lipids, phosphate, acetyl groups and the like, or by creating amino acid sequence mutants.
  • Covalent derivatives are prepared by linking particular functional groups to type II IL-IR amino acid side chains or at the N- or C-termini.
  • Other derivatives of type II IL-IR within the scope of this invention include covalent or aggregative conjugates of type II IL-IR or its fragments with other proteins or polypeptides, such as by synthesis in recombinant culture as N-terminal or C-terminal fusions.
  • the conjugated peptide may be a a signal (or leader) polypeptide sequence at the N-terminal region of the protein which co- translationally or post-translationally directs transfer of the protein from its site of synthesis to its site of function inside or outside of the cell membrane or wall (e.g., the yeast ⁇ -factor leader).
  • Type II IL-IR protein fusions can comprise peptides added to facilitate purification or identification of type II IL- 1R (e.g., poly-His).
  • the amino acid sequence of type II IL- IR can also be linked to the peptide Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (DYKDDDDK) (Hopp et al., Bio/Technology 6:1204,1988.)
  • the latter sequence is highly antigenic and provides an epitope reversibly bound by a specific monoclonal antibody, enabling rapid assay and facile purification of expressed recombinant protein.
  • This sequence is also specifically cleaved by bovine mucosal enterokinase at the residue immediately following the Asp-Lys pairing. Fusion proteins capped with this peptide may also be resistant to intracellular degradation in E. coli.
  • Type II IL-IR derivatives may also be used as immunogens, reagents in receptor- based immunoassays, or as binding agents for affinity purification procedures of IL-1 or other binding ligands.
  • Type II IL-IR derivatives may also be obtained by cross-linking agents, such as M-maleimidobenzoyl succinimide ester and N-hydroxysuccinimide, at cysteine and lysine residues.
  • Type II IL-IR proteins may also be covalently bound through reactive side groups to various insoluble substrates, such as cyanogen bromide-activated, bisoxirane-activated, carbonyldiimidazole-activated or tosyl-activated agarose structures, or by adsorbing to polyolefin surfaces (with or without glutaraldehyde cross-linking). Once bound to a substrate, type II IL-IR may be used to selectively bind (for purposes of assay or purification) anti-type II IL-IR antibodies or IL-1.
  • the present invention also includes type II IL-IR with or without associated native- pattern glycosylation.
  • Type II IL-IR expressed in yeast or mammalian expression systems e.g., COS-7 cells, may be similar or slightly different in molecular weight and glycosylation pattern than the native molecules, depending upon the expression system.
  • Expression of type II IL-IR DNAs in bacteria such as E. coli provides non-glycosylated molecules.
  • Functional mutant analogs of mammalian type II IL-IR having inactivated N- glycosylation sites can be produced by oligonucleotide synthesis and ligation or by site- specific mutagenesis techniques. These analog proteins can be produced in a homogeneous, reduced-carbohydrate form in good yield using yeast expression systems.
  • N-glycosylation sites in eukaryotic proteins are characterized by the amino acid triplet Asn- Ai-Z, where Ai is any amino acid except Pro, and Z is Ser or Thr.
  • asparagine provides a side chain amino group for covalent attachment of carbohydrate.
  • Examples of N-glycosylation sites in human type II IL-IR are amino acids 66-68, 72-74, 112-114, 219-221, and 277-279 in SEQ ID NO:l. Such sites can be eliminated by substituting another amino acid for Asn or for residue Z, deleting Asn or Z, or inserting a non-Z amino acid between Ai and Z, or an amino acid other than Asn between Asn and Al.
  • Type II IL-IR derivatives may also be obtained by mutations of type ⁇ IL-IR or its subunits.
  • a type II IL-IR mutant as referred to herein, is a polypeptide homologous to type II IL-IR but which has an amino acid sequence different from native type II IL-IR because of a deletion, insertion or substitution.
  • Bioequivalent analogs of type II IL-IR proteins may be constructed by, for example, making various substitutions of residues or sequences or deleting terminal or internal residues or sequences not needed for biological activity. For example, cysteine residues can be deleted or replaced with other amino acids to prevent formation of . unnecessary or incorrect intramolecular disulfide bridges upon renaturation.
  • mutagenesis involves modification of adjacent dibasic amino acid residues to enhance expression in yeast systems in which KEX2 protease activity is present. Generally, substitutions should be made conservatively; i.e., the most preferred substitute amino acids are those having phy siochemical characteristics resembling those of the residue to be replaced. Similarly, when a deletion or insertion strategy is adopted, the potential effect of the deletion or insertion on biological activity should be considered.
  • Substantially similar polypeptide sequences, as defined above, generally comprise a like number of amino acids sequences, although C-terminal truncations for the purpose of constructing soluble type II IL-IRs will contain fewer amino acid sequences.
  • deletions and substitutions will preferably result in homologous or conservatively substituted sequences, meaning that a given residue is replaced by a biologically similar residue.
  • conservative substitutions include substitution of one aliphatic residue for another, such as He, Val, Leu, or Ala for one another, or substitutions of one polar residue for another, such as between Lys and Arg; Glu and Asp; or Gin and Asn.
  • Other such conservative substitutions for example, substitutions of entire regions having similar hydrophobicity characteristics, are well known.
  • particular amino acid differences between human, murine and other mammalian type II B -lRs is suggestive of additional conservative substitutions that may be made without altering the essential biological characteristics of type II IL-IR.
  • Subunits of type II IL-IR may be constructed by deleting terminal or internal residues or sequences.
  • the present invention contemplates, for example, C terminal deletions which result in soluble type II EL-IR constructs corresponding to all or part of the extracellular region of type II IL-IR.
  • the resulting protein preferably retains its ability to bind IL-1.
  • Particularly preferred sequences include those in which the transmembrane region and intracellular domain of type II IL-IR are deleted or substituted with hydrophilic residues to facilitate secretion of the receptor into the cell culture medium.
  • Soluble type II IL-IR proteins may also include part of the transmembrane region, provided that the soluble type II IL-IR protein is capable of being secreted from the cell.
  • soluble human type II IL-IR may comprise the sequence of amino acids 1-333 or amino acids 1-330 of SEQ ID NO:l and amino acids 1-345 and amino acids 1-342 of SEQ ID NO: 12.
  • soluble type II IL-IR proteins may be derived by deleting the C- terminal region of a type II IL-IR within the extracellular region which are not necessary for IL-1 binding.
  • C-terminal deletions may be made to proteins having the sequence of SEQ ID NO:l and SEQ ID NO:12 following amino acids 313 and 325, respectively.
  • These amino acids are cysteines which are believed to be necessary to maintain the tertiary structure of the type II IL-IR molecule and permit binding of the type II IL-IR molecule to IL-1.
  • Soluble type ⁇ IL-IR constructs are constructed by deleting the 3'-terminal region of a DNA encoding the type II IL-IR and then inserting and expressing the DNA in appropriate expression vectors. Exemplary methods of constructing such soluble proteins are described in Examples 2 and 4. The resulting soluble type II IL-IR proteins are then assayed for the ability to bind B -1, as described in Example 5. Both the DNA sequences encoding such soluble type II IL-IRs and the biologically active soluble type II IL-IR proteins resulting from such constructions are contemplated to be within the scope of the present invention.
  • Mutations in nucleotide sequences constructed for expression of analog type II IL- IR must, of course, preserve the reading frame phase of the coding sequences and preferably will not create complementary regions that could hybridize to produce secondary mRNA structures such as loops or hairpins which would adversely affect translation of the receptor mRNA.
  • a mutation site may be predetermined, it is not necessary that the nature of the mutation per se be predetermined. For example, in order to select for optimum characteristics of mutants at a given site, random mutagenesis may be conducted at the target codon and the expressed type II IL-IR mutants screened for the desired activity.
  • nucleotide sequence which encodes type II IL-IR will be expressed in the final product, for example, nucleotide substitutions may be made to enhance expression, primarily to avoid secondary structure loops in the transcribed mRNA (see EPA 75.444A, incorporated herein by reference), or to provide codons that are more readily translated by the selected host, e.g., the well-known E. coli preference codons for E. coli expression.
  • Mutations can be introduced at particular loci by synthesizing oligonucleotides containing a mutant sequence, flanked by restriction sites enabling ligation to fragments of the native sequence. Following ligation, the resulting reconstructed sequence encodes an analog having the desired amino acid insertion, substitution, or deletion.
  • oligonucleotide-directed site-specific mutagenesis procedures can be employed to provide an altered gene having particular codons altered according to the substitution, deletion, or insertion required.
  • Exemplary methods of making the alterations set forth above are disclosed by Walder et al. (Gene 42:133, 1986); Bauer et al. (Gene 37:73, 1985); Craik (BioTechniques, January 1985, 12-19); Smith et al. (Genetic Engineering: Principles and Methods, Plenum Press, 1981); and U.S. Patent Nos. 4,518,584 and 4,737,462 disclose suitable techniques, and are incorporated by reference herein.
  • polyvalent forms possess multiple type ⁇ IL- IR binding sites for IL-1 ligand.
  • a bivalent soluble type II IL-IR may consist of two tandem repeats of the extracellular region of type II IL-IR, separated by a linker region.
  • Alternate polyvalent forms may also be constructed, for example, by chemically coupling type II IL-IR to any clinically acceptable carrier molecule, a polymer selected from the group consisting of Ficoll, polyethylene glycol or dextran using conventional coupling techniques.
  • type II B -IR may be chemically coupled to biotin, and the biotin-type II IL-IR conjugate then allowed to bind to avidin, resulting in tetravalent avidin/biotin/type ⁇ IL-IR molecules.
  • Type ⁇ IL-IR may also be covalently coupled to dinitrophenol (DNP) or trinitrophenol (TNP) and the resulting conjugate precipitated with anti-DNP or anti-TNP-IgM, to form decameric conjugates with a valency of 10 for type II IL-IR binding sites.
  • DNP dinitrophenol
  • TNP trinitrophenol
  • a recombinant chimeric antibody molecule may also be produced having type II IL- IR sequences substituted for the variable domains of either or both of the immunoglubulin molecule heavy and light chains and having unmodified constant region domains.
  • chimeric type II IL-lR/IgGi may be produced from two chimeric genes — a type II IL-lR/human K light chain chimera (type II IL-1R/C K ) and a type II B -lR/human ⁇ l heavy chain chimera (type II IL-1R/C ⁇ . ⁇ ). Following transcription and translation of the two chimeric genes, the gene products assemble into a single chimeric antibody molecule having type II IL-IR displayed bivalently. Such polyvalent forms of type II IL-IR may have enhanced binding affinity for IL-1 ligand. Additional details relating to the construction of such chimeric antibody molecules are disclosed in WO 89/09622 and EP 315062.
  • the present invention provides recombinant expression vectors to amplify or express DNA encoding type II BL-IR.
  • Recombinant expression vectors are replicable DNA constructs which have synthetic or cDNA-derived DNA fragments encoding mammalian type II IL-IR or bioequivalent analogs operably linked to suitable transcriptional or translational regulatory elements derived from mammalian, microbial, viral or insect genes.
  • a transcriptional unit generally comprises an assembly of (1) a genetic element or elements having a regulatory role in gene expression, for example, transcriptional promoters or enhancers, (2) a structural or coding sequence which is transcribed into mRNA and translated into protein, and (3) appropriate transcription and translation initiation and termination sequences, as described in detail below.
  • Such regulatory elements may include an operator sequence to control transcription, a sequence encoding suitable mRNA ribosomal binding sites.
  • the ability to replicate in a host, usually conferred by an origin of replication, and a selection gene to facilitate recognition of transformants may additionally be incorporated.
  • DNA regions are operably linked when they are functionally related to each other. For example, DNA for a signal peptide (secretory leader) is operably linked to DNA for a polypeptide if it is expressed as a precursor which participates in the secretion of the polypeptide; a promoter is operably linked to a coding sequence if it controls the transcription of the sequence; or a ribosome binding site is operably linked to a coding sequence if it is positioned so as to permit translation.
  • signal peptide secretory leader
  • a promoter is operably linked to a coding sequence if it controls the transcription of the sequence
  • a ribosome binding site is operably linked to a coding sequence if it is positioned so
  • operably linked means contiguous and, in the case of secretory leaders, contiguous and in reading frame.
  • Structural elements intended for use in yeast expression systems preferably include a leader sequence enabling extracellular secretion of translated protein by a host cell.
  • recombinant protein may include an N-terminal methionine residue. This residue may optionally be subsequently cleaved from the expressed recombinant protein to provide a final product
  • DNA sequences encoding mammalian type II IL-IRs which are to be expressed in a microorganism will preferably contain no introns that could prematurely terminate transcription of DNA into mRNA; however, premature termination of transcription may be desirable, for example, where it would result in mutants having advantageous C-terminal truncations, for example, deletion of a transmembrane region to yield a soluble receptor not bound to the cell membrane. Due to code degeneracy, there can be considerable variation in nucleotide sequences encoding the same amino acid sequence. Other embodiments include sequences capable of hybridizing to clone 75 under moderately stringent conditions (50° C, 2x SSC) and other sequences hybridizing or degenerate to those which encode biologically active type II BL-IR polypeptides.
  • moderately stringent conditions 50° C, 2x SSC
  • Recombinant type II IL-IR DNA is expressed or amplified in a recombinant expression system comprising a substantially homogeneous monoculture of suitable host microorganisms, for example, bacteria such as E. coli or yeast such as S. cerevisiae, which have stably integrated (by transformation or transfection) a recombinant transcriptional unit into chromosomal DNA or carry the recombinant transcriptional unit as a component of a resident plasmid.
  • suitable host microorganisms for example, bacteria such as E. coli or yeast such as S. cerevisiae, which have stably integrated (by transformation or transfection) a recombinant transcriptional unit into chromosomal DNA or carry the recombinant transcriptional unit as a component of a resident plasmid.
  • suitable host microorganisms for example, bacteria such as E. coli or yeast such as S. cerevisiae, which have stably integrated (by transformation or transfection) a
  • Transformed host cells are cells which have been transformed or transfected with type II IL-IR vectors constructed using recombinant ' DNA techniques.
  • Transformed host cells ordinarily express type II IL-IR, but host cells transformed for purposes of cloning or amplifying type II IL-IR DNA do not need to express type II IL-IR.
  • Expressed type II JL- 1R will be deposited in the cell membrane or secreted into the culture supernatant, depending on the type II IL-IR DNA selected.
  • Suitable host cells for expression of mammalian type II IL-IR include prokaryotes, yeast or higher eukaryotic cells under the control of appropriate promoters. Prokaryotes include gram negative or gram positive organisms, for example E.
  • coli or bacilli Higher eukaryotic cells include established cell lines of mammalian origin as described below. Cell-free translation systems could also be employed to produce mammalian type ⁇ IL-IR using RNAs derived from the DNA constructs of the present invention. Appropriate cloning and expression vectors for use with bacterial, fungal, yeast, and mammalian cellular hosts are described by Pouwels et al. (Cloning Vectors: A Laboratory Manual, Elsevier, New York, 1985), the relevant disclosure of which is hereby incorporated by reference.
  • Prokaryotic expression hosts may be used for expression of type II IL-IR that do not require extensive proteolytic and disulfide processing.
  • Prokaryotic expression vectors generally comprise one or more phenotypic selectable markers, for example a gene encoding proteins conferring antibiotic resistance or supplying an autotrophic requirement, and an origin of replication recognized by the host to ensure amplification within the host.
  • Suitable prokaryotic hosts for transformation include E. coli, Bacillus subtilis, Salmonella typkimurium, and various species within the genera Pseudomonas, Streptomyces, and Staphyolococcus, although others may also be employed as a matter of choice.
  • Useful expression vectors for bacterial use can comprise a selectable marker and bacterial origin of replication derived from commercially available plasmids comprising genetic elements of the well known cloning vector pBR322 (ATCC 37017).
  • Such commercial vectors include, for example, pKK223-3 (Pharmacia Fine Chemicals, Uppsala, Sweden) and pGEMl (Promega Biotec, Madison, WI, USA).
  • pBR322 "backbone" sections are combined with an appropriate promoter and the structural sequence to be expressed.
  • E. coli is typically transformed using derivatives of pBR322, a plasmid derived from an E. coli species (Bolivar et al., Gene 2:95, 1977).
  • pBR322 contains genes for ampicillin and tetracycline resistance and thus provides simple means for identifying transformed cells.
  • Promoters commonly used in recombinant microbial expression vectors include the ⁇ -lactamase (penicillinase) and lactose promoter system (Chang et al., Nature 275:615, 1978; and Goeddel et al., Nature 282:544, 1979), the tryptophan (tip) promoter system (Goeddel et al., Nucl. Acids Res. 8:4057, 1980; and EPA 36,776) and tac promoter (Maniatis, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, p. 412, 1982).
  • a particularly useful bacterial expression system employs the phage ⁇ PL promoter and cI857ts thermolabile repressor.
  • Plasmid vectors available from the American Type Culture Collection which incorporate derivatives of the ⁇ PL promoter include plasmid pHUB2, resident in E. coli strain JMB9 (ATCC 37092) and pPLc28, resident in E. coli RR1 (ATCC 53082).
  • Recombinant type II EL-1R proteins may also be expressed in yeast hosts, preferably from the Saccharomyces species, such as S. cerevisiae. Yeast of other genera, such as Pichia or Kluyveromyces may also be employed. Yeast vectors will generally contain an origin of replication from the 2 ⁇ yeast plasmid or an autonomously replicating sequence (ARS), promoter, DNA encoding type ⁇ IL-IR, sequences for polyadenylation and transcription termination and a selection gene. Preferably, yeast vectors will include an origin of replication and selectable marker permitting transformation of both yeast and E. coli, e.g., the ampicillin resistance gene of E. coli and S.
  • ARS autonomously replicating sequence
  • TRP1 or URA3 gene which provides a selection marker for a mutant strain of yeast lacking the ability to grow in tryptophan, and a promoter derived from a highly expressed yeast gene to induce transcription of a structural sequence downstream.
  • the presence of the TRP1 or URA3 lesion in the yeast host cell genome then provides an effective environment for detecting transformation by growth in the absence of tryptophan or uracil.
  • Suitable promoter sequences in yeast vectors include the promoters for metallothionein, 3-phosphoglycerate kinase (Hitzeman et al., /. Biol. Chem. 255:2073, 1980) or other glycolytic enzymes (Hess et al., J. Adv. Enzyme Reg. 7:149, 1968; and Holland et al., Biochem.
  • enolase such as enolase, glyceraldehyde-3- ⁇ hosphate dehydrogenase, hexokinase, pyruvate decarboxylase, phosphofructokinase, glucose-6- phosphate isomerase, 3-phosphoglycerate mutase, pyruvate kinase, triosephosphate isomerase, phosphoglucose isomerase, and glucokinase.
  • Suitable vectors and promoters for use in yeast expression are further described in R. Hitzeman et al., EPA 73,657.
  • Preferred yeast vectors can be assembled using DNA sequences from pUC18 for selection and replication in E. coli (Amp r gene and origin of replication) and yeast DNA sequences including a glucose-repressible ADH2 promoter and ⁇ -factor secretion leader.
  • the ADH2 promoter has been described by Russell et al. (/. Biol. Chem.258:2674, 1982) and Beier et al. (Nature 300:124,. 1982).
  • the yeast ⁇ -factor leader which directs secretion of heterologous proteins, can be inserted between the promoter and the structural gene to be expressed. See, e.g., Kurjan et al., Cell 30:933, 1982; and Bitter et al., Proc. Natl. Acad. Sci. USA 82:5330, 1984.
  • the leader sequence may be modified to contain, near its 3' end, one or more useful restriction sites to facilitate fusion of the leader sequence to foreign genes.
  • Suitable yeast transformation protocols are known to those of skill in the art; an exemplary technique is described by Hinnen et al., Proc. Natl. Acad. Sci. USA 75:1929, 1978, selecting for Trp + transformants in a selective medium consisting of 0.67% yeast nitrogen base, 0.5% casamino acids, 2% glucose, 10 ⁇ g/ml adenine and 20 ⁇ g/ml uracil or URA+ transformants in medium consisting of 0.67% YNB, with amino acids and bases as described by Sherman et al., Laboratory Course Manual for Methods in Yeast Genetics, . Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1986.
  • Host strains transformed by vectors comprising the ADH2 promoter may be grown for expression in a rich medium consisting of 1% yeast extract, 2% peptone, and 1% or 4% glucose supplemented with 80 ⁇ g/ml adenine and 80 ⁇ g/ml uracil. Derepression of the ADH2 promoter occurs upon exhaustion of medium glucose. Crude yeast supernatants are harvested by filtration and held at 4 * C prior to further purification.
  • mammalian or insect cell culture systems are also advantageously employed to express recombinant protein.
  • Expression of recombinant proteins in mammalian cells is particularly preferred because such proteins are generally correctly folded, appropriately modified and completely functional.
  • suitable mammalian host cell lines include the COS-7 lines of monkey kidney cells, described by Gluzman (Cell 23:175, 1981), and other cell lines capable of expressing an appropriate vector including, for example, L cells, C127, 3T3, Chinese hamster ovary (CHO), HeLa and BHK cell lines.
  • Mammalian expression vectors may comprise nontranscribed elements such as an origin of replication, a suitable promoter and enhancer linked to the gene to be expressed, and other 5' or 3' flanking nontranscribed sequences, and 5' or 3' nontranslated sequences, such as necessary ribosome binding sites, a polyadenylation site, splice donor and acceptor sites, and transcriptional termination sequences.
  • nontranscribed elements such as an origin of replication, a suitable promoter and enhancer linked to the gene to be expressed, and other 5' or 3' flanking nontranscribed sequences, and 5' or 3' nontranslated sequences, such as necessary ribosome binding sites, a polyadenylation site, splice donor and acceptor sites, and transcriptional termination sequences.
  • the transcriptional and translational control sequences in expressio vectors to be used in transforming vertebrate cells may be provided by viral sources.
  • viral sources for example, commonly used promoters and enhancers are derived from Polyoma, Adenovirus 2, Simian Virus 40 (SV40), and human cytomegalovirus.
  • DNA sequences derived from the S V40 viral genome for example, SV40 origin, early and late promoter, enhancer, splice, and polyadenylation sites may be used to provide the other genetic elements required for expression of a heterologous DNA sequence.
  • the early and late promoters are particularly useful because both are obtained easily from the virus as a fragment which also contains the SV40 viral origin of replication (Fiers et al., Nature 273:113, 1978).
  • Smaller or larger S V40 fragments may also be used, provided the approximately 250 bp sequence extending from the Hind 3 site toward the Bgll site located in the viral origin of replication is included.
  • mammalian genomic type II IL-IR promoter, control and/or signal sequences may be utilized, provided such control sequences are compatible with the host cell chosen. Additional details regarding the use of a mammalian high expression vector to produce a recombinant mammalian type II IL-IR are provided in Examples 2 below. Exemplary vectors can be constructed as disclosed by Okayama and Berg (Mol. Cell. Biol. 3:280, 1983).
  • a useful system for stable high level expression of mammalian receptor cDNAs in C127 murine mammary epithelial cells can be constructed substantially as described by Cosman et al. (Mol. Immunol. 23:935, 1986).
  • recombinant expression vectors comprising type II IL-IR cDNAs are stably integrated into a host cell's DNA. Elevated levels of expression product is achieved by selecting for cell lines having amplified numbers of vector DNA.
  • Cell lines having amplified numbers of vector DNA are selected, for example, by transforming a host cell with a vector comprising a DNA sequence which encodes an enzyme which is inhibited by a known drug.
  • the vector may also comprise a DNA sequence which encodes a desired protein.
  • the host cell may be co- transformed with a second vector which comprises the DNA sequence which encodes the desired protein. The transformed or co-transformed host cells are then cultured in increasing concentrations of the known drug, thereby selecting for drug-resistant cells.
  • Such drug-resistant cells survive in increased concentrations of the toxic drug by over ⁇ production of the enzyme which is inhibited by the drug, frequently as a result of amplification of the gene encoding the enzyme.
  • drug resistance is caused by an increase in the copy number of the vector DNA encoding the inhibitable enzyme, there is a concomitant co-amplification of the vector DNA encoding the desired protein (e.g., type II IL-IR) in the host cell's DNA.
  • a preferred system for such co-amplification uses the gene for dihydrofolate reductase (DHFR), which can be inhibited by the 'drug methotrexate (MTX).
  • DHFR dihydrofolate reductase
  • MTX 'drug methotrexate
  • a host cell which lacks an active gene encoding DHFR is either transformed with a vector which comprises DNA sequence encoding DHFR and a desired protein, or is co-transformed with a vector comprising a DNA sequence encoding DHFR and a vector comprising a DNA sequence encoding the desired protein.
  • the transformed or co-transformed host cells are cultured in media containing increasing levels of MTX, and those cells lines which survive are selected.
  • a particularly preferred co-amplification system uses the gene for glutamine synthetase (GS), which is responsible for the synthesis of glutamine from glutamate and ammonia using the hydrolysis of ATP to ADP and phosphate to drive the reaction.
  • GS is subject to inhibition by a variety of inhibitors, for example methionine sulphoximine (MSX).
  • MSX methionine sulphoximine
  • type II IL-IR can be expressed in high concentrations by co-amplifying cells transformed with a vector comprising the DNA sequence for GS and a desired protein, or co-transformed with a vector comprising a DNA sequence encoding GS and a vector comprising a DNA sequence encoding the desired protein, culturing the host cells in media containing increasing levels of MSX and selecting for surviving cells.
  • the GS co- amplification system, appropriate recombinant expression vectors and cells lines, are described in the following PCT applications: WO 87/04462, WO 89/01036,
  • Recombinant proteins are preferably expressed by co-amplification of DHFR or GS in a mammalian host cell, such as Chinese Hamster Ovary (CHO) cells, or alternatively in a murine myeloma cell line, such as SP2/0-Agl4 or NS0 or a rat myeloma cell line, such as YB2 3.0-Ag20, disclosed in PCT applications WO/89/10404 and WO 86/05807.
  • a mammalian host cell such as Chinese Hamster Ovary (CHO) cells
  • a murine myeloma cell line such as SP2/0-Agl4 or NS0
  • a rat myeloma cell line such as YB2 3.0-Ag20
  • a preferred eukaryotic vector for expression of type II IL-IR DNA is disclosed below in Example 2.
  • This vector referred to as pDC406, was derived from the mammalian high expression vector pDC201 and contains regulatory sequences from S V40, HIV andEBV.
  • Purified mammalian type II IL-IRs or analogs are prepared by culturing suitable host/vector systems to express the recombinant translation products of the DNAs of the present invention, which are then purified from culture media or cell extracts.
  • supernatants from systems which secrete recombinant soluble type II IL-IR protein into culture media can be first concentrated using a commercially available protein concentration filter, for example, an Amicon or Millipore Pellicon ultrafiltration unit. Following the concentration step, the concentrate can be applied to a suitable purification matrix.
  • a suitable affinity matrix can comprise an EL- 1 or lectin or antibody molecule bound to a suitable support.
  • an anion exchange resin can be employed, for example, a matrix or substrate having pendant diethylaminoethyl (DEAE) groups.
  • the matrices can be acrylamide, agarose, dextran, cellulose or other types commonly employed in protein purification.
  • a cation exchange step can be employed. Suitable cation exchangers include various insoluble matrices comprising sulfopropyl or carboxymethyl groups. Sulfopropyl groups are preferred.
  • RP- HPLC reversed-phase high performance liquid chromatography
  • hydrophobic RP-HPLC media e.g., silica gel having pendant methyl or other aliphatic groups
  • Recombinant protein produced in bacterial culture is usually isolated by initial extraction from cell pellets, followed by one or more concentration, salting-out, aqueous ion exchange or size exclusion chromatography steps. Finally, high performance liquid chromatography (HPLC) can be employed for final purification steps.
  • Microbial cells employed in expression of recombinant mammalian type II IL-IR can be disrupted by any convenient method, including freeze-thaw cycling, sonication, mechanical disruption, or use of cell lysing agents.
  • Fermentation of yeast which express soluble mammalian type II IL-IR as a secreted protein greatly simplifies purification.
  • Secreted recombinant protein resulting from a large- scale fermentation can be purified by methods analogous to those disclosed by Urdal et al. (/. Chromatog. 296:171, 1984).
  • This reference describes two sequential, reversed-phase HPLC steps for purification of recombinant human GM-CSF on a preparative HPLC column.
  • Human type II IL-IR synthesized in recombinant culture is characterized by the presence of non-human cell components, including proteins, in amounts and of a character which depend upon the purification steps taken to recover human type ⁇ IL-IR from the culture. These components ordinarily will be of yeast, prokaryotic or non-human higher eukaryotic origin and preferably are present in innocuous contaminant quantities, on the order of less than about 1 percent by weight. Further, recombinant cell culture enables the production of type II IL-IR free of proteins which may be normally associated with type II IL-IR as it is found in nature in its species of origin, e.g. in cells, cell exudates or body fluids.
  • the present invention provides methods of using therapeutic compositions comprising an effective amount of soluble type II IL-IR proteins and a suitable diluent and carrier, and methods for suppressing 1 IL-1 -dependent immune responses in humans comprising administering an effective amount of soluble type II IL-IR protein.
  • purified soluble type II EL-1R protein is administered to a patient, preferably a human, for treatment in a manner appropriate to the indication.
  • soluble type II IL-IR protein compositions can be administered by bolus injection, continuous infusion, sustained release from implants, or other suitable technique.
  • a soluble type ⁇ IL-IR therapeutic agent will be administered in the form of a composition comprising purified protein in conjunction with physiologically acceptable carriers, excipients or diluents. Such carriers will be nontoxic to recipients at the dosages and concentrations employed.
  • compositions entails combining the type II IL-IR with buffers, antioxidants such as ascorbic acid, low molecular weight (less than about 10 residues) polypeptides, proteins, amino acids, carbohydrates including glucose, sucrose or dextrins, chelating agents such as EDTA, glutathione and other stabilizers and excipients.
  • antioxidants such as ascorbic acid
  • chelating agents such as EDTA, glutathione and other stabilizers
  • excipients Neutral buffered saline or saline mixed with conspecific serum albumin are exemplary appropriate diluents.
  • product is formulated as a lyophilizate using appropriate excipient solutions (e.g., sucrose) as diluents.
  • shuIL-lR dosages of from about 1 ng/kg/day to about 10 mg/kg/day, and more preferably from about 500 ⁇ g/kg/day to about 5 mg kg day, are expected to induce a biological effect
  • soluble type ⁇ IL- IR proteins are expected to have similar, if not identical, therapeutic activities.
  • soluble human type II IL-IR can be administered, for example, for the purpose of suppressing immune responses in a human.
  • diseases or conditions are caused by an immune response to alloantigen, including allograft rejection and graft-versus-host reaction.
  • shuIL-lR suppresses lymphoproliferation and inflammation which result upon activation of T cells.
  • shufl-lR can therefore be used to effectively suppress alloantigen-induced immune responses in the clinical treatment of, for example, rejection of all ⁇ grafts (such as skin, kidney, and heart transplants), and graft-versus-host reactions in patients who have received bone marrow transplants.
  • all ⁇ grafts such as skin, kidney, and heart transplants
  • graft-versus-host reactions in patients who have received bone marrow transplants.
  • Soluble human type II IL-IR can also be used in clinical treatment of autoimmune dysfunctions, such as rheumatoid arthritis, diabetes and multiple sclerosis, which are dependent upon the activation of T cells against antigens not recognized as being indigenous to the host.
  • Radioiodination yielded specific activities in the range of 1 x 10 15 to 5 x 10 15 cpm/mmol-1 (0.4 to 2 atoms I per molecule protein), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed a single labeled polypeptide of 17.5 kD, consistant with previously reported values for IL-1.
  • the labeled protein was greater than 98% TCA precipitable, indicating that the 125 I was covalently bound to protein.
  • a CB23 library was constructed and screened by direct expression of pooled cDNA clones in the monkey kidney cell line CV-l/EBNA-1 (which was derived by rransfection of the CV-1 cell line with the gene encoding EBNA-1, as described below) using a mammalian expression vector (pDC406) that includes regulatory sequences from SV40, human immunodeficiency virus (HIV), and Epstein-Barr virus (EBV).
  • pDC406 mammalian expression vector that includes regulatory sequences from SV40, human immunodeficiency virus (HIV), and Epstein-Barr virus (EBV).
  • the CV-l/EBNA-1 cell line constitutively expresses EBV nuclear antigen- 1 driven from the human cytomegalovirus (CMV) immediate-early enhancer/promoter and therefore allows the episomal replication of expression vectors such as pDC406 that contain the EBV origin of replication.
  • the expression vector used was pDC406, a derivative of HAV-EO, described by Dower et al., /. Immunol. 242:4314, 1989), which is in turn a derivative of pDC201 and allows high level expression in the CV-l/EBNA-1 cell line.
  • ⁇ DC406 differs from HAV-EO (Dower et al., supra) by the deletion of the intron present in the adenovirus 2 tripartite leader sequence in HAV-EO (see description of pDC303 below). .
  • the CB23 cDNA library was constructed by reverse transcription of poly(A) + mRNA isolated from total RNA extracted from the human B cell lymphoblastoid line CB23 (Benjamin & Dower, Blood 75:2017, 1990) substantially as described by Ausubel et al., eds., Current Protocols in Molecular Biology, Vol. 1, 1987.
  • the CB23 cell line is an EBV-transformed cord blood (CB) lymphocyte cell line, which was derived by using the methods described by Benjamin et al., Proc. Natl. Acad. Sci. USA 82:3547, 1984.
  • Poly(A) + mRNA was isolated by oligo dT cellulose chromatography and double-stranded cDNA was made substantially as described by Gubler and Hoffman.Gene 25:263, 1983. Briefly, the poly(A) + mRNA was converted to an RNA-cDNA hybrid with reverse transcriptase using random hexanucleotides as a primer. The RNA-cDNA hybrid was then converted into double- stranded cDNA using RNAase H in combination with DNA polymerase I. The resulting double stranded cDNA was blunt-ended with T4 DNA polymerase.
  • CV-l/EBNA-1 cells were derived as follows.
  • the CV- 1/EBNA-l cell line constitutively expresses EBV nuclear antigen- 1 driven from the CMV immediate-early enhancer/promoter.
  • the African Green Monkey kidney cell line, CV-1 (ATCC CCL 70, was cotransfected with 5 ⁇ g of pSV2gpt (Mulligan & Berg, Proc. Natl. Acad. Sci. USA 78:2072, 1981) and 25 ug of pDC303/EBNA-l using a calcium phosphate coprecipitation technique (Ausubel et al., eds., Current Protocols in Molecular Biology, Wiley, New York, 1987).
  • pDC303/EBNA-l was constructed from pDC302 (Mosley et al., Cell 59:335, 1989) in two steps. First, the intron present in the adenovirus tripartite leader sequence was deleted by replacing a PvuII to Seal fragment spanning the intron with the following synthetic oligonucleotide pair to create plasmid pDC303:
  • SEQ ID NO:5 5'-CTGTTGGGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCAGT-3' SEQ ID NO:6 3'-GACAACCCGAGCGCCAACTCCTGTTTGAGAAGCGCCAGAAAGGTCA-5'
  • EBNA-1 Epstein-Barr virus nuclear antigen I
  • EBNA-1 Epstein-Barr virus nuclear antigen I
  • the transfected cells were grown in the presence of hypoxanthine, aminopterin, thymidine, xanthine, and mycophenolic acid according to standard methods (Ausubel et al., supra; Mulligan & Berg, supra) to select for the cells that had stably incorporated the transfected plasmids.
  • the resulting drug resistant colonies were isolated and expanded individually into cell lines for analysis.
  • the cell lines were screened for the expression of functional EBNA-1.
  • One cell line, clone 68, was found to express EBNA-1 using this assay, and was designated CV-l/EBNA-1.
  • the cells were maintained in complete medium (Dulbecco's modified Eagle's media (DMEM) containing 10% (v/v) fetal calf serum (FCS), 50 U/ml penicillin, 50 U/ml streptomycin, 2 mM L- glutamine) and were plated at a density of 2 x 10 5 cells/well in either 6 well dishes (Falcon) or single well chambered slides (Lab-Tek). Both dishes and slides were pretreated with 1 ml human fibronectin (10 ug/ml in PBS) for 30 minutes followed by 1 wash with PBS.
  • DMEM Dulbecco's modified Eagle's media
  • FCS fetal calf serum
  • FCS fetal calf serum
  • PBS fetal calf serum
  • Both dishes and slides were pretreated with 1 ml human fibronectin (10 ug/ml in PBS) for 30 minutes followed by 1 wash with PBS.
  • transfected monolayers of CV-1/EBNA cells were assayed for expression of IL-1 binding proteins by binding radioiodinated IL-l ⁇ prepared as described above by slide autoradiography.
  • Transfected CV-l/EBNA-1 cells were washed once with binding medium (RPMI medium 1640 containing 25 mg/ml bovine serum albumin (BSA), 2 mg/ml sodium azide, 20 mM HEPES, pH 7.2, and 50 mg/ml nonfat dry milk (NFDM)) and incubated for 2 hours at 4°C with 1 ml binding medium + NFDM containing 3 x 10" 9 M 125 I- ⁇ L-l ⁇ .
  • binding medium RPMI medium 1640 containing 25 mg/ml bovine serum albumin (BSA), 2 mg/ml sodium azide, 20 mM HEPES, pH 7.2, and 50 mg/ml nonfat dry milk (NFDM)
  • This pool was further partitioned into pools of 75, plated in 6- well plates and screened by plate binding assays analyzed by quantitation of bound 125 I-IL-l ⁇ . The cells were scraped off the plates and counted to determine which pool of 75 was positive. Individual colonies from this pool of 75 were screened until a single clone (clone 75) was identified which directed synthesis of a surface protein with detectable IL-l ⁇ binding activity. This clone was isolated, and its insert was sequenced to determine the sequence of the human type II IL-IR cDNA clone 75.
  • a cDNA encoding a soluble human type II IL-IR (having the sequence of amino acids -13-333 of SEQ ID NO:l) was constructed by polymerase chain reaction (PCR) amplification using the full length type II IL-IR cDNA clone 75 (SEQ ID NO:l) in the vector pDC406 as a template.
  • the 5' primer corresponds to nucleotides 31-51 from the untranslated region of human type II IL-IR clone 75 (SEQ ID NO:l) with a 5' add-on of a Sail restriction site; this nucleotide sequence is capable of annealing to the (-) strand complementary to nucleotides 31-51 of human clone 75.
  • the 3' primer is complementary to nucleotides 1191-1172 (which includes anti-sense nucleotides encoding 3 amino acids of human type II IL-IR clone 75 (SEQ ID NO:l) and has a 5' add-on of a Not! restriction site and a stop codon.
  • PCR reagents were added to a 1.5 ml Eppendorf microfuge tube: 10 ⁇ l of 10X PCR buffer (500 mM KC1, 100 mM Tris-HCl, pH 8.3 at 25°C, 15 mM MgCl 2 , and 1 mg/ml gelatin) (Perkin-Elmer Cetus, Norwalk, CN), 10 ⁇ l of a 2mM solution containing each dNTP (2 mM dATP, 2 mM dCTP, 2 mM dGTP and 2 mM dTTP), 2.5 units (0.5 ⁇ l of standard 5000 units/ml solution) of Taq DNA polymerase (Perkin-Elmer Cetus), 50 ng of template DNA and 5 ⁇ l of a 20 ⁇ M solution of each of the above oligonucleotide primers and 74.5 ⁇ l water to a final volume of 100 ⁇ l.
  • 10X PCR buffer 500 mM KC1, 100 mM Tris-HC
  • PCR was carried out using a DNA thermal cycler (Ericomp, San Diego, CA) by initially denaturing the template at 94° for 90 seconds, reannealing at 55° for 75 seconds and extending the cDNA at 72° for 150 seconds. PCR was carried out for an additional 20 cycles of amplification using a step program (denaturation at 94°, 25 sec; annealing at 55°, 45 sec; extension at 72°, 150 sec), followed by a 5 minute extension at 72°.
  • a DNA thermal cycler Ericomp, San Diego, CA
  • the sample was removed from the paraffin oil and DNA extracted by phenol- chloroform extraction and spun column chromatography over G-50 (Boehringer Mannheim). A 10 ⁇ l aliquot of the extracted DNA was separated by electrophoresis on 1% SeaKemTM agarose (FMC BioProducts, Rockland, ME) and stained with ethidium bromide to confirm that the DNA fragment size was consistent with the predicted product
  • Murine type II BL-IR cDNAs were isolated from a cDNA library made from the murine pre-B cell line 70Z/3 (ATCC TIB 158), by cross species hybridization with a human Type II IL-IR probe.
  • a cDNA library was constructed in a ⁇ phage vector using ⁇ gtlO arms and packaged in vitro (Gigapack®, Stratagene, San Diego) according to the manufacturer's instructions.
  • a double-stranded human Type ⁇ IL-IR probe was produced by excising an approximately 1.35 kb Sail restriction fragment of the human type II IL-IR clone 75 and 32 P-labelling the cDNA using random primers (Boehringer-Mannheim).
  • the murine cDNA library was amplified once and a total of 5xl0 5 plaques were screened with the human probe in 35% formamide (5xSSC, 42°C).
  • Several murine type II BL-IR cDNA clones (including clone ⁇ 2) were isolated; however, none of the clones appeared to be full- length. Nucleotide sequence information obtained from the partial clones was used to clone a full-length murine type II IL-IR cDNA as follows.
  • a full-length cDNA clone encoding murine type II IL-IR was isolated by the method of Rapid Amplification of cDNA Ends (RACE) described by Frohman et al., Proc. Natl. Acad. Sci. USA 85:8998, 1988, using RNA from the murine pre-B cell line 70Z/3. Briefly, the RACE method uses PCR to amplify copies of a region of cDNA between a known point in the cDNA transcript (determined from nucleotide sequence obtained as described above) and the 3' end.
  • RACE Rapid Amplification of cDNA Ends
  • An adaptor-primer having a sequence containing 17 dT base pairs and an adaptor sequence containing three endonuclease recognition sites (to place convenient restriction sites at the 3' end of the cDNA) is used to reverse transcribe a population of mRNA and produce (-) strand cDNA.
  • a primer complementary to a known stretch of sequence in the 5' untranslated region of the murine type II IL-IR clone 2 cDNA, described above, and oriented in the 3' direction is annealed with the (-) strand cDNA and extended to generate a complementary (+) strand cDNA.
  • the resulting double-strand cDNA is amplified by PCR using primers that anneal to the natural 5'-end and synthetic 3'- end poly(A) tail. Details of the RACE procedure are as follows.
  • PCR oligonucleotide primers (d(T) ⁇ adaptor-primer, 5' amplification primer and 3' amplification primer, respectively) were first constructed:
  • the d(T)i7 adapter-primer contains nucleotide sequence anneals to the poly(A)+ region of a population mRNA transcripts and is used to generated (-) strand cDNA reverse transcripts from mRNA; it also contains endonuclease restriction sites for PstI, NotI and BamHI to be introduced into the DNA being amplified by PCR.
  • the 5' amplification primer corresponds to nucleotides 15-34 from the 5' untranslated region of murine type II IL-IR clone ⁇ 2 with a 5 * add-on of a Sail restriction site; this nucleotide sequence anneals to the (-) strand cDNA generated by reverse transcription with the d(T)i7 adaptor-primer and is extended to generate (+) strand cDNA.
  • the 3' primer (SEQ ID NO: 11) anneals to the (+) strand DNA having the above endonuclease restriction sites and is extended to generate a double-stranded full-length cDNA encoding murine type II BL-IR, which can then be amplified by a standard PCR reaction. Details of the PCR procedure are as follows.
  • Poly(A)+ mRNA was isolated by oligo dT cellulose chromatography from total RNA extracted from 70Z/3 cells using standard methods described by Maniatis et al., Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab., Cold Spring Harbor, NY, 1982) and reverse transcribed as follows.
  • Approximately 1 or 5 ⁇ l of the cDNA pool was combined with 5 ⁇ l of a 20 ⁇ M solution of the 5' amplification primer, containing sequence corresponding to the sequence of nucleotides 15-34 of murine type ⁇ IL-IR clone ⁇ 2, 5 ⁇ l of a 20 ⁇ M solution of the 3' amplification primer, 10 ⁇ l of 10X PCR buffer (500 mM KC1, 100 mM Tris-HCl (pH 8.4, 20°C), 14 mM MgCt ⁇ , and 1 mg/ml gelatin), 4 ⁇ l of 5mM each dNTP (containing 5 mM dATP, 5mM dCTP, 5mM dGTP and 5mM dTTP), 2.5 units (0.5 ⁇ l of standard 5000 units/ml solution) of Taq DNA polymerase (Perkin-Elmer Cetus Instruments), diluted to a volume of 100 ⁇ l.
  • 10X PCR buffer 500 mM
  • PCR was carried out using a DNA thermal cycler (Perkin-Elmer/Cetus) by initially denaturing the template at 94° for 90 seconds, reannealing at 64° for 75 seconds and extending the cDNA at 72° for 150 seconds. PCR was carried out for an additional 25 cycles of amplification using the following step program (denaturation at 94° for 25 sec; annealing at 55° for 45 sec; extension at 72° for 150 sec), followed by a 7 minute final extension at 72°.
  • a DNA thermal cycler Perkin-Elmer/Cetus
  • the sample was removed from the paraffin oil and DNA extracted by phenol- chloroform extraction and spun column chromatography over G-50 (Boehringer Mannheim). A 10 ⁇ l aliquot of the extracted DNA was separated by electrophoresis on 1% SeaKemTM agarose (FMC BioProducts, Rockland, ME) and stained with ethidium bromide to confirm that the DNA fragment size was consistent with the predicted product. The gel was then blotted and probed with a 5' 610bp EcoRI fragment of murine type II IL-IR clone ⁇ 2 from above to confirm that the band contained DNA encoding murine type II IL-IR.
  • the PCR-amplified cDNA products were then concentrated by centrifugation in an Eppendorf microfuge at full speed for 20 min., followed by ethanol precipitation in 1/10 volume sodium acetate (3 M) and 2.5 volume ethanol. 30 ⁇ l of the concentrate was digested with Sail and Notl restriction enzymes using standard procedures. The S all No tl restriction fragment was then separated on a 1.2% LGT agarose gel, and the band representing the fragment was isolated. The restriction fragments were then purified from the agarose using GeneCleanTM (Bio-101, La Jolla, CA).
  • the resulting purified restriction fragment was ligated into the pDC406 vector, which was then transfected into CV1-EBNA cells and expressed as described above in Example 1.
  • a cDNA encoding soluble murine type II IL-IR (having the sequence of amino acids -13-345 of SEQ ID NO: 12) was constructed by PCR amplification 70Z/3 poly(A) + mRNA as a template and the following procedure as described for the full length clone encoding murine type II IL-IR.
  • the following PCR oligonucleotide primers (d(T) ⁇ adaptor-primer, 5' amplification primer and 3' amplification primer, respectively) were constructed:
  • the d(T)i7 adaptor-primer and 5' amplification primer are identical with SEQ ID NO:9 and SEQ ID NO: 10, described in Example 5.
  • the 3' end of SEQ ID NO: 12 is complementary to nucleotides 1145-1166 of SEQ ID NO:12 and has a 5' add-on of a Notl restriction site and a stop codon.
  • a pool of cDNA was synthesized from poly(A) + mRNA using the d(T) ⁇ adaptor- primer as described in Example 3.
  • To a 1.5 ml Eppendorf microfuge tube was added approximately 1 ⁇ l of the cDNA pool, 5 ⁇ l of a 20 ⁇ M solution of the 5' amplification primer, 5 ⁇ l of a 20 ⁇ M solution of the 3' amplification primer, 10 ⁇ L of 10X PCR buffer (500 mM KC1, 100 mM Tris-HCl (pH 8.4 at 20°C), 14 mM MgCl 2 , and 1 mg/ml gelatin), 4 ⁇ l of 5mM each of dNTP (containing 5 mM dATP, 5mM dCTP, 5mM dGTP and 5mM dTTP), 2.5 units (0.5 ⁇ l of standard 5000 units/ml solution) of Taq DNA polymerase (Perkin-Elmer Cetus Instruments), diluted with 75.4
  • PCR was carried out using a DNA thermal cycler (Ericomp) by initially denaturing the template at 94° for 90 seconds, reannealing at 55° for 75 seconds and extending the cDNA at 72° for 150 seconds. PCR was carried out for an additional 20 cycles of amplification using the following step program (denaturation at 94° for 25 sec; annealing at 55° for 45 sec; extension at 72° for 150 sec), followed by a 7 minute final extension at 72°.
  • Ericomp DNA thermal cycler
  • the sample was removed from the paraffin oil and DNA extracted by phenol- chloroform extraction and spun column chromatography over G-50 (Boehringer Mannheim). A 10 ⁇ l aliquot of the extracted DNA was separated by electrophoresis on 1% SeaKemTM agarose (FMC BioProducts, Rockland, ME) and stained with ethidium bromide to confirm that the DNA fragment size was consistent with the predicted product.
  • the PCR-amplified cDNA products were then concentrated by centrifugation in an Eppendorf microfuge at full speed . for 20 min., followed by ethanol precipitation in 1/10 volume sodium acetate (3 M) and 2.5 volume ethanol. 50 ⁇ l was digested with Sail and Notl restriction enzymes using standard procedures. The Sall/NotI restriction fragment was then separated on a 1.2% Seaplaque LGT agarose gel, and the band representing the fragment was isolated. The restriction fragment was then purified from the isolated band using the following freeze/thaw method. The band from the gel was split into two 175 ⁇ l fragments and placed into two 1.5 ml Eppendorf microfuge tubes.
  • isolation buffer (0.15 M NaCl, 10 mM Tris, pH 8.0, ImM EDTA) was added to each tube and the tubes heated to 68°C to melt the gel. The gels were then frozen on dry ice for 10 minutes, thawed at room temperature and centrifuged at 4°C for 30 minutes. Supernatants were then removed and placed in a new tube, suspended in 2 mL ethanol, and centrifuged at 4°C for an additional 30 minutes to form a DNA pellet. The DNA pellet was washed with 70% ethanol, centrifuged for 5 minutes, removed from the tube and resuspended in 20 ⁇ l TE buffer.
  • the competitor binds to the receptor and prevents the radiolabeled ligand from binding to the receptor. Binding assays were performed by a phthalate oil separation method essentially as describe by Dower et al., /. Immunol. 132:151, 1984 and Park et al., /. Biol. Chem. 261:4177, 1986.
  • CV1/EBNA cells were incubated in six-well plates (Costar, Cambridge, MA) at 4°C for 2 hours with 125 I-BL-l ⁇ in 1 ml binding medium (Roswell Park Memorial Institute (RPMI) 1640 medium containing 2% BSA, 20 mM Hepes buffer, and 0.2% sodium azide, pH 7.2). Sodium azide was included to inhibit internalization and degradation of 125 I-IL-1 by cells at 37 ⁇ C. The plates were incubated on a gyratory shaker for 1 hour at 37°C.
  • RPMI Roswell Park Memorial Institute
  • Replicate aliquots of the incubation mixture were then transferred to polyethylene centrifuge tubes containing a phthalate oil mixture comprising 1.5 parts dibutylphthalate, to 1 part bis(s-ethylhexyl)phthalate.
  • Control tubes containing a lOOX molar excess of unlabeled IL-l ⁇ were also included to determine non-specific binding.
  • the cells with bound 125 I-IL-1 were separated from unbound 125 I-IL-1 by centrifugation for 5 minutes at 15,000X g in an Eppendorf Microfuge. The radioactivity associated with the cells was then determined on a gamma counter.
  • This assay (using unlabeled human IL-l ⁇ as a competitor to inhibit binding of 125 I-IL-l ⁇ to type II BL-IR) indicated that the full length human type II IL-IR exhibits biphasic binding to IL-l ⁇ with a K ⁇ of approximately 19 ⁇ 8 x 10 9 and K ⁇ 2 of approximately 0.2 ⁇ 0.002 x 10 9 .
  • the full length human type II IL-IR exhibited biphasic binding to IL-l ⁇ with a K ⁇ of approximately 2.0 ⁇ 1 x 10 9 and K ⁇ of approximately 0.013 ⁇ 0.003 x 10 9 .
  • the binding inhibition constant of the soluble human type II IL-IR, expressed and purified as described in Example 2 above, is determined by a inhibition binding assay in which varying concentrations of an IL-l ⁇ competitor is incubated with a constant amount of radiolabeled I-EL-l ⁇ and CB23 cells (an Epstein Barr virus transformed cord blood B lymphocyte cell line) expressing the type II IL-IR. Binding assays were also performed by a phthalate oil separation method essentially as describe by Dower et al., /. Immunol. 232:751, 1984 and Park et al., J. Biol. Chem. 261:4177, 1986.
  • COS-7 cells were transfected with the expression vector pDC406 containing a cDNA encoding the soluble human type II IL-IR described above.
  • Supernatants from the COS cells were harvested 3 days after transfection and serially diluted in binding medium (Roswell Park Memorial Institute (RPMI) 1640 medium containing 2% BSA, 20 mM Hepes buffer, and 0.2% sodium azide, pH 7.2) in 6 well plates to a volume Of 50 ⁇ l well.
  • the supernatants were incubated with 50 ⁇ l of 9xl0- 1 ⁇ > M l25l-IH ⁇ plus 2.5X10 6 CB23 cells at 8°C for 2 hours with agitation.
  • Duplicate 60 ⁇ l aliquots of the incubation mixture were then transferred to polyethylene centrifuge tubes containing a phthalate oil mixture comprising 1.5 parts dibutylphthalate, to 1 part bis(s-ethylhexyl)phthalate.
  • a negative control tube containing 3xl0" 6 M unlabeled IL-l ⁇ was also included to determine non-specific binding (100% inhibition) and a positive control tube containing 50 ml binding medium with only radiolabeled IL-l ⁇ was included to determine maximum binding.
  • the cells with bound 125 I-IL-l ⁇ were separated from unbound 125 I-EL-l ⁇ by centrifugation for 5 minutes at 15,000 X g in an Eppendorf Microfuge.
  • Murine type II IL-IR exhibits biphasic binding to IL-l ⁇ with a Kn of 0.8 x 10 9 and a K 1 2 of less then 0.01 x 10 9 .
  • Recombinant human IL- 1 receptor antagonist (EL- Ira) was cloned using the cDNA sequence published by Eisenberg et al., Nature 343:341, 1990, expressed by transient transfection in COS cells, and purified by affinity chromatography on a column of soluble human type I IL-IR coupled to affigel, as described by Dower et al., J. Immunol. 243:4314, 1989, and eluted at low pH.
  • CV1/EBNA cells (4 x 10 7 /ml) expressing recombinant type II IL-IR were incubated with 125 I-IL-l ⁇ or 125 I-IL-l ⁇ (1 nM) at 4°C in the presence and absence of 1 ⁇ M excess of unlabeled IL-1 as a specificity control for 2 hours. The cells were then washed and bis(sulfosuccinimidyl)suberate was added to a final concentration of 0.1 mg ml. After 30 min.
  • the cells were washed and resuspended in 100 ⁇ l of phosphate-buffered saline (PBS)/1% Triton containing 2 mM leupeptin, 2 mM o-phenanthroline, and 2 mM EGTA to prevent proteolysis.
  • PBS phosphate-buffered saline
  • Triton containing 2 mM leupeptin, 2 mM o-phenanthroline, and 2 mM EGTA to prevent proteolysis.
  • Figure 4 shows the results of affinity crosslinking studies conducted as described above, using radiolabeled IL-l ⁇ and IL-l ⁇ , to compare the sizes of the recombinant murine and human type II IL-1 receptor proteins to their natural counterparts, and to natural and recombinant murine and human type I IL-1 receptors.
  • the sizes of the transiently-expressed recombinant receptors are similar to the natural receptors, although the recombinant proteins migrate slightly faster and as slightly broader bands, possibly as a result of differences in glycosylation patter when over-expressed in CV1/EBNA cells.
  • the results also indicate that the type II EL-l receptors are smaller than the type I IL-1 receptors.
  • Preparations of purified recombinant type II IL-IR for example, human type II BL- IR, or transfected COS cells expressing high levels of type II IL-IR are employed to generate monoclonal antibodies against type II IL-IR using conventional techniques, for example, those disclosed in U.S. Patent 4,411,993.
  • Such antibodies are likely to be useful in interfering with IL-1 binding to type II IL-IR, for example, in ameliorating toxic or other undesired effects of EL-l, or as components of diagnostic or research assays for IL-1 or soluble type II IL-IR.
  • type II IL-IR immunogen is emulsified in complete Freund's adjuvant and injected in amounts ranging from 10-100 ⁇ g subcutaneously and interaperitoneally into Balb/c mice.
  • the immunized animals are boosted with additional immunogen emulsified in incomplete Freund's adjuvant and periodically boosted thereafter on a weekly to biweekly immunization schedule.
  • Serum samples are periodically taken by retro-orbital bleeding or tail-tip excision for testing by dot-blot assay (antibody sandwich) or ELISA (enzyme-linked immunosorbent assay), or receptor binding inhibition. Other assay procedures are also suitable.
  • Hybridoma cell lines generated by this procedure are plated in multiple microtiter plates in a HAT selective medium (hypoxanthine, aminopterin, and thymidine) to inhibit proliferation of non-fused cells, myeloma hybrids, and spleen cell hybrids.
  • HAT selective medium hyperxanthine, aminopterin, and thymidine
  • Hybridoma clones thus generated can be screened by ELISA for reactivity with type II IL-IR, for example, by adaptations of the techniques disclosed by Engvall et al., Immunochem. 8:871 (1971) and in U.S. Patent 4,703,004. Positive clones are then injected into the peritoneal cavities of syngeneic Balb/c mice to produce ascites containing high concentrations (>1 mg/ml) of anti-type II IL-IR monoclonal antibody, or grown in flasks or roller bottles.
  • the resulting monoclonal antibody can be purified by ammonium sulfate precipitation followed by gel exclusion chromatography, and/or affinity chromatography based on binding of antibody to Protein A of Staphylococcus aureus or protein G from Streptococci.
  • SEQ ID NO:l and SEQ ID NO:2 show the nucleotide sequence and predicted amino acid sequence of human type II IL-IR.
  • the mature peptide encoded by this sequence is defined by amino acids 1-385.
  • the predicted signal peptide is defined by amino acids -13 through -1.
  • the predicted transmembrane region is defined by amino acids 331-356.
  • SEQ ID NO:3 - SEQ ID NO:6 are various oligonucleotides used to clone the full- length human type II IL-IR.
  • SEQ ID NO:7 and SEQ ID NO: 8 are oligonucleotide primers used to construct a soluble human type II IL-IR by polymerase chain reaction (PCR).
  • SEQ ED NO:9 - SEQ ID NO: 11 are oligonucleotide primers used to clone a full- length and soluble murine type II IL-IRs.
  • SEQ ID NO: 12 and SEQ ID NO: 13 show the nucleotide sequence and predicted amino acid sequence of the full-length murine type II IL-IR.
  • the mature peptide encoded by this sequence is defined by amino acids 1-397.
  • the predicted signal peptide is defined by amino acids -13 through -1.
  • the predicted transmembrane region is defined by amino acids 343-368.
  • SEQ ID NO: 14 is an oligonucleotide primer used to construct a soluble murine type ⁇ IL-IR.
  • GCT GCC AGA AGC TGC CGG TTT CGT GGG AGG CAT TAC AAG CGG GAG TTC 270 Ala Ala Arg Ser Cys Arg Phe Arg Gly Arg His Tyr Lys Arg Glu Phe 15 20 25
  • ATC TCA TAC CCG CAA ATT TTA ACC TTG TCA ACC TCT GGG GTA TTA GTA 606 lie Ser Tyr Pro Gin lie Leu Thr Leu Ser Thr Ser Gly Val Leu Val 125 130 135
  • Asp Lys Thr Asp Val Lys lie Gin Trp Tyr Lys Asp Ser Leu Leu Leu 150 155 160
  • Trp Trp Thr Ala Asn Asp Thr His lie Glu Ser Ala Tyr Pro Gly Gly 260 265 270 275
  • Tyr lie Glu Val Pro Leu lie Phe Asp Pro Val Thr Arg Glu Asp Leu 295 300 ' 305
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE N
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE N
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE Y
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE N
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE N
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE N
  • SEQUENCE DESCRIPTION SEQ ID NO:9: CTGCAGGCGG CCGCGGATCC TTTTTTTTTT TTTTTTT 37
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE N
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE N
  • Trp lie Leu Pro Ala Val-Gin Gin Asp Ser Gly Thr Tyr lie Cys Thr 95 100 105
  • Trp Ser lie Ala Leu Ala Pro Leu Ser Leu lie lie Leu Val Val Gly 350 355 360 GCA ATA TGG ATG CGC AGA CGG TGT AAA CGC AGG GCT GGA AAG ACA TAT 1263 Ala He Trp Met Arg Arg Arg Cys Lys Arg Arg Ala Gly Lys Thr Tyr 365 370 375 380
  • MOLECULE TYPE DNA (genomic)
  • HYPOTHETICAL N
  • ANTI-SENSE N

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Abstract

Protéines de récepteurs de IL-1 (IL-1R type II) de type II, ADN et vecteurs d'expression codant L'IL-1R de type II, et procédés de production de IL-1R de type II comme produits de culture de cellules recombinées.
PCT/US1991/003498 1990-06-05 1991-05-17 Recepteurs de l'interleukine-1 de type ii Ceased WO1991018982A1 (fr)

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AU79081/91A AU651596B2 (en) 1990-06-05 1991-05-17 Type II interleukin-1 receptors

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US53419390A 1990-06-05 1990-06-05
US534,193 1990-06-05
US57357690A 1990-08-24 1990-08-24
US573,576 1990-08-24
US62707190A 1990-12-13 1990-12-13
US627,071 1990-12-13

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EP0460846A1 (fr) 1991-12-11
IL98205A0 (en) 1992-06-21
NZ238255A (en) 1992-03-26
DE69132937D1 (de) 2002-04-04
JP3652582B2 (ja) 2005-05-25
US20030060616A1 (en) 2003-03-27
AU651596B2 (en) 1994-07-28
IE911771A1 (en) 1991-12-18
ATE213743T1 (de) 2002-03-15
AU7908191A (en) 1991-12-31
JP3155024B2 (ja) 2001-04-09
IL98205A (en) 1998-01-04
JPH0767651A (ja) 1995-03-14
JP2000336039A (ja) 2000-12-05
ES2172502T3 (es) 2002-10-01
EP0460846B1 (fr) 2002-02-27
DE69132937T2 (de) 2002-09-19
DK0460846T3 (da) 2002-06-10

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