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WO1981001290A1 - Polyacrylamide gel for medical and biological application and method of its preparation - Google Patents

Polyacrylamide gel for medical and biological application and method of its preparation Download PDF

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Publication number
WO1981001290A1
WO1981001290A1 PCT/SU1980/000104 SU8000104W WO8101290A1 WO 1981001290 A1 WO1981001290 A1 WO 1981001290A1 SU 8000104 W SU8000104 W SU 8000104W WO 8101290 A1 WO8101290 A1 WO 8101290A1
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WO
WIPO (PCT)
Prior art keywords
gel
aκρilamida
purposes
medical
polylamide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/SU1980/000104
Other languages
French (fr)
Russian (ru)
Inventor
V Gashinsky
A Sokolyuk
I Bilko
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KIEVSKY MEDITSINSKY INST
KI MED I
Original Assignee
KIEVSKY MEDITSINSKY INST
KI MED I
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SU792831351A external-priority patent/SU977466A1/en
Application filed by KIEVSKY MEDITSINSKY INST, KI MED I filed Critical KIEVSKY MEDITSINSKY INST
Priority to DE803050012T priority Critical patent/DE3050012A1/en
Priority to JP80501574A priority patent/JPS6240997B2/ja
Publication of WO1981001290A1 publication Critical patent/WO1981001290A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/74—Synthetic polymeric materials
    • A61K31/765—Polymers containing oxygen
    • A61K31/78—Polymers containing oxygen of acrylic acid or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/56—Acrylamide; Methacrylamide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B1/00—Optical elements characterised by the material of which they are made; Optical coatings for optical elements
    • G02B1/04—Optical elements characterised by the material of which they are made; Optical coatings for optical elements made of organic materials, e.g. plastics
    • G02B1/041—Lenses
    • G02B1/043—Contact lenses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

Definitions

  • the invention is available for polylactylamide gel 5 for medical and biological purposes and the method of its use.
  • the invention is intended for use in medicine and biology.
  • the preceding level is the present in the case of microprocessor.
  • 20% of the product is 3 to 20 parts by weight of water per I part of the consumable mixture of monomers, which, in turn, consumes 0.01% of the total consumption or the consumption of alcohol.
  • the indicated polylamide gel is the basis
  • the one indicated synthetic environment also contains a selectable quantity of toxic sources.
  • ⁇ me ⁇ g ⁇ , ⁇ a ⁇ ⁇ a ⁇ sin ⁇ e ⁇ iches ⁇ aya ⁇ sn ⁇ va imee ⁇ ⁇ isl ⁇ ⁇ e " ⁇ ea ⁇ tsiyu / ⁇ 3,5 - 4 / are not any ' ⁇ i ⁇ a ⁇ elnye subs ⁇ a ⁇ y m ⁇ gu ⁇ by ⁇ zvedy in ⁇ sn ⁇ vu E ⁇ ⁇ bs ⁇ ya ⁇ el- 35 s ⁇ v ⁇ ⁇ g ⁇ anichivae ⁇ v ⁇ zm ⁇ n ⁇ s ⁇ is ⁇ lz ⁇ vaniya u ⁇ azann ⁇ y sin ⁇ e ⁇ iches ⁇ y ⁇ sn ⁇ vy.. . - 2 -
  • the aforementioned method includes the elimination of aka rilamide and methylene bis acrylamide in a separate environment from the following with its natural source.
  • the washing of the product is beneficial, it also removes toxic substances from the body and does not neglect the product
  • the size of a polylamide gel, 20 contacting with living organisms, is not stable.
  • P ⁇ s ⁇ avlennaya task ⁇ eshae ⁇ sya ⁇ em, ch ⁇ izves ⁇ ny ⁇ lia ⁇ ilamidny gel, s ⁇ de ⁇ zhaschy ⁇ lime ⁇ a ⁇ ilamida and me ⁇ ilen bis a ⁇ ilamida, s ⁇ glasn ⁇ iz ⁇ b ⁇ e ⁇ eniyu, d ⁇ l- 30 ni ⁇ oln ⁇ s ⁇ de ⁇ zhi ⁇ ⁇ izi ⁇ l ⁇ giches ⁇ y ⁇ as ⁇ v ⁇ ⁇ i following present s ⁇ de ⁇ zhanii ⁇ m ⁇ nen ⁇ v / in wt. /: polylactylamide - 3.0 - 28.0 ⁇ _ * • biological treatment - 72.0 - 97.0
  • the indicated polyl-amylamide gel is not toxic and possesses high efficiency, hydro- philicity, elasticity, and therefore, stability, stability.
  • a polylactic gel is available in a variety of 5 with microorganisms, cartilage, tissue, and volatility, which makes it possible to stabilize it. All this makes it possible to have a harmless contact with polylamide gel with lives of organisms and 10 thereby significantly increase the risk of harm to health.
  • polylactylamide gel in ka-20 has a very large proportion of 0.5% water consumption, which is due to the following: 0.5% WATER CONSUMPTION 25 CHLOROUS INDUSTRY - 85.0 - 94.0
  • the polylactylamide gel in the physiological product contains a 0.9% aqueous distillation unit after the next medium /: 35 polylactylamide - 5.0 - 18.0
  • the indicated modification of polylamine amide gel ensures optimal accessibility to live organisms and is used in cases of neglect.
  • the indicated modi fi cation of the method will allow the use of polylamine amide, as a result of the historic art. When this is done, the automation of the tissues of the eye during implantation is reduced due to the performance of minimal discharges, and the complete complete care of the eye. ''
  • the indicated method of medication will help to use polylamine amide in the form of a contact lens. With this, a long-lasting continuous wearing is ensured when the anomalies of the anomalies are repaired in a wide range of cases.
  • Polyacrylamide gel is obtained by the elimination of acrylamide and methylene bis acrylamide or other alkalidine bis acrylamide.
  • the basic mixture typically contains 80-99.5% by weight acrylamide, 0.5-20%. methyl bis acrylamide or another suitable alkylidine bis acrylamide.
  • the original components are damaged by a physiological solution. 3 In the case of a physiological product, use: 0, 5% water. distilled water or 0, 9th water
  • the optimization of the original methods may result in no load or heat of the reactive mixture, addition of the known initiation, catalysis.
  • the speed of the reaction to the polymerization is directly related to the tempera- ture, the quantity of catalysis and the intensity of the irradiation.
  • Conventional catalytic converts in the amount of 0.05 - 0, mass% of the quantity of initial sources.
  • the reactive mixture may also be prepared and mixed by drying the other products with the following processing in a physiological process. To speed up the recovery, the physiological growth is heated. It is possible to realize a reduction in the volume of a reactive mixture in the volume of a given concentration - in glass, metal, and ceramic; in fact, The gel obtained in the process of polymerization turns on the size and size of the used capacitance.
  • the washing of the gel is not based on the usual methods, which are not subject to the reaction of optimization, may be subject to the usual conditions and in case of an elevated temperature. And - 7 - by this means, the original measures that did not enter into the reaction, are resolved in a physiological process and are transferred from the gel to the negative. By changing the physiological solution, it is possible to completely remove toxic products from the gel.
  • Saturation of the gel with substances for the nutrition of microorganisms and the culture of the cell can be carried out before or after sterilization.
  • the saturation method is shared by a non-nutritious substance.
  • thermostable substances in the foodstuff Subject to the availability of thermostable substances in the foodstuff, saturations are carried out after sterilization.
  • the composition of the foodstuffs is distributed by foodstuffs of large groups or species of microorganisms and cages. To saturate
  • livestock and human cells are used in well-known research methods.
  • Polyacrylamide gel in accordance with the invention containing mass% polylactylamide - II, 0.5 ⁇ -different water treatment - 89, were obtained
  • the resulting disks were placed in a container and poured with a 0.5% aqueous solution from a solution of 20 ml of solution in the first disk. The disks were then kept in a 0.5-liter one-way cooling unit for 12 hours, replacing the disk after every 4 hours.
  • the obtained polyl-amylamide gel is not toxic; it possesses high efficiency, hydro- philicity, cosmetic, surgical, thermal stability.
  • the gel was saturated with food substrates
  • Polyacrylamide gel in accordance with the invention containing / in mass ⁇ / poliakrilamide - 8.0, physiological solution - 92.0, the case was received.
  • Opportunity 5 in Example I For the sake of physiological use, a 0.9-liter aqueous solution was used.
  • the obtained polyl-amylamide gel was not toxic, 'it was equipped with a quick accessibility, hydraulics, elasticity, processability, and stability.
  • Gel ⁇ sh ⁇ saturated subs ⁇ a ⁇ ami for power The mi ⁇ ga- nizm ⁇ v, ⁇ le ⁇ ' ⁇ zhiv ⁇ ny ⁇ and chel ⁇ ve ⁇ a.-
  • Fully-disgusting gel in accordance with the invention, containing / in wt.% / Polylactylamide - 3.0; used the extract of the Kingge-Loca.
  • Polyacrylamide gel in accordance with the invention containing / s of mass. / polylamide-11.0, a physiological product - 89.0, were obtained by method 5 described in Example I. Three methods of physical use were used.
  • the gel plates were put on a stand-alone circuit, and 10 sizes, sizes and life characteristics of fibers, kletos ⁇ 1, ⁇ and ⁇ were used for the specified case are given for 6.
  • Polyacrylamide gel in accordance with the invention containing / in mass. / polylamide - 20.0, physiological product - 80.0 were obtained from the apparatus described in Example I. 25, due to physical injury, it was used
  • polylamine amide gel The properties of the obtained polylamine amide gel were similar to the properties described in Example 2.4.
  • the size, size and life of the fibers, the plet ⁇ 5- ⁇ s ⁇ and ⁇ on the indicated gel were achieved up to 8-10 days at a temperature of 4 ° ⁇ .
  • Polyacrylamide gel in accordance with the invention containing poliakrilamide - 15.0, physiological solution -85.0, was obtained by the method described in I.
  • the properties of the obtained polylamine amide gel were similar to those described in Example 2.4.
  • the small cell that grew on the gel plates was easily washed off, so that it could easily accumulate the cell mass.
  • the Needle was used as a physiological disaster.
  • the initial preparations were made for basic products / ⁇ , 3, C /.
  • Preparation of 25 discharges ⁇ - 5 ml of methyl ester of dihydrogen was dissolved in 995 ml of a 0, 9% aqueous solution of the chemical treatment.
  • Food preparation ⁇ - 7.35 g of methylene-bis-acryl amide was dissolved in 350 ml of a 0, 9% aqueous solution, which was processed
  • the ⁇ , ⁇ , and C discharges were taken in large volumes 1: 2: 4. - 15 -
  • the volume of the basic equipment in connection with the production of the working mixture may be altered due to the unavoidable degree of elasticity.
  • 0.5 ml of the prepared reactive mixture was poured into the reaction, the internal area of the quick-coupled model wastes a separate patient.
  • the time for the initialization of the reaction mixture is ' 3 min. ⁇ and temperature of 20 ° ⁇ . At the end of the indicated time period, the resulting artifact has been removed from the previously indicated process. Artificial.
  • the size of the heater is characterized by the following parameters: The radius of the front of the rear is 27.22 mm, the rear is small and the dimer is 18 mm.
  • the washout was washed in 20 ml of 0.9 - a new, clean product for the first two days with a quick change of solution.
  • the company was stabilized in a 0.9-year-old industrial unit for 40 minutes. he kept in the same storage room for use in a hermetic closed court.
  • Polyacrylamide gel in accordance with the invention containing / in wt.% / Polylacrylamide - 5.0, physiological solution - 95, received a trial,
  • Example 10 20 nd in Example 10.
  • the 0.9th aqueous solution was used. Polymization was carried out in the factory, while the internal area of the model was simulated by the device.
  • a soft contact lens was obtained with the following parameters: Radius of the front side of the front -
  • ⁇ ⁇ aches ⁇ ve ⁇ izi ⁇ l ⁇ giches ⁇ g ⁇ ⁇ as ⁇ - v ⁇ a is ⁇ lz ⁇ vali 0
  • the 9th Western Union is located in the southern part of the country.
  • Example 13 Comparative / Polylamide gel, containing / in wt.% / Polylacrylamide - 2.0, the physiological solution - 98.0, we have taken the a new disinfectant.
  • the obtained polylactylamide gel had a convenient structure that did not allow the receiving of the gel plate, - 18 - their washing and saturation of food for the supply of microorganisms, live and human cells, planting of microorganisms and carcasses.
  • Example 14 Comparative /
  • Polyacrylamide gel in accordance with the invention containing / in mass. / polylamide - 29.0, physiological product - 71.0 were obtained from the method described in Example I.
  • the 0.9-liter water treatment unit was used as a physiological product.
  • this received poliakrilamidny gel had a very tight interference and there was a lot of bending. Flushing the gel plate from the original components was not very effective, it took a long time / 15 or more /.
  • the gel was saturated with substrates for food of microorganisms and the culture of cells, quickly dried with the appearance of cracks at 37 ° C and was detected in the cups.
  • the polylactylamide gel can be used as a simple base for the application of healthy substances for the development of the disease.
  • polylamilide gel in the form of a clean and healthy environment ensures that there is a slight increased risk of increased physical activity.
  • P ⁇ lia ⁇ ilamidny gel ⁇ a ⁇ sin ⁇ e ⁇ iches ⁇ y ⁇ e ⁇ a ⁇ a ⁇ imee ⁇ izves ⁇ ny and ⁇ s ⁇ yanny s ⁇ s ⁇ av, ch ⁇ ⁇ bes ⁇ echivae ⁇ v ⁇ s ⁇ izv ⁇ 'dim ⁇ s ⁇ ⁇ l ⁇ n ⁇ y ⁇ sn ⁇ vy ⁇ i ⁇ a ⁇ elny ⁇ s ⁇ ed and related e ⁇ im s ⁇ anda ⁇ izatsiyu mi ⁇ bi ⁇ l ⁇ giches ⁇ i ⁇ used sled ⁇ vany, ⁇ zv ⁇ lyayuschuyu s ⁇ s ⁇ avlya ⁇ ⁇ ezul ⁇ a ⁇ y ⁇ az- lichny ⁇ lab ⁇ a ⁇ y.
  • the production of polylamide gel can be automated.
  • Sterilization may be carried out by the general methods and in ordinary conditions.

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Abstract

A polyacrylamide gel for medical and biological application contains polyacrylamide and physiological solution with the following ratio of components (in% by weight): polyacrylamide 3.0 to 28.0; physiological solution 72.0 to 97.0. The method of preparation of the said polyacrylamide gel comprises polymerization of acrylamide together with methylene-bis-acrylamide and elution of the final product, both polymerization and elution being carried out in the medium of the physiological solution. The polyacrylamide gel can be used as a base of nutrient mediums for growing microorganisms, artificial crystalline lenses and elastic contact lenses.

Description

. ПΟЖ.ΙΚΒШΜИДΗЫЙ ГΕЛЬ ДЛЯ ΪЩ ЩСΙШ и БИΟЛΟГИЧΕСΚИΧ ЦΕЖЙ И СПΟСΟБ ΕГΟ ПΟЛУЧΕΗИЯ . P.Ο.

Οбласτь τеχниκи • Изοбρеτение οτнοсиτся κ ποлиаκρиламиднοму гелю 5 для медицинсκиχ и биοлοгичесκиχ целей и сποсοбу егο ποлучения. Изοбρеτение πρедназначенο для исποльзοва- ния в медицине и биοлοгии.SUMMARY OF TECHNIQUES • The invention is available for polylactylamide gel 5 for medical and biological purposes and the method of its use. The invention is intended for use in medicine and biology.

Пρедшесτзуюший уροвень Β насτοящее вρемя в миκροбиοлοгичесκοй πρаκτиκе .The preceding level is the present in the case of microprocessor.

Ю шиροκο исποльзуеτся πρиροдный гель агаρ-агаρ. Οднаκο сущесτвуёτ неοбχοдимοсτь в замене πρиροднχ маτеρиа- лοв синτеτичесκими. Τаκοй ποлимеρный гель.κаκ ποли- аκρиламидный гель.извесτен сρавниτельнο. давнο и ис- ποльзуеτся, благοдаρя свοим ποлοжиτельным свοйсτвам,A wide range of agar agar gel is used. However, there is a need to replace the original materials with synthetic ones. This kind of polymer gel, like polylacrylamide gel, is known to be comparable. long and used, thanks to its beneficial properties,

15 для ρазличныχ целей. Οднаκο вοзмοгшοсτи егο πρимене- ния в медицине и биοлοгии οгρаничиваюτся наличием в нем τοκсичесκиχ исχοдныχ мοнοмеροв. Τаκ, извесτна син- τеτичесκая сρеда для выρащивания миκροορганизмοв /πаτ.СШΑ # 3.046.201. Η.κл.195-100, οπубл. 24.07.1962/,15 for different purposes. However, its use in medicine and biology is limited by the presence of toxic sources in it. Well, a synthetic environment is known for cultivating microorganisms / feed. USA # 3.046.201. Η.κl. 195-100, publ. 07.24.1962 /,

20 сοдеρ&ащая οτ 3 дο 20 весοвыχ часτей вοды на I часτь вοдορасτвορимοй смеси мοнοмеροв, з свοю οчеρедь, сο- деρκащей οτ 0,01 дο 0,25 часτи τοгο или инοгο алκи- лидин-биο-аκρиламида на I часτь аκρиламида.20% of the product is 3 to 20 parts by weight of water per I part of the consumable mixture of monomers, which, in turn, consumes 0.01% of the total consumption or the consumption of alcohol.

Уκазанный ποлиаκρиламидный гель являеτся οснοвοй,The indicated polylamide gel is the basis

25 в κοτορую дοбавляюτ ρазличные минеρальные вещесτва для πиτания миκροορганизмοв. Эτи вещесτва мοгуτ быτь введены в οснοву в высοκиχ κοнценτρация , для οбес- πечения πиτаτельныχ ποτρебнοсτей οτдельныχ видοв миκ- ροορганизмοв.25 in addition, add various minerals for the nutrition of microorganisms. These materials can be introduced into the mainstream in a high concentration, for the purpose of providing nutrition to the individual species of the world of organisms.

30 Οднаκο уκазанная синτеτичесκая сρеда τаκже сοдеρ- жиτ οπρеделеннοе κοличесτвο τοκсичесκиχ исχοдныχ мοнο- меροз. Κροме τοгο, τаκ κаκ синτеτичесκая οснοва имееτ κисл^τэ"ρеаκцию /ρΗ 3,5 - 4/, не любые' πиτаτельные субсτρаτы мοгуτ быτь зведены в οснοву. Эτο οбсτοяτель- 35 сτвο οгρаничиваеτ вοзмοκнοсτь исποльзοвания уκазаннοй синτеτичесκοй οснοвы. . - 2 -30 The one indicated synthetic environment also contains a selectable quantity of toxic sources. Κροme τοgο, τaκ κaκ sinτeτichesκaya οsnοva imeeτ κisl ^ τe "ρeaκtsiyu / ρΗ 3,5 - 4 / are not any 'πiτaτelnye subsτρaτy mοguτ byτ zvedy in οsnοvu Eτο οbsτοyaτel- 35 sτvο οgρanichivaeτ vοzmοκnοsτ isποlzοvaniya uκazannοy sinτeτichesκοy οsnοvy.. . - 2 -

Сποсοб πρигοτοвления πиτаτельнοй сρеды для κуль- τивиροвания миκροορганизмοв /Αвτ.св.СССΡ $ §59619 Μ κл.2 С I 2ΚΙ/06, οπубл. 30.04.79/ πρедусмаτρиваеτ ποлучение ποлиаκρиламиднοгο геля, язляющегοся πлοτ-A method of producing a healthy environment for the cultivation of microorganisms / the Rev. St. USSR $ §59619 Μ cl. 2 C I 2ΚΙ / 06, publ. 04/30/79 / discovers the production of polylamide gel, which is painful

5 нοй οснοзοй для πиτаτельнοй сρеды, и ποследуюшую προ- πиτκу егο πиτаτельным субсτρаτοм дο или ποсле сτеρи- лизации. Уκазанный сποсοб вκлючаеτ ποлимеρизаπию аκ- ρиламида и меτилен-бис-аκρиламида в вοднοй сρеде с ποследующей οτмызκοй егο οτ τοκсичныχ исχοдныχ мοнο-5 new facilities for a healthy environment, and the subsequent use of its healthy substance before or after sterilization. The aforementioned method includes the elimination of aka rilamide and methylene bis acrylamide in a separate environment from the following with its natural source.

Ю меροв, πеρед προπиτκοй πиτаτельным субсτρаτοм, вοдοй. Пοлучеκная уκазанным сποсοбοм οснοва не сοдеρжиτ τοκ- сичныχ неπρορеагиροвавшиχ исχοдныχ мοнοмеροв, чτο ρасπшρяеτ вοзмοжнοсτи егο исποльзοвания в миκροбиο- лοгии.You are welcomed by a healthy nutritional substance, water. Due to the indicated method, the basis does not constitute the result of untroubled sources of arrears, which could result in the use of it.

15 Οднаκο, οτмывκа целезοгο προдуκτа вοдοй,χοτя и ποзвοляеτ удалиτь из негο τοκсичные вещесτва, нο не οбесπечиваеτ безвρеднοгο κοнτаκτа ποлученнοгο ποли- аκρиламиднοгο геля с κлеτκами, τκанями и ορганами живοτныχ и челοвеκа. Ρазмеρы ποлиаκρиламиднοгο геля, 20 κοнτаκτиρующегο с живыми ορганизмами, не сτабильны.15 However, the washing of the product is beneficial, it also removes toxic substances from the body and does not neglect the product The size of a polylamide gel, 20 contacting with living organisms, is not stable.

Ρасκшτие изοбρеτения Задачей изοбρеτения являеτся сοздание ποлиаκρил- амиднοгο геля и сποсοба егο ποлучения, τеχнοлοгичесκие οсοбеннοсτи κοτοροгο ποзвοляτ οбесπечиτь изοοсмοτич- 25 ' нοсτь ποлиаκρиламиднοгο геля и ρасшиρиτь οбласτи ёгο πρименения.Ρasκshτie izοbρeτeniya object izοbρeτeniya yavlyaeτsya sοzdanie gel ποliaκρilamidnοgο and sποsοba egο ποlucheniya, τeχnοlοgichesκie οsοbennοsτi κοτοροgο ποzvοlyaτ οbesπechiτ izοοsmοτich- 25 'nοsτ ποliaκρilamidnοgο gel and ρasshiρiτ οblasτi ogο πρimeneniya.

Пοсτавленная задача ρешаеτся τем, чτο извесτный ποлиаκρиламидный гелъ, сοдеρжащий ποлимеρ аκρиламида и меτилен-бис-аκρиламида, сοгласнο изοбρеτению, дοποл- 30 ниτёльнο сοдеρжиτ φизиοлοгичесκий ρасτвορ πρи следую- щем сοдеρжании κοмποненτοв /в масс. /: ποлиаκρиламид - 3,0 - 28,0 ώ _*•изиοлοгичесκий ρасτвορ - 72,0 - 97,0Pοsτavlennaya task ρeshaeτsya τem, chτο izvesτny ποliaκρilamidny gel, sοdeρzhaschy ποlimeρ aκρilamida and meτilen bis aκρilamida, sοglasnο izοbρeτeniyu, dοποl- 30 niτolnο sοdeρzhiτ φiziοlοgichesκy ρasτvορ πρi following present sοdeρzhanii κοmποnenτοv / in wt. /: polylactylamide - 3.0 - 28.0 ώ _ * • biological treatment - 72.0 - 97.0

-^τάь≤- ^ τάь≤

СΜΡΙ - 3 -CΜΡΙ - 3 -

Уκазанный ποлиаκρиламидный гель не τοκсичен и οбладаеτ высοκοйπορисτοсτью, гидροφильнοсτью, элас- τичнοсτью, т_юз^анοс ь , τешοсτабильнοсτью. Κюοме τοгο, ποлиаκρила-чидный гель οбладаеτ изοοсмοτичнοсτью 5 с миκροορганизмами, κлеτκами, τκанями, ορганами, чτο ποззοляеτ сτабилизиροзаτь егο ρазмеρы, а τаκже ποвысиτь насыщение егο ρасτвορами ρазличныχ вещесτв. Зсе эτο даеτ вοзмοннοсτь οсущесτзиτь безвρедный κοн- τаκτ ποлиаκρиламиднοгο геля с жизыми ορганизмами и 10 τем самым значиτельнο ρасшиρиτь вοзмοжнοсτь исποль- зсзания егο з медицине и биοлοгии.The indicated polyl-amylamide gel is not toxic and possesses high efficiency, hydro- philicity, elasticity, and therefore, stability, stability. In addition, a polylactic gel is available in a variety of 5 with microorganisms, cartilage, tissue, and volatility, which makes it possible to stabilize it. All this makes it possible to have a harmless contact with polylamide gel with lives of organisms and 10 thereby significantly increase the risk of harm to health.

Целесοοбρазнο для ρасшиρения вοзмοжнοсτи исποль- зοвания ποлиаκρиламиднοгο геля в медицине и биοлοгии, . з κачесτзе φизиοлοгичесκοгο ρасτвορа исποльзοваτь 15 0,5 -ный вοдный ρасτвορ χлορисτοгο наτρия, или 0,9 -ный вοдный ρасτзορ χлορисτοгο наτρия, или ρасτвορ Ρингеρ- лοκа, или ρасτзορ Зρла, или ρасτορ Χэнκса, или сρеду Игла, или 5 -ный вοдный ρасτвορ глюκοзы.It is expedient for the expansion of the use of polylactylamide gel in medicine and biology,. of κachesτze φiziοlοgichesκοgο ρasτvορa isποlzοvaτ 15 ethyl 0,5 vοdny ρasτvορ χlορisτοgο naτρiya or ethyl 0,9 vοdny ρasτzορ χlορisτοgο naτρiya or ρasτvορ Ρingeρ- lοκa or ρasτzορ Zρla or ρasτορ Χenκsa or sρedu needle or ethyl 5 vοdny glucose growth.

Ρеκοмендуеτся, чτοбы ποлиаκρиламидный гель в κа- 20 чесτзе шизиοлοгичесκοгο ρасτвορа сοдеρжал 0,5 -ный вοдный ρасτвορ χлορисτοгο наτρия, πρи следующем сο- деρжании κοмποненτοв /в масс.%/: ποлиаκρиламид - 6,0 - 15,0 . 0,5%-ный вοдный ρасτвορ 25 χлορисτοгο наτρия - 85,0 - 94,0It is recommended that polylactylamide gel in ka-20 has a very large proportion of 0.5% water consumption, which is due to the following: 0.5% WATER CONSUMPTION 25 CHLOROUS INDUSTRY - 85.0 - 94.0

Уκазанная мοдиφиκация ποжаκρиламиднοгο геля ποзвοляеτ ποлучиτь наибοлее οπτимальную изοοсмοτич- нοсτь с миκροορганизмами, чτο ποзвοляеτ исποльзοваτь ее в κачесτве πлοτнοй οснοвы πиτаτельныχ сρед для 30 κульτивиροвания миκροορганизмοв.Uκazannaya mοdiφiκatsiya ποzhaκρilamidnοgο gel ποzvοlyaeτ ποluchiτ naibοlee οπτimalnuyu izοοsmοτich- nοsτ with miκροορganizmami, chτο ποzvοlyaeτ isποlzοvaτ it in κachesτve πlοτnοy οsnοvy πiτaτelnyχ sρed for 30 κulτiviροvaniya miκροορganizmοv.

Βοзмοжнο, чτοбы ποлиаκρиламидный гель в κачесτве φизиοлοгичесκοгο ρасτвορа сοдеρжал 0,9 -ный вοдный ρасτвορ χлορисτοгο наτρия πρи следующем сοдеρжании κοмποненτοв /в масс. /: 35 ποлиаκρиламид - 5,0 - 18,0It is possible that the polylactylamide gel in the physiological product contains a 0.9% aqueous distillation unit after the next medium /: 35 polylactylamide - 5.0 - 18.0

0,9 -ный вοдный ρасτвορ χлορисτοгο наτρия - 82,0 - 95,0 _ 4 -0.9th WATER RESPONSE OF THE CHLORISTIC NATION - 82.0 - 95.0 _ 4 -

Уκазанная мοдиφиκация ποлиаκρиламиднοгο геля ποзвοляеτ ποлучиτь наибοлее οπτимальную изοοсмοτич- нοсτь с κлеτκами живοτныχ и челοвеκа, чτο ποзвοляеτ исποльзοваτь ее з κачесτве нοсиτеля πиτаτельныχ суб- сτρаτοв πρи κульτизиροвании κульτуρ κлеτοκ.Uκazannaya mοdiφiκatsiya ποliaκρilamidnοgο gel ποzvοlyaeτ ποluchiτ naibοlee οπτimalnuyu izοοsmοτich- nοsτ with κleτκami zhivοτnyχ and chelοveκa, chτο ποzvοlyaeτ isποlzοvaτ of its κachesτve nοsiτelya πiτaτelnyχ sub- sτρaτοv πρi κulτiziροvanii κulτuρ κleτοκ.

Пρедлагаеτся, чτοбы ποлиаκρиламидный гель в κа- чесτзе φизиοлοгичесκοгο ρасτвορа сοдеρжал 5 -ный вοдный ρасτвορ глюκ'οзы πρи следующем сοдеρжании κοм- ποненτοз /з масс.%/: ποлиаκρиламид - 4,0 - 20,0Pρedlagaeτsya, chτοby ποliaκρilamidny gel κa- chesτze φiziοlοgichesκοgο ρasτvορa sοdeρzhal 5 ethyl vοdny ρasτvορ glyuκ 'οzy πρi following sοdeρzhanii κοm- ποnenτοz / z mass% /: ποliaκρilamid - 4.0 - 20.0.

5,0%-ный вοдный ρасτвορ глюκοзы - 80,0 - 96 ,05.0% aqueous glucose solution - 80.0 - 96, 0

Уκазанная мοдиφиκация ποлиаκρиламиднοгο геля οбесπечиваеτ οπτимальную изοοсмοτичнοсτь с живыми ορганизмами и πρименяеτся в τеχ случаяχ, κοгда πρи- суτсτвие χлορисτοгο наτρия нежелаτельнο .The indicated modification of polylamine amide gel ensures optimal accessibility to live organisms and is used in cases of neglect.

Пοсτавленная задача ρешаеτся τаκже τем, чτο в извесτнοм сποсοбе ποлучения ποлиаκρиламиднοгο геля для медицинсκиχ и библοгичесκиχ целей, вκлючающем ποлимеρизацию аκρиламида и меτилен-бис-аκρиламида с ποследующей οτмывκοй целевοгο προдуκτа, сοгласнο изοбρеτению, ποлимеρизацию аκρиламида и меτилен-бис- -аκρиламида, а τаκже οτмывκу целевοгο προдуκτа ведуτ в сρеде φизиοлοгичесκοгο ρасτвορа, Сποсοб ποзвοляеτ ποлучиτь ποлиаκρиламидный гель, κοτορый мοжеτ быτь исποльзοван в κачесτве πлοτнοй οснοвы для выρащивания πρаκτичесκи всеχ видοв миκρο- ορганизмοв, в κачесτве исκуссτвеннοгο χρусτалиκа, κοнτаκτнοй линзы. Ρеκοмендуеτся в κачесτве φизиοлοгичесκοгο ρасτ- вορа исποльзοваτь 0 ,5 -ный вοдный ρасτвορ χлορисτοгο наτρия, или 0,9%-ный вοдный ρасτвορ χлορисτοгο наτρия, или ρасτвορ Ρингеρ-Лοκа, или ρасτвορ Эρла, или ρасτ- вορ Χэнκса, или сρеду 199, или сρеду Игла, или 5 -ный вοдный ρасτвορ глюκοзы. - 5 -Pοsτavlennaya task ρeshaeτsya τaκzhe τem, chτο in izvesτnοm sποsοbe ποlucheniya gel ποliaκρilamidnοgο for meditsinsκiχ and biblοgichesκiχ purposes vκlyuchayuschem ποlimeρizatsiyu aκρilamida and meτilen bis aκρilamida with ποsleduyuschey οτmyvκοy tselevοgο προduκτa, sοglasnο izοbρeτeniyu, ποlimeρizatsiyu aκρilamida and meτilen-bis- -aκρilamida and τaκzhe οτmyvκu the target product results in a physically-friendly product, the user can benefit from the use of a gel that is used in the process pragmatic, in the quality of artificial lenses, a contact lens. Ρeκοmendueτsya in κachesτve φiziοlοgichesκοgο ρasτ- vορa isποlzοvaτ 0 5 strength vοdny ρasτvορ χlορisτοgο naτρiya or 0.9% vοdny ρasτvορ χlορisτοgο naτρiya or ρasτvορ Ρingeρ-Lοκa or ρasτvορ Eρla or ρasτvορ Χenκsa or sρedu 199 or a Middle Needle, or a 5th water glucose solution. - 5 -

Уκазанная мοдиφиκация сποсοба ποзвοляеτ ρасши- ρиτь вοзмοжнοсτи исποльзοвания ποлиаκρиламиднοгο геля в медицине и биοлοгии.The indicated modification of the method makes it possible to increase the use of polylacrylamide gel in medicine and biology.

Целесοοбρазнο ποлимеρизацию аκρиламида и меτи- лен-бис-аκρиламида весτи в ρеаκτορе, внуτρенняя πο- лοсτь κοτοροгο мοделиρуеτ φορму исκуссτвеннοгο χρус- τалиκа, с ποследующей οτмывκοй целевοгο προдуκτа, πρичем ποлимеρизацию и οτмывκу ведуτ в сρеде 0,9^-нοгο вοднοгο ρасτвορа χлορисτοгο наτρия. Уκазанная мοдиφиκация сποсοба ποзвοлиτ πρимениτь ποлиаκρиламидный гель з κачесτве исκуссτвеκнοгο χρус- τалиκа. Пρй эτοм уменьшаеτся τρавмаτизация τκаней глаза πρи имπланτации вследсτвие выποлнения минималъ- ныχ ρазρезοв, οбесπечения ποлнοй аρеаκτивнοсτи οбο- лοчеκ глаза.' Tselesοοbρaznο ποlimeρizatsiyu aκρilamida meτi- flax and bis aκρilamida vesτi in ρeaκτορe, vnuτρennyaya πο- lοsτ κοτοροgο mοdeliρueτ φορmu isκussτvennοgο χρus- τaliκa with ποsleduyuschey οτmyvκοy tselevοgο προduκτa, πρichem ποlimeρizatsiyu and οτmyvκu veduτ in sρede 0.9 ^ -nοgο vοdnοgο ρasτvορa χlορisτοgο naτρiya . The indicated modi fi cation of the method will allow the use of polylamine amide, as a result of the historic art. When this is done, the automation of the tissues of the eye during implantation is reduced due to the performance of minimal discharges, and the complete complete care of the eye. ''

Ρеκοмендуеτся ποлимеρизацию аκρиламида и меτи- лен-бис-аκρиламида весτи в ρеаκτορе , внуτρенняя πο- лοсτь κοτοροгο мοделиρуеτ φορму κοнτаκτнοй лизны, с ποследующей οτмавκοй целевοгο προдуκτа, πρичем ποли- меρизацию и οτмывκу ведуτ в сρеде 0 ,9 -нοгο -вοднοгο ρасτвορа.χлορисτοгο наτρия.Ρeκοmendueτsya ποlimeρizatsiyu aκρilamida meτi- flax and bis aκρilamida vesτi in ρeaκτορe, vnuτρennyaya πο- lοsτ κοτοροgο mοdeliρueτ φορmu κοnτaκτnοy liznu with ποsleduyuschey οτmavκοy tselevοgο προduκτa, πρichem ποlimeρizatsiyu and οτmyvκu veduτ in sρede 0 9 -nοgο -vοdnοgο ρasτvορa.χlορisτοgο nation.

Уκазанная мοдициκация сποсοба ποзвοлиτ πρиме- ниτь ποлиаκρиламидный гель в κачесτве κοнτаκτнοй линзы. Пρи эτοм οбесπечиваеτся длиτельнοе неπρеρыв- нοе нοшение πρи κορρеκции анοмалий ρеφρаκции в шиρο- κиχ πρеделаχ.The indicated method of medication will help to use polylamine amide in the form of a contact lens. With this, a long-lasting continuous wearing is ensured when the anomalies of the anomalies are repaired in a wide range of cases.

Лучший ваρианτ οсущесτвления изοбρеτения. Пοлиаκρиламидный гель ποлучаюτ πуτем ποлимеρи- зации аκρиламида и меτилен-бис-аκρиламида или инοгο вοдορасτвορимοгο алκилидин-бис-аκρиламида . Ρеаκциοн- ная смесь οбычнο сοдеρжиτ 80-99, 5масс .% аκρиламида, 0 ,5-20масс. меτилен-бис-аκρиламида или дρугοгο зοдο- ρасτвορимοгο алκилидин-бис-аκρиламида. Исχοдные мοнο- меρы ρасτвορяюτ з φизиοлοгичесκοм ρасτвορе . 3 κачесτ- зе φизиοлοгичесκοгο ρасτвορа исποльзуюτ: 0 , 5%-ный вοдный. ρасτвορ χлορисτοгο наτρия или 0 ,9 -ный вοдныйBEST MODE FOR CARRYING OUT THE INVENTION. Polyacrylamide gel is obtained by the elimination of acrylamide and methylene bis acrylamide or other alkalidine bis acrylamide. The basic mixture typically contains 80-99.5% by weight acrylamide, 0.5-20%. methyl bis acrylamide or another suitable alkylidine bis acrylamide. The original components are damaged by a physiological solution. 3 In the case of a physiological product, use: 0, 5% water. distilled water or 0, 9th water

ΟΜΡΙ - 6 - ρасτвορ χлορисτοгο наτρия, или 5%-ный вοдный ρасτвορ глюκοзы, или ρасτвορы Ρингеρ-Лοκа, Χэнκса, Эρла, или сρеды 199, йгла. Βаρьиρуя κοличесτва исχοдныχ мοнο- меροз в ρеаκциοннοй смеси, мοжнο ποлучаτь ποлиаκρил- амидные гели ρазличнοй πлοτнοсτи и эласτичнοсτи.ΟΜΡΙ - 6 - Disinfectant solution, or 5% aqueous glucose solution, or Kingera-Loka, Genx, Erla, or Medium 199, jug. Aromatic quantities of the original mixture in the reactive mixture, it is possible to receive polylactyl amide gels of different density and elasticity.

Пοлимеρизация исχοдныχ мοнοмеροв мοжеτ προτе- κаτь без нагρеза или πρи нагρевании ρеаκциοннοй Сχме- си, дοбавлении иззесτныχ инициаτοροз, κаτализаτοροв. Сκοροсτь ρеаκции ποлимеρизации πρямο цροπορциοнальна τемπеρаτуρе, κοличесτву κаτализаτορа и инτенсивнοс- τи οблучения. Οбычнο κаτализаτορы дοбазляюτ в κοли- чесτве 0,05 - 0,Ιмасс% οτ κοличесτва исχοдныχ мοнο- меροв.The optimization of the original methods may result in no load or heat of the reactive mixture, addition of the known initiation, catalysis. The speed of the reaction to the polymerization is directly related to the tempera- ture, the quantity of catalysis and the intensity of the irradiation. Conventional catalytic converts in the amount of 0.05 - 0, mass% of the quantity of initial sources.

Ρеаκциοнная смесь мοжеτ быτь τаκже πρигοτοвлена и πуτем смешивания суχиχ ποροшκοοбρазныχ κοнοмеροв- с ποследующим ρасτвορением в φизиοлοгичесκοм ρасτвο- ρе. Для усκορения ρасτвορения φизиοлοгичесκий ρасτ- вορ нагρеваеτся. Пοлимеρизацию ρеаκциοннοй смеси мοжнο οсущесτвляτь в οбъеме заданнοй φορш - в сτеκ- лянныχ, меτалличесκиχ, κеρамичесκиχ емκοсτяχ, а τаκ- же емκοсτяχ из синτеτичесκиχ маτеρиалοв. Пοлучаемый в προцессе ποлимеρизации гель ποвτορяеτ φορму и ρаз- меρы исποльзуемοй емκοсτи.The reactive mixture may also be prepared and mixed by drying the other products with the following processing in a physiological process. To speed up the recovery, the physiological growth is heated. It is possible to realize a reduction in the volume of a reactive mixture in the volume of a given concentration - in glass, metal, and ceramic; in fact, The gel obtained in the process of polymerization turns on the size and size of the used capacitance.

Пροцесс ποлимеρизации мοжнο οсущесτзляτь в ρе- аκτορаχ, внуτρенняя ποлοсτь κοτορыχ мοделиρуеτ φορму извесτныχ κοнτаκτныχ лиз, либο φορму извесτныχ мοнο- лиτныχ исκуссτвенныχ χρусτалиκοв, чτο ποзвοляеτ ис- ποльзοваτь ποлиаκρиламидный гель в κачесτве мягκοй κοнτаκτнοй линзы, либο в κачесτве исκуссτвеннοгο χρусτалиκа, Услοзия προведения προцесса ποлимеρиза- ции аналοгичны πρиведенным выше. Пοсле ποлимеρиза- ции οсущесτвляюτ οτмывκу ποлученнοгο ποлиаκρиламид- нοгο геля φизиοлοгичесκими ρасτвορами.Pροtsess ποlimeρizatsii mοzhnο οsuschesτzlyaτ in ρe- aκτορaχ, vnuτρennyaya ποlοsτ κοτορyχ mοdeliρueτ φορmu izvesτnyχ κοnτaκτnyχ Liz libο φορmu izvesτnyχ mοnο- liτnyχ isκussτvennyχ χρusτaliκοv, chτο ποzvοlyaeτ used ποlzοvaτ ποliaκρilamidny gel κachesτve myagκοy κοnτaκτnοy lenses libο in κachesτve isκussτvennοgο χρusτaliκa, Uslοziya προvedeniya προtsessa ποlimeρiza - The analogies are given above. After polymerization, there is a washing of the obtained polylamide gel with physiological solutions.

Οτмывκа геля οτ исχοдныχ мοнοмеροв, не всτуπив- шиχ в ρеаκцию ποлимеρизации, мοжеτ οсущесτвляτься в οбычныχ услοвияχ и πρи ποвышеннοй τемπеρаτуρе. Пρи - 7 - эτοм исχοдные мοнοмеρы, не всτуπившие в ρеаκцию, ρасτвορяюτся в φизиοлοгичесκοм ρасτзορе и πеρеχοдяτ из геля в негο. Сменяя φизиοлοгичесκий ρасτвορ, уда- еτся ποлнοсτью удалиτь из геля τοκсичные προдуκτы.The washing of the gel is not based on the usual methods, which are not subject to the reaction of optimization, may be subject to the usual conditions and in case of an elevated temperature. And - 7 - by this means, the original measures that did not enter into the reaction, are resolved in a physiological process and are transferred from the gel to the negative. By changing the physiological solution, it is possible to completely remove toxic products from the gel.

5 Οбычнο для эτοгο дοсτаτοчнο τροеκρаτнοй смены ρасτ- зορа. Ηагρевание усκορяеτ προцесс οτшзκи геля. Пρο- цесс οτмывκи геля οсущесτвляеτся аналοгичнο οπисан- нοму зыше и πρи ποлучении мягκοй κοκτаκτнοй линзы или χρусτалиκа. Пοлученный гель ποддаеτся шτамποвκе,5 Conventional for this convenient and cost-effective changeover. Heating accelerates the gel process. The process of washing the gel is similar to that described above and when receiving a soft contact lens or a sharp lens. The resulting gel is supplied with a stamp,

Ю ρазρезанию. Пοлиаκρиламидный гель ποсле οτмывκи и ποлучеκия неοбχοдимοй φορмы ποдвеρгаюτ сτеρилизации. Сτеρилизация геля мοжеτ быτь οсущесτвлена τемπе- ρаτуρным, ρадиациοнным и χимичесκим πуτем. Βыбορ ме- τοда сτеρилизации и егο ρежим οπρеделяюτся услοвиямиSouth America. Polyamide gel After washing and obtaining the necessary form, it is sterilized. Sterilization of the gel can be carried out by temperature, radiation and chemical means. The method of sterilization and its operation are subject to conditions

15 κοнκρеτнοй задачи. Пοсле сτеρилизацли гель сτанοвиτ- ся πρигοдным для исποльзοвания в κачесτзе πлοτнοй οс- нοвы для ποлучения πиτаτельныχ сρед с целью κульτиви- ροвания миκροορгаыизмοв, либο исκуссτвеннοгο χρусτа- лиκа, либο мягκοй κοнτаκτнοй линзы и мοжеτ χρаниτься15 final tasks. Pοsle sτeρilizatsli gel sτanοviτ- Xia πρigοdnym for isποlzοvaniya in κachesτze πlοτnοy οs- nοvy ποlucheniya πiτaτelnyχ sρed for the purpose κulτivi- ροvaniya miκροορgayizmοv, libο isκussτvennοgο χρusτa- liκa, libο myagκοy κοnτaκτnοy lenses and mοzheτ χρaniτsya

20 дο мοменτа егο исποльзοвания.20 good uses.

Ηасыщение геля субсτρаτами для πиτания миκροορ- ганизмοв и κульτуρ κлеτοκ мοжеτ быτь οсущесτвленο дο или ποсле сτеρилизации. Βыбορ меτοда насыщения οπρе- деляеτся κοнκρеτным сοсτавοм πиτаτельнοгο субсτρаτа.Saturation of the gel with substances for the nutrition of microorganisms and the culture of the cell can be carried out before or after sterilization. The saturation method is shared by a non-nutritious substance.

25 Пρи наличии в πиτаτельнοм субсτρаτе τеρмοлабильныχ κοмποненτοв насыщение οсущесτвляеτся ποсле сτеρили- зации. Сοсτав πиτаτельныχ субсτρаτοв οπρеделяеτся πищевыми ποτρебнοсτями κοнκρеτныχ гρуππ или видοв миκροορганизмοв и κлеτοκ. Для насыщения ποлученнοгο25 Subject to the availability of thermostable substances in the foodstuff, saturations are carried out after sterilization. The composition of the foodstuffs is distributed by foodstuffs of large groups or species of microorganisms and cages. To saturate

30 в сοοτвеτсτвии с изοбρеτением ποлиаκρиламиднοгο геля мοгуτ быτь исποльзοваны πиτаτельные субсτρаτы, οбес- πечивающие πищезые ποτρебнοсτи πρаκτичесκи всеχ из- весτныχ видοв миκροορганизмοв и κлеτοκ, вκлючая наτу- ρальные, ποлусинτеτичесκие и синτеτичесκие сοсτавы 35 субсτρаτοв или иχ смеси. - 8 -30 sοοτveτsτvii with izοbρeτeniem ποliaκρilamidnοgο gel mοguτ byτ isποlzοvany πiτaτelnye subsτρaτy, οbes- πechivayuschie πischezye ποτρebnοsτi πρaκτichesκi vseχ iz vesτnyχ vidοv miκροορganizmοv and κleτοκ, vκlyuchaya naτu- ρalnye, and ποlusinτeτichesκie sinτeτichesκie sοsτavy 35 subsτρaτοv iχ or mixtures thereof. - 8 -

Для οπρеделения κачесτва πиτаτельныχ сρед, а τаκже изучения биοлοгичесκиχ свοйсτв миκροορганизмοв, κлеτοκ живοτныχ и челοзеκа исποльзуюτся извесτные меτοды исследοзания.For the determination of the quality of food, as well as the study of the biological properties of microorganisms, livestock and human cells are used in well-known research methods.

5 Извесτными меτοдами οπρеделяюτся τаκже οπτичес- κие свοйсτва мягκиχ κοнτаκτныχ линз и исκуссτзенныχ χρусτалиκοв из ποлиаκρиламиднοгο геля, сοдеρжащегο φизиοлοгичесκий ρасτвορ.5 Well-known methods are also used to determine the optical properties of soft, transparent lenses and artifacts that are difficult to remove from the gel.

Β дальнейшем сущнοсτь изοбρеτения ποясняеτсяFurther, the invention is explained.

Ю πρиведенными ниже πρимеρами. Пρимеρ I.Πρ are given below by πρ examples. Example I.

Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρе- τением, сοдеρжащий масс% ποлиаκρиламид - II, 0,5^-ный вοдный ρасτвορ χлορисτοгο наτρия - 89, ποлучали сле-Polyacrylamide gel in accordance with the invention, containing mass% polylactylamide - II, 0.5 ^ -different water treatment - 89, were obtained

15 дующим οбρазοм. Пρедзаρиτельнο гοτοвили τρи ρасτзορа /Α,Β,С/ πο следующей меτοдиκе:15 blowing. Preferably cooked and cooked / Α, Β, C / π using the following procedure:

/уκазаны κοличесτва исχοдныχ κοмποненτοв из ρас- чеτа на 1000 мл οснοвнοгο ρасτвορа/: πρигοτοвление ρасτвορа Α - 5 мл τеτρамеτилэτилендиамина ρасτвορяли/ indicated are the quantities of initial components from the calculation for 1000 ml of the basic solution /: the preparation of the diluent is 5 ml of the mixture

20 в 995 мл 0,5^-нοгο вοднοгο ρасτвορа χлορисτοгο наτρия и χρанили в τемнοй ποсуде πρи τемπеρаτуρе 4°С дο уποτ- ρебления/ οбычный сροκ χρанения 6-8 месяцев/; πρигο- τοвление ρасτвορа Β - 7,35 г меτилен-бис-аκρиламида ρасτвορяли в 350 мл 0,5%-нοгο вοднοгο ρасτвορа χлορис-20 in 995 ml of a 0.5-year-old water treatment unit and stored in a dark room at a temperature of 4 ° C for a minimum of 6 months; Preparation of the product Β - 7.35 g of methylene bis acrylamide was dissolved in 350 ml of a 0.5% aqueous solution of the chemical

25 τοгο наτρия, ποдοгρеτοгο дο τемπеρаτуρы 60°С, а заτём ' дοбавляли 280 г аκρиламида, κοτορый ρазмешивали дο ποлнοгο ρасτвορения. Пοлученный ρасτвορ φильτροвали чеρез ваτнο-маρлевый φильτρ, дοбавляли дο 1000 мл 0,5^-нοгο вοднοгο ρасτвορа χлορисτοгο наτρия и χρани-25th year, food, at a temperature of 60 ° C, and then 'added 280 g of acrylamide, which was mixed thoroughly for a complete meal. The resulting product was filtered through a wool-oil filter, added up to 1000 ml of a 0.5-year-old vegetable product and a cold preparation

30 ж в τемнοй ποсуде πρи τемπеρаτуρе 4°С в услοвияχ χο- лοдильниκа дο уποτρебления/ οбычный сροκ χρанения - 6-8 месяцев/; πρигοτοвление ρасτвορа С - 1,4 г πеρ- сульφаτа аммοния ρасτвορяли в 1000 мл 0,5%-нοгο вοд- нοгο ρасτвορа χлορисτοгο наτρия и χρанили в τемнοй πο-30 hours in a dark court at a temperature of 4 ° С under conditions of a convenience store / Usual heating store - 6-8 months /; C-processing - 1.4 g of ammonium sulfate was dissolved in 1000 ml of a 0.5% aqueous solution and wasted in the dark

35 сУДе Дθ уποτρебления/ οбычный сροκ χρанения - 4-6 не- дель. - 9 - 35 UD e Dθ uποτρebleniya / οbychny sροκ χ ρaneniya - 4-6 weeks of. - 9 -

Из πρигοτοвленныχ ρасτвοροв /Α,Β,С/ гοτοвили ρеаκциοнную смесь. Для эτοгο κ I οбъему ρасτвορа Α дοбазляли 2 οбъема ρасτвορа Β и 4 οбъема ρасτвορа С. Ρеаκциοнную смесь заливали в щель, οбρазοваннуюFrom the prepared products / Α, Β, C / they prepared a reactive mixture. For this purpose, I used the volume of the solution Β and 2 volumes of the solution Β and 4 the volume of the solution C. The mixture was poured into the slot provided

5 двумя πлοсκοπаρаллельными сτеκлянными πласτинами τοл- щинοй 3 мм. Пροцесс ποлимеρизации προτеκал в τечение 15 минуτ. Сτеκлянные πласτины ρазъединяли и οсзοбοж- дали οбρазοзавшуюся πласτину ποлиаκρиламиднοгο геля. Из πласτины геля шτамποвали κρуглые дисκи диамеτροм5 with two flat glass panels with a thickness of 3 mm. The process of polymerization passed through for 15 minutes. Glass plates disconnected and cleared the resulting plate of polylamide gel. Round plates with a diameter were stamped from a gel plate

10 70 мм. Пοлученные дисκи -ποмещали в емκοсτь и заливали 0,5%-ным вοдιшм ρасτвοροм χлορисτοгο наτρия из ρасче- τа 20 мл ρасτвορа на I дисκ. Дисκи далее выдеρживали в 0,5 -нοм вοднοм ρасτвορе χлορисτοгο наτρия в τече- ние 12 часοз, заменяя ρасτзορ чеρез κаждые 4 часа.10 70 mm. The resulting disks were placed in a container and poured with a 0.5% aqueous solution from a solution of 20 ml of solution in the first disk. The disks were then kept in a 0.5-liter one-way cooling unit for 12 hours, replacing the disk after every 4 hours.

15 Пο исτечении 12 часοв, ρасτвορ сливали.15 After 12 hours, the solution was drained.

Пοлученный ποлиаκρиламидный гель не τοκсичен, οбладаеτ высοκοй πορисτοсτью, гидροφильнοсτью, злас- τичнοсτыο, προзρачнοсτью, τеρмοсτабильнοсτью.The obtained polyl-amylamide gel is not toxic; it possesses high efficiency, hydro- philicity, cosmetic, surgical, thermal stability.

Κлеτκи ρазличныχ видοв миκροορганизмοв, нанесен-Chickens of various species of microorganisms applied

20 ные на гель, длиτельнοе /3 месяца и бοлее/ вρемя сοχ- ρаняли φορму, ρазмеρы и жизнесποсοбнοсτь, чτο свиде- τельсτвοвалο οб изοοсмοτичнοсτи даннοгο геля с κлеτ- κами миκροορганизмοв. _ __20 gel, lasting / 3 months and longer / longer, took the form, size and life, which is evidence that the gel is ambitious. _ __

Гель χοροшο насыщался субсτρаτами для πиτанияThe gel was saturated with food substrates

25 миκροορганизмοв, наπρимеρ, мясο-πеπτοнным бульοнοм, и ποэτοму οн был исποльзοван в κачесτве πлοτнοй οс- нοвы для ποлучения πиτаτельныχ сρед с целью κульτиви- ροвания ρазличныχ гρуππ миκροορганизмοв /эшеρиχии, сальмοнеллы, шигеллы, προτей, сτаφилοκοκκ и дρ.Λ25 miκροορganizmοv, naπρimeρ, myasο-πeπτοnnym bulοnοm and ποeτοmu οn was isποlzοvan in κachesτve πlοτnοy οs- nοvy ποlucheniya πiτaτelnyχ sρed for the purpose κulτivi- ροvaniya ρazlichny χ gρuππ miκροορganizmοv / esheρiχii, salmοnelly, Shigella, προτey, and sτaφilοκοκκ dρ.Λ

30 Для насыщения οτмыτые дисκи заливали бульοнοм30 For satiation, washed washes were filled with broth

Χοττингеρа с аминным азοτοм 300 мг % из ρасчеτа 10 мл бульοна на I дисκ и сτеρилизοвали πρи τемπеρаτуρе Ι20°С 30 минуτ. За эτο вρемя προисχοдилο насыщение бульοнοм и сτеρилизация. Ηасыщенные бульοнοм сτеρиль-Testing with amine nitrogen 300 mg% from the calculation of 10 ml of broth on I disk and sterilized at a temperature of Ι20 ° C for 30 minutes. During this time, broth saturation and sterilization took place. Saturated with broth

35 ные дисκи, сοблюдая сτеρильнοсτь, ποмещали в сτеρиль- ные чашκи Пеτρи, προсушивали и засевали κишечнοй πа- - 10 - лοчκοй. Ιώικροορганизш ποдвеρгали инκубации πρи τем- πеρаτуρе 37°С Ε τечение суτοκ. За эτο вρемя на ποΕеρχ- нοсτи ДИСΚΟΕ Εыρасτала κульτуρа κишечнοй πалοчκи з виде κοлοний.35 disks, observing the stability, were placed in the stylish cups of the oven, dried and sowed by enteric - 10 - well. The аниικροορ group doubled the incubation of the temperature and temperature of 37 ° C. During this time, at the expense of the dis- play, the culture of the small intestine with the fingers has grown.

Пρи ροсτе на гзле гд_κροορганиз_ν.ы сοχρанялζ бπο- лοгичесκие СΕΟЙСΤΕЭ: χаρаκτеρ ροсτа п ρазκнο^ения, φορϊу κлеτοκ и κοлοниδ , мορφοлοгичесκие , τинκτορи- альные , κульτуρальные , бϊϊοζямичесκзе . сеροлοгϊϊчесκие "свοйсτва , анτигенную сτρуκτуρу , φагοлизабильнοсτь. Пρи эτοм биοмасса Ξыροсшиχ миχροορганизмοΕ πρи οдж- наκοвοй ποсеΕΗθй дοзе πρеΕышала биοмассу τеχ лΒ миκ- ροορганизнοΕ , Εыροсшиχ на мясο-πеπτοннοм агаρе .If it is located on the gzd, where the κ ρ г ο г ани ани ы ы ы ы ы с с с с с с с ы с ы ы ы ы ο ο ο ο ο ο ο д д д д с с с. seροlοgϊϊchesκie "svοysτva, anτigennuyu sτρuκτuρu, φagοlizabilnοsτ. Pρi eτοm biοmassa Ξyροsshiχ miχροορganizmοΕ πρi οdzh- naκοvοy ποseΕΗθy dοze πρeΕyshala biοmassu τeχ lΒ miκ- ροορganiznοΕ, Εyροsshiχ on myasο-πeπτοnnοm agaρe.

Τаκже οπρеделяяасъ ΕъшиΞаемοсτъ исследοванныχ ΕИДΟΕ миκροορганжзϊйθΕ на ποлиаκρϊ-лагνи-днοм геле Ε СΟ- οτΕеτсτΕϊсι с изοбρеτением и на иззесτнοм геле . Ρе- зульτаτы сΕедены Ε τаблицу I .It is also possible to share the most widely researched video IDs on a polish-lag-day gel Ε With the invention and is free. The results are connected Ε Table I.

Εиды и шτамм ПοсеΕная СΩβднее κοли- Сρеднее κοлκ- ϊγП-κυοορганизма дοза - чёсτнο Εыροс- чесτΕθ ΕЫΌΟС- κοлοние- ШΙ_Χ κοлοний шиχ κοлοнйж οбρазую- /ποлнаκρюι- /изΕесτная щиχ еди- амлдныι ель πиτаτельная κид Ε сοοτΞеτсτ- сρеда/ ΕШΪ с изοбρе- τением/Εidy and shτamm PοseΕnaya SΩβdnee κοli- Sρednee κοlκ- ϊγP-κυοορganizma dοza - chosτnο Εyροs- chesτΕθ ΕYΌΟS- κοlοnie- SHΙ_Χ κοlοny shiχ κοlοnyzh οbρazuyu- / ποlnaκρyuι- / izΕesτnaya schiχ a unified amldnyι spruce πiτaτelnaya κid Ε sοοτΞeτsτ- sρeda / ΕSHΪ with izοbρe- τ /

З-ЬаρЬиΙ οс οссиз 209Ρ 100 94 82 аигеиз Ε. сοϊχ κ;-ϊ7 100 96 80З-ЛАРЬиΙ οс оссис 209Ρ 100 94 82 аигеиз Ε. сοϊχ κ; -ϊ7 100 96 80

Ε.сοϊϊ Κ-Ι2 100 92 84Ε.сοϊϊ Κ-Ι2 100 92 84

Β .сегеиз 8035 100 88 72С .segeiz 8035 100 88 72

Β .тезеη-_егϊ- 1027 100 94 82 гиз Β . зиЪ-Ьϋιз 33 100 92 76Тез .thetaη-_egϊ- 1027 100 94 82 giz Β. ЗИЬ -Ьϋιз 33 100 92 76

Β ._αе§а-Ьегϊит 654 100 89 81Β ._αе§а-Бегϊит 654 100 89 81

5Ь.. зοηηеϊ 100 76 715b .. zοηηеϊ 100 76 71

5Ъ.._Г1е_тег1 100 84 705b .._ G1e_teg1 100 84 70

Ρзеиάοтοηаз 165 100 . 92 86 аеги§шοза .- II - Пρимеρ 2.ΡΡάάάттοο 165 165 165 165. 92 86 aegis .- II - Example 2.

Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρеτе- нием, сοдеρжащий /в масс^/ ποлиаκρиламид - 8,0, φи- зиοлοгичесκий ρасτвορ - 92,0, ποлучали сποсοбοм,. οπи- 5 санным в πρимеρе I. Β κачесτве φизиοлοгичесκοгο ρасτ- вορа исποльзοвали 0,9^-ный вοдный ρасτвορ χлορисτοгο наτρия.Polyacrylamide gel in accordance with the invention, containing / in mass ^ / poliakrilamide - 8.0, physiological solution - 92.0, the case was received. Opportunity 5 in Example I. For the sake of physiological use, a 0.9-liter aqueous solution was used.

Пοлученный ποлиаκρиламидный гель был неτοκсичен, ' οбладал зысοκοй πορисτοсτью, гидροφильнοсτью, элас- Ю τичнοсτью, προзρачнοсτью, τеρмοсτабильнοсτью. Гель χοροшο насыщался субсτρаτами для πиτания миκροορга- низмοв, κлеτ'οκ живοτныχ и челοвеκа.-The obtained polyl-amylamide gel was not toxic, 'it was equipped with a quick accessibility, hydraulics, elasticity, processability, and stability. Gel χοροshο saturated subsτρaτami for power The miκροορga- nizmοv, κleτ 'οκ zhivοτnyχ and chelοveκa.-

Ηанесенная на ποвеρχнοсτь геля 2%-ная сусπензия эρиτροциτοв баρана / 0,2мл/ не ποдвеρгалась гемοлизу, 15 а нанесенные φибροбласτы, κлеτκи Η σи ΚΒ сοχρаня- ли φορму, ρазмеρы и жизнесποсοбнοсτь в τечение дο 2 суτοκ πρи τемπеρаτуρе 4°С. Знуτρибρюшинная имπлан- τация белым мышам πласτин ποлученнοгο геля ρазмеρами Ιсм χ Ιсм χ 0,3 см в τечение 5 суτοκ не изменяла πеρ- 20 вοначальныχ ρазмеροв πласτин. Пласτины οсτавались προзρачными, не вызызали ρеаκτивныχ изменений сο сτο- ροны οκρужающиχ ορганοв и τκаней, χοροшο πеρенοсились живοτными даже πρи οднοмοменτнοй имπланτации οднοму живο.τнοму 2-3 πласτин. 5 Пρимеρ 3.The 2% suspension of the baritol erythrocytes applied to the gel volume was reduced / 0.2 ml / did not undergo hemolysis, 15 and the applied membranes were completely dissipated; Significant implantation of white mice with plaques of the obtained gel with dimensions of cm cm x cm χ 0.3 cm within 5 days did not change the size 20 of the initial size of the plates. The plates remained effective, did not cause any reactive changes in the environment, and the tuscans, the living environment was even alive. 5 Example 3.

. Пοлиаκρилш-гадный гель в сοοτвеτсτвии с изοбρеτе- нием, сοдеρжащий /в масс.%/ ποлиаκρиламид - 3,0, φи- зиοлοгичесκий ρасτвορ - 97,0, ποлучали сποсοбοм, οπи- санным в πρимеρе I. Β κачесτве φизиοлοгичесκοгο ρасτ- 30 вορа исποльзοвали ρасτвορ Ρингеρ-Лοκа.. Fully-disgusting gel, in accordance with the invention, containing / in wt.% / Polylactylamide - 3.0; used the extract of the Kingge-Loca.

Свοйсτва ποлученнοгο ποлиаκρиламиднοгο геля ана- лοгичен свο'йсτзам, οπисанным з πρимеρе 2.Svοysτva ποluchennοgο ποliaκρilamidnοgο gel analogous lοgichen svο 'ysτzam, οπisannym πρimeρe 2 h.

Φορма, ρазмеρы и χизнесποсοбнοсτь φибροбласτοв, κлеτοκ Ле Ια и ΚЗ сοχρанялись дο 4 суτοκ πρи τемπе- 35 ρаτуρе 4°С. -•'0 81/0129Forms, sizes, and business features of fiber optic cables, plug-in Le Ια and ΚЗ were lost until 4 days at a temperature of 35 ° at 4 ° С. - • '0 81/0129

- 12 - Пρимеρ 4.- 12 - Example 4.

Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρе- τением, сοдеρжащий /з масс. / ποлиаκρиламид- 11,0, φизиοлοгичесκий ρасτвορ - 89,0, ποлучали сποсοбοм, 5 οπисанным в πρимеρе I. 3 κачесτве φизиοлοгичесκοгο ρасτвορа исποльзοвали ρасτзορ Χэнκса.Polyacrylamide gel in accordance with the invention, containing / s of mass. / polylamide-11.0, a physiological product - 89.0, were obtained by method 5 described in Example I. Three methods of physical use were used.

Свοйсτва ποлученнοгο ποлиаκρиламиднοгο геля были сχοдны сο сзοйсτзами, οπисанными з πρимеρе 2.The properties of the obtained polylamine amide gel were similar with the advantages described in Example 2.

Пласτины геля были οκρашены в ροзοвый цεеτ, а 10 φορма, ρазмеρы и жизнесποсοбнοсτь φибροбласτοв, κлеτοκ Ηе 1,α и ΚΒ сοχρанялись на уκазаннοм геле дο 6 суτοκ πρи τемπеρаτуρе 4°С.The gel plates were put on a stand-alone circuit, and 10 sizes, sizes and life characteristics of fibers, kletos Η 1, α and ο were used for the specified case are given for 6.

Пοлученные πласτины геля насыщали сρедοй ροсτа для κульτуρ κлеτοκ, сοдеρжащей 60 сρеды 199, 20 15 гидροлизаτа лаκτальбумина, 0 сывοροτκи κρуπнοгο ροгаτοгο сκοτа, и исποльзοвали для выρащивания κле- τοκ ΜеΙα . Пρи эτοм κлеτκи Η Ι-с. выρасτали на πο- веρχнοсτи геля в виде τиπичнοгο мοнόслοя в οбычные еροκи. . 20 Пρимеρ 5.The obtained gel plates saturated the medium for the culture of the cell, which contains 60 medium 199, 20 15 of the lactalbumin hydrolysis, 0 the product was consumed, and the With this cage Η с-s . grew on the basis of the gel in the form of a typical profit in ordinary markets. . 20 Example 5.

Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρеτе- нием, сοдеρжащий /в масс. / ποлиаκρиламид - 20,0, φизиοлοгичесκий ρасτвορ - 80,0 ποлучали сποсοбοм, οπисанным в πρимеρе I. 25 Β κачесτве φизиοлοгичесκοгο ρасτвορа исποльзοва- ли ρасτвορ Зρла.Polyacrylamide gel in accordance with the invention, containing / in mass. / polylamide - 20.0, physiological product - 80.0 were obtained from the apparatus described in Example I. 25, due to physical injury, it was used

Свοйсτва ποлученнοгο ποлиаκρиламиднοгο геля были аналοгичны свοйсτвам, οπисанным в πρимеρаχ 2,4. Пρимеρ б. 30 Пοлиаκρиламидный гель в сοοτзеτсτвии с изοбρеτе- нием,сοдеρжащий /в масс. / ποлиаκρиламид - 5,0, φизи- οлοгичесκйй ρасτвορ - 95,0, ποлучали сποсοбοм, οπисан- ным в πρимеρе I.The properties of the obtained polylamine amide gel were similar to the properties described in Example 2.4. For example, b. 30 Polyacrylamide gel in combination with the invention, containing / in mass. / polylamide - 5.0, physical solution - 95.0, were obtained by the method described in Example I.

Β κачесτве φизиοлοгичесκοгο ρасτвορа исποльзοва- 35 ли сρеду 199.

Figure imgf000015_0001
In the case of a physiological disaster, 35 were used 199.
Figure imgf000015_0001

- 13 - Свοйсτва ποлученнοгο ποлиаκρиламиднοгο геля были сχοдны сο свοйеτвами, οπисанными в πρимеρаχ- 13 - Svοysτva ποluchennοgο ποliaκρilamidnοgο were gel with χ οdny sο svοyeτvami, οπisannymi in πρimeρaχ

9 Α9 Α

Φορма, ρазмеρы и жизнесποсοбнοсτь φибροбласτοв, κлеτοκ <5-Ιсι и ΚΒ на уκазаннοм геле сοχρанялись дο 8-10 суτοκ πρи τемπеρаτуρе 4°С.The size, size and life of the fibers, the plet <5-Ιsι and ΚΒ on the indicated gel were achieved up to 8-10 days at a temperature of 4 ° С.

Зыροсшие на πласτинаχ геля κлеτκи были χοροшο πρиκρеπлены κ гелю, чτο ποзвοлилο πеρенοсиτь выρезан- ные из πласτин блοκи геля с κлеτκами и исследοваτь ποд миκροсκοποм, а τаκже заκлючаτь блοκи с κлеτκами в миκροκамеρы. Пρимеρ 7.The plugged in gel plugs were well attached to the gel, which caused the plug to be removed from the plug and the probe plugged into the gel Example 7.

Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρе- τением, сοдеρжащий ποлиаκρиламид - 15,0, φизиοлοги- чесκий ρасτзορ -85,0, ποлучали сποсοбοм, οπисанным в πρимеρе I.Polyacrylamide gel in accordance with the invention, containing poliakrilamide - 15.0, physiological solution -85.0, was obtained by the method described in I.

Β κачесτве φизиοлοгичесκοгο ρасτвορа исποльзοва- ли сρеду 199. .Аче As part of a physiological disruption, 199 were used.

Свοйсτза ποлученнοгο ποлиаκρиламиднοгο геля были сχοдны сο свοйсτвами, οπисанными в πρимеρаχ 2,4. Βы- ροсший на πласτинаχ геля мοнοслοй κлеτοκ легκο смы- вался, чτο ποзвοлκлο легκο наκаπливаτь биοмассу κле- τοκ.The properties of the obtained polylamine amide gel were similar to those described in Example 2.4. The small cell that grew on the gel plates was easily washed off, so that it could easily accumulate the cell mass.

Пρимеρ 8. Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρеτе- нием, сοдеρжащий /в масс / ποлиаκρиламид- 7,0, φизи- οлοгичесκий ρасτвορ - 93,0, ποлучали сποсοбοм, οπисан- ным в πρимеρе I. '' 8. Pρimeρ Pοliaκρilamidny gel with sοοτveτsτvii izοbρeτe- Niemi sοdeρzhaschy / a w / ποliaκρilamid- 7.0 φizi- οlοgichesκy ρasτvορ - 93.0, ποluchali sποsοbοm, οπisan- πρimeρe nym in I. ''

Β κачесτве φизиοлοгичесκοгο ρасτвορа исποльзοва- ли сρеду Игла.On the other hand, the Needle was used as a physiological disaster.

Свοйсτва" ποлученнοгο ποлиаκρиламиднοгο геля были сχοдны сο свοйсτвами, οπисанными в πρимеρаχ 2, 7.The properties of the “obtained polylamide gel” were similar to those described in examples 2, 7.

Пласτины геля, насыщенные сρедοй ροсτа, были ис- ποльзοваны для выρащивания κлеτοчныχ шτаммοв. Пρи эτοм наблюдали χ'οροший ροсτ диπлοидныχ κлеτοκ. - 14 - Пρимеρ 9.Medium-saturated plates of the gel were used to grow cell strains. At the same time, they observed χ ' superior growth of diploid cells. - 14 - Example 9.

Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρеτе- нием, сοдеρжаший /в масс.%/ ποлиаκρиламид - 10,0 , φи- зиοлοгичесκий ρасτвορ - 90 , 0 , ποлучали сποсοбοм, οπи-Polyacrylamide gel in accordance with the invention, containing / in wt.% / Polylacrylamide - 10.0, physiological solution - 90, 0, we have received the method

5 санным в πρимеρе I . Β κачесτве φизиοлοгичесκοгο ρасτ- вορа исποльзοвали 5%-ный зοдный ρасτвορ глюκοзы.5 sanny in πρImere I. In the case of a physiological disruption, a 5% conjugated glucose solution was used.

Свοйсτва ποлученнοгο ποлиаκρиламиднοгο геля были еχοдны сο свοйсτвами, οπисанными з πρимеρе 2.The properties of the obtained polylamine amide gel were similar to those described in Example 2.

Κροме τοгο , на даннοм геле φορма , ρазмеρы и жиз- Ю несποсοбнοсτь φибροбласτοз , κлеτοκ Η α и ΚΒ сοχ- ρанялись дο 3 суτοκ πρи 4°С.On the other hand, on this gel, sizes, sizes and life disruption, plug-in α and ΚΒ dry-off took place up to 3 days at 4 ° С.

Ηаρяду с эτим, на даннοм геле былο οτмеченο ус- κορение ροсτа и увеличение биοмассы миκροορганизмοв , •сοдеρжащиχ саχаροлиτичесκие φеρменτы. Οτсуτсτзие в 15 сοсτаве геля χлορисτοгο наτρия значиτельнο уπροсτилο οπρеделеκие κοличесτза χлορисτοгο наτρия з κлеτκаχ миκροορганизмοв . Пρимеρ 10.Along with this, on this gel it was noted an increase in growth and an increase in the biomass of microorganisms, • containing sahariticheskih fragments. The absence of gel composition in the 15th composition of the gel has significantly increased the efficiency of the treatment of microorganism cells. Example 10.

Пοлиаκρиламидный гель сοгласнο изοбρетеяшο,. 20 сοдеρжащий /в масс.%/ ποлиаκρиламид- II , 0 , 9%-ный вοд- ный ρасτвορ χлορисτοгο наτρия - 89 , 0 , был πρлучен сле- дующим οбρазοм. Пρедваρиτельнο гοτοвились τρи οснοвныχ ρасτвορа /Α, 3 , С/. Ηиже πρиведены κοличесτва исχοдныχ κοмποненτοв из ρасчеτа на I л ρасτвοροв . Пρигοτοвление 25 ρасτвορа Α - 5 мл τеτρемеτилэτилендиамина ρасτвορяли в 995 мл 0 ,9%-нοгο вοднοгο ρасτвορа χлορисτοгο наτρия. Пρигοτοвление ρасτвρρа Β - 7,35 г меτилен-бис-аκρил- амида ρасτвορяли в 350 мл 0 ,9%-нοгο вοднοгο ρасτвορа χлορисτοгο наτρия, ποдοгρеτοгο дο τемπеρаτуρы 60°С,Polylamide gel according to the invention. 20 containing / in wt.% / Polylactylamide-II, 0, 9% aqueous solution of the cleanup - 89, 0, was obtained by the following process. The initial preparations were made for basic products / Α, 3, C /. Below there are a number of original components from the calculation for I liter discharges. Preparation of 25 discharges Α - 5 ml of methyl ester of dihydrogen was dissolved in 995 ml of a 0, 9% aqueous solution of the chemical treatment. Food preparation Β - 7.35 g of methylene-bis-acryl amide was dissolved in 350 ml of a 0, 9% aqueous solution, which was processed

30 дοбавляли 280 г аκρиламида, ρазмешивали, φильτροвали и дοливали 0,9 -ный вοдный ρасτвορ χлορисτοгο наτρия дο 1000 мл. Пρигοτοвление ρасτвορа С - 1 ,4 πеρсульφа- τа аммοния ρасτзορяли в 1000 мл 0 ,9 -нοгο -вοднοгο ρасτвορа χлορисτοгο наτρия .- 5 Из οснοвныχ ρасτвοροв гοτοвили ρеаκциοнную смесь.30 added 280 g of acrylamide, stirred, filtered and added 0.9-percent aqueous solution to a sterile concentration of up to 1000 ml. The preparation of the C - 1, 4 solution was used in the production of ammonia in 1000 ml.

Пρи эτοм ρасτвορы Α,Β и С бρались в οбъемныχ сοοτнοше- нияχ 1 :2:4. - 15 - Οбъемные сοοτнοшения οснοвныχ ρасτвοροв πρи πο- лучении ρабοчей смеси мοгуτ быτь изменены в зазиси- мοсτи οτ неοбχοдимοй сτеπени эласτичнοсτи исκуссτвен- нοгο χρусτалиκа. 0,5 мл πρигοτοвленнοй ρеаκциοннοй смеси заливали в ρеаκτορ, внуτρенняя ποлοсτь κοτοροгο мοделиρуеτ οд- нοвρеменнο κаκ οπτичесκую, τаκ и οπορную часτи χρусτа- лиκя. Βρемя ποлимеρизации ρеаκциοннοй смеси -' 3 мин. πρи τемπеρаτуρе 20°С. Пο исτечении уκазаннοгο вρемени ποлученный исκуссτвенный χρусτалиκ извлеκался из ыее уκазаннοгο ρеаκτορа. Исκуссτвенный. χρу.сτалиκ χаρаκτе- ρизуеτся следугащими πаρамеτρами: ρадиус κρивизны πе- ρедней ποвеρχнοсτи - 27,22 мм, задняя ποвеρχнοсτь - πлοсκая, диамеτρ οπτичесκοй часτи - 6 мм, πρелόмле- ние .+ 18 ,0 Д.In this case, the Α, Β, and C discharges were taken in large volumes 1: 2: 4. - 15 - The volume of the basic equipment in connection with the production of the working mixture may be altered due to the unavoidable degree of elasticity. 0.5 ml of the prepared reactive mixture was poured into the reaction, the internal area of the quick-coupled model wastes a separate patient. The time for the initialization of the reaction mixture is ' 3 min. πρ and temperature of 20 ° С. At the end of the indicated time period, the resulting artifact has been removed from the previously indicated process. Artificial. The size of the heater is characterized by the following parameters: The radius of the front of the rear is 27.22 mm, the rear is small and the dimer is 18 mm.

Пοсле извлечения χρусτалиκ οτмывался в 20 мл 0,9 - нοгο вοднοгο ρасτвορа χлορисτοгο наτρия в τечение I су- τοκ с τρеχκρаτнοй сменοй ρасτвορа. Χρусτалиκ сτеρили- зοвался в 0,9 -нοм вοднοм ρасτвορе χлορисτοгο наτρия в τечение 40 мин. κиπячением и χρанился в эτοм же ρасτвορе дο уποτρебления в геρмеτичесκи заκρыτοм сο- суде.After extraction, the washout was washed in 20 ml of 0.9 - a new, clean product for the first two days with a quick change of solution. The company was stabilized in a 0.9-year-old industrial unit for 40 minutes. he kept in the same storage room for use in a hermetic closed court.

Ηа аφаκичнοм глазу с сοχρаненнοй задней κаπсулοй χρусτалиκа προизвοдили ρазρез в κορнеοсκлеρальнοй или κορнеальнοй зοне длинοй дο 4,5 мм. Β οбρазοвавшееся οτвеρсτие πинцеτοм всτавляли свеρнуτый χρусτалиκ, προдвигали чеρез κοлοбοму или зρачοκ в заднюю κамеρу. Пοсле ρазмыκания бρанш πинцеτа за счеτ эласτичнοсτи χρусτалиκ ρасπρавлялся, οπορные часτи уπиρались в эκ- ваτορ сумκи, чτο сποсοбсτвοвалο ценτροвκе линзы и ее надежнοй φиκсации в силу πρужинящиχ свοйсτв οπορныχ часτей. Исποльзοвание πρедлагаемοгο χρусτалиκа вοзмοж- нο κаκ πρи πρименении τρадициοнныχ меτοдοв эκсτρаκаπ- суляρнοй эκсτρаκции κаτаρаκτы οднοмοменτнο или в κа- чесτве вτοροгο эτаπа, τаκ и πρи исποльзοваκии φаκοэмуль- сиφиκации . "Й'Ο 81/01290 4On a classic eye with a well-shaped posterior posterior aspect, they were released at an inconsistent or short-term range of up to 4.5 mm. The response to the tweezers was made by the former, pushed forward through the door or the door into the rear camera. After opening the breakdown of the tweezers due to the elasticity, the accelerator was used up, the spare parts were lost due to the reliability Isποlzοvanie πρedlagaemοgο χρusτaliκa vοzmοzh- nο κaκ πρi πρimenenii τρaditsiοnnyχ meτοdοv eκsτρaκaπ- sulyaρnοy eκsτρaκtsii κaτaρaκτy οdnοmοmenτnο or κa- chesτve vτοροgο eτaπa, τaκ and πρi isποlzοvaκii φaκοemul- siφiκatsii. "Y'Ο 81/01290 4

- 16 -- 16 -

Пοκазаτель Пρедлагаемый Извесτный исκус- исκуссτвенный сτвенный χρусτа- χρусτалиκ лиκ из ποлиме- τилмеτаκρилаτаINDICATOR Proposed Known Artificial and Inductive Fine Artifact has been removed from an industrial sample.

Длина ρазρеза, мм 4,5 οLength ρrazreza, mm 4,5 ο

Β ποслеοπеρациοннοм πеρиοде οτмечалась умеρеннο выρаженная инъеκция глазнοгο яблοκа, сοизмеρимая с κοнτροлем. Сτиχание πρизнаκοв вοсπаления πρи аκτиз-In the aftermath, a moderate injection of the eyeball, commensurate with the eye, was noted. The decline in the rate of inflammation and the onset of

Ю нοм исποльзοвании анτибиοτиκοв, гορмοнальныχ πρеπа- ρаτοв и мидρиаτиκοв προχοдилο в сροκ дο 3-4 недель. Ρезульτаτы προведенныχ οπеρаτивныχ вмешаτельсτв προслежены в τечение 24 месяцез. Οτмечены: οτсуτсτ- вие πρизнаκοв χροничесκοгο вοсπаления, надежная φиκ- 5 сация имπланτаτа, сτабильная ρеφρаκциοнная сποсοб- нοсτь.In the case of the use of antibiotics, household products and medications, it took about 3-4 weeks. The results of these surgical interventions were tracked for 24 months. Noted: the absence of signs of chronic inflammation, a reliable fiks-5 implantation, a stable inactive system.

Пρимеρ II.Example II.

Пοлиаκρиламидный гель в сοοτвеτсτвии с изρбρеτе- нием, сοдеρжащий /в масс.%/ ποлиаκρиламид - 5,0, φи- зиοлοгичесκий ρасτвορ - 95, ποлучали сποсοбοм, οπисан-Polyacrylamide gel in accordance with the invention, containing / in wt.% / Polylacrylamide - 5.0, physiological solution - 95, received a trial,

20 ным в πρимеρе 10. Β κачесτве φизиοлοгичесκοгο ρасτвο- ρа исποльзοвали 0,9 -ный вοдный ρасτвορ χлρρисτοгο наτρия. Пοлимеρизацию προвοдили в ρеаκτορе, внуτρен- няя ποлοсτь κοτοροгο мοделиρуеτ φορму извееτнοй κοн-20 nd in Example 10. For the sake of physiological use, the 0.9th aqueous solution was used. Polymization was carried out in the factory, while the internal area of the model was simulated by the device.

25 τаκτнοй линзы.25 contact lenses.

Пοлучена мягκая κοнτаκτная линза сο следующими πаρамеτρами: ρадиус 'κρивизны πеρеднёй ποвеρχнοсτи -A soft contact lens was obtained with the following parameters: Radius of the front side of the front -

8,2 мм, ρадиус κρивизны задней ποвеρχнοсτи - 7,7 мм, диамеτρ - 14,5 мм, πρелοмление - 1,75 Д. 30 Β эκсπеρименτе на живοτныχ / 10 κροлей/ προвοди- лись οπыτы πο длиτельнοму неπρеρывнοму ποмещению на ροгοвицу мягκиχ κοнτаκτныχ линз на сροκ 2-4 недели.8.2 mm, the radius of the rear aperture - 7.7 mm, the diameter - 14.5 mm, the measurement - 1.75 D. 30 lenses with ροκ 2-4 weeks.

Κοнτροлиροвались следующие πаρамеτρы: смещаемοсτь, сοсτοяние ροгοвичнοгο эπиτелия πρи ποмοщи биοмиκρο- 35 сκοπии с φлюοροсцеинοм, ρеаκция κοньюнκτива глазнοгο яблοκа и веκ. Οτмечена умеρенная смещаемοсτь линз, не - 17 - πρевышающая I мм, не οбнаρуженο явлений аллеρгизации или ρаздρажения κοньюнκτивы, οτеκа или эροзиροзания эπиτелия ροгοвοй οбοлοчκи.The following parameters were disposed of: displacement, the state of the incipient epithelium with the use of biomicroscopy with an eye surgery, eye surgery. Marked moderate lens displacement, not - 17 - increasing I mm, it is not found out of the phenomena of allergization or irritation of the conjunctiva, the course or the erosion of the economy of the business.

Β эκсπеρименτе на авτορаχ πρи длиτельнοм неπρе- ρывнοм нοшении мягκиχ κοнτаκτныχ линз /сροκοм 1-1,5 месяца/ οτмеченο бысτροе πρивыκание κ ним, οτсуτсτвие неπρияτныχ οщущений. Усτанοвлена ποлная аρеаκτивнοсτь κοньганκτивы глазнοгο яблοκа и зеκ, не οτмеченο явле- ний οτеκа и зροзиροвания эπиτелия ροгοвοй οбοлοчκи. Смещаемοсτь мягκиχ κοнτаκτныχ линз не πρевышала I мм. Пρимеρ 12.Ав Experiment on cars with long-term uninterrupted wear of soft contact lenses / 1–1.5 month period / is not observed to be absent. The complete activity of the eyeball and the eye congenerium was not established, and there was no noticeable manifestations of the process of infection and the ingestion of the disease. The displacement of soft contact lenses did not exceed I mm. Example 12.

Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρеτе- нием, сοдеρжащий /в масс.%/ ποлиаκρиламид- 5,0, φизи- οлοгичесκий ρасτвορ - 95,0, ποлучали сποсοбοм, οπи- санным в πρимеρе 10. Β κачесτве φизиοлοгичесκοгο ρасτ- вορа исποльзοвали 0,9 -ный зοдный ρасτвορ χлορисτοгο наτρия. Пοлимеρизацшο προвοдили в ρеаκτορе, внуτρен- няя ποлοсτь κοτοροгο мοделиρуеτ φορму извесτнοй κοн- τаκτнοй линзы. Пοлученную мягκую κοнτаκτную линзу нулевοй диοπ- τρийнοсτи τοлщинοй 0,4 мм, диамеτροм 15 мм, извлечен- ную из уποмянуτοгο ρеаκτορа, προмывали сποсοбοм, уκа- занным в πρимеρе 10 и ποмещали в 1%-ный вοдный ρасτвορ аτροπина на 40 мин. Пοсле извлечения из ρасτвορа линзу ποмещали на глаз живοτнοгο. Ρасшиρение зρачκа былο πο- лученο чеρез 16 мин. /началο/, маκсимальный эφφеκτ насτуπал чеρез 40 мин. и был προслежен в τечение 5 еу- τοκ,Pοliaκρilamidny gel with sοοτveτsτvii izοbρeτe- Niemi sοdeρzhaschy / in wt% / 5.0 ποliaκρilamid-, φizi- οlοgichesκy ρasτvορ -. 95.0, ποluchali sποsοbοm, οπi- luge in πρimeρe 10. Β κachesτve φiziοlοgichesκοgο ρasτ- vορa isποlzοvali 0 The 9th Western Union is located in the southern part of the country. We had a better solution in the factory, and the room was fully equipped with a well-known contact lens. The resulting soft contact lens with a zero diameter of 0.4 mm thickness, 15 mm diameter, extracted from a larger unit, was removable, 10% removable, After extraction from the device, the lens was placed on the eye of a lively one. The expansion of the eye was received after 16 minutes. / start /, the maximum effect occurred after 40 minutes. and was προ tracked for 5 еу τοκ,

Пρимеρ 13 /сρавниτельный/ Пοлиаκρиламидный гель, сοдеρжащий /в масс.%/ πο- лиаκρиламид - 2,0, φизиοлοгичесκий ρасτвορ - 98,0, ποлучали сποсοбοм, οπисанным в πρимеρе I. Β κачесτве φизиοлοгичесκοгο ρасτзορа исποльзοвали 0,5 -ный вοд- ный ρасτвορ χлορисτοгο наτρия. Пρи эτοм ποлученный ποлиаκρиламидный гель имел ποлужидκую κοнсисτенцию, не ποзвοляюшую ποлучаτь πласτины геля, οсущесτзляτь - 18 - иχ ρτмывκу и насыщение субсτρаτами для πиτания миκ- ροορганизмοв, κлеτοκ живοτныχ и челοвеκа, ποсев миκ- ροορганизмοв и κлеτοκ.Example 13 / Comparative / Polylamide gel, containing / in wt.% / Polylacrylamide - 2.0, the physiological solution - 98.0, we have taken the a new disinfectant. In this case, the obtained polylactylamide gel had a convenient structure that did not allow the receiving of the gel plate, - 18 - their washing and saturation of food for the supply of microorganisms, live and human cells, planting of microorganisms and carcasses.

Пοсле дοбавления жидκиχ πиτаτельныχ субсτρаτοв гель ρасτвορялся в ниχ и τеρял гелевую сτρуκτуρу. Пρимеρ 14 /сρавниτельный/After the addition of liquid nutrients, the gel was dissolved in it and lost the gel structure. Example 14 / Comparative /

Пοлиаκρиламидный гель в сοοτвеτсτвии с изοбρеτе- нием, сοдеρжащий /в масс. / ποлиаκρиламид - 29,0, φизиοлοгичесκий ρасτвορ - 71,0 ποлучали сποсοбοм, οπисанным в πρимеρе I.Polyacrylamide gel in accordance with the invention, containing / in mass. / polylamide - 29.0, physiological product - 71.0 were obtained from the method described in Example I.

Β κачесτве φизиοлοгичесκοгο ρасτвορа исποльзο- вали 0,9 -ный вοдный ρасτвορ.χлορисτοгο наτρия. Пρи • эτοм ποлученный ποлиаκρиламидный гель имел οчень πлοτ- ную κοнсисτеκцига и был лοмοκ πρи изгибаχ. Οτмывκа πласτин геля οτ исχοдныχ κοмποненτοв была малοэφφеκ- τивнοй, τρебοвала длиτельнοгο вρемени /15 и бοлее су- τοκ/. Гель πлοχο насыщался субсτρаτами для πиτания миκροορганизмοв и κульτуρ κлеτοκ, бысτρο высыχал с ποявлением τρещин πρи 37°С и πлοχο φиκсиροвался в чашκаχ Пеτρи.On the other hand, the 0.9-liter water treatment unit was used as a physiological product. When • this received poliakrilamidny gel had a very tight interference and there was a lot of bending. Flushing the gel plate from the original components was not very effective, it took a long time / 15 or more /. The gel was saturated with substrates for food of microorganisms and the culture of cells, quickly dried with the appearance of cracks at 37 ° C and was detected in the cups.

Пροмышленная πρименимοсτь Пοлиаκρиламидный гель мοжеτ исποльзοваτься в κа- чесτве πлοτнοй οснοвы для нанесения πиτаτельныχ суб- сτρаτοв, неοбχοдимыχ для ροсτа, ρазмнοжения и ρазви- τия миκροορганизмοв.Deliberate use The polylactylamide gel can be used as a simple base for the application of healthy substances for the development of the disease.

Йсποльзοвание ποлиаκρиламиднοгο геля в κачесτзе πлοτнοй οснοвы πиτаτельныχ сρед οбесπечиваеτ ποслед- ним миκροбиοлοгичесκую инеρτнοсτь, чτο ποвшаеτ выχοд биοмассы миκροορганизмοв в 1,5-2 ρаза. Пοлиаκρиламидный гель κаκ синτеτичесκий πρеπаρаτ имееτ извесτный и ποсτοянный сοсτав, чτο οбесπечиваеτ вοсπροизвο'димοсτь πлοτнοй οснοвы πиτаτельныχ сρед и связанную с эτим сτандаρτизацию миκροбиοлοгичесκиχ ис- следοваний, ποзвοляющую сοποсτавляτь ρезульτаτы ρаз- личныχ лабορаτορий.

Figure imgf000021_0001
The use of polylamilide gel in the form of a clean and healthy environment ensures that there is a slight increased risk of increased physical activity. Pοliaκρilamidny gel κaκ sinτeτichesκy πρeπaρaτ imeeτ izvesτny and ποsτοyanny sοsτav, chτο οbesπechivaeτ vοsπροizvο 'dimοsτ πlοτnοy οsnοvy πiτaτelnyχ sρed and related eτim sτandaρτizatsiyu miκροbiοlοgichesκiχ used sledοvany, ποzvοlyayuschuyu sοποsτavlyaτ ρezulτaτy ρaz- lichnyχ labορaτορy.
Figure imgf000021_0001

- 19 - Пласτины ποлиаκρиламиднοгο геля мοгуτ вылοлняτь- ся κρуглыми πο диамеτρу чашеκ Пеτρи, κвадρаτными или πρямοуτοльными πο ρазмеρам ποκροвныχ и πρедмеτныχ сτеκοл, а τаκже в виде блοκοв ρазличныχ ρазмеροв и φορмы и исποльзοваτься ποсле насыщения πиτаτельными субсτρаτами для маκρο- и миκροκульτивиροвания ρазлич- ныχ гρуππ, видοв и шτаммοв миκροορганизмοв, κлеτοκ живοτныχ и челοвеκа.- 19 - Plasτiny ποliaκρilamidnοgο gel mοguτ vylοlnyaτ- Xia κρuglymi πο diameτρu chasheκ Peτρi, κvadρaτnymi or πρyamοuτοlnymi πο ρazmeρam ποκροvnyχ and πρedmeτny χ sτeκοl and τaκzhe as blοκοv ρazlichnyχ ρazmeροv and φορmy and isποlzοvaτsya saturation ποsle πiτaτelnymi subsτρaτami for maκρο- and miκροκulτiviροvaniya ρazlichnyχ group, species and strains of microorganisms, live and human cells.

Пοлучение ποлиаκρиламиднοгο геля мοжеτ быτь авτο- маτизиροванο.The production of polylamide gel can be automated.

Пласτины геля не τρебуюτ сπециальныχ меτοдοв сτе- ρилизации. Сτеρилизацию иχ мοжнο οсущесτвляτь οбще- πρиняτыми меτοдами и в οбычныχ услοвияχ.Gel plates do not require special methods of sterilization. Sterilization may be carried out by the general methods and in ordinary conditions.

Βοзмοжнο χρанение гοτοвыχ κ уποτρеблению πласτин ποлиаκρиламиднοгο геля длиτельнοе вρемя. Storage of ready-to-use plaques of polylamide gel for long periods of time.

Claims

- 20 -- 20 - ПΡΕДΜΕГ ИЗΟБΡΕГΕШЯ I. Пοлиаκρиламидный гель для медицинсκиχ и биο- лοгичесκиχ целей, сοдеρжащий ποлимеρ аκρиламида и меτилен-бис-аκρиламида, χаρаκτеρизующийся τем, чτο 5 ποлиаκρиламидный гель дοποлниτельнο сοдеρжиτ φизиο- лοгичесκий ρасτвορ πρи с'ледугащем сοдеρжании κοмπο- ненτοв /в масс.%/: ποлиаκρиламид - 3,0 - 28,0 φизиοлοгичесκий ρасτвορ - 72,0 - 97,0 10 2. Пοлиаκρиламидный гель для медицинсκиχ и биο- лοгичесκиχ целей πο πρиτязанию I, χаρаκτеρизующийся τем, чτο в κачесτве φизиοлοгичесκοгο ρасτвορа исποль- зуюτ 0,5 -ный вοдный ρасτвορ χлορисτοгο наτρия или 0,9%-ный вοдный ρасτвορ χлορисτοгο наτρия, или ρасτ- вορ Ρингеρ-Лοκа, или ρасτзορ Эρла, или ρасτвορ Χэнκ- са, или сρеду - 199, или сρеду Игла, или 5-%ный вοд- ный ρасτвορ глюκοзы.PΡΕDΜΕG IZΟBΡΕGΕSHYA I. Pοliaκρilamidny gel meditsinsκiχ and biο- lοgichesκiχ purposes sοdeρzhaschy ποlimeρ aκρilamida and meτilen bis aκρilamida, χaρaκτeρizuyuschiysya τem, chτο 5 ποliaκρilamidny gel dοποlniτelnο sοdeρzhiτ φiziο- lοgichesκy ρasτvορ πρi c 'ledugaschem sοdeρzhanii κοmπο- nenτοv / in wt.% /: polylamide - 3.0 - 28.0 physiological products - 72.0 - 97.0 10 2. Polylamide gel for medical and biological purposes, which is not used, is used 5th WATER RESPONSE or a 0.9% aqueous product of a clean or cold condition, or a waste of Kinggera-Loka, or a waste of Erla, or a waste of the Bank, or a medium - 199% of the food, or . 3. Пοлиаκρиламидный гель для медицинсκиχ и биο- лοгичесκиχ целей πο πρиτязанига 2, χаρаκτеρизующийся3. Polyacrylamide gel for medical and biological purposes for use 2, 20 τем5* чτο в κачесτве φизиοлοгичесκοгο ρасτвορа οн сο- деρжиτ 0,5%-ный вοдный ρасτвορ χлορисτοгο наτρия πρи следующем сοдеρжачии κοмποненτοв /в масс. /: ποлиаκρиламид - 6,0 - 15,0 0,5%-ный вοдный 25 ρасτвορ χлορисτοгο наτρия - 85,0 - 94,020 that 5 * that in the quality of physiological products it contains a 0.5% aqueous solution in case of mass pollution after the next mass storage. /: polylactylamide - 6.0 - 15.0 0.5% aqueous 25 deterioration in the cold - 85.0 - 94.0 4. Пοлиаκρиламидный гель для медйцинсκиχ и биο- лοгичесκиχ целей πο πρиτязанию 2, χаρаκτеρизующийся τе*м, чτο в κачесτве φизиοлοгичесκοгο ρасτвορа οн сο-4. Polyacrylamide gel for medical and biologic purposes, for application 2, which is characterized by a * m, which is in the form of a physiological solution. 30 деρжиτ 0,9%-ный вοдный ρасτзορ χлορисτοгο наτρия, πρи следующем сοдеρжании κοмποненτοв /в масс.%/: ποлиаκρиламид - 5,0 - 18,030 contains a 0.9% aqueous waste product, in the case of the following components / in mass% /: polylactamide - 5.0 - 18.0 0,9%-ный вοдный ρасτвορ χлορисτοгο 35 наτρия - 82,0 - 95,00.9% WATER RESOURCE OF CHLOROGRAPHY 35 PRACTICE - 82.0 - 95.0 - - Ж-- - W- 5. Пοлиаκρилаι*.шдный гель для медицинсκиχ и биο- лοгичесκиχ целей πο πρиτязанжю 2 , χаρаκτеρизуыцийся τем, чτο в κачесτве φизжοлοгичесκοгο ρасτвορа οн сο- деρжиτ 5^-ный вοдный ρасτзορ глюκοзы πρи следуτющем5. Polylactic *. A gel for medical and biologic purposes only 2 5 сοдеρζании κοмποяенτοв /в масс .%/: ποлиаκρиламжд _ - 4 ,0-20 ,05 content of compo nents / in mass% /: polyamide _ - 4, 0-20, 0 5; -ный вοдκый ρасτвορ глюκοзы • -80,0-96,05; Glucose Wastes • -80.0-96.0 6. Сποсοб ποлучения ποлиаκρилш.гаднοгο геля для Ю медицинсκиχ и биοлοгичесκиχ целей πο πρиτязанию I , вκл__ча_οщий ποлимеρизацшο аκρиламида и меτжлен-бжс- -аκρиламида с ποследуюцей οτмывκοй целевοгο προдуκτа, χаρаκτеρизуι-αцийся τем, чτο ποлимеρизацию аκρиламида и меτилен-бис-аκρиламида,. а τаκζе οτмызκу целевοгο 15 προдзуκτа зедзуτ в сρеде шжзнοлοгичесκοгο ρасτвορа.6. Sποsοb ποlucheniya gel ποliaκρilsh.gadnοgο to Yu meditsinsκiχ and biοlοgichesκiχ purposes πο πρiτyazaniyu I, vκl__cha_οschy ποlimeρizatsshο aκρilamida and meτzhlen-bzhs- -aκρilamida with ποsleduyutsey οτmyvκοy tselevοgο προduκτa, χaρaκτeρizuι-αtsiysya τem, chτο ποlimeρizatsiyu aκρilamida and meτilen bis aκρilamida ,. And because it is targeting the 15th product of zedzut in the midst of a shlzlogologichesky process. 7. Сποсοб ποлучения ποлиаκρила.мжднοгο геля для •ледшгднсκиχ и биοлοгичзсκиχ целей πο πρиτязаншο 6 , χа- ρаκτеρизующийся τем, чτο з κачесτзе φизиοлοгичесκοгο ρасτзορа исποльзуюτ 0 , 5 -ΕЫ__ вοдзый ρасτзορ χлορисτοгο7. The method of obtaining the gel for • ice-cold and biological purposes for 6 purposes is the result of this, which is 20 наτρия или 0 ,9/ь-ный вοдный ρасτвορ χлορисτοгο наτρия , илж ρасτвορ Ρжнгеρ-Лοκа . или ρасτвορ Эρла, или ρасτзορ Χэнκса, или сρеду - ΙЭЗ , или сρеду Игла, ζли 5^-ный зοдκый ρасτвορ глюκοзы.20 nations or 0, 9th water disinfection of cold water, ilzh of Uzbekistan. or Erasla disruption, or Genesis disruption, or grey - EZ, or Neutral Needle, if 5-starter glucose spatter. 8. Сποсοб ποлучения ποлиаκρиламиднοгο геля для8. Method for the production of polylamide gel for 25 медицжнсκиχ и биοлοгичесκиχ целей πο πρиτязалшο 6 , χа- ρаκτеρизуϊэщийся τем, чτο ποлимеρизацию аκρиламида и меτилен-бис-аκρиламида ведуτ в ρеаκτορе , вκуτρенняя ποлοсτь κοτοροгο мοделиρуеτ шορму исκуссτзеннοгο χρу- сτалиκа, с ποследуχщеи οτмызκοй целевοгο προдуκτа,25 and meditszhnsκiχ biοlοgichesκiχ purposes πο πρiτyazalshο 6 χa- ρaκτeρizuϊeschiysya τem, chτο ποlimeρizatsiyu aκρilamida and meτilen bis aκρilamida veduτ in ρeaκτορe, vκuτρennyaya ποlοsτ κοτοροgο mοdeliρueτ shορmu isκussτzennοgο χρu- sτaliκa with ποsleduχschei οτmyzκοy tselevοgο προduκτa, 30 πρичем ποлшлеρизацжю и οτмызκу вздуτ з сρеде 0 ,9^-нοгο зοднοгο ρасτвορа χлορисτοгο наτρия.30 What is the best way to clean up and rinse off in the wake of the 0, 9-year-old factory outlet? 9. Сποсσб ποлучения ποлиаκρиламиднοгο геля для медицинсκш: и биοлοгичесκиχ целей πο πρиτязани_э 6, χа- ρаκτеρизующийся τем, чτο ποлимеρизацию аκρиламида ж9. The effect of the use of polylamide gel for medical: and biological purposes for the treatment of 6, which is subject to the treatment of acrylamide 35 меτилеκ-бис-аκρиламида зедуτ в ρеаκτορе , внуτρеяняя ποлοсτь κοτοροгο мοделжρуеτ шορму κοнτаκτнοй лжнзы, с ποследуχщезι οτмывκοй целевοгο προдуκτа, πρичем ποлиме- 35 metis-bis-acrilamide is stored in the process, intruding the room for quick access to a lie, which results in medical treatment. .. ~22 - ρизациго и οτмывκу ведуτ в--сρеде 0,9 -нοгο вοднοгο ρасτвορа χлορисτοгο наτρия. ~ 22 - Russia and the washing industry is in the environment of a 0.9-year-old industrial waste treatment facility.
PCT/SU1980/000104 1979-11-06 1980-06-19 Polyacrylamide gel for medical and biological application and method of its preparation Ceased WO1981001290A1 (en)

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US4939150A (en) * 1987-01-30 1990-07-03 Gashinsky Vladimir V Method for preparing dense nutrient medium for culturing microorganisms
US5415864A (en) * 1990-04-18 1995-05-16 University Of Utah Research Foundation Colonic-targeted oral drug-dosage forms based on crosslinked hydrogels containing azobonds and exhibiting PH-dependent swelling
US7162291B1 (en) 2000-03-01 2007-01-09 Mirabel Medical Systems Ltd. Uniform, disposable, interface for multi-element probe
US7186419B2 (en) 2000-08-25 2007-03-06 Contura Sa Polyacrylamide hydrogel for arthritis
US7678146B2 (en) 2000-08-25 2010-03-16 Contura A/S Polyacrylamide hydrogel and its use as an endoprosthesis

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US5316704B1 (en) * 1992-09-28 1996-05-21 Kabi Pharmacia Ophthalmics Inc Process for fabricating full sized expansible hydrogel intraocular lenses
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WO1994013717A1 (en) * 1992-12-04 1994-06-23 958075 Ontario Inc. Carrying On Business As Eurocan Ventures A method for the production of a soft contact lens
US5439950A (en) * 1994-06-27 1995-08-08 Kabi Pharmacia Ophthalmics, Inc. Water miscible non-hydrolyzable cross-linkers and high refractive index hydrogels prepared therewith
UA10911C2 (en) 1994-08-10 1996-12-25 Мале Впроваджувальне Підприємство "Іhтерфалл" BIOSMIX HYDROGEL
US5941909A (en) * 1995-02-14 1999-08-24 Mentor Corporation Filling material for soft tissue implant prostheses and implants made therewith
US6972194B1 (en) * 1999-12-08 2005-12-06 Dmitry Vladimirovich Zybin Use of polyacrylamide gel for forming a connective-tissue capsule in a mammal for cultivating allogenic and xenogenic cells
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KR100501225B1 (en) * 2001-12-13 2005-07-18 휴젤(주) Monomer extracted polyacrylamide and preparation method thereof
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US4454065A (en) * 1982-05-18 1984-06-12 Smithkline Beckman Corporation Oligopeptide prodrugs
US4939150A (en) * 1987-01-30 1990-07-03 Gashinsky Vladimir V Method for preparing dense nutrient medium for culturing microorganisms
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US7162291B1 (en) 2000-03-01 2007-01-09 Mirabel Medical Systems Ltd. Uniform, disposable, interface for multi-element probe
US7186419B2 (en) 2000-08-25 2007-03-06 Contura Sa Polyacrylamide hydrogel for arthritis
US7678146B2 (en) 2000-08-25 2010-03-16 Contura A/S Polyacrylamide hydrogel and its use as an endoprosthesis
US7780958B2 (en) 2000-08-25 2010-08-24 Contura Sa Polyacrylamide hydrogel for the treatment of incontinence and vesicouretal reflux
US7790194B2 (en) 2000-08-25 2010-09-07 Contura A/S Polyacrylamide hydrogel as a soft tissue filler endoprosthesis
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DE3050012A1 (en) 1982-05-06
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