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UA95602C2 - Иммуногенная композиция цвс2 и способы приготовления такой композиции - Google Patents

Иммуногенная композиция цвс2 и способы приготовления такой композиции

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Publication number
UA95602C2
UA95602C2 UAA200708620A UAA200708620A UA95602C2 UA 95602 C2 UA95602 C2 UA 95602C2 UA A200708620 A UAA200708620 A UA A200708620A UA A200708620 A UAA200708620 A UA A200708620A UA 95602 C2 UA95602 C2 UA 95602C2
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expressed
protein
compositions
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Ukrainian (uk)
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Марк ЭЙЧМЕЙЕР
Грег Нитзель
Меррилл Шаффер
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Берингер Ингельхейм Ветмедика, Инк.
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Application filed by Берингер Ингельхейм Ветмедика, Инк. filed Critical Берингер Ингельхейм Ветмедика, Инк.
Publication of UA95602C2 publication Critical patent/UA95602C2/ru

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Abstract

Изобретение относится к способу восстановления протеина, экспрессированного открытой рамкой считывания 2, из цирковируса свиньи 2. Способ включает этапы трансфектирования рекомбинантного бакуловируса, который содержит открытую рамку считывания 2, который кодирует последовательности в клетках, помещенных в ростовую среду, которая заставляет вирус экспрессировать открытую рамку считывания 2, и восстановление экспресированного протеина в супернатанте. Это восстановление происходит приблизительно через 5 дней после инфицирования клеток, чтобы достаточное количество рекомбинированного протеина экспрессировалось и секретировалось клеткой в питательную среду. Такие способы предохраняют от дорогих и трудоемких методик экстракции, которые нужны для выделения и восстановления рекомбинантного протеина в клетках.
UAA200708620A 2004-12-30 2005-12-29 Иммуногенная композиция цвс2 и способы приготовления такой композиции UA95602C2 (ru)

Applications Claiming Priority (1)

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US64051004P 2004-12-30 2004-12-30

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UA95602C2 true UA95602C2 (ru) 2011-08-25

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UAA200708620A UA95602C2 (ru) 2004-12-30 2005-12-29 Иммуногенная композиция цвс2 и способы приготовления такой композиции
UAA201015883A UA107177C2 (uk) 2004-12-30 2010-12-29 Імуногенна композиція цвс2 та спосіб приготування такої композиції

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UAA201015883A UA107177C2 (uk) 2004-12-30 2010-12-29 Імуногенна композиція цвс2 та спосіб приготування такої композиції

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US (2) US9919041B2 (ru)
UA (2) UA95602C2 (ru)

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US9919041B2 (en) 2018-03-20

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