TWM602905U - Probiotic particles with core-shell structure - Google Patents

Abstract

A probiotic particle with a core-shell structure, comprising a core, an intermediate layer formed on the outer surface of the core, and a coating layer formed on the outer surface of the intermediate layer, wherein the core contains at least one probiotic Bacteria, the intermediate layer contains a first substance, the first substance has a first isoelectric point ranging from pH 1 to pH 6, and the coating layer contains a second substance, the second substance has a A second isoelectric point in the range of pH 4 to pH 8 and higher than the first isoelectric point, wherein the second isoelectric point and the first isoelectric point have a pH between 0.5 and 3 Difference.

Description

Probiotic particles with core-shell structure

This new model relates to a probiotic particle with a core-shell structure, in particular to a probiotic particle that can protect the probiotic components from being destroyed by gastric acid and has good dispersibility in neutral and alkaline solutions.

Probiotics (Probiotics) [comprising Lactobacillus (Lactobacillus), the genus Bifidus (Bifidobacterium), Bacillus (Bacillus), Lactococcus (Lactococcus), Enterococcus genus (Enterococcus), Saccharomyces (Saccharomyces) and Streptococcus , etc.] can selectively stimulate the growth of native bacteria in the intestine, change the composition, quantity or nature of the intestinal microflora, and promote the health of the host’s intestinal tract. The host's immune response has been widely used in food and health products as a microbial food ingredient. However, most of the commercially available health foods containing probiotics are not resistant to strong acid and are easily destroyed by stomach acid after being taken by the human body. Therefore, how to protect the probiotic components in food or health products so that they can reach the intestinal tract smoothly to improve the intestinal health of the human body has become the goal of relevant researchers in this field.

After research, the applicant unexpectedly discovered that after the first substance and the second substance are used to coat the probiotics in sequence, the formed probiotic particles with a core-shell structure can condense into clusters in an environment with a pH value of less than 4 Block to reduce the destruction of probiotics by stomach acid. In addition, the probiotic particles have a high degree of dispersibility in an environment where the pH value is greater than 6, unlike the traditional dry probiotic powder, which is difficult to uniformly disperse in the solution.

Therefore, the purpose of the present invention is to provide probiotic particles that protect probiotics from being destroyed by gastric acid, have good dispersibility in neutral and alkaline solutions and can resist aggregation.

Therefore, the probiotic particles with a core-shell structure of the present invention comprise a core, a middle layer formed on the outer surface of the core, and a coating layer formed on the outer surface of the middle layer (coating layer), wherein the core contains at least one probiotics, the intermediate layer contains a first substance, the first substance has a first isoelectric point ranging from pH 1 to pH 6, and The coating layer contains a second substance that has a second isoelectric point in the range of pH 4 to pH 8 and higher than the first isoelectric point, wherein the second isoelectric point and the The first isoelectric point has a pH difference between 0.5 and 3. The probiotic particles with a core-shell structure can be processed into a dosage form of a probiotic soft capsule. The probiotic soft capsule contains a capsule shell and a plurality of probiotic particles with a core-shell structure. The capsule shell forms a closed capsule. In the containing space, the probiotic particles with core-shell structure are contained in the containing space.

The effect of the new model is that the probiotics are sequentially coated by the first substance and the second substance, and the formed core-shell structure can effectively protect the probiotics, so that the probiotics can be aggregated in an environment with a pH value of less than 4 Clumps to reduce the destruction of probiotics by stomach acid. In addition, the probiotic particles have a high degree of dispersibility in an environment where the pH value is greater than 6, and can improve the problem that the traditional dry probiotic powder is not easy to be uniformly dispersed in the solution.

1, a preferred embodiment of the new type of probiotic particles 1 with a core-shell structure comprises a core 11, an intermediate layer 12 formed on the outer surface of the core 11, and an outer layer formed on the intermediate layer 12 Surface coating layer 13.

The core 11 contains at least one probiotics. Preferably, the probiotic is selected from the group consisting of Bacillus spp., Streptococcus spp., Lactococcus spp., Nutrition Defective bacteria species ( Abiotrophia spp.), Aerococcus spp., Aerococcus spp., Carnobacterium spp., Enterococcus spp., Lactobacillus spp., Leuconostoc spp., Oenococcus spp., Pediococcus spp., Tetragenococcus spp., Vagococcus spp.), Weissella species (Weissella spp.), species of genus Bifidus (Bifidobacterium spp.), yeast species (Saccharomyces spp.), Kluyveromyces species (Kluyveromyces spp.), Staphylococcus spp., Pediococcus spp., Propionibacterium spp., and combinations thereof. In a preferred embodiment of the present invention, the probiotic is a combination of Lactobacillus plantarum and Bifidobacterium longum .

In a preferred embodiment of the present invention, the core 11 may further include a pharmaceutically acceptable carrier that is widely used in pharmaceutical manufacturing technology. For example, the pharmaceutically acceptable carrier may include one or more reagents selected from the group consisting of solvents, buffers, emulsifiers, suspending agents, decomposers ), disintegrating agent, dispersing agent, binding agent, excipient (for example, starch, dextrin, lactose, corn Maize flour, rice flour, tricalcium phosphate, silicon dioxide, magnesium stearate, calcium carbonate), stabilizing agent), chelating agent, diluent, gelling agent, preservative, filler, wetting agent, lubricant, Absorption delaying agent, liposome, sweetening agent, flavoring agent (for example, glucose, sucrose, fructose, sugar alcohol) , Oligosaccharide, sugar substitute, fruit powder, yeast powder, corn flour, nonfat dry milk, casein, whey protein ( whey protein, amino acid, citric acid, citrate, lactic acid, lactate, nucleotide (nucleotide)), coloring agent) and the like. The selection and quantity of these reagents fall within the scope of professionalism and routine techniques of those who are familiar with this technique.

The intermediate layer 12 contains a first substance, and the first substance has a first isoelectric point in the range of pH 1 to pH 6. Preferably, the first substance is selected from the group consisting of nonfat dry milk (nonfat dry milk), casein (casein), whey protein (whey protein), soybean protein (soybean protein), Pea protein, egg protein, rice protein, hydrolyzed protein, zein, wheat protein, barley protein, branched chain Branched chain amino acid, gelatin, collagen, amino acid, chitosan, chitin, and combinations thereof. In a preferred embodiment of the present invention, the first substance is whey protein.

The coating layer 13 contains a second substance, and the second substance has a second isoelectric point in the range of pH 4 to pH 8 and higher than the first isoelectric point. Preferably, the second substance is selected from the group consisting of: sodium alginate, agar, carrageenan, pectin, gum arabic (arabic gum), xanthan gum (xanthan gum), locust bean gum (locust bean gum), starch (starch) (e.g. modified starch (modified starch)), trehalose (fucose), dextrin (dextrin) (e.g. Resistant maltodextrin), syrup, guar gum, konjac powder, vegetable fiber, synthetic fiber, semi-synthetic fiber -synthetic fiber), sialic acid, and their combination. In a preferred embodiment of the present invention, the second substance is dextrin.

According to the present invention, the second isoelectric point and the first isoelectric point have a pH difference between 0.5 and 3.

In the preferred embodiment of the present invention, the well-known and customary techniques of those skilled in the art can be used [for example, freeze granulation, fluidized bed granulation, or spray drying granulation ( spray drying granulation)] to form the intermediate layer 12 on the outer surface of the core 11, and form the coating layer 13 on the outer surface of the intermediate layer 12 to protect the probiotics in the core 11, Avoid it being destroyed by stomach acid.

According to the present invention, the probiotic particles 1 with a core-shell structure can be manufactured into a dosage form suitable for oral administration by using techniques well known to those skilled in the art, including, but not limited to: tablets, tablets Troche, lozenge, pellet, hard capsule, soft capsule (for example, crystal ball capsule), dispersible powder or granule ), solutions and the like.

Referring to FIG. 2, another preferred embodiment of the present invention, the probiotic particles 1 having a core-shell structure are processed into a dosage form of a probiotic soft capsule for use as a health food. The probiotic soft capsule includes a capsule shell 2 and a plurality of probiotic particles 1 with a core-shell structure. The capsule shell 2 forms a closed accommodating space 21 that can accommodate the core-shell structures. Probiotic particles 1.

Due to the layered coating structure of the probiotic particles 1 with a core-shell structure of the present invention, the probiotics can aggregate into clumps in an acidic environment to avoid being affected by gastric acid and reduce their survival rate. In addition, it was discovered that the new type of probiotic particles 1 with a core-shell structure has excellent dispersibility in neutral and alkaline environments, so it can quickly disperse after reaching the intestinal tract to play a role, thereby maintaining the human intestinal tract health.

In summary, the invention can effectively reduce the destruction of probiotics caused by gastric acid, so it can indeed achieve the purpose of the cost novel.

However, the above-mentioned are only examples of the present model, and should not be used to limit the scope of implementation of the present model, all simple equivalent changes and modifications made in accordance with the patent scope of the present model application and the contents of the patent specification still belong to This new patent covers the scope.

1: Probiotic particles with core-shell structure 11: core 12: Middle layer 13: Cladding 2: capsule shell 21: Housing space

Other features and effects of the present invention will be clearly presented in the embodiments with reference to the drawings, in which: Figure 1 is a cross-sectional side view illustrating a preferred embodiment of the novel probiotic particles with a core-shell structure of the present invention; and Fig. 2 is a cross-sectional side view illustrating the probiotic soft capsule prepared by the new type of probiotic particles with a core-shell structure.

1: Probiotic particles with core-shell structure

11: core

12: Middle layer

13: Cladding

Claims (6)

A probiotic particle with a core-shell structure, which contains: A core, which contains at least one probiotic; An intermediate layer formed on the outer surface of the core, containing a first substance, the first substance having a first isoelectric point ranging from pH 1 to pH 6; and A coating layer formed on the outer surface of the intermediate layer includes a second substance having a second isoelectric point in the range of pH 4 to pH 8 and higher than the first isoelectric point point, Wherein, the second isoelectric point and the first isoelectric point have a pH difference between 0.5 and 3. The probiotic particle of claim 1, wherein the probiotic is selected from the group consisting of Bacillus species, Streptococcus species, Lactococcus species, auxotrophic bacteria species, Aerococcus species , Carnivora species, Enterococcus species, Lactobacillus species, Leuconostoc species, Ocnococcus species, Micrococcus species, Tetragenococcus species, Heterococcus species, Weissella species Species, Bifidobacterium species, Saccharomyces species, Kluyveromyces species, Staphylococcus species, Pediococcus species, Propionibacterium species, and combinations thereof. The probiotic particles of claim 1, wherein the first substance is selected from the group consisting of skimmed milk powder, casein, whey protein, soy protein, pea protein, egg protein, rice protein, hydrolyzed Protein, zein, wheat gluten, barley protein, branched chain amino acid, gelatin, collagen, amino acid, chitosan, chitosan, and combinations thereof. The probiotic particles of claim 1, wherein the second substance is selected from the group consisting of: sodium alginate, agar gum, carrageenan, pectin, acacia gum, sanxian gum, locust bean Gum, starch, trehalose, dextrin, syrup, Guanhua bean gum, konjac powder, plant fiber, synthetic fiber, semi-synthetic fiber, sialic acid, and combinations thereof. The probiotic particle according to claim 1, which further comprises a pharmaceutically acceptable carrier. A probiotic soft capsule, which contains: A capsule shell, which forms a closed accommodating space; and A plurality of probiotic particles with a core-shell structure are contained in the containing space.

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