TWI908161B - Manufacture method for seaweed fertilizer - Google Patents

Abstract

The present invention provides a manufacture method for seaweed fertilizer. The manufacture method for seaweed fertilizer comprises: step S10: Utilizing compound algae cultivation equipment to cultivate multiple types of algae simultaneously, the compound algae cultivation equipment includes a plurality of reactors to individually cultivate the plurality of types of algae; step S20: Providing a plant nutrient solution required by a target plants to the plurality of algae, causing the algae to adsorb the nutrient solution, so as to obtain at least a first algae stock solution and a second algae stock solution; step S30: adjusting the ratio of the first algae stock solution and the second algae stock solution according to the pH value required by the target plant to obtain an algae mixture; and step S40: diluting the algae mixture by vacuum concentration and homogenizing the algae mixture to obtain the liquid algae fertilizer.

Description

Method for manufacturing liquid algae fertilizer

This invention relates to a method for manufacturing a liquid algae fertilizer, and more particularly to a method for manufacturing a nano-scale liquid algae fertilizer.

Organic fertilizers are made from animal or plant-based organic matter. When applied, they are broken down into inorganic substances by microorganisms in the soil and provided to plant roots for absorption, offering nutrients slowly but persistently. However, most existing organic fertilizers are fermented fertilizers, which cannot mitigate the problems of global warming.

In recent years, seaweed fertilizers made from seaweed have been developed. Seaweed extracts are rich in various amino acids (such as glutamic acid, aspartic acid, and arginine), various plant growth substances (such as auxins, cytokinins, gibberellin, abscisic acid, ethylene, betaine, and alginic acid), and abundant nutrients (such as ascorbic acid, vitamin K, carotene, vitamin _B1 , vitamin _B2 , and vitamin E), providing excellent growth effects for plants. However, seaweed is relatively difficult to obtain and also poses a problem of soil salinization.

Therefore, freshwater algae are being used instead of seaweed fertilizer, as they are easier to cultivate and contain the same nutrients. However, it's important to note that the production process requires additional steps of yeast fermentation and cellulose cell wall breaking to release the nutrients into the liquid fertilizer. Furthermore, if the algae are not properly inactivated, they can easily cause environmental pollution, making the production process more complex and posing risks in its use.

Furthermore, most algae have low requirements for growth conditions, and these marine algae can serve as economically efficient substrates for obtaining high-value-added complexes. They are rich in sugars and proteins, and the processing of various algae can provide abundant raw materials for industries. However, some algae have thick cell walls, making it difficult for the human body to absorb and utilize their nutrients and active ingredients. The organic matter produced during fermentation processes can also have an impact on the local ecosystem due to the accompanying microbial communities. This is also an issue that needs to be addressed.

Therefore, how to save on manufacturing costs and increase the concentration of liquid algae fertilizer through improvements in the manufacturing process has become one of the important issues that this business aims to address.

The technical problem to be solved by this invention is to provide a method for manufacturing liquid algae fertilizer to address the shortcomings of existing technologies.

To solve the above-mentioned technical problems, one of the technical solutions adopted by the present invention is to provide a method for manufacturing a liquid algae fertilizer, which includes the following steps: Step S10: using a composite algae cultivation device to simultaneously cultivate multiple species of algae, the composite algae cultivation device including multiple reactors to individually cultivate the multiple species of algae; Step S20: providing the multiple species of algae with a plant growth regulator required by the target plant. The process involves: S1) preparing a nutrient solution for the algae to absorb the nutrient solution, thereby obtaining at least a first algae stock solution and a second algae stock solution; S2) adjusting the ratio of the first algae stock solution and the second algae stock solution according to the pH value required by the target plant, thereby obtaining an algae mixture; and S3) subjecting the algae mixture to a dilution vacuum concentration process and a homogenization process, thereby obtaining the liquid algae fertilizer.

In one embodiment of the present invention, the algae are Chlorella, Spirulina, Gracilaria, Porphyra, Chlorella, Chlamydomonas acidophilus, and Phaeodactylum tricornutum.

In one embodiment of the present invention, step S20 further includes providing the algae with an algal growth composition.

In one embodiment of the present invention, the method for manufacturing the liquid algae fertilizer further includes inactivating the liquid algae fertilizer to form an organic matter pre-culture medium.

In one embodiment of the present invention, the method for manufacturing the liquid algae fertilizer further includes adding yeast liquid, acetic acid bacteria and lactic acid bacteria mixture to the organic matter pre-culture medium for cultivation to obtain an organic matter solution.

In one embodiment of the present invention, the method for manufacturing the liquid algae fertilizer further includes vacuum concentrating the organic matter solution and then sterilizing it to obtain organic nutrients.

In one embodiment of the invention, the homogenization process is carried out at a pressure of 2 atmospheres.

In one embodiment of the present invention, the homogenization process is carried out at a temperature of 120°C.

In one embodiment of the present invention, the ratio of the first algae stock solution to the second algae stock solution is 1:3 to 3:1.

In one embodiment of the present invention, the liquid algae fertilizer is a nano-sized liquid algae fertilizer.

One of the beneficial effects of this invention is that the method for manufacturing liquid algae fertilizer provided by this invention can simplify the manufacturing process of liquid algae fertilizer and provide nutrient-sufficient liquid algae fertilizer by using a composite algae cultivation equipment to simultaneously cultivate multiple species of algae and providing the multiple species of algae with a plant nutrient solution required by a target plant, so that the algae absorb the nutrient solution.

To further understand the features and technical content of this invention, please refer to the following detailed description and drawings of this invention. However, the drawings provided are for reference and illustration only and are not intended to limit this invention.

The following specific embodiments illustrate the implementation of the "method for manufacturing liquid algae fertilizer" disclosed in this invention. Those skilled in the art can understand the advantages and effects of this invention from the content disclosed in this specification. This invention can be implemented or applied through other different specific embodiments, and various details in this specification can also be modified and changed based on different viewpoints and applications, without departing from the concept of this invention. Furthermore, the accompanying drawings of this invention are for simple illustrative purposes only and are not depictions based on actual dimensions, as stated in advance. The following embodiments will further explain the relevant technical content of this invention in detail, but the disclosed content is not intended to limit the scope of protection of this invention.

It should be understood that although terms such as "first," "second," and "third" may be used in this document to describe various components or signals, these components or signals should not be limited by these terms. These terms are primarily used to distinguish one component from another, or one signal from another. Furthermore, the term "or" used in this document should, as appropriate, include any combination of one or more of the related listed items.

Referring to Figure 1, a first embodiment of the present invention provides a method for manufacturing a liquid algae fertilizer, which includes at least the following steps: Step S10: Simultaneously cultivating multiple species of algae using a composite algae cultivation device, the composite algae cultivation device including multiple reactors to individually cultivate the multiple species of algae; Step S20: Providing the multiple species of algae with a plant nutrient solution required by a target plant. The algae adsorb the nutrient solution to obtain at least a first algae stock solution and a second algae stock solution; step S30: adjust the ratio of the first algae stock solution and the second algae stock solution according to the pH value required by the target plant to obtain an algae mixture; and step S40: subject the algae mixture to a dilution vacuum concentration treatment and a homogenization treatment to obtain the liquid algae fertilizer.

It should be noted that the composite algae cultivation equipment of this invention can adopt, for example, the composite algae cultivation equipment of Taiwan Utility Model Patent No. M630069. This equipment has individual photosynthetic reaction units and growth tank units that can be separated independently, thus meeting the needs of different types of algae and simultaneously cultivating multiple algae species. Furthermore, because the composite algae cultivation equipment combines a tubular photosynthetic reaction unit with a growth tank unit whose capacity is several times that of the photosynthetic reaction unit, it combines the strong photosynthetic reaction of the tubular photosynthetic reactor with the large capacity and growth regulation function of the growth tank, thereby achieving the goal of increasing yield and improving quality. The composite algae cultivation equipment will be described in detail below.

Please refer to Figure 2. The composite algae cultivation equipment 100 includes a photosynthetic reaction module 1, a growth regulation module 2, a circulation conveying module 3, a circulation pipeline module 4, a growth monitoring and regulation module 5, and a control module (not shown). The growth regulation module 2 includes a plurality of growth tank units 20 corresponding to the plurality of photosynthetic reaction units 10. The multiple growth tank units 20 of the growth regulation module 2 are connected to the multiple photosynthetic reaction units 10 of the photosynthetic reaction module 1 through the circulation pipeline module 4. Through the control of the circulation transport module 3 and the circulation pipeline module 4, the culture medium flowing out of the multiple photosynthetic reaction units 10 of the photosynthetic reaction module 1 can enter the multiple growth tank units 20, and the culture medium discharged from each growth tank unit 20 can also be recycled back into the multiple photosynthetic reaction units 10 of the photosynthetic reaction module 1.

The photosynthetic reaction module 1 includes multiple photosynthetic reaction units 10. Each photosynthetic reaction unit 10 has an inlet end 111 and an outlet end 112 at both ends. The culture medium for cultivating algae can enter the photosynthetic reaction unit 10 from the inlet end 111 and pass through the photosynthetic reaction unit 10 at a stable flow rate. The algae in the culture medium carry out photosynthesis in the photosynthetic reaction unit 10, thereby obtaining nutrients and growing. The two first inlet bypass connectors 16 and the first outlet bypass connector 17 of each photosynthetic reaction unit 10 are respectively disposed at the inlet end 111 and the outlet end 112 of the photosynthetic reaction unit 10. The purpose of the first inlet bypass connector 16 and the first outlet bypass connector 17 is to connect the pipeline of the external circulation equipment for cleaning the photosynthetic reaction unit 10 or for use in mixed culture of other algae.

Each growth tank unit 20 has a tank body 21 with a growth tank inlet 211 and a growth tank outlet 212. The culture medium enters the tank body 21 from the growth tank inlet 211 and flows out from the growth tank outlet 212. In particular, the volume of the tank body 21 of each growth tank unit 20 is arranged to be several times larger than the volume of the photosynthetic reaction unit 10, and the residence time of the culture medium in the growth tank unit 20 is also arranged to be greater than the residence time of the culture medium in the photosynthetic reaction unit 10. Therefore, the growth tank unit 20 can provide a volume several times that of the photosynthetic reaction unit 10, thus enabling the composite algae cultivation equipment 100 to overcome the problem of the small volume of existing closed photosynthetic reactors or reaction tanks.

Furthermore, during the flow of the culture medium within the tank 21 of the growth tank unit 20, the temperature of the culture medium can be gradually reduced, and the light intensity can be gradually decreased. This allows the algae in the culture medium to rapidly multiply through photosynthesis in the photosynthetic reaction unit 10, and then gradually cool down and slow down photosynthesis after entering the growth tank unit 20. This gives the algae in the culture medium sufficient time to digest the nutrients obtained in the previous photosynthetic reaction unit 10, and allows the algae to further divide after growing to a certain size, resulting in a doubling of algal reproduction and sufficient growth regulation, thereby improving the quality of the produced algae.

In addition, each growth tank unit 20 may also include a second inlet bypass connector 26 and a second outlet bypass connector 27. The second inlet bypass connector 26 and the second outlet bypass connector 27 are respectively connected to the growth tank inlet 211 and the growth tank outlet 212 of the tank body 21. When each growth tank unit 20 is being cleaned or when other types of algae need to be temporarily mixed, the cleaning pipeline or the pipeline of the external circulation equipment used for mixed cultivation can be connected to the second inlet bypass connector 26 and the second outlet bypass connector 27.

Each growth tank unit 20 can also be equipped with a nutrient supply bottle 215. The nutrient supply bottle 215 is connected to a gas distribution pipe 534, and can deliver the supplementary material in the nutrient supply bottle 215 into the growth tank unit 20 through air pressure control, or sample the culture medium in the growth tank unit 20 for functions such as nutrient supplementation, gas addition, and sampling investigation. Furthermore, when the growth tank unit 20 is used to co-culture different algae, each growth tank unit 20 can be equipped with a dedicated nutrient supply bottle 215 for supplementing specific nutrients and gases to ensure no contamination.

Before proceeding to step S10, the algae can be disinfected, soaked, and cleaned. Step S10 involves the cultivation process using a composite algae cultivation system. In this system, the algae to be cultivated are selected based on the needs of the target plant, and corresponding reactors are allocated according to the different cultivation rates of each type of algae. Specifically, the composite algae cultivation system can provide different cultivation temperatures and/or illuminance for each type of algae, and by increasing the growth components required by the algae, it increases the amount of nutrients obtained by the algae. It can also enhance photosynthesis, adjust the algae's light sensitivity, and thus increase the algae's growth rate.

In this invention, it is preferred to use freshwater algae as the raw material for liquid algae fertilizer. For example, the raw materials for liquid algae fertilizer may be Chlorella, Spirulina, Gracilaria, Porphyra (neutral), Chlorella (neutral), Chlamydomonas (acidic), and Phaeodactylum tricornutum (alkaline), etc. However, the above examples are only one feasible embodiment and are not intended to limit this invention.

In step S20, algae growth compounds can be added according to the specific needs of different algae. For example, spirulina can use the Zarrouk formula, the detailed ingredients of which are shown in Table 1 below, and the detailed ingredients of A5 and B6 are shown in Table 2 below: Table 1 Chemical composition content _NaHCO3 (1) 16.80 g/L _K₂HPO₄ (4) 0.50 g/L _NaNO3 (2) 2.50 g/L NaCl(3) 1.00 g/L _MgSO₄ · _7H₂O (6) 0.20 g/L _FeSO₄ · _7H₂O (8) 0.01 g/L _K₂SO₄ ( ₅ ) 1.00 g/L _CaCl₂ · _2H₂O 0.04 g/L EDTA(8) 0.08 g/L A5(9) 1 ml/L B6(10) 1 ml/L Table 2 Chemical composition Content (per 1000 mL) A5 H ₃ BO ₃ 2.86g _{( NH4} ) _6Mo7O24 0.02g _MnCl₂ · _4H₂O 1.80g _CuSO₄ · _5H₂O 0.08g _ZnSO₄ · _7H₂O 0.22g B6 _{NH4VO3 ​} 22.9g _NiSO₃ · _7H₂O 47.8g NaWO ₄ 17.9g Ti( _SO₄ ) _₂ 40.0g Co( _NO3 ) ₂ · _6H2O 4.4g

In another embodiment of the present invention, the BG11 formula is used when culturing Chlorella and Staphylococcus aureus. The detailed composition of the BG11 formula is shown in Table 3 below: Table 3 Chemical composition content NaNO ₃ 1.5 g/L _{K₂HPO₄ · 3H₂O} 0.04 g/L MgSO4 _{· 7H2O} 0.075 g/L _CaCl₂ · _2H₂O 0.036 g/L Citric acid 0.006 g/L Ferric ammonium citrate 0.006 g/L EDTA (dinatrium-salt) 0.001 g/L _{Na₂CO₃ ​} 0.02 g/L A5 + Co solution 1ml

It is worth noting that this invention adds the plant nutrient solution required by the target plant during the algae cultivation stage, allowing the algae to absorb the nutrients needed by the target plant while growing. In this way, the algae can convert inorganic salts into organic matter. Furthermore, because this invention uses a composite algae cultivation device, different species of algae can grow in the same environmental system, and then their growth rates can be adjusted by differential temperature regulation. Therefore, even if the nutrients required by the target plant are added at this stage, the growth rate of the algae can be maintained through differential temperature regulation. For example, Spirulina grows slower than Chlorella, but Spirulina can tolerate higher temperatures, so they can grow in the same environmental system and then have their growth rates adjusted accordingly.

In one embodiment of the invention, the target plant is tomato (tomatoes thrive at pH 6.0-7.0), and the plant nutrient solution may include 2 g to 6 g of potassium sulfate, 25 mg to 150 mg of borax, 25 mg to 150 mg of manganese sulfate, and 50 mg to 300 mg of ammonium molybdate. In another embodiment of the invention, the target plant is citrus (citrus thrives at pH 6.5-7.5), and the plant nutrient solution may include 2.5 g to 4.5 g of calcium nitrate, 25 mg to 150 mg of borax, 25 mg to 200 mg of manganese sulfate, and 25 mg to 100 mg of zinc sulfate. In yet another embodiment of the invention, the target plant is lychee (lychee is suitable for pH 6.0-6.5), and the plant nutrient solution may include 20 mg to 60 mg of borax, 100 mg to 20 mg of manganese sulfate and 15 mg to 30 mg of zinc sulfate.

Because the algae stock solutions obtained from the aforementioned steps have different characteristics, the proportions of different algae stock solutions can be adjusted according to the suitable pH value and nutrient requirements of the target plant, and the required proportion of organic matter can be added to change the pH concentration. Taking Chlorella as an example, Chlorella grows quickly, but its nutrient content is not as abundant as that of Spirulina. While Spirulina is rich in nutrients, it is alkaline and can easily make overly alkaline soil even more saline, but it can neutralize acidic soil.

In one embodiment of the present invention, the ratio of the first algae stock solution to the second algae stock solution is 1:3 to 3:1. However, in another embodiment of the present invention, only one algae stock solution may be used to prepare the liquid algae fertilizer. Specifically, the pH adjustment method follows the following rules: (1) If c(H+) or c(OH－) differs (changes) by a factor of 10, the pH differs (changes) by 1 unit; (2) If a strong acid pH=a, adding a neutral solution to dilute by 10n will result in pH=a+n; if a weak acid pH=a, adding a neutral solution to dilute by 10n will result in pH<a+n, but definitely greater than a; (3) If a strong base pH=b, adding a neutral solution to dilute by 10n will result in pH=b-n; if a weak base pH=b, adding a neutral solution to dilute by 10n will result in pH>b-n, but definitely less than b.

In one embodiment of this invention, when the target plant is a tomato, since tomatoes thrive in slightly acidic to neutral soil, a mixture of Chlorella stock solution and Spirulina stock solution in a 3:1 ratio can be used to obtain an algae mixture with a pH of approximately 5.0. When the target plant is a citrus, only Chlorella stock solution can be used, resulting in a pH of approximately 7.0. When the target plant is a lychee, since lychees thrive in slightly acidic soil, a mixture of Chlorella stock solution and Spirulina stock solution in a 3:1 ratio can be used to obtain an algae mixture with a pH of approximately 5.0. Furthermore, yeast solution (pH 4.5-5.0), acetic acid bacteria solution (pH 5.0-6.0), and lactic acid bacteria solution (pH 6.0-7.0) are all slightly acidic solutions. During preparation, the required proportion of acidic to neutral algae solution can be reduced, and these three organic solvents can be used as substitutes. If the plants require alkaline algae fertilizer, the organic solvent should be titrated to neutral before being added.

It is worth noting that the prepared algae mixture can be directly diluted, vacuum concentrated and homogenized in step S40 without the need for traditional cell wall breaking and enzymatic hydrolysis, thus accelerating the production process of liquid algae fertilizer and saving manufacturing costs.

In step S40, the algae mixture obtained after algae cultivation can be directly diluted and vacuum concentrated. The dilution and vacuum concentration treatment conditions are 0.3 atmospheres and a temperature of 70°C for 12 hours. Because the dilution and vacuum concentration treatment can increase the concentration of the algae mixture, it can increase the concentration of organic matter and other nutrients in the produced liquid algae fertilizer, thereby improving the utilization efficiency of the liquid algae fertilizer and reducing its transportation costs.

Furthermore, the concentrated algae mixture can be homogenized to produce a nano-sized liquid algae fertilizer. Specifically, homogenization damages the algae cells in the mixture, causing peptides, enzymes, and other substances within the algae to migrate out of the cells and break down into particles with a diameter of less than ^10⁻⁹ μm. Moreover, the homogenization process of this invention is carried out under high pressure, such as 2 atmospheres in a homogenizer, to compress the algae cells, causing them to break down and inactivate, thus avoiding environmental pollution caused by the use of algae fertilizer. In one embodiment of this invention, the homogenization process avoids high temperatures that could damage the enzymes and nutrients within the algae. S10~S40 are steps that involve fermenting algal cellular components to obtain organic matter.

Furthermore, a second embodiment of the present invention provides a method for manufacturing liquid algae fertilizer with increased organic matter content. Referring to Figure 3, a method for manufacturing liquid algae fertilizer with an organic matter content of 200 g/L can be provided. Specifically, using microbial culture as a supplementary method can increase the upper limit of organic matter in the algae mixture to approximately 300 g/L; if microbial culture is not used and no concentration is performed, the lower limit of organic matter is approximately 1 g/L. That is, the organic matter content can be controlled between 1 g/L and 300 g/L.

In detail, in step J10, the liquid algae fertilizer can be inactivated to form an organic matter pre-culture medium. The inactivation treatment involves placing the liquid algae fertilizer obtained from step S40 into an incubator at a pressure of 2 atmospheres and a temperature of 120°C for 15 minutes. This process, similar to steaming, transforms the raw liquid algae fertilizer into cooked liquid algae fertilizer, which can then be used as an organic matter pre-culture medium.

Given that the conversion of plants into organic matter is carried out through fermentation by microorganisms (e.g., bacteria), fermenting (enzymatic hydrolysis) algae helps to process and utilize the nutrients and active ingredients of algae, that is, to utilize the organic matter that is formed after enzymatic hydrolysis into primary or secondary structures.

Specifically, in step J20, a mixture of yeast, acetic acid bacteria, and lactic acid bacteria is added to the organic matter pre-culture medium for cultivation to obtain an organic matter solution. It should be noted that a 20% molasses solution can be used to prepare the yeast, acetic acid bacteria, and lactic acid bacteria mixture, i.e., 1 kg of molasses is mixed with yeast, acetic acid bacteria, and lactic acid bacteria. In one embodiment of the invention, 200 g of yeast, 200 g of acetic acid bacteria, and 100 g of lactic acid bacteria are mixed to form 500 g of live bacteria solution, which is then added to 2 kg of organic matter pre-culture medium to prepare a 25% mixture. The mixture is then further cultured for 15 days at pH 5.5-6.5 and a temperature of 25°C-35°C to form an organic matter solution (2.5 kg). In this step, the ability of bacteria to break down the quaternary structure of proteins into amino acids is utilized to produce organic matter, mimicking the fermentation reaction in nature.

For example, the fermentation reaction of lactic acid bacteria _, which converts protein into organic matter and energy, is _C6H12O6 + _2ADP + 2Pi → + _2CH3CH (OH)COOH + 2ATP. The fermentation reaction of yeast _{, which converts protein into organic matter, energy, and carbon dioxide, is C6H12O6 → 2C2H5OH + 2CO2 + ATP} . The fermentation reaction of acetic acid bacteria, which converts protein and oxygen into organic matter, water, and carbon dioxide _, is _C6H12O6 + _2O2 → _2CH3COOH + _2CO2 + _2H2O . The fermentation reaction of lactic _{acid bacteria, which ferments sugars into lactic acid and energy, is C6H12O6 + enzyme → 2C3H6O3 + a small} amount _of energy. The fermentation reaction of acetic _acid bacteria into alcohol _{is C6H12O6} + enzyme → _2C2H5OH + _2CO2 + a small amount _of energy. Most bacteria can ferment cellulose into sugar using the reaction _C6H10O5 + _H2O = _C6H12O6 . Most bacteria _{can also} ferment _{fats into} alcohol using the reaction _{C3H8O3 + 3CnH2nO2} → _CnH2n ( _{C3H5O ) n + 3 + 3H2O .}

Furthermore, the liquid algae fertilizer (raw algae) obtained from step S40 and the organic matter pre-culture medium (cooked algae) after inactivation treatment were used to cultivate yeast, acetic acid bacteria, and lactic acid bacteria, respectively. 40 ml of molasses and 200 ml of mineral water were added to each of the three culture flasks, followed by the addition of 40 g of yeast, 40 g of acetic acid bacteria, and 40 g of lactic acid bacteria to each flask. The flasks were then incubated in a constant temperature water bath at 25°C for seven days. After seven days, the incubation was mixed with the algae solution, and the mass fraction of organic matter was analyzed. The results are shown in Table 4 below: Table 4 bacterial solution Organic matter mass fraction (g/L) Implementation Example 1 Cooked algae acetic acid bacteria solution 24.8 Implementation Example 2 Cooked algae yeast liquid 21.9 Implementation Example 3 Cooked algae lactic acid bacteria liquid 16.7 Comparative example 1 Algae-containing acetic acid bacteria solution 10.0 Comparative example 2 Algae Yeast Liquid 22.4 Comparative example 3 Algae-based lactic acid bacteria liquid 10.8

The results shown in Table 4 above confirm that inactivating the liquid algae fertilizer obtained in step S40 as described in step J10 can increase the content of organic matter obtained subsequently. The reason why there is little difference in organic matter between cooked and raw algae yeast liquid is that some components in the protein cellulose decomposition structure have not disappeared, which is an adaptation of the fungi, indicating that yeast has strong adaptability.

Furthermore, the addition of more and better microbial communities will trigger more fermentation reactions based on their advantages in decomposing components, resulting in the acquisition of more organic matter. However, the organic matter must be inactivated after acquisition, otherwise it is easy to pollute the environment or damage the bacterial quantity and bacterial composition of the place of use.

Finally, in step J30, the organic matter solution is vacuum concentrated and then sterilized (i.e., high-temperature inactivation of bacteria) to obtain organic nutrients. Specifically, vacuum compression drying can be performed at a temperature of 70°C and a pressure of 0.3 atmospheres until the weight continuously decreases to 50% (i.e., 1.25kg), forming 50% organic nutrients. Furthermore, in step S41, this organic nutrient can be added to the liquid algae fertilizer obtained in step S40 according to the required ratio to obtain an organic liquid algae fertilizer rich in organic matter and active substances. This allows for the customized production of liquid algae fertilizer with organic matter content ranging from 1g/L to 300g/L. In addition, the vacuum compression drying process can also remove gases such as ammonia generated in step J20, thus preventing the odor from spreading into the manufacturing environment.

In other words, step S41 can involve adding the appropriate fermentation solution to the liquid algae fertilizer (liquid culture medium) obtained from step S40, mixing it, and culturing it under suitable conditions for 10 to 15 days to form an organic solvent. This solvent is then vacuum-concentrated by 50% and subsequently inactivated. Finally, organic nutrients are added according to the proportions required by the target plants to generate an organic liquid algae fertilizer rich in organic matter and active substances. The aforementioned suitable conditions can be those appropriate for the fermentation reaction.

[Beneficial effects of the implementation]

One of the beneficial effects of this invention is that the method for manufacturing liquid algae fertilizer provided by this invention can simplify the manufacturing process of liquid algae fertilizer and provide nutrient-sufficient liquid algae fertilizer by using a composite algae cultivation equipment to simultaneously cultivate multiple species of algae and providing the multiple species of algae with a plant nutrient solution required by a target plant, so that the algae absorb the nutrient solution.

Furthermore, this invention utilizes a composite algae cultivation device that can separate different temperatures and different nutrient salts to simultaneously cultivate different species of algae while increasing their growth rate. By tailoring the algae growth composition to the specific needs of different algae species and adjusting their light sensitivity, photosynthesis can also be enhanced. Therefore, even if the nutrient solution required by the target plant is added during the cultivation stage, it will not affect the growth of the algae.

It is worth mentioning that the method for manufacturing liquid algae fertilizer of this invention adds the nutrients required by the target plant to the algae during the algae cultivation stage. By utilizing the adsorption properties of algae, the nutrients are absorbed into the algae cells. Compared with the existing technology, which adds the nutrients required by the target plant after obtaining the algae liquid, this method can obtain liquid algae fertilizer with a higher concentration of nutrients and simplifies the production process of liquid algae fertilizer (for example, omitting the extraction and filtration steps), thus saving manufacturing costs.

Furthermore, the liquid algae fertilizer of this invention can be combined with microbial cultivation to increase the organic matter content of the liquid algae fertilizer. Moreover, in response to the different decomposition of different components and the different amounts of organic matter produced, the proportions of different microbial strains can be adjusted as needed to improve the efficiency of organic matter acquisition.

The above-disclosed content is merely a preferred feasible embodiment of the present invention and is not intended to limit the scope of the patent application of the present invention. Therefore, all equivalent technical changes made using the contents of the present invention's description and drawings are included within the scope of the patent application of the present invention.

1: Photosynthetic Reaction Module 10: Photosynthetic Reaction Unit 16: First Inlet Bypass Connector 17: First Outlet Bypass Connector 111: Water Inlet 112: Water Outlet 2: Growth Regulation Module 20: Growth Tank Unit 21: Tank Body 26: Second Inlet Bypass Connector 27: Second Outlet Bypass Connector 211: Growth Tank Inlet 212: Growth Tank Outlet 215: Nutrient Supply Bottle 3: Circulation Transport Module 4: Circulation Pipeline Module 5: Growth Monitoring and Regulation Module 534: Gas Distribution Pipe S10~S41, J10~J30: Steps

Figure 1 is a flowchart of the manufacturing method of liquid algae fertilizer according to the first embodiment of the present invention.

Figure 2 is a system block diagram of a composite algae cultivation equipment used in the method for manufacturing liquid algae fertilizer of the present invention.

Figure 3 is a flowchart of the manufacturing method of liquid algae fertilizer according to the second embodiment of the present invention.

S10~S40: Steps

Claims (10)

A method for manufacturing a liquid algae fertilizer includes the following steps: Step S10: Simultaneously cultivating multiple species of algae using a composite algae cultivation device, the composite algae cultivation device including multiple reactors to cultivate the multiple species of algae individually; Step S20: Providing the multiple species of algae with a plant nutrient solution required by a target plant, allowing the multiple species of algae to adsorb the nutrient solution, to obtain at least a first algae stock solution and a second algae stock solution; Step S30: Adjusting the ratio of the first algae stock solution and the second algae stock solution according to the pH value required by the target plant, to obtain an algae mixture; and Step S40: Performing a dilution vacuum concentration treatment and a homogenization treatment on the multiple algae mixture to obtain the liquid algae fertilizer. The method for manufacturing liquid algae fertilizer as described in claim 1, wherein the plurality of algae are selected from a group consisting of Chlorella, Spirulina, Botrytis cinerea, Porphyra yezoensis, Chlorella vulgaris, Chlamydomonas acidophilus, and Phaeodactylum tricornutum. The method for manufacturing liquid algae fertilizer as described in claim 1, wherein step S20 further includes providing an algae growth composition to the plurality of algae species. The method for manufacturing liquid algae fertilizer as described in claim 1 further includes inactivating the liquid algae fertilizer to form an organic matter pre-culture medium. The method for manufacturing liquid algae fertilizer as described in claim 4 further includes adding yeast liquid, acetic acid bacteria and lactic acid bacteria mixture to the organic matter pre-culture medium for cultivation to obtain an organic matter solution. The method for manufacturing liquid algae fertilizer as described in claim 5 further includes vacuum concentrating the organic matter solution and then sterilizing it to obtain organic nutrients. The method for manufacturing liquid algae fertilizer as described in claim 1, wherein the homogenization process is carried out at a pressure of 2 atmospheres. The method for manufacturing liquid algae fertilizer as described in claim 1, wherein the homogenization treatment temperature is 120°C. The method for manufacturing liquid algae fertilizer as described in claim 1, wherein the ratio of the first algae stock solution to the second algae stock solution is 1:3 to 3:1. The method for manufacturing liquid algae fertilizer as described in claim 1, wherein the liquid algae fertilizer is a nano-sized liquid algae fertilizer.